Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0005074

Physical Location & Seq

Genomic Coordinates Forward (+)

23886148 .. 23888817

Gene Structure 2670 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G018490.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1677 bp

ATGGCTGCCGATTCGGCACAGTTAAGTTCCCAACTGGCTGAAGCAACTTTAAACGAATCTGTCTCTGCTCTGAAAGCAGAATTTTCTGACAGAGTTCCCATCAGATCCATAATATCACGAACCGATGGGGGGTCTGGGTTTGCCGGGCAGCATGTCCGGGTTGGTGGTTGGGTGAAGACGGGCAGGAAGGCAGACAAGGATGCATTTGCTTTTCTGGAACTTAATGATGGATCATGCCCTGGCAACTTACAGGTGATTGTGGAAGCAGACAAGGGTGACCTTGGGCAGCTTGTGCCAACCGGTACATGTGTGGTTGTGGATGGCGTGCTTAAGCTGCCCCCAGCTGGGGCCAAGCAGAAGGTGGAGCTTGGAGTTGAGAAGGTGGTCCATTTAGGTCCAGTTGACCCTGCTAAATATCCGTTGCCCAAGACCAAGCTCACCCTTGAAACTAACACCATCTCTGCAGTTGCTCGCATCAGAAATGCCCTGGCATATGCAACTCATACATTTTTCCAAAAGCATGGCTTCCTTTATGTGCACACTCCAATTATCACTACCAGTGATTGTGAGGGTGCTGGTGAGATGTTCCAAGTCACAACATTGATTAGCGAAGGTGAAAGGTTGGAGAGGGAGCTGATTAAGAACCCTCCCCCGTCTGAAGCAGACCTAGAAGCTGCCAAGCTAATCATCAAGGAAAAAGGAGATGCTGTTTCACAGCTGAAATCTGCTAAAGCAAGTAAGGAGGAGATTGGCGCTGCTGTCGCTGAACTTAAAAGGGCGAAGGAGAATGATTTGAAGCTGGAGGAGAGATCCAAGCTTCAGCCAGGAATCCCCAAAAAGGATGGGAAGATTGACTATACACAAGATTTCTTTGCCCGTCAAGCTTTTTTGACCGTTTCTGGCCAACTCCAAGTGGAATCTTATGCATGTGCTCTTAGTAGCGTGTATACATTTGGGCCTACTTTTCGTGCTGAGAATTCACACACTTCACGGCATTTGGCAGAATTCTGGATGGTGGAGCCTGAATTAGCATTTGCAGAGCTCAAGGATGACATGAACTGTGCAGAGGCGTATGTGAAATTCTTGTGTCAGTGGTTACTTGACAACTGCTCTGACGATATGGAGTTTATTTCTCGGCAATTTGATAAAACTTGCATAGACCGTCTAAAGATGGTTGCATCCACACCGTTTGAACGGATTACATATACAGAAGCTGTGGAGCTGCTAATTGATGCTGTGAAAAATGGCAAGAAGTTTGAGAATCATGTAGAGTGGGGGATTGACTTGGCATCTGAACATGAAAGATTCTTAACAGAGGTGAAATTTCAGAAGCCTGTTATTGTGTACAATTACCCAAAAGGGATCAAAGCTTTCTACATGAGACTCAATGATGATAACAAAACAGTGGCTGCTATGGATGTCCTTGTACCAAAGGTGGGAGAGTTGATTGGGGGTAGCCAAAGGGAAGAACGCTATGATGTGATTCATAGCAGGATTGCAGAGATGGGTCTGCCTATTGAGCCGTACGAGTGGTATCTTGACTTGCGCCGCTTTGGAACTGTCAAACATGCCGGTTTTGGTTTAGGGTTTGAGCGGATGCTTTTGTTTGCCACAGGCCTTGACAATATCAGAGATGTTATTCCATTCCCTAGATATCCTGGAAGAGCAGATCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0007275 GO:0008150 GO:0009507 GO:0009536 GO:0009790 GO:0009791 GO:0009793 GO:0010035 GO:0010038 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

