Rorug01G0415200

Protein of unknown function (DUF3143)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0010374
Physical Location & Seq
Forward (+)
51862839 .. 51866003
3165 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0415200.1

Sequence Viewer

Length: 1581 bp
ATGCCGTCGCCGTCGTCTCTCCCCGAACCCCCGACCTCCCCGCCGCCGCCGCCCCTAGACGCCGCCGCCTCCGACCCCCTACGCGAGGCCGAGGACCGCCTCCGCGACGCCATTGAGGAACTCCAGCGCCGCCAGCGCACCGCCGCAGCCCGTGGCTCCCAGCACCACCCCCCCTGCGACCACGCCAACGACGAGTCGTGCGTCGCCCACGCCATTGGGAACCTCTGCCAGAGCTTTCTCCTCTCCTATGGCGTCCGAGTTGGGATTGGGATTCTTCTTCGCGCGTTTAAGCTCGCTCGCAGACAATCCTACTCTTCCTTGCTCGATCTTAAGCAACTTGTCTCGGAAAAAGATCTGATTGTGAGAGAAGAAGCATGCCGAATTGGCTTATTTTTTGGTGGATTTAGTGGTTCTTACCATGCTATTAGATGCTTGTTGAGAAAGTACAGAAAGAAAGAGACACCATTGAATGCAATCTTAGCAGGTTCAATTGCGGGGTTGTCTATTTTAGCATTAGATGACTCAAACCGAAGGCGCACACTATCTCTCTACTTGTTGGCAAGGGTAGCACAGTCTGCTTATAATTCTGCAAAGTCTAAGAACAAGTTTCACTTTTGGGGAAGCCACTGGAGGCATGGGGATTCTCTGCTCTTTTCTATTGCATGTGCACAGGTTATGTACGCCTTTGTAATGCGTCCTGAGAGCTTGCCTAAATCTTATCATGAATTTATCCAGAAGACTGGTCCTGTTGCTCAGCCTGTATACAAGGCCGTAAGGGATTGTTGTAGAGGATACCCAGTGGATATTGCCTCACTGGCTGATTACTTGGCTGAGAAAGGGAGAAGCGATATAAAGTTGGAAGAGTTTCCATCCATTTTGCCTTGTTCTGTTATTCATCCCCACACAAATTCTTGTTTAGTGCACAATGCAACTGCAACATCAGCTACATTTAGGAAAACATTTCCGCTTTACTTCTCTTTGACGTTTGTACCATTTGTCGTTCTCCGCCTACAGAAGTTCCTGGCGGCCCCTGCTCGCACCTTTATGTTTGCTGTTAAAGATTCTGTTCGCTCAACAGCATTCTTGTCTGCTTTCGTTGGAGTCTTTCAGGGTGTCATATGTTTGCACAGAAAAGTTGCATCAAAAGACAACAAGCTTGTATATTGGATTGCTGGTGGTCTATCTGCTCTTTCAGTTCTACTGGAGAAAAAGGCTAGGCGTGGTGAACTTGCTCTATATGTTCTTCCACGGGCAGGAGATTCTTTGTGGTATATTTTAGTAAACCGTCACCTGCTTCCAGATATTAAGAATGCTGAGGTGTTCTTGTTCTGTTTGTGTATGGGAGGAATTATGTACTACTTGGAACATGAGCCAGACACTATGGCTCCATTCCTGAGGAGTCTGATTCGCCGCTTTCTTGCTAGCCGAATTAGTAATCCAGTCAATTCGTCTAATAGGAATGCTTCCTACACATATTTGCAAACTCTTGATGCCATGAAGAAGCCAAAGCTATTAGAGAATGATGGTTCTGAAGCACCTTCTGAGAAGAAATACAATCTTGAATCAATACCAGGGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3143

Protein Analysis

526

Amino Acids

58.74

Weight (kDa)

9.44

Isoelectric Point (pI)

