Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0005074

Physical Location & Seq

Genomic Coordinates Forward (+)

24011671 .. 24015843

Gene Structure 4173 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG204000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1599 bp

ATGGCTGCTGATTCGGCACAGTTAAGTTCCCAACTGGCTGAAGCAACTTTAAACGAATCTGTCTCTGCTCTGAAAGCAGAATTTTCTGACAGAGTTCCCATCAGATCCATAATATCACGAACCGATGGGGGGTCTGGGTTTGCCGGGCAGCATGTCCGGGTTGGTGGCTGGGTGAAGACGGGCAGGAAGGCAGACAAGGATGCATTTGCTTTTCTGGAACTTAATGATGGATCATGCCCTGGCAACTTACAGGTGATTGTGGAAGCAGACAAGGGTGACCTTGGGCAGCTTGTGCCAACCGGTACATGTGTGGTTGTGGATGGCGTGCTTAAGCTGCCCCCAGCTGGGGCCAAGCAGAAGGTGGAGCTTAGAGTTGAGAAGGTGGTCCATTTAGGTCCAGTTGACCCTGCTAAATATCCGTTGCCCAAGACCAAGCTCACCCTTGAGTTTTTGAGGGACGTTGTTCATCTCCGTTCCAGAACTAACACCATCTCTGCAGTTGCTCGCATCAGAAATGCCCTGGCATATGCAACTCATACATTTTTCCAAAAGCATGGCTTCCTTTATGTGCACACTCCAATTATCACTACCAGTGATTGTGAGGGTGCTGGTGAGATGTTCCAAGTCACAACATTGATTAGCGAAGGTGAAAGGTTGGAGAGGGAGCTGGTTAAGAACCCTCCCCCGTCTGAAGCAGACCTAGAAGCTGCCAAGCTAATCATCAAGGAAAAAGGAGATGCTGTTTCACAGCTGAAATCTGCTAAAGCAAGTAAGGAGGAGATTGGCGCTGCTGTCGCTGAACTTAAAAGGGCGAAGGAGAATGATTTGAAGCTGGAGGAGAGATCCAAGCTTCAGCCAGGAATCCCCAAAAAGGATGGGAAGATTGACTATACACAAGATTTCTTTGCCCGTCAAGCTTTTTTGACCGTTTCTGGCCAACTCCAAGTGGAATCTTATGCATGTGCTCTTAGTAGCGTGTATACATTTGGGCCTACTTTTCGTGCTGAGAATTCACACACTTCACGGCATTTGGCAGAATTCTGGATGGTGGAGCCTGAATTAGCATTTGCAGAGCTCAAGGATGACATGAACTGTGCAGAGGCGTATGTGAAATTCTTGTGTCAGTGGTTACTTGACAACTGCTCTGACGATATGGAGTTTATTTCTCGGCAATTTGATAAAACTTGCATAGACCGTCTAAAGATGGTTGCATCCACACCGTTTGAACGGATTACATATACAGAAGCTGTGGAGCTGCTAATTGATGCTGTGAAAAATGGCAAGAAGTTTGAGAATCATGTAGAGTGGGGGATTGACTTGGCATCTGAACATGAAAGATTCTTAACAGAGGTGAAATTTCAGAAGCCTGTTATTGTGTACAATTACCCAAAAGGGATCAAAGCTTTCTACATGAGACTCAATGATGATAACAAAACAGTGGCTGCTATGGATGTCCTTGTACCAAAGGTGGGCATTAACCTATCAAGGATTCTGTCAATGAGTTCTTTTTGCATGGTTCTTTTATACACTAATGTGTTGTGCATCTTTTGCTGTATTTACATTCATATTTTTTGCTTATATTTCAGGTGGGAGAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0007275 GO:0008150 GO:0009507 GO:0009536 GO:0009790 GO:0009791 GO:0009793 GO:0010035 GO:0010038 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

WHEP-TRS tRNA-synt_2 tRNA_anti-codon

Protein Analysis

532

Amino Acids

59.38

Weight (kDa)

6.19

Isoelectric Point (pI)

