MD07G1283300.v1.1

Protein of unknown function (DUF3143)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010374
Physical Location & Seq
Forward (+)
34623657 .. 34625492
1836 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1283300.v1.1.491

Sequence Viewer

Length: 510 bp
ATGTCCGCCATCACCTTCTCCTCCATTTCTGTCTCCAAATTCATCACCTTCTCCTCCATTTCTTCCTCAAGTTTCCCAAAGTATCCAACTCCGTACCCTTCACTTTCACCCAAATTCCCACCGTATCCCCTCACTCACTCTTCCAGAAAGCTCTCGGTCCCGGTGTTTTCCAAGCCCTCCGAGGCGGAGGAAGAAGAGGAGCAGTCGTCGGCGCCGGAGGACGAGTGGCTGAAGAAGCTGCCGGACAAGAAGAAGCCGCTGTACGCCCACAGCCTCCCCTGCATCGAGGCCTGGCTGAGGAACTTGGGGTTTTACCAGAGCGAAGAGGACCGGGCGGTTTGGCTGGTGGAGAAGCCCGAGTGGCACGCGCAGCTCTCCCTCGACGTCACCGATTTGTATGTGAGGTATCTAAAGACTGGACCGGGAAATATTGAAAAGGATGTGGAGAGGAGATTTAGTTATGCACTGAGCAGAGAGGACATTGAGAATGCAGTGCTTGGAGGGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3143

Protein Analysis

170

Amino Acids

19.23

Weight (kDa)

5.47

Isoelectric Point (pI)