55.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 76 - 168 9.5e-10 Tim17/Tim22/Tim23/Pmp24 family
TMEM135_C_rich PF15982 297 - 425 1.5e-08 N-terminal cysteine-rich region of Transmembrane protein 135
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 582
AarI CACCTGC 1 cut(s) 1299
Acc36I ACCTGC 2 cut(s) 473, 1299
AccI GTMKAC 1 cut(s) 762
AccII CGCG 4 cut(s) 84, 105, 282, 284
AcsI RAATTY 2 cut(s) 725, 907
AcuI CTGAAG 1 cut(s) 1551
AcyI GRCGYC 3 cut(s) 60, 108, 252
AfaI GTAC 4 cut(s) 446, 680, 990, 1355
AfiI CCNNNNNNNGG 2 cut(s) 85, 1253
AflII CTTAAG 1 cut(s) 329
AgsI TTSAA 3 cut(s) 469, 489, 1562
AjnI CCWGG 2 cut(s) 1020, 1570
AluBI AGCT 6 cut(s) 234, 292, 705, 944, 1156, 1510
AluI AGCT 6 cut(s) 234, 292, 705, 944, 1156, 1510
Alw21I GWGCWC 2 cut(s) 670, 924
Alw26I GTCTC 3 cut(s) 21, 346, 452
Alw44I GTGCAC 2 cut(s) 666, 920
AoxI GGCC 3 cut(s) 87, 768, 1026
ApaLI GTGCAC 2 cut(s) 666, 920
ApeKI GCWGC 1 cut(s) 146
ApoI RAATTY 2 cut(s) 725, 907
Asp700I GAANNNNTTC 1 cut(s) 864
AspLEI GCGC 4 cut(s) 129, 138, 284, 537
AspS9I GGNCC 3 cut(s) 94, 743, 1027
AsuHPI GGTGA 2 cut(s) 1235, 1280
AsuNHI GCTAGC 1 cut(s) 1421
AvaII GGWCC 2 cut(s) 94, 743
AxyI CCTNAGG 1 cut(s) 1394
BaeGI GKGCMC 2 cut(s) 670, 924
BaeI ACNNNNGTAYC 2 cut(s) 972, 1005
BbsI GAAGAC 1 cut(s) 743
Bbv12I GWGCWC 2 cut(s) 670, 924
BbvCI CCTCAGC 1 cut(s) 1314
BbvI GCAGC 1 cut(s) 158
BccI CCATC 2 cut(s) 877, 1517
BceAI ACGGC 1 cut(s) 755
BciT130I CCWGG 2 cut(s) 1022, 1572
BciVI GTATCC 1 cut(s) 785
BcoDI GTCTC 3 cut(s) 21, 346, 452
BfaI CTAG 3 cut(s) 56, 1215, 1422
BfmI CTRYAG 1 cut(s) 1010
BfoI RGCGCY 1 cut(s) 130
BfrI CTTAAG 1 cut(s) 329
BfuAI ACCTGC 2 cut(s) 473, 1299
BfuI GTATCC 1 cut(s) 785
BglI GCCNNNNNGGC 2 cut(s) 384, 815
BglII AGATCT 1 cut(s) 352
BlpI GCTNAGC 1 cut(s) 753
Bme1390I CCNGG 2 cut(s) 1022, 1572
Bme18I GGWCC 2 cut(s) 94, 743
BmgT120I GGNCC 3 cut(s) 94, 743, 1027
BmiI GGNNCC 4 cut(s) 157, 221, 1029, 1386
BmrFI CCNGG 2 cut(s) 1022, 1572
BmrI ACTGGG 1 cut(s) 791
BmsI GCATC 3 cut(s) 419, 1148, 1480
BmtI GCTAGC 1 cut(s) 1425
BmuI ACTGGG 1 cut(s) 791
BpiI GAAGAC 1 cut(s) 743
BpmI CTGGAG 3 cut(s) 107, 649, 1223
Bpu10I CCTNAGC 1 cut(s) 1314
Bpu1102I GCTNAGC 1 cut(s) 753
BsaHI GRCGYC 3 cut(s) 60, 108, 252
BsaJI CCNNGG 4 cut(s) 90, 151, 1247, 1571
BsaXI ACNNNNNCTCC 2 cut(s) 1369, 1399
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 1253
Bse1I ACTGG 6 cut(s) 632, 745, 797, 819, 1206, 1439
Bse21I CCTNAGG 1 cut(s) 1394
BseBI CCWGG 2 cut(s) 1022, 1572
BseDI CCNNGG 4 cut(s) 90, 151, 1247, 1571
BseGI GGATG 2 cut(s) 869, 895
