FvH4_7g30800

Protein of unknown function (DUF3143)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0010374
Physical Location & Seq
Forward (+)
22346123 .. 22348621
2499 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g30800.t1

Sequence Viewer

Length: 423 bp
ATGTCGACAGTCTTCTTCTCCGTTTCCAATTTGATCTTCCTCTCAAATCCAAACTACTCTGTTCGCTACTCACTCTCGCCTAAAGCGACGCCGTTTCGGCTCGTTCGGTCTTCTCGAAGGAGGCTAGCGATTCCGGTGTGTTCGAAGTCGTCGGAGGCGGAGGAGGTCTCGGCGGCGCCGGAGGACGAGTGGCTGAAGCGGCTGCCGGACAAGAAGAAGCCTCTGTATTCTCACAGTCTGCCTTGCATTGAGGCGTGGCTGAGAAGCTTGGGGTTCTTCCAGAGCAGAGAGGACCGCGCCGTTTGGCTGATCGACAAGCCGGAGTGGCACGCGCAGCTCTCACTCGACGTCACCGATCTCTATGTCAGACTGGACCTGGAAATCTTGAAAAGGATATGGAGAGGAGATTTAGCTATGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3143

Protein Analysis

141

Amino Acids

16.32

Weight (kDa)

9.23

Isoelectric Point (pI)

