AT5G52960

Protein of unknown function (DUF3143)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010374
Physical Location & Seq
Forward (+)
21477116 .. 21478534
1419 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G52960.1

Sequence Viewer

Length: 513 bp
ATGAACTGTGTTTCTGCATTTGAATTTGTTCCTTCAAGTGCTCTATTTCCATGTGCACAATCATCAATCCCTGCGAAATTTCTTCACATTTTGAGCTCAAAAGCCTCGATTTCTCCCAGATGTCGTAGTTTCGCTTCTTCTTCTTCTTCCACAATTCGGAGGAAGCCTCTAACTCTAGTATCTTCAAAATCTTCAGACGCGGAGGAGGTGTCAGATACGGAGGACGAATGGCTGAAGAAGCTACCAGAGAAGAACAAGCCACTGTATTCGCATAGCTTACCTTGTATCGAAGCGTGGCTGAGGAAATTAGGGTTTTACCAAAGTAAAGACGATAGAGCTGTTTGGTTGATTCAGAAACCTGATTGGCATGCTCAACTATCTCTTGATGTCACTGATCTCTGCATTAGGTACATGAAAAGTGGGCCGGGAAATCTAGAGAGAGACATGGAAAGAAGGTTTAGCTATGCGTTGAGTAGAGAAGATACCGAGAATGCCATACTTGGAGGACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3143

Protein Analysis

170

Amino Acids

19.16

Weight (kDa)

8.27

Isoelectric Point (pI)

