Rw1G038210

Protein of unknown function (DUF3143)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0010374
Physical Location & Seq
Forward (+)
65811391 .. 65813157
1767 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G038210.1

Sequence Viewer

Length: 414 bp
ATGTCGACCGTCTTCTTCTTCTTCGTTTCGAATTTCATCTTCTTCTCAAATCCAAAATACTCTGTTCGCTATTCAATTTCTCCTAAAGCGACGCCGTTTAGGCTCGTTCGGTCTCCGAGAAGGCTCGCGACTCCGGTGTTTTCGAAGTCGTCGGAGGCGCCGGAGGACGAGTGGCTGAAGAGGCTACCGGACAAGAAGAAGCCTTTCTTGGGGTTTTTCCAGAGCGGAGAGGACCGGGCCGTTTGGCTGATCGAAAAGCCCGAGTGGCATGCCCAGCTCTCTCTCGACGTTACCGATCTCTATGTCAGGTATCTGAAGACCGGACCTGGAAATCTTGAAAAGGATATGGAGAGGAGGTTTAGCTATGCACTGAGCAGAGACGACATCGGAAATGCAGTGCTTGGAGGTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3143

Protein Analysis

137

Amino Acids

15.88

Weight (kDa)

9.56

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3143 PF11341 70 - 136 4.9e-21 Protein of unknown function (DUF3143)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 157
AccBSI CCGCTC 1 cut(s) 225
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 128
AciI CCGC 1 cut(s) 225
AcsI RAATTY 1 cut(s) 31
AcuI CTGAAG 2 cut(s) 197, 335
AcyI GRCGYC 2 cut(s) 92, 158
AfiI CCNNNNNNNGG 1 cut(s) 209
AgsI TTSAA 2 cut(s) 75, 338
AjnI CCWGG 1 cut(s) 325
AjuI GAANNNNNNNTTGG 2 cut(s) 191, 223
AluBI AGCT 2 cut(s) 277, 363
AluI AGCT 2 cut(s) 277, 363
Alw26I GTCTC 2 cut(s) 117, 372
Ama87I CYCGRG 1 cut(s) 260
AoxI GGCC 1 cut(s) 237
ApoI RAATTY 1 cut(s) 31
Asp700I GAANNNNTTC 1 cut(s) 203
AspLEI GCGC 1 cut(s) 160
AspS9I GGNCC 4 cut(s) 232, 237, 323, 407
AsuC2I CCSGG 1 cut(s) 236
AsuII TTCGAA 2 cut(s) 29, 143
AvaI CYCGRG 1 cut(s) 260
AvaII GGWCC 3 cut(s) 232, 323, 407
BanI GGYRCC 1 cut(s) 157
BbsI GAAGAC 2 cut(s) 4, 323
BceAI ACGGC 2 cut(s) 79, 224
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 1 cut(s) 327
BcnI CCSGG 1 cut(s) 236
BcoDI GTCTC 2 cut(s) 117, 372
BfoI RGCGCY 1 cut(s) 161
BglI GCCNNNNNGGC 2 cut(s) 100, 265
Bme1390I CCNGG 2 cut(s) 236, 327
Bme18I GGWCC 3 cut(s) 232, 323, 407
BmeT110I CYCGRG 1 cut(s) 260
BmgT120I GGNCC 4 cut(s) 232, 237, 323, 407
BmiI GGNNCC 2 cut(s) 159, 409
BmrFI CCNGG 2 cut(s) 236, 327
BpiI GAAGAC 2 cut(s) 4, 323
Bpu14I TTCGAA 2 cut(s) 29, 143
BpuMI CCSGG 1 cut(s) 236
BsaHI GRCGYC 2 cut(s) 92, 158
BsaI GGTCTC 1 cut(s) 117
BsaWI WCCGGW 3 cut(s) 133, 187, 320
BsaXI ACNNNNNCTCC 4 cut(s) 155, 185, 341, 371
Bsc4I CCNNNNNNNGG 1 cut(s) 209
BseBI CCWGG 1 cut(s) 327
BseLI CCNNNNNNNGG 1 cut(s) 209
BseMII CTCAG 1 cut(s) 362
BseRI GAGGAG 1 cut(s) 367
BseYI CCCAGC 1 cut(s) 273
Bsh1236I CGCG 1 cut(s) 128