WHEP-TRS tRNA-synt_2 tRNA_anti-codon

Protein Analysis

558

Amino Acids

62.0

Weight (kDa)

5.65

Isoelectric Point (pI)

28.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA_anti-codon	PF01336	52 - 128	2.1e-07	OB-fold nucleic acid binding domain
tRNA-synt_2	PF00152	144 - 201	1.1e-06	tRNA synthetases class II (D, K and N)
WHEP-TRS	PF00458	230 - 266	2.1e-06	WHEP-TRS domain
tRNA-synt_2	PF00152	289 - 551	1.2e-50	tRNA synthetases class II (D, K and N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005074)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70980 AT5G56680
fragaria_vesca	FvH4_3g17430
malus_domestica	MD01G1115900.v1.1 MD03G1251800.v1.1 MD05G1158600.v1.1 MD11G1273000.v1.1
prunus_persica	Prupe.4G154500_v2.0.a1 Prupe.4G154500_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1
pyrus_communis	pycom03g19910 pycom05g15060 pycom11g24090
rosa_chinensis	RchiOBHm_Chr5g0028961
rosa_laevigata	RLG00000033131
rosa_multiflora	Rmu_co7984584.1_g000001 Rmu_sc0009042.1_g000005
rosa_roxburghii	Rroxscaffold_1G00050930
rosa_rugosa	Rorug05G0111900 Rorug05G0112000 Rorug05G0112000
rosa_samantha	Rh5AG204000 Rh5BG202300 Rh5CG223100 Rh5DG206100
rosa_wichuraiana	Rw5G018490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	1594
AccI	GTMKAC	1 cut(s)	947
AciI	CCGC	2 cut(s)	1549, 1594
AclWI	GGATC	4 cut(s)	99, 238, 804, 1370
AcoI	YGGCCR	1 cut(s)	901
AcsI	RAATTY	5 cut(s)	80, 976, 1004, 1079, 1322
AcuI	CTGAAG	3 cut(s)	60, 678, 803
AfaI	GTAC	4 cut(s)	304, 1346, 1428, 1526
AfiI	CCNNNNNNNGG	6 cut(s)	129, 344, 345, 346, 838, 1436
AflII	CTTAAG	1 cut(s)	329
AflIII	ACRYGT	1 cut(s)	305
AgeI	ACCGGT	1 cut(s)	299
AgsI	TTSAA	3 cut(s)	446, 796, 1193
AjnI	CCWGG	4 cut(s)	238, 486, 823, 1657
Alw21I	GWGCWC	3 cut(s)	540, 934, 1044
Alw26I	GTCTC	2 cut(s)	67, 1375
Alw44I	GTGCAC	1 cut(s)	536
AlwI	GGATC	4 cut(s)	99, 238, 804, 1370
AlwNI	CAGNNNCTG	2 cut(s)	1214, 1409
AoxI	GGCC	4 cut(s)	348, 901, 956, 1615
ApaLI	GTGCAC	1 cut(s)	536
ApeKI	GCWGC	8 cut(s)	5, 148, 286, 334, 674, 755, 1222, 1409
ApoI	RAATTY	5 cut(s)	80, 976, 1004, 1079, 1322
AsiGI	ACCGGT	1 cut(s)	299
AspLEI	GCGC	2 cut(s)	755, 1548
