Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0005074

Physical Location & Seq

Genomic Coordinates Reverse (-)

38977258 .. 38982196

Gene Structure 4939 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1273000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1710 bp

ATGGCTGGTGATTCCATGCCTTCAAGTGCCCAGCTCGCGAAAGTAACCTTAAGTGACGATTCCTCCGTTCTAAAAGCTGAGTTTTCTGACAGAGTCCTCATCCGTTCCATTATTTCGCGACCTGATGGAGGGTCCAGGTTGGCCGGGCAGACAGTCCGGATTGGTGGGTGGGTGAAGACTGGTCGGAAGGCAGACAAGGATGCATTTGCATTCCTGGAGCTGAACGATGGGTCATGCGCAGGCAACCTTCAGGTGATTGTAAAAGCTGACAAGTATGACCTTGGGCAGCTTGTACAGACTGGTACTTGCCTGTCTGTGGATGGTATGCTTAAGCTTCCCCCTGATGGGAAAAAGCAGAAGGTGGAGCTTGATGTTGAGAAGGTGATCCATGTGGGTCCGGTTGACCCAGCTAAATACCCGTTGCCCAAGACCAGGCTCACCCTCGAGTTTTTGAGGGACCGTCTTCATATGCGTTCCAGAACCAACACTATCTCTGCAGTTACTCGAATCAGAGATGCCTTGGCATTTGCAACCCACTCATTTTTCCATAAGCATGGATTTCTTTATGTGCAAACTCCAATTATTACCACCAGTGACTGTGAGGGTGCTGGTGAGATGTTTCAAGTCACAACCTTGATTAGTGGAGCTGAAAAGTTGGAAAAGGATCTGATTGAGAACCCTCCTCCATCAGAAGCAAACATAGAGGCTGCCAAGCTTAATGTCAAGCAGAGAGGTGATGATGTCGCTCAGCTGAAATCTGCTAAAGCAAGTAAGCAGGATATTGATGCTGCTGTGGTTGAGCTTAAAAAGGCAAAGGATAATGTCTTAAAGCTAGAGGAGCGATCTAAGCTTCAACCTGGTATCCCTCGAAAGGATGGGAAGGTTGATTATACCCAAGATTTCTTCGCCCGTCAAGCCTTTTTGACTGTTTCTGGCCAGTTACAAGTTGAATCTTATGCGTGTGCTCTTAGTAGTGTCTATACATTTGGGCCTACTTTTCGAGCTGAGAACTCCCATACTTCAAGGCATTTGGCAGAGTTTTGGATGGTGGAGCCAGAAATTGCATTTGCAGAACTTGAGGATGATATGAATTGTGCAGAGGCATATGTGAAATTTCTGTGTCAGTGGTTACTTGACGAATGTAGGGAAGACATGGAATTTATGGCTGACAAAATTGATAAAACTTGCATTGATCGTCTAGCAATGGTTGCTAAAACACCCTTTAAACGGATTAGTTATACAGAAGCAGTGGATCTGCTAATTGATGCTGTGAAAAAGGGTAAGAAGTTTGAGAATCATGTAGAGTGGGGGATTGATTTGGCATCTGAGCACGAAAGATACCTGACAGAGGTATTATTTCAAAAGCCTGTTATCGTTTACAACTATCCTAAAGGGATCAAAGCATTCTACATGAGGCTCAACGATGATTTGAAAACAGTGGCTGCTATGGATGTCTTGGTACCAAAGGTGGGAGAGTTGATTGGGGGAAGCCAAAGGGAAGAGCGTTACGATGTTATTATCAGCAGGATTGAGGAGATGGGTCTGCCTGTTGAGCCATATGAGTGGTACCTTGACTTGCGGCGCTTCGGGACTGTCAAACATTGTGGTTTTGGTTTAGGATTCGAACGAATGGTTCTGTTTGCTACCGGGATTGACAACATTAGAGATGTGATTCCTTTCCCTAGATATCCAGGAAGGGCAGATCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0007275 GO:0008150 GO:0009507 GO:0009536 GO:0009790 GO:0009791 GO:0009793 GO:0010035 GO:0010038 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

WHEP-TRS tRNA-synt_2 tRNA_anti-codon

Protein Analysis

570

Amino Acids

63.83

Weight (kDa)

5.94

Isoelectric Point (pI)

