Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0005383

Physical Location & Seq

Genomic Coordinates Reverse (-)

20968461 .. 20982746

Gene Structure 14286 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000007780

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1524 bp

ATGTATGCGAAGATGAGAGACTCAGATTATGTGAAGAAACAAACATTCAGAGACAACTTCTGGAACGATTGGTGTAAGCTGCTTGGCAATGACCATGTAATTCAGGACTTGGGTGGTTGTGATTTCACTCCAATATATGATAGGTATCGAAATGAATTGCAGAAGAAGAAACAGAGGAGTGACAAAGAGAAGAAAGCCGTCAAGCAAGAGGACTGGAAACAACAGCTGAAGTATAAGTGGGCTCTCGTTGATGGTATCAGAAAAGAGCAAACTCGGGTTAGACCACCTGGGTTATTCCGAGGACGTGGGGATCGAGCACCCAAAGTCAGGAAAGCTTGTAAGGCGGATTTCTCCAAAAGATGTTCAATAAATACAGGAAAGGGCGCCCTGATTCCAGAATGTCCAATCCCTGGTGAAAGCTGGAAAGAAATAAGGCATGAGAATACTGTGACCTGGTTAGCTTTCTACCAACACCCAATTAATCAAAAGGAACTCACATACATGCATGTTTCTAGCAGGAACTCCGCACTTAGTACTTCCTTGAAGAGGCAAAGTGACAAGGAGAAATTTGAGAAAGCAAGGAAGTTGAAGGGGTTTTTATTGAAATATGACTACATAGGAGCTATCGGGGCTGCATACACTTGGGATATTACAAGTGAGGATGGTCAAAAGCGGCAGCTAGCAGTGGCTACCTATCTCATGGATAAACTTGGTCTGAGGGCTGGAATTGAGAAGGATGATGATGAAGCTGATATTGTCAGTTGCCGGAGATTAAAAGTAGAGAATGTAAAAGCAATATCCCCTATCTTCTTCCTCGGTAAAGATTTAATCAGATATAAAAATGCTGTTGAAGTTGATCTTCCTGTTTGTGAGGCAATTGTAATATTTCAGGCCGGTAAGTGTAGCAGTGACAAACTGTTTGACAAGTTGGACACCCGTAAATTGAATGCACATTTAAAGGCACTGATGTCCGGCCTTACAGCGGAAGTATTGCGTGCAGCCTTGAGCAAGGGGACTATAGAGGGAGATGTTTCAGAGAAACTTGATGTTTATGCCCGTGCCAAGGAGGTTGCTATCACTTGTAATCATCTGTGCAGTACCTCAAAGACTGATAGTGAACAAACCTCTAGGTTAATGCTGAAGATTTACGAGCTCCTGTCCCCAGCGAGAGATTCTTATGGAAAACGAAAGAGTGATTCCGCTCGAGAAGATCAGAATGAGGACATTGAAATTGTGAATAAATTGCCAACAGCCACAATGATTGATCCTAGAATCACTGCTGCATGGTGCAGGAGACATGGAGTTCCCATTGACAGGGTTGGTCTGGCCTATTGCTCTTTCCTTGTGTCTATTTTGCTTTACGGGGTTCATTTACAGGTTCAGCAGGTCAAAGACATAGACAAGCTGGGTTTTATGGGTCTGGTTCAAGAAGAGCTTGTGTTGATAGAAGACTGGTACATTTTCAGACAAGACCGTTGGGGATTGGTGGAGGGTTGTCACTTGAGACAAGAAAACCAATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

508

Amino Acids

58.22

Weight (kDa)

9.1

Isoelectric Point (pI)

42.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Topoisom_I_N	PF02919	1 - 163	2.4e-42	Eukaryotic DNA topoisomerase I, DNA binding fragment
Topoisom_I	PF01028	179 - 384	2.7e-46	Eukaryotic DNA topoisomerase I, catalytic core

