RchiOBHm_Chr5g0028961

asparagine--tRNA ligase, cytoplasmic 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005074
Physical Location & Seq
Forward (+)
22737397 .. 22741324
3928 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30837

Sequence Viewer

Length: 1710 bp
ATGGCTACCGATTCGGCACAGTTAGGTTCCCAACTGGCTGAAGCAACTTTAAACGAATCTGTCTCCGCTCTGAAAGCAGAATTTTCTGACAGAGTTCCCATCAGATCCATAATATCACGAACTGATGGGGGGTCTGGGTTTGCCGGGCAGCATGTCCGGATTGGTGGTTGGGTGAAGACGGGCAGGAAGGCAGACAAGGATGCATTTGCTTTTCTGGAACTTAATGATGGATCATGCCCTGGCAACTTACAGGTGATTGTGGAAGCAGACAAGGGTGACCTTGGGCAGCTTGTGCCAACCGGTACATGTGTGGTTGTGGATGGCGTGCTTAAGCTGCCCCCAGCTGGGGCCAAGCAGAAGGTGGAGCTTAGAGTTGAGAAGGTGGTCCATTTAGGTCCAGTTGACCCTGCTAAATATCCGTTGCCCAAGACCAAGCTCACCCTTGAGTTTTTGAGGGACGTTGTTCATCTCCGTTCCAGAACTAACACCATCTCTGCAGTTGCTCGCATCAGAAATGCCCTGGCATATGCAACTCATACATTTTTCCAAAAGCATGGCTTCCTTTATGTGCACACTCCAATTATCACTACCAGTGATTGTGAGGGTGCTGGTGAAATGTTCCAAGTCACAACATTGATTAGCGAAGGTGAAAGGTTGGAGAGGGAGCTGATTAAGAACCCTGCCCCATCTGAAGCAGACCTAGAAGCTGCCAAGCTAATCATCAAGGAAAAAGGAGATGCTGTTTCACAGCTGAAATCTGCTAAAGCAAGTAAGGAGGAGATTGGTGCTGCAGTCGCTGAACTTAAAAGGGCGAAGGAGAATGCATTGAAGCTGGAGGAGAGATCAAAGCTTCAGCCAGGAATCCCCAAAAAGGATGGGAAGATTGACTATACACAAGATTTCTTTGCCCGTCAAGCTTTTTTGACCGTTTCTGGCCAACTCCAAGTGGAGTCTTATGCATGTGCTCTTAGTAGTGTGTATACATTTGGGCCTACTTTTCGTGCTGAGAATTCACACACTTCACGGCATTTGGCAGAATTCTGGATGGTGGAGCCTGAATTAGCGTTTGCAGAGCTCAAGGATGACATGAACTGTGCAGAGGCGTATGTGAAATTCTTGTGTCAGTGGTTACTTGACAACTGCTCTGACGATATGGAGTTTATTTCTCGGCAATTTGATAAAACTTGCATAGACCGTCTAAAGATGGTTGCATCCACACCGTTTGAACGGATTACATATACAGAAGCTGTGGAGCTGCTAATTGATGCTGTGAAAAATGGCAAGAAGTTTGAGAATCATGTAGAGTGGGGGATTGACTTGGCATCTGAACATGAAAGATTCTTAACAGAGGTGAAATTTCAGAAGCCTGTTATTGTGTACAATTACCCAAAAGGGATCAAAGCTTTCTACATGAGACTCAATGATGATAACAAAACAGTGGCTGCTATGGATGTCCTTGTACCAAAGGTGGGAGAGTTGATTGGGGGTAGCCAAAGGGAAGAACGCTATGATGTGATTCATAGCAGGATTGCAGAGATGGGTCTGCCTATTGAGCCGTACGAGTGGTATCTTGACTTGCGCCGCTTTGGAACTGTCAAACATGCTGGTTTTGGTTTAGGGTTTGAACGGATGCTTTTGTTTGCCACAGGCCTTGACAATATCAGAGATGTTATTCCATTCCCTAGATATCCTGGAAGAGCAGATCTTTAA

Protein Analysis

569

Amino Acids

63.42

Weight (kDa)

6.04

Isoelectric Point (pI)

