Rh1DG426100

Protein of unknown function (DUF3143)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0010374
Physical Location & Seq
Forward (+)
63921122 .. 63923009
1888 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG426100.1

Sequence Viewer

Length: 471 bp
ATGTCGACCGTCTTCTTCTCCGTTTCGAATTTCATCTTCTTCTCAAATCCAAAATACTCTGTTCTCTATTCAATTTCTCCTAAAGCGACGCCGTTTAGGCTCGTTCGGTCTCCGAGAAGGCTCGCGACTCCGGTATTTTCGAAGTCGTCGGAGGCGGAGGAGGTCTCGGCAGCGCCGGAGGACGAGTGGCTGAAGAGGCTACCGGACAAGAAGAAGCCTCTGTACTCTCACAGTCTTCCTTGCATTGAGGCCTGGCTGAGAAGCTTGGGGTTTTTCCAGAGCAGAGAGGACCGGGCGGTTTGGCTGATCGAAAAGCGCGAGTGGCATGCCCAGCTCTCTCTCGACGTCACCGATCTCTATGTCAGGTATCTAAAGACCGGACCTGGAAATCTTGAAAAGGATATGGAGAGGAGGTTTAGCTATGCACTGAGCAGAGAGGACATTGAAAATGCAGTGCTTGGAGGTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3143

Protein Analysis

156

Amino Acids

18.07

Weight (kDa)

9.01

Isoelectric Point (pI)

