Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005383

Physical Location & Seq

Genomic Coordinates Reverse (-)

22430105 .. 22435134

Gene Structure 5030 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G55310.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2754 bp

ATGGCCACTGAGGCGTTTGTGAAACCCGTTGTACCAAATGGTCATGACGGGTACGAAGATGAAGATGAAGATGACATTCCTTTGGTTTTTAAGAGAAACAGTAATACAGCAGCTACAACAAATCGTCCTAGCCCTATTAATAATGCTATGAGAAACTCGGCTATTGGTTCCACCAAGTCCTCACCACCAATGAGGTCTCCTTTAACAAGCCCGAATAGGTCAGCTTCTTCATCCACTAGAAGTTCAATGATGAAACCAGCATTACCGTCATCTTCATCTGTTCAACGTTCGACTCTGAAGTCACCGTTGCGAGATGATAGATCTGTTGTTGCTAAGGAGAGGAACGGTTTTGGGAAAGCTCCTTCTGTGTCTAAAAGTGATGATGAGGATTCTGAGGATGATAAACCATTGAGTGCTAGGCTTAAATTGGATTCTAAAGAGGTTACCAAACAGCCAAGCTCTTCAGGACGTGGGAGTACGCAGCAAGCAGTACAAAAGAGCAACATGAGGCCTCAGGGATTGAGTGATTACACTAAAAAGAAAGTTTTGGATGAGAGAGCTCCCATGTCCTCAACGGTTCAGACAAAGACCAGTGTGGGTACATCAAGTAGCAAGCCTGTTCATATTGAACAGAAGAGACCTTTGGTTAACAATATTGATCGGAATGGTTTGAAACCTAAGACTGAAGGGCACAGTTCTCAGGCACCTGCTAAGAGACCTCTTGAAAAGGGGAGTTCTTCGAATCAATCTTCTGTTAAGAGGCCCAAGCTGTCAGAGCCAGCTAGACCAGTGAAAGTGGAGCAAGGTTCACATATTTCAGCTACGCAAGATGCCAAAGGAAAGAATCTGGATGCATCGAAGCCGTTGAGAGCTAACCAAGCAACTGTTAAAGAAGATAACTCAGATGGTGATGATCATGTTCCTATTGCCTCAAGGATGAAGTCAGATTCCTCAAATAATAAATCGTCTTCCGCAAAGCCAAGTTCTAGTAAAATGATAGCGTCTTCATCCAGAACAATAGCTCAAAAGCCCAACAAATGGGTGAAAGATTCTAAATATTCGAAATCTTCTAAATCATTACCTTCTGGAGATGGGCAAAAGAAATGGAAAACTCTACAGCACAATGGTGTTATTTTTCCACCTCCATATAAGCGTCATGGGGTTAAGATATTGTTCCAGGGAAAGCCTGTTGACTTAACTCCTGAACAAGAAGAGGTTGCAACTATGTTTGCGGTGATGAGGGAAACAGAATATTATAAAAAACCAAAGTTCAGAGAGAATTTCTGGAATGATTGGCGGAAACTACTTGGAAAGAACCATATGATTAAAAGTTTAGATGATTGTGACTTCACTCCCATATATGAATGGTATATGCAGGAGAAGGAGACAAAGAAACAAATGACTGCAGAAGAGAAAAGGATTGTGAAAGAGGAGAAATTAAAGCAGGAGGAGAAATATATGTGGGCTGTTCTTGATGGTGTCAGGGAGAGGATTGGAAATTTCAGAGTTGAGCCCCCTGGGTTGTTTCGAGGCCGGGGAGAACATCCCAAGATGGGGAAACTGAAAAAGCGGATTCGTCCTTGTGATATTACAATCAACATTGGTAAAGAGGCGCCTATTCCAGAATGCCCTATCCCTGGTGAAAGATGGAAAGAAGTTAAGCATGACAATACTGTTACCTGGCTTGCTTTTTGGAGTGATCCTATCAATCCAAAAGAGTTCAAGTATGTATTCTTGGCAGCTAGCAGTTCGTTGAAGGGACAAAGCGACAAAGAGAAGTATGAGAAAGCGAGGAAGCTACATAACCATATAGGGAGTATTAGAGCAGCATACACCAAAGATTTTAATAACAAGGATGTAACAAAGCGGCAAATAGCAGTGGCAACATATCTCATCGATAAGCTAGCCCTTAGGGCTGGAAATGAGAAGGATGACGATGAGGCAGATACTGTTGGTTGTTGTACATTGAAAGTAGGAAACGTGGAGTGTATTCCTCCAAATAAGTTAAAGTTTGACTTCCTTGGTAAAGACTCTATTCAGTATGTAAACACAGTTGAGGTTGAGCCTCTGGTGTATAAGGCAATTGGGCAGTTCCAAGCAGGAAAATCGAAAACCGATGATCTCTTTGATGAGCTAGATACTTCAAAACTCAATACTCATCTTAAGGAGCTTATGGCTGGTCTCACAGCCAAAGTATTCCGTACGTATAATGCATCCATCACATTGGATTTAATGTTGAGTAAAGAAACCAGAGACGGTGACGTTCCTGAAAAAGTGGTGGTTTATCAGCAAGCGAACAAGGAGGTTGCTATCATTTGTAACCATCAACGTACTGTCTCGAAATCTCACGGAGCACAAGTGGAGAAGCTGGCTGTGAAAATAGAAGAACTAAGGGAGCAAATAAAAGAGCTGAATATTGATCTCGACAGGGCTAAGAAAGGAAGAACACCGTTAATGGGATCTGATGGAAAGAGAAAGAGGAACTTAACACCTGAAGCATTGGAGAAGAAGATAATGCAAACGCAAGGTAAGATCGAGAAGATGGAAAGAGATATGCAAACAAAAGAAGATATGAAAACTGTCGCATTGGGCACATCTAAGATCAACTACATGGATCCTAGGATCACTGTTGCGTGGTGCAAACGACATGATGTCCCCATTGAGAAGATATTTAACAAGTCTCTTCTGGCTAAGTTCGCTTGGGCAATGGATGTTGATCCTGAGTTCAGATTCTGTTCAAGTACCGATGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0003674 GO:0003824 GO:0003916 GO:0003917 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0005730 GO:0006139 GO:0006259 GO:0006260 GO:0006265 GO:0006325 GO:0006338 GO:0006725 GO:0006807 GO:0006996 GO:0007059 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016020 GO:0016043 GO:0016853 GO:0031298 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0051276 GO:0070013 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0140097 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

917

Amino Acids

103.05

Weight (kDa)

9.48

Isoelectric Point (pI)

45.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Topoisom_I_N	PF02919	367 - 579	3.2e-92	Eukaryotic DNA topoisomerase I, DNA binding fragment
Topoisom_I	PF01028	582 - 805	8.1e-95	Eukaryotic DNA topoisomerase I, catalytic core
Topo_C_assoc	PF14370	844 - 911	3.1e-35	C-terminal topoisomerase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005383)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G55310
fragaria_vesca	FvH4_4g16730 FvH4_4g16730
malus_domestica	MD00G1109500.v1.1 MD13G1176900.v1.1 MD16G1178600.v1.1
prunus_persica	Prupe.1G146000_v2.0.a1 Prupe.1G146000_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1
pyrus_communis	pycom10g21080 pycom13g15260 pycom16g15010
rosa_chinensis	RchiOBHm_Chr4g0420301
rosa_laevigata	RLG00000003998 RLG00000007777 RLG00000007780
rosa_multiflora	Rmu_sc0001767.1_g000005