31.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA_anti-codon	PF01336	52 - 129	1.9e-08	OB-fold nucleic acid binding domain
tRNA-synt_2	PF00152	155 - 212	3.6e-07	tRNA synthetases class II (D, K and N)
WHEP-TRS	PF00458	241 - 277	1.9e-06	WHEP-TRS domain
tRNA-synt_2	PF00152	300 - 489	2.2e-25	tRNA synthetases class II (D, K and N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005074)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70980 AT5G56680
fragaria_vesca	FvH4_3g17430
malus_domestica	MD01G1115900.v1.1 MD03G1251800.v1.1 MD05G1158600.v1.1 MD11G1273000.v1.1
prunus_persica	Prupe.4G154500_v2.0.a1 Prupe.4G154500_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1
pyrus_communis	pycom03g19910 pycom05g15060 pycom11g24090
rosa_chinensis	RchiOBHm_Chr5g0028961
rosa_laevigata	RLG00000033131
rosa_multiflora	Rmu_co7984584.1_g000001 Rmu_sc0009042.1_g000005
rosa_roxburghii	Rroxscaffold_1G00050930
rosa_rugosa	Rorug05G0111900 Rorug05G0112000 Rorug05G0112000
rosa_samantha	Rh5AG204000 Rh5BG202300 Rh5CG223100 Rh5DG206100
rosa_wichuraiana	Rw5G018490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	980
AclWI	GGATC	4 cut(s)	99, 238, 837, 1403
AcoI	YGGCCR	1 cut(s)	934
AcsI	RAATTY	5 cut(s)	80, 1009, 1037, 1112, 1355
AcuI	CTGAAG	3 cut(s)	60, 711, 836
AfaI	GTAC	3 cut(s)	304, 1379, 1461
AfiI	CCNNNNNNNGG	6 cut(s)	129, 344, 345, 346, 871, 1469
AflII	CTTAAG	1 cut(s)	329
AflIII	ACRYGT	1 cut(s)	305
AgeI	ACCGGT	1 cut(s)	299
AgsI	TTSAA	2 cut(s)	829, 1226
AjnI	CCWGG	3 cut(s)	238, 519, 856
AleI	CACNNNNGTG	1 cut(s)	1532
Alw21I	GWGCWC	3 cut(s)	573, 967, 1077
Alw26I	GTCTC	2 cut(s)	67, 1408
Alw44I	GTGCAC	1 cut(s)	569
AlwI	GGATC	4 cut(s)	99, 238, 837, 1403
AlwNI	CAGNNNCTG	2 cut(s)	1247, 1442
AoxI	GGCC	3 cut(s)	348, 934, 989
ApaLI	GTGCAC	1 cut(s)	569
ApeKI	GCWGC	8 cut(s)	5, 148, 286, 334, 707, 788, 1255, 1442
ApoI	RAATTY	5 cut(s)	80, 1009, 1037, 1112, 1355
AsiGI	ACCGGT	1 cut(s)	299
AspLEI	GCGC	1 cut(s)	788
AspS9I	GGNCC	4 cut(s)	348, 385, 395, 989
AsuC2I	CCSGG	2 cut(s)	145, 158
AsuHPI	GGTGA	7 cut(s)	184, 265, 287, 430, 623, 659, 1363
AvaII	GGWCC	2 cut(s)	385, 395
BaeGI	GKGCMC	1 cut(s)	573
BaeI	ACNNNNGTAYC	2 cut(s)	294, 327
BalI	TGGCCA	1 cut(s)	936
BanII	GRGCYC	1 cut(s)	1077
BbsI	GAAGAC	1 cut(s)	182
Bbv12I	GWGCWC	3 cut(s)	573, 967, 1077
BbvI	GCAGC	7 cut(s)	160, 298, 321, 694, 775, 1242, 1429
