Rroxscaffold_4G00279920

Protein of unknown function (DUF3143)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0010374
Physical Location & Seq
Reverse (-)
2567260 .. 2569187
1928 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00279920.1

Sequence Viewer

Length: 459 bp
ATGTCGACCGTCTTCTTCTCCGTTTCGAATTTCATCTTCTTCTCAAATCCAAACTACTCTGTTCGCTATTCAATTTCTCCTAAAGCGACGCCGTTTCGGTTCTTTCGGTCTTCGAGAAGTCTCGCGACTCCGGTGTTTTCGAAGTCGTCGGAGGCGGAGGAGGTCTCAGCGGCGCCGGAGGACGAGTGGCTGAAGAGGCTACCGGACAAGAAGAAGCCTCTGTACTCTCACAGTCTTCCTTGCATTGAGGCCTGGCTGAGAAGCTTGGGGTTTTTCCAGAGCAGAGAGGACCGGGCCGTTTGGCTGATCGAAAAGCCCGAGTGGCATGCCCAGCTCTCTCTCGACGTCACAGGGTATCTAAAGACCGGACCTGGAAATCTTGAAAAGGATATGGAGAGGAGGTTTAGCTATGCACTGAGCAGAGAGGACATCGAAAATGCAGTGCTCGGAGGTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3143

Protein Analysis

152

Amino Acids

17.45

Weight (kDa)

7.84

Isoelectric Point (pI)