rosa_roxburghii	Rroxscaffold_5G00362940
rosa_rugosa	Rorug04G0163000 Rorug04G0163100 Rorug04G0163200
rosa_samantha	Rh4AG224100 Rh4BG227100 Rh4CG238200
rosa_wichuraiana	Rw4G019300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1257
AarI	CACCTGC	1 cut(s)	715
AasI	GACNNNNNNGTC	1 cut(s)	298
Acc36I	ACCTGC	1 cut(s)	715
AccB1I	GGYRCC	2 cut(s)	703, 1612
AccB7I	CCANNNNNTGG	1 cut(s)	1038
AciI	CCGC	5 cut(s)	972, 1232, 1297, 1570, 1867
AclI	AACGTT	1 cut(s)	286
AclWI	GGATC	6 cut(s)	1694, 2467, 2609, 2622, 2630, 2711
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	2 cut(s)	1279, 1498
AcuI	CTGAAG	4 cut(s)	317, 447, 705, 2514
AcyI	GRCGYC	1 cut(s)	1613
AfaI	GTAC	9 cut(s)	33, 53, 478, 492, 601, 1963, 2203, 2332, 2743
AfiI	CCNNNNNNNGG	5 cut(s)	1038, 1553, 1554, 1637, 2456
AflII	CTTAAG	1 cut(s)	2162
AhdI	GACNNNNNGTC	1 cut(s)	2651
AjiI	CACGTC	1 cut(s)	470
AjnI	CCWGG	4 cut(s)	1176, 1516, 1636, 1679
AjuI	GAANNNNNNNTTGG	2 cut(s)	626, 658
AleI	CACNNNNGTG	1 cut(s)	1125
Alw21I	GWGCWC	2 cut(s)	562, 2356
Alw26I	GTCTC	8 cut(s)	201, 631, 709, 1379, 2186, 2247, 2341, 2685
AlwI	GGATC	6 cut(s)	1694, 2467, 2609, 2622, 2630, 2711
AlwNI	CAGNNNCTG	2 cut(s)	1949, 2733
AoxI	GGCC	4 cut(s)	3, 509, 761, 1531
ApeKI	GCWGC	4 cut(s)	110, 481, 1739, 1826
ApoI	RAATTY	2 cut(s)	1279, 1498
AseI	ATTAAT	1 cut(s)	138
AspA2I	CCTAGG	1 cut(s)	2618
AspLEI	GCGC	1 cut(s)	1615
AspS9I	GGNCC	1 cut(s)	762
AsuC2I	CCSGG	1 cut(s)	1535
AsuHPI	GGTGA	7 cut(s)	174, 294, 920, 1054, 1246, 1652, 2270
AsuII	TTCGAA	2 cut(s)	740, 1061
AsuNHI	GCTAGC	2 cut(s)	1742, 1903
AvrII	CCTAGG	1 cut(s)	2618
AxyI	CCTNAGG	2 cut(s)	513, 1910
BaeGI	GKGCMC	2 cut(s)	693, 2594
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	2614
BanI	GGYRCC	2 cut(s)	703, 1612
BanII	GRGCYC	2 cut(s)	562, 1515
BbsI	GAAGAC	2 cut(s)	960, 996
Bbv12I	GWGCWC	2 cut(s)	562, 2356
BbvI	GCAGC	4 cut(s)	122, 493, 1751, 1838
BccI	CCATC	9 cut(s)	899, 1085, 1469, 1546, 1641, 2225, 2331, 2459, 2536
BceAI	ACGGC	1 cut(s)	847
BcgI	CGANNNNNNTGC	2 cut(s)	2088, 2122
BciT130I	CCWGG	4 cut(s)	1178, 1518, 1638, 1681
BclI	TGATCA	1 cut(s)	913
BcnI	CCSGG	1 cut(s)	1535
BcoDI	GTCTC	8 cut(s)	201, 631, 709, 1379, 2186, 2247, 2341, 2685
BfaI	CTAG	9 cut(s)	129, 237, 417, 783, 987, 1743, 1904, 2135, 2619
BfmI	CTRYAG	2 cut(s)	1115, 1404
