Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005074

Physical Location & Seq

Genomic Coordinates Forward (+)

22936534 .. 22939060

Gene Structure 2527 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G56680.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1719 bp

ATGGCTGATGAGATTGTGCCGCCGGCGACTCAATTAGCCGCCGTATCCTTAGAAAACGATGGATCTACGGTGCAAAGAGCTCAATTCTCTAACCGGGTTTTGATTCGAACGATTTTGGATCGACCTGACGGCGGAGCTGGACTCGCCGGTCAAACGGTTCGAATCGGTGGTTGGGTAAAATCCGGAAGAGATCAAGGGAAAAGAACGTTTTCTTTCCTTGCGGTTAACGATGGATCTTGTCCAGCGAATCTTCAGGTTATGGTGGATCCGTCTCTCTATGATGTCTCTAACTTGGTTGCTACGGGAACTTGTGTGACTGTTGATGGAGTTTTGAAGGTTCCACCGAAAGGTAAAGGTACGCAGCAGCAGATTGAGCTTAACGTTGTGAAGGTGATTGATGTTGGAACGGTGGATGCGTCTAAGTATCCACTTCCTAAGACTAAGTTGACTCTTGAGACTTTGAGAGATGTGCTTCATCTTCGTTCTAGAACTAACTCGATCTCAGCTGTTGCAAGAATTCGTAATGCTCTTGCTTTTGCGACTCACAGTTTCTTTCAAGAGCATAGTTTCCTTTACATTCACACTCCTATCATCACGACAAGTGATTGTGAAGGTGCTGGTGAAATGTTCCAAGCAACAACTTTGATCAATTACACTGAGAGGTTGGAGCAGGATCTCATTGATAACCCTCCGCCTACTGAGGCTGATGTTGAAGCTGCGAGGCTCATTGTCATTGAGAGAGGTAATGTTGTAGCGGAACTGAAAGCTGCCAAAGCAAGCAAGGAAGCGATCACTGCTGCTGTTGCTGAACTTAAAATAGCAAAGGAGACTTTTGCTCATATTGATGAGAGGTCGAGGCTTAGACCTGGACTACCTAAGAAAGATGGAAACATTGATTATTCCAAGGATTTCTTTGGTCGTCAAGCTTTCTTGACAGTTTCCGGTCAATTACAAGTGGAGACCTATGCTTGTGCTCTTAGCAATGTGTATACATTTGGCCCGACTTTCCGGGCAGAGAACTCTCACACTTCAAGGCATCTTGCTGAATTCTGGATGGTTGAGCCTGAGATTGCTTTTGCAGATCTAGAGGACGACATGAACTGTGCAGAGGCGTATGTGAAATACATGTGCAACTGGTTGCTTGAGAAATGTTACGCTGACATGGAACTTATGGCTAAGAATTTCGACTCAGGATGCATTGACAGGCTAAAATTGGTTGCCTCTACTCCGTTTGGGCGTATAACATACACTAAAGCAATAGAGCTACTTGAGGAAGCTGTGGCTAAAGGAAAGGAATTTGATAACAATGTGGAGTGGGGAATCGACTTAGCCTCGGAGCATGAAAGATACTTGACAGAGGTTCTGTTTCAAAAGCCACTTATTGTCTATAACTACCCGAAAGGAATCAAAGCTTTTTACATGAGACTTAACGATGATGAGAAGACAGTTGCTGCCATGGATGTCCTCGTTCCAAAGGTTGGAGAACTCATTGGTGGAAGCCAAAGGGAAGAACGATATGATGTCATCAAAAAGAGGATTGAGGAGATGGGTCTTCCAATAGAGCCATATGAGTGGTACCTAGACTTGCGCCGTTATGGAACAGTGAAGCATTGTGGGTTTGGACTTGGCTTCGAACGTATGATTCTCTTCGCTACTGGACTTGATAACATCAGAGACGTTATTCCTTTCCCTCGCTATCCTGGAAAAGCTGATCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0007275 GO:0008150 GO:0009507 GO:0009536 GO:0009790 GO:0009791 GO:0009793 GO:0010035 GO:0010038 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

