Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0005383

Physical Location & Seq

Genomic Coordinates Reverse (-)

15262809 .. 15270090

Gene Structure 7282 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1178600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2661 bp

ATGATGGCTGTTCAGACTTCTACTAAACTGAATTTATGTGAAGACGAGGATGATGAACCTTTAGTTTTCAAACGGTACAGCACAAAATCTAAACCGAATCAATTAAATGCTGAAGCAAATAAATTATCAGCTGAGAGGCAAGTTGGACAACCAGGGAAGCAGTTATCTGAGCACCGTTATTCCAATGGTCTAAACTCGAGTGTTCAAAAGGTTAAGATGGTTCCATCAACCAAATCACCACCAGTAAAATCTCCGCTAGCAAGCCCAAAAGCTTCAACTTCATCTGCTGGGGCATCACCAGTATCAAATTCAAAAGCTTCAACTTCATCTAGGGCATCACCAGTTTCCAAATCACCACCGTCGACTACATTAGATGATCAGTCAAAGCGGTTGTCAGAACAGAATAAGTCTACCACGATTAAGGAGGAAATTAATTCTATTGAACATCTTGCTCAAGGAAATAGTGATTCTGAAGATGAAAAACCGATAGGTGCCAGACTGGGGAAAGTGAAACCTTCTGAAGATTCGGATGATGATAAACCTTTATCATCAAGGATTGCACCAAAGTCCAAGGTGGGAACATCAGGCAGTTGGCCTTATGATTCTAACAAAAGGAAGCCCGTTGTTTCAAAAGTTCAGAAGAATGGTTCCAGCACAATAGATAAAGGCAAAGCTTCATCTGTGGTCTCTAGTAAGAGGCCTCTAGATAAGGTCAATTCTTCTGAGAACTCTTCAGCTAAAAGGCCAAAAGTTTCAGATTCTTCTACATCTGCAAAGAATAAGCAGGCATCAGCCAAAAAAGAACCAAAGGAAGAAGATGATGATGATGATGATGACTTCGTTCCAATTTCCCAGAGGAGTAAGAAAGTTACATCTGATAGTAAACCATCTCTGACAAAGCAAACGGTAACCAAAGTTGGTTCATCCTCAATCACGAAAACAATCAAGAAGGGCCAAAAAGCTTCAAAATTTTCAAAGTATTCTAAGTCGACAACAAAGCCACCGGGCTCTAATGATGGGCAGATAAAATGGACTACTTTAAAACACAATGGTGTCATTTTCCCGCCTCCTTACAACCCTCATGGGGTAAAGATGCTCTATAATGGGAAGCCAGTCACCTTGACTCCTGAACAAGAGGAGGTTGCAACAATGTATGCAGTGATGAAAGATACTGATTATGTGCAGAAAGAAACATTCAGAAACAATTTCTGGGCTGATTGGCATAAGTTGCTTGGGAAGAACCATGTAATTCAGAAATTGGATGCTTGTGATTTTACGCCAATATATGACTGGTATCAGAATGAAAAGGAAAAGAAGAAACAAATGAGCACGGAAGAGAAGAAGGCCTTGAAGGAGGAGAAACTGAAACAAGAGGAGAAGTATATGTGGGCTGAGGTTGATGGTGATAAGGAGAAGGTTGGAAACTTCAGAGTTGAACCACCTGGGTTATTTCGAGGCCGTGGAGAGCATCCAAAGTCGGGAAAGCTGAAAAGACGGATTCGTCCAAGTGATGTTACAATTAATATAGGAAAGGATGCCCCAATTCCAGAATGTCCCATCCCTGGTGAAAGCTGGAAAGAAGTAAGACATGACAATACGGTTACATGGTTAGCTTTCTGGAATGATCCAATTAATCAAAAGGAATTCAAATACGTATTTCTGGCACCTAGTAGTTCCTTGAAGGGGAAAAGTGACGAGCTGAAATATGATAAAGCAAGGAGATTGACGGACTGCATAGGAAAAATCAGGGCTTCATACACCAAGGATTTTACTAGTAAAGATGTATCTAAGCGCCAGATTGCGGTTGCTACCTATCTCATTGATAAACTTGCCCTCAGGGCGGGCAATGAGAAGGATGATGATGAAGCCGATACTGTTGGTTGCTGCACTTTAAAAGTAGAGAATGTAAAAGCAATCCCCCCTAACTCGTTGGAGTTCAACTTCCTTGGTAAAGATTCAATCAGATATGAAAATACTGTTGAAGTTGAGATTCCTGTTTACAAGGCAATTATGCAGTTCCAGTCTGGGAAAAGTGGCAGTGATGATCTCTTTGACTTGTTGGATACCAGTAGATTGAATGCTCATCTAAAGGAAAAAATGGATGGCCTTACAGCGAAAGTGTTTCGTACCTACAATGCATCTGTCACATTGGATAACATATTGAACAAGGAAACCAAAGATGGAGATCTTTCAGAGAAACTCGTTGTTTATCAGCATGCAAACAAAGAGGTCGCCATTATTTGTAATCATCAGCGTACTGTCTCAAAGTCTCACAGTGCACAAATGTCTAAGTTAACGGAGAAGATTGCTGAACTTCAGGGCAACCTGAAAGAGCTGAAAGTAGATTTGGACAGGGCCAAAAAAGGAAAGCCCCCACTGAAAGATGCTGATGGAAAGCAAAAGAAAAATTTGACCCCGGAAGCGTTGGAGAGGAAGATAGCTCAAACAAATGCAAAGATAGAAAAAATGGAACTGGATATGAGAATTAAAGAGGATCTGAAAACAGTGGCATTGGGAACGTCGAAAATCAACTATCTTGACCCTAGGATCACCGTTGCTTGGTGCAAGCGACATGAAGTTCCCATCGAGAAGATATTCAACAAGTCGCTCCTCGCAAAGTTTTCTTGGGCAATGCGAGTGGAGCCTGACTTCAGATTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

887

Amino Acids

99.09

Weight (kDa)

9.38

Isoelectric Point (pI)

38.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Topoisom_I_N	PF02919	342 - 554	6.5e-95	Eukaryotic DNA topoisomerase I, DNA binding fragment
Topoisom_I	PF01028	557 - 781	8.6e-93	Eukaryotic DNA topoisomerase I, catalytic core
Topo_C_assoc	PF14370	818 - 886	6.1e-35	C-terminal topoisomerase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005383)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G55310
fragaria_vesca	FvH4_4g16730 FvH4_4g16730
malus_domestica	MD00G1109500.v1.1 MD13G1176900.v1.1 MD16G1178600.v1.1
prunus_persica	Prupe.1G146000_v2.0.a1 Prupe.1G146000_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1
pyrus_communis	pycom10g21080 pycom13g15260 pycom16g15010
rosa_chinensis	RchiOBHm_Chr4g0420301
rosa_laevigata	RLG00000003998 RLG00000007777 RLG00000007780
rosa_multiflora	Rmu_sc0001767.1_g000005
rosa_roxburghii	Rroxscaffold_5G00362940
rosa_rugosa	Rorug04G0163000 Rorug04G0163100 Rorug04G0163200
rosa_samantha	Rh4AG224100 Rh4BG227100 Rh4CG238200
rosa_wichuraiana	Rw4G019300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1500