30.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_anti-codon PF01336 52 - 129 2e-08 OB-fold nucleic acid binding domain
tRNA-synt_2 PF00152 155 - 212 3.9e-07 tRNA synthetases class II (D, K and N)
WHEP-TRS PF00458 241 - 274 1.2e-06 WHEP-TRS domain
tRNA-synt_2 PF00152 300 - 562 1.2e-50 tRNA synthetases class II (D, K and N)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 68
AccI GTMKAC 1 cut(s) 980
AccIII TCCGGA 1 cut(s) 156
AciI CCGC 2 cut(s) 66, 1582
AclWI GGATC 3 cut(s) 99, 238, 1403
AcoI YGGCCR 1 cut(s) 934
AcsI RAATTY 5 cut(s) 80, 1009, 1037, 1112, 1355
AcuI CTGAAG 3 cut(s) 60, 711, 836
AfaI GTAC 4 cut(s) 304, 1379, 1461, 1559
AfiI CCNNNNNNNGG 5 cut(s) 344, 345, 346, 871, 1469
AflII CTTAAG 1 cut(s) 329
AflIII ACRYGT 1 cut(s) 305
AgeI ACCGGT 1 cut(s) 299
AgsI TTSAA 3 cut(s) 829, 1226, 1625
AjnI CCWGG 4 cut(s) 238, 519, 856, 1690
Alw21I GWGCWC 3 cut(s) 573, 967, 1077
Alw26I GTCTC 2 cut(s) 67, 1408
Alw44I GTGCAC 1 cut(s) 569
AlwI GGATC 3 cut(s) 99, 238, 1403
AlwNI CAGNNNCTG 3 cut(s) 797, 1247, 1442
Aor13HI TCCGGA 1 cut(s) 156
AoxI GGCC 4 cut(s) 348, 934, 989, 1648
ApaLI GTGCAC 1 cut(s) 569
ApeKI GCWGC 7 cut(s) 148, 286, 334, 707, 788, 1255, 1442
ApoI RAATTY 5 cut(s) 80, 1009, 1037, 1112, 1355
AsiGI ACCGGT 1 cut(s) 299
AspLEI GCGC 1 cut(s) 1581
AspS9I GGNCC 4 cut(s) 348, 385, 395, 989
AsuC2I CCSGG 1 cut(s) 145
AsuHPI GGTGA 7 cut(s) 184, 265, 287, 430, 623, 659, 1363
AvaII GGWCC 2 cut(s) 385, 395
BaeGI GKGCMC 1 cut(s) 573
BaeI ACNNNNGTAYC 2 cut(s) 294, 327
BalI TGGCCA 1 cut(s) 936
BanII GRGCYC 1 cut(s) 1077
BbsI GAAGAC 1 cut(s) 182
Bbv12I GWGCWC 3 cut(s) 573, 967, 1077
BbvI GCAGC 7 cut(s) 160, 298, 321, 694, 775, 1242, 1429
BceAI ACGGC 2 cut(s) 1040, 1540
BciT130I CCWGG 4 cut(s) 240, 521, 858, 1692
BcnI CCSGG 1 cut(s) 145
BcoDI GTCTC 2 cut(s) 67, 1408
BfaI CTAG 2 cut(s) 701, 1683
BfmI CTRYAG 2 cut(s) 495, 789
BfrI CTTAAG 1 cut(s) 329
BglII AGATCT 1 cut(s) 1702
BisI GCNGC 8 cut(s) 149, 287, 335, 708, 789, 1256, 1443, 1582
BlsI GCNGC 8 cut(s) 150, 288, 336, 709, 790, 1257, 1444, 1583
Bme1390I CCNGG 5 cut(s) 145, 240, 521, 858, 1692
Bme18I GGWCC 2 cut(s) 385, 395
BmgT120I GGNCC 4 cut(s) 348, 385, 395, 989
BmiI GGNNCC 3 cut(s) 28, 349, 1053
BmrFI CCNGG 5 cut(s) 145, 240, 521, 858, 1692
BmsI GCATC 7 cut(s) 190, 516, 727, 1220, 1255, 1331, 1620
BpiI GAAGAC 1 cut(s) 182
BpmI CTGGAG 1 cut(s) 854
BpuEI CTTGAG 2 cut(s) 464, 1061
