Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0005074

Physical Location & Seq

Genomic Coordinates Forward (+)

28764031 .. 28764888

Gene Structure 858 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1158600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 582 bp

ATGAACTGTGCAGAGAAGTATGTGAAATTCTTGTGTAAGTCCGTACTTAAAGAATGCAAAGAAGATATGCAATTTATGGCTGAAAAATATGTTAAAAGTTGTGTGGGTCGTCTAGAAATTGTCAGATCAACGCCGTTTAAACGGGTTACATATACAGAAGCTGTGGATTTGCTAGTTGAGGCTGTCAAAGATGGTAAGAAGTTTGAGAACCATATACTTGACGAGGAGAACTTTAAGCAGCCGGTTATTGTTTACAACTATCCGAAAAAGACCAAAGCCTTCTATAGGCTCAATGAAGACGGTGAGACAGTGTTTGCTATGGATGTCGTCGTCCCAAAAGTTGGATGGTTGATCGGTGGAAGCCCAAGGGAAGAGTGTTACGGTGTTCTAAAGGAAAGGATTCTGGAGATGGAACGGCACGTTGAGGATTATCAATCGTACCTAGACTTGCGGCGCTTTGGGACTATTAAGCATAGCGGCTTTGATTTAGGGTTTGAACGGATGGTTATGTTTGCCACTGGGCTTGACAACATTAGAGATTGCATTTCCTACCCTAGAGATCGAGGAAGAGAAGATCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0007275 GO:0008150 GO:0009507 GO:0009536 GO:0009790 GO:0009791 GO:0009793 GO:0010035 GO:0010038 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

WHEP-TRS tRNA-synt_2 tRNA_anti-codon

Protein Analysis

194

Amino Acids

22.6

Weight (kDa)

6.35

Isoelectric Point (pI)