AccB1I	GGYRCC	2 cut(s)	491, 1663
AccI	GTMKAC	3 cut(s)	362, 410, 989
AciI	CCGC	5 cut(s)	254, 388, 1064, 1802, 1841
AclWI	GGATC	3 cut(s)	1619, 2502, 2555
AcsI	RAATTY	5 cut(s)	31, 307, 968, 1643, 2407
AcuI	CTGAAG	7 cut(s)	132, 492, 540, 717, 1411, 2300, 2635
AfaI	GTAC	3 cut(s)	77, 2128, 2257
AfiI	CCNNNNNNNGG	8 cut(s)	501, 1084, 1478, 1562, 1683, 1801, 1840, 2559
AhlI	ACTAGT	1 cut(s)	1772
AjnI	CCWGG	3 cut(s)	151, 1441, 1561
AjuI	GAANNNNNNNTTGG	2 cut(s)	2330, 2362
AleI	CACNNNNGTG	1 cut(s)	1050
Alw21I	GWGCWC	3 cut(s)	174, 1331, 2281
Alw26I	GTCTC	3 cut(s)	691, 2266, 2274
Alw44I	GTGCAC	1 cut(s)	2277
AlwI	GGATC	3 cut(s)	1619, 2502, 2555
AlwNI	CAGNNNCTG	1 cut(s)	2658
Ama87I	CYCGRG	1 cut(s)	196
AoxI	GGCC	8 cut(s)	593, 698, 743, 952, 1344, 1456, 2104, 2355
ApaLI	GTGCAC	1 cut(s)	2277
ApeKI	GCWGC	1 cut(s)	1884
ApoI	RAATTY	5 cut(s)	31, 307, 968, 1643, 2407
ArsI	GACNNNNNNTTYG	2 cut(s)	377, 409
AseI	ATTAAT	3 cut(s)	432, 1521, 1632
Asp700I	GAANNNNTTC	1 cut(s)	2594
AspA2I	CCTAGG	1 cut(s)	2543
AspLEI	GCGC	1 cut(s)	1794
AspS9I	GGNCC	2 cut(s)	952, 2355
AsuC2I	CCSGG	2 cut(s)	1005, 2417
AsuHPI	GGTGA	8 cut(s)	228, 288, 330, 345, 1108, 1415, 1577, 2542
AsuNHI	GCTAGC	1 cut(s)	256
AvaI	CYCGRG	1 cut(s)	196
AvrII	CCTAGG	1 cut(s)	2543
AxyI	CCTNAGG	1 cut(s)	1835
BaeGI	GKGCMC	1 cut(s)	2281
BanI	GGYRCC	2 cut(s)	491, 1663
BanII	GRGCYC	1 cut(s)	1010
BbsI	GAAGAC	1 cut(s)	48
Bbv12I	GWGCWC	3 cut(s)	174, 1331, 2281
BbvCI	CCTCAGC	1 cut(s)	1392
BbvI	GCAGC	1 cut(s)	1871
BceAI	ACGGC	1 cut(s)	1443
BciT130I	CCWGG	3 cut(s)	153, 1443, 1563
BciVI	GTATCC	1 cut(s)	2056
BclI	TGATCA	1 cut(s)	376
BcnI	CCSGG	2 cut(s)	1005, 2417
BcoDI	GTCTC	3 cut(s)	691, 2266, 2274
BcuI	ACTAGT	1 cut(s)	1772
BfaI	CTAG	7 cut(s)	257, 330, 690, 704, 1668, 1773, 2544
BfoI	RGCGCY	1 cut(s)	1795
BfuI	GTATCC	1 cut(s)	2056
BglI	GCCNNNNNGGC	1 cut(s)	1838
BglII	AGATCT	1 cut(s)	2185
BisI	GCNGC	1 cut(s)	1885
BlnI	CCTAGG	1 cut(s)	2543
BlsI	GCNGC	1 cut(s)	1886
Bme1390I	CCNGG	5 cut(s)	153, 1005, 1443, 1563, 2417
BmeT110I	CYCGRG	1 cut(s)	196
BmgT120I	GGNCC	2 cut(s)	952, 2355
BmiI	GGNNCC	5 cut(s)	222, 493, 649, 1665, 2643
