Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0005074

Physical Location & Seq

Genomic Coordinates Forward (+)

22785672 .. 22789769

Gene Structure 4098 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG206100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1554 bp

ATGGCTACCGATTCGGCACAGTTAGGTTCCCAACTGGCTGAAGCAACTTTAAACGAATCTGTCTCCGCTCTGAAAGCAGAATTTTCTGACAGAGTTCCCATCAGATCCATAATATCACGAACTGATGGGGGGTCTGGGTTTGCCGGGCAGCATGTCCGGATTGGTGGTTGGGTGAAGACGGGCAGGAAGGCAGACAAGGATGCATTTGCTTTTCTGGAACTTAATGATGGATCATGCCCTGGCAACTTACAGGTGATTGTGGAAGCAGACAAGGGTGACCTTGGGCAGCTTGTGCCAACCGGTACATGTGTGGTTGTGGATGGCGTGCTTAAGCTGCCCCCAGCTGGGGCCAAGCAGAAGGTGGAGCTTAGAGTTGAGAAGGTGGTCCATTTAGGTCCAGTTGACCCTGCTAAATATCCGTTGCCCAAGACCAAGCTCACCCTTGAGTTTTTGAGGGACGTTGTTCATCTCCGTTCCAGAACTAACACCATCTCTGCAGTTGCTCGCATCAGAAATGCCCTGGCATATGCAACTCATACATTTTTCCAAAAGCATGGCTTCCTTTATGTGCACACTCCAATTATCACTACCAGTGATTGTGAGGGTGCTGGTGAAATGTTCCAAGTCACAACATTGATTAGCGAAGGTGAAAGGTTGGAGAGGGAGCTGATTAAGAACCCTGCCCCATCTGAAGCAGACCTAGAAGCTGCCAAGCTAATCATCAAGGAAAAAGGAGATGCTGTTTCACAGCTGAAATCTGCTAAAGCAAGTAAGGAGGAGATTGGTGCTGCAGTCGCTGAACTTAAAAGGGCGAAGGAGAATGCATTGAAGCTGGAGGAGAGATCAAAGCTTCAGCCAGGAATCCCCAAAAAGGATGGGAAGATTGACTATACACAAGATTTCTTTGCCCGTCAAGCTTTTTTGACCGTTTCTGGCCAACTCCAAGTGGAGTCTTATGCATGTGCTCTTAGTAGTGTGTATACATTTGGGCCTACTTTTCGTGCTGAGAATTCACACACTTCACGGCATTTGGCAGAATTCTGGATGGTGGAGCCTGAATTAGCGTTTGCAGAGCTCAAGGATGACATGAACTGTGCAGAGGCGTATGTGAAATTCTTGTGTCAGTGGTTACTTGACAACTGCTCTGACGATATGGAGTTTATTTCTCGGCAATTTGATAAAACTTGCATAGACCGTCTAAAGATGGTTGCATCCACACCGTTTGAACGGATTACATATACAGAAGCTGTGGAGCTGCTAATTGATGCTGTGAAAAATGGCAAGAAGTTTGAGAATCATGTAGAGTGGGGGATTGACTTGGCATCTGAACATGAAAGATTCTTAACAGAGGTGAAATTTCAGAAGCCTGTTATTGTGTACAATTACCCAAAAGGGATCAAAGCTTTCTACATGAGACTCAATGATGATAACAAAACAGTGGCTGCTATGGATGTCCTTGTACCAAAGGTGGGAGAGTTGATTGGGGGTAGCCAAAGGGAAGAACGCTATGATGTGATTCATAGCAGGTATGAGAACTGTGCTGCTTTAAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0007275 GO:0008150 GO:0009507 GO:0009536 GO:0009790 GO:0009791 GO:0009793 GO:0010035 GO:0010038 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

WHEP-TRS tRNA-synt_2 tRNA_anti-codon

Protein Analysis

517

Amino Acids

57.4

Weight (kDa)

5.98

Isoelectric Point (pI)