79.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3143 PF11341 102 - 168 8.5e-24 Protein of unknown function (DUF3143)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 387
AccB1I GGYRCC 1 cut(s) 211
AccII CGCG 1 cut(s) 368
AciI CCGC 4 cut(s) 6, 185, 257, 335
AcsI RAATTY 2 cut(s) 38, 113
AcuI CTGAAG 1 cut(s) 251
AcyI GRCGYC 2 cut(s) 212, 384
AfaI GTAC 2 cut(s) 95, 263
AfiI CCNNNNNNNGG 1 cut(s) 297
AgsI TTSAA 1 cut(s) 434
AjnI CCWGG 1 cut(s) 290
AluBI AGCT 3 cut(s) 151, 238, 373
AluI AGCT 3 cut(s) 151, 238, 373
Alw26I GTCTC 1 cut(s) 37
Ama87I CYCGRG 1 cut(s) 356
AoxI GGCC 2 cut(s) 288, 503
ApeKI GCWGC 2 cut(s) 238, 370
ApoI RAATTY 2 cut(s) 38, 113
AspLEI GCGC 2 cut(s) 214, 370
AspS9I GGNCC 4 cut(s) 157, 328, 419, 503
AsuC2I CCSGG 3 cut(s) 161, 332, 423
AsuHPI GGTGA 4 cut(s) 4, 37, 99, 379
AvaI CYCGRG 1 cut(s) 356
AvaII GGWCC 3 cut(s) 157, 328, 419
BanI GGYRCC 1 cut(s) 211
BarI GAAGNNNNNNTAC 2 cut(s) 245, 277
BbvCI CCTCAGC 1 cut(s) 296
BbvI GCAGC 2 cut(s) 225, 382
BccI CCATC 1 cut(s) 17
BciT130I CCWGG 1 cut(s) 292
BciVI GTATCC 2 cut(s) 93, 135
BcnI CCSGG 3 cut(s) 161, 332, 423
BcoDI GTCTC 1 cut(s) 37
BfoI RGCGCY 1 cut(s) 215
BfuI GTATCC 2 cut(s) 93, 135
BglI GCCNNNNNGGC 1 cut(s) 361
BisI GCNGC 3 cut(s) 239, 257, 371
BlsI GCNGC 3 cut(s) 240, 258, 372
Bme1390I CCNGG 4 cut(s) 161, 292, 332, 423
Bme18I GGWCC 3 cut(s) 157, 328, 419
BmeT110I CYCGRG 1 cut(s) 356
BmgT120I GGNCC 4 cut(s) 157, 328, 419, 503
BmiI GGNNCC 2 cut(s) 159, 213
BmrFI CCNGG 4 cut(s) 161, 292, 332, 423
BmsI GCATC 1 cut(s) 291
Bpu10I CCTNAGC 1 cut(s) 296
BpuEI CTTGAG 1 cut(s) 52
BpuMI CCSGG 3 cut(s) 161, 332, 423
BsaHI GRCGYC 2 cut(s) 212, 384
BsaJI CCNNGG 1 cut(s) 180
BsaXI ACNNNNNCTCC 6 cut(s) 191, 209, 221, 239, 442, 472
Bsc4I CCNNNNNNNGG 1 cut(s) 297
Bse1I ACTGG 1 cut(s) 421
BseBI CCWGG 1 cut(s) 292
BseDI CCNNGG 1 cut(s) 180
BseGI GGATG 1 cut(s) 445
BseLI CCNNNNNNNGG 1 cut(s) 297
BseMII CTCAG 2 cut(s) 287, 458
BseNI ACTGG 1 cut(s) 421
BseRI GAGGAG 4 cut(s) 10, 43, 212, 463
BseXI GCAGC 2 cut(s) 225, 382
Bsh1236I CGCG 1 cut(s) 368
BshFI GGCC 2 cut(s) 290, 505
BshNI GGYRCC 1 cut(s) 211
BsiHKCI CYCGRG 1 cut(s) 356
BsiSI CCGG 5 cut(s) 161, 215, 242, 331, 422
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 1 cut(s) 297
BsmAI GTCTC 1 cut(s) 37
BsmFI GGGAC 1 cut(s) 143
BsmI GAATGC 1 cut(s) 493
BsnI GGCC 2 cut(s) 290, 505
BsoBI CYCGRG 1 cut(s) 356
BspACI CCGC 4 cut(s) 6, 185, 257, 335
BspANI GGCC 2 cut(s) 290, 505
BspCNI CTCAG 2 cut(s) 288, 459
BspFNI CGCG 1 cut(s) 368
BspLI GGNNCC 2 cut(s) 159, 213
BspT107I GGYRCC 1 cut(s) 211
BsrI ACTGG 1 cut(s) 421
BssECI CCNNGG 1 cut(s) 180
BssNI GRCGYC 2 cut(s) 212, 384
Bst2UI CCWGG 1 cut(s) 292
Bst4CI ACNGT 1 cut(s) 123
Bst6I CTCTTC 3 cut(s) 145, 189, 318
BstACI GRCGYC 2 cut(s) 212, 384
BstC8I GCNNGC 1 cut(s) 366
BstDEI CTNAG 2 cut(s) 296, 467
BstENI CCTNNNNNAGG 1 cut(s) 295
BstF5I GGATG 1 cut(s) 445
BstFNI CGCG 1 cut(s) 368
BstH2I RGCGCY 1 cut(s) 215
BstHHI GCGC 2 cut(s) 214, 370
BstMAI GTCTC 1 cut(s) 37
BstMWI GCNNNNNNNGC 5 cut(s) 235, 279, 361, 370, 502
BstNI CCWGG 1 cut(s) 292
BstSCI CCNGG 4 cut(s) 159, 290, 330, 421
BstUI CGCG 1 cut(s) 368
BstV1I GCAGC 2 cut(s) 225, 382
BsuI GTATCC 2 cut(s) 93, 135
BsuRI GGCC 2 cut(s) 290, 505
BtsCI GGATG 1 cut(s) 445
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 2 cut(s) 464, 498
Cac8I GCNNGC 1 cut(s) 366
CfoI GCGC 2 cut(s) 214, 370
Cfr13I GGNCC 4 cut(s) 157, 328, 419, 503
Csp6I GTAC 2 cut(s) 94, 262
CviAII CATG 1 cut(s) 507