BmrFI	CCNGG	5 cut(s)	153, 1005, 1443, 1563, 2417
BmrI	ACTGGG	1 cut(s)	509
BmsI	GCATC	9 cut(s)	302, 344, 797, 1083, 1252, 1477, 1525, 2147, 2374
BmtI	GCTAGC	1 cut(s)	260
BmuI	ACTGGG	1 cut(s)	509
BpiI	GAAGAC	1 cut(s)	48
Bpu10I	CCTNAGC	1 cut(s)	1392
BpuEI	CTTGAG	1 cut(s)	438
BpuMI	CCSGG	2 cut(s)	1005, 2417
BsaAI	YACGTR	1 cut(s)	1654
BsaBI	GATNNNNATC	1 cut(s)	2184
BsaI	GGTCTC	1 cut(s)	691
BsaJI	CCNNGG	9 cut(s)	152, 570, 1442, 1459, 1561, 1761, 1945, 2415, 2543
BsaXI	ACNNNNNCTCC	2 cut(s)	1108, 1138
Bsc4I	CCNNNNNNNGG	8 cut(s)	501, 1084, 1478, 1562, 1683, 1801, 1840, 2559
Bse1I	ACTGG	9 cut(s)	242, 299, 341, 504, 1112, 1295, 2020, 2067, 2478
Bse21I	CCTNAGG	1 cut(s)	1835
Bse3DI	GCAATG	2 cut(s)	1852, 2637
Bse8I	GATNNNNATC	1 cut(s)	2184
BseBI	CCWGG	3 cut(s)	153, 1443, 1563
BseDI	CCNNGG	9 cut(s)	152, 570, 1442, 1459, 1561, 1761, 1945, 2415, 2543
BseGI	GGATG	9 cut(s)	55, 535, 923, 1267, 1468, 1540, 1557, 1861, 2107
BseJI	GATNNNNATC	1 cut(s)	2184
BseLI	CCNNNNNNNGG	8 cut(s)	501, 1084, 1478, 1562, 1683, 1801, 1840, 2559
BseMI	GCAATG	2 cut(s)	1852, 2637
BseMII	CTCAG	5 cut(s)	123, 159, 714, 1383, 1849
BseNI	ACTGG	9 cut(s)	242, 299, 341, 504, 1112, 1295, 2020, 2067, 2478
BseRI	GAGGAG	5 cut(s)	871, 1151, 1370, 1388, 2600
BseSI	GKGCMC	1 cut(s)	2281
BseXI	GCAGC	1 cut(s)	1871
BseYI	CCCAGC	1 cut(s)	287
BsgI	GTGCAG	2 cut(s)	1202, 1870
BshFI	GGCC	8 cut(s)	595, 700, 745, 954, 1346, 1458, 2106, 2357
BshNI	GGYRCC	2 cut(s)	491, 1663
BsiHKAI	GWGCWC	3 cut(s)	174, 1331, 2281
BsiHKCI	CYCGRG	1 cut(s)	196
BsiSI	CCGG	2 cut(s)	1004, 2417
BslFI	GGGAC	1 cut(s)	1539
BslI	CCNNNNNNNGG	8 cut(s)	501, 1084, 1478, 1562, 1683, 1801, 1840, 2559
BsmAI	GTCTC	3 cut(s)	691, 2266, 2274
BsmFI	GGGAC	1 cut(s)	1539
BsmI	GAATGC	1 cut(s)	2083
BsnI	GGCC	8 cut(s)	595, 700, 745, 954, 1346, 1458, 2106, 2357
Bso31I	GGTCTC	1 cut(s)	691
BsoBI	CYCGRG	1 cut(s)	196
Bsp1286I	GDGCHC	4 cut(s)	174, 1010, 1331, 2281
Bsp143I	GATC	6 cut(s)	376, 1624, 2044, 2185, 2494, 2547
BspACI	CCGC	5 cut(s)	254, 388, 1064, 1802, 1841
BspANI	GGCC	8 cut(s)	595, 700, 745, 954, 1346, 1458, 2106, 2357