BseLI CCNNNNNNNGG 2 cut(s) 85, 1253
BseMII CTCAG 6 cut(s) 690, 767, 822, 1305, 1385, 1533
BseNI ACTGG 6 cut(s) 632, 745, 797, 819, 1206, 1439
BseRI GAGGAG 2 cut(s) 230, 1411
BseSI GKGCMC 2 cut(s) 670, 924
BseXI GCAGC 1 cut(s) 158
BseYI CCCAGC 1 cut(s) 159
Bsh1236I CGCG 4 cut(s) 84, 105, 282, 284
BshFI GGCC 3 cut(s) 89, 770, 1028
BsiHKAI GWGCWC 2 cut(s) 670, 924
BslI CCNNNNNNNGG 2 cut(s) 85, 1253
BsmAI GTCTC 3 cut(s) 21, 346, 452
BsmBI CGTCTC 1 cut(s) 21
BsmI GAATGC 4 cut(s) 475, 1079, 1315, 1465
BsnI GGCC 3 cut(s) 89, 770, 1028
Bsp1286I GDGCHC 2 cut(s) 670, 924
Bsp143I GATC 2 cut(s) 325, 352
Bsp1720I GCTNAGC 1 cut(s) 753
BspANI GGCC 3 cut(s) 89, 770, 1028
BspCNI CTCAG 6 cut(s) 691, 766, 823, 1306, 1386, 1534
BspFNI CGCG 4 cut(s) 84, 105, 282, 284
BspHI TCATGA 1 cut(s) 721
BspLI GGNNCC 4 cut(s) 157, 221, 1029, 1386
BspMI ACCTGC 2 cut(s) 473, 1299
BspOI GCTAGC 1 cut(s) 1425
BspTI CTTAAG 1 cut(s) 329
BsrI ACTGG 6 cut(s) 632, 745, 797, 819, 1206, 1439
BssECI CCNNGG 4 cut(s) 90, 151, 1247, 1571
BssMI GATC 2 cut(s) 325, 352
BssNAI GTATAC 1 cut(s) 763
BssNI GRCGYC 3 cut(s) 60, 108, 252
Bst1107I GTATAC 1 cut(s) 763
Bst2UI CCWGG 2 cut(s) 1022, 1572
Bst4CI ACNGT 2 cut(s) 573, 1286
Bst6I CTCTTC 2 cut(s) 319, 855
BstACI GRCGYC 3 cut(s) 60, 108, 252
BstAFI CTTAAG 1 cut(s) 329
BstAPI GCANNNNNTGC 1 cut(s) 575
BstC8I GCNNGC 7 cut(s) 134, 294, 298, 376, 707, 1036, 1423
BstDEI CTNAG 8 cut(s) 478, 597, 699, 753, 831, 1314, 1394, 1542
BstDSI CCRYGG 2 cut(s) 151, 1247
BstENI CCTNNNNNAGG 1 cut(s) 83
BstF5I GGATG 2 cut(s) 869, 895
BstFNI CGCG 4 cut(s) 84, 105, 282, 284
BstH2I RGCGCY 1 cut(s) 130
BstHHI GCGC 4 cut(s) 129, 138, 284, 537
BstKTI GATC 2 cut(s) 328, 355
BstMAI GTCTC 3 cut(s) 21, 346, 452
BstMBI GATC 2 cut(s) 325, 352
BstNI CCWGG 2 cut(s) 1022, 1572
BstNSI RCATGY 2 cut(s) 378, 666
BstSCI CCNGG 2 cut(s) 1020, 1570
BstSFI CTRYAG 1 cut(s) 1010
BstSLI GKGCMC 2 cut(s) 670, 924
BstUI CGCG 4 cut(s) 84, 105, 282, 284
BstV1I GCAGC 1 cut(s) 158
BstV2I GAAGAC 1 cut(s) 743
BstX2I RGATCY 1 cut(s) 352
BstXI CCANNNNNNTGG 2 cut(s) 215, 740
BstYI RGATCY 1 cut(s) 352
BstZ17I GTATAC 1 cut(s) 763
Bsu36I CCTNAGG 1 cut(s) 1394
BsuI GTATCC 1 cut(s) 785
BsuRI GGCC 3 cut(s) 89, 770, 1028
BtgI CCRYGG 2 cut(s) 151, 1247
BtsCI GGATG 2 cut(s) 869, 895
BtsIMutI CAGTG 3 cut(s) 625, 804, 812
BveI ACCTGC 2 cut(s) 473, 1299
Cac8I GCNNGC 7 cut(s) 134, 294, 298, 376, 707, 1036, 1423
CciI TCATGA 1 cut(s) 721
CfoI GCGC 4 cut(s) 129, 138, 284, 537
Cfr13I GGNCC 3 cut(s) 94, 743, 1027
CseI GACGC 5 cut(s) 68, 116, 190, 241, 683