28.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA_anti-codon	PF01336	52 - 129	1.8e-08	OB-fold nucleic acid binding domain
tRNA-synt_2	PF00152	155 - 212	3.4e-07	tRNA synthetases class II (D, K and N)
WHEP-TRS	PF00458	241 - 274	1.1e-06	WHEP-TRS domain
tRNA-synt_2	PF00152	300 - 511	3.9e-31	tRNA synthetases class II (D, K and N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005074)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70980 AT5G56680
fragaria_vesca	FvH4_3g17430
malus_domestica	MD01G1115900.v1.1 MD03G1251800.v1.1 MD05G1158600.v1.1 MD11G1273000.v1.1
prunus_persica	Prupe.4G154500_v2.0.a1 Prupe.4G154500_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1
pyrus_communis	pycom03g19910 pycom05g15060 pycom11g24090
rosa_chinensis	RchiOBHm_Chr5g0028961
rosa_laevigata	RLG00000033131
rosa_multiflora	Rmu_co7984584.1_g000001 Rmu_sc0009042.1_g000005
rosa_roxburghii	Rroxscaffold_1G00050930
rosa_rugosa	Rorug05G0111900 Rorug05G0112000 Rorug05G0112000
rosa_samantha	Rh5AG204000 Rh5BG202300 Rh5CG223100 Rh5DG206100
rosa_wichuraiana	Rw5G018490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1515
AccBSI	CCGCTC	1 cut(s)	68
AccI	GTMKAC	1 cut(s)	980
AccIII	TCCGGA	1 cut(s)	156
AciI	CCGC	1 cut(s)	66
AclWI	GGATC	3 cut(s)	99, 238, 1403
AcoI	YGGCCR	1 cut(s)	934
AcsI	RAATTY	5 cut(s)	80, 1009, 1037, 1112, 1355
AcuI	CTGAAG	3 cut(s)	60, 711, 836
AfaI	GTAC	3 cut(s)	304, 1379, 1461
AfiI	CCNNNNNNNGG	5 cut(s)	344, 345, 346, 871, 1469
AflII	CTTAAG	1 cut(s)	329
AflIII	ACRYGT	1 cut(s)	305
AgeI	ACCGGT	1 cut(s)	299
AgsI	TTSAA	2 cut(s)	829, 1226
AjnI	CCWGG	3 cut(s)	238, 519, 856
Alw21I	GWGCWC	3 cut(s)	573, 967, 1077
Alw26I	GTCTC	2 cut(s)	67, 1408
Alw44I	GTGCAC	1 cut(s)	569
AlwI	GGATC	3 cut(s)	99, 238, 1403
AlwNI	CAGNNNCTG	3 cut(s)	797, 1247, 1442
Aor13HI	TCCGGA	1 cut(s)	156
AoxI	GGCC	3 cut(s)	348, 934, 989
ApaLI	GTGCAC	1 cut(s)	569
ApeKI	GCWGC	8 cut(s)	148, 286, 334, 707, 788, 1255, 1442, 1541
ApoI	RAATTY	5 cut(s)	80, 1009, 1037, 1112, 1355
AsiGI	ACCGGT	1 cut(s)	299
AspS9I	GGNCC	4 cut(s)	348, 385, 395, 989
AsuC2I	CCSGG	1 cut(s)	145
AsuHPI	GGTGA	7 cut(s)	184, 265, 287, 430, 623, 659, 1363
AvaII	GGWCC	2 cut(s)	385, 395
BaeGI	GKGCMC	1 cut(s)	573
BaeI	ACNNNNGTAYC	2 cut(s)	294, 327
BalI	TGGCCA	1 cut(s)	936
BanII	GRGCYC	1 cut(s)	1077
BbsI	GAAGAC	1 cut(s)	182
Bbv12I	GWGCWC	3 cut(s)	573, 967, 1077
BbvI	GCAGC	8 cut(s)	160, 298, 321, 694, 775, 1242, 1429, 1528
