RLG00000026361

Protein of unknown function (DUF3143)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0010374
Physical Location & Seq
Reverse (-)
2411374 .. 2413256
1883 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026361

Sequence Viewer

Length: 405 bp
ATGACGACCGTCTTCTTCTCCGTTTCGAATTTCATCTTCTTCTCAAATCCAAAATACTCTGTTCGCTATTCAATTTCGCCTAAAGCGACACCGTTTCGGCTCGGTCGGTCTTCGAGAAGGATCGCGATTCCGGTGTTTTCGAAGTCGTCGGAAGGCGGAGCGCCAGCCGGAGGACGAGTGGCTGAAGAGGCTACCGGACAAGAAGAAGCCTTTAGCAGAGAGGACCGTGCCGTTTGGCTGATCGAAAAGCCCGAGTGGCATGCCCAGCTCTCTCTCGACGTTACCGATCTCTTCTTCAGTTATCTAAAGACCGGACCTGGAAATCTTGTAAAGGATATGGAGAGGAGGTTTAGCTATGCACTGAGCAGAGAGGACATCGAAAATGCAGTGCTTGGAGGTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3143

Protein Analysis

135

Amino Acids

14.98

Weight (kDa)

8.01

Isoelectric Point (pI)

55.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3143 PF11341 72 - 133 1.3e-19 Protein of unknown function (DUF3143)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 125
AciI CCGC 1 cut(s) 156
AclWI GGATC 1 cut(s) 128
AcsI RAATTY 1 cut(s) 28
AcuI CTGAAG 2 cut(s) 204, 280
AfiI CCNNNNNNNGG 1 cut(s) 170
AgsI TTSAA 1 cut(s) 72
AjnI CCWGG 1 cut(s) 316
AluBI AGCT 2 cut(s) 268, 354
AluI AGCT 2 cut(s) 268, 354
AlwI GGATC 1 cut(s) 128
Ama87I CYCGRG 1 cut(s) 251
ApoI RAATTY 1 cut(s) 28
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 3 cut(s) 223, 314, 398
AsuII TTCGAA 2 cut(s) 26, 140
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 3 cut(s) 223, 314, 398
BbsI GAAGAC 2 cut(s) 4, 102
BceAI ACGGC 1 cut(s) 215
BcgI CGANNNNNNTGC 2 cut(s) 242, 276
BciT130I CCWGG 1 cut(s) 318
BfoI RGCGCY 1 cut(s) 164
BglI GCCNNNNNGGC 1 cut(s) 256
Bme1390I CCNGG 1 cut(s) 318
Bme18I GGWCC 3 cut(s) 223, 314, 398
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 3 cut(s) 223, 314, 398
BmiI GGNNCC 1 cut(s) 400
BmrFI CCNGG 1 cut(s) 318
BoxI GACNNNNGTC 1 cut(s) 8
BpiI GAAGAC 2 cut(s) 4, 102
Bpu14I TTCGAA 2 cut(s) 26, 140
BsaWI WCCGGW 3 cut(s) 130, 194, 311
BsaXI ACNNNNNCTCC 4 cut(s) 162, 192, 332, 362
Bsc4I CCNNNNNNNGG 1 cut(s) 170
BseBI CCWGG 1 cut(s) 318
BseLI CCNNNNNNNGG 1 cut(s) 170
BseMII CTCAG 1 cut(s) 353
BseRI GAGGAG 1 cut(s) 358
BseYI CCCAGC 1 cut(s) 264
Bsh1236I CGCG 1 cut(s) 125
Bsh1285I CGRYCG 2 cut(s) 9, 106
BsiEI CGRYCG 2 cut(s) 9, 106
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 4 cut(s) 131, 168, 195, 312
BslFI GGGAC 1 cut(s) 384
BslI CCNNNNNNNGG 1 cut(s) 170
BsmFI GGGAC 1 cut(s) 384
BsoBI CYCGRG 1 cut(s) 251
Bsp119I TTCGAA 2 cut(s) 26, 140
Bsp143I GATC 3 cut(s) 120, 240, 286
Bsp68I TCGCGA 1 cut(s) 125
BspACI CCGC 1 cut(s) 156
BspCNI CTCAG 1 cut(s) 354
BspFNI CGCG 1 cut(s) 125
BspLI GGNNCC 1 cut(s) 400
BspPI GGATC 1 cut(s) 128
BspT104I TTCGAA 2 cut(s) 26, 140
BssMI GATC 3 cut(s) 120, 240, 286
Bst2UI CCWGG 1 cut(s) 318
Bst4CI ACNGT 3 cut(s) 10, 93, 227
Bst6I CTCTTC 2 cut(s) 180, 296
BstBI TTCGAA 2 cut(s) 26, 140
BstC8I GCNNGC 2 cut(s) 165, 261
BstDEI CTNAG 1 cut(s) 362
BstFNI CGCG 1 cut(s) 125
BstH2I RGCGCY 1 cut(s) 164
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 3 cut(s) 123, 243, 289
BstMBI GATC 3 cut(s) 120, 240, 286
BstMCI CGRYCG 2 cut(s) 9, 106
BstMWI GCNNNNNNNGC 3 cut(s) 188, 256, 265
BstNI CCWGG 1 cut(s) 318
BstNSI RCATGY 1 cut(s) 263
BstPAI GACNNNNGTC 1 cut(s) 8
BstSCI CCNGG 1 cut(s) 316
BstUI CGCG 1 cut(s) 125