BpuMI CCSGG 1 cut(s) 145
BsaJI CCNNGG 3 cut(s) 238, 280, 519
BsaWI WCCGGW 2 cut(s) 156, 299
BsaXI ACNNNNNCTCC 2 cut(s) 726, 756
Bsc4I CCNNNNNNNGG 5 cut(s) 344, 345, 346, 871, 1469
Bse118I RCCGGY 1 cut(s) 299
Bse1I ACTGG 3 cut(s) 39, 398, 591
BseAI TCCGGA 1 cut(s) 156
BseBI CCWGG 4 cut(s) 240, 521, 858, 1692
BseDI CCNNGG 3 cut(s) 238, 280, 519
BseGI GGATG 8 cut(s) 205, 325, 880, 1050, 1087, 1211, 1456, 1635
BseLI CCNNNNNNNGG 5 cut(s) 344, 345, 346, 871, 1469
BseMII CTCAG 1 cut(s) 996
BseNI ACTGG 3 cut(s) 39, 398, 591
BseRI GAGGAG 2 cut(s) 791, 851
BseSI GKGCMC 1 cut(s) 573
BseXI GCAGC 7 cut(s) 160, 298, 321, 694, 775, 1242, 1429
BseYI CCCAGC 2 cut(s) 340, 344
BsgI GTGCAG 1 cut(s) 1116
BshFI GGCC 4 cut(s) 350, 936, 991, 1650
BshTI ACCGGT 1 cut(s) 299
BsiHKAI GWGCWC 3 cut(s) 573, 967, 1077
BsiSI CCGG 3 cut(s) 144, 157, 300
BsiWI CGTACG 1 cut(s) 1557
BslFI GGGAC 1 cut(s) 470
BslI CCNNNNNNNGG 5 cut(s) 344, 345, 346, 871, 1469
BsmAI GTCTC 2 cut(s) 67, 1408
BsmFI GGGAC 1 cut(s) 470
BsmI GAATGC 1 cut(s) 826
BsnI GGCC 4 cut(s) 350, 936, 991, 1650
Bsp1286I GDGCHC 3 cut(s) 573, 967, 1077
Bsp13I TCCGGA 1 cut(s) 156
Bsp1407I TGTACA 1 cut(s) 1377
Bsp143I GATC 5 cut(s) 104, 230, 842, 1395, 1702
BspACI CCGC 2 cut(s) 66, 1582
BspANI GGCC 4 cut(s) 350, 936, 991, 1650
BspCNI CTCAG 1 cut(s) 997
BspEI TCCGGA 1 cut(s) 156
BspLI GGNNCC 3 cut(s) 28, 349, 1053
BspMAI CTGCAG 2 cut(s) 499, 793
BspPI GGATC 3 cut(s) 99, 238, 1403
BspQI GCTCTTC 1 cut(s) 1690
BspTI CTTAAG 1 cut(s) 329
BsrBI CCGCTC 1 cut(s) 68
BsrFI RCCGGY 1 cut(s) 299
BsrGI TGTACA 1 cut(s) 1377
BsrI ACTGG 3 cut(s) 39, 398, 591
BssAI RCCGGY 1 cut(s) 299
BssECI CCNNGG 3 cut(s) 238, 280, 519
BssMI GATC 5 cut(s) 104, 230, 842, 1395, 1702
BssNAI GTATAC 1 cut(s) 981
BssT1I CCWWGG 1 cut(s) 280
Bst1107I GTATAC 1 cut(s) 981
Bst2UI CCWGG 4 cut(s) 240, 521, 858, 1692
Bst4CI ACNGT 7 cut(s) 21, 928, 1094, 1196, 1221, 1438, 1594
Bst6I CTCTTC 1 cut(s) 1690
BstAFI CTTAAG 1 cut(s) 329
BstAPI GCANNNNNTGC 1 cut(s) 292
BstAUI TGTACA 1 cut(s) 1377
BstC8I GCNNGC 2 cut(s) 326, 505
BstDEI CTNAG 3 cut(s) 368, 968, 1005
BstEII GGTNACC 1 cut(s) 275
BstF5I GGATG 8 cut(s) 205, 325, 880, 1050, 1087, 1211, 1456, 1635
BstHHI GCGC 1 cut(s) 1581
BstKTI GATC 5 cut(s) 107, 233, 845, 1398, 1705
BstMAI GTCTC 2 cut(s) 67, 1408
BstMBI GATC 5 cut(s) 104, 230, 842, 1395, 1702
BstMWI GCNNNNNNNGC 6 cut(s) 74, 292, 334, 794, 914, 1552