BfoI	RGCGCY	1 cut(s)	1616
BfrI	CTTAAG	1 cut(s)	2162
BfuAI	ACCTGC	1 cut(s)	715
BglI	GCCNNNNNGGC	2 cut(s)	11, 1913
BglII	AGATCT	1 cut(s)	320
BisI	GCNGC	5 cut(s)	111, 482, 1740, 1827, 1868
BlnI	CCTAGG	1 cut(s)	2618
BlsI	GCNGC	5 cut(s)	112, 483, 1741, 1828, 1869
Bme1390I	CCNGG	5 cut(s)	1178, 1518, 1535, 1638, 1681
BmeRI	GACNNNNNGTC	1 cut(s)	2651
BmgBI	CACGTC	1 cut(s)	470
BmgT120I	GGNCC	1 cut(s)	762
BmiI	GGNNCC	4 cut(s)	169, 705, 1614, 2616
BmrFI	CCNGG	5 cut(s)	1178, 1518, 1535, 1638, 1681
BmsI	GCATC	4 cut(s)	820, 841, 863, 2222
BmtI	GCTAGC	2 cut(s)	1746, 1907
BpiI	GAAGAC	2 cut(s)	960, 996
BpmI	CTGGAG	1 cut(s)	1107
Bpu10I	CCTNAGC	1 cut(s)	333
Bpu14I	TTCGAA	2 cut(s)	740, 1061
BpuEI	CTTGAG	1 cut(s)	916
BpuMI	CCSGG	1 cut(s)	1535
Bsa29I	ATCGAT	1 cut(s)	1896
BsaAI	YACGTR	1 cut(s)	2205
BsaBI	GATNNNNATC	1 cut(s)	2715
BsaHI	GRCGYC	1 cut(s)	1613
BsaI	GGTCTC	4 cut(s)	201, 631, 709, 2186
BsaJI	CCNNGG	7 cut(s)	1177, 1516, 1517, 1534, 1636, 2020, 2618
BsaXI	ACNNNNNCTCC	2 cut(s)	2343, 2373
Bsc4I	CCNNNNNNNGG	5 cut(s)	1038, 1553, 1554, 1637, 2456
Bse1I	ACTGG	2 cut(s)	591, 788
Bse21I	CCTNAGG	2 cut(s)	513, 1910
Bse3DI	GCAATG	1 cut(s)	2712
Bse8I	GATNNNNATC	1 cut(s)	2715
BseBI	CCWGG	4 cut(s)	1178, 1518, 1638, 1681
BseCI	ATCGAT	1 cut(s)	1896
BseDI	CCNNGG	7 cut(s)	1177, 1516, 1517, 1534, 1636, 2020, 2618
BseJI	GATNNNNATC	1 cut(s)	2715
BseLI	CCNNNNNNNGG	5 cut(s)	1038, 1553, 1554, 1637, 2456
BseMI	GCAATG	1 cut(s)	2712
BseMII	CTCAG	5 cut(s)	384, 527, 713, 915, 2712
BseNI	ACTGG	2 cut(s)	591, 788
BseRI	GAGGAG	2 cut(s)	1445, 1463
BseSI	GKGCMC	2 cut(s)	693, 2594
BseXI	GCAGC	4 cut(s)	122, 493, 1751, 1838
BshFI	GGCC	4 cut(s)	5, 511, 763, 1533
BshNI	GGYRCC	2 cut(s)	703, 1612
BshVI	ATCGAT	1 cut(s)	1896
BsiHKAI	GWGCWC	2 cut(s)	562, 2356
BsiSI	CCGG	1 cut(s)	1534
BsiWI	CGTACG	1 cut(s)	2201
BslFI	GGGAC	2 cut(s)	1773, 2639
BslI	CCNNNNNNNGG	5 cut(s)	1038, 1553, 1554, 1637, 2456
BsmAI	GTCTC	8 cut(s)	201, 631, 709, 1379, 2186, 2247, 2341, 2685
BsmBI	CGTCTC	1 cut(s)	2247
BsmFI	GGGAC	2 cut(s)	1773, 2639
BsmI	GAATGC	1 cut(s)	1631
BsnI	GGCC	4 cut(s)	5, 511, 763, 1533
Bso31I	GGTCTC	4 cut(s)	201, 631, 709, 2186
Bsp119I	TTCGAA	2 cut(s)	740, 1061
Bsp1286I	GDGCHC	5 cut(s)	562, 693, 1515, 2356, 2594
Bsp1407I	TGTACA	1 cut(s)	1961