32.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA_anti-codon	PF01336	52 - 128	4.1e-08	OB-fold nucleic acid binding domain
tRNA-synt_2	PF00152	154 - 211	1.6e-06	tRNA synthetases class II (D, K and N)
tRNA-synt_2	PF00152	300 - 562	7.1e-50	tRNA synthetases class II (D, K and N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005074)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70980 AT5G56680
fragaria_vesca	FvH4_3g17430
malus_domestica	MD01G1115900.v1.1 MD03G1251800.v1.1 MD05G1158600.v1.1 MD11G1273000.v1.1
prunus_persica	Prupe.4G154500_v2.0.a1 Prupe.4G154500_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1
pyrus_communis	pycom03g19910 pycom05g15060 pycom11g24090
rosa_chinensis	RchiOBHm_Chr5g0028961
rosa_laevigata	RLG00000033131
rosa_multiflora	Rmu_co7984584.1_g000001 Rmu_sc0009042.1_g000005
rosa_roxburghii	Rroxscaffold_1G00050930
rosa_rugosa	Rorug05G0111900 Rorug05G0112000 Rorug05G0112000
rosa_samantha	Rh5AG204000 Rh5BG202300 Rh5CG223100 Rh5DG206100
rosa_wichuraiana	Rw5G018490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	238
Acc65I	GGTACC	2 cut(s)	1459, 1566
AccB1I	GGYRCC	2 cut(s)	1459, 1566
AccII	CGCG	2 cut(s)	38, 118
AccIII	TCCGGA	1 cut(s)	156
AciI	CCGC	1 cut(s)	1579
AclWI	GGATC	4 cut(s)	379, 672, 1260, 1403
AcoI	YGGCCR	2 cut(s)	141, 934
AcsI	RAATTY	2 cut(s)	1112, 1157
AcuI	CTGAAG	1 cut(s)	233
AfaI	GTAC	4 cut(s)	294, 304, 1461, 1568
AfiI	CCNNNNNNNGG	9 cut(s)	128, 316, 344, 345, 432, 871, 1227, 1348, 1469
AflII	CTTAAG	2 cut(s)	49, 329
AgsI	TTSAA	7 cut(s)	24, 623, 854, 950, 1023, 1361, 1432
AjnI	CCWGG	5 cut(s)	134, 213, 431, 856, 1690
Alw21I	GWGCWC	2 cut(s)	967, 1332
AlwI	GGATC	4 cut(s)	379, 672, 1260, 1403
AlwNI	CAGNNNCTG	2 cut(s)	597, 1442
Ama87I	CYCGRG	1 cut(s)	443
Aor13HI	TCCGGA	1 cut(s)	156
AoxI	GGCC	3 cut(s)	141, 934, 989
ApeKI	GCWGC	4 cut(s)	286, 707, 788, 1442
ApoI	RAATTY	2 cut(s)	1112, 1157
Asp718I	GGTACC	2 cut(s)	1459, 1566
AspLEI	GCGC	2 cut(s)	239, 1584
AspS9I	GGNCC	4 cut(s)	132, 395, 457, 989
AsuC2I	CCSGG	2 cut(s)	145, 1648
AsuHPI	GGTGA	7 cut(s)	20, 184, 265, 394, 430, 623, 746
AsuII	TTCGAA	1 cut(s)	1623
AvaI	CYCGRG	1 cut(s)	443
AvaII	GGWCC	3 cut(s)	132, 395, 457
BaeGI	GKGCMC	1 cut(s)	31
BalI	TGGCCA	1 cut(s)	936
BanI	GGYRCC	2 cut(s)	1459, 1566
BbsI	GAAGAC	3 cut(s)	182, 455, 1155