BspCNI	CTCAG	5 cut(s)	124, 160, 715, 1384, 1848
BspLI	GGNNCC	5 cut(s)	222, 493, 649, 1665, 2643
BspOI	GCTAGC	1 cut(s)	260
BspPI	GGATC	3 cut(s)	1619, 2502, 2555
BspT107I	GGYRCC	2 cut(s)	491, 1663
BspTNI	GGTCTC	1 cut(s)	691
BsrDI	GCAATG	2 cut(s)	1852, 2637
BsrI	ACTGG	9 cut(s)	242, 299, 341, 504, 1112, 1295, 2020, 2067, 2478
BssECI	CCNNGG	9 cut(s)	152, 570, 1442, 1459, 1561, 1761, 1945, 2415, 2543
BssMI	GATC	6 cut(s)	376, 1624, 2044, 2185, 2494, 2547
BssT1I	CCWWGG	4 cut(s)	570, 1761, 1945, 2543
Bst2UI	CCWGG	3 cut(s)	153, 1443, 1563
Bst6I	CTCTTC	2 cut(s)	736, 1329
BstAPI	GCANNNNNTGC	1 cut(s)	1228
BstBAI	YACGTR	1 cut(s)	1654
BstC8I	GCNNGC	6 cut(s)	258, 262, 786, 1843, 2217, 2567
BstDEI	CTNAG	8 cut(s)	132, 168, 723, 984, 1392, 1788, 1835, 2289
BstDSI	CCRYGG	1 cut(s)	1459
BstEII	GGTNACC	1 cut(s)	907
BstF5I	GGATG	9 cut(s)	55, 535, 923, 1267, 1468, 1540, 1557, 1861, 2107
BstH2I	RGCGCY	1 cut(s)	1795
BstHHI	GCGC	1 cut(s)	1794
BstKTI	GATC	6 cut(s)	379, 1627, 2047, 2188, 2497, 2550
BstMAI	GTCTC	3 cut(s)	691, 2266, 2274
BstMBI	GATC	6 cut(s)	376, 1624, 2044, 2185, 2494, 2547
BstMWI	GCNNNNNNNGC	3 cut(s)	1228, 1838, 2641
BstNI	CCWGG	3 cut(s)	153, 1443, 1563
BstNSI	RCATGY	1 cut(s)	2219
BstPI	GGTNACC	1 cut(s)	907
BstSCI	CCNGG	5 cut(s)	151, 1003, 1441, 1561, 2415
BstSLI	GKGCMC	1 cut(s)	2281
BstSNI	TACGTA	1 cut(s)	1654
BstV1I	GCAGC	1 cut(s)	1871
BstV2I	GAAGAC	1 cut(s)	48
BstX2I	RGATCY	2 cut(s)	2185, 2494
BstYI	RGATCY	2 cut(s)	2185, 2494
Bsu36I	CCTNAGG	1 cut(s)	1835
BsuI	GTATCC	1 cut(s)	2056
BsuRI	GGCC	8 cut(s)	595, 700, 745, 954, 1346, 1458, 2106, 2357
BtgI	CCRYGG	1 cut(s)	1459
BtsCI	GGATG	9 cut(s)	55, 535, 923, 1267, 1468, 1540, 1557, 1861, 2107
BtsI	GCAGTG	2 cut(s)	1164, 2044
BtsIMutI	CAGTG	5 cut(s)	1164, 2044, 2281, 2375, 2511
Cac8I	GCNNGC	6 cut(s)	258, 262, 786, 1843, 2217, 2567
CaiI	CAGNNNCTG	1 cut(s)	2658
CfoI	GCGC	1 cut(s)	1794
Cfr13I	GGNCC	2 cut(s)	952, 2355
Csp6I	GTAC	3 cut(s)	76, 2127, 2256
CspCI	CAANNNNNGTGG	2 cut(s)	2619, 2654
CviAII	CATG	6 cut(s)	1082, 1244, 1589, 1605, 2216, 2573
CviQI	GTAC	3 cut(s)	76, 2127, 2256
DdeI	CTNAG	8 cut(s)	132, 168, 723, 984, 1392, 1788, 1835, 2289
