Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0005383

Physical Location & Seq

Genomic Coordinates Reverse (-)

14662087 .. 14674215

Gene Structure 12129 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1176900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2652 bp

ATGGCTGTTCAGACTTCTACTAAACCGAATGTATATGAAGACGAGGATGATGAACCTTTAGTTTTCAAACGGTGCAGCACGAAATCTAAACCCAATCAATTAAACGCCGAAGCAAAGAAAATACCAGCTCAGAGGCAAGTTGGACAACCAGGGAAGCAGGTATCTGAACACCGTTCTTCAAATGGTCTAAACTCGAGTGTTCAAAAAGGTAAGATGGTTCCATCAACCAAATCACCACCAGTAAAATCTCCGCTAGCAAGCCCAAAAGCTTCAACTTCATCTGCTGGGGCATCAGCAGTGTCAAATTCAAAAGCTTCAACTTCATCTAGGGCATCACCAGTACCCAAATCACCACCGTTGACTACATTAGATGGTCATTCTAAACGGTTATCAGAACAGAATAAGTCTACCACAATTAAGAAGGAAATTAATTCTATTGAGCATCCTGCTCAAGGAAATAGTGATTCTGAAGATGAAAAACCGATAGGTGCCAGACTGGGGAAAGTGAAACCTTCTGAAGATTCAGATGATGATAAACCTTTATCATCAAGGATTGCACCAAAGTCCAAGGTGGGAACATCAGGCAGTCACCCTTATGATTCTAAAGAAAGGAAGCCCGTTGTTTCAAAAGTTCAGAAGAACGGTTCCAGCACAACAGATAAAGGCAAAGCTCCATCTGTGGTCTCTAGTAAGAGGCCTCTGGATAAGGCCAATCCTTCTGAGAACTCATCAGCTAAAAGGCTAAAAGTTTCAGATTCTTCTACATCTGTAAAGAATAAGCCGGTAGCGGCCAAACAAGAACCAAAGGAAGAAGATGATGATGATGACTTCATTCCAATTTCCCAGAGGAGTAAGAAAGTTGCATCGGATAGTAAATCATCTCTGACAAAGCAAACCGTAACCAAAGTTGGTTCATTCTCAATCAAGAAAACGATCAAGAAGAGCCAAAAGGCATCAAAATTTACAAAGTATTCTAAATCGACCACGAAGCCACCGAGCTCTAATGATGGGCAGACAAAATGGACAACTTTAGAACACAATGGTGTCATTTTCCCACCTCCATACAAGCCTCATGGGATAAAGATGCTTTATAATGGGAAGCCAGTCACCTTGACTCCTGAACAAGAGGAGGTTGCAACAATGTATGCAGTGATGAAAGATACAGATTATGTGCAAAAAGAAACTTTCAGAAAAAATTTCTGGGCTGATTGGCATAAGTTGCTTGGGAAGAACCATGTAATTCAGAAATTGGATGCTTGTGATTTTACCCCAATATATGACTGGTATCAGAATGAAAAGGAAAAGAAGAAACAAATGAGTACAGAAGAGAAGAAGGCCTTGAAGGAGGAGAAACTAAAACAAGAGGAGAAGTATATGTGGGCTCGCGTTGATGGTAACCAAGAAAAGGTTGGAAACTTCAGAGTTGAACCACCTGGGTTATTCCGAGGTCGTGGAGAGCATCCAAAGTCGGGAAAGCTGAAAAGACGGATTTGTCCAAGTGATGTTACAATTAATATAGGAAAGAATGCCCCAATTCCAGAGTGTCCCATCCCTGGTGAAAGCTGGAAAGAAGTAAGGCATGACAATACGGTCACATGGTTAGCTTTCTGGAACGATCCAATTAATCCAAGGGAATTCAAATACGTGTTTCTGGCACCTAGTAGTAACTTGAAGGGTTTAAGTGACATGCAGAAATACGAAAAAGCACGGAGATTGAAGGACCATATAGGAAAAATCAGGGCTTCATACACCAAGGATTTTACTAGTAAAGATGTAACTAAGCGGCAGATAGCAGTTGCTACCTATCTCATTGATAAACTTGCTCTCAGGGCGGGTAATGAGAAGGATGATGATGAAGCGGATACTGTTGGTTGCTGCACTTTAAAAGTAGAGAATGTAAAAGCAATTCCCCCTAACTCGTTGGAGTTTAACTTCCTTGGTAAAGACTCAATCAGATATGAAAATACTGTTGAAGTTGAGACTCCTGTTTACAAGGCAATTATACAGTTCCAGTCTGGGAAACGTGGCAGTGATGATCTCTTTGACATGTTGGATACCAGTAGATTGAATGCTCATCTAAAGAATGTGATGGATGGCCTTACAGCAAAAGTGTTCCGTACCTACAATGCATCTATCACATTGGATGACATATTGAACAAGAAAACCAAAGACGGAGATCTTTCAGAGAAACTCGTTGTTTATCAGCATGCAAACAAAGAGGTTGCCATCATTTGTAATCATCAACGGACTGTCTCAAAGACTCACAGTGCACAAATGTCCAAGTTAACAGAAAAGATTGATGACCTTCAGGGCATCGTGAAAGATCTGAAAATAGATTTGGACAGGGCCAAAAAAGGAAAGCCCCCACTGAAAGATGCTGATGGAAAGCAAAAGAAAAATTTGACCCCGGAAGCGTTGGAGAGGAAGATAGCTCAAACAAATGCAAAGATTGAAAAAATGGAACTGGATATGAGAACTAAAGAGGATCTGAAAACAGTGGCATTGGGAACGTCTAAAATCAACTATCTTGACCCGAGGATCACCGTTGCTTGGTGCAAGCGGCATGAAGTTCCAATCGAGAAGATCTTCAACAAGTCACTCCTCGCGAAGTTTTCTTGGGCAATGCCAGTGGAGCCTGACTTCAGATTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

884

Amino Acids

98.75

Weight (kDa)

9.53

Isoelectric Point (pI)

37.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Topoisom_I_N	PF02919	340 - 551	1.1e-94	Eukaryotic DNA topoisomerase I, DNA binding fragment
Topoisom_I	PF01028	554 - 777	1.8e-94	Eukaryotic DNA topoisomerase I, catalytic core
Topo_C_assoc	PF14370	815 - 883	6.4e-35	C-terminal topoisomerase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005383)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G55310
fragaria_vesca	FvH4_4g16730 FvH4_4g16730
malus_domestica	MD00G1109500.v1.1 MD13G1176900.v1.1 MD16G1178600.v1.1
prunus_persica	Prupe.1G146000_v2.0.a1 Prupe.1G146000_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1
pyrus_communis	pycom10g21080 pycom13g15260 pycom16g15010
rosa_chinensis	RchiOBHm_Chr4g0420301
rosa_laevigata	RLG00000003998 RLG00000007777 RLG00000007780
rosa_multiflora	Rmu_sc0001767.1_g000005
rosa_roxburghii	Rroxscaffold_5G00362940
rosa_rugosa	Rorug04G0163000 Rorug04G0163100 Rorug04G0163200
rosa_samantha	Rh4AG224100 Rh4BG227100 Rh4CG238200
rosa_wichuraiana	Rw4G019300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1092
AasI	GACNNNNNNGTC	2 cut(s)	1491, 1589
Acc36I	ACCTGC	1 cut(s)	148