CviQI GTAC 2 cut(s) 94, 262
DdeI CTNAG 2 cut(s) 296, 467
DinI GGCGCC 1 cut(s) 213
Eam1104I CTCTTC 3 cut(s) 145, 189, 318
EarI CTCTTC 3 cut(s) 145, 189, 318
EciI GGCGGA 1 cut(s) 200
Eco147I AGGCCT 1 cut(s) 290
Eco47I GGWCC 3 cut(s) 157, 328, 419
Eco57I CTGAAG 1 cut(s) 251
Eco88I CYCGRG 1 cut(s) 356
EcoNI CCTNNNNNAGG 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 290
EgeI GGCGCC 1 cut(s) 213
EheI GGCGCC 1 cut(s) 213
FaeI CATG 1 cut(s) 510
FaiI YATR 3 cut(s) 399, 462, 508
FaqI GGGAC 1 cut(s) 143
FatI CATG 1 cut(s) 506
Fnu4HI GCNGC 3 cut(s) 239, 257, 371
FokI GGATG 1 cut(s) 452
Fsp4HI GCNGC 3 cut(s) 239, 257, 371
GlaI GCGC 2 cut(s) 213, 369
GluI GCNGC 3 cut(s) 239, 257, 371
HaeII RGCGCY 1 cut(s) 215
HaeIII GGCC 2 cut(s) 290, 505
HapII CCGG 5 cut(s) 161, 215, 242, 331, 422
HhaI GCGC 2 cut(s) 214, 370
Hin1I GRCGYC 2 cut(s) 212, 384
Hin1II CATG 1 cut(s) 510
Hin6I GCGC 2 cut(s) 212, 368
HinP1I GCGC 2 cut(s) 212, 368
HpaII CCGG 5 cut(s) 161, 215, 242, 331, 422
HphI GGTGA 4 cut(s) 4, 37, 99, 379
Hpy188I TCNGA 1 cut(s) 181
Hpy188III TCNNGA 1 cut(s) 144
Hpy99I CGWCG 2 cut(s) 211, 386
HpyAV CCTTC 3 cut(s) 25, 58, 108
HpyCH4III ACNGT 1 cut(s) 123
HpyCH4IV ACGT 1 cut(s) 384
HpyCH4V TGCA 3 cut(s) 282, 464, 491
HpyF10VI GCNNNNNNNGC 5 cut(s) 235, 279, 361, 370, 502
HpyF3I CTNAG 2 cut(s) 296, 467
HpySE526I ACGT 1 cut(s) 384
Hsp92I GRCGYC 2 cut(s) 212, 384
Hsp92II CATG 1 cut(s) 510
HspAI GCGC 2 cut(s) 212, 368
KasI GGCGCC 1 cut(s) 211
LmnI GCTCC 1 cut(s) 199
Lsp1109I GCAGC 2 cut(s) 225, 382
LweI GCATC 1 cut(s) 291
MaeII ACGT 1 cut(s) 384
MaeIII GTNAC 1 cut(s) 385
MboII GAAGA 7 cut(s) 54, 132, 203, 206, 244, 262, 335
MluCI AATT 2 cut(s) 38, 113
Mly113I GGCGCC 1 cut(s) 212
MmeI TCCRAC 1 cut(s) 110
MspA1I CMGCKG 1 cut(s) 259
MspI CCGG 5 cut(s) 161, 215, 242, 331, 422
MspR9I CCNGG 4 cut(s) 161, 292, 332, 423
Mva1269I GAATGC 1 cut(s) 493
MvaI CCWGG 1 cut(s) 292
MvnI CGCG 1 cut(s) 368
MwoI GCNNNNNNNGC 5 cut(s) 235, 279, 361, 370, 502
NarI GGCGCC 1 cut(s) 212
NciI CCSGG 3 cut(s) 161, 332, 423
NlaIII CATG 1 cut(s) 510
NlaIV GGNNCC 2 cut(s) 159, 213
NmuCI GTSAC 1 cut(s) 385
PceI AGGCCT 1 cut(s) 290
PcsI WCGNNNNNNNCGW 1 cut(s) 387
PctI GAATGC 1 cut(s) 493
PkrI GCNGC 3 cut(s) 240, 258, 372
PluTI GGCGCC 1 cut(s) 215
Psp6I CCWGG 1 cut(s) 290
PspGI CCWGG 1 cut(s) 290
PspN4I GGNNCC 2 cut(s) 159, 213
PspPI GGNCC 4 cut(s) 157, 328, 419, 503
RsaI GTAC 2 cut(s) 95, 263
RsaNI GTAC 2 cut(s) 94, 262
SatI GCNGC 3 cut(s) 239, 257, 371
Sau96I GGNCC 4 cut(s) 157, 328, 419, 503
ScrFI CCNGG 4 cut(s) 161, 292, 332, 423
SetI ASST 7 cut(s) 17, 50, 153, 240, 375, 387, 407
SfaNI GCATC 1 cut(s) 291
SfoI GGCGCC 1 cut(s) 213
SinI GGWCC 3 cut(s) 157, 328, 419
SmlI CTYRAG 1 cut(s) 67
SmoI CTYRAG 1 cut(s) 67
Sse9I AATT 2 cut(s) 38, 113
SseBI AGGCCT 1 cut(s) 290
SsiI CCGC 4 cut(s) 6, 185, 257, 335
SspDI GGCGCC 1 cut(s) 211
SspI AATATT 1 cut(s) 430
StuI AGGCCT 1 cut(s) 290
StyD4I CCNGG 4 cut(s) 159, 290, 330, 421
TaaI ACNGT 1 cut(s) 123
TaiI ACGT 1 cut(s) 387
TaqI TCGA 2 cut(s) 285, 381
TaqII GACCGA 1 cut(s) 145
TasI AATT 2 cut(s) 38, 113
TauI GCSGC 1 cut(s) 259
TscAI CASTG 2 cut(s) 471, 498
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 2 cut(s) 238, 370
Tsp45I GTSAC 1 cut(s) 385
TspDTI ATGAA 1 cut(s) 31
TspGWI ACGGA 1 cut(s) 81
TspRI CASTG 2 cut(s) 471, 498
VpaK11BI GGWCC 3 cut(s) 157, 328, 419
XagI CCTNNNNNAGG 1 cut(s) 295
XapI RAATTY 2 cut(s) 38, 113
ZraI GACGTC 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.