DpnI	GATC	6 cut(s)	378, 1626, 2046, 2187, 2496, 2549
DpnII	GATC	6 cut(s)	376, 1624, 2044, 2185, 2494, 2547
DraI	TTTAAA	2 cut(s)	1041, 1893
DrdI	GACNNNNNNGTC	1 cut(s)	1500
DseDI	GACNNNNNNGTC	1 cut(s)	1500
Eam1104I	CTCTTC	2 cut(s)	736, 1329
EarI	CTCTTC	2 cut(s)	736, 1329
Eco105I	TACGTA	1 cut(s)	1654
Eco130I	CCWWGG	4 cut(s)	570, 1761, 1945, 2543
Eco147I	AGGCCT	2 cut(s)	700, 1346
Eco24I	GRGCYC	1 cut(s)	1010
Eco31I	GGTCTC	1 cut(s)	691
Eco57I	CTGAAG	7 cut(s)	132, 492, 540, 717, 1411, 2300, 2635
Eco81I	CCTNAGG	1 cut(s)	1835
Eco88I	CYCGRG	1 cut(s)	196
Eco91I	GGTNACC	1 cut(s)	907
EcoO65I	GGTNACC	1 cut(s)	907
EcoRI	GAATTC	1 cut(s)	1643
EcoRII	CCWGG	3 cut(s)	151, 1441, 1561
EcoT14I	CCWWGG	4 cut(s)	570, 1761, 1945, 2543
EcoT22I	ATGCAT	1 cut(s)	2140
EcoT38I	GRGCYC	1 cut(s)	1010
ErhI	CCWWGG	4 cut(s)	570, 1761, 1945, 2543
FaeI	CATG	6 cut(s)	1085, 1247, 1592, 1608, 2219, 2576
FalI	AAGNNNNNCTT	2 cut(s)	1331, 1363
FaqI	GGGAC	1 cut(s)	1539
FatI	CATG	6 cut(s)	1081, 1243, 1588, 1604, 2215, 2572
FauI	CCCGC	2 cut(s)	1071, 1834
FbaI	TGATCA	1 cut(s)	376
FblI	GTMKAC	3 cut(s)	362, 410, 989
Fnu4HI	GCNGC	1 cut(s)	1885
FokI	GGATG	9 cut(s)	62, 542, 910, 1274, 1455, 1544, 1547, 1868, 2114
FriOI	GRGCYC	1 cut(s)	1010
Fsp4HI	GCNGC	1 cut(s)	1885
FspBI	CTAG	7 cut(s)	257, 330, 690, 704, 1668, 1773, 2544
GlaI	GCGC	1 cut(s)	1793
GluI	GCNGC	1 cut(s)	1885
GsaI	CCCAGC	1 cut(s)	291
HaeII	RGCGCY	1 cut(s)	1795
HaeIII	GGCC	8 cut(s)	595, 700, 745, 954, 1346, 1458, 2106, 2357
HapII	CCGG	2 cut(s)	1004, 2417
HhaI	GCGC	1 cut(s)	1794
Hin1II	CATG	6 cut(s)	1085, 1247, 1592, 1608, 2219, 2576
Hin6I	GCGC	1 cut(s)	1792
HinP1I	GCGC	1 cut(s)	1792
HincII	GTYRAC	3 cut(s)	363, 990, 2295
HindII	GTYRAC	3 cut(s)	363, 990, 2295
HindIII	AAGCTT	4 cut(s)	270, 315, 672, 960
HpaI	GTTAAC	1 cut(s)	2295
HpaII	CCGG	2 cut(s)	1004, 2417
HphI	GGTGA	8 cut(s)	228, 288, 330, 345, 1108, 1415, 1577, 2542
Hpy166II	GTNNAC	7 cut(s)	363, 411, 884, 990, 1999, 2279, 2295
Hpy188III	TCNNGA	9 cut(s)	704, 934, 946, 1127, 1479, 1547, 1618, 2537, 2587
Hpy8I	GTNNAC	7 cut(s)	363, 411, 884, 990, 1999, 2279, 2295
