Rorug05G0111900

asparagine--tRNA ligase, cytoplasmic 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005074
Physical Location & Seq
Reverse (-)
9968036 .. 9972290
4255 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0111900.1

Sequence Viewer

Length: 2472 bp
ATGGTCTCTATTCGTAGCTACATTCGAACCGGAGGAGCAGCTCGGCTCAGACCTCATAACTCGTGTGAGTCCACCACATCTTTCTCTCACTACCCTTTAACTTTTGTTGCGCTCCCTCAATTTTTCTCATCAGTTAGCTCAACAAACTCAAGTTCAGATTTTTGCTCTGGAATTAGACCTCATTCTGTTGAAAGTTATGATTTTGATGATTCTAATAACGGGAAAGGTAGTTTTAGGAAAATGGGTAATCGAAATACGGCGGGTTTGAGTTTCTTAGGAGTTGCCCAGAGTGACTGTGAAAACCCCCAAAGTGAAAGTTTCAAACTTTTTGAAGTTTCAGATAGCGGAGAAGAGAATAATAGTGATGGTGATGATGAGTTAATGATGTTGAATTCTAATAGGGGTTGTGAGCAAAAAGAGAATCTGGGTAGAGTTGAAGGAGGTGGGGATGAGGATGAATATAGACACCCTTTGGTGAGAGAGGTTTGCAGGTTAATTGAGCTTAGAACAGCTTGGACCCCAAAGTTTGAAGGTGAGTTAAGGCACTTGTTCCGAAGCCTGAAGCCGAAGCAGGTCTGTGCCGTTTTGAAGTCTCAGGCTGATGAAAGAGTTGCTTTGAAGATTTTTTACTGGGCTGATAGGCAGTGGCGGTATAAGCATGACCCGATTGTGTACTATGCAATGCTTGAGGTGCTTGGCAAGACTAAGTTGTGCCAAGGTGCTAGGAGGGTTCTTAGGTTAATGGCCCGTAGGCGGATTGAGCTTCGTTCTGAAGCTTTTGGCCATGTGATGGTGTCATATAGTCGAGCGGGCAAGTTGAGGCACGCGTTGCGTGTGTTGACTTTGATGCAGAAAGCGGGGCTCGAGCTTGATTTGTCAATGTGTAATACTGCAATTTATGTTTTGGTTAAGGGTAATAAGTTGGAGAAGGCGTTGAGGACCTTGGAAAGGATGAAGCTTGTTGGGATTGCACCAAATGTAGTCACTTATAACTGTTTGATAAAGGGGTATTGTGATCTGCATCGTGTAGAAGATGCAATCCAGGTGATTGATGAAATGCCGAGGAAGGGGTGTGCTCCTGATAAAGTTAGTTACTATACCGTGATGGATTTCCTTTCTAAGGAGAAGAGGGTCAAAGAAGTGAGGGACTTGATGGAGAAGATGATCAAGGATGGTGGGTTGTTACCAGACCAGGTCACTTATAATAATCTTGTCCATGTGCTTTGTAAGCATGGCTATGGTGATGAGGCACTTGAATTTTTAAGGGAAGCCGAAGAGAAGGGATTTCGGTTTGATAAGGTTGGGTATAGTGCAATTGTACATTCATTTTGTAAGGGTGGTCGGACTGACATGGCAAAAGAAATTGTTAGTGAAATGTTCTCAAAAGGTTGCATCCCAGATGTTGTAACATACACTGCTGTTCTGAATGGGTATTGTCGACTTGGGAAGTTGGATCAAGCTAAAAAGATGCTTCAGCACATGCATAAGCATGGTTGCAAGCCAAATACTGTATCATATACGGCCTTATTAAATGGGCTTTGTCGTGGTGGGAATTCATTGGAGGCAAGAGAGATGATGAACATGAGTGAGGAAGAATGGTGGACACCAAATGCCATCACTTATAGTGTTCTGATGCATGGGTTCCGGAGGGAAGGGAAATTGGTTGAGGCTTGTGATACTGTCAAAGAGATGATTAAAAAGGGATTTTTCCCTACCCCAGTTGAAATTAATCTCTTAATTCAGTCTCTTTGCCAAGAGGGGAAGATGGATGAGGCTAAACGTTTCATGGAGGAGTGTCTAAATAAAGGTTGTGCTGTTAATGTTGTCAATTTTACTACTGTTATTCATGGATATTGTCAAAAAGATGACTTGGAAGCTGCTCTGTCGTTGCTTGATGACATGTATCTCAGCAATAAACACCCAGATGCAGTAACTTATACAGCAATGATCAGTGCATTGGGAAAGAAAGGTAGAATACAAGACGCAACAGAACTTATGATGAAGATGCTGAATAAGGGTATTGACCCGACTCCCGTCACATACAGGACGATCATCCATTGGTATTGTAAGATGAGTAGGGTGGATGATTTGTTGAAATTATTGGAGAAGATGTTCTTAAGGCAAAATTGCAAAACAGCATATAATCAAGTGATTGAGAAGCTTTGTAGTTTTGGGAACTTCGAGGACGCTGATAAACTATTGGGTAAGGTGTTGAGAACAGCTTCAAGAGTTGATGCTAAAACATGCCATGTTGTTATGGATGGTTATTTGAGAAAAGGGATTCCTCTGTCAGCATACAAAGTGGCTTGTCGAATGTTCAATCGGAATCTGATTCCTGATTTAAAGTTGTGTGAGAAGGTGACGAAGAGACTGATGCTAGAAGGAAACTCCAAAGAGGCAGACAACCTTATGTTACGGTTTGTTGAGCGTGGATGCATATCAAACCAACATCAGGAACATCTGGAAAGCTAA

Protein Analysis

823

Amino Acids

93.6

Weight (kDa)

8.66

Isoelectric Point (pI)

33.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 229 - 319 4.5e-07 Fungal tetratrico peptide repeats
PPR_long PF17177 260 - 423 2e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 279 - 337 5e-12 Pentatricopeptide repeat domain
PPR_2 PF13041 294 - 339 3.2e-11 PPR repeat family
PPR_1 PF12854 322 - 353 1.2e-11 PPR repeat
PPR_2 PF13041 325 - 369 3.4e-15 PPR repeat family
PPR PF01535 328 - 358 1.1e-07 PPR repeat
PPR_long PF17177 373 - 551 3.3e-12 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 393 - 423 1.7e-08 PPR repeat
PPR_2 PF13041 396 - 445 5.8e-10 PPR repeat family
PPR PF01535 399 - 429 1.5e-06 PPR repeat
PPR_3 PF13812 421 - 475 4.7e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 433 - 480 2.9e-12 PPR repeat family
PPR_3 PF13812 455 - 511 4.6e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 462 - 494 1e-11 PPR repeat
PPR_2 PF13041 466 - 515 4.4e-19 PPR repeat family
PPR PF01535 469 - 499 8.2e-08 PPR repeat
PPR_1 PF12854 497 - 527 9.7e-08 PPR repeat
PPR_1 PF12854 602 - 634 4.4e-08 PPR repeat
PPR_2 PF13041 607 - 655 1.9e-13 PPR repeat family
PPR_3 PF13812 629 - 690 2.8e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 640 - 669 5.5e-07 PPR repeat
PPR_2 PF13041 641 - 690 3e-14 PPR repeat family
PPR PF01535 644 - 674 6.1e-07 PPR repeat
PPR_1 PF12854 672 - 704 8.3e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 990, 1203
Acc36I ACCTGC 2 cut(s) 480, 562
AccB7I CCANNNNNTGG 1 cut(s) 790
