MD04G1175300.v1.1

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004770
Physical Location & Seq
Forward (+)
26642451 .. 26643363
913 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1175300.v1.1.491

Sequence Viewer

Length: 675 bp
ATGGAAGTGCTTGTTTATTTAGACACCATTTTAGTCCCCTTGAGCTTCTTCCTTCTGATAGGCTACCATGTCTATCTATGGTACACCTTCAAGGCCAAACCCTCTCTTACCACCATTGGAATTGACTCAATGAGAAGAAGAAAATGGTTTCTAGAAATTATGGAGGCTGATGGTATGAAGGGGATGCTGGTAATCCAAAGCTTGAGAAACACTCAAATGGTGGCAATATTCACAGCTTCTACAGCAATTGCTTTAAGCTTGACTTTGGCAGCTCTCACCAACAATGCTTACAATGCAAGTCACCTCGTTATCAAAAGCCCATTTTTTGGTTCGCAGTCTGGAAGCATCTTTGCTCTGAAATATGGGTTGGCCTCATTTCTCATGTTGTTTAGCTCATTATTCAGCTCCATGGCGACCGGGTTCCTGATCGATACCATTTTTCTAATAAACGCTTCTTTTGAGTTCTCATATTCCGGAATCACAGAAACCCAACTTGAAAAAGGGTACATGTTGGCTCTTATTGGCAACAGGTTGCTTTGTATTTCCTTTCCCATGTTGTTGTGGTTGTTTGGTCCAATGCTTGTTGCATTGTCTTCCCTGGCCTTGGTTTGGTGGCTTTATGAGCTTGATTTTGCTGCCAAACTCAGCACAAGCAGTAGGCAAAGCCTTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

225

Amino Acids

25.06

Weight (kDa)

8.64

Isoelectric Point (pI)