AspS9I	GGNCC	4 cut(s)	348, 385, 395, 956
AsuC2I	CCSGG	2 cut(s)	145, 158
AsuHPI	GGTGA	7 cut(s)	184, 265, 287, 430, 590, 626, 1330
AvaII	GGWCC	2 cut(s)	385, 395
BaeGI	GKGCMC	1 cut(s)	540
BaeI	ACNNNNGTAYC	2 cut(s)	294, 327
BalI	TGGCCA	1 cut(s)	903
BanII	GRGCYC	1 cut(s)	1044
BbsI	GAAGAC	1 cut(s)	182
Bbv12I	GWGCWC	3 cut(s)	540, 934, 1044
BbvI	GCAGC	7 cut(s)	160, 298, 321, 661, 742, 1209, 1396
BccI	CCATC	9 cut(s)	107, 119, 221, 314, 464, 836, 1006, 1165, 1498
BceAI	ACGGC	2 cut(s)	1007, 1507
BciT130I	CCWGG	4 cut(s)	240, 488, 825, 1659
BcnI	CCSGG	2 cut(s)	145, 158
BcoDI	GTCTC	2 cut(s)	67, 1375
BfaI	CTAG	2 cut(s)	668, 1650
BfmI	CTRYAG	1 cut(s)	462
BfoI	RGCGCY	1 cut(s)	756
BfrI	CTTAAG	1 cut(s)	329
BglI	GCCNNNNNGGC	1 cut(s)	14
BglII	AGATCT	1 cut(s)	1669
BisI	GCNGC	9 cut(s)	6, 149, 287, 335, 675, 756, 1223, 1410, 1549
BlsI	GCNGC	9 cut(s)	7, 150, 288, 336, 676, 757, 1224, 1411, 1550
Bme1390I	CCNGG	6 cut(s)	145, 158, 240, 488, 825, 1659
Bme18I	GGWCC	2 cut(s)	385, 395
BmgT120I	GGNCC	4 cut(s)	348, 385, 395, 956
BmiI	GGNNCC	2 cut(s)	349, 1020
BmrFI	CCNGG	6 cut(s)	145, 158, 240, 488, 825, 1659
BmsI	GCATC	7 cut(s)	190, 483, 694, 1187, 1222, 1298, 1587
BpiI	GAAGAC	1 cut(s)	182
BpmI	CTGGAG	1 cut(s)	821
BpuEI	CTTGAG	1 cut(s)	1028
BpuMI	CCSGG	2 cut(s)	145, 158
BsaJI	CCNNGG	3 cut(s)	238, 280, 486
BsaWI	WCCGGW	1 cut(s)	299
BsaXI	ACNNNNNCTCC	2 cut(s)	693, 723
Bsc4I	CCNNNNNNNGG	6 cut(s)	129, 344, 345, 346, 838, 1436
Bse118I	RCCGGY	2 cut(s)	299, 1571
Bse1I	ACTGG	3 cut(s)	39, 398, 558
BseBI	CCWGG	4 cut(s)	240, 488, 825, 1659
BseDI	CCNNGG	3 cut(s)	238, 280, 486
BseGI	GGATG	8 cut(s)	205, 325, 847, 1017, 1054, 1178, 1423, 1602
BseLI	CCNNNNNNNGG	6 cut(s)	129, 344, 345, 346, 838, 1436
BseMII	CTCAG	1 cut(s)	963
BseNI	ACTGG	3 cut(s)	39, 398, 558
BseRI	GAGGAG	2 cut(s)	758, 818
BseSI	GKGCMC	1 cut(s)	540
BseXI	GCAGC	7 cut(s)	160, 298, 321, 661, 742, 1209, 1396
BseYI	CCCAGC	2 cut(s)	340, 344
BsgI	GTGCAG	1 cut(s)	1083
BshFI	GGCC	4 cut(s)	350, 903, 958, 1617
BshTI	ACCGGT	1 cut(s)	299
BsiHKAI	GWGCWC	3 cut(s)	540, 934, 1044
BsiSI	CCGG	4 cut(s)	144, 157, 300, 1572
BsiWI	CGTACG	1 cut(s)	1524
