Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0005383

Physical Location & Seq

Genomic Coordinates Reverse (-)

23820966 .. 23822885

Gene Structure 1920 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g21080.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1920 bp

ATGACAGCCAATGTACCATACATACACCCATGCTTTCTTCCAATAAGACCCCTAGATCATAGGGCAAATTCTTCACCTAAAAGGCCAAAAGTTTCACATGCTTGTTCTTCAACATCTACCCTAAAAATTAAGGAGGAAAAAGATGACGACTTGATTCAAATTTCCCAGAGGATCAAGGAACCTACGTTGTTGACAGAGAAGAAGGCAATGAGAGTCTGTTCATCCTCCACCAGGAAACCGATCAAAAAGGGCCAAAATGGTTTGAAACTTTCGAGTTGCCCTAAGTCGACGAATGTGCTACCGAGCTCTAATGATGAGCAGAAAAAATGGAAAACTTTGGTTCACAACGGTGTGATCTTTCCGCCTCCGTACGAGCCCCACGGTGTAAAGATGATGTATGAAGGGAAGCCGGTAGACTTGAGTCCGGAACAAGAGGAGGTCGCAACTATATACGCCGTGATGAAAGATACAGATTACGTGCAGAAGAACCACGTAATTCGGAGGTTGGATGGTTGCGATTTCACCCCAATATACGAGTGGTGTATGAAAGAAAAGCAAGACAGGAAACAAATGAGTAGGGAAGAGAAGAAGGCCGCGGGGGCGGAGAAAAAGAAACAAGAGGAGAAGTATGCGTGGGCGTTCGTTGATGGCGAAAAAGAGAGAGTTGGAAGCTTCAAAGTTGAACCTCCTGGATTGTTCAGAGGCCGAGGGGAGCATCCAAAATCGGGAAAGGTAAAAAGGCGGATTCTTCCAAGTGATGTTACGATCAACATGGGGCAGGATGCACCGGTTCCAGAATGTCCTGTCCCTGGCGAAAGGTGGAAGGAAGTGAGGCATGATGATACGGTTACATGGTTAGCTCTCTGGACAGATCCAATCAATCCAAAGAAATTCAAGTACGTGTCTCTTGGGGAGAGTAGCTCCTTGAAGATAGAAAGTGACCAGAAGAAGTACGAGAATGCGAGGAGGATGCTCAAGGACTACATTGGGAAAATCAGGGCAGATTACTCTAGGGATTTTACTAGTAAAGATTGCGCGAAGAGGCAGATAGCGGTTGCAACCTATCTCATCGATAAGCTTGCTCTGAGGGCGGGAAATGAAAAGGGAGAAGAGGAAGCTGATACTGTTGGTTGCTGCACATTGAAGGTAGAACATGTGAAAGCAATCGCCCCTAACTTGTTGGAGTTTAACTTCCTTGGTAAAGATTCGATCAGATACCAAAATACCGTTGAAGTTGAGGTTCCTGTTTACAAGGAAATTAGACAGTTCCAGGCCGGGAAACGCGGTGGTGATCATCTCTTCAACAAGCTGGATGCGAGTAGATTGAATGCTCATTTAAAGCAACAGATGCCTGGCCTTACGGCAAAAGTGCTTCGTACCTACAATGCTTTTATCACATTGGATGACTTGTTGAACAAGGAAATCAAAGACGAAGCAGATCTTTCAGAGAAAGTCGCTATTTATAACCACGCGAACAAAGAGGTTGCCATCATCTGTAATCACCAGCGTACAATTTCAAAGTCTCACGAAGAACAAATGTCTAAATTGATGGAAAAGATTGGCGGCCTTCTTGACAACCTCAAAGAGCTGAAAACAAAGTTGGAAAGGGCGAAAAGAGGAAAGCCACAGCCGCTGACTGATGCTGATGGAAAGAAGCGAAAGAGGAATTTGACCCCAGATGAGTTGGAGAAGAAGATAGCTCAAAAAACTGCGGACATCAGAAACATGGAGATGGGCATGAGAACGAAAGAGGATCTGAAAACAGTGGCGCTGGGAACATCGAAACTCAACTATCTTGACCCTAGGATCACGGTTGCATGGTGCAAGCGAAATCAAGTTCCAATTGAGAGGATGTTCAACACCACTCAACTGAGAAAGTTTGCTTGGGCAATGACCGTGGATCCTGACTTCAGATTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