BccI	CCATC	9 cut(s)	107, 119, 221, 314, 497, 694, 869, 1039, 1198
BceAI	ACGGC	1 cut(s)	1040
BciT130I	CCWGG	3 cut(s)	240, 521, 858
BcnI	CCSGG	1 cut(s)	145
BcoDI	GTCTC	2 cut(s)	67, 1408
BfaI	CTAG	1 cut(s)	701
BfmI	CTRYAG	2 cut(s)	495, 789
BfrI	CTTAAG	1 cut(s)	329
BfuAI	ACCTGC	1 cut(s)	1515
BisI	GCNGC	8 cut(s)	149, 287, 335, 708, 789, 1256, 1443, 1542
BlsI	GCNGC	8 cut(s)	150, 288, 336, 709, 790, 1257, 1444, 1543
Bme1390I	CCNGG	4 cut(s)	145, 240, 521, 858
Bme18I	GGWCC	2 cut(s)	385, 395
BmgT120I	GGNCC	4 cut(s)	348, 385, 395, 989
BmiI	GGNNCC	3 cut(s)	28, 349, 1053
BmrFI	CCNGG	4 cut(s)	145, 240, 521, 858
BmsI	GCATC	6 cut(s)	190, 516, 727, 1220, 1255, 1331
BpiI	GAAGAC	1 cut(s)	182
BpmI	CTGGAG	1 cut(s)	854
BpuEI	CTTGAG	2 cut(s)	464, 1061
BpuMI	CCSGG	1 cut(s)	145
BsaJI	CCNNGG	3 cut(s)	238, 280, 519
BsaWI	WCCGGW	2 cut(s)	156, 299
BsaXI	ACNNNNNCTCC	2 cut(s)	726, 756
Bsc4I	CCNNNNNNNGG	5 cut(s)	344, 345, 346, 871, 1469
Bse118I	RCCGGY	1 cut(s)	299
Bse1I	ACTGG	3 cut(s)	39, 398, 591
BseAI	TCCGGA	1 cut(s)	156
BseBI	CCWGG	3 cut(s)	240, 521, 858
BseDI	CCNNGG	3 cut(s)	238, 280, 519
BseGI	GGATG	7 cut(s)	205, 325, 880, 1050, 1087, 1211, 1456
BseLI	CCNNNNNNNGG	5 cut(s)	344, 345, 346, 871, 1469
BseMII	CTCAG	1 cut(s)	996
BseNI	ACTGG	3 cut(s)	39, 398, 591
BseRI	GAGGAG	2 cut(s)	791, 851
BseSI	GKGCMC	1 cut(s)	573
BseXI	GCAGC	8 cut(s)	160, 298, 321, 694, 775, 1242, 1429, 1528
BseYI	CCCAGC	2 cut(s)	340, 344
BsgI	GTGCAG	1 cut(s)	1116
BshFI	GGCC	3 cut(s)	350, 936, 991
BshTI	ACCGGT	1 cut(s)	299
BsiHKAI	GWGCWC	3 cut(s)	573, 967, 1077
BsiSI	CCGG	3 cut(s)	144, 157, 300
BslFI	GGGAC	1 cut(s)	470
BslI	CCNNNNNNNGG	5 cut(s)	344, 345, 346, 871, 1469
BsmAI	GTCTC	2 cut(s)	67, 1408
BsmFI	GGGAC	1 cut(s)	470
BsmI	GAATGC	1 cut(s)	826
BsnI	GGCC	3 cut(s)	350, 936, 991
Bsp1286I	GDGCHC	3 cut(s)	573, 967, 1077
Bsp13I	TCCGGA	1 cut(s)	156
Bsp1407I	TGTACA	1 cut(s)	1377
Bsp143I	GATC	4 cut(s)	104, 230, 842, 1395
BspACI	CCGC	1 cut(s)	66
BspANI	GGCC	3 cut(s)	350, 936, 991
BspCNI	CTCAG	1 cut(s)	997
BspEI	TCCGGA	1 cut(s)	156
BspLI	GGNNCC	3 cut(s)	28, 349, 1053
BspMAI	CTGCAG	2 cut(s)	499, 793
BspMI	ACCTGC	1 cut(s)	1515
BspPI	GGATC	3 cut(s)	99, 238, 1403
BspTI	CTTAAG	1 cut(s)	329
BsrBI	CCGCTC	1 cut(s)	68
BsrFI	RCCGGY	1 cut(s)	299
BsrGI	TGTACA	1 cut(s)	1377
BsrI	ACTGG	3 cut(s)	39, 398, 591
BssAI	RCCGGY	1 cut(s)	299
BssECI	CCNNGG	3 cut(s)	238, 280, 519
BssMI	GATC	4 cut(s)	104, 230, 842, 1395
BssNAI	GTATAC	1 cut(s)	981
BssT1I	CCWWGG	1 cut(s)	280
Bst1107I	GTATAC	1 cut(s)	981