Csp6I GTAC 4 cut(s) 445, 679, 989, 1354
CviAII CATG 7 cut(s) 375, 419, 635, 663, 722, 1367, 1495
CviQI GTAC 4 cut(s) 445, 679, 989, 1354
DdeI CTNAG 8 cut(s) 478, 597, 699, 753, 831, 1314, 1394, 1542
DpnI GATC 2 cut(s) 327, 354
DpnII GATC 2 cut(s) 325, 352
Eam1104I CTCTTC 2 cut(s) 319, 855
EarI CTCTTC 2 cut(s) 319, 855
EciI GGCGGA 1 cut(s) 995
Eco47I GGWCC 2 cut(s) 94, 743
Eco57I CTGAAG 1 cut(s) 1551
Eco81I CCTNAGG 1 cut(s) 1394
EcoNI CCTNNNNNAGG 1 cut(s) 83
EcoRII CCWGG 2 cut(s) 1020, 1570
Esp3I CGTCTC 1 cut(s) 21
FaeI CATG 7 cut(s) 378, 422, 638, 666, 725, 1370, 1498
FalI AAGNNNNNCTT 2 cut(s) 596, 628
FatI CATG 7 cut(s) 374, 418, 634, 662, 721, 1366, 1494
FauI CCCGC 2 cut(s) 48, 487
FauNDI CATATG 1 cut(s) 1118
FblI GTMKAC 1 cut(s) 762
FokI GGATG 2 cut(s) 856, 882
FspBI CTAG 3 cut(s) 56, 1215, 1422
GlaI GCGC 4 cut(s) 128, 137, 283, 536
GsaI CCCAGC 1 cut(s) 163
GsuI CTGGAG 3 cut(s) 107, 649, 1223
HaeII RGCGCY 1 cut(s) 130
HaeIII GGCC 3 cut(s) 89, 770, 1028
HgaI GACGC 5 cut(s) 68, 116, 190, 241, 683
HhaI GCGC 4 cut(s) 129, 138, 284, 537
Hin1I GRCGYC 3 cut(s) 60, 108, 252
Hin1II CATG 7 cut(s) 378, 422, 638, 666, 725, 1370, 1498
Hin6I GCGC 4 cut(s) 127, 136, 282, 535
HinP1I GCGC 4 cut(s) 127, 136, 282, 535
HindIII AAGCTT 1 cut(s) 1154
HphI GGTGA 2 cut(s) 1235, 1280
Hpy166II GTNNAC 5 cut(s) 668, 763, 922, 1226, 1282
Hpy188I TCNGA 7 cut(s) 73, 257, 346, 357, 1404, 1531, 1543
Hpy188III TCNNGA 7 cut(s) 698, 722, 733, 1298, 1393, 1487, 1559
Hpy8I GTNNAC 5 cut(s) 668, 763, 922, 1226, 1282
Hpy99I CGWCG 5 cut(s) 10, 16, 110, 194, 206
HpyAV CCTTC 2 cut(s) 525, 1548
HpyCH4III ACNGT 2 cut(s) 573, 1286
HpyCH4IV ACGT 1 cut(s) 983
HpyF3I CTNAG 8 cut(s) 478, 597, 699, 753, 831, 1314, 1394, 1542
HpySE526I ACGT 1 cut(s) 983
Hsp92I GRCGYC 3 cut(s) 60, 108, 252
Hsp92II CATG 7 cut(s) 378, 422, 638, 666, 725, 1370, 1498
HspAI GCGC 4 cut(s) 127, 136, 282, 535
Kzo9I GATC 2 cut(s) 325, 352
LmnI GCTCC 2 cut(s) 161, 1390
Lsp1109I GCAGC 1 cut(s) 158
LweI GCATC 3 cut(s) 419, 1148, 1480
MaeI CTAG 3 cut(s) 56, 1215, 1422
MaeII ACGT 1 cut(s) 983
MaeIII GTNAC 1 cut(s) 1286
MalI GATC 2 cut(s) 327, 354
MboI GATC 2 cut(s) 325, 352
MboII GAAGA 9 cut(s) 266, 269, 306, 380, 748, 872, 1235, 1510, 1558
MfeI CAATTG 1 cut(s) 489
MflI RGATCY 1 cut(s) 352
MhlI GDGCHC 2 cut(s) 670, 924
MluCI AATT 8 cut(s) 381, 489, 583, 725, 907, 1347, 1428, 1444
MlyI GAGTC 4 cut(s) 203, 515, 1110, 1408
MmeI TCCRAC 3 cut(s) 96, 837, 1078
MroXI GAANNNNTTC 1 cut(s) 864
MseI TTAA 4 cut(s) 288, 330, 1056, 1305
MslI CAYNNNNRTG 1 cut(s) 1043
MspCI CTTAAG 1 cut(s) 329