640

Amino Acids

73.17

Weight (kDa)

9.38

Isoelectric Point (pI)

39.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Topoisom_I_N	PF02919	108 - 303	3.9e-75	Eukaryotic DNA topoisomerase I, DNA binding fragment
Topoisom_I	PF01028	306 - 533	2e-88	Eukaryotic DNA topoisomerase I, catalytic core
Topo_C_assoc	PF14370	573 - 639	9.4e-32	C-terminal topoisomerase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005383)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G55310
fragaria_vesca	FvH4_4g16730 FvH4_4g16730
malus_domestica	MD00G1109500.v1.1 MD13G1176900.v1.1 MD16G1178600.v1.1
prunus_persica	Prupe.1G146000_v2.0.a1 Prupe.1G146000_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1
pyrus_communis	pycom10g21080 pycom13g15260 pycom16g15010
rosa_chinensis	RchiOBHm_Chr4g0420301
rosa_laevigata	RLG00000003998 RLG00000007777 RLG00000007780
rosa_multiflora	Rmu_sc0001767.1_g000005
rosa_roxburghii	Rroxscaffold_5G00362940
rosa_rugosa	Rorug04G0163000 Rorug04G0163100 Rorug04G0163200
rosa_samantha	Rh4AG224100 Rh4BG227100 Rh4CG238200
rosa_wichuraiana	Rw4G019300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1464
AccI	GTMKAC	2 cut(s)	287, 414
AccII	CGCG	4 cut(s)	596, 1037, 1284, 1472
AccIII	TCCGGA	1 cut(s)	424
AclWI	GGATC	6 cut(s)	179, 866, 1761, 1814, 1895, 1908
AcsI	RAATTY	4 cut(s)	67, 159, 890, 1666
AcuI	CTGAAG	1 cut(s)	1894
AfaI	GTAC	6 cut(s)	15, 371, 899, 953, 1378, 1510
AfiI	CCNNNNNNNGG	3 cut(s)	231, 725, 809
AflIII	ACRYGT	2 cut(s)	900, 1153
AgeI	ACCGGT	1 cut(s)	787
AhlI	ACTAGT	1 cut(s)	1022
AjnI	CCWGG	5 cut(s)	230, 688, 808, 1269, 1351
AjuI	GAANNNNNNNTTGG	4 cut(s)	1583, 1615, 1867, 1899
AleI	CACNNNNGTG	1 cut(s)	348
AluBI	AGCT	9 cut(s)	306, 672, 860, 921, 1078, 1118, 1309, 1588, 1700
AluI	AGCT	9 cut(s)	306, 672, 860, 921, 1078, 1118, 1309, 1588, 1700
Alw21I	GWGCWC	1 cut(s)	308
Alw26I	GTCTC	2 cut(s)	909, 1527
AlwI	GGATC	6 cut(s)	179, 866, 1761, 1814, 1895, 1908
AlwNI	CAGNNNCTG	2 cut(s)	1633, 1917
Aor13HI	TCCGGA	1 cut(s)	424
AoxI	GGCC	7 cut(s)	83, 250, 591, 703, 1272, 1354, 1564
ApeKI	GCWGC	1 cut(s)	1134
ApoI	RAATTY	4 cut(s)	67, 159, 890, 1666