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 1 cut(s) 239
BshNI GGYRCC 1 cut(s) 157
BsiEI CGRYCG 1 cut(s) 9
BsiHKCI CYCGRG 1 cut(s) 260
BsiSI CCGG 5 cut(s) 134, 161, 188, 235, 321
BslFI GGGAC 1 cut(s) 393
BslI CCNNNNNNNGG 1 cut(s) 209
BsmAI GTCTC 2 cut(s) 117, 372
BsmBI CGTCTC 1 cut(s) 372
BsmFI GGGAC 1 cut(s) 393
BsnI GGCC 1 cut(s) 239
Bso31I GGTCTC 1 cut(s) 117
BsoBI CYCGRG 1 cut(s) 260
Bsp119I TTCGAA 2 cut(s) 29, 143
Bsp143I GATC 2 cut(s) 249, 295
Bsp68I TCGCGA 1 cut(s) 128
BspACI CCGC 1 cut(s) 225
BspANI GGCC 1 cut(s) 239
BspCNI CTCAG 1 cut(s) 363
BspFNI CGCG 1 cut(s) 128
BspLI GGNNCC 2 cut(s) 159, 409
BspT104I TTCGAA 2 cut(s) 29, 143
BspT107I GGYRCC 1 cut(s) 157
BspTNI GGTCTC 1 cut(s) 117
BsrBI CCGCTC 1 cut(s) 225
BssMI GATC 2 cut(s) 249, 295
BssNI GRCGYC 2 cut(s) 92, 158
Bst2UI CCWGG 1 cut(s) 327
Bst4CI ACNGT 1 cut(s) 10
Bst6I CTCTTC 1 cut(s) 173
BstACI GRCGYC 2 cut(s) 92, 158
BstBI TTCGAA 2 cut(s) 29, 143
BstC8I GCNNGC 2 cut(s) 126, 270
BstDEI CTNAG 1 cut(s) 371
BstFNI CGCG 1 cut(s) 128
BstH2I RGCGCY 1 cut(s) 161
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 2 cut(s) 252, 298
BstMAI GTCTC 2 cut(s) 117, 372
BstMBI GATC 2 cut(s) 249, 295
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 4 cut(s) 100, 181, 265, 274
BstNI CCWGG 1 cut(s) 327
BstNSI RCATGY 1 cut(s) 272
BstSCI CCNGG 2 cut(s) 234, 325
BstUI CGCG 1 cut(s) 128
BstV2I GAAGAC 2 cut(s) 4, 323
BsuRI GGCC 1 cut(s) 239
BtsI GCAGTG 1 cut(s) 402
BtsIMutI CAGTG 2 cut(s) 368, 402
BtuMI TCGCGA 1 cut(s) 128
Cac8I GCNNGC 2 cut(s) 126, 270
CfoI GCGC 1 cut(s) 160
Cfr13I GGNCC 4 cut(s) 232, 237, 323, 407
CseI GACGC 1 cut(s) 100
CviAII CATG 1 cut(s) 269
DdeI CTNAG 1 cut(s) 371
DinI GGCGCC 1 cut(s) 159
DpnI GATC 2 cut(s) 251, 297
DpnII GATC 2 cut(s) 249, 295
Eam1104I CTCTTC 1 cut(s) 173
EarI CTCTTC 1 cut(s) 173
Eco31I GGTCTC 1 cut(s) 117
Eco47I GGWCC 3 cut(s) 232, 323, 407
Eco57I CTGAAG 2 cut(s) 197, 335
Eco88I CYCGRG 1 cut(s) 260
EcoO109I RGGNCCY 1 cut(s) 407
EcoRII CCWGG 1 cut(s) 325
EgeI GGCGCC 1 cut(s) 159
EheI GGCGCC 1 cut(s) 159
Esp3I CGTCTC 1 cut(s) 372
FaeI CATG 1 cut(s) 272
FaiI YATR 4 cut(s) 270, 303, 347, 366
FalI AAGNNNNNCTT 2 cut(s) 191, 223
FaqI GGGAC 1 cut(s) 393
FatI CATG 1 cut(s) 268
FblI GTMKAC 1 cut(s) 5
GlaI GCGC 1 cut(s) 159
GsaI CCCAGC 1 cut(s) 277
HaeII RGCGCY 1 cut(s) 161
HaeIII GGCC 1 cut(s) 239
HapII CCGG 5 cut(s) 134, 161, 188, 235, 321
HgaI GACGC 1 cut(s) 100
HhaI GCGC 1 cut(s) 160
Hin1I GRCGYC 2 cut(s) 92, 158
Hin1II CATG 1 cut(s) 272
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 1 cut(s) 130
HpaII CCGG 5 cut(s) 134, 161, 188, 235, 321