BstNI CCWGG 4 cut(s) 240, 521, 858, 1692
BstNSI RCATGY 4 cut(s) 155, 309, 963, 1604
BstPI GGTNACC 1 cut(s) 275
BstSCI CCNGG 5 cut(s) 143, 238, 519, 856, 1690
BstSFI CTRYAG 2 cut(s) 495, 789
BstSLI GKGCMC 1 cut(s) 573
BstV1I GCAGC 7 cut(s) 160, 298, 321, 694, 775, 1242, 1429
BstV2I GAAGAC 1 cut(s) 182
BstX2I RGATCY 2 cut(s) 104, 1702
BstXI CCANNNNNNTGG 1 cut(s) 554
BstYI RGATCY 2 cut(s) 104, 1702
BstZ17I GTATAC 1 cut(s) 981
BsuRI GGCC 4 cut(s) 350, 936, 991, 1650
BtsCI GGATG 8 cut(s) 205, 325, 880, 1050, 1087, 1211, 1456, 1635
BtsIMutI CAGTG 3 cut(s) 598, 1130, 1443
Cac8I GCNNGC 2 cut(s) 326, 505
CaiI CAGNNNCTG 3 cut(s) 797, 1247, 1442
CfoI GCGC 1 cut(s) 1581
Cfr10I RCCGGY 1 cut(s) 299
Cfr13I GGNCC 4 cut(s) 348, 385, 395, 989
Csp6I GTAC 4 cut(s) 303, 1378, 1460, 1558
CspAI ACCGGT 1 cut(s) 299
CspCI CAANNNNNGTGG 4 cut(s) 1204, 1239, 1633, 1668
CviQI GTAC 4 cut(s) 303, 1378, 1460, 1558
DdeI CTNAG 3 cut(s) 368, 968, 1005
DpnI GATC 5 cut(s) 106, 232, 844, 1397, 1704
DpnII GATC 5 cut(s) 104, 230, 842, 1395, 1702
DraI TTTAAA 1 cut(s) 51
EaeI YGGCCR 1 cut(s) 934
Eam1104I CTCTTC 1 cut(s) 1690
EarI CTCTTC 1 cut(s) 1690
Ecl136II GAGCTC 1 cut(s) 1075
Eco130I CCWWGG 1 cut(s) 280
Eco147I AGGCCT 1 cut(s) 1650
Eco24I GRGCYC 1 cut(s) 1077
Eco32I GATATC 1 cut(s) 1688
Eco47I GGWCC 2 cut(s) 385, 395
Eco53kI GAGCTC 1 cut(s) 1075
Eco57I CTGAAG 3 cut(s) 60, 711, 836
Eco91I GGTNACC 1 cut(s) 275
EcoICRI GAGCTC 1 cut(s) 1075
EcoO65I GGTNACC 1 cut(s) 275
EcoRI GAATTC 2 cut(s) 1009, 1037
EcoRII CCWGG 4 cut(s) 238, 519, 856, 1690
EcoRV GATATC 1 cut(s) 1688
EcoT14I CCWWGG 1 cut(s) 280
EcoT22I ATGCAT 3 cut(s) 205, 826, 961
EcoT38I GRGCYC 1 cut(s) 1077
ErhI CCWWGG 1 cut(s) 280
FalI AAGNNNNNCTT 2 cut(s) 542, 574
FaqI GGGAC 1 cut(s) 470
FauNDI CATATG 1 cut(s) 526
FblI GTMKAC 1 cut(s) 980
Fnu4HI GCNGC 8 cut(s) 149, 287, 335, 708, 789, 1256, 1443, 1582
FokI GGATG 8 cut(s) 212, 332, 887, 1057, 1094, 1198, 1463, 1642
FriOI GRGCYC 1 cut(s) 1077
Fsp4HI GCNGC 8 cut(s) 149, 287, 335, 708, 789, 1256, 1443, 1582
FspBI CTAG 2 cut(s) 701, 1683
GlaI GCGC 1 cut(s) 1580
GluI GCNGC 8 cut(s) 149, 287, 335, 708, 789, 1256, 1443, 1582
GsaI CCCAGC 2 cut(s) 344, 348
GsuI CTGGAG 1 cut(s) 854
HaeIII GGCC 4 cut(s) 350, 936, 991, 1650
HapII CCGG 3 cut(s) 144, 157, 300
HhaI GCGC 1 cut(s) 1581
Hin6I GCGC 1 cut(s) 1579
HinP1I GCGC 1 cut(s) 1579