62.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3143 PF11341 103 - 169 8.8e-22 Protein of unknown function (DUF3143)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 200
AciI CCGC 1 cut(s) 200
AcsI RAATTY 2 cut(s) 23, 77
AcuI CTGAAG 2 cut(s) 177, 254
AfaI GTAC 1 cut(s) 410
AfiI CCNNNNNNNGG 1 cut(s) 156
AgsI TTSAA 3 cut(s) 23, 36, 186
AluBI AGCT 5 cut(s) 96, 241, 276, 338, 462
AluI AGCT 5 cut(s) 96, 241, 276, 338, 462
Alw21I GWGCWC 3 cut(s) 43, 58, 98
Alw26I GTCTC 1 cut(s) 435
Alw44I GTGCAC 1 cut(s) 54
AoxI GGCC 1 cut(s) 422
ApaLI GTGCAC 1 cut(s) 54
ApoI RAATTY 2 cut(s) 23, 77
Asp700I GAANNNNTTC 1 cut(s) 27
AspS9I GGNCC 2 cut(s) 422, 506
AsuC2I CCSGG 1 cut(s) 426
AvaII GGWCC 1 cut(s) 506
BaeGI GKGCMC 1 cut(s) 58
BaeI ACNNNNGTAYC 2 cut(s) 162, 195
BanII GRGCYC 1 cut(s) 98
BarI GAAGNNNNNNTAC 2 cut(s) 118, 150
Bbv12I GWGCWC 3 cut(s) 43, 58, 98
BbvCI CCTCAGC 1 cut(s) 299
BcnI CCSGG 1 cut(s) 426
BcoDI GTCTC 1 cut(s) 435
BfaI CTAG 2 cut(s) 176, 434
Bme1390I CCNGG 1 cut(s) 426
Bme18I GGWCC 1 cut(s) 506
BmgT120I GGNCC 2 cut(s) 422, 506
BmrFI CCNGG 1 cut(s) 426
BplI GAGNNNNNCTC 2 cut(s) 151, 183
Bpu10I CCTNAGC 1 cut(s) 299
BpuMI CCSGG 1 cut(s) 426
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 1 cut(s) 156
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMII CTCAG 1 cut(s) 290
BseRI GAGGAG 1 cut(s) 218
BseSI GKGCMC 1 cut(s) 58
Bsh1236I CGCG 1 cut(s) 200
BshFI GGCC 1 cut(s) 424
BsiHKAI GWGCWC 3 cut(s) 43, 58, 98
BsiSI CCGG 1 cut(s) 425
BslI CCNNNNNNNGG 1 cut(s) 156
BsmAI GTCTC 1 cut(s) 435
BsmI GAATGC 1 cut(s) 496
BsnI GGCC 1 cut(s) 424
Bsp1286I GDGCHC 3 cut(s) 43, 58, 98
Bsp143I GATC 1 cut(s) 394
BspACI CCGC 1 cut(s) 200
BspANI GGCC 1 cut(s) 424
BspCNI CTCAG 1 cut(s) 291
BspFNI CGCG 1 cut(s) 200
BssMI GATC 1 cut(s) 394
Bst4CI ACNGT 2 cut(s) 8, 264
BstC8I GCNNGC 1 cut(s) 369
BstDEI CTNAG 1 cut(s) 299
BstFNI CGCG 1 cut(s) 200
BstKTI GATC 1 cut(s) 397
BstMAI GTCTC 1 cut(s) 435
BstMBI GATC 1 cut(s) 394
BstMWI GCNNNNNNNGC 1 cut(s) 238
BstNSI RCATGY 1 cut(s) 371
BstSCI CCNGG 1 cut(s) 424
BstSLI GKGCMC 1 cut(s) 58
BstUI CGCG 1 cut(s) 200
BsuRI GGCC 1 cut(s) 424
BtsIMutI CAGTG 2 cut(s) 260, 390
Cac8I GCNNGC 1 cut(s) 369
Cfr13I GGNCC 2 cut(s) 422, 506
CseI GACGC 1 cut(s) 206
Csp6I GTAC 1 cut(s) 409
CviAII CATG 4 cut(s) 51, 368, 412, 445
CviQI GTAC 1 cut(s) 409
DdeI CTNAG 1 cut(s) 299
DpnI GATC 1 cut(s) 396
DpnII GATC 1 cut(s) 394
Ecl136II GAGCTC 1 cut(s) 96
Eco24I GRGCYC 1 cut(s) 98
Eco47I GGWCC 1 cut(s) 506
Eco53kI GAGCTC 1 cut(s) 96
Eco57I CTGAAG 2 cut(s) 177, 254
EcoICRI GAGCTC 1 cut(s) 96
EcoO109I RGGNCCY 1 cut(s) 506
EcoT38I GRGCYC 1 cut(s) 98
FaeI CATG 4 cut(s) 54, 371, 415, 448
FaiI YATR 7 cut(s) 52, 273, 369, 413, 446, 465, 497
FatI CATG 4 cut(s) 50, 367, 411, 444
FriOI GRGCYC 1 cut(s) 98
FspBI CTAG 2 cut(s) 176, 434
HaeIII GGCC 1 cut(s) 424
HapII CCGG 1 cut(s) 425
HgaI GACGC 1 cut(s) 206
Hin1II CATG 4 cut(s) 54, 371, 415, 448
HinfI GANTC 1 cut(s) 349
HpaII CCGG 1 cut(s) 425
Hpy166II GTNNAC 1 cut(s) 56
Hpy188I TCNGA 4 cut(s) 159, 196, 214, 354
Hpy188III TCNNGA 2 cut(s) 383, 434
Hpy8I GTNNAC 1 cut(s) 56
HpyAV CCTTC 2 cut(s) 42, 447
HpyCH4III ACNGT 2 cut(s) 8, 264
HpyCH4V TGCA 3 cut(s) 17, 56, 402
HpyF10VI GCNNNNNNNGC 1 cut(s) 238
HpyF3I CTNAG 1 cut(s) 299
Hsp92II CATG 4 cut(s) 54, 371, 415, 448
Kzo9I GATC 1 cut(s) 394
LpnPI CCDG 5 cut(s) 84, 130, 258, 372, 438
MaeI CTAG 2 cut(s) 176, 434
MaeIII GTNAC 1 cut(s) 388
MalI GATC 1 cut(s) 396
MboI GATC 1 cut(s) 394
MhlI GDGCHC 3 cut(s) 43, 58, 98
MluCI AATT 4 cut(s) 23, 77, 153, 305
MnlI CCTC 8 cut(s) 115, 153, 177, 196, 199, 214, 294, 497
MroXI GAANNNNTTC 1 cut(s) 27
MseI TTAA 1 cut(s) 511
MspI CCGG 1 cut(s) 425
MspR9I CCNGG 1 cut(s) 426
Mva1269I GAATGC 1 cut(s) 496
MvnI CGCG 1 cut(s) 200
MwoI GCNNNNNNNGC 1 cut(s) 238
NciI CCSGG 1 cut(s) 426
NdeII GATC 1 cut(s) 394
NlaIII CATG 4 cut(s) 54, 371, 415, 448
NmuCI GTSAC 1 cut(s) 388
NspI RCATGY 1 cut(s) 371
PaeI GCATGC 1 cut(s) 371
PctI GAATGC 1 cut(s) 496
PdmI GAANNNNTTC 1 cut(s) 27
PfeI GAWTC 1 cut(s) 349
PpuMI RGGWCCY 1 cut(s) 506
Psp124BI GAGCTC 1 cut(s) 98
Psp5II RGGWCCY 1 cut(s) 506
PspPI GGNCC 2 cut(s) 422, 506
PspPPI RGGWCCY 1 cut(s) 506
RsaI GTAC 1 cut(s) 410
RsaNI GTAC 1 cut(s) 409
SacI GAGCTC 1 cut(s) 98
SaqAI TTAA 1 cut(s) 511
Sau3AI GATC 1 cut(s) 394
Sau96I GGNCC 2 cut(s) 422, 506
ScrFI CCNGG 1 cut(s) 426
SduI GDGCHC 3 cut(s) 43, 58, 98
SinI GGWCC 1 cut(s) 506
SphI GCATGC 1 cut(s) 371
Sse9I AATT 4 cut(s) 23, 77, 153, 305
SsiI CCGC 1 cut(s) 200
SspMI CTAG 2 cut(s) 176, 434
SstI GAGCTC 1 cut(s) 98
StyD4I CCNGG 1 cut(s) 424
TaaI ACNGT 2 cut(s) 8, 264
TaqI TCGA 2 cut(s) 107, 288
TasI AATT 4 cut(s) 23, 77, 153, 305
TfiI GAWTC 1 cut(s) 349
Tru1I TTAA 1 cut(s) 511
Tru9I TTAA 1 cut(s) 511
TscAI CASTG 2 cut(s) 267, 397
TseFI GTSAC 1 cut(s) 388
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 2 cut(s) 17, 428
TspGWI ACGGA 1 cut(s) 233
TspRI CASTG 2 cut(s) 267, 397
VneI GTGCAC 1 cut(s) 54
VpaK11BI GGWCC 1 cut(s) 506
XapI RAATTY 2 cut(s) 23, 77
XbaI TCTAGA 1 cut(s) 433
XceI RCATGY 1 cut(s) 371
XmnI GAANNNNTTC 1 cut(s) 27
XspI CTAG 2 cut(s) 176, 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.