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 4 cut(s) 117, 154, 315, 389
Hpy188III TCNNGA 4 cut(s) 127, 220, 284, 335
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 3 cut(s) 94, 154, 290
HpyAV CCTTC 1 cut(s) 114
HpyCH4III ACNGT 1 cut(s) 10
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 2 cut(s) 368, 395
HpyF10VI GCNNNNNNNGC 4 cut(s) 100, 181, 265, 274
HpyF3I CTNAG 1 cut(s) 371
HpySE526I ACGT 1 cut(s) 288
Hsp92I GRCGYC 2 cut(s) 92, 158
Hsp92II CATG 1 cut(s) 272
HspAI GCGC 1 cut(s) 158
KasI GGCGCC 1 cut(s) 157
Kzo9I GATC 2 cut(s) 249, 295
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 1 cut(s) 289
MalI GATC 2 cut(s) 251, 297
MbiI CCGCTC 1 cut(s) 225
MboI GATC 2 cut(s) 249, 295
MboII GAAGA 9 cut(s) 4, 7, 10, 13, 31, 34, 190, 208, 328
MluCI AATT 2 cut(s) 31, 75
Mly113I GGCGCC 1 cut(s) 158
MlyI GAGTC 1 cut(s) 124
MmeI TCCRAC 1 cut(s) 132
MnlI CCTC 7 cut(s) 148, 157, 174, 223, 345, 348, 398
MroXI GAANNNNTTC 1 cut(s) 203
MspI CCGG 5 cut(s) 134, 161, 188, 235, 321
MspR9I CCNGG 2 cut(s) 236, 327
MvaI CCWGG 1 cut(s) 327
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 4 cut(s) 100, 181, 265, 274
NarI GGCGCC 1 cut(s) 158
NciI CCSGG 1 cut(s) 236
NdeII GATC 2 cut(s) 249, 295
NlaIII CATG 1 cut(s) 272
NlaIV GGNNCC 2 cut(s) 159, 409
NruI TCGCGA 1 cut(s) 128
NspI RCATGY 1 cut(s) 272
NspV TTCGAA 2 cut(s) 29, 143
PaeI GCATGC 1 cut(s) 272
PcsI WCGNNNNNNNCGW 2 cut(s) 258, 291
PdmI GAANNNNTTC 1 cut(s) 203
PleI GAGTC 1 cut(s) 124
PluTI GGCGCC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 124
PpuMI RGGWCCY 1 cut(s) 407
Psp5II RGGWCCY 1 cut(s) 407
Psp6I CCWGG 1 cut(s) 325
PspFI CCCAGC 1 cut(s) 273
PspGI CCWGG 1 cut(s) 325
PspN4I GGNNCC 2 cut(s) 159, 409
PspPI GGNCC 4 cut(s) 232, 237, 323, 407
PspPPI RGGWCCY 1 cut(s) 407
RruI TCGCGA 1 cut(s) 128
SalI GTCGAC 1 cut(s) 4
Sau3AI GATC 2 cut(s) 249, 295
Sau96I GGNCC 4 cut(s) 232, 237, 323, 407
SchI GAGTC 1 cut(s) 124
ScrFI CCNGG 2 cut(s) 236, 327
SetI ASST 7 cut(s) 279, 291, 311, 328, 359, 365, 409
SfoI GGCGCC 1 cut(s) 159
SfuI TTCGAA 2 cut(s) 29, 143
SinI GGWCC 3 cut(s) 232, 323, 407
SphI GCATGC 1 cut(s) 272
Sse9I AATT 2 cut(s) 31, 75
SsiI CCGC 1 cut(s) 225
SspDI GGCGCC 1 cut(s) 157
StyD4I CCNGG 2 cut(s) 234, 325
TaaI ACNGT 1 cut(s) 10
TaiI ACGT 1 cut(s) 291
TaqI TCGA 5 cut(s) 5, 29, 143, 252, 285
TaqII GACCGA 1 cut(s) 99
TasI AATT 2 cut(s) 31, 75
TscAI CASTG 2 cut(s) 375, 402
TspDTI ATGAA 1 cut(s) 25
TspRI CASTG 2 cut(s) 375, 402
VpaK11BI GGWCC 3 cut(s) 232, 323, 407
XapI RAATTY 1 cut(s) 31
XceI RCATGY 1 cut(s) 272
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.