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HindIII AAGCTT 3 cut(s) 848, 915, 1401
HinfI GANTC 8 cut(s) 11, 56, 861, 950, 1294, 1338, 1416, 1516
HpaII CCGG 3 cut(s) 144, 157, 300
HphI GGTGA 7 cut(s) 184, 265, 287, 430, 623, 659, 1363
Hpy166II GTNNAC 4 cut(s) 403, 571, 981, 1378
Hpy188I TCNGA 9 cut(s) 72, 88, 104, 512, 691, 1147, 1327, 1362, 1664
Hpy188III TCNNGA 6 cut(s) 117, 157, 215, 477, 1042, 1571
Hpy8I GTNNAC 4 cut(s) 403, 571, 981, 1378
HpyAV CCTTC 5 cut(s) 181, 352, 373, 638, 808
HpyCH4III ACNGT 7 cut(s) 21, 928, 1094, 1196, 1221, 1438, 1594
HpyCH4IV ACGT 1 cut(s) 459
HpyF10VI GCNNNNNNNGC 6 cut(s) 74, 292, 334, 794, 914, 1552
HpyF3I CTNAG 3 cut(s) 368, 968, 1005
HpySE526I ACGT 1 cut(s) 459
HspAI GCGC 1 cut(s) 1579
Kpn2I TCCGGA 1 cut(s) 156
Kzo9I GATC 5 cut(s) 104, 230, 842, 1395, 1702
LguI GCTCTTC 1 cut(s) 1690
LmnI GCTCC 4 cut(s) 364, 664, 1051, 1252
Lsp1109I GCAGC 7 cut(s) 160, 298, 321, 694, 775, 1242, 1429
LweI GCATC 7 cut(s) 190, 516, 727, 1220, 1255, 1331, 1620
MaeI CTAG 2 cut(s) 701, 1683
MaeII ACGT 1 cut(s) 459
MaeIII GTNAC 3 cut(s) 275, 625, 1128
MalI GATC 5 cut(s) 106, 232, 844, 1397, 1704
MbiI CCGCTC 1 cut(s) 68
MboI GATC 5 cut(s) 104, 230, 842, 1395, 1702
MboII GAAGA 4 cut(s) 187, 892, 1511, 1707
MflI RGATCY 2 cut(s) 104, 1702
MhlI GDGCHC 3 cut(s) 573, 967, 1077
MlsI TGGCCA 1 cut(s) 936
MluNI TGGCCA 1 cut(s) 936
MlyI GAGTC 2 cut(s) 959, 1410
MmeI TCCRAC 1 cut(s) 636
MnlI CCTC 7 cut(s) 447, 595, 654, 769, 829, 1093, 1342
Mox20I TGGCCA 1 cut(s) 936
Mph1103I ATGCAT 3 cut(s) 205, 826, 961
MroI TCCGGA 1 cut(s) 156
MscI TGGCCA 1 cut(s) 936
MseI TTAA 7 cut(s) 50, 222, 330, 672, 804, 1343, 1708
Msp20I TGGCCA 1 cut(s) 936
MspA1I CMGCKG 2 cut(s) 344, 751
MspCI CTTAAG 1 cut(s) 329
MspI CCGG 3 cut(s) 144, 157, 300
MspR9I CCNGG 5 cut(s) 145, 240, 521, 858, 1692
Mva1269I GAATGC 1 cut(s) 826
MvaI CCWGG 4 cut(s) 240, 521, 858, 1692
MwoI GCNNNNNNNGC 6 cut(s) 74, 292, 334, 794, 914, 1552
NciI CCSGG 1 cut(s) 145
NdeI CATATG 1 cut(s) 526
NdeII GATC 5 cut(s) 104, 230, 842, 1395, 1702
NlaIV GGNNCC 3 cut(s) 28, 349, 1053
NmeAIII GCCGAG 1 cut(s) 1147
NmuCI GTSAC 2 cut(s) 275, 625
NsiI ATGCAT 3 cut(s) 205, 826, 961
NspI RCATGY 4 cut(s) 155, 309, 963, 1604
PceI AGGCCT 1 cut(s) 1650
PciI ACATGT 1 cut(s) 305
PciSI GCTCTTC 1 cut(s) 1690
PctI GAATGC 1 cut(s) 826
PfeI GAWTC 6 cut(s) 11, 56, 861, 1294, 1338, 1516
Pfl23II CGTACG 1 cut(s) 1557
PfoI TCCNGGA 1 cut(s) 1690
