Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0005074

Physical Location & Seq

Genomic Coordinates Reverse (-)

33937137 .. 33941844

Gene Structure 4708 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1251800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1710 bp

ATGGCGGATGATTCCACGCCTCCAAGTGCCCAGCTTGCTGCAGTAACCTTAAGCAATGATGCCTCCGTTCTAAAAGCAGAGTTTTCTGATAGAGTCCTCATCCGTTCCATTATTTCGCGACCTGATGGAGGATCTGGGTTGGCTGGGCAGAAAATTCGGATTGGTGGGTGGGTGAAGACTGGTCGCAAGGCAGACAAGGATGCATTTGCATTCCTGGAGCTGAATGACGGGTCATGCGCCAGCAACCTTCAGGTGATTGTGAAGGCTGACAAGTATGACCTTGGGCAGCTTGTGCAGACCGGTACTTGCCTGTCTGTGGATGGTGTGCTTAAGCTTCCCCCAGATGGGAAAAAGCAGAAGGTGGAGCTTGATGTTGAGAAGGTGATCCATGTGGGTCCGGTTGACCCAGCAAAATACCCGTTGCCCAAGACCAGGCTCACCCTCGAGTTTTTGAGGGAGCATGTTCATTTGCGTTCCAGAACCAACACTATCTCTGCAGTTACCCGAATCAGAAATGCATTGGCATTTGCAACCCACTCATTTTTCCATAAGCATGGATTTCTTTATGTGCAAACTCCAATTATCACCACCAGTGACTGTGAGGGTGCTGGTGAGATGTTTCAAGTCACAACCTTGTTTAGTGAAGCTGAAAAGTTGGAAAAGGATCTGATTGAGAACCCTCCTCCATCAGAAGCAGACATAGAAGCTGCCAAGCTTATTGTCAAGGAGAGAGGAGATGATGTTGCTCAGCTGAAATCTGCTAAAGCAAGTAAGCAGGAAATTGGTGCTGCTGTGGCTGAGCTTAAAAAGGCAAAGGATAATGTCTTAAAGCTGGAGGAGCGATCTAAGCTTAAACCTGGTATCCCTCGAAAGGATGGGAAGGTTGATTATACCCAAGATTTCTTTGCCCGTCAAGCCTTTTTGACTGTTTCTGGCCAATTACAAGTCGAATCTTATGCGTGTGCTCTTAGTAGCGTCTATACATTTGGGCCTACTTTTCGAGCTGAGAACTCCCATACTTCAAGGCATTTGGCAGAGTTCTGGATGGTGGAGCCAGAAATTGCATTTGCAGAACTTGAGGATGATATGAACTGTGCAGAGGCGTATGTGAAATTTCTGTGTCAGTGGTTACTTGACGACTGCAGGGAAGACATGGAATTTATGGCTGACAAAATTGATAAAACTTGCATTGATCGTCTAACAATGGTTGCTAAGACACCCTTTAAACGGATTACCTATACAGAAGCAGTAGATCTGCTAATTGATGCTGTGAAAAAGGGCAAGAAGTTTGAGAACCATGTAGAGTGGGGGATTGATTTGGCATCTGAGCACGAAAGATACCTGACAGAAGTATTATTTCAAAAACCTGTTATCGTTTACAACTATCCTAAAGGGATCAAAGCATTCTACATGAGGCTCAATGATGATTTAAAAACAGTGGCTGCTATGGATGTTTTGGTACCAAAGGTGGGAGAGTTGATTGGGGGAAGCCAAAGGGAAGAGCGTTACGATATTATTATGAGCAGGATTGAGGAAATGGGTCTGCCCGCTGAGCCATACGAGTGGTACCTTGACTTGCGGCGTTTTGGGACTGTCAAACATTGTGGTTTTGGGTTAGGATTCGAACGAATGGTCCTCTTTGCTACCGGGATTGACAACATCAGAGATGTTATTCCCTTCCCTAGATATCCAGGAAGAGCAGATCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0007275 GO:0008150 GO:0009507 GO:0009536 GO:0009790 GO:0009791 GO:0009793 GO:0010035 GO:0010038 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

WHEP-TRS tRNA-synt_2 tRNA_anti-codon

Protein Analysis

570

Amino Acids

63.76

Weight (kDa)

