RLG00000026357

Protein of unknown function (DUF3143)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0010374
Physical Location & Seq
Reverse (-)
2396822 .. 2398759
1938 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026357

Sequence Viewer

Length: 471 bp
ATGTCGACCGTCTTCTTCTCCGTTTCGAATTTCATCTTCTTCTCAAATCCAAACTACTCTGTTCGCTATTCAATTTCGCCTAAGGCGACGCCGTTTCGGCTCGTTCGGTCTTCGAGAAGGCTCGCGACTCCGGTGTTTTCGAAGTCGTCGGAGGCGGAGGAGGTCTCGGCGGCGCCGGAGGACGAGTGGCTGAAGAGGCTACCGGACAAGAAGAAGCCTTTGTACTCTCACAGTCTTCCTTGCATTGAGGCCTGGCTGAGAAGCTTGGGGTTTTTCCAGAGCAGAGAGGACCGCGCCGTTTGGCTGATCGAAAAGCCCGAGTGGCACGCCCAGCTCTCTCTCGACGTCACGGATCTCTATGTCAGGTATCTAAAGACCGGACCTGGAAATCTTGAAAAGGATATGGAGAGGAGGTTTAGCTATGCACTGAGCAGAGAGGACATCGAAAATGCAGTGCTTGGAGGTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3143

Protein Analysis

157

Amino Acids

18.03

Weight (kDa)

8.68

Isoelectric Point (pI)