Hpy99I	CGWCG	2 cut(s)	364, 2524
HpyAV	CCTTC	7 cut(s)	525, 943, 1336, 1345, 1408, 1675, 1846
HpyCH4IV	ACGT	2 cut(s)	1653, 2519
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1228, 1838, 2641
HpyF3I	CTNAG	8 cut(s)	132, 168, 723, 984, 1392, 1788, 1835, 2289
HpySE526I	ACGT	2 cut(s)	1653, 2519
Hsp92II	CATG	6 cut(s)	1085, 1247, 1592, 1608, 2219, 2576
HspAI	GCGC	1 cut(s)	1792
Ksp22I	TGATCA	1 cut(s)	376
KspAI	GTTAAC	1 cut(s)	2295
Kzo9I	GATC	6 cut(s)	376, 1624, 2044, 2185, 2494, 2547
LmnI	GCTCC	2 cut(s)	2613, 2641
Lsp1109I	GCAGC	1 cut(s)	1871
LweI	GCATC	9 cut(s)	302, 344, 797, 1083, 1252, 1477, 1525, 2147, 2374
MaeI	CTAG	7 cut(s)	257, 330, 690, 704, 1668, 1773, 2544
MaeII	ACGT	2 cut(s)	1653, 2519
MaeIII	GTNAC	7 cut(s)	868, 907, 1114, 1513, 1600, 1691, 2143
MalI	GATC	6 cut(s)	378, 1626, 2046, 2187, 2496, 2549
MboI	GATC	6 cut(s)	376, 1624, 2044, 2185, 2494, 2547
MflI	RGATCY	2 cut(s)	2185, 2494
MhlI	GDGCHC	4 cut(s)	174, 1010, 1331, 2281
MlyI	GAGTC	1 cut(s)	1117
MmeI	TCCRAC	5 cut(s)	124, 1399, 1911, 2040, 2406
Mph1103I	ATGCAT	1 cut(s)	2140
MroXI	GAANNNNTTC	1 cut(s)	2594
MslI	CAYNNNNRTG	1 cut(s)	1050
MspA1I	CMGCKG	1 cut(s)	131
MspI	CCGG	2 cut(s)	1004, 2417
MspR9I	CCNGG	5 cut(s)	153, 1005, 1443, 1563, 2417
Mva1269I	GAATGC	1 cut(s)	2083
MvaI	CCWGG	3 cut(s)	153, 1443, 1563
MwoI	GCNNNNNNNGC	3 cut(s)	1228, 1838, 2641
NciI	CCSGG	2 cut(s)	1005, 2417
NdeII	GATC	6 cut(s)	376, 1624, 2044, 2185, 2494, 2547
NheI	GCTAGC	1 cut(s)	256
NlaIII	CATG	6 cut(s)	1085, 1247, 1592, 1608, 2219, 2576
NlaIV	GGNNCC	5 cut(s)	222, 493, 649, 1665, 2643
NmuCI	GTSAC	3 cut(s)	1114, 1691, 2143
NsiI	ATGCAT	1 cut(s)	2140
NspI	RCATGY	1 cut(s)	2219
OliI	CACNNNNGTG	1 cut(s)	1050
PaeI	GCATGC	1 cut(s)	2219
PaeR7I	CTCGAG	1 cut(s)	196
PceI	AGGCCT	2 cut(s)	700, 1346
PctI	GAATGC	1 cut(s)	2083
PdmI	GAANNNNTTC	1 cut(s)	2594
PfeI	GAWTC	9 cut(s)	97, 467, 524, 602, 758, 1498, 1955, 1990, 2655
PkrI	GCNGC	1 cut(s)	1886
PleI	GAGTC	1 cut(s)	1117
PpsI	GAGTC	1 cut(s)	1117
Ppu21I	YACGTR	1 cut(s)	1654
PshBI	ATTAAT	3 cut(s)	432, 1521, 1632
Psp6I	CCWGG	3 cut(s)	151, 1441, 1561