AccB1I	GGYRCC	2 cut(s)	488, 1654
AccI	GTMKAC	1 cut(s)	407
AccII	CGCG	2 cut(s)	1386, 2606
AciI	CCGC	6 cut(s)	251, 788, 1783, 1832, 1859, 2560
AclWI	GGATC	3 cut(s)	1610, 2493, 2546
AcoI	YGGCCR	1 cut(s)	789
AcsI	RAATTY	5 cut(s)	304, 959, 1195, 1634, 2398
AcuI	CTGAAG	5 cut(s)	489, 537, 1402, 2291, 2626
AfaI	GTAC	3 cut(s)	342, 1321, 2119
AfiI	CCNNNNNNNGG	6 cut(s)	452, 498, 1405, 1469, 1553, 2550
AflIII	ACRYGT	2 cut(s)	1644, 2046
AhlI	ACTAGT	1 cut(s)	1763
AjnI	CCWGG	3 cut(s)	148, 1432, 1552
AjuI	GAANNNNNNNTTGG	2 cut(s)	2321, 2353
AleI	CACNNNNGTG	1 cut(s)	1041
Alw21I	GWGCWC	2 cut(s)	1001, 2272
Alw26I	GTCTC	3 cut(s)	688, 1973, 2257
Alw44I	GTGCAC	1 cut(s)	2268
AlwI	GGATC	3 cut(s)	1610, 2493, 2546
AlwNI	CAGNNNCTG	1 cut(s)	2649
Ama87I	CYCGRG	2 cut(s)	193, 2533
AoxI	GGCC	6 cut(s)	695, 708, 789, 1335, 2095, 2346
ApaLI	GTGCAC	1 cut(s)	2268
ApeKI	GCWGC	2 cut(s)	75, 1875
ApoI	RAATTY	5 cut(s)	304, 959, 1195, 1634, 2398
AseI	ATTAAT	3 cut(s)	429, 1512, 1623
Asp700I	GAANNNNTTC	1 cut(s)	2585
AspS9I	GGNCC	2 cut(s)	1720, 2346
AsuC2I	CCSGG	1 cut(s)	2408
AsuHPI	GGTGA	7 cut(s)	225, 327, 342, 581, 1099, 1568, 2533
AsuNHI	GCTAGC	1 cut(s)	253
AvaI	CYCGRG	2 cut(s)	193, 2533
AvaII	GGWCC	1 cut(s)	1720
BaeGI	GKGCMC	1 cut(s)	2272
BanI	GGYRCC	2 cut(s)	488, 1654
BanII	GRGCYC	2 cut(s)	1001, 1384
BbsI	GAAGAC	1 cut(s)	45
Bbv12I	GWGCWC	2 cut(s)	1001, 2272
BbvI	GCAGC	2 cut(s)	87, 1862
BciT130I	CCWGG	3 cut(s)	150, 1434, 1554
BciVI	GTATCC	2 cut(s)	1855, 2047
BcnI	CCSGG	1 cut(s)	2408
BcoDI	GTCTC	3 cut(s)	688, 1973, 2257
BcuI	ACTAGT	1 cut(s)	1763
BfaI	CTAG	5 cut(s)	254, 327, 687, 1659, 1764
BfuAI	ACCTGC	1 cut(s)	148
BfuI	GTATCC	2 cut(s)	1855, 2047
BglII	AGATCT	3 cut(s)	2176, 2323, 2583
BisI	GCNGC	5 cut(s)	76, 789, 1784, 1876, 2561
BlsI	GCNGC	5 cut(s)	77, 790, 1785, 1877, 2562
Bme1390I	CCNGG	4 cut(s)	150, 1434, 1554, 2408
Bme18I	GGWCC	1 cut(s)	1720
BmeT110I	CYCGRG	2 cut(s)	193, 2533
BmgT120I	GGNCC	2 cut(s)	1720, 2346
BmiI	GGNNCC	5 cut(s)	219, 490, 646, 1656, 2634
BmrFI	CCNGG	4 cut(s)	150, 1434, 1554, 2408
BmrI	ACTGGG	1 cut(s)	506
BmtI	GCTAGC	1 cut(s)	257
BmuI	ACTGGG	1 cut(s)	506
BpiI	GAAGAC	1 cut(s)	45