42.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2	PF00152	1 - 187	1.4e-28	tRNA synthetases class II (D, K and N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0005074)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70980 AT5G56680
fragaria_vesca	FvH4_3g17430
malus_domestica	MD01G1115900.v1.1 MD03G1251800.v1.1 MD05G1158600.v1.1 MD11G1273000.v1.1
prunus_persica	Prupe.4G154500_v2.0.a1 Prupe.4G154500_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1
pyrus_communis	pycom03g19910 pycom05g15060 pycom11g24090
rosa_chinensis	RchiOBHm_Chr5g0028961
rosa_laevigata	RLG00000033131
rosa_multiflora	Rmu_co7984584.1_g000001 Rmu_sc0009042.1_g000005
rosa_roxburghii	Rroxscaffold_1G00050930
rosa_rugosa	Rorug05G0111900 Rorug05G0112000 Rorug05G0112000
rosa_samantha	Rh5AG204000 Rh5BG202300 Rh5CG223100 Rh5DG206100
rosa_wichuraiana	Rw5G018490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	341
AciI	CCGC	2 cut(s)	451, 477
AclWI	GGATC	1 cut(s)	569
AcsI	RAATTY	1 cut(s)	26
AfaI	GTAC	2 cut(s)	45, 440
AfiI	CCNNNNNNNGG	2 cut(s)	285, 341
AgsI	TTSAA	1 cut(s)	497
AluBI	AGCT	1 cut(s)	161
AluI	AGCT	1 cut(s)	161
Alw26I	GTCTC	1 cut(s)	299
AlwI	GGATC	1 cut(s)	569
AlwNI	CAGNNNCTG	1 cut(s)	161
ApeKI	GCWGC	1 cut(s)	238
ApoI	RAATTY	1 cut(s)	26
Asp700I	GAANNNNTTC	1 cut(s)	399
AspLEI	GCGC	1 cut(s)	456
AsuHPI	GGTGA	1 cut(s)	314
BbsI	GAAGAC	1 cut(s)	303
BbvI	GCAGC	1 cut(s)	250
BccI	CCATC	4 cut(s)	185, 339, 403, 496
BceAI	ACGGC	2 cut(s)	118, 431
BcoDI	GTCTC	1 cut(s)	299
BfaI	CTAG	4 cut(s)	113, 173, 443, 555
BfmI	CTRYAG	1 cut(s)	283
BfoI	RGCGCY	1 cut(s)	457
BisI	GCNGC	3 cut(s)	239, 452, 478
BlsI	GCNGC	3 cut(s)	240, 453, 479
BmrI	ACTGGG	1 cut(s)	528
BmuI	ACTGGG	1 cut(s)	528
BpiI	GAAGAC	1 cut(s)	303
BpmI	CTGGAG	1 cut(s)	425
BsaJI	CCNNGG	1 cut(s)	365
Bsc4I	CCNNNNNNNGG	2 cut(s)	285, 341
Bse118I	RCCGGY	1 cut(s)	241
Bse1I	ACTGG	1 cut(s)	523
BseDI	CCNNGG	1 cut(s)	365
BseGI	GGATG	3 cut(s)	328, 350, 507
BseLI	CCNNNNNNNGG	2 cut(s)	285, 341
BseNI	ACTGG	1 cut(s)	523
BseRI	GAGGAG	1 cut(s)	239
BseXI	GCAGC	1 cut(s)	250
BsgI	GTGCAG	1 cut(s)	30
BsiSI	CCGG	1 cut(s)	242
BslFI	GGGAC	2 cut(s)	317, 475
BslI	CCNNNNNNNGG	2 cut(s)	285, 341
BsmAI	GTCTC	1 cut(s)	299
BsmFI	GGGAC	2 cut(s)	317, 475
BsmI	GAATGC	1 cut(s)	59
Bsp143I	GATC	4 cut(s)	125, 351, 559, 574
BspACI	CCGC	2 cut(s)	451, 477
BspPI	GGATC	1 cut(s)	569
BsrFI	RCCGGY	1 cut(s)	241
BsrI	ACTGG	1 cut(s)	523
BssAI	RCCGGY	1 cut(s)	241
BssECI	CCNNGG	1 cut(s)	365
BssMI	GATC	4 cut(s)	125, 351, 559, 574
BssT1I	CCWWGG	1 cut(s)	365
Bst4CI	ACNGT	4 cut(s)	8, 302, 310, 383
Bst6I	CTCTTC	2 cut(s)	366, 562
BstENI	CCTNNNNNAGG	1 cut(s)	283
BstF5I	GGATG	3 cut(s)	328, 350, 507
BstH2I	RGCGCY	1 cut(s)	457
BstHHI	GCGC	1 cut(s)	456
BstKTI	GATC	4 cut(s)	128, 354, 562, 577
BstMAI	GTCTC	1 cut(s)	299
BstMBI	GATC	4 cut(s)	125, 351, 559, 574
BstSFI	CTRYAG	1 cut(s)	283
BstV1I	GCAGC	1 cut(s)	250
BstV2I	GAAGAC	1 cut(s)	303
BstX2I	RGATCY	1 cut(s)	574
BstYI	RGATCY	1 cut(s)	574
BtsCI	GGATG	3 cut(s)	328, 350, 507
BtsIMutI	CAGTG	2 cut(s)	315, 516
CaiI	CAGNNNCTG	1 cut(s)	161
CfoI	GCGC	1 cut(s)	456
Cfr10I	RCCGGY	1 cut(s)	241
Csp6I	GTAC	2 cut(s)	44, 439
CspCI	CAANNNNNGTGG	2 cut(s)	505, 540