Bbv12I	GWGCWC	2 cut(s)	967, 1332
BbvI	GCAGC	4 cut(s)	298, 694, 775, 1429
BccI	CCATC	8 cut(s)	119, 221, 314, 338, 694, 869, 1039, 1531
BciT130I	CCWGG	5 cut(s)	136, 215, 433, 858, 1692
BciVI	GTATCC	1 cut(s)	872
BcnI	CCSGG	2 cut(s)	145, 1648
BfaI	CTAG	3 cut(s)	833, 1199, 1683
BfmI	CTRYAG	1 cut(s)	495
BfoI	RGCGCY	1 cut(s)	1585
BfrI	CTTAAG	2 cut(s)	49, 329
BfuI	GTATCC	1 cut(s)	872
BglII	AGATCT	1 cut(s)	1702
BisI	GCNGC	5 cut(s)	287, 708, 789, 1443, 1580
BlpI	GCTNAGC	1 cut(s)	747
BlsI	GCNGC	5 cut(s)	288, 709, 790, 1444, 1581
Bme1390I	CCNGG	7 cut(s)	136, 145, 215, 433, 858, 1648, 1692
Bme18I	GGWCC	3 cut(s)	132, 395, 457
BmeT110I	CYCGRG	1 cut(s)	443
BmgT120I	GGNCC	4 cut(s)	132, 395, 457, 989
BmiI	GGNNCC	6 cut(s)	133, 396, 458, 1053, 1461, 1568
BmrFI	CCNGG	7 cut(s)	136, 145, 215, 433, 858, 1648, 1692
BmsI	GCATC	5 cut(s)	190, 505, 775, 1255, 1331
BpiI	GAAGAC	3 cut(s)	182, 455, 1155
BpmI	CTGGAG	1 cut(s)	236
Bpu1102I	GCTNAGC	1 cut(s)	747
Bpu14I	TTCGAA	1 cut(s)	1623
BpuEI	CTTGAG	1 cut(s)	1097
BpuMI	CCSGG	2 cut(s)	145, 1648
BsaJI	CCNNGG	2 cut(s)	280, 519
BsaWI	WCCGGW	2 cut(s)	156, 397
BsaXI	ACNNNNNCTCC	2 cut(s)	47, 77
Bsc4I	CCNNNNNNNGG	9 cut(s)	128, 316, 344, 345, 432, 871, 1227, 1348, 1469
Bse1I	ACTGG	4 cut(s)	184, 304, 591, 937
Bse3DI	GCAATG	1 cut(s)	1209
BseAI	TCCGGA	1 cut(s)	156
BseBI	CCWGG	5 cut(s)	136, 215, 433, 858, 1692
BseDI	CCNNGG	2 cut(s)	280, 519
BseGI	GGATG	7 cut(s)	99, 205, 325, 880, 1050, 1087, 1456
BseLI	CCNNNNNNNGG	9 cut(s)	128, 316, 344, 345, 432, 871, 1227, 1348, 1469
BseMI	GCAATG	1 cut(s)	1209
BseMII	CTCAG	4 cut(s)	69, 761, 996, 1317
BseNI	ACTGG	4 cut(s)	184, 304, 591, 937
BseRI	GAGGAG	3 cut(s)	672, 851, 1547
BseSI	GKGCMC	1 cut(s)	31
BseXI	GCAGC	4 cut(s)	298, 694, 775, 1429
BseYI	CCCAGC	2 cut(s)	30, 406
BsgI	GTGCAG	1 cut(s)	1116
Bsh1236I	CGCG	2 cut(s)	38, 118
BshFI	GGCC	3 cut(s)	143, 936, 991
BshNI	GGYRCC	2 cut(s)	1459, 1566
BsiHKAI	GWGCWC	2 cut(s)	967, 1332
BsiHKCI	CYCGRG	1 cut(s)	443
BsiSI	CCGG	4 cut(s)	144, 157, 398, 1647
BslFI	GGGAC	2 cut(s)	470, 1603
BslI	CCNNNNNNNGG	9 cut(s)	128, 316, 344, 345, 432, 871, 1227, 1348, 1469
BsmFI	GGGAC	2 cut(s)	470, 1603
BsmI	GAATGC	2 cut(s)	209, 1403
BsnI	GGCC	3 cut(s)	143, 936, 991
BsoBI	CYCGRG	1 cut(s)	443
Bsp119I	TTCGAA	1 cut(s)	1623
Bsp1286I	GDGCHC	3 cut(s)	31, 967, 1332