BslI	CCNNNNNNNGG	6 cut(s)	129, 344, 345, 346, 838, 1436
BsmAI	GTCTC	2 cut(s)	67, 1375
BsnI	GGCC	4 cut(s)	350, 903, 958, 1617
Bsp1286I	GDGCHC	3 cut(s)	540, 934, 1044
Bsp1407I	TGTACA	1 cut(s)	1344
Bsp143I	GATC	5 cut(s)	104, 230, 809, 1362, 1669
BspACI	CCGC	2 cut(s)	1549, 1594
BspANI	GGCC	4 cut(s)	350, 903, 958, 1617
BspCNI	CTCAG	1 cut(s)	964
BspLI	GGNNCC	2 cut(s)	349, 1020
BspMAI	CTGCAG	1 cut(s)	466
BspPI	GGATC	4 cut(s)	99, 238, 804, 1370
BspQI	GCTCTTC	1 cut(s)	1657
BspTI	CTTAAG	1 cut(s)	329
BsrBI	CCGCTC	1 cut(s)	1594
BsrFI	RCCGGY	2 cut(s)	299, 1571
BsrGI	TGTACA	1 cut(s)	1344
BsrI	ACTGG	3 cut(s)	39, 398, 558
BssAI	RCCGGY	2 cut(s)	299, 1571
BssECI	CCNNGG	3 cut(s)	238, 280, 486
BssMI	GATC	5 cut(s)	104, 230, 809, 1362, 1669
BssNAI	GTATAC	1 cut(s)	948
BssT1I	CCWWGG	1 cut(s)	280
Bst1107I	GTATAC	1 cut(s)	948
Bst2UI	CCWGG	4 cut(s)	240, 488, 825, 1659
Bst4CI	ACNGT	7 cut(s)	21, 895, 1061, 1163, 1188, 1405, 1561
Bst6I	CTCTTC	1 cut(s)	1657
BstAFI	CTTAAG	1 cut(s)	329
BstAPI	GCANNNNNTGC	1 cut(s)	292
BstAUI	TGTACA	1 cut(s)	1344
BstC8I	GCNNGC	2 cut(s)	326, 472
BstDEI	CTNAG	2 cut(s)	935, 972
BstEII	GGTNACC	1 cut(s)	275
BstF5I	GGATG	8 cut(s)	205, 325, 847, 1017, 1054, 1178, 1423, 1602
BstH2I	RGCGCY	1 cut(s)	756
BstHHI	GCGC	2 cut(s)	755, 1548
BstKTI	GATC	5 cut(s)	107, 233, 812, 1365, 1672
BstMAI	GTCTC	2 cut(s)	67, 1375
BstMBI	GATC	5 cut(s)	104, 230, 809, 1362, 1669
BstMWI	GCNNNNNNNGC	7 cut(s)	14, 74, 292, 334, 761, 881, 1519
BstNI	CCWGG	4 cut(s)	240, 488, 825, 1659
BstNSI	RCATGY	4 cut(s)	155, 309, 930, 1571
BstPI	GGTNACC	1 cut(s)	275
BstSCI	CCNGG	6 cut(s)	143, 156, 238, 486, 823, 1657
BstSFI	CTRYAG	1 cut(s)	462
BstSLI	GKGCMC	1 cut(s)	540
BstV1I	GCAGC	7 cut(s)	160, 298, 321, 661, 742, 1209, 1396
BstV2I	GAAGAC	1 cut(s)	182
BstX2I	RGATCY	3 cut(s)	104, 809, 1669
BstXI	CCANNNNNNTGG	1 cut(s)	521
BstYI	RGATCY	3 cut(s)	104, 809, 1669
BstZ17I	GTATAC	1 cut(s)	948
BsuRI	GGCC	4 cut(s)	350, 903, 958, 1617
BtsCI	GGATG	8 cut(s)	205, 325, 847, 1017, 1054, 1178, 1423, 1602
BtsIMutI	CAGTG	3 cut(s)	565, 1097, 1410
Cac8I	GCNNGC	2 cut(s)	326, 472
CaiI	CAGNNNCTG	2 cut(s)	1214, 1409