5.81

Isoelectric Point (pI)

29.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA_anti-codon	PF01336	53 - 128	2.3e-07	OB-fold nucleic acid binding domain
tRNA-synt_2	PF00152	152 - 211	4.9e-07	tRNA synthetases class II (D, K and N)
tRNA-synt_2	PF00152	300 - 562	2.3e-50	tRNA synthetases class II (D, K and N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005074)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70980 AT5G56680
fragaria_vesca	FvH4_3g17430
malus_domestica	MD01G1115900.v1.1 MD03G1251800.v1.1 MD05G1158600.v1.1 MD11G1273000.v1.1
prunus_persica	Prupe.4G154500_v2.0.a1 Prupe.4G154500_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1
pyrus_communis	pycom03g19910 pycom05g15060 pycom11g24090
rosa_chinensis	RchiOBHm_Chr5g0028961
rosa_laevigata	RLG00000033131
rosa_multiflora	Rmu_co7984584.1_g000001 Rmu_sc0009042.1_g000005
rosa_roxburghii	Rroxscaffold_1G00050930
rosa_rugosa	Rorug05G0111900 Rorug05G0112000 Rorug05G0112000
rosa_samantha	Rh5AG204000 Rh5BG202300 Rh5CG223100 Rh5DG206100
rosa_wichuraiana	Rw5G018490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	2 cut(s)	1459, 1566
AccB1I	GGYRCC	2 cut(s)	1459, 1566
AccII	CGCG	1 cut(s)	118
AciI	CCGC	3 cut(s)	5, 1548, 1579
AclWI	GGATC	4 cut(s)	139, 379, 672, 1403
AcoI	YGGCCR	1 cut(s)	934
AcsI	RAATTY	3 cut(s)	153, 1112, 1157
AcuI	CTGAAG	1 cut(s)	233
AfaI	GTAC	3 cut(s)	304, 1461, 1568
AfiI	CCNNNNNNNGG	8 cut(s)	128, 316, 344, 345, 432, 871, 1227, 1469
AflII	CTTAAG	2 cut(s)	49, 329
AgeI	ACCGGT	1 cut(s)	299
AgsI	TTSAA	3 cut(s)	623, 1023, 1361
AjnI	CCWGG	4 cut(s)	213, 431, 856, 1690
AloI	GAACNNNNNNTCC	2 cut(s)	1617, 1649
Alw21I	GWGCWC	2 cut(s)	967, 1332
AlwI	GGATC	4 cut(s)	139, 379, 672, 1403
AlwNI	CAGNNNCTG	2 cut(s)	597, 1442
Ama87I	CYCGRG	1 cut(s)	443
AoxI	GGCC	2 cut(s)	934, 989
ApeKI	GCWGC	5 cut(s)	38, 286, 707, 788, 1442
ApoI	RAATTY	3 cut(s)	153, 1112, 1157
ArsI	GACNNNNNNTTYG	2 cut(s)	1617, 1649
AsiGI	ACCGGT	1 cut(s)	299
Asp718I	GGTACC	2 cut(s)	1459, 1566
AspLEI	GCGC	1 cut(s)	239
AspS9I	GGNCC	3 cut(s)	395, 989, 1633
AsuC2I	CCSGG	1 cut(s)	1648
AsuHPI	GGTGA	6 cut(s)	184, 265, 394, 430, 577, 623
AsuII	TTCGAA	1 cut(s)	1623
AvaI	CYCGRG	1 cut(s)	443
AvaII	GGWCC	2 cut(s)	395, 1633
BaeGI	GKGCMC	1 cut(s)	31