63.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3143 PF11341 89 - 155 8e-23 Protein of unknown function (DUF3143)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 348
AccI GTMKAC 1 cut(s) 5
AccII CGCG 2 cut(s) 125, 318
AciI CCGC 2 cut(s) 155, 296
AcsI RAATTY 1 cut(s) 28
AcuI CTGAAG 1 cut(s) 212
AcyI GRCGYC 2 cut(s) 89, 345
AfaI GTAC 1 cut(s) 224
AgsI TTSAA 3 cut(s) 72, 395, 446
AjnI CCWGG 2 cut(s) 251, 382
AluBI AGCT 3 cut(s) 264, 334, 420
AluI AGCT 3 cut(s) 264, 334, 420
Alw26I GTCTC 2 cut(s) 114, 169
AoxI GGCC 1 cut(s) 249
ApeKI GCWGC 1 cut(s) 170
ApoI RAATTY 1 cut(s) 28
AspLEI GCGC 2 cut(s) 175, 318
AspS9I GGNCC 3 cut(s) 289, 380, 464
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 340
AsuII TTCGAA 2 cut(s) 26, 140
AvaII GGWCC 3 cut(s) 289, 380, 464
BarI GAAGNNNNNNTAC 2 cut(s) 206, 238
BbsI GAAGAC 2 cut(s) 4, 227
BbvI GCAGC 1 cut(s) 182
BceAI ACGGC 1 cut(s) 76
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BciT130I CCWGG 2 cut(s) 253, 384
BcnI CCSGG 1 cut(s) 293
BcoDI GTCTC 2 cut(s) 114, 169
BfoI RGCGCY 1 cut(s) 176
BglI GCCNNNNNGGC 1 cut(s) 97
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
Bme1390I CCNGG 3 cut(s) 253, 293, 384
Bme18I GGWCC 3 cut(s) 289, 380, 464
BmgT120I GGNCC 3 cut(s) 289, 380, 464
BmiI GGNNCC 1 cut(s) 466
BmrFI CCNGG 3 cut(s) 253, 293, 384
BpiI GAAGAC 2 cut(s) 4, 227
BplI GAGNNNNNCTC 2 cut(s) 149, 181
Bpu14I TTCGAA 2 cut(s) 26, 140
BpuMI CCSGG 1 cut(s) 293
BsaHI GRCGYC 2 cut(s) 89, 345
BsaI GGTCTC 2 cut(s) 114, 169
BsaWI WCCGGW 3 cut(s) 130, 202, 377
BsaXI ACNNNNNCTCC 4 cut(s) 170, 200, 398, 428
BseBI CCWGG 2 cut(s) 253, 384
BseMII CTCAG 2 cut(s) 248, 419
BseRI GAGGAG 2 cut(s) 173, 424
BseXI GCAGC 1 cut(s) 182
BseYI CCCAGC 1 cut(s) 330
Bsh1236I CGCG 2 cut(s) 125, 318
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 1 cut(s) 251
BsiEI CGRYCG 1 cut(s) 9
BsiSI CCGG 5 cut(s) 131, 176, 203, 292, 378
BslFI GGGAC 1 cut(s) 450
BsmAI GTCTC 2 cut(s) 114, 169
BsmFI GGGAC 1 cut(s) 450
BsnI GGCC 1 cut(s) 251
Bso31I GGTCTC 2 cut(s) 114, 169
Bsp119I TTCGAA 2 cut(s) 26, 140
Bsp143I GATC 2 cut(s) 306, 352
Bsp68I TCGCGA 1 cut(s) 125
BspACI CCGC 2 cut(s) 155, 296
BspANI GGCC 1 cut(s) 251
BspCNI CTCAG 2 cut(s) 249, 420
BspFNI CGCG 2 cut(s) 125, 318
BspLI GGNNCC 1 cut(s) 466
BspT104I TTCGAA 2 cut(s) 26, 140
BspTNI GGTCTC 2 cut(s) 114, 169
BssMI GATC 2 cut(s) 306, 352
BssNI GRCGYC 2 cut(s) 89, 345
Bst2UI CCWGG 2 cut(s) 253, 384
Bst4CI ACNGT 2 cut(s) 10, 233
Bst6I CTCTTC 1 cut(s) 188
BstACI GRCGYC 2 cut(s) 89, 345
BstBI TTCGAA 2 cut(s) 26, 140
BstC8I GCNNGC 2 cut(s) 123, 327
BstDEI CTNAG 2 cut(s) 257, 428
BstFNI CGCG 2 cut(s) 125, 318
BstH2I RGCGCY 1 cut(s) 176
BstHHI GCGC 2 cut(s) 175, 318
BstKTI GATC 2 cut(s) 309, 355
BstMAI GTCTC 2 cut(s) 114, 169
BstMBI GATC 2 cut(s) 306, 352
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 4 cut(s) 97, 196, 322, 331
BstNI CCWGG 2 cut(s) 253, 384
BstNSI RCATGY 1 cut(s) 329
BstSCI CCNGG 3 cut(s) 251, 291, 382
BstUI CGCG 2 cut(s) 125, 318
BstV1I GCAGC 1 cut(s) 182
BstV2I GAAGAC 2 cut(s) 4, 227
BsuRI GGCC 1 cut(s) 251
BtsI GCAGTG 1 cut(s) 459
BtsIMutI CAGTG 2 cut(s) 425, 459
BtuMI TCGCGA 1 cut(s) 125
Cac8I GCNNGC 2 cut(s) 123, 327
CfoI GCGC 2 cut(s) 175, 318
Cfr13I GGNCC 3 cut(s) 289, 380, 464
CseI GACGC 1 cut(s) 97
Csp6I GTAC 1 cut(s) 223
CviAII CATG 1 cut(s) 326
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 2 cut(s) 257, 428
DpnI GATC 2 cut(s) 308, 354
DpnII GATC 2 cut(s) 306, 352
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
EciI GGCGGA 1 cut(s) 170
Eco147I AGGCCT 1 cut(s) 251