BspACI	CCGC	5 cut(s)	972, 1232, 1297, 1570, 1867
BspANI	GGCC	4 cut(s)	5, 511, 763, 1533
BspCNI	CTCAG	5 cut(s)	385, 526, 712, 914, 2713
BspDI	ATCGAT	1 cut(s)	1896
BspHI	TCATGA	1 cut(s)	43
BspLI	GGNNCC	4 cut(s)	169, 705, 1614, 2616
BspMAI	CTGCAG	1 cut(s)	1408
BspMI	ACCTGC	1 cut(s)	715
BspOI	GCTAGC	2 cut(s)	1746, 1907
BspPI	GGATC	6 cut(s)	1694, 2467, 2609, 2622, 2630, 2711
BspQI	GCTCTTC	1 cut(s)	466
BspT104I	TTCGAA	2 cut(s)	740, 1061
BspT107I	GGYRCC	2 cut(s)	703, 1612
BspTI	CTTAAG	1 cut(s)	2162
BspTNI	GGTCTC	4 cut(s)	201, 631, 709, 2186
BsrDI	GCAATG	1 cut(s)	2712
BsrGI	TGTACA	1 cut(s)	1961
BsrI	ACTGG	2 cut(s)	591, 788
BssECI	CCNNGG	7 cut(s)	1177, 1516, 1517, 1534, 1636, 2020, 2618
BssNI	GRCGYC	1 cut(s)	1613
BssT1I	CCWWGG	2 cut(s)	2020, 2618
Bst2UI	CCWGG	4 cut(s)	1178, 1518, 1638, 1681
Bst6I	CTCTTC	5 cut(s)	466, 629, 1206, 1404, 2688
BstACI	GRCGYC	1 cut(s)	1613
BstAFI	CTTAAG	1 cut(s)	2162
BstAUI	TGTACA	1 cut(s)	1961
BstBAI	YACGTR	1 cut(s)	2205
BstBI	TTCGAA	2 cut(s)	740, 1061
BstC8I	GCNNGC	8 cut(s)	486, 614, 780, 1686, 1744, 1905, 2292, 2370
BstEII	GGTNACC	1 cut(s)	442
BstH2I	RGCGCY	1 cut(s)	1616
BstHHI	GCGC	1 cut(s)	1615
BstMAI	GTCTC	8 cut(s)	201, 631, 709, 1379, 2186, 2247, 2341, 2685
BstMWI	GCNNNNNNNGC	5 cut(s)	11, 775, 878, 1913, 2696
BstNI	CCWGG	4 cut(s)	1178, 1518, 1638, 1681
BstPI	GGTNACC	1 cut(s)	442
BstSCI	CCNGG	5 cut(s)	1176, 1516, 1533, 1636, 1679
BstSFI	CTRYAG	2 cut(s)	1115, 1404
BstSLI	GKGCMC	2 cut(s)	693, 2594
BstSNI	TACGTA	1 cut(s)	2205
BstV1I	GCAGC	4 cut(s)	122, 493, 1751, 1838
BstV2I	GAAGAC	2 cut(s)	960, 996
BstX2I	RGATCY	3 cut(s)	320, 2459, 2614
BstXI	CCANNNNNNTGG	1 cut(s)	2224
BstYI	RGATCY	3 cut(s)	320, 2459, 2614
Bsu15I	ATCGAT	1 cut(s)	1896
Bsu36I	CCTNAGG	2 cut(s)	513, 1910
BsuRI	GGCC	4 cut(s)	5, 511, 763, 1533
BsuTUI	ATCGAT	1 cut(s)	1896
BtrI	CACGTC	1 cut(s)	470
BtsI	GCAGTG	1 cut(s)	1884
BtsIMutI	CAGTG	5 cut(s)	6, 598, 795, 1884, 2625
BveI	ACCTGC	1 cut(s)	715
Cac8I	GCNNGC	8 cut(s)	486, 614, 780, 1686, 1744, 1905, 2292, 2370
CaiI	CAGNNNCTG	2 cut(s)	1949, 2733
CciI	TCATGA	1 cut(s)	43
CfoI	GCGC	1 cut(s)	1615
Cfr13I	GGNCC	1 cut(s)	762
ClaI	ATCGAT	1 cut(s)	1896
CseI	GACGC	2 cut(s)	990, 1142
Csp6I	GTAC	9 cut(s)	32, 52, 477, 491, 600, 1962, 2202, 2331, 2742
CviAII	CATG	8 cut(s)	44, 505, 565, 917, 1157, 1664, 2611, 2648
