MD01G1213500.v1.1

Protein of unknown function (DUF3143)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0010374
Physical Location & Seq
Forward (+)
30678837 .. 30680711
1875 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1213500.v1.1.491

Sequence Viewer

Length: 495 bp
ATGTCCGCCATTTCTCTCACCAAGTTCATCGTCTTCTCTTCCATCGCTTCCTCAACTTTCCCAAAATATCCAACTCCTTACCCTTCACTTTCACCCAAATGCCCAACGTATCCCCTCACCCACTCTTCCAGAAAGATTTCGGTCTCGGTATTTTCGAAGCCTTCCGAGGCGGAGGAAGACGAGGAGCTCCCGTCGGCGCCGGAGGACGAGTGGCTGAAGAAGCTGCCGGACAAGAAGAAGCCGCTGTACGCCCACAGCCTCCCCTGCATCGAGGCCTGGCTGAGGAACTTGGGGTTTTACCAGAGCAAAGAGGACCGGGCGGTTTGGCTGGTGGAGAAGCCCGAGTGGCACGCCCAGCTCTCGCTCGACATCACCGACTTGTATGTGAGGTATCTAAAGACTGGACCAGGAAACCTTGAAAAGGATATGGAGAGGCGATTTAGTTATGCGCTGAGCAGAGAGGACATCGAGAATGCAGTGCTTGGAGGACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3143

Protein Analysis

165

Amino Acids

18.7

Weight (kDa)

5.96

Isoelectric Point (pI)