AsiGI	ACCGGT	1 cut(s)	787
AspA2I	CCTAGG	1 cut(s)	1802
AspLEI	GCGC	2 cut(s)	1037, 1771
AspS9I	GGNCC	1 cut(s)	250
AsuC2I	CCSGG	1 cut(s)	1276
AsuHPI	GGTGA	4 cut(s)	66, 514, 1301, 1493
AvrII	CCTAGG	1 cut(s)	1802
BamHI	GGATCC	1 cut(s)	1900
BanII	GRGCYC	2 cut(s)	308, 378
Bbv12I	GWGCWC	1 cut(s)	308
BbvI	GCAGC	1 cut(s)	1121
BccI	CCATC	6 cut(s)	503, 641, 1496, 1543, 1640, 1726
BceAI	ACGGC	2 cut(s)	440, 1377
BcgI	CGANNNNNNTGC	6 cut(s)	277, 311, 952, 986, 1061, 1095
BciT130I	CCWGG	5 cut(s)	232, 690, 810, 1271, 1353
BclI	TGATCA	1 cut(s)	1291
BcnI	CCSGG	1 cut(s)	1276
BcoDI	GTCTC	2 cut(s)	909, 1527
BcuI	ACTAGT	1 cut(s)	1022
BfaI	CTAG	4 cut(s)	53, 1011, 1023, 1803
BfoI	RGCGCY	1 cut(s)	1772
BglI	GCCNNNNNGGC	1 cut(s)	599
BglII	AGATCT	1 cut(s)	1438
BisI	GCNGC	4 cut(s)	594, 1135, 1564, 1631
BlnI	CCTAGG	1 cut(s)	1802
BlsI	GCNGC	4 cut(s)	595, 1136, 1565, 1632
Bme1390I	CCNGG	6 cut(s)	232, 690, 810, 1271, 1276, 1353
BmgT120I	GGNCC	1 cut(s)	250
BmiI	GGNNCC	4 cut(s)	180, 792, 1242, 1902
BmrFI	CCNGG	6 cut(s)	232, 690, 810, 1271, 1276, 1353
BmsI	GCATC	6 cut(s)	724, 772, 960, 1303, 1338, 1630
BoxI	GACNNNNGTC	1 cut(s)	420
BplI	GAGNNNNNCTC	2 cut(s)	905, 937
BpuEI	CTTGAG	2 cut(s)	439, 959
BpuMI	CCSGG	1 cut(s)	1276
Bsa29I	ATCGAT	1 cut(s)	1071
BsaAI	YACGTR	3 cut(s)	478, 493, 901
BsaJI	CCNNGG	7 cut(s)	379, 594, 706, 808, 1195, 1802, 1896
BsaWI	WCCGGW	2 cut(s)	424, 787
Bsc4I	CCNNNNNNNGG	3 cut(s)	231, 725, 809
Bse118I	RCCGGY	2 cut(s)	409, 787
Bse3DI	GCAATG	2 cut(s)	213, 1896
BseAI	TCCGGA	1 cut(s)	424
BseBI	CCWGG	5 cut(s)	232, 690, 810, 1271, 1353
BseCI	ATCGAT	1 cut(s)	1071
BseDI	CCNNGG	7 cut(s)	379, 594, 706, 808, 1195, 1802, 1896
BseGI	GGATG	8 cut(s)	221, 514, 715, 787, 975, 1318, 1408, 1857
BseLI	CCNNNNNNNGG	3 cut(s)	231, 725, 809
BseMI	GCAATG	2 cut(s)	213, 1896
BseMII	CTCAG	2 cut(s)	1076, 1862
BseRI	GAGGAG	3 cut(s)	449, 635, 979
BseXI	GCAGC	1 cut(s)	1121
BseYI	CCCAGC	1 cut(s)	1771
BsgI	GTGCAG	2 cut(s)	500, 1120
Bsh1236I	CGCG	4 cut(s)	596, 1037, 1284, 1472
BshFI	GGCC	7 cut(s)	85, 252, 593, 705, 1274, 1356, 1566
BshTI	ACCGGT	1 cut(s)	787
BshVI	ATCGAT	1 cut(s)	1071
BsiHKAI	GWGCWC	1 cut(s)	308