PspEI	GGTNACC	1 cut(s)	907
PspFI	CCCAGC	1 cut(s)	287
PspGI	CCWGG	3 cut(s)	151, 1441, 1561
PspN4I	GGNNCC	5 cut(s)	222, 493, 649, 1665, 2643
PspPI	GGNCC	2 cut(s)	952, 2355
PspXI	VCTCGAGB	1 cut(s)	196
PstNI	CAGNNNCTG	1 cut(s)	2658
PsuI	RGATCY	2 cut(s)	2185, 2494
PvuII	CAGCTG	1 cut(s)	131
RsaI	GTAC	3 cut(s)	77, 2128, 2257
RsaNI	GTAC	3 cut(s)	76, 2127, 2256
RseI	CAYNNNNRTG	1 cut(s)	1050
SalI	GTCGAC	2 cut(s)	361, 988
SatI	GCNGC	1 cut(s)	1885
Sau3AI	GATC	6 cut(s)	376, 1624, 2044, 2185, 2494, 2547
Sau96I	GGNCC	2 cut(s)	952, 2355
SchI	GAGTC	1 cut(s)	1117
ScrFI	CCNGG	5 cut(s)	153, 1005, 1443, 1563, 2417
SduI	GDGCHC	4 cut(s)	174, 1010, 1331, 2281
SfaNI	GCATC	9 cut(s)	302, 344, 797, 1083, 1252, 1477, 1525, 2147, 2374
Sfr274I	CTCGAG	1 cut(s)	196
SlaI	CTCGAG	1 cut(s)	196
SmiMI	CAYNNNNRTG	1 cut(s)	1050
SmlI	CTYRAG	2 cut(s)	196, 453
SmoI	CTYRAG	2 cut(s)	196, 453
SnaBI	TACGTA	1 cut(s)	1654
SpeI	ACTAGT	1 cut(s)	1772
SphI	GCATGC	1 cut(s)	2219
SseBI	AGGCCT	2 cut(s)	700, 1346
SsiI	CCGC	5 cut(s)	254, 388, 1064, 1802, 1841
SspMI	CTAG	7 cut(s)	257, 330, 690, 704, 1668, 1773, 2544
StuI	AGGCCT	2 cut(s)	700, 1346
StyD4I	CCNGG	5 cut(s)	151, 1003, 1441, 1561, 2415
StyI	CCWWGG	4 cut(s)	570, 1761, 1945, 2543
TaiI	ACGT	2 cut(s)	1656, 2522
TaqI	TCGA	6 cut(s)	197, 362, 989, 1453, 2522, 2586
TfiI	GAWTC	9 cut(s)	97, 467, 524, 602, 758, 1498, 1955, 1990, 2655
TscAI	CASTG	5 cut(s)	1164, 2044, 2281, 2382, 2511
TseFI	GTSAC	3 cut(s)	1114, 1691, 2143
TseI	GCWGC	1 cut(s)	1884
Tsp45I	GTSAC	3 cut(s)	1114, 1691, 2143
TspGWI	ACGGA	4 cut(s)	1346, 1510, 1742, 2312
TspRI	CASTG	5 cut(s)	1164, 2044, 2281, 2382, 2511
VneI	GTGCAC	1 cut(s)	2277
VspI	ATTAAT	3 cut(s)	432, 1521, 1632
XapI	RAATTY	5 cut(s)	31, 307, 968, 1643, 2407
XbaI	TCTAGA	1 cut(s)	703
XceI	RCATGY	1 cut(s)	2219
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1287
XhoI	CTCGAG	1 cut(s)	196
XmaJI	CCTAGG	1 cut(s)	2543
XmiI	GTMKAC	3 cut(s)	362, 410, 989
XmnI	GAANNNNTTC	1 cut(s)	2594
XspI	CTAG	7 cut(s)	257, 330, 690, 704, 1668, 1773, 2544
Zsp2I	ATGCAT	1 cut(s)	2140

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.