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005383)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G55310
fragaria_vesca	FvH4_4g16730 FvH4_4g16730
malus_domestica	MD00G1109500.v1.1 MD13G1176900.v1.1 MD16G1178600.v1.1
prunus_persica	Prupe.1G146000_v2.0.a1 Prupe.1G146000_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1
pyrus_communis	pycom10g21080 pycom13g15260 pycom16g15010
rosa_chinensis	RchiOBHm_Chr4g0420301
rosa_laevigata	RLG00000003998 RLG00000007777 RLG00000007780
rosa_multiflora	Rmu_sc0001767.1_g000005
rosa_roxburghii	Rroxscaffold_5G00362940
rosa_rugosa	Rorug04G0163000 Rorug04G0163100 Rorug04G0163200
rosa_samantha	Rh4AG224100 Rh4BG227100 Rh4CG238200
rosa_wichuraiana	Rw4G019300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1375
AccB1I	GGYRCC	1 cut(s)	383
AccB7I	CCANNNNNTGG	1 cut(s)	410
AccBSI	CCGCTC	1 cut(s)	1202
AciI	CCGC	5 cut(s)	344, 525, 673, 983, 1200
AclWI	GGATC	2 cut(s)	318, 1259
AcsI	RAATTY	1 cut(s)	566
AcuI	CTGAAG	2 cut(s)	248, 1160
AcyI	GRCGYC	1 cut(s)	384
AfaI	GTAC	3 cut(s)	535, 1099, 1457
AfiI	CCNNNNNNNGG	6 cut(s)	327, 410, 546, 982, 1063, 1314
AgsI	TTSAA	8 cut(s)	366, 544, 589, 604, 851, 946, 1229, 1427
AjiI	CACGTC	1 cut(s)	305
AjnI	CCWGG	3 cut(s)	286, 409, 452
Alw21I	GWGCWC	2 cut(s)	319, 1155
Alw26I	GTCTC	4 cut(s)	12, 45, 1288, 1498
AlwI	GGATC	2 cut(s)	318, 1259
Ama87I	CYCGRG	2 cut(s)	273, 1203
AoxI	GGCC	3 cut(s)	891, 973, 1326
ApeKI	GCWGC	5 cut(s)	79, 632, 676, 998, 1280
ApoI	RAATTY	1 cut(s)	566
ArsI	GACNNNNNNTTYG	2 cut(s)	902, 934
AseI	ATTAAT	1 cut(s)	480
AspLEI	GCGC	1 cut(s)	386
AsuHPI	GGTGA	1 cut(s)	425
AsuNHI	GCTAGC	1 cut(s)	679
AvaI	CYCGRG	2 cut(s)	273, 1203
BanI	GGYRCC	1 cut(s)	383
BanII	GRGCYC	2 cut(s)	244, 1155
BbsI	GAAGAC	1 cut(s)	1455
Bbv12I	GWGCWC	2 cut(s)	319, 1155
BbvI	GCAGC	5 cut(s)	66, 619, 688, 1010, 1267
BccI	CCATC	2 cut(s)	245, 656
BceAI	ACGGC	1 cut(s)	182
BciT130I	CCWGG	3 cut(s)	288, 411, 454
BcoDI	GTCTC	4 cut(s)	12, 45, 1288, 1498
BfaI	CTAG	4 cut(s)	513, 680, 1128, 1269
BfmI	CTRYAG	1 cut(s)	1017
BfoI	RGCGCY	1 cut(s)	387
BfuAI	ACCTGC	1 cut(s)	1375
BisI	GCNGC	6 cut(s)	80, 633, 674, 677, 999, 1281
BlsI	GCNGC	6 cut(s)	81, 634, 675, 678, 1000, 1282
BmcAI	AGTACT	1 cut(s)	535
Bme1390I	CCNGG	3 cut(s)	288, 411, 454
BmeT110I	CYCGRG	2 cut(s)	273, 1203