BalI	TGGCCA	1 cut(s)	936
BanI	GGYRCC	2 cut(s)	1459, 1566
BbsI	GAAGAC	2 cut(s)	182, 1155
Bbv12I	GWGCWC	2 cut(s)	967, 1332
BbvI	GCAGC	5 cut(s)	25, 298, 694, 775, 1429
BccI	CCATC	6 cut(s)	119, 314, 338, 694, 869, 1039
BcgI	CGANNNNNNTGC	4 cut(s)	137, 171, 938, 972
BciT130I	CCWGG	4 cut(s)	215, 433, 858, 1692
BciVI	GTATCC	1 cut(s)	872
BcnI	CCSGG	1 cut(s)	1648
BfaI	CTAG	1 cut(s)	1683
BfmI	CTRYAG	3 cut(s)	39, 495, 1141
BfrI	CTTAAG	2 cut(s)	49, 329
BfuI	GTATCC	1 cut(s)	872
BglII	AGATCT	2 cut(s)	1252, 1702
BisI	GCNGC	6 cut(s)	39, 287, 708, 789, 1443, 1580
BlpI	GCTNAGC	3 cut(s)	747, 798, 1551
BlsI	GCNGC	6 cut(s)	40, 288, 709, 790, 1444, 1581
Bme1390I	CCNGG	5 cut(s)	215, 433, 858, 1648, 1692
Bme18I	GGWCC	2 cut(s)	395, 1633
BmeT110I	CYCGRG	1 cut(s)	443
BmgT120I	GGNCC	3 cut(s)	395, 989, 1633
BmiI	GGNNCC	4 cut(s)	396, 1053, 1461, 1568
BmrFI	CCNGG	5 cut(s)	215, 433, 858, 1648, 1692
BmsI	GCATC	4 cut(s)	49, 190, 1255, 1331
BpiI	GAAGAC	2 cut(s)	182, 1155
BpmI	CTGGAG	2 cut(s)	236, 854
Bpu1102I	GCTNAGC	3 cut(s)	747, 798, 1551
Bpu14I	TTCGAA	1 cut(s)	1623
BpuEI	CTTGAG	1 cut(s)	1097
BpuMI	CCSGG	1 cut(s)	1648
BsaJI	CCNNGG	1 cut(s)	280
BsaWI	WCCGGW	2 cut(s)	299, 397
BsaXI	ACNNNNNCTCC	2 cut(s)	726, 756
Bsc4I	CCNNNNNNNGG	8 cut(s)	128, 316, 344, 345, 432, 871, 1227, 1469
Bse118I	RCCGGY	1 cut(s)	299
Bse1I	ACTGG	2 cut(s)	184, 591
Bse3DI	GCAATG	1 cut(s)	61
BseBI	CCWGG	4 cut(s)	215, 433, 858, 1692
BseDI	CCNNGG	1 cut(s)	280
BseGI	GGATG	8 cut(s)	13, 99, 205, 325, 880, 1050, 1087, 1456
BseLI	CCNNNNNNNGG	8 cut(s)	128, 316, 344, 345, 432, 871, 1227, 1469
BseMI	GCAATG	1 cut(s)	61
BseMII	CTCAG	5 cut(s)	761, 789, 996, 1317, 1542
BseNI	ACTGG	2 cut(s)	184, 591
BseRI	GAGGAG	3 cut(s)	672, 747, 851
BseSI	GKGCMC	1 cut(s)	31
BseXI	GCAGC	5 cut(s)	25, 298, 694, 775, 1429
BseYI	CCCAGC	3 cut(s)	30, 143, 406
BsgI	GTGCAG	2 cut(s)	314, 1116
Bsh1236I	CGCG	1 cut(s)	118
BshFI	GGCC	2 cut(s)	936, 991
BshNI	GGYRCC	2 cut(s)	1459, 1566
BshTI	ACCGGT	1 cut(s)	299
BsiHKAI	GWGCWC	2 cut(s)	967, 1332
BsiHKCI	CYCGRG	1 cut(s)	443
BsiSI	CCGG	3 cut(s)	300, 398, 1647
BslFI	GGGAC	1 cut(s)	1603
BslI	CCNNNNNNNGG	8 cut(s)	128, 316, 344, 345, 432, 871, 1227, 1469
BsmFI	GGGAC	1 cut(s)	1603
BsmI	GAATGC	2 cut(s)	209, 1403