MspR9I CCNGG 2 cut(s) 1022, 1572
MunI CAATTG 1 cut(s) 489
Mva1269I GAATGC 4 cut(s) 475, 1079, 1315, 1465
MvaI CCWGG 2 cut(s) 1022, 1572
MvnI CGCG 4 cut(s) 84, 105, 282, 284
NdeI CATATG 1 cut(s) 1118
NdeII GATC 2 cut(s) 325, 352
NheI GCTAGC 1 cut(s) 1421
NlaIII CATG 7 cut(s) 378, 422, 638, 666, 725, 1370, 1498
NlaIV GGNNCC 4 cut(s) 157, 221, 1029, 1386
NmeAIII GCCGAG 1 cut(s) 115
NmuCI GTSAC 1 cut(s) 1286
NspI RCATGY 2 cut(s) 378, 666
PaeI GCATGC 1 cut(s) 378
PagI TCATGA 1 cut(s) 721
PaqCI CACCTGC 1 cut(s) 1299
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 198
PctI GAATGC 4 cut(s) 475, 1079, 1315, 1465
PdmI GAANNNNTTC 1 cut(s) 864
PfeI GAWTC 6 cut(s) 271, 641, 1061, 1259, 1405, 1562
PleI GAGTC 4 cut(s) 202, 515, 1109, 1407
PpsI GAGTC 4 cut(s) 202, 515, 1109, 1407
PsiI TTATAA 1 cut(s) 582
Psp6I CCWGG 2 cut(s) 1020, 1570
PspFI CCCAGC 1 cut(s) 159
PspGI CCWGG 2 cut(s) 1020, 1570
PspN4I GGNNCC 4 cut(s) 157, 221, 1029, 1386
PspPI GGNCC 3 cut(s) 94, 743, 1027
PsuI RGATCY 1 cut(s) 352
RsaI GTAC 4 cut(s) 446, 680, 990, 1355
RsaNI GTAC 4 cut(s) 445, 679, 989, 1354
RseI CAYNNNNRTG 1 cut(s) 1043
SaqAI TTAA 4 cut(s) 288, 330, 1056, 1305
Sau3AI GATC 2 cut(s) 325, 352
Sau96I GGNCC 3 cut(s) 94, 743, 1027
SchI GAGTC 4 cut(s) 203, 515, 1110, 1408
ScrFI CCNGG 2 cut(s) 1022, 1572
SduI GDGCHC 2 cut(s) 670, 924
SfaNI GCATC 3 cut(s) 419, 1148, 1480
SfcI CTRYAG 1 cut(s) 1010
SinI GGWCC 2 cut(s) 94, 743
SmiMI CAYNNNNRTG 1 cut(s) 1043
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
SphI GCATGC 1 cut(s) 378
Sse9I AATT 8 cut(s) 381, 489, 583, 725, 907, 1347, 1428, 1444
SspMI CTAG 3 cut(s) 56, 1215, 1422
StyD4I CCNGG 2 cut(s) 1020, 1570
TaaI ACNGT 2 cut(s) 573, 1286
TaiI ACGT 1 cut(s) 986
TaqI TCGA 1 cut(s) 324
TasI AATT 8 cut(s) 381, 489, 583, 725, 907, 1347, 1428, 1444
TatI WGTACW 2 cut(s) 444, 1353
TauI GCSGC 9 cut(s) 46, 49, 52, 65, 68, 132, 146, 1028, 1413
TfiI GAWTC 6 cut(s) 271, 641, 1061, 1259, 1405, 1562
Tru1I TTAA 4 cut(s) 288, 330, 1056, 1305
Tru9I TTAA 4 cut(s) 288, 330, 1056, 1305
TscAI CASTG 3 cut(s) 632, 804, 819
TseFI GTSAC 1 cut(s) 1286
TseI GCWGC 1 cut(s) 146
Tsp45I GTSAC 1 cut(s) 1286
TspDTI ATGAA 3 cut(s) 738, 884, 1511
TspRI CASTG 3 cut(s) 632, 804, 819
Vha464I CTTAAG 1 cut(s) 329
VneI GTGCAC 2 cut(s) 666, 920
VpaK11BI GGWCC 2 cut(s) 94, 743
XagI CCTNNNNNAGG 1 cut(s) 83
XapI RAATTY 2 cut(s) 725, 907
XceI RCATGY 2 cut(s) 378, 666
XcmI CCANNNNNNNNNTGG 1 cut(s) 632
XmiI GTMKAC 1 cut(s) 762
XmnI GAANNNNTTC 1 cut(s) 864
XspI CTAG 3 cut(s) 56, 1215, 1422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.