BsiSI	CCGG	4 cut(s)	410, 425, 788, 1275
BsiWI	CGTACG	1 cut(s)	369
BslFI	GGGAC	1 cut(s)	791
BslI	CCNNNNNNNGG	3 cut(s)	231, 725, 809
BsmAI	GTCTC	2 cut(s)	909, 1527
BsmFI	GGGAC	1 cut(s)	791
BsmI	GAATGC	2 cut(s)	964, 1333
BsnI	GGCC	7 cut(s)	85, 252, 593, 705, 1274, 1356, 1566
Bsp1286I	GDGCHC	2 cut(s)	308, 378
Bsp13I	TCCGGA	1 cut(s)	424
BspANI	GGCC	7 cut(s)	85, 252, 593, 705, 1274, 1356, 1566
BspCNI	CTCAG	2 cut(s)	1077, 1863
BspDI	ATCGAT	1 cut(s)	1071
BspEI	TCCGGA	1 cut(s)	424
BspFNI	CGCG	4 cut(s)	596, 1037, 1284, 1472
BspLI	GGNNCC	4 cut(s)	180, 792, 1242, 1902
BspPI	GGATC	6 cut(s)	179, 866, 1761, 1814, 1895, 1908
BsrDI	GCAATG	2 cut(s)	213, 1896
BsrFI	RCCGGY	2 cut(s)	409, 787
BssAI	RCCGGY	2 cut(s)	409, 787
BssECI	CCNNGG	7 cut(s)	379, 594, 706, 808, 1195, 1802, 1896
BssT1I	CCWWGG	2 cut(s)	1195, 1802
Bst2UI	CCWGG	5 cut(s)	232, 690, 810, 1271, 1353
Bst4CI	ACNGT	9 cut(s)	350, 383, 847, 1126, 1228, 1266, 1765, 1813, 1897
Bst6I	CTCTTC	4 cut(s)	576, 1034, 1104, 1304
BstAPI	GCANNNNNTGC	1 cut(s)	1348
BstBAI	YACGTR	3 cut(s)	478, 493, 901
BstC8I	GCNNGC	2 cut(s)	1080, 1826
BstDEI	CTNAG	3 cut(s)	282, 1085, 1871
BstDSI	CCRYGG	3 cut(s)	379, 594, 1896
BstENI	CCTNNNNNAGG	1 cut(s)	229
BstF5I	GGATG	8 cut(s)	221, 514, 715, 787, 975, 1318, 1408, 1857
BstFNI	CGCG	4 cut(s)	596, 1037, 1284, 1472
BstH2I	RGCGCY	1 cut(s)	1772
BstHHI	GCGC	2 cut(s)	1037, 1771
BstMAI	GTCTC	2 cut(s)	909, 1527
BstMWI	GCNNNNNNNGC	4 cut(s)	599, 1088, 1348, 1630
BstNI	CCWGG	5 cut(s)	232, 690, 810, 1271, 1353
BstNSI	RCATGY	2 cut(s)	101, 1157
BstPAI	GACNNNNGTC	1 cut(s)	420
BstSCI	CCNGG	6 cut(s)	230, 688, 808, 1269, 1274, 1351
BstUI	CGCG	4 cut(s)	596, 1037, 1284, 1472
BstV1I	GCAGC	1 cut(s)	1121
BstX2I	RGATCY	4 cut(s)	871, 1438, 1753, 1900
BstYI	RGATCY	4 cut(s)	871, 1438, 1753, 1900
Bsu15I	ATCGAT	1 cut(s)	1071
BsuRI	GGCC	7 cut(s)	85, 252, 593, 705, 1274, 1356, 1566
BsuTUI	ATCGAT	1 cut(s)	1071
BtgI	CCRYGG	3 cut(s)	379, 594, 1896
BtsCI	GGATG	8 cut(s)	221, 514, 715, 787, 975, 1318, 1408, 1857
BtsIMutI	CAGTG	1 cut(s)	1770
Cac8I	GCNNGC	2 cut(s)	1080, 1826
CaiI	CAGNNNCTG	2 cut(s)	1633, 1917