66.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3143 PF11341 90 - 123 5.4e-08 Protein of unknown function (DUF3143)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 351
AccB1I GGYRCC 1 cut(s) 175
AccI GTMKAC 1 cut(s) 5
AccII CGCG 2 cut(s) 297, 332
AciI CCGC 4 cut(s) 158, 173, 199, 295
AcuI CTGAAG 1 cut(s) 215
AcyI GRCGYC 3 cut(s) 89, 176, 348
AgsI TTSAA 1 cut(s) 388
AjnI CCWGG 1 cut(s) 375
AjuI GAANNNNNNNTTGG 2 cut(s) 20, 52
AluBI AGCT 3 cut(s) 267, 337, 413
AluI AGCT 3 cut(s) 267, 337, 413
Alw26I GTCTC 1 cut(s) 172
ApeKI GCWGC 2 cut(s) 202, 334
AspLEI GCGC 3 cut(s) 178, 299, 334
AspS9I GGNCC 2 cut(s) 292, 373
AsuHPI GGTGA 1 cut(s) 343
AsuII TTCGAA 1 cut(s) 143
AsuNHI GCTAGC 1 cut(s) 124
AvaII GGWCC 2 cut(s) 292, 373
BanI GGYRCC 1 cut(s) 175
BbsI GAAGAC 2 cut(s) 4, 102
BbvI GCAGC 2 cut(s) 189, 346
BceAI ACGGC 2 cut(s) 76, 284
BciT130I CCWGG 1 cut(s) 377
BcoDI GTCTC 1 cut(s) 172
BfaI CTAG 1 cut(s) 125
BfoI RGCGCY 1 cut(s) 179
BglI GCCNNNNNGGC 2 cut(s) 97, 325
BisI GCNGC 4 cut(s) 174, 200, 203, 335
BlsI GCNGC 4 cut(s) 175, 201, 204, 336
Bme1390I CCNGG 1 cut(s) 377
Bme18I GGWCC 2 cut(s) 292, 373
BmgT120I GGNCC 2 cut(s) 292, 373
BmiI GGNNCC 1 cut(s) 177
BmrFI CCNGG 1 cut(s) 377
BmtI GCTAGC 1 cut(s) 128
BpiI GAAGAC 2 cut(s) 4, 102
BplI GAGNNNNNCTC 2 cut(s) 152, 184
Bpu14I TTCGAA 1 cut(s) 143
BsaHI GRCGYC 3 cut(s) 89, 176, 348
BsaI GGTCTC 1 cut(s) 172
BsaWI WCCGGW 1 cut(s) 133
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bse1I ACTGG 1 cut(s) 375
BseBI CCWGG 1 cut(s) 377
BseMII CTCAG 1 cut(s) 251
BseNI ACTGG 1 cut(s) 375
BseRI GAGGAG 2 cut(s) 176, 417
BseXI GCAGC 2 cut(s) 189, 346
Bsh1236I CGCG 2 cut(s) 297, 332
BshNI GGYRCC 1 cut(s) 175
BsiSI CCGG 4 cut(s) 134, 179, 206, 320
BsmAI GTCTC 1 cut(s) 172
Bso31I GGTCTC 1 cut(s) 172
Bsp119I TTCGAA 1 cut(s) 143
Bsp143I GATC 3 cut(s) 33, 309, 355
BspACI CCGC 4 cut(s) 158, 173, 199, 295
BspCNI CTCAG 1 cut(s) 252
BspFNI CGCG 2 cut(s) 297, 332
BspLI GGNNCC 1 cut(s) 177
BspOI GCTAGC 1 cut(s) 128
BspT104I TTCGAA 1 cut(s) 143
BspT107I GGYRCC 1 cut(s) 175
BspTNI GGTCTC 1 cut(s) 172
BsrI ACTGG 1 cut(s) 375
BssMI GATC 3 cut(s) 33, 309, 355
BssNI GRCGYC 3 cut(s) 89, 176, 348
Bst2UI CCWGG 1 cut(s) 377
Bst4CI ACNGT 2 cut(s) 10, 236
BstACI GRCGYC 3 cut(s) 89, 176, 348
BstBI TTCGAA 1 cut(s) 143
BstC8I GCNNGC 2 cut(s) 126, 330
BstDEI CTNAG 1 cut(s) 260
BstFNI CGCG 2 cut(s) 297, 332
BstH2I RGCGCY 1 cut(s) 179
BstHHI GCGC 3 cut(s) 178, 299, 334
BstKTI GATC 3 cut(s) 36, 312, 358
BstMAI GTCTC 1 cut(s) 172
BstMBI GATC 3 cut(s) 33, 309, 355
BstMWI GCNNNNNNNGC 4 cut(s) 97, 199, 325, 334
BstNI CCWGG 1 cut(s) 377
BstSCI CCNGG 1 cut(s) 375
BstUI CGCG 2 cut(s) 297, 332
BstV1I GCAGC 2 cut(s) 189, 346
BstV2I GAAGAC 2 cut(s) 4, 102
BtsIMutI CAGTG 1 cut(s) 418
Cac8I GCNNGC 2 cut(s) 126, 330
CfoI GCGC 3 cut(s) 178, 299, 334
Cfr13I GGNCC 2 cut(s) 292, 373
CseI GACGC 1 cut(s) 97
DdeI CTNAG 1 cut(s) 260
DinI GGCGCC 1 cut(s) 177
DpnI GATC 3 cut(s) 35, 311, 357
DpnII GATC 3 cut(s) 33, 309, 355
EciI GGCGGA 1 cut(s) 173
Eco31I GGTCTC 1 cut(s) 172
Eco47I GGWCC 2 cut(s) 292, 373
Eco57I CTGAAG 1 cut(s) 215