BccI	CCATC	8 cut(s)	107, 119, 221, 314, 497, 869, 1039, 1198
BceAI	ACGGC	1 cut(s)	1040
BciT130I	CCWGG	3 cut(s)	240, 521, 858
BcnI	CCSGG	2 cut(s)	145, 158
BcoDI	GTCTC	2 cut(s)	67, 1408
BfaI	CTAG	1 cut(s)	701
BfmI	CTRYAG	1 cut(s)	495
BfoI	RGCGCY	1 cut(s)	789
BfrI	CTTAAG	1 cut(s)	329
BisI	GCNGC	8 cut(s)	6, 149, 287, 335, 708, 789, 1256, 1443
BlsI	GCNGC	8 cut(s)	7, 150, 288, 336, 709, 790, 1257, 1444
Bme1390I	CCNGG	5 cut(s)	145, 158, 240, 521, 858
Bme18I	GGWCC	2 cut(s)	385, 395
BmgT120I	GGNCC	4 cut(s)	348, 385, 395, 989
BmiI	GGNNCC	2 cut(s)	349, 1053
BmrFI	CCNGG	5 cut(s)	145, 158, 240, 521, 858
BmsI	GCATC	7 cut(s)	190, 516, 727, 1220, 1255, 1331, 1551
BpiI	GAAGAC	1 cut(s)	182
BpmI	CTGGAG	1 cut(s)	854
BpuEI	CTTGAG	2 cut(s)	464, 1061
BpuMI	CCSGG	2 cut(s)	145, 158
BsaJI	CCNNGG	3 cut(s)	238, 280, 519
BsaWI	WCCGGW	1 cut(s)	299
BsaXI	ACNNNNNCTCC	2 cut(s)	726, 756
Bsc4I	CCNNNNNNNGG	6 cut(s)	129, 344, 345, 346, 871, 1469
Bse118I	RCCGGY	1 cut(s)	299
Bse1I	ACTGG	3 cut(s)	39, 398, 591
BseBI	CCWGG	3 cut(s)	240, 521, 858
BseDI	CCNNGG	3 cut(s)	238, 280, 519
BseGI	GGATG	7 cut(s)	205, 325, 880, 1050, 1087, 1211, 1456
BseLI	CCNNNNNNNGG	6 cut(s)	129, 344, 345, 346, 871, 1469
BseMII	CTCAG	1 cut(s)	996
BseNI	ACTGG	3 cut(s)	39, 398, 591
BseRI	GAGGAG	2 cut(s)	791, 851
BseSI	GKGCMC	1 cut(s)	573
BseXI	GCAGC	7 cut(s)	160, 298, 321, 694, 775, 1242, 1429
BseYI	CCCAGC	3 cut(s)	168, 340, 344
BsgI	GTGCAG	1 cut(s)	1116
BshFI	GGCC	3 cut(s)	350, 936, 991
BshTI	ACCGGT	1 cut(s)	299
BsiHKAI	GWGCWC	3 cut(s)	573, 967, 1077
BsiSI	CCGG	3 cut(s)	144, 157, 300
BslFI	GGGAC	1 cut(s)	470
BslI	CCNNNNNNNGG	6 cut(s)	129, 344, 345, 346, 871, 1469
BsmAI	GTCTC	2 cut(s)	67, 1408
BsmFI	GGGAC	1 cut(s)	470
BsnI	GGCC	3 cut(s)	350, 936, 991
Bsp1286I	GDGCHC	3 cut(s)	573, 967, 1077
Bsp1407I	TGTACA	1 cut(s)	1377
Bsp143I	GATC	4 cut(s)	104, 230, 842, 1395
BspANI	GGCC	3 cut(s)	350, 936, 991
BspCNI	CTCAG	1 cut(s)	997
BspLI	GGNNCC	2 cut(s)	349, 1053
BspMAI	CTGCAG	1 cut(s)	499
BspPI	GGATC	4 cut(s)	99, 238, 837, 1403
BspTI	CTTAAG	1 cut(s)	329
BsrFI	RCCGGY	1 cut(s)	299
BsrGI	TGTACA	1 cut(s)	1377
BsrI	ACTGG	3 cut(s)	39, 398, 591
BssAI	RCCGGY	1 cut(s)	299
BssECI	CCNNGG	3 cut(s)	238, 280, 519
BssMI	GATC	4 cut(s)	104, 230, 842, 1395
BssNAI	GTATAC	1 cut(s)	981
BssT1I	CCWWGG	1 cut(s)	280
Bst1107I	GTATAC	1 cut(s)	981
Bst2UI	CCWGG	3 cut(s)	240, 521, 858