Bsp13I	TCCGGA	1 cut(s)	156
Bsp1407I	TGTACA	1 cut(s)	292
Bsp143I	GATC	7 cut(s)	384, 664, 842, 1192, 1252, 1395, 1702
Bsp1720I	GCTNAGC	1 cut(s)	747
Bsp68I	TCGCGA	2 cut(s)	38, 118
BspACI	CCGC	1 cut(s)	1579
BspANI	GGCC	3 cut(s)	143, 936, 991
BspCNI	CTCAG	4 cut(s)	70, 760, 997, 1318
BspEI	TCCGGA	1 cut(s)	156
BspFNI	CGCG	2 cut(s)	38, 118
BspLI	GGNNCC	6 cut(s)	133, 396, 458, 1053, 1461, 1568
BspMAI	CTGCAG	1 cut(s)	499
BspPI	GGATC	4 cut(s)	379, 672, 1260, 1403
BspQI	GCTCTTC	1 cut(s)	1494
BspT104I	TTCGAA	1 cut(s)	1623
BspT107I	GGYRCC	2 cut(s)	1459, 1566
BspTI	CTTAAG	2 cut(s)	49, 329
BsrDI	GCAATG	1 cut(s)	1209
BsrGI	TGTACA	1 cut(s)	292
BsrI	ACTGG	4 cut(s)	184, 304, 591, 937
BssECI	CCNNGG	2 cut(s)	280, 519
BssMI	GATC	7 cut(s)	384, 664, 842, 1192, 1252, 1395, 1702
BssT1I	CCWWGG	2 cut(s)	280, 519
Bst2UI	CCWGG	5 cut(s)	136, 215, 433, 858, 1692
Bst4CI	ACNGT	6 cut(s)	154, 461, 599, 928, 1438, 1594
Bst6I	CTCTTC	1 cut(s)	1494
BstAFI	CTTAAG	2 cut(s)	49, 329
BstAPI	GCANNNNNTGC	1 cut(s)	1208
BstAUI	TGTACA	1 cut(s)	292
BstBI	TTCGAA	1 cut(s)	1623
BstC8I	GCNNGC	2 cut(s)	36, 241
BstDEI	CTNAG	6 cut(s)	78, 747, 846, 968, 1005, 1326
BstENI	CCTNNNNNAGG	2 cut(s)	126, 1346
BstF5I	GGATG	7 cut(s)	99, 205, 325, 880, 1050, 1087, 1456
BstFNI	CGCG	2 cut(s)	38, 118
BstH2I	RGCGCY	1 cut(s)	1585
BstHHI	GCGC	2 cut(s)	239, 1584
BstKTI	GATC	7 cut(s)	387, 667, 845, 1195, 1255, 1398, 1705
BstMBI	GATC	7 cut(s)	384, 664, 842, 1192, 1252, 1395, 1702
BstMWI	GCNNNNNNNGC	6 cut(s)	35, 838, 847, 914, 1208, 1552
BstNI	CCWGG	5 cut(s)	136, 215, 433, 858, 1692
BstSCI	CCNGG	7 cut(s)	134, 143, 213, 431, 856, 1646, 1690
BstSFI	CTRYAG	1 cut(s)	495
BstSLI	GKGCMC	1 cut(s)	31
BstUI	CGCG	2 cut(s)	38, 118
BstV1I	GCAGC	4 cut(s)	298, 694, 775, 1429
BstV2I	GAAGAC	3 cut(s)	182, 455, 1155
BstX2I	RGATCY	3 cut(s)	664, 1252, 1702
BstXI	CCANNNNNNTGG	2 cut(s)	554, 1563
BstYI	RGATCY	3 cut(s)	664, 1252, 1702
BsuI	GTATCC	1 cut(s)	872
BsuRI	GGCC	3 cut(s)	143, 936, 991
BtsCI	GGATG	7 cut(s)	99, 205, 325, 880, 1050, 1087, 1456
BtsI	GCAGTG	1 cut(s)	1254
BtsIMutI	CAGTG	4 cut(s)	598, 1130, 1254, 1443
BtuMI	TCGCGA	2 cut(s)	38, 118
Cac8I	GCNNGC	2 cut(s)	36, 241
CaiI	CAGNNNCTG	2 cut(s)	597, 1442
CfoI	GCGC	2 cut(s)	239, 1584
Cfr13I	GGNCC	4 cut(s)	132, 395, 457, 989
CsiI	ACCWGGT	1 cut(s)	856