CfoI	GCGC	2 cut(s)	1037, 1771
Cfr10I	RCCGGY	2 cut(s)	409, 787
Cfr13I	GGNCC	1 cut(s)	250
Cfr42I	CCGCGG	1 cut(s)	597
ClaI	ATCGAT	1 cut(s)	1071
Csp6I	GTAC	6 cut(s)	14, 370, 898, 952, 1377, 1509
CspAI	ACCGGT	1 cut(s)	787
CspCI	CAANNNNNGTGG	2 cut(s)	1878, 1913
CviAII	CATG	9 cut(s)	30, 98, 772, 836, 852, 1154, 1726, 1738, 1818
CviQI	GTAC	6 cut(s)	14, 370, 898, 952, 1377, 1509
DdeI	CTNAG	3 cut(s)	282, 1085, 1871
DraI	TTTAAA	1 cut(s)	1338
Eam1104I	CTCTTC	4 cut(s)	576, 1034, 1104, 1304
EarI	CTCTTC	4 cut(s)	576, 1034, 1104, 1304
EciI	GGCGGA	3 cut(s)	351, 617, 757
Ecl136II	GAGCTC	1 cut(s)	306
Eco130I	CCWWGG	2 cut(s)	1195, 1802
Eco24I	GRGCYC	2 cut(s)	308, 378
Eco53kI	GAGCTC	1 cut(s)	306
Eco57I	CTGAAG	1 cut(s)	1894
EcoICRI	GAGCTC	1 cut(s)	306
EcoNI	CCTNNNNNAGG	1 cut(s)	229
EcoRII	CCWGG	5 cut(s)	230, 688, 808, 1269, 1351
EcoT14I	CCWWGG	2 cut(s)	1195, 1802
EcoT38I	GRGCYC	2 cut(s)	308, 378
ErhI	CCWWGG	2 cut(s)	1195, 1802
FaeI	CATG	9 cut(s)	33, 101, 775, 839, 855, 1157, 1729, 1741, 1821
FalI	AAGNNNNNCTT	2 cut(s)	1425, 1457
FaqI	GGGAC	1 cut(s)	791
FatI	CATG	9 cut(s)	29, 97, 771, 835, 851, 1153, 1725, 1737, 1817
FauI	CCCGC	2 cut(s)	589, 1084
FbaI	TGATCA	1 cut(s)	1291
FblI	GTMKAC	2 cut(s)	287, 414
Fnu4HI	GCNGC	4 cut(s)	594, 1135, 1564, 1631
FokI	GGATG	8 cut(s)	208, 521, 702, 794, 982, 1325, 1415, 1864
FriOI	GRGCYC	2 cut(s)	308, 378
Fsp4HI	GCNGC	4 cut(s)	594, 1135, 1564, 1631
FspBI	CTAG	4 cut(s)	53, 1011, 1023, 1803
GlaI	GCGC	2 cut(s)	1036, 1770
GluI	GCNGC	4 cut(s)	594, 1135, 1564, 1631
GsaI	CCCAGC	1 cut(s)	1775
HaeII	RGCGCY	1 cut(s)	1772
HaeIII	GGCC	7 cut(s)	85, 252, 593, 705, 1274, 1356, 1566
HapII	CCGG	4 cut(s)	410, 425, 788, 1275
HhaI	GCGC	2 cut(s)	1037, 1771
Hin1II	CATG	9 cut(s)	33, 101, 775, 839, 855, 1157, 1729, 1741, 1821
Hin6I	GCGC	2 cut(s)	1035, 1769
HinP1I	GCGC	2 cut(s)	1035, 1769
HincII	GTYRAC	2 cut(s)	192, 288
HindII	GTYRAC	2 cut(s)	192, 288
HindIII	AAGCTT	2 cut(s)	670, 1076
HinfI	GANTC	6 cut(s)	154, 213, 421, 745, 1205, 1914
HpaII	CCGG	4 cut(s)	410, 425, 788, 1275
HphI	GGTGA	4 cut(s)	66, 514, 1301, 1493
Hpy166II	GTNNAC	5 cut(s)	192, 288, 343, 415, 1249