WHEP-TRS tRNA-synt_2 tRNA_anti-codon

Protein Analysis

572

Amino Acids

63.78

Weight (kDa)

5.4

Isoelectric Point (pI)

34.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA_anti-codon	PF01336	53 - 129	2.5e-09	OB-fold nucleic acid binding domain
WHEP-TRS	PF00458	245 - 279	5.1e-06	WHEP-TRS domain
tRNA-synt_2	PF00152	303 - 565	2.5e-52	tRNA synthetases class II (D, K and N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005074)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70980 AT5G56680
fragaria_vesca	FvH4_3g17430
malus_domestica	MD01G1115900.v1.1 MD03G1251800.v1.1 MD05G1158600.v1.1 MD11G1273000.v1.1
prunus_persica	Prupe.4G154500_v2.0.a1 Prupe.4G154500_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1
pyrus_communis	pycom03g19910 pycom05g15060 pycom11g24090
rosa_chinensis	RchiOBHm_Chr5g0028961
rosa_laevigata	RLG00000033131
rosa_multiflora	Rmu_co7984584.1_g000001 Rmu_sc0009042.1_g000005
rosa_roxburghii	Rroxscaffold_1G00050930
rosa_rugosa	Rorug05G0111900 Rorug05G0112000 Rorug05G0112000
rosa_samantha	Rh5AG204000 Rh5BG202300 Rh5CG223100 Rh5DG206100
rosa_wichuraiana	Rw5G018490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	147
Acc65I	GGTACC	1 cut(s)	1575
AccB1I	GGYRCC	1 cut(s)	1575
AccB7I	CCANNNNNTGG	1 cut(s)	1478
AccI	GTMKAC	1 cut(s)	989
AccIII	TCCGGA	1 cut(s)	182
AciI	CCGC	6 cut(s)	20, 39, 132, 221, 692, 755
AclI	AACGTT	2 cut(s)	206, 381
AclWI	GGATC	6 cut(s)	70, 126, 241, 260, 273, 681
AcsI	RAATTY	4 cut(s)	516, 1046, 1180, 1295
AcuI	CTGAAG	1 cut(s)	236
AfaI	GTAC	2 cut(s)	358, 1577
AfiI	CCNNNNNNNGG	3 cut(s)	131, 347, 1478
AflIII	ACRYGT	1 cut(s)	1125
AgsI	TTSAA	5 cut(s)	334, 557, 713, 1032, 1370
AjnI	CCWGG	2 cut(s)	865, 1699
AjuI	GAANNNNNNNTTGG	2 cut(s)	154, 186
Alw21I	GWGCWC	2 cut(s)	82, 976
Alw26I	GTCTC	7 cut(s)	276, 289, 449, 821, 953, 1417, 1668
AlwI	GGATC	6 cut(s)	70, 126, 241, 260, 273, 681
AlwNI	CAGNNNCTG	1 cut(s)	1451
Aor13HI	TCCGGA	1 cut(s)	182
AoxI	GGCC	1 cut(s)	997
ApeKI	GCWGC	6 cut(s)	361, 364, 716, 767, 797, 1451
ApoI	RAATTY	4 cut(s)	516, 1046, 1180, 1295