BstV2I GAAGAC 2 cut(s) 4, 102
BtsI GCAGTG 1 cut(s) 393
BtsIMutI CAGTG 2 cut(s) 359, 393
BtuMI TCGCGA 1 cut(s) 125
Cac8I GCNNGC 2 cut(s) 165, 261
CfoI GCGC 1 cut(s) 163
Cfr13I GGNCC 3 cut(s) 223, 314, 398
CviAII CATG 1 cut(s) 260
CviJI RGCY 9 cut(s) 100, 167, 182, 191, 209, 238, 250, 268, 354
CviKI_1 RGCY 9 cut(s) 100, 167, 182, 191, 209, 238, 250, 268, 354
DdeI CTNAG 1 cut(s) 362
DpnI GATC 3 cut(s) 122, 242, 288
DpnII GATC 3 cut(s) 120, 240, 286
Eam1104I CTCTTC 2 cut(s) 180, 296
EarI CTCTTC 2 cut(s) 180, 296
EciI GGCGGA 1 cut(s) 171
Eco47I GGWCC 3 cut(s) 223, 314, 398
Eco57I CTGAAG 2 cut(s) 204, 280
Eco88I CYCGRG 1 cut(s) 251
EcoO109I RGGNCCY 1 cut(s) 398
EcoRII CCWGG 1 cut(s) 316
FaeI CATG 1 cut(s) 263
FaiI YATR 3 cut(s) 261, 338, 357
FaqI GGGAC 1 cut(s) 384
FatI CATG 1 cut(s) 259
GlaI GCGC 1 cut(s) 162
GsaI CCCAGC 1 cut(s) 268
HaeII RGCGCY 1 cut(s) 164
HapII CCGG 4 cut(s) 131, 168, 195, 312
HhaI GCGC 1 cut(s) 163
Hin1II CATG 1 cut(s) 263
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HinfI GANTC 1 cut(s) 127
HpaII CCGG 4 cut(s) 131, 168, 195, 312
Hpy188I TCNGA 1 cut(s) 151
Hpy188III TCNNGA 3 cut(s) 114, 124, 275
Hpy99I CGWCG 2 cut(s) 151, 281
HpyAV CCTTC 2 cut(s) 111, 146
HpyCH4III ACNGT 3 cut(s) 10, 93, 227
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 2 cut(s) 359, 386
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 256, 265
HpyF3I CTNAG 1 cut(s) 362
HpySE526I ACGT 1 cut(s) 279
Hsp92II CATG 1 cut(s) 263
HspAI GCGC 1 cut(s) 161
Kzo9I GATC 3 cut(s) 120, 240, 286
LmnI GCTCC 1 cut(s) 158
LpnPI CCDG 8 cut(s) 144, 177, 181, 208, 278, 303, 325, 330
MaeII ACGT 1 cut(s) 279
MaeIII GTNAC 1 cut(s) 280
MalI GATC 3 cut(s) 122, 242, 288
MboI GATC 3 cut(s) 120, 240, 286
MboII GAAGA 9 cut(s) 4, 7, 28, 31, 102, 197, 215, 283, 286
MluCI AATT 2 cut(s) 28, 72
MmeI TCCRAC 1 cut(s) 129
MnlI CCTC 7 cut(s) 164, 181, 214, 336, 339, 364, 389
MspI CCGG 4 cut(s) 131, 168, 195, 312
MspR9I CCNGG 1 cut(s) 318
MvaI CCWGG 1 cut(s) 318
MvnI CGCG 1 cut(s) 125
MwoI GCNNNNNNNGC 3 cut(s) 188, 256, 265
NdeII GATC 3 cut(s) 120, 240, 286
NlaIII CATG 1 cut(s) 263
NlaIV GGNNCC 1 cut(s) 400
NruI TCGCGA 1 cut(s) 125
NspI RCATGY 1 cut(s) 263
NspV TTCGAA 2 cut(s) 26, 140
PaeI GCATGC 1 cut(s) 263
PcsI WCGNNNNNNNCGW 3 cut(s) 83, 249, 282
PfeI GAWTC 1 cut(s) 127
PpuMI RGGWCCY 1 cut(s) 398
PshAI GACNNNNGTC 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 398
Psp6I CCWGG 1 cut(s) 316
PspFI CCCAGC 1 cut(s) 264
PspGI CCWGG 1 cut(s) 316
PspN4I GGNNCC 1 cut(s) 400
PspPI GGNCC 3 cut(s) 223, 314, 398
PspPPI RGGWCCY 1 cut(s) 398
RruI TCGCGA 1 cut(s) 125
Sau3AI GATC 3 cut(s) 120, 240, 286
Sau96I GGNCC 3 cut(s) 223, 314, 398
ScrFI CCNGG 1 cut(s) 318
SetI ASST 6 cut(s) 270, 282, 319, 350, 356, 400
SfuI TTCGAA 2 cut(s) 26, 140
SinI GGWCC 3 cut(s) 223, 314, 398
SphI GCATGC 1 cut(s) 263
Sse9I AATT 2 cut(s) 28, 72
SsiI CCGC 1 cut(s) 156
StyD4I CCNGG 1 cut(s) 316
TaaI ACNGT 3 cut(s) 10, 93, 227
TaiI ACGT 1 cut(s) 282
TaqI TCGA 6 cut(s) 26, 113, 140, 243, 276, 378
TaqII GACCGA 2 cut(s) 92, 96
TasI AATT 2 cut(s) 28, 72
TfiI GAWTC 1 cut(s) 127
TscAI CASTG 2 cut(s) 366, 393
TspDTI ATGAA 1 cut(s) 22
TspGWI ACGGA 1 cut(s) 10
TspRI CASTG 2 cut(s) 366, 393
VpaK11BI GGWCC 3 cut(s) 223, 314, 398
XapI RAATTY 1 cut(s) 28
XceI RCATGY 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.