AccBSI CCGCTC 1 cut(s) 809
AccI GTMKAC 1 cut(s) 1438
AccII CGCG 1 cut(s) 827
AccIII TCCGGA 1 cut(s) 1644
AciI CCGC 6 cut(s) 260, 345, 649, 754, 809, 857
AclI AACGTT 1 cut(s) 1780
AclWI GGATC 1 cut(s) 1461
AcoI YGGCCR 1 cut(s) 781
AcsI RAATTY 3 cut(s) 391, 1256, 1552
AcuI CTGAAG 3 cut(s) 581, 792, 1457
AfaI GTAC 2 cut(s) 674, 1320
AfiI CCNNNNNNNGG 6 cut(s) 753, 790, 948, 1067, 1120, 2452
AflII CTTAAG 1 cut(s) 2116
AflIII ACRYGT 2 cut(s) 825, 1899
AjnI CCWGG 2 cut(s) 1041, 1191
AloI GAACNNNNNNTCC 2 cut(s) 2096, 2128
Alw21I GWGCWC 1 cut(s) 1078
Alw26I GTCTC 4 cut(s) 10, 597, 1749, 2362
AlwI GGATC 1 cut(s) 1461
Ama87I CYCGRG 1 cut(s) 863
Aor13HI TCCGGA 1 cut(s) 1644
AoxI GGCC 3 cut(s) 744, 781, 1521
ApeKI GCWGC 2 cut(s) 38, 1877
ApoI RAATTY 3 cut(s) 391, 1256, 1552
AseI ATTAAT 1 cut(s) 1728
Asp700I GAANNNNTTC 2 cut(s) 2111, 2221
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 3 cut(s) 516, 745, 939
AsuHPI GGTGA 6 cut(s) 380, 487, 545, 1057, 1253, 2371
AsuII TTCGAA 1 cut(s) 25
AvaI CYCGRG 1 cut(s) 863
AvaII GGWCC 2 cut(s) 516, 939
BalI TGGCCA 1 cut(s) 783
BanII GRGCYC 1 cut(s) 864
BarI GAAGNNNNNNTAC 2 cut(s) 611, 643
BauI CACGAG 1 cut(s) 61
Bbv12I GWGCWC 1 cut(s) 1078
BbvI GCAGC 2 cut(s) 50, 1864
BccI CCATC 8 cut(s) 359, 784, 1099, 1147, 1166, 1622, 1759, 2255
BceAI ACGGC 3 cut(s) 273, 566, 1536
BciT130I CCWGG 2 cut(s) 1043, 1193
BclI TGATCA 2 cut(s) 1164, 1947
BcoDI GTCTC 4 cut(s) 10, 597, 1749, 2362
BfaI CTAG 2 cut(s) 723, 2378
BfrI CTTAAG 1 cut(s) 2116
BfuAI ACCTGC 2 cut(s) 480, 562
BisI GCNGC 2 cut(s) 39, 1878
BlsI GCNGC 2 cut(s) 40, 1879
Bme1390I CCNGG 2 cut(s) 1043, 1193
Bme18I GGWCC 2 cut(s) 516, 939
BmeT110I CYCGRG 1 cut(s) 863
BmgT120I GGNCC 3 cut(s) 516, 745, 939
BmiI GGNNCC 2 cut(s) 518, 1643
BmrFI CCNGG 2 cut(s) 1043, 1193
BmrI ACTGGG 2 cut(s) 640, 1712
BmuI ACTGGG 2 cut(s) 640, 1712
BoxI GACNNNNGTC 1 cut(s) 2033
Bpu14I TTCGAA 1 cut(s) 25
BpuEI CTTGAG 2 cut(s) 133, 707
BsaBI GATNNNNATC 2 cut(s) 1020, 2437
BsaI GGTCTC 1 cut(s) 10
BsaJI CCNNGG 3 cut(s) 715, 942, 1061
BsaWI WCCGGW 2 cut(s) 29, 1644
BsaXI ACNNNNNCTCC 4 cut(s) 917, 947, 2096, 2126