Csp6I	GTAC	4 cut(s)	293, 303, 1460, 1567
CspCI	CAANNNNNGTGG	2 cut(s)	1585, 1620
CviAII	CATG	7 cut(s)	16, 234, 389, 554, 1153, 1298, 1411
CviQI	GTAC	4 cut(s)	293, 303, 1460, 1567
DdeI	CTNAG	6 cut(s)	78, 747, 846, 968, 1005, 1326
DpnI	GATC	7 cut(s)	386, 666, 844, 1194, 1254, 1397, 1704
DpnII	GATC	7 cut(s)	384, 664, 842, 1192, 1252, 1395, 1702
DraI	TTTAAA	1 cut(s)	1225
EaeI	YGGCCR	2 cut(s)	141, 934
Eam1104I	CTCTTC	1 cut(s)	1494
EarI	CTCTTC	1 cut(s)	1494
Eco130I	CCWWGG	2 cut(s)	280, 519
Eco32I	GATATC	1 cut(s)	1688
Eco47I	GGWCC	3 cut(s)	132, 395, 457
Eco57I	CTGAAG	1 cut(s)	233
Eco88I	CYCGRG	1 cut(s)	443
EcoNI	CCTNNNNNAGG	2 cut(s)	126, 1346
EcoRII	CCWGG	5 cut(s)	134, 213, 431, 856, 1690
EcoRV	GATATC	1 cut(s)	1688
EcoT14I	CCWWGG	2 cut(s)	280, 519
EcoT22I	ATGCAT	1 cut(s)	205
ErhI	CCWWGG	2 cut(s)	280, 519
FaeI	CATG	7 cut(s)	19, 237, 392, 557, 1156, 1301, 1414
FaqI	GGGAC	2 cut(s)	470, 1603
FatI	CATG	7 cut(s)	15, 233, 388, 553, 1152, 1297, 1410
FauNDI	CATATG	3 cut(s)	468, 1105, 1558
Fnu4HI	GCNGC	5 cut(s)	287, 708, 789, 1443, 1580
FokI	GGATG	7 cut(s)	86, 212, 332, 887, 1057, 1094, 1463
Fsp4HI	GCNGC	5 cut(s)	287, 708, 789, 1443, 1580
FspBI	CTAG	3 cut(s)	833, 1199, 1683
FspI	TGCGCA	1 cut(s)	238
GlaI	GCGC	2 cut(s)	238, 1583
GluI	GCNGC	5 cut(s)	287, 708, 789, 1443, 1580
GsaI	CCCAGC	2 cut(s)	34, 410
GsuI	CTGGAG	1 cut(s)	236
HaeII	RGCGCY	1 cut(s)	1585
HaeIII	GGCC	3 cut(s)	143, 936, 991
HapII	CCGG	4 cut(s)	144, 157, 398, 1647
HhaI	GCGC	2 cut(s)	239, 1584
Hin1II	CATG	7 cut(s)	19, 237, 392, 557, 1156, 1301, 1414
Hin6I	GCGC	2 cut(s)	237, 1582
HinP1I	GCGC	2 cut(s)	237, 1582
HincII	GTYRAC	1 cut(s)	403
HindII	GTYRAC	1 cut(s)	403
HindIII	AAGCTT	3 cut(s)	332, 713, 848
HinfI	GANTC	8 cut(s)	11, 59, 93, 507, 950, 1294, 1620, 1672
HpaII	CCGG	4 cut(s)	144, 157, 398, 1647
HphI	GGTGA	7 cut(s)	20, 184, 265, 394, 430, 623, 746
Hpy166II	GTNNAC	2 cut(s)	403, 1378
Hpy188I	TCNGA	6 cut(s)	88, 186, 512, 669, 691, 1327
Hpy188III	TCNNGA	5 cut(s)	37, 117, 157, 477, 1588
Hpy8I	GTNNAC	2 cut(s)	403, 1378
HpyAV	CCTTC	7 cut(s)	30, 181, 257, 352, 373, 874, 1689
HpyCH4III	ACNGT	6 cut(s)	154, 461, 599, 928, 1438, 1594
HpyCH4V	TGCA	9 cut(s)	203, 209, 497, 530, 571, 1064, 1070, 1097, 1188
HpyF10VI	GCNNNNNNNGC	6 cut(s)	35, 838, 847, 914, 1208, 1552