BpuEI	CTTGAG	1 cut(s)	435
BpuMI	CCSGG	1 cut(s)	2408
BsaAI	YACGTR	1 cut(s)	1645
BsaI	GGTCTC	1 cut(s)	688
BsaXI	ACNNNNNCTCC	2 cut(s)	1099, 1129
Bsc4I	CCNNNNNNNGG	6 cut(s)	452, 498, 1405, 1469, 1553, 2550
Bse118I	RCCGGY	1 cut(s)	781
Bse1I	ACTGG	9 cut(s)	239, 338, 501, 1103, 1286, 2011, 2058, 2469, 2627
Bse3DI	GCAATG	1 cut(s)	2628
BseBI	CCWGG	3 cut(s)	150, 1434, 1554
BseGI	GGATG	8 cut(s)	52, 442, 1258, 1459, 1548, 1852, 2098, 2149
BseLI	CCNNNNNNNGG	6 cut(s)	452, 498, 1405, 1469, 1553, 2550
BseMI	GCAATG	1 cut(s)	2628
BseMII	CTCAG	3 cut(s)	143, 711, 1840
BseNI	ACTGG	9 cut(s)	239, 338, 501, 1103, 1286, 2011, 2058, 2469, 2627
BseRI	GAGGAG	5 cut(s)	862, 1142, 1361, 1379, 2591
BseSI	GKGCMC	1 cut(s)	2272
BseXI	GCAGC	2 cut(s)	87, 1862
BseYI	CCCAGC	1 cut(s)	284
BsgI	GTGCAG	2 cut(s)	94, 1861
Bsh1236I	CGCG	2 cut(s)	1386, 2606
BshFI	GGCC	6 cut(s)	697, 710, 791, 1337, 2097, 2348
BshNI	GGYRCC	2 cut(s)	488, 1654
BsiHKAI	GWGCWC	2 cut(s)	1001, 2272
BsiHKCI	CYCGRG	2 cut(s)	193, 2533
BsiSI	CCGG	2 cut(s)	782, 2408
BslFI	GGGAC	1 cut(s)	1530
BslI	CCNNNNNNNGG	6 cut(s)	452, 498, 1405, 1469, 1553, 2550
BsmAI	GTCTC	3 cut(s)	688, 1973, 2257
BsmFI	GGGAC	1 cut(s)	1530
BsmI	GAATGC	2 cut(s)	1531, 2074
BsnI	GGCC	6 cut(s)	697, 710, 791, 1337, 2097, 2348
Bso31I	GGTCTC	1 cut(s)	688
BsoBI	CYCGRG	2 cut(s)	193, 2533
Bsp1286I	GDGCHC	3 cut(s)	1001, 1384, 2272
Bsp143I	GATC	8 cut(s)	933, 1615, 2035, 2176, 2323, 2485, 2538, 2583
Bsp68I	TCGCGA	1 cut(s)	2606
BspACI	CCGC	6 cut(s)	251, 788, 1783, 1832, 1859, 2560
BspANI	GGCC	6 cut(s)	697, 710, 791, 1337, 2097, 2348
BspCNI	CTCAG	3 cut(s)	142, 712, 1839
BspFNI	CGCG	2 cut(s)	1386, 2606
BspLI	GGNNCC	5 cut(s)	219, 490, 646, 1656, 2634
BspMI	ACCTGC	1 cut(s)	148
BspOI	GCTAGC	1 cut(s)	257
BspPI	GGATC	3 cut(s)	1610, 2493, 2546
BspQI	GCTCTTC	1 cut(s)	935
BspT107I	GGYRCC	2 cut(s)	488, 1654
BspTNI	GGTCTC	1 cut(s)	688
BsrDI	GCAATG	1 cut(s)	2628
BsrFI	RCCGGY	1 cut(s)	781
BsrI	ACTGG	9 cut(s)	239, 338, 501, 1103, 1286, 2011, 2058, 2469, 2627
BssAI	RCCGGY	1 cut(s)	781
BssMI	GATC	8 cut(s)	933, 1615, 2035, 2176, 2323, 2485, 2538, 2583
BssT1I	CCWWGG	4 cut(s)	567, 1628, 1752, 1936