CviQI	GTAC	9 cut(s)	32, 52, 477, 491, 600, 1962, 2202, 2331, 2742
DinI	GGCGCC	1 cut(s)	1614
DrdI	GACNNNNNNGTC	1 cut(s)	298
DriI	GACNNNNNGTC	1 cut(s)	2651
DseDI	GACNNNNNNGTC	1 cut(s)	298
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	5 cut(s)	466, 629, 1206, 1404, 2688
Eam1105I	GACNNNNNGTC	1 cut(s)	2651
EarI	CTCTTC	5 cut(s)	466, 629, 1206, 1404, 2688
EciI	GGCGGA	1 cut(s)	1312
Ecl136II	GAGCTC	1 cut(s)	560
Eco105I	TACGTA	1 cut(s)	2205
Eco130I	CCWWGG	2 cut(s)	2020, 2618
Eco147I	AGGCCT	1 cut(s)	511
Eco24I	GRGCYC	2 cut(s)	562, 1515
Eco31I	GGTCTC	4 cut(s)	201, 631, 709, 2186
Eco53kI	GAGCTC	1 cut(s)	560
Eco57I	CTGAAG	4 cut(s)	317, 447, 705, 2514
Eco81I	CCTNAGG	2 cut(s)	513, 1910
Eco91I	GGTNACC	1 cut(s)	442
EcoICRI	GAGCTC	1 cut(s)	560
EcoO65I	GGTNACC	1 cut(s)	442
EcoRII	CCWGG	4 cut(s)	1176, 1516, 1636, 1679
EcoT14I	CCWWGG	2 cut(s)	2020, 2618
EcoT22I	ATGCAT	2 cut(s)	856, 2215
EcoT38I	GRGCYC	2 cut(s)	562, 1515
EgeI	GGCGCC	1 cut(s)	1614
EheI	GGCGCC	1 cut(s)	1614
ErhI	CCWWGG	2 cut(s)	2020, 2618
Esp3I	CGTCTC	1 cut(s)	2247
FaeI	CATG	8 cut(s)	47, 508, 568, 920, 1160, 1667, 2614, 2651
FalI	AAGNNNNNCTT	4 cut(s)	2000, 2032, 2468, 2500
FaqI	GGGAC	2 cut(s)	1773, 2639
FatI	CATG	8 cut(s)	43, 504, 564, 916, 1156, 1663, 2610, 2647
FauNDI	CATATG	1 cut(s)	1320
FbaI	TGATCA	1 cut(s)	913
Fnu4HI	GCNGC	5 cut(s)	111, 482, 1740, 1827, 1868
FriOI	GRGCYC	2 cut(s)	562, 1515
Fsp4HI	GCNGC	5 cut(s)	111, 482, 1740, 1827, 1868
FspBI	CTAG	9 cut(s)	129, 237, 417, 783, 987, 1743, 1904, 2135, 2619
GlaI	GCGC	1 cut(s)	1614
GluI	GCNGC	5 cut(s)	111, 482, 1740, 1827, 1868
GsuI	CTGGAG	1 cut(s)	1107
HaeII	RGCGCY	1 cut(s)	1616
HaeIII	GGCC	4 cut(s)	5, 511, 763, 1533
HapII	CCGG	1 cut(s)	1534
HgaI	GACGC	2 cut(s)	990, 1142
HhaI	GCGC	1 cut(s)	1615
Hin1I	GRCGYC	1 cut(s)	1613
Hin1II	CATG	8 cut(s)	47, 508, 568, 920, 1160, 1667, 2614, 2651
Hin6I	GCGC	1 cut(s)	1613
HinP1I	GCGC	1 cut(s)	1613
HincII	GTYRAC	2 cut(s)	649, 1192
HindII	GTYRAC	2 cut(s)	649, 1192
HpaI	GTTAAC	1 cut(s)	649
HpaII	CCGG	1 cut(s)	1534
HphI	GGTGA	7 cut(s)	174, 294, 920, 1054, 1246, 1652, 2270
Hpy166II	GTNNAC	4 cut(s)	649, 809, 1192, 2047
Hpy8I	GTNNAC	4 cut(s)	649, 809, 1192, 2047
HpyAV	CCTTC	6 cut(s)	372, 680, 1092, 1375, 1750, 1921
HpyCH4IV	ACGT	6 cut(s)	286, 469, 1980, 2204, 2262, 2329