BsnI	GGCC	2 cut(s)	936, 991
BsoBI	CYCGRG	1 cut(s)	443
Bsp119I	TTCGAA	1 cut(s)	1623
Bsp1286I	GDGCHC	3 cut(s)	31, 967, 1332
Bsp143I	GATC	8 cut(s)	131, 384, 664, 842, 1192, 1252, 1395, 1702
Bsp1720I	GCTNAGC	3 cut(s)	747, 798, 1551
Bsp68I	TCGCGA	1 cut(s)	118
BspACI	CCGC	3 cut(s)	5, 1548, 1579
BspANI	GGCC	2 cut(s)	936, 991
BspCNI	CTCAG	5 cut(s)	760, 790, 997, 1318, 1543
BspFNI	CGCG	1 cut(s)	118
BspLI	GGNNCC	4 cut(s)	396, 1053, 1461, 1568
BspMAI	CTGCAG	3 cut(s)	43, 499, 1145
BspPI	GGATC	4 cut(s)	139, 379, 672, 1403
BspQI	GCTCTTC	2 cut(s)	1494, 1690
BspT104I	TTCGAA	1 cut(s)	1623
BspT107I	GGYRCC	2 cut(s)	1459, 1566
BspTI	CTTAAG	2 cut(s)	49, 329
BsrDI	GCAATG	1 cut(s)	61
BsrFI	RCCGGY	1 cut(s)	299
BsrI	ACTGG	2 cut(s)	184, 591
BssAI	RCCGGY	1 cut(s)	299
BssECI	CCNNGG	1 cut(s)	280
BssMI	GATC	8 cut(s)	131, 384, 664, 842, 1192, 1252, 1395, 1702
BssT1I	CCWWGG	1 cut(s)	280
Bst2UI	CCWGG	4 cut(s)	215, 433, 858, 1692
Bst4CI	ACNGT	5 cut(s)	599, 928, 1094, 1438, 1594
Bst6I	CTCTTC	2 cut(s)	1494, 1690
BstAFI	CTTAAG	2 cut(s)	49, 329
BstAPI	GCANNNNNTGC	1 cut(s)	292
BstBI	TTCGAA	1 cut(s)	1623
BstC8I	GCNNGC	3 cut(s)	36, 241, 1548
BstDEI	CTNAG	8 cut(s)	747, 798, 846, 968, 1005, 1212, 1326, 1551
BstENI	CCTNNNNNAGG	1 cut(s)	126
BstF5I	GGATG	8 cut(s)	13, 99, 205, 325, 880, 1050, 1087, 1456
BstFNI	CGCG	1 cut(s)	118
BstHHI	GCGC	1 cut(s)	239
BstKTI	GATC	8 cut(s)	134, 387, 667, 845, 1195, 1255, 1398, 1705
BstMBI	GATC	8 cut(s)	131, 384, 664, 842, 1192, 1252, 1395, 1702
BstMWI	GCNNNNNNNGC	7 cut(s)	35, 292, 794, 838, 847, 914, 1552
BstNI	CCWGG	4 cut(s)	215, 433, 858, 1692
BstNSI	RCATGY	1 cut(s)	464
BstSCI	CCNGG	5 cut(s)	213, 431, 856, 1646, 1690
BstSFI	CTRYAG	3 cut(s)	39, 495, 1141
BstSLI	GKGCMC	1 cut(s)	31
BstUI	CGCG	1 cut(s)	118
BstV1I	GCAGC	5 cut(s)	25, 298, 694, 775, 1429
BstV2I	GAAGAC	2 cut(s)	182, 1155
BstX2I	RGATCY	4 cut(s)	131, 664, 1252, 1702
BstXI	CCANNNNNNTGG	2 cut(s)	554, 1563
BstYI	RGATCY	4 cut(s)	131, 664, 1252, 1702
BsuI	GTATCC	1 cut(s)	872
BsuRI	GGCC	2 cut(s)	936, 991
BtsCI	GGATG	8 cut(s)	13, 99, 205, 325, 880, 1050, 1087, 1456
BtsIMutI	CAGTG	3 cut(s)	598, 1130, 1443
BtuMI	TCGCGA	1 cut(s)	118
Cac8I	GCNNGC	3 cut(s)	36, 241, 1548
CaiI	CAGNNNCTG	2 cut(s)	597, 1442