Bsc4I CCNNNNNNNGG 6 cut(s) 753, 790, 948, 1067, 1120, 2452
Bse1I ACTGG 2 cut(s) 635, 1718
Bse3DI GCAATG 2 cut(s) 687, 1950
Bse8I GATNNNNATC 2 cut(s) 1020, 2437
BseAI TCCGGA 1 cut(s) 1644
BseBI CCWGG 2 cut(s) 1043, 1193
BseDI CCNNGG 3 cut(s) 715, 942, 1061
BseJI GATNNNNATC 2 cut(s) 1020, 2437
BseLI CCNNNNNNNGG 6 cut(s) 753, 790, 948, 1067, 1120, 2452
BseMI GCAATG 2 cut(s) 687, 1950
BseMII CTCAG 3 cut(s) 61, 608, 1921
BseNI ACTGG 2 cut(s) 635, 1718
BseRI GAGGAG 2 cut(s) 48, 1805
BseXI GCAGC 2 cut(s) 50, 1864
Bsh1236I CGCG 1 cut(s) 827
BshFI GGCC 3 cut(s) 746, 783, 1523
BsiHKAI GWGCWC 1 cut(s) 1078
BsiHKCI CYCGRG 1 cut(s) 863
BsiSI CCGG 2 cut(s) 30, 1645
BslFI GGGAC 1 cut(s) 1160
BslI CCNNNNNNNGG 6 cut(s) 753, 790, 948, 1067, 1120, 2452
BsmAI GTCTC 4 cut(s) 10, 597, 1749, 2362
BsmFI GGGAC 1 cut(s) 1160
BsnI GGCC 3 cut(s) 746, 783, 1523
Bso31I GGTCTC 1 cut(s) 10
BsoBI CYCGRG 1 cut(s) 863
Bsp119I TTCGAA 1 cut(s) 25
Bsp1286I GDGCHC 2 cut(s) 864, 1078
Bsp13I TCCGGA 1 cut(s) 1644
Bsp1407I TGTACA 1 cut(s) 1318
Bsp143I GATC 5 cut(s) 1015, 1164, 1453, 1947, 2049
BspACI CCGC 6 cut(s) 260, 345, 649, 754, 809, 857
BspANI GGCC 3 cut(s) 746, 783, 1523
BspCNI CTCAG 3 cut(s) 60, 607, 1920
BspEI TCCGGA 1 cut(s) 1644
BspFNI CGCG 1 cut(s) 827
BspLI GGNNCC 2 cut(s) 518, 1643
BspMI ACCTGC 2 cut(s) 480, 562
BspPI GGATC 1 cut(s) 1461
BspT104I TTCGAA 1 cut(s) 25
BspTI CTTAAG 1 cut(s) 2116
BspTNI GGTCTC 1 cut(s) 10
BsrBI CCGCTC 1 cut(s) 809
BsrDI GCAATG 2 cut(s) 687, 1950
BsrGI TGTACA 1 cut(s) 1318
BsrI ACTGG 2 cut(s) 635, 1718
BssECI CCNNGG 3 cut(s) 715, 942, 1061
BssMI GATC 5 cut(s) 1015, 1164, 1453, 1947, 2049
BssSI CACGAG 1 cut(s) 61
BssT1I CCWWGG 2 cut(s) 715, 942
Bst2BI CACGAG 1 cut(s) 61
Bst2UI CCWGG 2 cut(s) 1043, 1193
Bst4CI ACNGT 7 cut(s) 296, 995, 1102, 1510, 1681, 1840, 2418
Bst6I CTCTTC 4 cut(s) 345, 1121, 1269, 2360
BstAFI CTTAAG 1 cut(s) 2116
BstAPI GCANNNNNTGC 1 cut(s) 829
BstAUI TGTACA 1 cut(s) 1318
BstBI TTCGAA 1 cut(s) 25
BstC8I GCNNGC 3 cut(s) 811, 825, 1499
BstDEI CTNAG 8 cut(s) 47, 274, 503, 594, 705, 734, 1119, 1907
BstENI CCTNNNNNAGG 2 cut(s) 946, 1118
BstFNI CGCG 1 cut(s) 827