Eco31I GGTCTC 2 cut(s) 114, 169
Eco47I GGWCC 3 cut(s) 289, 380, 464
Eco57I CTGAAG 1 cut(s) 212
EcoO109I RGGNCCY 1 cut(s) 464
EcoRII CCWGG 2 cut(s) 251, 382
FaeI CATG 1 cut(s) 329
FaiI YATR 4 cut(s) 327, 360, 404, 423
FaqI GGGAC 1 cut(s) 450
FatI CATG 1 cut(s) 325
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 1 cut(s) 171
Fsp4HI GCNGC 1 cut(s) 171
GlaI GCGC 2 cut(s) 174, 317
GluI GCNGC 1 cut(s) 171
GsaI CCCAGC 1 cut(s) 334
HaeII RGCGCY 1 cut(s) 176
HaeIII GGCC 1 cut(s) 251
HapII CCGG 5 cut(s) 131, 176, 203, 292, 378
HgaI GACGC 1 cut(s) 97
HhaI GCGC 2 cut(s) 175, 318
Hin1I GRCGYC 2 cut(s) 89, 345
Hin1II CATG 1 cut(s) 329
Hin6I GCGC 2 cut(s) 173, 316
HinP1I GCGC 2 cut(s) 173, 316
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 262
HinfI GANTC 1 cut(s) 127
HpaII CCGG 5 cut(s) 131, 176, 203, 292, 378
HphI GGTGA 1 cut(s) 340
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 2 cut(s) 114, 151
Hpy188III TCNNGA 4 cut(s) 124, 277, 341, 392
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 3 cut(s) 91, 151, 347
HpyAV CCTTC 1 cut(s) 111
HpyCH4III ACNGT 2 cut(s) 10, 233
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 3 cut(s) 243, 425, 452
HpyF10VI GCNNNNNNNGC 4 cut(s) 97, 196, 322, 331
HpyF3I CTNAG 2 cut(s) 257, 428
HpySE526I ACGT 1 cut(s) 345
Hsp92I GRCGYC 2 cut(s) 89, 345
Hsp92II CATG 1 cut(s) 329
HspAI GCGC 2 cut(s) 173, 316
Kzo9I GATC 2 cut(s) 306, 352
Lsp1109I GCAGC 1 cut(s) 182
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 1 cut(s) 346
MalI GATC 2 cut(s) 308, 354
MboI GATC 2 cut(s) 306, 352
MboII GAAGA 7 cut(s) 4, 7, 28, 31, 205, 223, 227
MluCI AATT 2 cut(s) 28, 72
MlyI GAGTC 1 cut(s) 121
MmeI TCCRAC 1 cut(s) 129
MspI CCGG 5 cut(s) 131, 176, 203, 292, 378
MspR9I CCNGG 3 cut(s) 253, 293, 384
MvaI CCWGG 2 cut(s) 253, 384
MvnI CGCG 2 cut(s) 125, 318
MwoI GCNNNNNNNGC 4 cut(s) 97, 196, 322, 331
NciI CCSGG 1 cut(s) 293
NdeII GATC 2 cut(s) 306, 352
NlaIII CATG 1 cut(s) 329
NlaIV GGNNCC 1 cut(s) 466
NmeAIII GCCGAG 1 cut(s) 146
NmuCI GTSAC 1 cut(s) 346
NruI TCGCGA 1 cut(s) 125
NspI RCATGY 1 cut(s) 329
NspV TTCGAA 2 cut(s) 26, 140
PaeI GCATGC 1 cut(s) 329
PceI AGGCCT 1 cut(s) 251
PcsI WCGNNNNNNNCGW 2 cut(s) 315, 348
PkrI GCNGC 1 cut(s) 172
PleI GAGTC 1 cut(s) 121
PpsI GAGTC 1 cut(s) 121
PpuMI RGGWCCY 1 cut(s) 464
Psp5II RGGWCCY 1 cut(s) 464
Psp6I CCWGG 2 cut(s) 251, 382
PspFI CCCAGC 1 cut(s) 330
PspGI CCWGG 2 cut(s) 251, 382
PspN4I GGNNCC 1 cut(s) 466
PspPI GGNCC 3 cut(s) 289, 380, 464
PspPPI RGGWCCY 1 cut(s) 464
RruI TCGCGA 1 cut(s) 125
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
SalI GTCGAC 1 cut(s) 4
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 2 cut(s) 306, 352
Sau96I GGNCC 3 cut(s) 289, 380, 464
SchI GAGTC 1 cut(s) 121
ScrFI CCNGG 3 cut(s) 253, 293, 384
SetI ASST 9 cut(s) 165, 266, 336, 348, 368, 385, 416, 422, 466
SfuI TTCGAA 2 cut(s) 26, 140
SinI GGWCC 3 cut(s) 289, 380, 464
SphI GCATGC 1 cut(s) 329
Sse9I AATT 2 cut(s) 28, 72
SseBI AGGCCT 1 cut(s) 251
SsiI CCGC 2 cut(s) 155, 296
StuI AGGCCT 1 cut(s) 251
StyD4I CCNGG 3 cut(s) 251, 291, 382
TaaI ACNGT 2 cut(s) 10, 233
TaiI ACGT 1 cut(s) 348
TaqI TCGA 5 cut(s) 5, 26, 140, 309, 342
TaqII GACCGA 1 cut(s) 96
TasI AATT 2 cut(s) 28, 72
TatI WGTACW 1 cut(s) 222
TscAI CASTG 2 cut(s) 432, 459
TseFI GTSAC 1 cut(s) 346
TseI GCWGC 1 cut(s) 170
Tsp45I GTSAC 1 cut(s) 346
TspDTI ATGAA 1 cut(s) 22
TspGWI ACGGA 1 cut(s) 10
TspRI CASTG 2 cut(s) 432, 459
VpaK11BI GGWCC 3 cut(s) 289, 380, 464
XapI RAATTY 1 cut(s) 28
XceI RCATGY 1 cut(s) 329
XmiI GTMKAC 1 cut(s) 5
ZraI GACGTC 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.