CfoI	GCGC	1 cut(s)	239
Cfr10I	RCCGGY	1 cut(s)	299
Cfr13I	GGNCC	3 cut(s)	395, 989, 1633
CseI	GACGC	1 cut(s)	964
CsiI	ACCWGGT	1 cut(s)	856
Csp6I	GTAC	3 cut(s)	303, 1460, 1567
CspAI	ACCGGT	1 cut(s)	299
CspCI	CAANNNNNGTGG	2 cut(s)	1585, 1620
CviAII	CATG	7 cut(s)	234, 389, 461, 554, 1153, 1298, 1411
CviQI	GTAC	3 cut(s)	303, 1460, 1567
DdeI	CTNAG	8 cut(s)	747, 798, 846, 968, 1005, 1212, 1326, 1551
DpnI	GATC	8 cut(s)	133, 386, 666, 844, 1194, 1254, 1397, 1704
DpnII	GATC	8 cut(s)	131, 384, 664, 842, 1192, 1252, 1395, 1702
DraI	TTTAAA	2 cut(s)	1225, 1431
EaeI	YGGCCR	1 cut(s)	934
Eam1104I	CTCTTC	2 cut(s)	1494, 1690
EarI	CTCTTC	2 cut(s)	1494, 1690
EciI	GGCGGA	1 cut(s)	20
Eco130I	CCWWGG	1 cut(s)	280
Eco32I	GATATC	1 cut(s)	1688
Eco47I	GGWCC	2 cut(s)	395, 1633
Eco57I	CTGAAG	1 cut(s)	233
Eco88I	CYCGRG	1 cut(s)	443
EcoNI	CCTNNNNNAGG	1 cut(s)	126
EcoRII	CCWGG	4 cut(s)	213, 431, 856, 1690
EcoRV	GATATC	1 cut(s)	1688
EcoT14I	CCWWGG	1 cut(s)	280
EcoT22I	ATGCAT	2 cut(s)	205, 520
ErhI	CCWWGG	1 cut(s)	280
FaeI	CATG	7 cut(s)	237, 392, 464, 557, 1156, 1301, 1414
FalI	AAGNNNNNCTT	2 cut(s)	1205, 1237
FaqI	GGGAC	1 cut(s)	1603
FatI	CATG	7 cut(s)	233, 388, 460, 553, 1152, 1297, 1410
FauI	CCCGC	1 cut(s)	1555
Fnu4HI	GCNGC	6 cut(s)	39, 287, 708, 789, 1443, 1580
FokI	GGATG	8 cut(s)	20, 86, 212, 332, 887, 1057, 1094, 1463
Fsp4HI	GCNGC	6 cut(s)	39, 287, 708, 789, 1443, 1580
FspBI	CTAG	1 cut(s)	1683
GlaI	GCGC	1 cut(s)	238
GluI	GCNGC	6 cut(s)	39, 287, 708, 789, 1443, 1580
GsaI	CCCAGC	3 cut(s)	34, 147, 410
GsuI	CTGGAG	2 cut(s)	236, 854
HaeIII	GGCC	2 cut(s)	936, 991
HapII	CCGG	3 cut(s)	300, 398, 1647
HgaI	GACGC	1 cut(s)	964
HhaI	GCGC	1 cut(s)	239
Hin1II	CATG	7 cut(s)	237, 392, 464, 557, 1156, 1301, 1414
Hin6I	GCGC	1 cut(s)	237
HinP1I	GCGC	1 cut(s)	237
HincII	GTYRAC	1 cut(s)	403
HindII	GTYRAC	1 cut(s)	403
HindIII	AAGCTT	3 cut(s)	332, 713, 848
HinfI	GANTC	5 cut(s)	11, 93, 507, 950, 1620
HpaII	CCGG	3 cut(s)	300, 398, 1647
HphI	GGTGA	6 cut(s)	184, 265, 394, 430, 577, 623
Hpy166II	GTNNAC	2 cut(s)	403, 1378
Hpy188I	TCNGA	7 cut(s)	88, 159, 512, 669, 691, 1327, 1664
Hpy188III	TCNNGA	3 cut(s)	117, 477, 1042
Hpy8I	GTNNAC	2 cut(s)	403, 1378
HpyAV	CCTTC	6 cut(s)	256, 257, 352, 373, 874, 1687