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 5 cut(s) 1018, 1167, 1456, 1950, 2052
BstMAI GTCTC 4 cut(s) 10, 597, 1749, 2362
BstMBI GATC 5 cut(s) 1015, 1164, 1453, 1947, 2049
BstMWI GCNNNNNNNGC 5 cut(s) 655, 691, 760, 829, 1228
BstNI CCWGG 2 cut(s) 1043, 1193
BstNSI RCATGY 3 cut(s) 1483, 1903, 2247
BstPAI GACNNNNGTC 1 cut(s) 2033
BstSCI CCNGG 2 cut(s) 1041, 1191
BstUI CGCG 1 cut(s) 827
BstV1I GCAGC 2 cut(s) 50, 1864
BsuRI GGCC 3 cut(s) 746, 783, 1523
BtsI GCAGTG 2 cut(s) 650, 1413
BtsIMutI CAGTG 3 cut(s) 650, 1413, 1957
BveI ACCTGC 2 cut(s) 480, 562
Cac8I GCNNGC 3 cut(s) 811, 825, 1499
CfoI GCGC 1 cut(s) 112
Cfr13I GGNCC 3 cut(s) 516, 745, 939
CseI GACGC 2 cut(s) 1991, 2195
CsiI ACCWGGT 1 cut(s) 1191
Csp6I GTAC 2 cut(s) 673, 1319
CspCI CAANNNNNGTGG 2 cut(s) 1156, 1191
CviQI GTAC 2 cut(s) 673, 1319
DdeI CTNAG 8 cut(s) 47, 274, 503, 594, 705, 734, 1119, 1907
DpnI GATC 5 cut(s) 1017, 1166, 1455, 1949, 2051
DpnII GATC 5 cut(s) 1015, 1164, 1453, 1947, 2049
DraI TTTAAA 1 cut(s) 2343
EaeI YGGCCR 1 cut(s) 781
Eam1104I CTCTTC 4 cut(s) 345, 1121, 1269, 2360
EarI CTCTTC 4 cut(s) 345, 1121, 1269, 2360
EciI GGCGGA 1 cut(s) 769
Eco130I CCWWGG 2 cut(s) 715, 942
Eco24I GRGCYC 1 cut(s) 864
Eco31I GGTCTC 1 cut(s) 10
Eco47I GGWCC 2 cut(s) 516, 939
Eco57I CTGAAG 3 cut(s) 581, 792, 1457
Eco88I CYCGRG 1 cut(s) 863
EcoNI CCTNNNNNAGG 2 cut(s) 946, 1118
EcoO109I RGGNCCY 1 cut(s) 939
EcoRI GAATTC 2 cut(s) 391, 1552
EcoRII CCWGG 2 cut(s) 1041, 1191
EcoT14I CCWWGG 2 cut(s) 715, 942
EcoT22I ATGCAT 3 cut(s) 1485, 1638, 2438
EcoT38I GRGCYC 1 cut(s) 864
ErhI CCWWGG 2 cut(s) 715, 942
FalI AAGNNNNNCTT 4 cut(s) 598, 630, 2099, 2131
FaqI GGGAC 1 cut(s) 1160
FauI CCCGC 3 cut(s) 253, 802, 850
FbaI TGATCA 2 cut(s) 1164, 1947
FblI GTMKAC 1 cut(s) 1438
Fnu4HI GCNGC 2 cut(s) 39, 1878
FriOI GRGCYC 1 cut(s) 864
Fsp4HI GCNGC 2 cut(s) 39, 1878
FspBI CTAG 2 cut(s) 723, 2378
GlaI GCGC 1 cut(s) 111
GluI GCNGC 2 cut(s) 39, 1878
HaeIII GGCC 3 cut(s) 746, 783, 1523
HapII CCGG 2 cut(s) 30, 1645
HgaI GACGC 2 cut(s) 1991, 2195
HhaI GCGC 1 cut(s) 112
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HincII GTYRAC 2 cut(s) 840, 1439
HindII GTYRAC 2 cut(s) 840, 1439