61.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3143 PF11341 89 - 151 1.6e-17 Protein of unknown function (DUF3143)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 348
AccB1I GGYRCC 1 cut(s) 172
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 125
AciI CCGC 2 cut(s) 155, 170
AcsI RAATTY 1 cut(s) 28
AcuI CTGAAG 1 cut(s) 212
AcyI GRCGYC 3 cut(s) 89, 173, 345
AfaI GTAC 1 cut(s) 224
AgsI TTSAA 2 cut(s) 72, 383
AjnI CCWGG 2 cut(s) 251, 370
AluBI AGCT 3 cut(s) 264, 334, 408
AluI AGCT 3 cut(s) 264, 334, 408
Alw21I GWGCWC 1 cut(s) 447
Alw26I GTCTC 2 cut(s) 125, 169
Ama87I CYCGRG 1 cut(s) 317
AoxI GGCC 2 cut(s) 249, 294
ApoI RAATTY 1 cut(s) 28
AspLEI GCGC 1 cut(s) 175
AspS9I GGNCC 4 cut(s) 289, 294, 368, 452
AsuC2I CCSGG 1 cut(s) 293
AsuII TTCGAA 2 cut(s) 26, 140
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 3 cut(s) 289, 368, 452
BanI GGYRCC 1 cut(s) 172
BarI GAAGNNNNNNTAC 2 cut(s) 206, 238
BbsI GAAGAC 3 cut(s) 4, 102, 227
Bbv12I GWGCWC 1 cut(s) 447
BceAI ACGGC 2 cut(s) 76, 281
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BciT130I CCWGG 2 cut(s) 253, 372
BcnI CCSGG 1 cut(s) 293
BcoDI GTCTC 2 cut(s) 125, 169
BfoI RGCGCY 1 cut(s) 176
BglI GCCNNNNNGGC 1 cut(s) 322
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
Bme1390I CCNGG 3 cut(s) 253, 293, 372
Bme18I GGWCC 3 cut(s) 289, 368, 452
BmeT110I CYCGRG 1 cut(s) 317
BmgT120I GGNCC 4 cut(s) 289, 294, 368, 452
BmiI GGNNCC 2 cut(s) 174, 454
BmrFI CCNGG 3 cut(s) 253, 293, 372
BpiI GAAGAC 3 cut(s) 4, 102, 227
BplI GAGNNNNNCTC 2 cut(s) 149, 181
Bpu14I TTCGAA 2 cut(s) 26, 140
BpuMI CCSGG 1 cut(s) 293
BsaHI GRCGYC 3 cut(s) 89, 173, 345
BsaI GGTCTC 1 cut(s) 169
BsaWI WCCGGW 3 cut(s) 130, 202, 365
BsaXI ACNNNNNCTCC 4 cut(s) 170, 200, 386, 416
BseBI CCWGG 2 cut(s) 253, 372
BseMII CTCAG 3 cut(s) 180, 248, 407
BseRI GAGGAG 2 cut(s) 173, 412
BseYI CCCAGC 1 cut(s) 330
Bsh1236I CGCG 1 cut(s) 125
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 2 cut(s) 251, 296
BshNI GGYRCC 1 cut(s) 172
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 447
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 5 cut(s) 131, 176, 203, 292, 366
BslFI GGGAC 1 cut(s) 438
BsmAI GTCTC 2 cut(s) 125, 169
BsmFI GGGAC 1 cut(s) 438
BsnI GGCC 2 cut(s) 251, 296
Bso31I GGTCTC 1 cut(s) 169
BsoBI CYCGRG 1 cut(s) 317
Bsp119I TTCGAA 2 cut(s) 26, 140
Bsp1286I GDGCHC 1 cut(s) 447
Bsp143I GATC 1 cut(s) 306
Bsp68I TCGCGA 1 cut(s) 125
BspACI CCGC 2 cut(s) 155, 170
BspANI GGCC 2 cut(s) 251, 296
BspCNI CTCAG 3 cut(s) 179, 249, 408
BspFNI CGCG 1 cut(s) 125
BspLI GGNNCC 2 cut(s) 174, 454
BspT104I TTCGAA 2 cut(s) 26, 140
BspT107I GGYRCC 1 cut(s) 172
BspTNI GGTCTC 1 cut(s) 169
BssMI GATC 1 cut(s) 306
BssNI GRCGYC 3 cut(s) 89, 173, 345
Bst2UI CCWGG 2 cut(s) 253, 372
Bst4CI ACNGT 2 cut(s) 10, 233
Bst6I CTCTTC 1 cut(s) 188
BstACI GRCGYC 3 cut(s) 89, 173, 345
BstBI TTCGAA 2 cut(s) 26, 140
BstC8I GCNNGC 1 cut(s) 327
BstDEI CTNAG 3 cut(s) 166, 257, 416
BstFNI CGCG 1 cut(s) 125
BstH2I RGCGCY 1 cut(s) 176
BstHHI GCGC 1 cut(s) 175
BstKTI GATC 1 cut(s) 309
BstMAI GTCTC 2 cut(s) 125, 169
BstMBI GATC 1 cut(s) 306
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 3 cut(s) 196, 322, 331
BstNI CCWGG 2 cut(s) 253, 372
BstNSI RCATGY 1 cut(s) 329
BstSCI CCNGG 3 cut(s) 251, 291, 370
BstUI CGCG 1 cut(s) 125
BstV2I GAAGAC 3 cut(s) 4, 102, 227
BsuRI GGCC 2 cut(s) 251, 296
BtsI GCAGTG 1 cut(s) 447
BtsIMutI CAGTG 2 cut(s) 413, 447
BtuMI TCGCGA 1 cut(s) 125
Cac8I GCNNGC 1 cut(s) 327
CfoI GCGC 1 cut(s) 175
Cfr13I GGNCC 4 cut(s) 289, 294, 368, 452
CseI GACGC 1 cut(s) 97
Csp6I GTAC 1 cut(s) 223
CviAII CATG 1 cut(s) 326
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 3 cut(s) 166, 257, 416
DinI GGCGCC 1 cut(s) 174
DpnI GATC 1 cut(s) 308
DpnII GATC 1 cut(s) 306
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