HpyCH4III	ACNGT	5 cut(s)	599, 928, 1094, 1438, 1594
HpyF10VI	GCNNNNNNNGC	7 cut(s)	35, 292, 794, 838, 847, 914, 1552
HpyF3I	CTNAG	8 cut(s)	747, 798, 846, 968, 1005, 1212, 1326, 1551
Hsp92II	CATG	7 cut(s)	237, 392, 464, 557, 1156, 1301, 1414
HspAI	GCGC	1 cut(s)	237
KpnI	GGTACC	2 cut(s)	1463, 1570
Kzo9I	GATC	8 cut(s)	131, 384, 664, 842, 1192, 1252, 1395, 1702
LguI	GCTCTTC	2 cut(s)	1494, 1690
LmnI	GCTCC	5 cut(s)	217, 364, 457, 838, 1051
Lsp1109I	GCAGC	5 cut(s)	25, 298, 694, 775, 1429
LweI	GCATC	4 cut(s)	49, 190, 1255, 1331
MabI	ACCWGGT	1 cut(s)	856
MaeI	CTAG	1 cut(s)	1683
MaeIII	GTNAC	6 cut(s)	43, 499, 593, 625, 1128, 1505
MalI	GATC	8 cut(s)	133, 386, 666, 844, 1194, 1254, 1397, 1704
MboI	GATC	8 cut(s)	131, 384, 664, 842, 1192, 1252, 1395, 1702
MboII	GAAGA	4 cut(s)	187, 1160, 1511, 1707
MflI	RGATCY	4 cut(s)	131, 664, 1252, 1702
MhlI	GDGCHC	3 cut(s)	31, 967, 1332
MlsI	TGGCCA	1 cut(s)	936
MluCI	AATT	9 cut(s)	153, 579, 780, 938, 1059, 1112, 1157, 1173, 1260
MluNI	TGGCCA	1 cut(s)	936
MlyI	GAGTC	1 cut(s)	102
MmeI	TCCRAC	1 cut(s)	636
Mox20I	TGGCCA	1 cut(s)	936
Mph1103I	ATGCAT	2 cut(s)	205, 520
MscI	TGGCCA	1 cut(s)	936
MseI	TTAA	7 cut(s)	50, 330, 804, 827, 852, 1224, 1430
MslI	CAYNNNNRTG	2 cut(s)	552, 1561
Msp20I	TGGCCA	1 cut(s)	936
MspA1I	CMGCKG	2 cut(s)	751, 1550
MspCI	CTTAAG	2 cut(s)	49, 329
MspI	CCGG	3 cut(s)	300, 398, 1647
MspR9I	CCNGG	5 cut(s)	215, 433, 858, 1648, 1692
Mva1269I	GAATGC	2 cut(s)	209, 1403
MvaI	CCWGG	4 cut(s)	215, 433, 858, 1692
MvnI	CGCG	1 cut(s)	118
MwoI	GCNNNNNNNGC	7 cut(s)	35, 292, 794, 838, 847, 914, 1552
NciI	CCSGG	1 cut(s)	1648
NdeII	GATC	8 cut(s)	131, 384, 664, 842, 1192, 1252, 1395, 1702
NlaIII	CATG	7 cut(s)	237, 392, 464, 557, 1156, 1301, 1414
NlaIV	GGNNCC	4 cut(s)	396, 1053, 1461, 1568
NmuCI	GTSAC	2 cut(s)	593, 625
NruI	TCGCGA	1 cut(s)	118
NsiI	ATGCAT	2 cut(s)	205, 520
NspI	RCATGY	1 cut(s)	464
NspV	TTCGAA	1 cut(s)	1623
PaeR7I	CTCGAG	1 cut(s)	443
PciSI	GCTCTTC	2 cut(s)	1494, 1690
PctI	GAATGC	2 cut(s)	209, 1403
PfeI	GAWTC	4 cut(s)	11, 507, 950, 1620
PfoI	TCCNGGA	2 cut(s)	213, 1690
PinAI	ACCGGT	1 cut(s)	299
PkrI	GCNGC	6 cut(s)	40, 288, 709, 790, 1444, 1581
PleI	GAGTC	1 cut(s)	101
PpsI	GAGTC	1 cut(s)	101
Psp6I	CCWGG	4 cut(s)	213, 431, 856, 1690