Asp700I	GAANNNNTTC	1 cut(s)	208
Asp718I	GGTACC	1 cut(s)	1575
AspLEI	GCGC	1 cut(s)	1590
AspS9I	GGNCC	1 cut(s)	998
AsuC2I	CCSGG	2 cut(s)	95, 1010
AsuHPI	GGTGA	2 cut(s)	403, 632
AsuII	TTCGAA	3 cut(s)	106, 160, 1632
BamHI	GGATCC	1 cut(s)	265
BanI	GGYRCC	1 cut(s)	1575
BanII	GRGCYC	1 cut(s)	82
BbsI	GAAGAC	2 cut(s)	1448, 1544
Bbv12I	GWGCWC	2 cut(s)	82, 976
BbvI	GCAGC	6 cut(s)	373, 376, 703, 754, 784, 1438
BccI	CCATC	6 cut(s)	53, 224, 317, 878, 1048, 1540
BceAI	ACGGC	3 cut(s)	26, 145, 1575
BciT130I	CCWGG	2 cut(s)	867, 1701
BciVI	GTATCC	2 cut(s)	55, 435
BclI	TGATCA	1 cut(s)	645
BcnI	CCSGG	2 cut(s)	95, 1010
BcoDI	GTCTC	7 cut(s)	276, 289, 449, 821, 953, 1417, 1668
BfaI	CTAG	3 cut(s)	486, 1085, 1580
BfuI	GTATCC	2 cut(s)	55, 435
BglII	AGATCT	1 cut(s)	1081
BisI	GCNGC	8 cut(s)	20, 39, 362, 365, 717, 768, 798, 1452
BlsI	GCNGC	8 cut(s)	21, 40, 363, 366, 718, 769, 799, 1453
Bme1390I	CCNGG	4 cut(s)	95, 867, 1010, 1701
BmgT120I	GGNCC	1 cut(s)	998
BmiI	GGNNCC	3 cut(s)	267, 339, 1577
BmrFI	CCNGG	4 cut(s)	95, 867, 1010, 1701
BmsI	GCATC	3 cut(s)	403, 1045, 1184
BpiI	GAAGAC	2 cut(s)	1448, 1544
BplI	GAGNNNNNCTC	2 cut(s)	126, 158
Bpu14I	TTCGAA	3 cut(s)	106, 160, 1632
BpuEI	CTTGAG	3 cut(s)	473, 1163, 1289
BpuMI	CCSGG	2 cut(s)	95, 1010
BsaI	GGTCTC	1 cut(s)	953
BsaJI	CCNNGG	3 cut(s)	903, 1332, 1455
BsaWI	WCCGGW	2 cut(s)	182, 941
Bsc4I	CCNNNNNNNGG	3 cut(s)	131, 347, 1478
Bse118I	RCCGGY	2 cut(s)	22, 146
Bse1I	ACTGG	2 cut(s)	1139, 1660
Bse3DI	GCAATG	1 cut(s)	988
BseAI	TCCGGA	1 cut(s)	182
BseBI	CCWGG	2 cut(s)	867, 1701
BseDI	CCNNGG	3 cut(s)	903, 1332, 1455
BseGI	GGATG	4 cut(s)	418, 1059, 1199, 1465
BseLI	CCNNNNNNNGG	3 cut(s)	131, 347, 1478
BseMI	GCAATG	1 cut(s)	988
BseMII	CTCAG	5 cut(s)	516, 648, 690, 1056, 1203
BseNI	ACTGG	2 cut(s)	1139, 1660
BseRI	GAGGAG	1 cut(s)	1556
BseXI	GCAGC	6 cut(s)	373, 376, 703, 754, 784, 1438
BsgI	GTGCAG	1 cut(s)	1125
BshFI	GGCC	1 cut(s)	999
BshNI	GGYRCC	1 cut(s)	1575
BsiHKAI	GWGCWC	2 cut(s)	82, 976
BsiSI	CCGG	6 cut(s)	23, 94, 147, 183, 942, 1009