HpyCH4V	TGCA	8 cut(s)	854, 1220, 1375, 1406, 2213, 2518, 2557, 2640
HpyF10VI	GCNNNNNNNGC	5 cut(s)	11, 775, 878, 1913, 2696
HpySE526I	ACGT	6 cut(s)	286, 469, 1980, 2204, 2262, 2329
Hsp92I	GRCGYC	1 cut(s)	1613
Hsp92II	CATG	8 cut(s)	47, 508, 568, 920, 1160, 1667, 2614, 2651
HspAI	GCGC	1 cut(s)	1613
KasI	GGCGCC	1 cut(s)	1612
Ksp22I	TGATCA	1 cut(s)	913
KspAI	GTTAAC	1 cut(s)	649
LguI	GCTCTTC	1 cut(s)	466
LmnI	GCTCC	6 cut(s)	364, 565, 799, 2167, 2351, 2395
Lsp1109I	GCAGC	4 cut(s)	122, 493, 1751, 1838
LweI	GCATC	4 cut(s)	820, 841, 863, 2222
MaeI	CTAG	9 cut(s)	129, 237, 417, 783, 987, 1743, 1904, 2135, 2619
MaeII	ACGT	6 cut(s)	286, 469, 1980, 2204, 2262, 2329
MaeIII	GTNAC	7 cut(s)	300, 442, 1343, 1675, 1858, 2258, 2318
MfeI	CAATTG	1 cut(s)	2082
MflI	RGATCY	3 cut(s)	320, 2459, 2614
MhlI	GDGCHC	5 cut(s)	562, 693, 1515, 2356, 2594
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	5 cut(s)	425, 1279, 1436, 1498, 2082
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	1 cut(s)	1613
MlyI	GAGTC	2 cut(s)	286, 2024
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	2 cut(s)	856, 2215
MscI	TGGCCA	1 cut(s)	5
MslI	CAYNNNNRTG	1 cut(s)	1125
Msp20I	TGGCCA	1 cut(s)	5
MspCI	CTTAAG	1 cut(s)	2162
MspI	CCGG	1 cut(s)	1534
MspR9I	CCNGG	5 cut(s)	1178, 1518, 1535, 1638, 1681
MunI	CAATTG	1 cut(s)	2082
Mva1269I	GAATGC	1 cut(s)	1631
MvaI	CCWGG	4 cut(s)	1178, 1518, 1638, 1681
MwoI	GCNNNNNNNGC	5 cut(s)	11, 775, 878, 1913, 2696
NarI	GGCGCC	1 cut(s)	1613
NciI	CCSGG	1 cut(s)	1535
NdeI	CATATG	1 cut(s)	1320
NheI	GCTAGC	2 cut(s)	1742, 1903
NlaIII	CATG	8 cut(s)	47, 508, 568, 920, 1160, 1667, 2614, 2651
NlaIV	GGNNCC	4 cut(s)	169, 705, 1614, 2616
NmeAIII	GCCGAG	1 cut(s)	137
NmuCI	GTSAC	3 cut(s)	300, 1343, 2258
NsiI	ATGCAT	2 cut(s)	856, 2215
NspV	TTCGAA	2 cut(s)	740, 1061
OliI	CACNNNNGTG	1 cut(s)	1125
PagI	TCATGA	1 cut(s)	43
PaqCI	CACCTGC	1 cut(s)	715
PasI	CCCWGGG	1 cut(s)	1517
PceI	AGGCCT	1 cut(s)	511
PciSI	GCTCTTC	1 cut(s)	466
PctI	GAATGC	1 cut(s)	1631
PfeI	GAWTC	8 cut(s)	389, 431, 742, 844, 947, 1049, 1573, 2730
Pfl23II	CGTACG	1 cut(s)	2201
PflMI	CCANNNNNTGG	1 cut(s)	1038
PkrI	GCNGC	5 cut(s)	112, 483, 1741, 1828, 1869
PleI	GAGTC	2 cut(s)	286, 2024
PluTI	GGCGCC	1 cut(s)	1616
PpsI	GAGTC	2 cut(s)	286, 2024
Ppu21I	YACGTR	1 cut(s)	2205