EcoRII CCWGG 1 cut(s) 375
EgeI GGCGCC 1 cut(s) 177
EheI GGCGCC 1 cut(s) 177
FaiI YATR 3 cut(s) 363, 397, 416
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 174, 200, 203, 335
Fsp4HI GCNGC 4 cut(s) 174, 200, 203, 335
FspBI CTAG 1 cut(s) 125
GlaI GCGC 3 cut(s) 177, 298, 333
GluI GCNGC 4 cut(s) 174, 200, 203, 335
HaeII RGCGCY 1 cut(s) 179
HapII CCGG 4 cut(s) 134, 179, 206, 320
HgaI GACGC 1 cut(s) 97
HhaI GCGC 3 cut(s) 178, 299, 334
Hin1I GRCGYC 3 cut(s) 89, 176, 348
Hin6I GCGC 3 cut(s) 176, 297, 332
HinP1I GCGC 3 cut(s) 176, 297, 332
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 265
HinfI GANTC 1 cut(s) 130
HpaII CCGG 4 cut(s) 134, 179, 206, 320
HphI GGTGA 1 cut(s) 343
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 2 cut(s) 154, 368
Hpy188III TCNNGA 3 cut(s) 114, 280, 385
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 3 cut(s) 91, 154, 350
HpyAV CCTTC 1 cut(s) 111
HpyCH4III ACNGT 2 cut(s) 10, 236
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 2 cut(s) 246, 418
HpyF10VI GCNNNNNNNGC 4 cut(s) 97, 199, 325, 334
HpyF3I CTNAG 1 cut(s) 260
HpySE526I ACGT 1 cut(s) 348
Hsp92I GRCGYC 3 cut(s) 89, 176, 348
HspAI GCGC 3 cut(s) 176, 297, 332
KasI GGCGCC 1 cut(s) 175
Kzo9I GATC 3 cut(s) 33, 309, 355
LpnPI CCDG 8 cut(s) 147, 192, 219, 293, 333, 356, 362, 389
Lsp1109I GCAGC 2 cut(s) 189, 346
MaeI CTAG 1 cut(s) 125
MaeII ACGT 1 cut(s) 348
MaeIII GTNAC 1 cut(s) 349
MalI GATC 3 cut(s) 35, 311, 357
MboI GATC 3 cut(s) 33, 309, 355
MboII GAAGA 6 cut(s) 4, 7, 28, 102, 226, 268
MluCI AATT 1 cut(s) 28
Mly113I GGCGCC 1 cut(s) 176
MmeI TCCRAC 1 cut(s) 132
MspI CCGG 4 cut(s) 134, 179, 206, 320
MspR9I CCNGG 1 cut(s) 377
MvaI CCWGG 1 cut(s) 377
MvnI CGCG 2 cut(s) 297, 332
MwoI GCNNNNNNNGC 4 cut(s) 97, 199, 325, 334
NarI GGCGCC 1 cut(s) 176
NdeII GATC 3 cut(s) 33, 309, 355
NheI GCTAGC 1 cut(s) 124
NlaIV GGNNCC 1 cut(s) 177
NmeAIII GCCGAG 1 cut(s) 149
NmuCI GTSAC 1 cut(s) 349
NspV TTCGAA 1 cut(s) 143
PcsI WCGNNNNNNNCGW 3 cut(s) 83, 112, 351
PfeI GAWTC 1 cut(s) 130
PkrI GCNGC 4 cut(s) 175, 201, 204, 336
PluTI GGCGCC 1 cut(s) 179
Psp6I CCWGG 1 cut(s) 375
PspGI CCWGG 1 cut(s) 375
PspN4I GGNNCC 1 cut(s) 177
PspPI GGNCC 2 cut(s) 292, 373
SalI GTCGAC 1 cut(s) 4
SatI GCNGC 4 cut(s) 174, 200, 203, 335
Sau3AI GATC 3 cut(s) 33, 309, 355
Sau96I GGNCC 2 cut(s) 292, 373
ScrFI CCNGG 1 cut(s) 377
SetI ASST 6 cut(s) 168, 269, 339, 351, 378, 415
SfoI GGCGCC 1 cut(s) 177
SfuI TTCGAA 1 cut(s) 143
SinI GGWCC 2 cut(s) 292, 373
Sse9I AATT 1 cut(s) 28
SsiI CCGC 4 cut(s) 158, 173, 199, 295
SspDI GGCGCC 1 cut(s) 175
SspMI CTAG 1 cut(s) 125
StyD4I CCNGG 1 cut(s) 375
TaaI ACNGT 2 cut(s) 10, 236
TaiI ACGT 1 cut(s) 351
TaqI TCGA 5 cut(s) 5, 115, 143, 312, 345
TaqII GACCGA 1 cut(s) 96
TasI AATT 1 cut(s) 28
TauI GCSGC 2 cut(s) 176, 202
TfiI GAWTC 1 cut(s) 130
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 2 cut(s) 202, 334
Tsp45I GTSAC 1 cut(s) 349
TspGWI ACGGA 1 cut(s) 10
VpaK11BI GGWCC 2 cut(s) 292, 373
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 125
ZraI GACGTC 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.