Hpy188I	TCNGA	9 cut(s)	501, 701, 1086, 1214, 1447, 1721, 1758, 1913, 1919
Hpy188III	TCNNGA	8 cut(s)	425, 726, 794, 865, 1526, 1571, 1796, 1904
Hpy8I	GTNNAC	5 cut(s)	192, 288, 343, 415, 1249
Hpy99I	CGWCG	1 cut(s)	292
HpyAV	CCTTC	6 cut(s)	196, 395, 583, 817, 1138, 1577
HpyCH4III	ACNGT	9 cut(s)	350, 383, 847, 1126, 1228, 1266, 1765, 1813, 1897
HpyCH4IV	ACGT	4 cut(s)	185, 477, 492, 900
HpyCH4V	TGCA	6 cut(s)	481, 785, 1058, 1137, 1817, 1824
HpyF10VI	GCNNNNNNNGC	4 cut(s)	599, 1088, 1348, 1630
HpyF3I	CTNAG	3 cut(s)	282, 1085, 1871
HpySE526I	ACGT	4 cut(s)	185, 477, 492, 900
Hsp92II	CATG	9 cut(s)	33, 101, 775, 839, 855, 1157, 1729, 1741, 1821
HspAI	GCGC	2 cut(s)	1035, 1769
Kpn2I	TCCGGA	1 cut(s)	424
Ksp22I	TGATCA	1 cut(s)	1291
KspI	CCGCGG	1 cut(s)	597
LmnI	GCTCC	2 cut(s)	712, 926
Lsp1109I	GCAGC	1 cut(s)	1121
LweI	GCATC	6 cut(s)	724, 772, 960, 1303, 1338, 1630
MaeI	CTAG	4 cut(s)	53, 1011, 1023, 1803
MaeII	ACGT	4 cut(s)	185, 477, 492, 900
MaeIII	GTNAC	3 cut(s)	760, 847, 938
MfeI	CAATTG	1 cut(s)	1842
MflI	RGATCY	4 cut(s)	871, 1438, 1753, 1900
MhlI	GDGCHC	2 cut(s)	308, 378
MlyI	GAGTC	2 cut(s)	222, 430
MmeI	TCCRAC	5 cut(s)	486, 646, 1161, 1581, 1665
MroI	TCCGGA	1 cut(s)	424
MseI	TTAA	3 cut(s)	129, 1188, 1337
MslI	CAYNNNNRTG	2 cut(s)	348, 1730
MspA1I	CMGCKG	2 cut(s)	596, 1633
MspI	CCGG	4 cut(s)	410, 425, 788, 1275
MspR9I	CCNGG	6 cut(s)	232, 690, 810, 1271, 1276, 1353
MunI	CAATTG	1 cut(s)	1842
Mva1269I	GAATGC	2 cut(s)	964, 1333
MvaI	CCWGG	5 cut(s)	232, 690, 810, 1271, 1353
MvnI	CGCG	4 cut(s)	596, 1037, 1284, 1472
MwoI	GCNNNNNNNGC	4 cut(s)	599, 1088, 1348, 1630
NciI	CCSGG	1 cut(s)	1276
NlaIII	CATG	9 cut(s)	33, 101, 775, 839, 855, 1157, 1729, 1741, 1821
NlaIV	GGNNCC	4 cut(s)	180, 792, 1242, 1902
NmeAIII	GCCGAG	1 cut(s)	731
NmuCI	GTSAC	1 cut(s)	938
NspI	RCATGY	2 cut(s)	101, 1157
OliI	CACNNNNGTG	1 cut(s)	348
PciI	ACATGT	1 cut(s)	1153
PcsI	WCGNNNNNNNCGW	1 cut(s)	648
PctI	GAATGC	2 cut(s)	964, 1333
PfeI	GAWTC	4 cut(s)	154, 745, 1205, 1914
Pfl23II	CGTACG	1 cut(s)	369
PfoI	TCCNGGA	1 cut(s)	688
PinAI	ACCGGT	1 cut(s)	787
PkrI	GCNGC	4 cut(s)	595, 1136, 1565, 1632
PleI	GAGTC	2 cut(s)	221, 429