HindIII AAGCTT 3 cut(s) 774, 956, 2159
HinfI GANTC 7 cut(s) 68, 209, 421, 2029, 2281, 2326, 2332
HpaII CCGG 2 cut(s) 30, 1645
HphI GGTGA 6 cut(s) 380, 487, 545, 1057, 1253, 2371
Hpy166II GTNNAC 5 cut(s) 72, 673, 840, 1439, 1602
Hpy188III TCNNGA 7 cut(s) 168, 1079, 1645, 2226, 2336, 2453, 2462
Hpy8I GTNNAC 5 cut(s) 72, 673, 840, 1439, 1602
HpyAV CCTTC 8 cut(s) 431, 524, 922, 1060, 1273, 1646, 2350, 2375
HpyCH4III ACNGT 7 cut(s) 296, 995, 1102, 1510, 1681, 1840, 2418
HpyCH4IV ACGT 1 cut(s) 1780
HpyF10VI GCNNNNNNNGC 5 cut(s) 655, 691, 760, 829, 1228
HpyF3I CTNAG 8 cut(s) 47, 274, 503, 594, 705, 734, 1119, 1907
HpySE526I ACGT 1 cut(s) 1780
HspAI GCGC 1 cut(s) 110
Kpn2I TCCGGA 1 cut(s) 1644
Ksp22I TGATCA 2 cut(s) 1164, 1947
Kzo9I GATC 5 cut(s) 1015, 1164, 1453, 1947, 2049
LmnI GCTCC 3 cut(s) 35, 117, 1081
Lsp1109I GCAGC 2 cut(s) 50, 1864
MabI ACCWGGT 1 cut(s) 1191
MaeI CTAG 2 cut(s) 723, 2378
MaeII ACGT 1 cut(s) 1780
MalI GATC 5 cut(s) 1017, 1166, 1455, 1949, 2051
MbiI CCGCTC 1 cut(s) 809
MboI GATC 5 cut(s) 1015, 1164, 1453, 1947, 2049
MfeI CAATTG 1 cut(s) 1314
MhlI GDGCHC 2 cut(s) 864, 1078
MlsI TGGCCA 1 cut(s) 783
MluI ACGCGT 1 cut(s) 825
MluNI TGGCCA 1 cut(s) 783
MlyI GAGTC 2 cut(s) 77, 2023
MmeI TCCRAC 3 cut(s) 903, 1322, 1431
Mox20I TGGCCA 1 cut(s) 783
Mph1103I ATGCAT 3 cut(s) 1485, 1638, 2438
MroI TCCGGA 1 cut(s) 1644
MroXI GAANNNNTTC 2 cut(s) 2111, 2221
MscI TGGCCA 1 cut(s) 783
MslI CAYNNNNRTG 3 cut(s) 1236, 1488, 1923
Msp20I TGGCCA 1 cut(s) 783
MspCI CTTAAG 1 cut(s) 2116
MspI CCGG 2 cut(s) 30, 1645
MspR9I CCNGG 2 cut(s) 1043, 1193
MunI CAATTG 1 cut(s) 1314
MvaI CCWGG 2 cut(s) 1043, 1193
MvnI CGCG 1 cut(s) 827
MwoI GCNNNNNNNGC 5 cut(s) 655, 691, 760, 829, 1228
NdeII GATC 5 cut(s) 1015, 1164, 1453, 1947, 2049
NlaIV GGNNCC 2 cut(s) 518, 1643
NmeAIII GCCGAG 2 cut(s) 22, 1086
NmuCI GTSAC 5 cut(s) 290, 982, 1195, 2035, 2359
NsiI ATGCAT 3 cut(s) 1485, 1638, 2438
NspI RCATGY 3 cut(s) 1483, 1903, 2247
NspV TTCGAA 1 cut(s) 25
PaeR7I CTCGAG 1 cut(s) 863
PciI ACATGT 1 cut(s) 1899
PdmI GAANNNNTTC 2 cut(s) 2111, 2221
PfeI GAWTC 5 cut(s) 209, 421, 2281, 2326, 2332
PflFI GACNNNGTC 1 cut(s) 1193
PflMI CCANNNNNTGG 1 cut(s) 790