BslI	CCNNNNNNNGG	3 cut(s)	131, 347, 1478
BsmAI	GTCTC	7 cut(s)	276, 289, 449, 821, 953, 1417, 1668
BsmBI	CGTCTC	2 cut(s)	276, 1668
BsnI	GGCC	1 cut(s)	999
Bso31I	GGTCTC	1 cut(s)	953
Bsp119I	TTCGAA	3 cut(s)	106, 160, 1632
Bsp1286I	GDGCHC	2 cut(s)	82, 976
Bsp13I	TCCGGA	1 cut(s)	182
Bsp19I	CCATGG	1 cut(s)	1455
BspACI	CCGC	6 cut(s)	20, 39, 132, 221, 692, 755
BspANI	GGCC	1 cut(s)	999
BspCNI	CTCAG	5 cut(s)	515, 649, 691, 1057, 1202
BspEI	TCCGGA	1 cut(s)	182
BspLI	GGNNCC	3 cut(s)	267, 339, 1577
BspPI	GGATC	6 cut(s)	70, 126, 241, 260, 273, 681
BspT104I	TTCGAA	3 cut(s)	106, 160, 1632
BspT107I	GGYRCC	1 cut(s)	1575
BspTNI	GGTCTC	1 cut(s)	953
BsrDI	GCAATG	1 cut(s)	988
BsrFI	RCCGGY	2 cut(s)	22, 146
BsrI	ACTGG	2 cut(s)	1139, 1660
BssAI	RCCGGY	2 cut(s)	22, 146
BssECI	CCNNGG	3 cut(s)	903, 1332, 1455
BssNAI	GTATAC	1 cut(s)	990
BssT1I	CCWWGG	2 cut(s)	903, 1455
Bst1107I	GTATAC	1 cut(s)	990
Bst2UI	CCWGG	2 cut(s)	867, 1701
Bst4CI	ACNGT	9 cut(s)	70, 157, 319, 409, 548, 937, 1103, 1447, 1603
Bst6I	CTCTTC	2 cut(s)	181, 1652
BstBI	TTCGAA	3 cut(s)	106, 160, 1632
BstC8I	GCNNGC	2 cut(s)	24, 778
BstDSI	CCRYGG	1 cut(s)	1455
BstF5I	GGATG	4 cut(s)	418, 1059, 1199, 1465
BstHHI	GCGC	1 cut(s)	1590
BstMAI	GTCTC	7 cut(s)	276, 289, 449, 821, 953, 1417, 1668
BstMWI	GCNNNNNNNGC	5 cut(s)	143, 373, 773, 794, 803
BstNI	CCWGG	2 cut(s)	867, 1701
BstNSI	RCATGY	1 cut(s)	1129
BstSCI	CCNGG	4 cut(s)	93, 865, 1008, 1699
BstV1I	GCAGC	6 cut(s)	373, 376, 703, 754, 784, 1438
BstV2I	GAAGAC	2 cut(s)	1448, 1544
BstX2I	RGATCY	5 cut(s)	62, 233, 265, 673, 1081
BstXI	CCANNNNNNTGG	1 cut(s)	1572
BstYI	RGATCY	5 cut(s)	62, 233, 265, 673, 1081
BstZ17I	GTATAC	1 cut(s)	990
BsuI	GTATCC	2 cut(s)	55, 435
BsuRI	GGCC	1 cut(s)	999
BtgI	CCRYGG	1 cut(s)	1455
BtsCI	GGATG	4 cut(s)	418, 1059, 1199, 1465
BtsI	GCAGTG	1 cut(s)	792
BtsIMutI	CAGTG	3 cut(s)	654, 792, 1608
Cac8I	GCNNGC	2 cut(s)	24, 778
CaiI	CAGNNNCTG	1 cut(s)	1451
CfoI	GCGC	1 cut(s)	1590
Cfr10I	RCCGGY	2 cut(s)	22, 146
Cfr13I	GGNCC	1 cut(s)	998
CseI	GACGC	1 cut(s)	405
Csp6I	GTAC	2 cut(s)	357, 1576