BmgBI	CACGTC	1 cut(s)	305
BmiI	GGNNCC	1 cut(s)	385
BmrFI	CCNGG	3 cut(s)	288, 411, 454
BmtI	GCTAGC	1 cut(s)	683
BpiI	GAAGAC	1 cut(s)	1455
BpuEI	CTTGAG	2 cut(s)	1024, 1522
BsaBI	GATNNNNATC	1 cut(s)	144
BsaHI	GRCGYC	1 cut(s)	384
BsaJI	CCNNGG	5 cut(s)	287, 298, 409, 814, 1062
Bsc4I	CCNNNNNNNGG	6 cut(s)	327, 410, 546, 982, 1063, 1314
Bse118I	RCCGGY	1 cut(s)	893
Bse1I	ACTGG	2 cut(s)	218, 1457
Bse3DI	GCAATG	1 cut(s)	94
Bse8I	GATNNNNATC	1 cut(s)	144
BseBI	CCWGG	3 cut(s)	288, 411, 454
BseDI	CCNNGG	5 cut(s)	287, 298, 409, 814, 1062
BseGI	GGATG	2 cut(s)	667, 742
BseJI	GATNNNNATC	1 cut(s)	144
BseLI	CCNNNNNNNGG	6 cut(s)	327, 410, 546, 982, 1063, 1314
BseMI	GCAATG	1 cut(s)	94
BseMII	CTCAG	2 cut(s)	36, 707
BseNI	ACTGG	2 cut(s)	218, 1457
BseRI	GAGGAG	1 cut(s)	190
BseXI	GCAGC	5 cut(s)	66, 619, 688, 1010, 1267
BseYI	CCCAGC	2 cut(s)	1162, 1405
BsgI	GTGCAG	3 cut(s)	1017, 1114, 1309
BshFI	GGCC	3 cut(s)	893, 975, 1328
BshNI	GGYRCC	1 cut(s)	383
BsiHKAI	GWGCWC	2 cut(s)	319, 1155
BsiHKCI	CYCGRG	2 cut(s)	273, 1203
BsiSI	CCGG	3 cut(s)	766, 894, 972
BslFI	GGGAC	2 cut(s)	1027, 1144
BslI	CCNNNNNNNGG	6 cut(s)	327, 410, 546, 982, 1063, 1314
BsmAI	GTCTC	4 cut(s)	12, 45, 1288, 1498
BsmFI	GGGAC	2 cut(s)	1027, 1144
BsmI	GAATGC	1 cut(s)	952
BsnI	GGCC	3 cut(s)	893, 975, 1328
BsoBI	CYCGRG	2 cut(s)	273, 1203
Bsp1286I	GDGCHC	3 cut(s)	244, 319, 1155
Bsp143I	GATC	4 cut(s)	310, 856, 1210, 1264
BspACI	CCGC	5 cut(s)	344, 525, 673, 983, 1200
BspANI	GGCC	3 cut(s)	893, 975, 1328
BspCNI	CTCAG	2 cut(s)	35, 708
BspHI	TCATGA	1 cut(s)	1520
BspLI	GGNNCC	1 cut(s)	385
BspMI	ACCTGC	1 cut(s)	1375
BspOI	GCTAGC	1 cut(s)	683
BspPI	GGATC	2 cut(s)	318, 1259
BspQI	GCTCTTC	1 cut(s)	1425
BspT107I	GGYRCC	1 cut(s)	383
BsrBI	CCGCTC	1 cut(s)	1202
BsrDI	GCAATG	1 cut(s)	94
BsrFI	RCCGGY	1 cut(s)	893
BsrI	ACTGG	2 cut(s)	218, 1457
BssAI	RCCGGY	1 cut(s)	893
BssECI	CCNNGG	5 cut(s)	287, 298, 409, 814, 1062
BssMI	GATC	4 cut(s)	310, 856, 1210, 1264
BssNI	GRCGYC	1 cut(s)	384
BssT1I	CCWWGG	1 cut(s)	1062
Bst2UI	CCWGG	3 cut(s)	288, 411, 454
Bst4CI	ACNGT	3 cut(s)	448, 918, 1475
Bst6I	CTCTTC	2 cut(s)	539, 1425
BstACI	GRCGYC	1 cut(s)	384
BstC8I	GCNNGC	2 cut(s)	681, 996
BstDEI	CTNAG	3 cut(s)	22, 530, 716
BstENI	CCTNNNNNAGG	1 cut(s)	544
BstF5I	GGATG	2 cut(s)	667, 742