Bst2UI	CCWGG	3 cut(s)	150, 1434, 1554
Bst6I	CTCTTC	2 cut(s)	935, 1320
BstAPI	GCANNNNNTGC	1 cut(s)	1219
BstBAI	YACGTR	1 cut(s)	1645
BstC8I	GCNNGC	5 cut(s)	255, 259, 1384, 2208, 2558
BstDEI	CTNAG	4 cut(s)	129, 720, 1779, 1826
BstEII	GGTNACC	1 cut(s)	1394
BstENI	CCTNNNNNAGG	1 cut(s)	450
BstF5I	GGATG	8 cut(s)	52, 442, 1258, 1459, 1548, 1852, 2098, 2149
BstFNI	CGCG	2 cut(s)	1386, 2606
BstKTI	GATC	8 cut(s)	936, 1618, 2038, 2179, 2326, 2488, 2541, 2586
BstMAI	GTCTC	3 cut(s)	688, 1973, 2257
BstMBI	GATC	8 cut(s)	933, 1615, 2035, 2176, 2323, 2485, 2538, 2583
BstMWI	GCNNNNNNNGC	3 cut(s)	1219, 1829, 2632
BstNI	CCWGG	3 cut(s)	150, 1434, 1554
BstNSI	RCATGY	3 cut(s)	1690, 2050, 2210
BstPI	GGTNACC	1 cut(s)	1394
BstSCI	CCNGG	4 cut(s)	148, 1432, 1552, 2406
BstSLI	GKGCMC	1 cut(s)	2272
BstUI	CGCG	2 cut(s)	1386, 2606
BstV1I	GCAGC	2 cut(s)	87, 1862
BstV2I	GAAGAC	1 cut(s)	45
BstX2I	RGATCY	4 cut(s)	2176, 2323, 2485, 2583
BstYI	RGATCY	4 cut(s)	2176, 2323, 2485, 2583
BsuI	GTATCC	2 cut(s)	1855, 2047
BsuRI	GGCC	6 cut(s)	697, 710, 791, 1337, 2097, 2348
BtsCI	GGATG	8 cut(s)	52, 442, 1258, 1459, 1548, 1852, 2098, 2149
BtsI	GCAGTG	3 cut(s)	303, 1155, 2035
BtsIMutI	CAGTG	7 cut(s)	303, 1155, 2035, 2272, 2366, 2502, 2634
BtuMI	TCGCGA	1 cut(s)	2606
BveI	ACCTGC	1 cut(s)	148
Cac8I	GCNNGC	5 cut(s)	255, 259, 1384, 2208, 2558
CaiI	CAGNNNCTG	1 cut(s)	2649
Cfr10I	RCCGGY	1 cut(s)	781
Cfr13I	GGNCC	2 cut(s)	1720, 2346
Csp6I	GTAC	3 cut(s)	341, 1320, 2118
CspCI	CAANNNNNGTGG	2 cut(s)	2610, 2645
CviAII	CATG	8 cut(s)	1073, 1235, 1580, 1596, 1687, 2047, 2207, 2564
CviQI	GTAC	3 cut(s)	341, 1320, 2118
DdeI	CTNAG	4 cut(s)	129, 720, 1779, 1826
DpnI	GATC	8 cut(s)	935, 1617, 2037, 2178, 2325, 2487, 2540, 2585
DpnII	GATC	8 cut(s)	933, 1615, 2035, 2176, 2323, 2485, 2538, 2583
DraI	TTTAAA	1 cut(s)	1884
DrdI	GACNNNNNNGTC	2 cut(s)	1491, 1589
DseDI	GACNNNNNNGTC	2 cut(s)	1491, 1589
EaeI	YGGCCR	1 cut(s)	789
Eam1104I	CTCTTC	2 cut(s)	935, 1320
EarI	CTCTTC	2 cut(s)	935, 1320
Ecl136II	GAGCTC	1 cut(s)	999
Eco130I	CCWWGG	4 cut(s)	567, 1628, 1752, 1936
Eco147I	AGGCCT	2 cut(s)	697, 1337
Eco24I	GRGCYC	2 cut(s)	1001, 1384