EciI GGCGGA 1 cut(s) 170
Eco147I AGGCCT 1 cut(s) 251
Eco31I GGTCTC 1 cut(s) 169
Eco47I GGWCC 3 cut(s) 289, 368, 452
Eco57I CTGAAG 1 cut(s) 212
Eco88I CYCGRG 1 cut(s) 317
EcoO109I RGGNCCY 1 cut(s) 452
EcoRII CCWGG 2 cut(s) 251, 370
EgeI GGCGCC 1 cut(s) 174
EheI GGCGCC 1 cut(s) 174
FaeI CATG 1 cut(s) 329
FaiI YATR 3 cut(s) 327, 392, 411
FaqI GGGAC 1 cut(s) 438
FatI CATG 1 cut(s) 325
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 1 cut(s) 171
Fsp4HI GCNGC 1 cut(s) 171
GlaI GCGC 1 cut(s) 174
GluI GCNGC 1 cut(s) 171
GsaI CCCAGC 1 cut(s) 334
HaeII RGCGCY 1 cut(s) 176
HaeIII GGCC 2 cut(s) 251, 296
HapII CCGG 5 cut(s) 131, 176, 203, 292, 366
HgaI GACGC 1 cut(s) 97
HhaI GCGC 1 cut(s) 175
Hin1I GRCGYC 3 cut(s) 89, 173, 345
Hin1II CATG 1 cut(s) 329
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 262
HinfI GANTC 1 cut(s) 127
HpaII CCGG 5 cut(s) 131, 176, 203, 292, 366
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 2 cut(s) 151, 449
Hpy188III TCNNGA 5 cut(s) 114, 124, 277, 341, 380
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 3 cut(s) 91, 151, 347
HpyCH4III ACNGT 2 cut(s) 10, 233
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 3 cut(s) 243, 413, 440
HpyF10VI GCNNNNNNNGC 3 cut(s) 196, 322, 331
HpyF3I CTNAG 3 cut(s) 166, 257, 416
HpySE526I ACGT 1 cut(s) 345
Hsp92I GRCGYC 3 cut(s) 89, 173, 345
Hsp92II CATG 1 cut(s) 329
HspAI GCGC 1 cut(s) 173
KasI GGCGCC 1 cut(s) 172
Kzo9I GATC 1 cut(s) 306
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 1 cut(s) 346
MalI GATC 1 cut(s) 308
MboI GATC 1 cut(s) 306
MboII GAAGA 8 cut(s) 4, 7, 28, 31, 102, 205, 223, 227
MhlI GDGCHC 1 cut(s) 447
MluCI AATT 2 cut(s) 28, 72
Mly113I GGCGCC 1 cut(s) 173
MlyI GAGTC 1 cut(s) 121
MmeI TCCRAC 1 cut(s) 129
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 5 cut(s) 131, 176, 203, 292, 366
MspR9I CCNGG 3 cut(s) 253, 293, 372
MvaI CCWGG 2 cut(s) 253, 372
MvnI CGCG 1 cut(s) 125
MwoI GCNNNNNNNGC 3 cut(s) 196, 322, 331
NarI GGCGCC 1 cut(s) 173
NciI CCSGG 1 cut(s) 293
NdeII GATC 1 cut(s) 306
NlaIII CATG 1 cut(s) 329
NlaIV GGNNCC 2 cut(s) 174, 454
NmuCI GTSAC 1 cut(s) 346
NruI TCGCGA 1 cut(s) 125
NspI RCATGY 1 cut(s) 329
NspV TTCGAA 2 cut(s) 26, 140
PaeI GCATGC 1 cut(s) 329
PceI AGGCCT 1 cut(s) 251
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PkrI GCNGC 1 cut(s) 172
PleI GAGTC 1 cut(s) 121
PluTI GGCGCC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 121
PpuMI RGGWCCY 1 cut(s) 452
Psp5II RGGWCCY 1 cut(s) 452
Psp6I CCWGG 2 cut(s) 251, 370
PspFI CCCAGC 1 cut(s) 330
PspGI CCWGG 2 cut(s) 251, 370
PspN4I GGNNCC 2 cut(s) 174, 454
PspPI GGNCC 4 cut(s) 289, 294, 368, 452
PspPPI RGGWCCY 1 cut(s) 452
RruI TCGCGA 1 cut(s) 125
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
SalI GTCGAC 1 cut(s) 4
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 1 cut(s) 306
Sau96I GGNCC 4 cut(s) 289, 294, 368, 452
SchI GAGTC 1 cut(s) 121
ScrFI CCNGG 3 cut(s) 253, 293, 372
SduI GDGCHC 1 cut(s) 447
SetI ASST 8 cut(s) 165, 266, 336, 348, 373, 404, 410, 454
SfoI GGCGCC 1 cut(s) 174
SfuI TTCGAA 2 cut(s) 26, 140
SinI GGWCC 3 cut(s) 289, 368, 452
SphI GCATGC 1 cut(s) 329
Sse9I AATT 2 cut(s) 28, 72
SseBI AGGCCT 1 cut(s) 251
SsiI CCGC 2 cut(s) 155, 170
SspDI GGCGCC 1 cut(s) 172
StuI AGGCCT 1 cut(s) 251
StyD4I CCNGG 3 cut(s) 251, 291, 370
TaaI ACNGT 2 cut(s) 10, 233
TaiI ACGT 1 cut(s) 348
TaqI TCGA 7 cut(s) 5, 26, 113, 140, 309, 342, 432
TaqII GACCGA 1 cut(s) 96
TasI AATT 2 cut(s) 28, 72
TatI WGTACW 1 cut(s) 222
TauI GCSGC 1 cut(s) 173
TscAI CASTG 2 cut(s) 420, 447
TseFI GTSAC 1 cut(s) 346
Tsp45I GTSAC 1 cut(s) 346
TspDTI ATGAA 1 cut(s) 22
TspGWI ACGGA 1 cut(s) 10
TspRI CASTG 2 cut(s) 420, 447
VpaK11BI GGWCC 3 cut(s) 289, 368, 452
XapI RAATTY 1 cut(s) 28
XceI RCATGY 1 cut(s) 329
XmiI GTMKAC 1 cut(s) 5
ZraI GACGTC 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.