BstH2I	RGCGCY	1 cut(s)	387
BstHHI	GCGC	1 cut(s)	386
BstKTI	GATC	4 cut(s)	313, 859, 1213, 1267
BstMAI	GTCTC	4 cut(s)	12, 45, 1288, 1498
BstMBI	GATC	4 cut(s)	310, 856, 1210, 1264
BstMWI	GCNNNNNNNGC	2 cut(s)	341, 629
BstNI	CCWGG	3 cut(s)	288, 411, 454
BstNSI	RCATGY	2 cut(s)	505, 509
BstSCI	CCNGG	3 cut(s)	286, 409, 452
BstSFI	CTRYAG	1 cut(s)	1017
BstV1I	GCAGC	5 cut(s)	66, 619, 688, 1010, 1267
BstV2I	GAAGAC	1 cut(s)	1455
BsuRI	GGCC	3 cut(s)	893, 975, 1328
BtrI	CACGTC	1 cut(s)	305
BtsCI	GGATG	2 cut(s)	667, 742
BtsI	GCAGTG	3 cut(s)	690, 913, 1275
BtsIMutI	CAGTG	4 cut(s)	690, 913, 962, 1275
BveI	ACCTGC	1 cut(s)	1375
Cac8I	GCNNGC	2 cut(s)	681, 996
CciI	TCATGA	1 cut(s)	1520
CfoI	GCGC	1 cut(s)	386
Cfr10I	RCCGGY	1 cut(s)	893
CsiI	ACCWGGT	1 cut(s)	452
Csp6I	GTAC	3 cut(s)	534, 1098, 1456
CspCI	CAANNNNNGTGG	2 cut(s)	1243, 1278
CviAII	CATG	8 cut(s)	95, 437, 502, 506, 700, 1284, 1298, 1521
CviQI	GTAC	3 cut(s)	534, 1098, 1456
DdeI	CTNAG	3 cut(s)	22, 530, 716
DinI	GGCGCC	1 cut(s)	385
DpnI	GATC	4 cut(s)	312, 858, 1212, 1266
DpnII	GATC	4 cut(s)	310, 856, 1210, 1264
DraI	TTTAAA	1 cut(s)	957
Eam1104I	CTCTTC	2 cut(s)	539, 1425
EarI	CTCTTC	2 cut(s)	539, 1425
EciI	GGCGGA	1 cut(s)	359
Ecl136II	GAGCTC	1 cut(s)	1153
Eco130I	CCWWGG	1 cut(s)	1062
Eco24I	GRGCYC	2 cut(s)	244, 1155
Eco53kI	GAGCTC	1 cut(s)	1153
Eco57I	CTGAAG	2 cut(s)	248, 1160
Eco88I	CYCGRG	2 cut(s)	273, 1203
EcoICRI	GAGCTC	1 cut(s)	1153
EcoNI	CCTNNNNNAGG	1 cut(s)	544
EcoRII	CCWGG	3 cut(s)	286, 409, 452
EcoT14I	CCWWGG	1 cut(s)	1062
EcoT22I	ATGCAT	1 cut(s)	507
EcoT38I	GRGCYC	2 cut(s)	244, 1155
EgeI	GGCGCC	1 cut(s)	385
EheI	GGCGCC	1 cut(s)	385
ErhI	CCWWGG	1 cut(s)	1062
FaeI	CATG	8 cut(s)	98, 440, 505, 509, 703, 1287, 1301, 1524
FalI	AAGNNNNNCTT	4 cut(s)	843, 875, 1419, 1451
FaqI	GGGAC	2 cut(s)	1027, 1144
FatI	CATG	8 cut(s)	94, 436, 501, 505, 699, 1283, 1297, 1520
Fnu4HI	GCNGC	6 cut(s)	80, 633, 674, 677, 999, 1281
FokI	GGATG	2 cut(s)	674, 749
FriOI	GRGCYC	2 cut(s)	244, 1155
Fsp4HI	GCNGC	6 cut(s)	80, 633, 674, 677, 999, 1281
FspBI	CTAG	4 cut(s)	513, 680, 1128, 1269
GlaI	GCGC	1 cut(s)	385
GluI	GCNGC	6 cut(s)	80, 633, 674, 677, 999, 1281
GsaI	CCCAGC	2 cut(s)	1166, 1409
HaeII	RGCGCY	1 cut(s)	387
HaeIII	GGCC	3 cut(s)	893, 975, 1328
HapII	CCGG	3 cut(s)	766, 894, 972