59.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3143 PF11341 97 - 163 4.7e-24 Protein of unknown function (DUF3143)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 196
AciI CCGC 4 cut(s) 6, 170, 242, 320
AcuI CTGAAG 1 cut(s) 236
AcyI GRCGYC 1 cut(s) 197
AfaI GTAC 1 cut(s) 248
AfiI CCNNNNNNNGG 2 cut(s) 282, 421
AgsI TTSAA 1 cut(s) 419
AjnI CCWGG 2 cut(s) 275, 406
AluBI AGCT 3 cut(s) 187, 223, 358
AluI AGCT 3 cut(s) 187, 223, 358
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 1 cut(s) 148
Ama87I CYCGRG 1 cut(s) 341
AoxI GGCC 1 cut(s) 273
ApeKI GCWGC 1 cut(s) 223
Asp700I GAANNNNTTC 1 cut(s) 136
AspLEI GCGC 2 cut(s) 199, 451
AspS9I GGNCC 3 cut(s) 313, 404, 488
AsuC2I CCSGG 1 cut(s) 317
AsuHPI GGTGA 4 cut(s) 10, 84, 109, 364
AsuII TTCGAA 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 341
AvaII GGWCC 3 cut(s) 313, 404, 488
BanI GGYRCC 1 cut(s) 196
BanII GRGCYC 1 cut(s) 189
BarI GAAGNNNNNNTAC 2 cut(s) 230, 262
BbsI GAAGAC 2 cut(s) 25, 183
Bbv12I GWGCWC 1 cut(s) 189
BbvCI CCTCAGC 1 cut(s) 281
BbvI GCAGC 1 cut(s) 210
BccI CCATC 1 cut(s) 50
BciT130I CCWGG 2 cut(s) 277, 408
BciVI GTATCC 1 cut(s) 120
BcnI CCSGG 1 cut(s) 317
BcoDI GTCTC 1 cut(s) 148
BfoI RGCGCY 1 cut(s) 200
BfuI GTATCC 1 cut(s) 120
BglI GCCNNNNNGGC 1 cut(s) 346
BisI GCNGC 2 cut(s) 224, 242
BlpI GCTNAGC 1 cut(s) 452
BlsI GCNGC 2 cut(s) 225, 243
Bme1390I CCNGG 3 cut(s) 277, 317, 408
Bme18I GGWCC 3 cut(s) 313, 404, 488
BmeT110I CYCGRG 1 cut(s) 341
BmgT120I GGNCC 3 cut(s) 313, 404, 488
BmiI GGNNCC 2 cut(s) 198, 490
BmrFI CCNGG 3 cut(s) 277, 317, 408
BmsI GCATC 1 cut(s) 276
BpiI GAAGAC 2 cut(s) 25, 183
Bpu10I CCTNAGC 1 cut(s) 281
Bpu1102I GCTNAGC 1 cut(s) 452
Bpu14I TTCGAA 1 cut(s) 155
BpuMI CCSGG 1 cut(s) 317
BsaHI GRCGYC 1 cut(s) 197
BsaI GGTCTC 1 cut(s) 148
BsaJI CCNNGG 1 cut(s) 165
BsaXI ACNNNNNCTCC 4 cut(s) 176, 194, 206, 224
Bsc4I CCNNNNNNNGG 2 cut(s) 282, 421
Bse1I ACTGG 1 cut(s) 406
BseBI CCWGG 2 cut(s) 277, 408
BseDI CCNNGG 1 cut(s) 165
BseLI CCNNNNNNNGG 2 cut(s) 282, 421
BseMII CTCAG 2 cut(s) 272, 443
BseNI ACTGG 1 cut(s) 406
BseRI GAGGAG 1 cut(s) 197
BseXI GCAGC 1 cut(s) 210
BseYI CCCAGC 1 cut(s) 354
BshFI GGCC 1 cut(s) 275
BshNI GGYRCC 1 cut(s) 196
BsiHKAI GWGCWC 1 cut(s) 189
BsiHKCI CYCGRG 1 cut(s) 341
BsiSI CCGG 3 cut(s) 200, 227, 316
BslI CCNNNNNNNGG 2 cut(s) 282, 421
BsmAI GTCTC 1 cut(s) 148
BsmI GAATGC 1 cut(s) 478
BsnI GGCC 1 cut(s) 275
Bso31I GGTCTC 1 cut(s) 148
BsoBI CYCGRG 1 cut(s) 341
Bsp119I TTCGAA 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 189
Bsp1720I GCTNAGC 1 cut(s) 452
BspACI CCGC 4 cut(s) 6, 170, 242, 320
BspANI GGCC 1 cut(s) 275
BspCNI CTCAG 2 cut(s) 273, 444
BspLI GGNNCC 2 cut(s) 198, 490
BspT104I TTCGAA 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 196
BspTNI GGTCTC 1 cut(s) 148
BsrI ACTGG 1 cut(s) 406
BssECI CCNNGG 1 cut(s) 165
BssNI GRCGYC 1 cut(s) 197
Bst2UI CCWGG 2 cut(s) 277, 408
Bst6I CTCTTC 2 cut(s) 43, 130
BstACI GRCGYC 1 cut(s) 197
BstBI TTCGAA 1 cut(s) 155
BstC8I GCNNGC 1 cut(s) 351
BstDEI CTNAG 2 cut(s) 281, 452
BstENI CCTNNNNNAGG 2 cut(s) 280, 419
BstH2I RGCGCY 1 cut(s) 200
BstHHI GCGC 2 cut(s) 199, 451
BstMAI GTCTC 1 cut(s) 148
BstMWI GCNNNNNNNGC 4 cut(s) 220, 264, 346, 355
BstNI CCWGG 2 cut(s) 277, 408
BstSCI CCNGG 3 cut(s) 275, 315, 406
BstV1I GCAGC 1 cut(s) 210
BstV2I GAAGAC 2 cut(s) 25, 183
BsuI GTATCC 1 cut(s) 120
BsuRI GGCC 1 cut(s) 275
BtgZI GCGATG 1 cut(s) 28
BtsI GCAGTG 1 cut(s) 483
BtsIMutI CAGTG 1 cut(s) 483
Cac8I GCNNGC 1 cut(s) 351
CfoI GCGC 2 cut(s) 199, 451