PpsI	GAGTC	2 cut(s)	221, 429
Ppu21I	YACGTR	3 cut(s)	478, 493, 901
PscI	ACATGT	1 cut(s)	1153
PshAI	GACNNNNGTC	1 cut(s)	420
PsiI	TTATAA	1 cut(s)	1464
Psp124BI	GAGCTC	1 cut(s)	308
Psp6I	CCWGG	5 cut(s)	230, 688, 808, 1269, 1351
PspFI	CCCAGC	1 cut(s)	1771
PspGI	CCWGG	5 cut(s)	230, 688, 808, 1269, 1351
PspLI	CGTACG	1 cut(s)	369
PspN4I	GGNNCC	4 cut(s)	180, 792, 1242, 1902
PspPI	GGNCC	1 cut(s)	250
PstNI	CAGNNNCTG	2 cut(s)	1633, 1917
PsuI	RGATCY	4 cut(s)	871, 1438, 1753, 1900
RsaI	GTAC	6 cut(s)	15, 371, 899, 953, 1378, 1510
RsaNI	GTAC	6 cut(s)	14, 370, 898, 952, 1377, 1509
RseI	CAYNNNNRTG	2 cut(s)	348, 1730
SacI	GAGCTC	1 cut(s)	308
SacII	CCGCGG	1 cut(s)	597
SalI	GTCGAC	1 cut(s)	286
SaqAI	TTAA	3 cut(s)	129, 1188, 1337
SatI	GCNGC	4 cut(s)	594, 1135, 1564, 1631
Sau96I	GGNCC	1 cut(s)	250
SchI	GAGTC	2 cut(s)	222, 430
ScrFI	CCNGG	6 cut(s)	232, 690, 810, 1271, 1276, 1353
SduI	GDGCHC	2 cut(s)	308, 378
SfaNI	GCATC	6 cut(s)	724, 772, 960, 1303, 1338, 1630
Sfr303I	CCGCGG	1 cut(s)	597
SgrBI	CCGCGG	1 cut(s)	597
SmiMI	CAYNNNNRTG	2 cut(s)	348, 1730
SmlI	CTYRAG	2 cut(s)	418, 974
SmoI	CTYRAG	2 cut(s)	418, 974
SpeI	ACTAGT	1 cut(s)	1022
SspMI	CTAG	4 cut(s)	53, 1011, 1023, 1803
SstI	GAGCTC	1 cut(s)	308
StyD4I	CCNGG	6 cut(s)	230, 688, 808, 1269, 1274, 1351
StyI	CCWWGG	2 cut(s)	1195, 1802
TaaI	ACNGT	9 cut(s)	350, 383, 847, 1126, 1228, 1266, 1765, 1813, 1897
TaiI	ACGT	4 cut(s)	188, 480, 495, 903
TaqI	TCGA	5 cut(s)	272, 287, 1071, 1208, 1781
TauI	GCSGC	3 cut(s)	596, 1566, 1633
TfiI	GAWTC	4 cut(s)	154, 745, 1205, 1914
Tru1I	TTAA	3 cut(s)	129, 1188, 1337
Tru9I	TTAA	3 cut(s)	129, 1188, 1337
TscAI	CASTG	1 cut(s)	1770
TseFI	GTSAC	1 cut(s)	938
TseI	GCWGC	1 cut(s)	1134
Tsp45I	GTSAC	1 cut(s)	938
TspDTI	ATGAA	5 cut(s)	210, 414, 476, 560, 1113
TspGWI	ACGGA	1 cut(s)	357
TspRI	CASTG	1 cut(s)	1770
XagI	CCTNNNNNAGG	1 cut(s)	229
XapI	RAATTY	4 cut(s)	67, 159, 890, 1666
XceI	RCATGY	2 cut(s)	101, 1157
XcmI	CCANNNNNNNNNTGG	1 cut(s)	534
XmaJI	CCTAGG	1 cut(s)	1802
XmiI	GTMKAC	2 cut(s)	287, 414
XspI	CTAG	4 cut(s)	53, 1011, 1023, 1803

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.