CviJI	RGCY	9 cut(s)	80, 161, 182, 241, 278, 289, 363, 480, 523
CviKI_1	RGCY	9 cut(s)	80, 161, 182, 241, 278, 289, 363, 480, 523
CviQI	GTAC	2 cut(s)	44, 439
DpnI	GATC	4 cut(s)	127, 353, 561, 576
DpnII	GATC	4 cut(s)	125, 351, 559, 574
DraI	TTTAAA	1 cut(s)	139
Eam1104I	CTCTTC	2 cut(s)	366, 562
EarI	CTCTTC	2 cut(s)	366, 562
Eco130I	CCWWGG	1 cut(s)	365
EcoNI	CCTNNNNNAGG	1 cut(s)	283
EcoT14I	CCWWGG	1 cut(s)	365
ErhI	CCWWGG	1 cut(s)	365
FaqI	GGGAC	2 cut(s)	317, 475
Fnu4HI	GCNGC	3 cut(s)	239, 452, 478
FokI	GGATG	3 cut(s)	335, 357, 514
Fsp4HI	GCNGC	3 cut(s)	239, 452, 478
FspBI	CTAG	4 cut(s)	113, 173, 443, 555
GlaI	GCGC	1 cut(s)	455
GluI	GCNGC	3 cut(s)	239, 452, 478
GsuI	CTGGAG	1 cut(s)	425
HaeII	RGCGCY	1 cut(s)	457
HapII	CCGG	1 cut(s)	242
HhaI	GCGC	1 cut(s)	456
Hin6I	GCGC	1 cut(s)	454
HinP1I	GCGC	1 cut(s)	454
HinfI	GANTC	1 cut(s)	400
HpaII	CCGG	1 cut(s)	242
HphI	GGTGA	1 cut(s)	314
Hpy166II	GTNNAC	1 cut(s)	253
Hpy188I	TCNGA	2 cut(s)	125, 264
Hpy188III	TCNNGA	2 cut(s)	113, 404
Hpy8I	GTNNAC	1 cut(s)	253
Hpy99I	CGWCG	1 cut(s)	332
HpyAV	CCTTC	1 cut(s)	289
HpyCH4III	ACNGT	4 cut(s)	8, 302, 310, 383
HpyCH4IV	ACGT	1 cut(s)	420
HpyCH4V	TGCA	4 cut(s)	11, 57, 70, 543
HpySE526I	ACGT	1 cut(s)	420
HspAI	GCGC	1 cut(s)	454
Kzo9I	GATC	4 cut(s)	125, 351, 559, 574
LpnPI	CCDG	3 cut(s)	255, 389, 504
Lsp1109I	GCAGC	1 cut(s)	250
MaeI	CTAG	4 cut(s)	113, 173, 443, 555
MaeII	ACGT	1 cut(s)	420
MaeIII	GTNAC	2 cut(s)	145, 377
MalI	GATC	4 cut(s)	127, 353, 561, 576
MboI	GATC	4 cut(s)	125, 351, 559, 574
MboII	GAAGA	4 cut(s)	74, 308, 383, 579
MflI	RGATCY	1 cut(s)	574
MluCI	AATT	3 cut(s)	26, 71, 117
MmeI	TCCRAC	1 cut(s)	322
MnlI	CCTC	4 cut(s)	172, 217, 418, 557
MroXI	GAANNNNTTC	1 cut(s)	399
MseI	TTAA	6 cut(s)	48, 93, 138, 234, 468, 580
MspI	CCGG	1 cut(s)	242
MssI	GTTTAAAC	1 cut(s)	139
Mva1269I	GAATGC	1 cut(s)	59
NdeII	GATC	4 cut(s)	125, 351, 559, 574
PctI	GAATGC	1 cut(s)	59
PdmI	GAANNNNTTC	1 cut(s)	399
PfeI	GAWTC	1 cut(s)	400
PflMI	CCANNNNNTGG	1 cut(s)	341
PkrI	GCNGC	3 cut(s)	240, 453, 479
PmeI	GTTTAAAC	1 cut(s)	139
PstNI	CAGNNNCTG	1 cut(s)	161
PsuI	RGATCY	1 cut(s)	574
RsaI	GTAC	2 cut(s)	45, 440
RsaNI	GTAC	2 cut(s)	44, 439
SaqAI	TTAA	6 cut(s)	48, 93, 138, 234, 468, 580
SatI	GCNGC	3 cut(s)	239, 452, 478
Sau3AI	GATC	4 cut(s)	125, 351, 559, 574
SetI	ASST	3 cut(s)	163, 423, 444
SfcI	CTRYAG	1 cut(s)	283
Sse9I	AATT	3 cut(s)	26, 71, 117
SsiI	CCGC	2 cut(s)	451, 477
SspMI	CTAG	4 cut(s)	113, 173, 443, 555
StyI	CCWWGG	1 cut(s)	365
TaaI	ACNGT	4 cut(s)	8, 302, 310, 383
TaiI	ACGT	1 cut(s)	423
TaqI	TCGA	1 cut(s)	562
TasI	AATT	3 cut(s)	26, 71, 117
TauI	GCSGC	2 cut(s)	454, 480
TfiI	GAWTC	1 cut(s)	400
Tru1I	TTAA	6 cut(s)	48, 93, 138, 234, 468, 580
Tru9I	TTAA	6 cut(s)	48, 93, 138, 234, 468, 580
TscAI	CASTG	2 cut(s)	315, 523
TseI	GCWGC	1 cut(s)	238
TspDTI	ATGAA	2 cut(s)	17, 309
TspGWI	ACGGA	2 cut(s)	31, 514
TspRI	CASTG	2 cut(s)	315, 523
Van91I	CCANNNNNTGG	1 cut(s)	341
XagI	CCTNNNNNAGG	1 cut(s)	283
XapI	RAATTY	1 cut(s)	26
XbaI	TCTAGA	1 cut(s)	112
XcmI	CCANNNNNNNNNTGG	1 cut(s)	342
XmnI	GAANNNNTTC	1 cut(s)	399
XspI	CTAG	4 cut(s)	113, 173, 443, 555

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.