HpyF3I	CTNAG	6 cut(s)	78, 747, 846, 968, 1005, 1326
Hsp92II	CATG	7 cut(s)	19, 237, 392, 557, 1156, 1301, 1414
HspAI	GCGC	2 cut(s)	237, 1582
Kpn2I	TCCGGA	1 cut(s)	156
KpnI	GGTACC	2 cut(s)	1463, 1570
Kzo9I	GATC	7 cut(s)	384, 664, 842, 1192, 1252, 1395, 1702
LguI	GCTCTTC	1 cut(s)	1494
LmnI	GCTCC	5 cut(s)	217, 364, 644, 838, 1051
Lsp1109I	GCAGC	4 cut(s)	298, 694, 775, 1429
LweI	GCATC	5 cut(s)	190, 505, 775, 1255, 1331
MabI	ACCWGGT	1 cut(s)	856
MaeI	CTAG	3 cut(s)	833, 1199, 1683
MaeIII	GTNAC	8 cut(s)	43, 53, 499, 593, 625, 939, 1128, 1505
MalI	GATC	7 cut(s)	386, 666, 844, 1194, 1254, 1397, 1704
MboI	GATC	7 cut(s)	384, 664, 842, 1192, 1252, 1395, 1702
MboII	GAAGA	5 cut(s)	187, 455, 895, 1160, 1511
MflI	RGATCY	3 cut(s)	664, 1252, 1702
MhlI	GDGCHC	3 cut(s)	31, 967, 1332
MlsI	TGGCCA	1 cut(s)	936
MluCI	AATT	7 cut(s)	579, 1059, 1090, 1112, 1157, 1173, 1260
MluNI	TGGCCA	1 cut(s)	936
MlyI	GAGTC	1 cut(s)	102
MmeI	TCCRAC	2 cut(s)	164, 636
Mox20I	TGGCCA	1 cut(s)	936
Mph1103I	ATGCAT	1 cut(s)	205
MroI	TCCGGA	1 cut(s)	156
MscI	TGGCCA	1 cut(s)	936
MseI	TTAA	6 cut(s)	50, 330, 717, 804, 827, 1224
MslI	CAYNNNNRTG	2 cut(s)	552, 1561
Msp20I	TGGCCA	1 cut(s)	936
MspA1I	CMGCKG	1 cut(s)	751
MspCI	CTTAAG	2 cut(s)	49, 329
MspI	CCGG	4 cut(s)	144, 157, 398, 1647
MspR9I	CCNGG	7 cut(s)	136, 145, 215, 433, 858, 1648, 1692
Mva1269I	GAATGC	2 cut(s)	209, 1403
MvaI	CCWGG	5 cut(s)	136, 215, 433, 858, 1692
MvnI	CGCG	2 cut(s)	38, 118
MwoI	GCNNNNNNNGC	6 cut(s)	35, 838, 847, 914, 1208, 1552
NciI	CCSGG	2 cut(s)	145, 1648
NdeI	CATATG	3 cut(s)	468, 1105, 1558
NdeII	GATC	7 cut(s)	384, 664, 842, 1192, 1252, 1395, 1702
NlaIII	CATG	7 cut(s)	19, 237, 392, 557, 1156, 1301, 1414
NlaIV	GGNNCC	6 cut(s)	133, 396, 458, 1053, 1461, 1568
NmuCI	GTSAC	3 cut(s)	53, 593, 625
NruI	TCGCGA	2 cut(s)	38, 118
NsbI	TGCGCA	1 cut(s)	238
NsiI	ATGCAT	1 cut(s)	205
NspV	TTCGAA	1 cut(s)	1623
PaeR7I	CTCGAG	1 cut(s)	443
PciSI	GCTCTTC	1 cut(s)	1494
PcsI	WCGNNNNNNNCGW	1 cut(s)	63
PctI	GAATGC	2 cut(s)	209, 1403
PfeI	GAWTC	7 cut(s)	11, 59, 507, 950, 1294, 1620, 1672
PflFI	GACNNNGTC	1 cut(s)	92
PfoI	TCCNGGA	2 cut(s)	213, 1690
PkrI	GCNGC	5 cut(s)	288, 709, 790, 1444, 1581
PleI	GAGTC	1 cut(s)	101
PpsI	GAGTC	1 cut(s)	101
Psp6I	CCWGG	5 cut(s)	134, 213, 431, 856, 1690
PspFI	CCCAGC	2 cut(s)	30, 406