HhaI	GCGC	1 cut(s)	386
Hin1I	GRCGYC	1 cut(s)	384
Hin1II	CATG	8 cut(s)	98, 440, 505, 509, 703, 1287, 1301, 1524
Hin6I	GCGC	1 cut(s)	384
HinP1I	GCGC	1 cut(s)	384
HindIII	AAGCTT	1 cut(s)	333
HinfI	GANTC	5 cut(s)	20, 391, 1172, 1196, 1272
HpaII	CCGG	3 cut(s)	766, 894, 972
HphI	GGTGA	1 cut(s)	425
Hpy166II	GTNNAC	1 cut(s)	1118
Hpy188I	TCNGA	9 cut(s)	25, 50, 260, 299, 717, 833, 1036, 1215, 1466
Hpy188III	TCNNGA	7 cut(s)	61, 104, 328, 395, 1205, 1427, 1521
Hpy8I	GTNNAC	1 cut(s)	1118
HpyAV	CCTTC	2 cut(s)	583, 727
HpyCH4III	ACNGT	3 cut(s)	448, 918, 1475
HpyCH4IV	ACGT	1 cut(s)	304
HpyCH4V	TGCA	8 cut(s)	160, 505, 635, 950, 998, 1095, 1283, 1290
HpyF10VI	GCNNNNNNNGC	2 cut(s)	341, 629
HpyF3I	CTNAG	3 cut(s)	22, 530, 716
HpySE526I	ACGT	1 cut(s)	304
Hsp92I	GRCGYC	1 cut(s)	384
Hsp92II	CATG	8 cut(s)	98, 440, 505, 509, 703, 1287, 1301, 1524
HspAI	GCGC	1 cut(s)	384
KasI	GGCGCC	1 cut(s)	383
Kzo9I	GATC	4 cut(s)	310, 856, 1210, 1264
LguI	GCTCTTC	1 cut(s)	1425
LmnI	GCTCC	2 cut(s)	620, 1158
Lsp1109I	GCAGC	5 cut(s)	66, 619, 688, 1010, 1267
MabI	ACCWGGT	1 cut(s)	452
MaeI	CTAG	4 cut(s)	513, 680, 1128, 1269
MaeII	ACGT	1 cut(s)	304
MaeIII	GTNAC	5 cut(s)	179, 448, 554, 908, 1496
MalI	GATC	4 cut(s)	312, 858, 1212, 1266
MbiI	CCGCTC	1 cut(s)	1202
MboI	GATC	4 cut(s)	310, 856, 1210, 1264
MfeI	CAATTG	1 cut(s)	876
MhlI	GDGCHC	3 cut(s)	244, 319, 1155
MluCI	AATT	9 cut(s)	99, 155, 477, 566, 726, 876, 941, 1230, 1241
Mly113I	GGCGCC	1 cut(s)	384
MlyI	GAGTC	1 cut(s)	14
MmeI	TCCRAC	1 cut(s)	909
Mph1103I	ATGCAT	1 cut(s)	507
MseI	TTAA	5 cut(s)	480, 773, 827, 956, 1133
MslI	CAYNNNNRTG	1 cut(s)	500
MspA1I	CMGCKG	2 cut(s)	226, 983
MspI	CCGG	3 cut(s)	766, 894, 972
MspR9I	CCNGG	3 cut(s)	288, 411, 454
MunI	CAATTG	1 cut(s)	876
Mva1269I	GAATGC	1 cut(s)	952
MvaI	CCWGG	3 cut(s)	288, 411, 454
MwoI	GCNNNNNNNGC	2 cut(s)	341, 629
NarI	GGCGCC	1 cut(s)	384
NdeII	GATC	4 cut(s)	310, 856, 1210, 1264
NheI	GCTAGC	1 cut(s)	679
NlaIII	CATG	8 cut(s)	98, 440, 505, 509, 703, 1287, 1301, 1524
NlaIV	GGNNCC	1 cut(s)	385
NmuCI	GTSAC	5 cut(s)	179, 448, 554, 908, 1496
NsiI	ATGCAT	1 cut(s)	507
NspI	RCATGY	2 cut(s)	505, 509
PaeR7I	CTCGAG	1 cut(s)	1203
PagI	TCATGA	1 cut(s)	1520
PciSI	GCTCTTC	1 cut(s)	1425
PcsI	WCGNNNNNNNCGW	1 cut(s)	310
PctI	GAATGC	1 cut(s)	952