Bst2UI	CCWGG	3 cut(s)	240, 521, 858
Bst4CI	ACNGT	7 cut(s)	21, 928, 1094, 1196, 1221, 1438, 1538
BstAFI	CTTAAG	1 cut(s)	329
BstAPI	GCANNNNNTGC	1 cut(s)	292
BstAUI	TGTACA	1 cut(s)	1377
BstC8I	GCNNGC	2 cut(s)	326, 505
BstDEI	CTNAG	3 cut(s)	368, 968, 1005
BstEII	GGTNACC	1 cut(s)	275
BstF5I	GGATG	7 cut(s)	205, 325, 880, 1050, 1087, 1211, 1456
BstKTI	GATC	4 cut(s)	107, 233, 845, 1398
BstMAI	GTCTC	2 cut(s)	67, 1408
BstMBI	GATC	4 cut(s)	104, 230, 842, 1395
BstMWI	GCNNNNNNNGC	5 cut(s)	74, 292, 334, 794, 914
BstNI	CCWGG	3 cut(s)	240, 521, 858
BstNSI	RCATGY	3 cut(s)	155, 309, 963
BstPI	GGTNACC	1 cut(s)	275
BstSCI	CCNGG	4 cut(s)	143, 238, 519, 856
BstSFI	CTRYAG	2 cut(s)	495, 789
BstSLI	GKGCMC	1 cut(s)	573
BstV1I	GCAGC	8 cut(s)	160, 298, 321, 694, 775, 1242, 1429, 1528
BstV2I	GAAGAC	1 cut(s)	182
BstX2I	RGATCY	1 cut(s)	104
BstXI	CCANNNNNNTGG	1 cut(s)	554
BstYI	RGATCY	1 cut(s)	104
BstZ17I	GTATAC	1 cut(s)	981
BsuRI	GGCC	3 cut(s)	350, 936, 991
BtsCI	GGATG	7 cut(s)	205, 325, 880, 1050, 1087, 1211, 1456
BtsIMutI	CAGTG	3 cut(s)	598, 1130, 1443
BveI	ACCTGC	1 cut(s)	1515
Cac8I	GCNNGC	2 cut(s)	326, 505
CaiI	CAGNNNCTG	3 cut(s)	797, 1247, 1442
Cfr10I	RCCGGY	1 cut(s)	299
Cfr13I	GGNCC	4 cut(s)	348, 385, 395, 989
Csp6I	GTAC	3 cut(s)	303, 1378, 1460
CspAI	ACCGGT	1 cut(s)	299
CspCI	CAANNNNNGTGG	2 cut(s)	1204, 1239
CviAII	CATG	9 cut(s)	152, 234, 306, 554, 960, 1087, 1298, 1331, 1411
CviQI	GTAC	3 cut(s)	303, 1378, 1460
DdeI	CTNAG	3 cut(s)	368, 968, 1005
DpnI	GATC	4 cut(s)	106, 232, 844, 1397
DpnII	GATC	4 cut(s)	104, 230, 842, 1395
DraI	TTTAAA	2 cut(s)	51, 1548
EaeI	YGGCCR	1 cut(s)	934
Ecl136II	GAGCTC	1 cut(s)	1075
Eco130I	CCWWGG	1 cut(s)	280
Eco24I	GRGCYC	1 cut(s)	1077
Eco47I	GGWCC	2 cut(s)	385, 395
Eco53kI	GAGCTC	1 cut(s)	1075
Eco57I	CTGAAG	3 cut(s)	60, 711, 836
Eco91I	GGTNACC	1 cut(s)	275
EcoICRI	GAGCTC	1 cut(s)	1075
EcoO65I	GGTNACC	1 cut(s)	275
EcoRI	GAATTC	2 cut(s)	1009, 1037
EcoRII	CCWGG	3 cut(s)	238, 519, 856
EcoT14I	CCWWGG	1 cut(s)	280
EcoT22I	ATGCAT	3 cut(s)	205, 826, 961
EcoT38I	GRGCYC	1 cut(s)	1077
ErhI	CCWWGG	1 cut(s)	280
FaeI	CATG	9 cut(s)	155, 237, 309, 557, 963, 1090, 1301, 1334, 1414
FalI	AAGNNNNNCTT	2 cut(s)	542, 574
FaqI	GGGAC	1 cut(s)	470
FatI	CATG	9 cut(s)	151, 233, 305, 553, 959, 1086, 1297, 1330, 1410
FauNDI	CATATG	1 cut(s)	526
FblI	GTMKAC	1 cut(s)	980
Fnu4HI	GCNGC	8 cut(s)	149, 287, 335, 708, 789, 1256, 1443, 1542
FokI	GGATG	7 cut(s)	212, 332, 887, 1057, 1094, 1198, 1463