PspGI	CCWGG	5 cut(s)	134, 213, 431, 856, 1690
PspN4I	GGNNCC	6 cut(s)	133, 396, 458, 1053, 1461, 1568
PspPI	GGNCC	4 cut(s)	132, 395, 457, 989
PspXI	VCTCGAGB	1 cut(s)	443
PstI	CTGCAG	1 cut(s)	499
PstNI	CAGNNNCTG	2 cut(s)	597, 1442
PsuI	RGATCY	3 cut(s)	664, 1252, 1702
PsyI	GACNNNGTC	1 cut(s)	92
PvuII	CAGCTG	1 cut(s)	751
RruI	TCGCGA	2 cut(s)	38, 118
RsaI	GTAC	4 cut(s)	294, 304, 1461, 1568
RsaNI	GTAC	4 cut(s)	293, 303, 1460, 1567
RseI	CAYNNNNRTG	2 cut(s)	552, 1561
SapI	GCTCTTC	1 cut(s)	1494
SaqAI	TTAA	6 cut(s)	50, 330, 717, 804, 827, 1224
SatI	GCNGC	5 cut(s)	287, 708, 789, 1443, 1580
Sau3AI	GATC	7 cut(s)	384, 664, 842, 1192, 1252, 1395, 1702
Sau96I	GGNCC	4 cut(s)	132, 395, 457, 989
SchI	GAGTC	1 cut(s)	102
ScrFI	CCNGG	7 cut(s)	136, 145, 215, 433, 858, 1648, 1692
SduI	GDGCHC	3 cut(s)	31, 967, 1332
SexAI	ACCWGGT	1 cut(s)	856
SfaNI	GCATC	5 cut(s)	190, 505, 775, 1255, 1331
SfcI	CTRYAG	1 cut(s)	495
Sfr274I	CTCGAG	1 cut(s)	443
SfuI	TTCGAA	1 cut(s)	1623
SinI	GGWCC	3 cut(s)	132, 395, 457
SlaI	CTCGAG	1 cut(s)	443
SmiMI	CAYNNNNRTG	2 cut(s)	552, 1561
SmlI	CTYRAG	4 cut(s)	49, 329, 443, 1076
SmoI	CTYRAG	4 cut(s)	49, 329, 443, 1076
Sse9I	AATT	7 cut(s)	579, 1059, 1090, 1112, 1157, 1173, 1260
SsiI	CCGC	1 cut(s)	1579
SspMI	CTAG	3 cut(s)	833, 1199, 1683
StyD4I	CCNGG	7 cut(s)	134, 143, 213, 431, 856, 1646, 1690
StyI	CCWWGG	2 cut(s)	280, 519
TaaI	ACNGT	6 cut(s)	154, 461, 599, 928, 1438, 1594
TaqI	TCGA	5 cut(s)	444, 505, 868, 1000, 1623
TasI	AATT	7 cut(s)	579, 1059, 1090, 1112, 1157, 1173, 1260
TatI	WGTACW	1 cut(s)	292
TauI	GCSGC	1 cut(s)	1582
TfiI	GAWTC	7 cut(s)	11, 59, 507, 950, 1294, 1620, 1672
Tru1I	TTAA	6 cut(s)	50, 330, 717, 804, 827, 1224
Tru9I	TTAA	6 cut(s)	50, 330, 717, 804, 827, 1224
TscAI	CASTG	4 cut(s)	598, 1130, 1254, 1443
TseFI	GTSAC	3 cut(s)	53, 593, 625
TseI	GCWGC	4 cut(s)	286, 707, 788, 1442
Tsp45I	GTSAC	3 cut(s)	53, 593, 625
TspDTI	ATGAA	2 cut(s)	455, 1103
TspGWI	ACGGA	3 cut(s)	55, 92, 1243
TspRI	CASTG	4 cut(s)	598, 1130, 1254, 1443
Tth111I	GACNNNGTC	1 cut(s)	92
Vha464I	CTTAAG	2 cut(s)	49, 329
VpaK11BI	GGWCC	3 cut(s)	132, 395, 457
XagI	CCTNNNNNAGG	2 cut(s)	126, 1346
XapI	RAATTY	2 cut(s)	1112, 1157
XhoI	CTCGAG	1 cut(s)	443
XspI	CTAG	3 cut(s)	833, 1199, 1683
Zsp2I	ATGCAT	1 cut(s)	205

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.