PspFI	CCCAGC	3 cut(s)	30, 143, 406
PspGI	CCWGG	4 cut(s)	213, 431, 856, 1690
PspN4I	GGNNCC	4 cut(s)	396, 1053, 1461, 1568
PspPI	GGNCC	3 cut(s)	395, 989, 1633
PspXI	VCTCGAGB	1 cut(s)	443
PstI	CTGCAG	3 cut(s)	43, 499, 1145
PstNI	CAGNNNCTG	2 cut(s)	597, 1442
PsuI	RGATCY	4 cut(s)	131, 664, 1252, 1702
PvuII	CAGCTG	1 cut(s)	751
RruI	TCGCGA	1 cut(s)	118
RsaI	GTAC	3 cut(s)	304, 1461, 1568
RsaNI	GTAC	3 cut(s)	303, 1460, 1567
RseI	CAYNNNNRTG	2 cut(s)	552, 1561
SapI	GCTCTTC	2 cut(s)	1494, 1690
SaqAI	TTAA	7 cut(s)	50, 330, 804, 827, 852, 1224, 1430
SatI	GCNGC	6 cut(s)	39, 287, 708, 789, 1443, 1580
Sau3AI	GATC	8 cut(s)	131, 384, 664, 842, 1192, 1252, 1395, 1702
Sau96I	GGNCC	3 cut(s)	395, 989, 1633
SchI	GAGTC	1 cut(s)	102
ScrFI	CCNGG	5 cut(s)	215, 433, 858, 1648, 1692
SduI	GDGCHC	3 cut(s)	31, 967, 1332
SexAI	ACCWGGT	1 cut(s)	856
SfaNI	GCATC	4 cut(s)	49, 190, 1255, 1331
SfcI	CTRYAG	3 cut(s)	39, 495, 1141
Sfr274I	CTCGAG	1 cut(s)	443
SfuI	TTCGAA	1 cut(s)	1623
SinI	GGWCC	2 cut(s)	395, 1633
SlaI	CTCGAG	1 cut(s)	443
SmiMI	CAYNNNNRTG	2 cut(s)	552, 1561
SmlI	CTYRAG	4 cut(s)	49, 329, 443, 1076
SmoI	CTYRAG	4 cut(s)	49, 329, 443, 1076
Sse9I	AATT	9 cut(s)	153, 579, 780, 938, 1059, 1112, 1157, 1173, 1260
SsiI	CCGC	3 cut(s)	5, 1548, 1579
SspMI	CTAG	1 cut(s)	1683
StyD4I	CCNGG	5 cut(s)	213, 431, 856, 1646, 1690
StyI	CCWWGG	1 cut(s)	280
TaaI	ACNGT	5 cut(s)	599, 928, 1094, 1438, 1594
TaqI	TCGA	5 cut(s)	444, 868, 948, 1000, 1623
TasI	AATT	9 cut(s)	153, 579, 780, 938, 1059, 1112, 1157, 1173, 1260
TauI	GCSGC	1 cut(s)	1582
TfiI	GAWTC	4 cut(s)	11, 507, 950, 1620
Tru1I	TTAA	7 cut(s)	50, 330, 804, 827, 852, 1224, 1430
Tru9I	TTAA	7 cut(s)	50, 330, 804, 827, 852, 1224, 1430
TscAI	CASTG	3 cut(s)	598, 1130, 1443
TseFI	GTSAC	2 cut(s)	593, 625
TseI	GCWGC	5 cut(s)	38, 286, 707, 788, 1442
Tsp45I	GTSAC	2 cut(s)	593, 625
TspDTI	ATGAA	2 cut(s)	455, 1103
TspGWI	ACGGA	3 cut(s)	55, 92, 1243
TspRI	CASTG	3 cut(s)	598, 1130, 1443
Vha464I	CTTAAG	2 cut(s)	49, 329
VpaK11BI	GGWCC	2 cut(s)	395, 1633
XagI	CCTNNNNNAGG	1 cut(s)	126
XapI	RAATTY	3 cut(s)	153, 1112, 1157
XceI	RCATGY	1 cut(s)	464
XhoI	CTCGAG	1 cut(s)	443
XspI	CTAG	1 cut(s)	1683
Zsp2I	ATGCAT	2 cut(s)	205, 520

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.