FriOI	GRGCYC	1 cut(s)	1077
Fsp4HI	GCNGC	8 cut(s)	149, 287, 335, 708, 789, 1256, 1443, 1542
FspBI	CTAG	1 cut(s)	701
GluI	GCNGC	8 cut(s)	149, 287, 335, 708, 789, 1256, 1443, 1542
GsaI	CCCAGC	2 cut(s)	344, 348
GsuI	CTGGAG	1 cut(s)	854
HaeIII	GGCC	3 cut(s)	350, 936, 991
HapII	CCGG	3 cut(s)	144, 157, 300
Hin1II	CATG	9 cut(s)	155, 237, 309, 557, 963, 1090, 1301, 1334, 1414
HincII	GTYRAC	1 cut(s)	403
HindII	GTYRAC	1 cut(s)	403
HindIII	AAGCTT	3 cut(s)	848, 915, 1401
HinfI	GANTC	8 cut(s)	11, 56, 861, 950, 1294, 1338, 1416, 1516
HpaII	CCGG	3 cut(s)	144, 157, 300
HphI	GGTGA	7 cut(s)	184, 265, 287, 430, 623, 659, 1363
Hpy166II	GTNNAC	4 cut(s)	403, 571, 981, 1378
Hpy188I	TCNGA	8 cut(s)	72, 88, 104, 512, 691, 1147, 1327, 1362
Hpy188III	TCNNGA	5 cut(s)	117, 157, 215, 477, 1042
Hpy8I	GTNNAC	4 cut(s)	403, 571, 981, 1378
HpyAV	CCTTC	5 cut(s)	181, 352, 373, 638, 808
HpyCH4III	ACNGT	7 cut(s)	21, 928, 1094, 1196, 1221, 1438, 1538
HpyCH4IV	ACGT	1 cut(s)	459
HpyF10VI	GCNNNNNNNGC	5 cut(s)	74, 292, 334, 794, 914
HpyF3I	CTNAG	3 cut(s)	368, 968, 1005
HpySE526I	ACGT	1 cut(s)	459
Hsp92II	CATG	9 cut(s)	155, 237, 309, 557, 963, 1090, 1301, 1334, 1414
Kpn2I	TCCGGA	1 cut(s)	156
Kzo9I	GATC	4 cut(s)	104, 230, 842, 1395
LmnI	GCTCC	4 cut(s)	364, 664, 1051, 1252
Lsp1109I	GCAGC	8 cut(s)	160, 298, 321, 694, 775, 1242, 1429, 1528
LweI	GCATC	6 cut(s)	190, 516, 727, 1220, 1255, 1331
MaeI	CTAG	1 cut(s)	701
MaeII	ACGT	1 cut(s)	459
MaeIII	GTNAC	3 cut(s)	275, 625, 1128
MalI	GATC	4 cut(s)	106, 232, 844, 1397
MbiI	CCGCTC	1 cut(s)	68
MboI	GATC	4 cut(s)	104, 230, 842, 1395
MboII	GAAGA	3 cut(s)	187, 892, 1511
MflI	RGATCY	1 cut(s)	104
MhlI	GDGCHC	3 cut(s)	573, 967, 1077
MlsI	TGGCCA	1 cut(s)	936
MluNI	TGGCCA	1 cut(s)	936
MlyI	GAGTC	2 cut(s)	959, 1410
MmeI	TCCRAC	1 cut(s)	636
MnlI	CCTC	7 cut(s)	447, 595, 654, 769, 829, 1093, 1342
Mox20I	TGGCCA	1 cut(s)	936
Mph1103I	ATGCAT	3 cut(s)	205, 826, 961
MroI	TCCGGA	1 cut(s)	156
MscI	TGGCCA	1 cut(s)	936
MseI	TTAA	7 cut(s)	50, 222, 330, 672, 804, 1343, 1547
Msp20I	TGGCCA	1 cut(s)	936
MspA1I	CMGCKG	2 cut(s)	344, 751
MspCI	CTTAAG	1 cut(s)	329
MspI	CCGG	3 cut(s)	144, 157, 300
MspR9I	CCNGG	4 cut(s)	145, 240, 521, 858
Mva1269I	GAATGC	1 cut(s)	826
MvaI	CCWGG	3 cut(s)	240, 521, 858
MwoI	GCNNNNNNNGC	5 cut(s)	74, 292, 334, 794, 914
NciI	CCSGG	1 cut(s)	145
NdeI	CATATG	1 cut(s)	526
NdeII	GATC	4 cut(s)	104, 230, 842, 1395
NlaIII	CATG	9 cut(s)	155, 237, 309, 557, 963, 1090, 1301, 1334, 1414