Eco31I	GGTCTC	1 cut(s)	688
Eco47I	GGWCC	1 cut(s)	1720
Eco53kI	GAGCTC	1 cut(s)	999
Eco57I	CTGAAG	5 cut(s)	489, 537, 1402, 2291, 2626
Eco88I	CYCGRG	2 cut(s)	193, 2533
Eco91I	GGTNACC	1 cut(s)	1394
EcoICRI	GAGCTC	1 cut(s)	999
EcoNI	CCTNNNNNAGG	1 cut(s)	450
EcoO65I	GGTNACC	1 cut(s)	1394
EcoRI	GAATTC	1 cut(s)	1634
EcoRII	CCWGG	3 cut(s)	148, 1432, 1552
EcoT14I	CCWWGG	4 cut(s)	567, 1628, 1752, 1936
EcoT22I	ATGCAT	1 cut(s)	2131
EcoT38I	GRGCYC	2 cut(s)	1001, 1384
ErhI	CCWWGG	4 cut(s)	567, 1628, 1752, 1936
FaeI	CATG	8 cut(s)	1076, 1238, 1583, 1599, 1690, 2050, 2210, 2567
FalI	AAGNNNNNCTT	2 cut(s)	1322, 1354
FaqI	GGGAC	1 cut(s)	1530
FatI	CATG	8 cut(s)	1072, 1234, 1579, 1595, 1686, 2046, 2206, 2563
FauI	CCCGC	1 cut(s)	1825
FblI	GTMKAC	1 cut(s)	407
Fnu4HI	GCNGC	5 cut(s)	76, 789, 1784, 1876, 2561
FokI	GGATG	8 cut(s)	59, 429, 1265, 1446, 1535, 1859, 2105, 2156
FriOI	GRGCYC	2 cut(s)	1001, 1384
Fsp4HI	GCNGC	5 cut(s)	76, 789, 1784, 1876, 2561
FspBI	CTAG	5 cut(s)	254, 327, 687, 1659, 1764
GluI	GCNGC	5 cut(s)	76, 789, 1784, 1876, 2561
GsaI	CCCAGC	1 cut(s)	288
HaeIII	GGCC	6 cut(s)	697, 710, 791, 1337, 2097, 2348
HapII	CCGG	2 cut(s)	782, 2408
Hin1II	CATG	8 cut(s)	1076, 1238, 1583, 1599, 1690, 2050, 2210, 2567
HincII	GTYRAC	2 cut(s)	360, 2286
HindII	GTYRAC	2 cut(s)	360, 2286
HindIII	AAGCTT	2 cut(s)	267, 312
HinfI	GANTC	9 cut(s)	464, 521, 599, 755, 1114, 1946, 1981, 2260, 2646
HpaI	GTTAAC	1 cut(s)	2286
HpaII	CCGG	2 cut(s)	782, 2408
HphI	GGTGA	7 cut(s)	225, 327, 342, 581, 1099, 1568, 2533
Hpy166II	GTNNAC	5 cut(s)	360, 408, 1990, 2270, 2286
Hpy8I	GTNNAC	5 cut(s)	360, 408, 1990, 2270, 2286
HpyAV	CCTTC	9 cut(s)	415, 522, 726, 1327, 1336, 1666, 1711, 1837, 2315
HpyCH4IV	ACGT	3 cut(s)	1644, 2023, 2510
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1219, 1829, 2632
HpyF3I	CTNAG	4 cut(s)	129, 720, 1779, 1826
HpySE526I	ACGT	3 cut(s)	1644, 2023, 2510
Hsp92II	CATG	8 cut(s)	1076, 1238, 1583, 1599, 1690, 2050, 2210, 2567
KspAI	GTTAAC	1 cut(s)	2286
Kzo9I	GATC	8 cut(s)	933, 1615, 2035, 2176, 2323, 2485, 2538, 2583
LguI	GCTCTTC	1 cut(s)	935
LmnI	GCTCC	2 cut(s)	676, 2632