CfoI	GCGC	2 cut(s)	755, 1548
Cfr10I	RCCGGY	2 cut(s)	299, 1571
Cfr13I	GGNCC	4 cut(s)	348, 385, 395, 956
Csp6I	GTAC	4 cut(s)	303, 1345, 1427, 1525
CspAI	ACCGGT	1 cut(s)	299
CspCI	CAANNNNNGTGG	4 cut(s)	1171, 1206, 1600, 1635
CviQI	GTAC	4 cut(s)	303, 1345, 1427, 1525
DdeI	CTNAG	2 cut(s)	935, 972
DpnI	GATC	5 cut(s)	106, 232, 811, 1364, 1671
DpnII	GATC	5 cut(s)	104, 230, 809, 1362, 1669
DraI	TTTAAA	1 cut(s)	51
EaeI	YGGCCR	1 cut(s)	901
Eam1104I	CTCTTC	1 cut(s)	1657
EarI	CTCTTC	1 cut(s)	1657
Ecl136II	GAGCTC	1 cut(s)	1042
Eco130I	CCWWGG	1 cut(s)	280
Eco147I	AGGCCT	1 cut(s)	1617
Eco24I	GRGCYC	1 cut(s)	1044
Eco32I	GATATC	1 cut(s)	1655
Eco47I	GGWCC	2 cut(s)	385, 395
Eco53kI	GAGCTC	1 cut(s)	1042
Eco57I	CTGAAG	3 cut(s)	60, 678, 803
Eco91I	GGTNACC	1 cut(s)	275
EcoICRI	GAGCTC	1 cut(s)	1042
EcoO65I	GGTNACC	1 cut(s)	275
EcoRI	GAATTC	2 cut(s)	976, 1004
EcoRII	CCWGG	4 cut(s)	238, 486, 823, 1657
EcoRV	GATATC	1 cut(s)	1655
EcoT14I	CCWWGG	1 cut(s)	280
EcoT22I	ATGCAT	2 cut(s)	205, 928
EcoT38I	GRGCYC	1 cut(s)	1044
ErhI	CCWWGG	1 cut(s)	280
FalI	AAGNNNNNCTT	2 cut(s)	509, 541
FauNDI	CATATG	1 cut(s)	493
FblI	GTMKAC	1 cut(s)	947
Fnu4HI	GCNGC	9 cut(s)	6, 149, 287, 335, 675, 756, 1223, 1410, 1549
FokI	GGATG	8 cut(s)	212, 332, 854, 1024, 1061, 1165, 1430, 1609
FriOI	GRGCYC	1 cut(s)	1044
Fsp4HI	GCNGC	9 cut(s)	6, 149, 287, 335, 675, 756, 1223, 1410, 1549
FspBI	CTAG	2 cut(s)	668, 1650
GlaI	GCGC	2 cut(s)	754, 1547
GluI	GCNGC	9 cut(s)	6, 149, 287, 335, 675, 756, 1223, 1410, 1549
GsaI	CCCAGC	2 cut(s)	344, 348
GsuI	CTGGAG	1 cut(s)	821
HaeII	RGCGCY	1 cut(s)	756
HaeIII	GGCC	4 cut(s)	350, 903, 958, 1617
HapII	CCGG	4 cut(s)	144, 157, 300, 1572
HhaI	GCGC	2 cut(s)	755, 1548
Hin6I	GCGC	2 cut(s)	753, 1546
HinP1I	GCGC	2 cut(s)	753, 1546
HincII	GTYRAC	1 cut(s)	403
HindII	GTYRAC	1 cut(s)	403
HindIII	AAGCTT	3 cut(s)	815, 882, 1368
HinfI	GANTC	8 cut(s)	11, 56, 828, 917, 1261, 1305, 1383, 1483
HpaII	CCGG	4 cut(s)	144, 157, 300, 1572
HphI	GGTGA	7 cut(s)	184, 265, 287, 430, 590, 626, 1330
Hpy166II	GTNNAC	4 cut(s)	403, 538, 948, 1345
Hpy188I	TCNGA	9 cut(s)	72, 88, 104, 479, 658, 1114, 1294, 1329, 1631