CviAII	CATG	6 cut(s)	1096, 1126, 1162, 1340, 1420, 1456
CviQI	GTAC	2 cut(s)	357, 1576
DrdI	GACNNNNNNGTC	1 cut(s)	147
DseDI	GACNNNNNNGTC	1 cut(s)	147
Eam1104I	CTCTTC	2 cut(s)	181, 1652
EarI	CTCTTC	2 cut(s)	181, 1652
EciI	GGCGGA	2 cut(s)	147, 681
Ecl136II	GAGCTC	1 cut(s)	80
Eco130I	CCWWGG	2 cut(s)	903, 1455
Eco24I	GRGCYC	1 cut(s)	82
Eco31I	GGTCTC	1 cut(s)	953
Eco53kI	GAGCTC	1 cut(s)	80
Eco57I	CTGAAG	1 cut(s)	236
EcoICRI	GAGCTC	1 cut(s)	80
EcoRI	GAATTC	2 cut(s)	516, 1046
EcoRII	CCWGG	2 cut(s)	865, 1699
EcoT14I	CCWWGG	2 cut(s)	903, 1455
EcoT22I	ATGCAT	1 cut(s)	1199
EcoT38I	GRGCYC	1 cut(s)	82
ErhI	CCWWGG	2 cut(s)	903, 1455
Esp3I	CGTCTC	2 cut(s)	276, 1668
FaeI	CATG	6 cut(s)	1099, 1129, 1165, 1343, 1423, 1459
FalI	AAGNNNNNCTT	3 cut(s)	896, 928, 1698
FatI	CATG	6 cut(s)	1095, 1125, 1161, 1339, 1419, 1455
FauNDI	CATATG	1 cut(s)	1567
FbaI	TGATCA	1 cut(s)	645
FblI	GTMKAC	1 cut(s)	989
Fnu4HI	GCNGC	8 cut(s)	20, 39, 362, 365, 717, 768, 798, 1452
FokI	GGATG	4 cut(s)	425, 1066, 1206, 1472
FriOI	GRGCYC	1 cut(s)	82
Fsp4HI	GCNGC	8 cut(s)	20, 39, 362, 365, 717, 768, 798, 1452
FspBI	CTAG	3 cut(s)	486, 1085, 1580
GlaI	GCGC	1 cut(s)	1589
GluI	GCNGC	8 cut(s)	20, 39, 362, 365, 717, 768, 798, 1452
HaeIII	GGCC	1 cut(s)	999
HapII	CCGG	6 cut(s)	23, 94, 147, 183, 942, 1009
HgaI	GACGC	1 cut(s)	405
HhaI	GCGC	1 cut(s)	1590
Hin1II	CATG	6 cut(s)	1099, 1129, 1165, 1343, 1423, 1459
Hin6I	GCGC	1 cut(s)	1588
HinP1I	GCGC	1 cut(s)	1588
HincII	GTYRAC	2 cut(s)	226, 447
HindII	GTYRAC	2 cut(s)	226, 447
HindIII	AAGCTT	2 cut(s)	924, 1410
HpaI	GTTAAC	1 cut(s)	226
HpaII	CCGG	6 cut(s)	23, 94, 147, 183, 942, 1009
HphI	GGTGA	2 cut(s)	403, 632
Hpy166II	GTNNAC	3 cut(s)	226, 447, 990
Hpy188I	TCNGA	2 cut(s)	1336, 1673
Hpy188III	TCNNGA	9 cut(s)	183, 452, 486, 557, 595, 931, 1051, 1085, 1191
Hpy8I	GTNNAC	3 cut(s)	226, 447, 990
HpyAV	CCTTC	3 cut(s)	328, 382, 605
HpyCH4III	ACNGT	9 cut(s)	70, 157, 319, 409, 548, 937, 1103, 1447, 1603
HpyCH4IV	ACGT	4 cut(s)	206, 381, 1636, 1677
HpyCH4V	TGCA	6 cut(s)	73, 512, 1079, 1106, 1131, 1197