Bst4CI	ACNGT	6 cut(s)	21, 928, 1094, 1196, 1221, 1438
BstAFI	CTTAAG	1 cut(s)	329
BstAPI	GCANNNNNTGC	2 cut(s)	292, 1548
BstAUI	TGTACA	1 cut(s)	1377
BstC8I	GCNNGC	2 cut(s)	326, 505
BstDEI	CTNAG	3 cut(s)	368, 968, 1005
BstEII	GGTNACC	1 cut(s)	275
BstF5I	GGATG	7 cut(s)	205, 325, 880, 1050, 1087, 1211, 1456
BstH2I	RGCGCY	1 cut(s)	789
BstHHI	GCGC	1 cut(s)	788
BstKTI	GATC	4 cut(s)	107, 233, 845, 1398
BstMAI	GTCTC	2 cut(s)	67, 1408
BstMBI	GATC	4 cut(s)	104, 230, 842, 1395
BstMWI	GCNNNNNNNGC	7 cut(s)	14, 74, 292, 334, 794, 914, 1548
BstNI	CCWGG	3 cut(s)	240, 521, 858
BstNSI	RCATGY	3 cut(s)	155, 309, 963
BstPI	GGTNACC	1 cut(s)	275
BstSCI	CCNGG	5 cut(s)	143, 156, 238, 519, 856
BstSFI	CTRYAG	1 cut(s)	495
BstSLI	GKGCMC	1 cut(s)	573
BstV1I	GCAGC	7 cut(s)	160, 298, 321, 694, 775, 1242, 1429
BstV2I	GAAGAC	1 cut(s)	182
BstX2I	RGATCY	2 cut(s)	104, 842
BstXI	CCANNNNNNTGG	1 cut(s)	554
BstYI	RGATCY	2 cut(s)	104, 842
BstZ17I	GTATAC	1 cut(s)	981
BsuRI	GGCC	3 cut(s)	350, 936, 991
BtsCI	GGATG	7 cut(s)	205, 325, 880, 1050, 1087, 1211, 1456
BtsIMutI	CAGTG	3 cut(s)	598, 1130, 1443
Cac8I	GCNNGC	2 cut(s)	326, 505
CaiI	CAGNNNCTG	2 cut(s)	1247, 1442
CfoI	GCGC	1 cut(s)	788
Cfr10I	RCCGGY	1 cut(s)	299
Cfr13I	GGNCC	4 cut(s)	348, 385, 395, 989
Csp6I	GTAC	3 cut(s)	303, 1378, 1460
CspAI	ACCGGT	1 cut(s)	299
CspCI	CAANNNNNGTGG	2 cut(s)	1204, 1239
CviQI	GTAC	3 cut(s)	303, 1378, 1460
DdeI	CTNAG	3 cut(s)	368, 968, 1005
DpnI	GATC	4 cut(s)	106, 232, 844, 1397
DpnII	GATC	4 cut(s)	104, 230, 842, 1395
DraI	TTTAAA	1 cut(s)	51
EaeI	YGGCCR	1 cut(s)	934
Ecl136II	GAGCTC	1 cut(s)	1075
Eco130I	CCWWGG	1 cut(s)	280
Eco24I	GRGCYC	1 cut(s)	1077
Eco47I	GGWCC	2 cut(s)	385, 395
Eco53kI	GAGCTC	1 cut(s)	1075
Eco57I	CTGAAG	3 cut(s)	60, 711, 836
Eco91I	GGTNACC	1 cut(s)	275
EcoICRI	GAGCTC	1 cut(s)	1075
EcoO65I	GGTNACC	1 cut(s)	275
EcoRI	GAATTC	2 cut(s)	1009, 1037
EcoRII	CCWGG	3 cut(s)	238, 519, 856
EcoT14I	CCWWGG	1 cut(s)	280
EcoT22I	ATGCAT	2 cut(s)	205, 961
EcoT38I	GRGCYC	1 cut(s)	1077
ErhI	CCWWGG	1 cut(s)	280
FalI	AAGNNNNNCTT	2 cut(s)	542, 574
FaqI	GGGAC	1 cut(s)	470
FauNDI	CATATG	1 cut(s)	526
FblI	GTMKAC	1 cut(s)	980
Fnu4HI	GCNGC	8 cut(s)	6, 149, 287, 335, 708, 789, 1256, 1443
FokI	GGATG	7 cut(s)	212, 332, 887, 1057, 1094, 1198, 1463
FriOI	GRGCYC	1 cut(s)	1077
Fsp4HI	GCNGC	8 cut(s)	6, 149, 287, 335, 708, 789, 1256, 1443
FspBI	CTAG	1 cut(s)	701