32.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 10 - 213 6.1e-47 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 326
AccIII TCCGGA 1 cut(s) 473
AfaI GTAC 2 cut(s) 83, 506
AfiI CCNNNNNNNGG 3 cut(s) 326, 604, 609
AflIII ACRYGT 1 cut(s) 507
AgsI TTSAA 2 cut(s) 91, 497
AjnI CCWGG 1 cut(s) 597
AjuI GAANNNNNNNTTGG 2 cut(s) 350, 382
AluBI AGCT 8 cut(s) 45, 201, 236, 258, 272, 393, 405, 625
AluI AGCT 8 cut(s) 45, 201, 236, 258, 272, 393, 405, 625
Aor13HI TCCGGA 1 cut(s) 473
AoxI GGCC 3 cut(s) 93, 369, 600
ApeKI GCWGC 2 cut(s) 269, 635
AspS9I GGNCC 1 cut(s) 572
AsuC2I CCSGG 1 cut(s) 418
AsuHPI GGTGA 2 cut(s) 268, 293
AvaII GGWCC 1 cut(s) 572
BbsI GAAGAC 1 cut(s) 585
BbvI GCAGC 2 cut(s) 281, 622
BccI CCATC 1 cut(s) 164
BciT130I CCWGG 1 cut(s) 599
BcnI CCSGG 1 cut(s) 418
BfaI CTAG 1 cut(s) 152
BfmI CTRYAG 1 cut(s) 240
BisI GCNGC 2 cut(s) 270, 636
BlsI GCNGC 2 cut(s) 271, 637
Bme1390I CCNGG 2 cut(s) 418, 599
Bme18I GGWCC 1 cut(s) 572
BmgT120I GGNCC 1 cut(s) 572
BmiI GGNNCC 1 cut(s) 422
BmrFI CCNGG 2 cut(s) 418, 599
BmsI GCATC 2 cut(s) 174, 354
BpiI GAAGAC 1 cut(s) 585
BplI GAGNNNNNCTC 2 cut(s) 196, 228
BpuEI CTTGAG 2 cut(s) 61, 223
BpuMI CCSGG 1 cut(s) 418
Bsa29I ATCGAT 1 cut(s) 429
BsaJI CCNNGG 3 cut(s) 408, 597, 603
BsaWI WCCGGW 1 cut(s) 473
Bsc4I CCNNNNNNNGG 3 cut(s) 326, 604, 609
BseAI TCCGGA 1 cut(s) 473
BseBI CCWGG 1 cut(s) 599
BseCI ATCGAT 1 cut(s) 429
BseDI CCNNGG 3 cut(s) 408, 597, 603
BseGI GGATG 1 cut(s) 189
BseLI CCNNNNNNNGG 3 cut(s) 326, 604, 609
BseMII CTCAG 1 cut(s) 658
BseXI GCAGC 2 cut(s) 281, 622
Bsh1285I CGRYCG 1 cut(s) 417
BshFI GGCC 3 cut(s) 95, 371, 602
BshVI ATCGAT 1 cut(s) 429
BsiEI CGRYCG 1 cut(s) 417
BsiSI CCGG 2 cut(s) 417, 474
BslFI GGGAC 1 cut(s) 20
BslI CCNNNNNNNGG 3 cut(s) 326, 604, 609
BsmFI GGGAC 1 cut(s) 20
BsnI GGCC 3 cut(s) 95, 371, 602
Bsp13I TCCGGA 1 cut(s) 473
Bsp143I GATC 1 cut(s) 426
Bsp19I CCATGG 1 cut(s) 408
BspANI GGCC 3 cut(s) 95, 371, 602
BspCNI CTCAG 1 cut(s) 657
BspDI ATCGAT 1 cut(s) 429
BspEI TCCGGA 1 cut(s) 473
BspLI GGNNCC 1 cut(s) 422
BssECI CCNNGG 3 cut(s) 408, 597, 603
BssMI GATC 1 cut(s) 426
BssT1I CCWWGG 2 cut(s) 408, 603
Bst2UI CCWGG 1 cut(s) 599
BstDEI CTNAG 2 cut(s) 644, 668
BstDSI CCRYGG 1 cut(s) 408
BstF5I GGATG 1 cut(s) 189
BstKTI GATC 1 cut(s) 429
BstMBI GATC 1 cut(s) 426
BstMCI CGRYCG 1 cut(s) 417
BstMWI GCNNNNNNNGC 3 cut(s) 242, 293, 622
BstNI CCWGG 1 cut(s) 599
BstNSI RCATGY 1 cut(s) 511
BstSCI CCNGG 2 cut(s) 416, 597
BstSFI CTRYAG 1 cut(s) 240
BstV1I GCAGC 2 cut(s) 281, 622
BstV2I GAAGAC 1 cut(s) 585
Bsu15I ATCGAT 1 cut(s) 429
BsuRI GGCC 3 cut(s) 95, 371, 602
BsuTUI ATCGAT 1 cut(s) 429
BtgI CCRYGG 1 cut(s) 408
BtsCI GGATG 1 cut(s) 189
Cfr13I GGNCC 1 cut(s) 572
ClaI ATCGAT 1 cut(s) 429
Csp6I GTAC 2 cut(s) 82, 505
CviAII CATG 5 cut(s) 68, 382, 409, 508, 553
CviQI GTAC 2 cut(s) 82, 505
DdeI CTNAG 2 cut(s) 644, 668
DpnI GATC 1 cut(s) 428
DpnII GATC 1 cut(s) 426
Eco130I CCWWGG 2 cut(s) 408, 603
Eco47I GGWCC 1 cut(s) 572
EcoRII CCWGG 1 cut(s) 597
EcoT14I CCWWGG 2 cut(s) 408, 603