HpyF10VI	GCNNNNNNNGC	5 cut(s)	143, 373, 773, 794, 803
HpySE526I	ACGT	4 cut(s)	206, 381, 1636, 1677
Hsp92II	CATG	6 cut(s)	1099, 1129, 1165, 1343, 1423, 1459
HspAI	GCGC	1 cut(s)	1588
Kpn2I	TCCGGA	1 cut(s)	182
KpnI	GGTACC	1 cut(s)	1579
KroI	GCCGGC	1 cut(s)	22
KroNI	GCCGGC	1 cut(s)	24
Ksp22I	TGATCA	1 cut(s)	645
KspAI	GTTAAC	1 cut(s)	226
LmnI	GCTCC	3 cut(s)	134, 667, 1336
Lsp1109I	GCAGC	6 cut(s)	373, 376, 703, 754, 784, 1438
LweI	GCATC	3 cut(s)	403, 1045, 1184
MaeI	CTAG	3 cut(s)	486, 1085, 1580
MaeII	ACGT	4 cut(s)	206, 381, 1636, 1677
MaeIII	GTNAC	2 cut(s)	313, 1151
MboII	GAAGA	7 cut(s)	198, 242, 470, 1453, 1520, 1544, 1639
MflI	RGATCY	5 cut(s)	62, 233, 265, 673, 1081
MhlI	GDGCHC	2 cut(s)	82, 976
MluCI	AATT	9 cut(s)	32, 83, 516, 649, 947, 1046, 1180, 1211, 1295
MlyI	GAGTC	5 cut(s)	22, 135, 442, 535, 1181
MmeI	TCCRAC	3 cut(s)	382, 645, 1459
Mph1103I	ATGCAT	1 cut(s)	1199
MreI	CGCCGGCG	1 cut(s)	22
MroI	TCCGGA	1 cut(s)	182
MroNI	GCCGGC	1 cut(s)	22
MroXI	GAANNNNTTC	1 cut(s)	208
MseI	TTAA	4 cut(s)	225, 378, 813, 1428
MslI	CAYNNNNRTG	2 cut(s)	843, 1570
MspA1I	CMGCKG	1 cut(s)	506
MspI	CCGG	6 cut(s)	23, 94, 147, 183, 942, 1009
MspR9I	CCNGG	4 cut(s)	95, 867, 1010, 1701
MvaI	CCWGG	2 cut(s)	867, 1701
MwoI	GCNNNNNNNGC	5 cut(s)	143, 373, 773, 794, 803
NaeI	GCCGGC	1 cut(s)	24
NciI	CCSGG	2 cut(s)	95, 1010
NcoI	CCATGG	1 cut(s)	1455
NdeI	CATATG	1 cut(s)	1567
NgoMIV	GCCGGC	1 cut(s)	22
NlaIII	CATG	6 cut(s)	1099, 1129, 1165, 1343, 1423, 1459
NlaIV	GGNNCC	3 cut(s)	267, 339, 1577
NmuCI	GTSAC	1 cut(s)	313
NsiI	ATGCAT	1 cut(s)	1199
NspI	RCATGY	1 cut(s)	1129
NspV	TTCGAA	3 cut(s)	106, 160, 1632
PciI	ACATGT	1 cut(s)	1125
PcsI	WCGNNNNNNNCGW	1 cut(s)	413
PdiI	GCCGGC	1 cut(s)	24
PdmI	GAANNNNTTC	1 cut(s)	208
PfeI	GAWTC	6 cut(s)	103, 162, 247, 1320, 1404, 1642
PflMI	CCANNNNNTGG	1 cut(s)	1478
PfoI	TCCNGGA	1 cut(s)	1699
PkrI	GCNGC	8 cut(s)	21, 40, 363, 366, 718, 769, 799, 1453
PleI	GAGTC	5 cut(s)	22, 135, 442, 535, 1181
PpsI	GAGTC	5 cut(s)	22, 135, 442, 535, 1181
PscI	ACATGT	1 cut(s)	1125
Psp124BI	GAGCTC	1 cut(s)	82