61.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3143 PF11341 89 - 155 1.7e-23 Protein of unknown function (DUF3143)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 348
AccB1I GGYRCC 1 cut(s) 172
AccI GTMKAC 1 cut(s) 5
AccII CGCG 2 cut(s) 125, 294
AciI CCGC 3 cut(s) 155, 170, 292
AclWI GGATC 1 cut(s) 360
AcsI RAATTY 1 cut(s) 28
AcuI CTGAAG 1 cut(s) 212
AcyI GRCGYC 3 cut(s) 89, 173, 345
AfaI GTAC 1 cut(s) 224
AgsI TTSAA 2 cut(s) 72, 395
AjnI CCWGG 2 cut(s) 251, 382
AluBI AGCT 3 cut(s) 264, 334, 420
AluI AGCT 3 cut(s) 264, 334, 420
Alw26I GTCTC 1 cut(s) 169
AlwI GGATC 1 cut(s) 360
Ama87I CYCGRG 1 cut(s) 317
AoxI GGCC 1 cut(s) 249
ApoI RAATTY 1 cut(s) 28
AspLEI GCGC 2 cut(s) 175, 296
AspS9I GGNCC 3 cut(s) 289, 380, 464
AsuII TTCGAA 2 cut(s) 26, 140
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 3 cut(s) 289, 380, 464
AxyI CCTNAGG 1 cut(s) 81
BanI GGYRCC 1 cut(s) 172
BarI GAAGNNNNNNTAC 2 cut(s) 206, 238
BbsI GAAGAC 3 cut(s) 4, 102, 227
BceAI ACGGC 2 cut(s) 76, 281
BciT130I CCWGG 2 cut(s) 253, 384
BcoDI GTCTC 1 cut(s) 169
BfoI RGCGCY 1 cut(s) 176
BglI GCCNNNNNGGC 2 cut(s) 97, 322
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
Bme1390I CCNGG 2 cut(s) 253, 384
Bme18I GGWCC 3 cut(s) 289, 380, 464
BmeT110I CYCGRG 1 cut(s) 317
BmgT120I GGNCC 3 cut(s) 289, 380, 464
BmiI GGNNCC 2 cut(s) 174, 466
BmrFI CCNGG 2 cut(s) 253, 384
BpiI GAAGAC 3 cut(s) 4, 102, 227
BplI GAGNNNNNCTC 2 cut(s) 149, 181
Bpu14I TTCGAA 2 cut(s) 26, 140
BsaHI GRCGYC 3 cut(s) 89, 173, 345
BsaI GGTCTC 1 cut(s) 169
BsaWI WCCGGW 3 cut(s) 130, 202, 377
BsaXI ACNNNNNCTCC 4 cut(s) 170, 200, 398, 428
Bse21I CCTNAGG 1 cut(s) 81
BseBI CCWGG 2 cut(s) 253, 384
BseMII CTCAG 2 cut(s) 248, 419
BseRI GAGGAG 2 cut(s) 173, 424
BseYI CCCAGC 1 cut(s) 330
Bsh1236I CGCG 2 cut(s) 125, 294
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 1 cut(s) 251
BshNI GGYRCC 1 cut(s) 172
BsiEI CGRYCG 1 cut(s) 9
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 4 cut(s) 131, 176, 203, 378
BslFI GGGAC 1 cut(s) 450
BsmAI GTCTC 1 cut(s) 169
BsmFI GGGAC 1 cut(s) 450
BsnI GGCC 1 cut(s) 251
Bso31I GGTCTC 1 cut(s) 169
BsoBI CYCGRG 1 cut(s) 317
Bsp119I TTCGAA 2 cut(s) 26, 140
Bsp143I GATC 2 cut(s) 306, 352
Bsp68I TCGCGA 1 cut(s) 125
BspACI CCGC 3 cut(s) 155, 170, 292
BspANI GGCC 1 cut(s) 251
BspCNI CTCAG 2 cut(s) 249, 420
BspFNI CGCG 2 cut(s) 125, 294
BspLI GGNNCC 2 cut(s) 174, 466
BspPI GGATC 1 cut(s) 360
BspT104I TTCGAA 2 cut(s) 26, 140
BspT107I GGYRCC 1 cut(s) 172
BspTNI GGTCTC 1 cut(s) 169
BssMI GATC 2 cut(s) 306, 352
BssNI GRCGYC 3 cut(s) 89, 173, 345
Bst2UI CCWGG 2 cut(s) 253, 384
Bst4CI ACNGT 2 cut(s) 10, 233
Bst6I CTCTTC 1 cut(s) 188
BstACI GRCGYC 3 cut(s) 89, 173, 345
BstBI TTCGAA 2 cut(s) 26, 140
BstC8I GCNNGC 2 cut(s) 123, 327
BstDEI CTNAG 3 cut(s) 81, 257, 428
BstFNI CGCG 2 cut(s) 125, 294
BstH2I RGCGCY 1 cut(s) 176
BstHHI GCGC 2 cut(s) 175, 296
BstKTI GATC 2 cut(s) 309, 355
BstMAI GTCTC 1 cut(s) 169
BstMBI GATC 2 cut(s) 306, 352
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 4 cut(s) 97, 196, 322, 331
BstNI CCWGG 2 cut(s) 253, 384
BstSCI CCNGG 2 cut(s) 251, 382
BstUI CGCG 2 cut(s) 125, 294
BstV2I GAAGAC 3 cut(s) 4, 102, 227
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
Bsu36I CCTNAGG 1 cut(s) 81
BsuRI GGCC 1 cut(s) 251
BtsI GCAGTG 1 cut(s) 459
BtsIMutI CAGTG 2 cut(s) 425, 459
BtuMI TCGCGA 1 cut(s) 125
Cac8I GCNNGC 2 cut(s) 123, 327
CfoI GCGC 2 cut(s) 175, 296
Cfr13I GGNCC 3 cut(s) 289, 380, 464
CseI GACGC 1 cut(s) 97
Csp6I GTAC 1 cut(s) 223
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 3 cut(s) 81, 257, 428
DinI GGCGCC 1 cut(s) 174
DpnI GATC 2 cut(s) 308, 354
DpnII GATC 2 cut(s) 306, 352
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