NlaIV	GGNNCC	3 cut(s)	28, 349, 1053
NmeAIII	GCCGAG	1 cut(s)	1147
NmuCI	GTSAC	2 cut(s)	275, 625
NsiI	ATGCAT	3 cut(s)	205, 826, 961
NspI	RCATGY	3 cut(s)	155, 309, 963
PciI	ACATGT	1 cut(s)	305
PctI	GAATGC	1 cut(s)	826
PfeI	GAWTC	6 cut(s)	11, 56, 861, 1294, 1338, 1516
PinAI	ACCGGT	1 cut(s)	299
PkrI	GCNGC	8 cut(s)	150, 288, 336, 709, 790, 1257, 1444, 1543
PleI	GAGTC	2 cut(s)	958, 1410
PpsI	GAGTC	2 cut(s)	958, 1410
PscI	ACATGT	1 cut(s)	305
Psp124BI	GAGCTC	1 cut(s)	1077
Psp6I	CCWGG	3 cut(s)	238, 519, 856
PspEI	GGTNACC	1 cut(s)	275
PspFI	CCCAGC	2 cut(s)	340, 344
PspGI	CCWGG	3 cut(s)	238, 519, 856
PspN4I	GGNNCC	3 cut(s)	28, 349, 1053
PspPI	GGNCC	4 cut(s)	348, 385, 395, 989
PstI	CTGCAG	2 cut(s)	499, 793
PstNI	CAGNNNCTG	3 cut(s)	797, 1247, 1442
PsuI	RGATCY	1 cut(s)	104
PvuII	CAGCTG	2 cut(s)	344, 751
RsaI	GTAC	3 cut(s)	304, 1379, 1461
RsaNI	GTAC	3 cut(s)	303, 1378, 1460
SacI	GAGCTC	1 cut(s)	1077
SaqAI	TTAA	7 cut(s)	50, 222, 330, 672, 804, 1343, 1547
SatI	GCNGC	8 cut(s)	149, 287, 335, 708, 789, 1256, 1443, 1542
Sau3AI	GATC	4 cut(s)	104, 230, 842, 1395
Sau96I	GGNCC	4 cut(s)	348, 385, 395, 989
SchI	GAGTC	2 cut(s)	959, 1410
ScrFI	CCNGG	4 cut(s)	145, 240, 521, 858
SduI	GDGCHC	3 cut(s)	573, 967, 1077
SfaNI	GCATC	6 cut(s)	190, 516, 727, 1220, 1255, 1331
SfcI	CTRYAG	2 cut(s)	495, 789
SinI	GGWCC	2 cut(s)	385, 395
SmlI	CTYRAG	3 cut(s)	329, 443, 1076
SmoI	CTYRAG	3 cut(s)	329, 443, 1076
SsiI	CCGC	1 cut(s)	66
SspMI	CTAG	1 cut(s)	701
SstI	GAGCTC	1 cut(s)	1077
StyD4I	CCNGG	4 cut(s)	143, 238, 519, 856
StyI	CCWWGG	1 cut(s)	280
TaaI	ACNGT	7 cut(s)	21, 928, 1094, 1196, 1221, 1438, 1538
TaiI	ACGT	1 cut(s)	462
TatI	WGTACW	1 cut(s)	1377
TfiI	GAWTC	6 cut(s)	11, 56, 861, 1294, 1338, 1516
Tru1I	TTAA	7 cut(s)	50, 222, 330, 672, 804, 1343, 1547
Tru9I	TTAA	7 cut(s)	50, 222, 330, 672, 804, 1343, 1547
TscAI	CASTG	3 cut(s)	598, 1130, 1443
TseFI	GTSAC	2 cut(s)	275, 625
TseI	GCWGC	8 cut(s)	148, 286, 334, 707, 788, 1255, 1442, 1541
Tsp45I	GTSAC	2 cut(s)	275, 625
TspDTI	ATGAA	4 cut(s)	455, 1103, 1347, 1508
TspGWI	ACGGA	3 cut(s)	408, 461, 1243
TspRI	CASTG	3 cut(s)	598, 1130, 1443
Vha464I	CTTAAG	1 cut(s)	329
VneI	GTGCAC	1 cut(s)	569
VpaK11BI	GGWCC	2 cut(s)	385, 395
XapI	RAATTY	5 cut(s)	80, 1009, 1037, 1112, 1355
XceI	RCATGY	3 cut(s)	155, 309, 963
XcmI	CCANNNNNNNNNTGG	1 cut(s)	358
XmiI	GTMKAC	1 cut(s)	980
XspI	CTAG	1 cut(s)	701
Zsp2I	ATGCAT	3 cut(s)	205, 826, 961

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.