PfeI	GAWTC	4 cut(s)	391, 1172, 1196, 1272
PflMI	CCANNNNNTGG	1 cut(s)	410
PkrI	GCNGC	6 cut(s)	81, 634, 675, 678, 1000, 1282
PleI	GAGTC	1 cut(s)	14
PluTI	GGCGCC	1 cut(s)	387
PpsI	GAGTC	1 cut(s)	14
PshBI	ATTAAT	1 cut(s)	480
Psp124BI	GAGCTC	1 cut(s)	1155
Psp6I	CCWGG	3 cut(s)	286, 409, 452
PspFI	CCCAGC	2 cut(s)	1162, 1405
PspGI	CCWGG	3 cut(s)	286, 409, 452
PspN4I	GGNNCC	1 cut(s)	385
PvuII	CAGCTG	1 cut(s)	226
RsaI	GTAC	3 cut(s)	535, 1099, 1457
RsaNI	GTAC	3 cut(s)	534, 1098, 1456
RseI	CAYNNNNRTG	1 cut(s)	500
SacI	GAGCTC	1 cut(s)	1155
SapI	GCTCTTC	1 cut(s)	1425
SaqAI	TTAA	5 cut(s)	480, 773, 827, 956, 1133
SatI	GCNGC	6 cut(s)	80, 633, 674, 677, 999, 1281
Sau3AI	GATC	4 cut(s)	310, 856, 1210, 1264
ScaI	AGTACT	1 cut(s)	535
SchI	GAGTC	1 cut(s)	14
ScrFI	CCNGG	3 cut(s)	288, 411, 454
SduI	GDGCHC	3 cut(s)	244, 319, 1155
SexAI	ACCWGGT	1 cut(s)	452
SfcI	CTRYAG	1 cut(s)	1017
SfoI	GGCGCC	1 cut(s)	385
Sfr274I	CTCGAG	1 cut(s)	1203
SlaI	CTCGAG	1 cut(s)	1203
SmiMI	CAYNNNNRTG	1 cut(s)	500
SmlI	CTYRAG	3 cut(s)	1003, 1203, 1501
SmoI	CTYRAG	3 cut(s)	1003, 1203, 1501
Sse9I	AATT	9 cut(s)	99, 155, 477, 566, 726, 876, 941, 1230, 1241
SsiI	CCGC	5 cut(s)	344, 525, 673, 983, 1200
SspDI	GGCGCC	1 cut(s)	383
SspI	AATATT	1 cut(s)	885
SspMI	CTAG	4 cut(s)	513, 680, 1128, 1269
SstI	GAGCTC	1 cut(s)	1155
StyD4I	CCNGG	3 cut(s)	286, 409, 452
StyI	CCWWGG	1 cut(s)	1062
TaaI	ACNGT	3 cut(s)	448, 918, 1475
TaiI	ACGT	1 cut(s)	307
TaqI	TCGA	3 cut(s)	148, 313, 1204
TasI	AATT	9 cut(s)	99, 155, 477, 566, 726, 876, 941, 1230, 1241
TatI	WGTACW	1 cut(s)	533
TauI	GCSGC	1 cut(s)	676
TfiI	GAWTC	4 cut(s)	391, 1172, 1196, 1272
Tru1I	TTAA	5 cut(s)	480, 773, 827, 956, 1133
Tru9I	TTAA	5 cut(s)	480, 773, 827, 956, 1133
TscAI	CASTG	4 cut(s)	690, 913, 969, 1282
TseFI	GTSAC	5 cut(s)	179, 448, 554, 908, 1496
TseI	GCWGC	5 cut(s)	79, 632, 676, 998, 1280
Tsp45I	GTSAC	5 cut(s)	179, 448, 554, 908, 1496
TspDTI	ATGAA	3 cut(s)	168, 759, 1358
TspRI	CASTG	4 cut(s)	690, 913, 969, 1282
Van91I	CCANNNNNTGG	1 cut(s)	410
VspI	ATTAAT	1 cut(s)	480
XagI	CCTNNNNNAGG	1 cut(s)	544
XapI	RAATTY	1 cut(s)	566
XceI	RCATGY	2 cut(s)	505, 509
XhoI	CTCGAG	1 cut(s)	1203
XspI	CTAG	4 cut(s)	513, 680, 1128, 1269
ZrmI	AGTACT	1 cut(s)	535
Zsp2I	ATGCAT	1 cut(s)	507

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.