Lsp1109I	GCAGC	2 cut(s)	87, 1862
MaeI	CTAG	5 cut(s)	254, 327, 687, 1659, 1764
MaeII	ACGT	3 cut(s)	1644, 2023, 2510
MalI	GATC	8 cut(s)	935, 1617, 2037, 2178, 2325, 2487, 2540, 2585
MboI	GATC	8 cut(s)	933, 1615, 2035, 2176, 2323, 2485, 2538, 2583
MflI	RGATCY	4 cut(s)	2176, 2323, 2485, 2583
MhlI	GDGCHC	3 cut(s)	1001, 1384, 2272
MlyI	GAGTC	4 cut(s)	1108, 1940, 1975, 2254
MmeI	TCCRAC	5 cut(s)	121, 1390, 1902, 2031, 2397
Mph1103I	ATGCAT	1 cut(s)	2131
MroXI	GAANNNNTTC	1 cut(s)	2585
MseI	TTAA	9 cut(s)	101, 417, 429, 1512, 1623, 1679, 1883, 1929, 2285
MslI	CAYNNNNRTG	2 cut(s)	594, 1041
MspI	CCGG	2 cut(s)	782, 2408
MspR9I	CCNGG	4 cut(s)	150, 1434, 1554, 2408
Mva1269I	GAATGC	2 cut(s)	1531, 2074
MvaI	CCWGG	3 cut(s)	150, 1434, 1554
MvnI	CGCG	2 cut(s)	1386, 2606
MwoI	GCNNNNNNNGC	3 cut(s)	1219, 1829, 2632
NciI	CCSGG	1 cut(s)	2408
NdeII	GATC	8 cut(s)	933, 1615, 2035, 2176, 2323, 2485, 2538, 2583
NheI	GCTAGC	1 cut(s)	253
NlaIII	CATG	8 cut(s)	1076, 1238, 1583, 1599, 1690, 2050, 2210, 2567
NlaIV	GGNNCC	5 cut(s)	219, 490, 646, 1656, 2634
NmuCI	GTSAC	5 cut(s)	587, 1105, 1591, 1682, 2595
NruI	TCGCGA	1 cut(s)	2606
NsiI	ATGCAT	1 cut(s)	2131
NspI	RCATGY	3 cut(s)	1690, 2050, 2210
OliI	CACNNNNGTG	1 cut(s)	1041
PaeI	GCATGC	1 cut(s)	2210
PaeR7I	CTCGAG	1 cut(s)	193
PceI	AGGCCT	2 cut(s)	697, 1337
PciI	ACATGT	1 cut(s)	2046
PciSI	GCTCTTC	1 cut(s)	935
PcsI	WCGNNNNNNNCGW	1 cut(s)	992
PctI	GAATGC	2 cut(s)	1531, 2074
PdmI	GAANNNNTTC	1 cut(s)	2585
PfeI	GAWTC	5 cut(s)	464, 521, 599, 755, 2646
PkrI	GCNGC	5 cut(s)	77, 790, 1785, 1877, 2562
PleI	GAGTC	4 cut(s)	1108, 1940, 1975, 2254
PpsI	GAGTC	4 cut(s)	1108, 1940, 1975, 2254
Ppu21I	YACGTR	1 cut(s)	1645
PscI	ACATGT	1 cut(s)	2046
PshBI	ATTAAT	3 cut(s)	429, 1512, 1623
PsiI	TTATAA	1 cut(s)	1092
Psp124BI	GAGCTC	1 cut(s)	1001
Psp6I	CCWGG	3 cut(s)	148, 1432, 1552
PspEI	GGTNACC	1 cut(s)	1394
PspFI	CCCAGC	1 cut(s)	284
PspGI	CCWGG	3 cut(s)	148, 1432, 1552
PspN4I	GGNNCC	5 cut(s)	219, 490, 646, 1656, 2634
PspPI	GGNCC	2 cut(s)	1720, 2346
PspXI	VCTCGAGB	1 cut(s)	193
PstNI	CAGNNNCTG	1 cut(s)	2649
PsuI	RGATCY	4 cut(s)	2176, 2323, 2485, 2583
RruI	TCGCGA	1 cut(s)	2606