GlaI	GCGC	1 cut(s)	787
GluI	GCNGC	8 cut(s)	6, 149, 287, 335, 708, 789, 1256, 1443
GsaI	CCCAGC	3 cut(s)	172, 344, 348
GsuI	CTGGAG	1 cut(s)	854
HaeII	RGCGCY	1 cut(s)	789
HaeIII	GGCC	3 cut(s)	350, 936, 991
HapII	CCGG	3 cut(s)	144, 157, 300
HhaI	GCGC	1 cut(s)	788
Hin6I	GCGC	1 cut(s)	786
HinP1I	GCGC	1 cut(s)	786
HincII	GTYRAC	1 cut(s)	403
HindII	GTYRAC	1 cut(s)	403
HindIII	AAGCTT	3 cut(s)	848, 915, 1401
HinfI	GANTC	8 cut(s)	11, 56, 861, 950, 1294, 1338, 1416, 1489
HpaII	CCGG	3 cut(s)	144, 157, 300
HphI	GGTGA	7 cut(s)	184, 265, 287, 430, 623, 659, 1363
Hpy166II	GTNNAC	4 cut(s)	403, 571, 981, 1378
Hpy188I	TCNGA	8 cut(s)	72, 88, 104, 512, 691, 1147, 1327, 1362
Hpy188III	TCNNGA	4 cut(s)	117, 215, 477, 1042
Hpy8I	GTNNAC	4 cut(s)	403, 571, 981, 1378
HpyAV	CCTTC	5 cut(s)	181, 352, 373, 638, 808
HpyCH4III	ACNGT	6 cut(s)	21, 928, 1094, 1196, 1221, 1438
HpyCH4IV	ACGT	1 cut(s)	459
HpyF10VI	GCNNNNNNNGC	7 cut(s)	14, 74, 292, 334, 794, 914, 1548
HpyF3I	CTNAG	3 cut(s)	368, 968, 1005
HpySE526I	ACGT	1 cut(s)	459
HspAI	GCGC	1 cut(s)	786
Kzo9I	GATC	4 cut(s)	104, 230, 842, 1395
LmnI	GCTCC	4 cut(s)	364, 664, 1051, 1252
Lsp1109I	GCAGC	7 cut(s)	160, 298, 321, 694, 775, 1242, 1429
LweI	GCATC	7 cut(s)	190, 516, 727, 1220, 1255, 1331, 1551
MaeI	CTAG	1 cut(s)	701
MaeII	ACGT	1 cut(s)	459
MaeIII	GTNAC	3 cut(s)	275, 625, 1128
MalI	GATC	4 cut(s)	106, 232, 844, 1397
MboI	GATC	4 cut(s)	104, 230, 842, 1395
MboII	GAAGA	2 cut(s)	187, 892
MflI	RGATCY	2 cut(s)	104, 842
MhlI	GDGCHC	3 cut(s)	573, 967, 1077
MlsI	TGGCCA	1 cut(s)	936
MluNI	TGGCCA	1 cut(s)	936
MlyI	GAGTC	1 cut(s)	1410
MmeI	TCCRAC	1 cut(s)	636
MnlI	CCTC	8 cut(s)	447, 595, 654, 690, 769, 829, 1093, 1342
Mox20I	TGGCCA	1 cut(s)	936
Mph1103I	ATGCAT	2 cut(s)	205, 961
MscI	TGGCCA	1 cut(s)	936
MseI	TTAA	8 cut(s)	23, 50, 222, 330, 672, 804, 1343, 1476
MslI	CAYNNNNRTG	1 cut(s)	1532
Msp20I	TGGCCA	1 cut(s)	936
MspA1I	CMGCKG	2 cut(s)	344, 751
MspCI	CTTAAG	1 cut(s)	329
MspI	CCGG	3 cut(s)	144, 157, 300
MspR9I	CCNGG	5 cut(s)	145, 158, 240, 521, 858
MvaI	CCWGG	3 cut(s)	240, 521, 858
MwoI	GCNNNNNNNGC	7 cut(s)	14, 74, 292, 334, 794, 914, 1548
NciI	CCSGG	2 cut(s)	145, 158
NdeI	CATATG	1 cut(s)	526
NdeII	GATC	4 cut(s)	104, 230, 842, 1395
NlaIV	GGNNCC	2 cut(s)	349, 1053
NmeAIII	GCCGAG	1 cut(s)	1147
NmuCI	GTSAC	2 cut(s)	275, 625
NsiI	ATGCAT	2 cut(s)	205, 961