PinAI ACCGGT 1 cut(s) 299
PkrI GCNGC 8 cut(s) 150, 288, 336, 709, 790, 1257, 1444, 1583
PleI GAGTC 2 cut(s) 958, 1410
PpsI GAGTC 2 cut(s) 958, 1410
PscI ACATGT 1 cut(s) 305
Psp124BI GAGCTC 1 cut(s) 1077
Psp6I CCWGG 4 cut(s) 238, 519, 856, 1690
PspEI GGTNACC 1 cut(s) 275
PspFI CCCAGC 2 cut(s) 340, 344
PspGI CCWGG 4 cut(s) 238, 519, 856, 1690
PspLI CGTACG 1 cut(s) 1557
PspN4I GGNNCC 3 cut(s) 28, 349, 1053
PspPI GGNCC 4 cut(s) 348, 385, 395, 989
PstI CTGCAG 2 cut(s) 499, 793
PstNI CAGNNNCTG 3 cut(s) 797, 1247, 1442
PsuI RGATCY 2 cut(s) 104, 1702
PvuII CAGCTG 2 cut(s) 344, 751
RsaI GTAC 4 cut(s) 304, 1379, 1461, 1559
RsaNI GTAC 4 cut(s) 303, 1378, 1460, 1558
SacI GAGCTC 1 cut(s) 1077
SapI GCTCTTC 1 cut(s) 1690
SaqAI TTAA 7 cut(s) 50, 222, 330, 672, 804, 1343, 1708
SatI GCNGC 8 cut(s) 149, 287, 335, 708, 789, 1256, 1443, 1582
Sau3AI GATC 5 cut(s) 104, 230, 842, 1395, 1702
Sau96I GGNCC 4 cut(s) 348, 385, 395, 989
SchI GAGTC 2 cut(s) 959, 1410
ScrFI CCNGG 5 cut(s) 145, 240, 521, 858, 1692
SduI GDGCHC 3 cut(s) 573, 967, 1077
SfaNI GCATC 7 cut(s) 190, 516, 727, 1220, 1255, 1331, 1620
SfcI CTRYAG 2 cut(s) 495, 789
SinI GGWCC 2 cut(s) 385, 395
SmlI CTYRAG 3 cut(s) 329, 443, 1076
SmoI CTYRAG 3 cut(s) 329, 443, 1076
SseBI AGGCCT 1 cut(s) 1650
SsiI CCGC 2 cut(s) 66, 1582
SspMI CTAG 2 cut(s) 701, 1683
SstI GAGCTC 1 cut(s) 1077
StuI AGGCCT 1 cut(s) 1650
StyD4I CCNGG 5 cut(s) 143, 238, 519, 856, 1690
StyI CCWWGG 1 cut(s) 280
TaaI ACNGT 7 cut(s) 21, 928, 1094, 1196, 1221, 1438, 1594
TaiI ACGT 1 cut(s) 462
TatI WGTACW 1 cut(s) 1377
TauI GCSGC 1 cut(s) 1584
TfiI GAWTC 6 cut(s) 11, 56, 861, 1294, 1338, 1516
Tru1I TTAA 7 cut(s) 50, 222, 330, 672, 804, 1343, 1708
Tru9I TTAA 7 cut(s) 50, 222, 330, 672, 804, 1343, 1708
TscAI CASTG 3 cut(s) 598, 1130, 1443
TseFI GTSAC 2 cut(s) 275, 625
TseI GCWGC 7 cut(s) 148, 286, 334, 707, 788, 1255, 1442
Tsp45I GTSAC 2 cut(s) 275, 625
TspDTI ATGAA 4 cut(s) 455, 1103, 1347, 1508
TspGWI ACGGA 4 cut(s) 408, 461, 1243, 1642
TspRI CASTG 3 cut(s) 598, 1130, 1443
Vha464I CTTAAG 1 cut(s) 329
VneI GTGCAC 1 cut(s) 569
VpaK11BI GGWCC 2 cut(s) 385, 395
XapI RAATTY 5 cut(s) 80, 1009, 1037, 1112, 1355
XceI RCATGY 4 cut(s) 155, 309, 963, 1604
XcmI CCANNNNNNNNNTGG 1 cut(s) 358
XmiI GTMKAC 1 cut(s) 980
XspI CTAG 2 cut(s) 701, 1683
Zsp2I ATGCAT 3 cut(s) 205, 826, 961
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.