PshBI	ATTAAT	1 cut(s)	138
PsiI	TTATAA	1 cut(s)	1257
Psp124BI	GAGCTC	1 cut(s)	562
Psp1406I	AACGTT	1 cut(s)	286
Psp6I	CCWGG	4 cut(s)	1176, 1516, 1636, 1679
PspEI	GGTNACC	1 cut(s)	442
PspGI	CCWGG	4 cut(s)	1176, 1516, 1636, 1679
PspLI	CGTACG	1 cut(s)	2201
PspN4I	GGNNCC	4 cut(s)	169, 705, 1614, 2616
PspPI	GGNCC	1 cut(s)	762
PstI	CTGCAG	1 cut(s)	1408
PstNI	CAGNNNCTG	2 cut(s)	1949, 2733
PsuI	RGATCY	3 cut(s)	320, 2459, 2614
RsaI	GTAC	9 cut(s)	33, 53, 478, 492, 601, 1963, 2203, 2332, 2743
RsaNI	GTAC	9 cut(s)	32, 52, 477, 491, 600, 1962, 2202, 2331, 2742
RseI	CAYNNNNRTG	1 cut(s)	1125
SacI	GAGCTC	1 cut(s)	562
SapI	GCTCTTC	1 cut(s)	466
SatI	GCNGC	5 cut(s)	111, 482, 1740, 1827, 1868
Sau96I	GGNCC	1 cut(s)	762
SchI	GAGTC	2 cut(s)	286, 2024
ScrFI	CCNGG	5 cut(s)	1178, 1518, 1535, 1638, 1681
SduI	GDGCHC	5 cut(s)	562, 693, 1515, 2356, 2594
SfaNI	GCATC	4 cut(s)	820, 841, 863, 2222
SfcI	CTRYAG	2 cut(s)	1115, 1404
SfoI	GGCGCC	1 cut(s)	1614
SfuI	TTCGAA	2 cut(s)	740, 1061
SmiMI	CAYNNNNRTG	1 cut(s)	1125
SmlI	CTYRAG	2 cut(s)	931, 2162
SmoI	CTYRAG	2 cut(s)	931, 2162
SnaBI	TACGTA	1 cut(s)	2205
Sse9I	AATT	5 cut(s)	425, 1279, 1436, 1498, 2082
SseBI	AGGCCT	1 cut(s)	511
SsiI	CCGC	5 cut(s)	972, 1232, 1297, 1570, 1867
SspDI	GGCGCC	1 cut(s)	1612
SspI	AATATT	4 cut(s)	655, 1058, 1253, 2416
SspMI	CTAG	9 cut(s)	129, 237, 417, 783, 987, 1743, 1904, 2135, 2619
SstI	GAGCTC	1 cut(s)	562
StuI	AGGCCT	1 cut(s)	511
StyD4I	CCNGG	5 cut(s)	1176, 1516, 1533, 1636, 1679
StyI	CCWWGG	2 cut(s)	2020, 2618
TaiI	ACGT	6 cut(s)	289, 472, 1983, 2207, 2265, 2332
TasI	AATT	5 cut(s)	425, 1279, 1436, 1498, 2082
TatI	WGTACW	2 cut(s)	490, 1961
TauI	GCSGC	1 cut(s)	1870
TfiI	GAWTC	8 cut(s)	389, 431, 742, 844, 947, 1049, 1573, 2730
TscAI	CASTG	5 cut(s)	13, 598, 795, 1884, 2632
TseFI	GTSAC	3 cut(s)	300, 1343, 2258
TseI	GCWGC	4 cut(s)	110, 481, 1739, 1826
Tsp45I	GTSAC	3 cut(s)	300, 1343, 2258
TspGWI	ACGGA	2 cut(s)	2189, 2364
TspRI	CASTG	5 cut(s)	13, 598, 795, 1884, 2632
Van91I	CCANNNNNTGG	1 cut(s)	1038
Vha464I	CTTAAG	1 cut(s)	2162
VspI	ATTAAT	1 cut(s)	138
XapI	RAATTY	2 cut(s)	1279, 1498
XmaJI	CCTAGG	1 cut(s)	2618
XspI	CTAG	9 cut(s)	129, 237, 417, 783, 987, 1743, 1904, 2135, 2619
Zsp2I	ATGCAT	2 cut(s)	856, 2215

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.