Hpy188III	TCNNGA	4 cut(s)	117, 215, 1009, 1538
Hpy8I	GTNNAC	4 cut(s)	403, 538, 948, 1345
HpyAV	CCTTC	5 cut(s)	181, 352, 373, 605, 775
HpyCH4III	ACNGT	7 cut(s)	21, 895, 1061, 1163, 1188, 1405, 1561
HpyF10VI	GCNNNNNNNGC	7 cut(s)	14, 74, 292, 334, 761, 881, 1519
HpyF3I	CTNAG	2 cut(s)	935, 972
HspAI	GCGC	2 cut(s)	753, 1546
Kzo9I	GATC	5 cut(s)	104, 230, 809, 1362, 1669
LguI	GCTCTTC	1 cut(s)	1657
LmnI	GCTCC	4 cut(s)	364, 631, 1018, 1219
Lsp1109I	GCAGC	7 cut(s)	160, 298, 321, 661, 742, 1209, 1396
LweI	GCATC	7 cut(s)	190, 483, 694, 1187, 1222, 1298, 1587
MaeI	CTAG	2 cut(s)	668, 1650
MaeIII	GTNAC	3 cut(s)	275, 592, 1095
MalI	GATC	5 cut(s)	106, 232, 811, 1364, 1671
MbiI	CCGCTC	1 cut(s)	1594
MboI	GATC	5 cut(s)	104, 230, 809, 1362, 1669
MboII	GAAGA	4 cut(s)	187, 859, 1478, 1674
MflI	RGATCY	3 cut(s)	104, 809, 1669
MhlI	GDGCHC	3 cut(s)	540, 934, 1044
MlsI	TGGCCA	1 cut(s)	903
MluNI	TGGCCA	1 cut(s)	903
MlyI	GAGTC	1 cut(s)	1377
MmeI	TCCRAC	1 cut(s)	603
MnlI	CCTC	7 cut(s)	562, 621, 657, 736, 796, 1060, 1309
Mox20I	TGGCCA	1 cut(s)	903
Mph1103I	ATGCAT	2 cut(s)	205, 928
MscI	TGGCCA	1 cut(s)	903
MseI	TTAA	8 cut(s)	23, 50, 222, 330, 639, 771, 1310, 1675
Msp20I	TGGCCA	1 cut(s)	903
MspA1I	CMGCKG	2 cut(s)	344, 718
MspCI	CTTAAG	1 cut(s)	329
MspI	CCGG	4 cut(s)	144, 157, 300, 1572
MspR9I	CCNGG	6 cut(s)	145, 158, 240, 488, 825, 1659
MvaI	CCWGG	4 cut(s)	240, 488, 825, 1659
MwoI	GCNNNNNNNGC	7 cut(s)	14, 74, 292, 334, 761, 881, 1519
NciI	CCSGG	2 cut(s)	145, 158
NdeI	CATATG	1 cut(s)	493
NdeII	GATC	5 cut(s)	104, 230, 809, 1362, 1669
NlaIV	GGNNCC	2 cut(s)	349, 1020
NmeAIII	GCCGAG	1 cut(s)	1114
NmuCI	GTSAC	2 cut(s)	275, 592
NsiI	ATGCAT	2 cut(s)	205, 928
NspI	RCATGY	4 cut(s)	155, 309, 930, 1571
PceI	AGGCCT	1 cut(s)	1617
PciI	ACATGT	1 cut(s)	305
PciSI	GCTCTTC	1 cut(s)	1657
PfeI	GAWTC	7 cut(s)	11, 56, 828, 917, 1261, 1305, 1483
Pfl23II	CGTACG	1 cut(s)	1524
PfoI	TCCNGGA	1 cut(s)	1657
PinAI	ACCGGT	1 cut(s)	299
PkrI	GCNGC	9 cut(s)	7, 150, 288, 336, 676, 757, 1224, 1411, 1550
PleI	GAGTC	1 cut(s)	1377
PpsI	GAGTC	1 cut(s)	1377
PscI	ACATGT	1 cut(s)	305
Psp124BI	GAGCTC	1 cut(s)	1044
Psp6I	CCWGG	4 cut(s)	238, 486, 823, 1657