Cfr13I GGNCC 3 cut(s) 313, 404, 488
Csp6I GTAC 1 cut(s) 247
CviQI GTAC 1 cut(s) 247
DdeI CTNAG 2 cut(s) 281, 452
DinI GGCGCC 1 cut(s) 198
Eam1104I CTCTTC 2 cut(s) 43, 130
EarI CTCTTC 2 cut(s) 43, 130
EciI GGCGGA 1 cut(s) 185
Ecl136II GAGCTC 1 cut(s) 187
Eco147I AGGCCT 1 cut(s) 275
Eco24I GRGCYC 1 cut(s) 189
Eco31I GGTCTC 1 cut(s) 148
Eco47I GGWCC 3 cut(s) 313, 404, 488
Eco53kI GAGCTC 1 cut(s) 187
Eco57I CTGAAG 1 cut(s) 236
Eco88I CYCGRG 1 cut(s) 341
EcoICRI GAGCTC 1 cut(s) 187
EcoNI CCTNNNNNAGG 2 cut(s) 280, 419
EcoO109I RGGNCCY 1 cut(s) 488
EcoRII CCWGG 2 cut(s) 275, 406
EcoT38I GRGCYC 1 cut(s) 189
EgeI GGCGCC 1 cut(s) 198
EheI GGCGCC 1 cut(s) 198
FaiI YATR 3 cut(s) 384, 428, 447
Fnu4HI GCNGC 2 cut(s) 224, 242
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 2 cut(s) 224, 242
GlaI GCGC 2 cut(s) 198, 450
GluI GCNGC 2 cut(s) 224, 242
GsaI CCCAGC 1 cut(s) 358
HaeII RGCGCY 1 cut(s) 200
HaeIII GGCC 1 cut(s) 275
HapII CCGG 3 cut(s) 200, 227, 316
HhaI GCGC 2 cut(s) 199, 451
Hin1I GRCGYC 1 cut(s) 197
Hin6I GCGC 2 cut(s) 197, 449
HinP1I GCGC 2 cut(s) 197, 449
HpaII CCGG 3 cut(s) 200, 227, 316
HphI GGTGA 4 cut(s) 10, 84, 109, 364
Hpy188I TCNGA 1 cut(s) 166
Hpy188III TCNNGA 2 cut(s) 129, 469
Hpy99I CGWCG 1 cut(s) 196
HpyAV CCTTC 2 cut(s) 93, 171
HpyCH4IV ACGT 1 cut(s) 107
HpyCH4V TGCA 2 cut(s) 267, 476
HpyF10VI GCNNNNNNNGC 4 cut(s) 220, 264, 346, 355
HpyF3I CTNAG 2 cut(s) 281, 452
HpySE526I ACGT 1 cut(s) 107
Hsp92I GRCGYC 1 cut(s) 197
HspAI GCGC 2 cut(s) 197, 449
KasI GGCGCC 1 cut(s) 196
LmnI GCTCC 2 cut(s) 184, 192
Lsp1109I GCAGC 1 cut(s) 210
LweI GCATC 1 cut(s) 276
MaeII ACGT 1 cut(s) 107
MboII GAAGA 6 cut(s) 25, 30, 117, 188, 229, 247
MhlI GDGCHC 1 cut(s) 189
Mly113I GGCGCC 1 cut(s) 197
MmeI TCCRAC 1 cut(s) 95
MroXI GAANNNNTTC 1 cut(s) 136
MslI CAYNNNNRTG 1 cut(s) 97
MspA1I CMGCKG 1 cut(s) 244
MspI CCGG 3 cut(s) 200, 227, 316
MspR9I CCNGG 3 cut(s) 277, 317, 408
Mva1269I GAATGC 1 cut(s) 478
MvaI CCWGG 2 cut(s) 277, 408
MwoI GCNNNNNNNGC 4 cut(s) 220, 264, 346, 355
NarI GGCGCC 1 cut(s) 197
NciI CCSGG 1 cut(s) 317
NlaIV GGNNCC 2 cut(s) 198, 490
NspV TTCGAA 1 cut(s) 155
PceI AGGCCT 1 cut(s) 275
PcsI WCGNNNNNNNCGW 2 cut(s) 152, 372
PctI GAATGC 1 cut(s) 478
PdmI GAANNNNTTC 1 cut(s) 136
PkrI GCNGC 2 cut(s) 225, 243
PluTI GGCGCC 1 cut(s) 200
PpuMI RGGWCCY 1 cut(s) 488
Psp124BI GAGCTC 1 cut(s) 189
Psp5II RGGWCCY 1 cut(s) 488
Psp6I CCWGG 2 cut(s) 275, 406
PspFI CCCAGC 1 cut(s) 354
PspGI CCWGG 2 cut(s) 275, 406
PspN4I GGNNCC 2 cut(s) 198, 490
PspPI GGNCC 3 cut(s) 313, 404, 488
PspPPI RGGWCCY 1 cut(s) 488
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
RseI CAYNNNNRTG 1 cut(s) 97
SacI GAGCTC 1 cut(s) 189
SatI GCNGC 2 cut(s) 224, 242
Sau96I GGNCC 3 cut(s) 313, 404, 488
ScrFI CCNGG 3 cut(s) 277, 317, 408
SduI GDGCHC 1 cut(s) 189
SetI ASST 6 cut(s) 110, 189, 225, 360, 392, 417
SfaNI GCATC 1 cut(s) 276
SfoI GGCGCC 1 cut(s) 198
SfuI TTCGAA 1 cut(s) 155
SinI GGWCC 3 cut(s) 313, 404, 488
SmiMI CAYNNNNRTG 1 cut(s) 97
SseBI AGGCCT 1 cut(s) 275
SsiI CCGC 4 cut(s) 6, 170, 242, 320
SspDI GGCGCC 1 cut(s) 196
SstI GAGCTC 1 cut(s) 189
StuI AGGCCT 1 cut(s) 275
StyD4I CCNGG 3 cut(s) 275, 315, 406
TaiI ACGT 1 cut(s) 110
TaqI TCGA 4 cut(s) 155, 270, 366, 468
TaqII GACCGA 1 cut(s) 130
TauI GCSGC 1 cut(s) 244
TscAI CASTG 1 cut(s) 483
TseI GCWGC 1 cut(s) 223
TspDTI ATGAA 1 cut(s) 16
TspRI CASTG 1 cut(s) 483
VpaK11BI GGWCC 3 cut(s) 313, 404, 488
XagI CCTNNNNNAGG 2 cut(s) 280, 419
XmnI GAANNNNTTC 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.