PkrI GCNGC 2 cut(s) 40, 1879
PleI GAGTC 2 cut(s) 76, 2023
PpsI GAGTC 2 cut(s) 76, 2023
PpuMI RGGWCCY 1 cut(s) 939
PscI ACATGT 1 cut(s) 1899
PshAI GACNNNNGTC 1 cut(s) 2033
PshBI ATTAAT 1 cut(s) 1728
PsiI TTATAA 2 cut(s) 990, 1203
Psp1406I AACGTT 1 cut(s) 1780
Psp5II RGGWCCY 1 cut(s) 939
Psp6I CCWGG 2 cut(s) 1041, 1191
PspGI CCWGG 2 cut(s) 1041, 1191
PspN4I GGNNCC 2 cut(s) 518, 1643
PspPI GGNCC 3 cut(s) 516, 745, 939
PspPPI RGGWCCY 1 cut(s) 939
PspXI VCTCGAGB 1 cut(s) 863
PsyI GACNNNGTC 1 cut(s) 1193
RsaI GTAC 2 cut(s) 674, 1320
RsaNI GTAC 2 cut(s) 673, 1319
RseI CAYNNNNRTG 3 cut(s) 1236, 1488, 1923
SalI GTCGAC 1 cut(s) 1437
SatI GCNGC 2 cut(s) 39, 1878
Sau3AI GATC 5 cut(s) 1015, 1164, 1453, 1947, 2049
Sau96I GGNCC 3 cut(s) 516, 745, 939
SchI GAGTC 2 cut(s) 77, 2023
ScrFI CCNGG 2 cut(s) 1043, 1193
SduI GDGCHC 2 cut(s) 864, 1078
SexAI ACCWGGT 1 cut(s) 1191
Sfr274I CTCGAG 1 cut(s) 863
SfuI TTCGAA 1 cut(s) 25
SinI GGWCC 2 cut(s) 516, 939
SlaI CTCGAG 1 cut(s) 863
SmiMI CAYNNNNRTG 3 cut(s) 1236, 1488, 1923
SmlI CTYRAG 4 cut(s) 148, 686, 863, 2116
SmoI CTYRAG 4 cut(s) 148, 686, 863, 2116
SsiI CCGC 6 cut(s) 260, 345, 649, 754, 809, 857
SspMI CTAG 2 cut(s) 723, 2378
StyD4I CCNGG 2 cut(s) 1041, 1191
StyI CCWWGG 2 cut(s) 715, 942
TaaI ACNGT 7 cut(s) 296, 995, 1102, 1510, 1681, 1840, 2418
TaiI ACGT 1 cut(s) 1783
TaqI TCGA 7 cut(s) 25, 250, 805, 864, 1438, 2181, 2311
TatI WGTACW 2 cut(s) 672, 1318
TfiI GAWTC 5 cut(s) 209, 421, 2281, 2326, 2332
TscAI CASTG 3 cut(s) 650, 1420, 1957
TseFI GTSAC 5 cut(s) 290, 982, 1195, 2035, 2359
TseI GCWGC 2 cut(s) 38, 1877
Tsp45I GTSAC 5 cut(s) 290, 982, 1195, 2035, 2359
TspRI CASTG 3 cut(s) 650, 1420, 1957
Tth111I GACNNNGTC 1 cut(s) 1193
Van91I CCANNNNNTGG 1 cut(s) 790
Vha464I CTTAAG 1 cut(s) 2116
VpaK11BI GGWCC 2 cut(s) 516, 939
VspI ATTAAT 1 cut(s) 1728
XagI CCTNNNNNAGG 2 cut(s) 946, 1118
XapI RAATTY 3 cut(s) 391, 1256, 1552
XceI RCATGY 3 cut(s) 1483, 1903, 2247
XhoI CTCGAG 1 cut(s) 863
XmiI GTMKAC 1 cut(s) 1438
XmnI GAANNNNTTC 2 cut(s) 2111, 2221
XspI CTAG 2 cut(s) 723, 2378
Zsp2I ATGCAT 3 cut(s) 1485, 1638, 2438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.