PspEI	GGTNACC	1 cut(s)	275
PspFI	CCCAGC	2 cut(s)	340, 344
PspGI	CCWGG	4 cut(s)	238, 486, 823, 1657
PspLI	CGTACG	1 cut(s)	1524
PspN4I	GGNNCC	2 cut(s)	349, 1020
PspPI	GGNCC	4 cut(s)	348, 385, 395, 956
PstI	CTGCAG	1 cut(s)	466
PstNI	CAGNNNCTG	2 cut(s)	1214, 1409
PsuI	RGATCY	3 cut(s)	104, 809, 1669
PvuII	CAGCTG	2 cut(s)	344, 718
RsaI	GTAC	4 cut(s)	304, 1346, 1428, 1526
RsaNI	GTAC	4 cut(s)	303, 1345, 1427, 1525
SacI	GAGCTC	1 cut(s)	1044
SapI	GCTCTTC	1 cut(s)	1657
SaqAI	TTAA	8 cut(s)	23, 50, 222, 330, 639, 771, 1310, 1675
SatI	GCNGC	9 cut(s)	6, 149, 287, 335, 675, 756, 1223, 1410, 1549
Sau3AI	GATC	5 cut(s)	104, 230, 809, 1362, 1669
Sau96I	GGNCC	4 cut(s)	348, 385, 395, 956
SchI	GAGTC	1 cut(s)	1377
ScrFI	CCNGG	6 cut(s)	145, 158, 240, 488, 825, 1659
SduI	GDGCHC	3 cut(s)	540, 934, 1044
SfaNI	GCATC	7 cut(s)	190, 483, 694, 1187, 1222, 1298, 1587
SfcI	CTRYAG	1 cut(s)	462
SinI	GGWCC	2 cut(s)	385, 395
SmlI	CTYRAG	2 cut(s)	329, 1043
SmoI	CTYRAG	2 cut(s)	329, 1043
SseBI	AGGCCT	1 cut(s)	1617
SsiI	CCGC	2 cut(s)	1549, 1594
SspMI	CTAG	2 cut(s)	668, 1650
SstI	GAGCTC	1 cut(s)	1044
StuI	AGGCCT	1 cut(s)	1617
StyD4I	CCNGG	6 cut(s)	143, 156, 238, 486, 823, 1657
StyI	CCWWGG	1 cut(s)	280
TaaI	ACNGT	7 cut(s)	21, 895, 1061, 1163, 1188, 1405, 1561
TatI	WGTACW	1 cut(s)	1344
TauI	GCSGC	1 cut(s)	1551
TfiI	GAWTC	7 cut(s)	11, 56, 828, 917, 1261, 1305, 1483
Tru1I	TTAA	8 cut(s)	23, 50, 222, 330, 639, 771, 1310, 1675
Tru9I	TTAA	8 cut(s)	23, 50, 222, 330, 639, 771, 1310, 1675
TscAI	CASTG	3 cut(s)	565, 1097, 1410
TseFI	GTSAC	2 cut(s)	275, 592
TseI	GCWGC	8 cut(s)	5, 148, 286, 334, 674, 755, 1222, 1409
Tsp45I	GTSAC	2 cut(s)	275, 592
TspDTI	ATGAA	3 cut(s)	1070, 1314, 1475
TspGWI	ACGGA	2 cut(s)	408, 1210
TspRI	CASTG	3 cut(s)	565, 1097, 1410
Vha464I	CTTAAG	1 cut(s)	329
VneI	GTGCAC	1 cut(s)	536
VpaK11BI	GGWCC	2 cut(s)	385, 395
XapI	RAATTY	5 cut(s)	80, 976, 1004, 1079, 1322
XceI	RCATGY	4 cut(s)	155, 309, 930, 1571
XcmI	CCANNNNNNNNNTGG	1 cut(s)	358
XmiI	GTMKAC	1 cut(s)	947
XspI	CTAG	2 cut(s)	668, 1650
Zsp2I	ATGCAT	2 cut(s)	205, 928

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.