EciI GGCGGA 1 cut(s) 170
Eco147I AGGCCT 1 cut(s) 251
Eco31I GGTCTC 1 cut(s) 169
Eco47I GGWCC 3 cut(s) 289, 380, 464
Eco57I CTGAAG 1 cut(s) 212
Eco81I CCTNAGG 1 cut(s) 81
Eco88I CYCGRG 1 cut(s) 317
EcoO109I RGGNCCY 1 cut(s) 464
EcoRII CCWGG 2 cut(s) 251, 382
EgeI GGCGCC 1 cut(s) 174
EheI GGCGCC 1 cut(s) 174
FaiI YATR 3 cut(s) 360, 404, 423
FaqI GGGAC 1 cut(s) 450
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 1 cut(s) 171
Fsp4HI GCNGC 1 cut(s) 171
GlaI GCGC 2 cut(s) 174, 295
GluI GCNGC 1 cut(s) 171
GsaI CCCAGC 1 cut(s) 334
HaeII RGCGCY 1 cut(s) 176
HaeIII GGCC 1 cut(s) 251
HapII CCGG 4 cut(s) 131, 176, 203, 378
HgaI GACGC 1 cut(s) 97
HhaI GCGC 2 cut(s) 175, 296
Hin1I GRCGYC 3 cut(s) 89, 173, 345
Hin6I GCGC 2 cut(s) 173, 294
HinP1I GCGC 2 cut(s) 173, 294
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 262
HinfI GANTC 1 cut(s) 127
HpaII CCGG 4 cut(s) 131, 176, 203, 378
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 1 cut(s) 151
Hpy188III TCNNGA 5 cut(s) 114, 124, 277, 341, 392
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 3 cut(s) 91, 151, 347
HpyAV CCTTC 1 cut(s) 111
HpyCH4III ACNGT 2 cut(s) 10, 233
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 3 cut(s) 243, 425, 452
HpyF10VI GCNNNNNNNGC 4 cut(s) 97, 196, 322, 331
HpyF3I CTNAG 3 cut(s) 81, 257, 428
HpySE526I ACGT 1 cut(s) 345
Hsp92I GRCGYC 3 cut(s) 89, 173, 345
HspAI GCGC 2 cut(s) 173, 294
KasI GGCGCC 1 cut(s) 172
Kzo9I GATC 2 cut(s) 306, 352
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 1 cut(s) 346
MalI GATC 2 cut(s) 308, 354
MboI GATC 2 cut(s) 306, 352
MboII GAAGA 8 cut(s) 4, 7, 28, 31, 102, 205, 223, 227
MflI RGATCY 1 cut(s) 352
MluCI AATT 2 cut(s) 28, 72
Mly113I GGCGCC 1 cut(s) 173
MlyI GAGTC 1 cut(s) 121
MmeI TCCRAC 1 cut(s) 129
MspI CCGG 4 cut(s) 131, 176, 203, 378
MspR9I CCNGG 2 cut(s) 253, 384
MvaI CCWGG 2 cut(s) 253, 384
MvnI CGCG 2 cut(s) 125, 294
MwoI GCNNNNNNNGC 4 cut(s) 97, 196, 322, 331
NarI GGCGCC 1 cut(s) 173
NdeII GATC 2 cut(s) 306, 352
NlaIV GGNNCC 2 cut(s) 174, 466
NmeAIII GCCGAG 1 cut(s) 146
NmuCI GTSAC 1 cut(s) 346
NruI TCGCGA 1 cut(s) 125
NspV TTCGAA 2 cut(s) 26, 140
PceI AGGCCT 1 cut(s) 251
PcsI WCGNNNNNNNCGW 2 cut(s) 83, 315
PkrI GCNGC 1 cut(s) 172
PleI GAGTC 1 cut(s) 121
PluTI GGCGCC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 121
PpuMI RGGWCCY 1 cut(s) 464
Psp5II RGGWCCY 1 cut(s) 464
Psp6I CCWGG 2 cut(s) 251, 382
PspFI CCCAGC 1 cut(s) 330
PspGI CCWGG 2 cut(s) 251, 382
PspN4I GGNNCC 2 cut(s) 174, 466
PspPI GGNCC 3 cut(s) 289, 380, 464
PspPPI RGGWCCY 1 cut(s) 464
PsuI RGATCY 1 cut(s) 352
RruI TCGCGA 1 cut(s) 125
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
SalI GTCGAC 1 cut(s) 4
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 2 cut(s) 306, 352
Sau96I GGNCC 3 cut(s) 289, 380, 464
SchI GAGTC 1 cut(s) 121
ScrFI CCNGG 2 cut(s) 253, 384
SetI ASST 9 cut(s) 165, 266, 336, 348, 368, 385, 416, 422, 466
SfoI GGCGCC 1 cut(s) 174
SfuI TTCGAA 2 cut(s) 26, 140
SinI GGWCC 3 cut(s) 289, 380, 464
Sse9I AATT 2 cut(s) 28, 72
SseBI AGGCCT 1 cut(s) 251
SsiI CCGC 3 cut(s) 155, 170, 292
SspDI GGCGCC 1 cut(s) 172
StuI AGGCCT 1 cut(s) 251
StyD4I CCNGG 2 cut(s) 251, 382
TaaI ACNGT 2 cut(s) 10, 233
TaiI ACGT 1 cut(s) 348
TaqI TCGA 7 cut(s) 5, 26, 113, 140, 309, 342, 444
TaqII GACCGA 1 cut(s) 96
TasI AATT 2 cut(s) 28, 72
TatI WGTACW 1 cut(s) 222
TauI GCSGC 1 cut(s) 173
TscAI CASTG 2 cut(s) 432, 459
TseFI GTSAC 1 cut(s) 346
Tsp45I GTSAC 1 cut(s) 346
TspDTI ATGAA 1 cut(s) 22
TspGWI ACGGA 2 cut(s) 10, 365
TspRI CASTG 2 cut(s) 432, 459
VpaK11BI GGWCC 3 cut(s) 289, 380, 464
XapI RAATTY 1 cut(s) 28
XmiI GTMKAC 1 cut(s) 5
ZraI GACGTC 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.