Psp1406I	AACGTT	2 cut(s)	206, 381
Psp6I	CCWGG	2 cut(s)	865, 1699
PspGI	CCWGG	2 cut(s)	865, 1699
PspN4I	GGNNCC	3 cut(s)	267, 339, 1577
PspPI	GGNCC	1 cut(s)	998
PstNI	CAGNNNCTG	1 cut(s)	1451
PsuI	RGATCY	5 cut(s)	62, 233, 265, 673, 1081
PvuII	CAGCTG	1 cut(s)	506
RsaI	GTAC	2 cut(s)	358, 1577
RsaNI	GTAC	2 cut(s)	357, 1576
RseI	CAYNNNNRTG	2 cut(s)	843, 1570
SacI	GAGCTC	1 cut(s)	82
SaqAI	TTAA	4 cut(s)	225, 378, 813, 1428
SatI	GCNGC	8 cut(s)	20, 39, 362, 365, 717, 768, 798, 1452
Sau96I	GGNCC	1 cut(s)	998
SchI	GAGTC	5 cut(s)	22, 135, 442, 535, 1181
ScrFI	CCNGG	4 cut(s)	95, 867, 1010, 1701
SduI	GDGCHC	2 cut(s)	82, 976
SfaNI	GCATC	3 cut(s)	403, 1045, 1184
SfuI	TTCGAA	3 cut(s)	106, 160, 1632
SgrAI	CRCCGGYG	1 cut(s)	22
SmiMI	CAYNNNNRTG	2 cut(s)	843, 1570
SmlI	CTYRAG	3 cut(s)	452, 1142, 1268
SmoI	CTYRAG	3 cut(s)	452, 1142, 1268
Sse9I	AATT	9 cut(s)	32, 83, 516, 649, 947, 1046, 1180, 1211, 1295
SsiI	CCGC	6 cut(s)	20, 39, 132, 221, 692, 755
SspMI	CTAG	3 cut(s)	486, 1085, 1580
SstI	GAGCTC	1 cut(s)	82
StyD4I	CCNGG	4 cut(s)	93, 865, 1008, 1699
StyI	CCWWGG	2 cut(s)	903, 1455
TaaI	ACNGT	9 cut(s)	70, 157, 319, 409, 548, 937, 1103, 1447, 1603
TaiI	ACGT	4 cut(s)	209, 384, 1639, 1680
TaqI	TCGA	8 cut(s)	106, 121, 160, 497, 854, 1185, 1323, 1632
TasI	AATT	9 cut(s)	32, 83, 516, 649, 947, 1046, 1180, 1211, 1295
TauI	GCSGC	2 cut(s)	22, 41
TfiI	GAWTC	6 cut(s)	103, 162, 247, 1320, 1404, 1642
Tru1I	TTAA	4 cut(s)	225, 378, 813, 1428
Tru9I	TTAA	4 cut(s)	225, 378, 813, 1428
TscAI	CASTG	3 cut(s)	661, 799, 1608
TseFI	GTSAC	1 cut(s)	313
TseI	GCWGC	6 cut(s)	361, 364, 716, 767, 797, 1451
Tsp45I	GTSAC	1 cut(s)	313
TspDTI	ATGAA	3 cut(s)	464, 1112, 1356
TspGWI	ACGGA	2 cut(s)	258, 1218
TspRI	CASTG	3 cut(s)	661, 799, 1608
Van91I	CCANNNNNTGG	1 cut(s)	1478
XapI	RAATTY	4 cut(s)	516, 1046, 1180, 1295
XbaI	TCTAGA	2 cut(s)	485, 1084
XceI	RCATGY	1 cut(s)	1129
XmiI	GTMKAC	1 cut(s)	989
XmnI	GAANNNNTTC	1 cut(s)	208
XspI	CTAG	3 cut(s)	486, 1085, 1580
Zsp2I	ATGCAT	1 cut(s)	1199

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.