RsaI	GTAC	3 cut(s)	342, 1321, 2119
RsaNI	GTAC	3 cut(s)	341, 1320, 2118
RseI	CAYNNNNRTG	2 cut(s)	594, 1041
SacI	GAGCTC	1 cut(s)	1001
SapI	GCTCTTC	1 cut(s)	935
SaqAI	TTAA	9 cut(s)	101, 417, 429, 1512, 1623, 1679, 1883, 1929, 2285
SatI	GCNGC	5 cut(s)	76, 789, 1784, 1876, 2561
Sau3AI	GATC	8 cut(s)	933, 1615, 2035, 2176, 2323, 2485, 2538, 2583
Sau96I	GGNCC	2 cut(s)	1720, 2346
SchI	GAGTC	4 cut(s)	1108, 1940, 1975, 2254
ScrFI	CCNGG	4 cut(s)	150, 1434, 1554, 2408
SduI	GDGCHC	3 cut(s)	1001, 1384, 2272
Sfr274I	CTCGAG	1 cut(s)	193
SinI	GGWCC	1 cut(s)	1720
SlaI	CTCGAG	1 cut(s)	193
SmiMI	CAYNNNNRTG	2 cut(s)	594, 1041
SmlI	CTYRAG	2 cut(s)	193, 450
SmoI	CTYRAG	2 cut(s)	193, 450
SpeI	ACTAGT	1 cut(s)	1763
SphI	GCATGC	1 cut(s)	2210
SseBI	AGGCCT	2 cut(s)	697, 1337
SsiI	CCGC	6 cut(s)	251, 788, 1783, 1832, 1859, 2560
SspMI	CTAG	5 cut(s)	254, 327, 687, 1659, 1764
SstI	GAGCTC	1 cut(s)	1001
StuI	AGGCCT	2 cut(s)	697, 1337
StyD4I	CCNGG	4 cut(s)	148, 1432, 1552, 2406
StyI	CCWWGG	4 cut(s)	567, 1628, 1752, 1936
TaiI	ACGT	3 cut(s)	1647, 2026, 2513
TaqI	TCGA	3 cut(s)	194, 980, 2577
TatI	WGTACW	1 cut(s)	1319
TauI	GCSGC	3 cut(s)	791, 1786, 2563
TfiI	GAWTC	5 cut(s)	464, 521, 599, 755, 2646
Tru1I	TTAA	9 cut(s)	101, 417, 429, 1512, 1623, 1679, 1883, 1929, 2285
Tru9I	TTAA	9 cut(s)	101, 417, 429, 1512, 1623, 1679, 1883, 1929, 2285
TscAI	CASTG	7 cut(s)	303, 1155, 2035, 2272, 2373, 2502, 2634
TseFI	GTSAC	5 cut(s)	587, 1105, 1591, 1682, 2595
TseI	GCWGC	2 cut(s)	75, 1875
Tsp45I	GTSAC	5 cut(s)	587, 1105, 1591, 1682, 2595
TspGWI	ACGGA	5 cut(s)	1501, 1723, 2105, 2187, 2260
TspRI	CASTG	7 cut(s)	303, 1155, 2035, 2272, 2373, 2502, 2634
VneI	GTGCAC	1 cut(s)	2268
VpaK11BI	GGWCC	1 cut(s)	1720
VspI	ATTAAT	3 cut(s)	429, 1512, 1623
XagI	CCTNNNNNAGG	1 cut(s)	450
XapI	RAATTY	5 cut(s)	304, 959, 1195, 1634, 2398
XceI	RCATGY	3 cut(s)	1690, 2050, 2210
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1278, 1406
XhoI	CTCGAG	1 cut(s)	193
XmiI	GTMKAC	1 cut(s)	407
XmnI	GAANNNNTTC	1 cut(s)	2585
XspI	CTAG	5 cut(s)	254, 327, 687, 1659, 1764
Zsp2I	ATGCAT	1 cut(s)	2131

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.