ErhI CCWWGG 2 cut(s) 408, 603
FaeI CATG 5 cut(s) 71, 385, 412, 511, 556
FalI AAGNNNNNCTT 2 cut(s) 247, 279
FaqI GGGAC 1 cut(s) 20
FatI CATG 5 cut(s) 67, 381, 408, 507, 552
Fnu4HI GCNGC 2 cut(s) 270, 636
FokI GGATG 1 cut(s) 196
Fsp4HI GCNGC 2 cut(s) 270, 636
FspBI CTAG 1 cut(s) 152
GluI GCNGC 2 cut(s) 270, 636
HaeIII GGCC 3 cut(s) 95, 371, 602
HapII CCGG 2 cut(s) 417, 474
Hin1II CATG 5 cut(s) 71, 385, 412, 511, 556
HindIII AAGCTT 2 cut(s) 199, 256
HinfI GANTC 2 cut(s) 125, 477
HpaII CCGG 2 cut(s) 417, 474
HphI GGTGA 2 cut(s) 268, 293
Hpy166II GTNNAC 1 cut(s) 84
Hpy188I TCNGA 2 cut(s) 57, 357
Hpy188III TCNNGA 4 cut(s) 152, 339, 424, 474
Hpy8I GTNNAC 1 cut(s) 84
HpyAV CCTTC 3 cut(s) 62, 97, 172
HpyCH4V TGCA 2 cut(s) 296, 587
HpyF10VI GCNNNNNNNGC 3 cut(s) 242, 293, 622
HpyF3I CTNAG 2 cut(s) 644, 668
Hsp92II CATG 5 cut(s) 71, 385, 412, 511, 556
Kpn2I TCCGGA 1 cut(s) 473
Kzo9I GATC 1 cut(s) 426
LmnI GCTCC 1 cut(s) 410
LpnPI CCDG 8 cut(s) 173, 324, 430, 437, 487, 514, 584, 611
Lsp1109I GCAGC 2 cut(s) 281, 622
LweI GCATC 2 cut(s) 174, 354
MaeI CTAG 1 cut(s) 152
MaeIII GTNAC 1 cut(s) 299
MalI GATC 1 cut(s) 428
MboI GATC 1 cut(s) 426
MboII GAAGA 4 cut(s) 40, 147, 150, 585
MfeI CAATTG 1 cut(s) 246
MluCI AATT 3 cut(s) 120, 156, 246
MlyI GAGTC 1 cut(s) 119
MnlI CCTC 4 cut(s) 112, 157, 314, 382
MroI TCCGGA 1 cut(s) 473
MseI TTAA 1 cut(s) 254
MslI CAYNNNNRTG 1 cut(s) 215
MspI CCGG 2 cut(s) 417, 474
MspR9I CCNGG 2 cut(s) 418, 599
MunI CAATTG 1 cut(s) 246
MvaI CCWGG 1 cut(s) 599
MwoI GCNNNNNNNGC 3 cut(s) 242, 293, 622
NciI CCSGG 1 cut(s) 418
NcoI CCATGG 1 cut(s) 408
NdeII GATC 1 cut(s) 426
NlaIII CATG 5 cut(s) 71, 385, 412, 511, 556
NlaIV GGNNCC 1 cut(s) 422
NmuCI GTSAC 1 cut(s) 299
NspI RCATGY 1 cut(s) 511
PciI ACATGT 1 cut(s) 507
PfeI GAWTC 1 cut(s) 477
PflMI CCANNNNNTGG 1 cut(s) 326
PkrI GCNGC 2 cut(s) 271, 637
PleI GAGTC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 119
PscI ACATGT 1 cut(s) 507
Psp6I CCWGG 1 cut(s) 597
PspGI CCWGG 1 cut(s) 597
PspN4I GGNNCC 1 cut(s) 422
PspPI GGNCC 1 cut(s) 572
RsaI GTAC 2 cut(s) 83, 506
RsaNI GTAC 2 cut(s) 82, 505
RseI CAYNNNNRTG 1 cut(s) 215
SaqAI TTAA 1 cut(s) 254
SatI GCNGC 2 cut(s) 270, 636
Sau3AI GATC 1 cut(s) 426
Sau96I GGNCC 1 cut(s) 572
SchI GAGTC 1 cut(s) 119
ScrFI CCNGG 2 cut(s) 418, 599
SfaNI GCATC 2 cut(s) 174, 354
SfcI CTRYAG 1 cut(s) 240
SinI GGWCC 1 cut(s) 572
SmiMI CAYNNNNRTG 1 cut(s) 215
SmlI CTYRAG 2 cut(s) 40, 202
SmoI CTYRAG 2 cut(s) 40, 202
Sse9I AATT 3 cut(s) 120, 156, 246
SspI AATATT 1 cut(s) 228
SspMI CTAG 1 cut(s) 152
StyD4I CCNGG 2 cut(s) 416, 597
StyI CCWWGG 2 cut(s) 408, 603
TaqI TCGA 1 cut(s) 429
TasI AATT 3 cut(s) 120, 156, 246
TfiI GAWTC 1 cut(s) 477
Tru1I TTAA 1 cut(s) 254
Tru9I TTAA 1 cut(s) 254
TseFI GTSAC 1 cut(s) 299
TseI GCWGC 2 cut(s) 269, 635
Tsp45I GTSAC 1 cut(s) 299
TspDTI ATGAA 1 cut(s) 191
Van91I CCANNNNNTGG 1 cut(s) 326
VpaK11BI GGWCC 1 cut(s) 572
XbaI TCTAGA 1 cut(s) 151
XceI RCATGY 1 cut(s) 511
XspI CTAG 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.