NspI	RCATGY	3 cut(s)	155, 309, 963
OliI	CACNNNNGTG	1 cut(s)	1532
PciI	ACATGT	1 cut(s)	305
PfeI	GAWTC	7 cut(s)	11, 56, 861, 950, 1294, 1338, 1489
PinAI	ACCGGT	1 cut(s)	299
PkrI	GCNGC	8 cut(s)	7, 150, 288, 336, 709, 790, 1257, 1444
PleI	GAGTC	1 cut(s)	1410
PpsI	GAGTC	1 cut(s)	1410
PscI	ACATGT	1 cut(s)	305
Psp124BI	GAGCTC	1 cut(s)	1077
Psp6I	CCWGG	3 cut(s)	238, 519, 856
PspEI	GGTNACC	1 cut(s)	275
PspFI	CCCAGC	3 cut(s)	168, 340, 344
PspGI	CCWGG	3 cut(s)	238, 519, 856
PspN4I	GGNNCC	2 cut(s)	349, 1053
PspPI	GGNCC	4 cut(s)	348, 385, 395, 989
PstI	CTGCAG	1 cut(s)	499
PstNI	CAGNNNCTG	2 cut(s)	1247, 1442
PsuI	RGATCY	2 cut(s)	104, 842
PvuII	CAGCTG	2 cut(s)	344, 751
RsaI	GTAC	3 cut(s)	304, 1379, 1461
RsaNI	GTAC	3 cut(s)	303, 1378, 1460
RseI	CAYNNNNRTG	1 cut(s)	1532
SacI	GAGCTC	1 cut(s)	1077
SaqAI	TTAA	8 cut(s)	23, 50, 222, 330, 672, 804, 1343, 1476
SatI	GCNGC	8 cut(s)	6, 149, 287, 335, 708, 789, 1256, 1443
Sau3AI	GATC	4 cut(s)	104, 230, 842, 1395
Sau96I	GGNCC	4 cut(s)	348, 385, 395, 989
SchI	GAGTC	1 cut(s)	1410
ScrFI	CCNGG	5 cut(s)	145, 158, 240, 521, 858
SduI	GDGCHC	3 cut(s)	573, 967, 1077
SfaNI	GCATC	7 cut(s)	190, 516, 727, 1220, 1255, 1331, 1551
SfcI	CTRYAG	1 cut(s)	495
SinI	GGWCC	2 cut(s)	385, 395
SmiMI	CAYNNNNRTG	1 cut(s)	1532
SmlI	CTYRAG	3 cut(s)	329, 443, 1076
SmoI	CTYRAG	3 cut(s)	329, 443, 1076
SspMI	CTAG	1 cut(s)	701
SstI	GAGCTC	1 cut(s)	1077
StyD4I	CCNGG	5 cut(s)	143, 156, 238, 519, 856
StyI	CCWWGG	1 cut(s)	280
TaaI	ACNGT	6 cut(s)	21, 928, 1094, 1196, 1221, 1438
TaiI	ACGT	1 cut(s)	462
TatI	WGTACW	1 cut(s)	1377
TfiI	GAWTC	7 cut(s)	11, 56, 861, 950, 1294, 1338, 1489
Tru1I	TTAA	8 cut(s)	23, 50, 222, 330, 672, 804, 1343, 1476
Tru9I	TTAA	8 cut(s)	23, 50, 222, 330, 672, 804, 1343, 1476
TscAI	CASTG	3 cut(s)	598, 1130, 1443
TseFI	GTSAC	2 cut(s)	275, 625
TseI	GCWGC	8 cut(s)	5, 148, 286, 334, 707, 788, 1255, 1442
Tsp45I	GTSAC	2 cut(s)	275, 625
TspDTI	ATGAA	4 cut(s)	455, 1103, 1347, 1553
TspGWI	ACGGA	3 cut(s)	408, 461, 1243
TspRI	CASTG	3 cut(s)	598, 1130, 1443
Vha464I	CTTAAG	1 cut(s)	329
VneI	GTGCAC	1 cut(s)	569
VpaK11BI	GGWCC	2 cut(s)	385, 395
XapI	RAATTY	5 cut(s)	80, 1009, 1037, 1112, 1355
XceI	RCATGY	3 cut(s)	155, 309, 963
XcmI	CCANNNNNNNNNTGG	1 cut(s)	358
XmiI	GTMKAC	1 cut(s)	980
XspI	CTAG	1 cut(s)	701
Zsp2I	ATGCAT	2 cut(s)	205, 961

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.