Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0005074

Physical Location & Seq

Genomic Coordinates Forward (+)

22739898 .. 22744012

Gene Structure 4115 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG223100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1386 bp

ATGGCTGCTGATTCGGCACAGTTAAGTTCCCAACTGGCTGAAGCAACTTTAAACGGATCTGTCTCTGCTCTGAAAGCAGAATTTTCTGACAGGGTTCCCATCAGATCCATAATATCACGAACCGATGGGGGGTCTGGGTTTTCCGGGCAGCATGTCCGGGTTGGTGGTTGGGTGAAGACGGGCAGGAAGGCAGACAAGGATGCATTTGCTTTTCTGGAACTTAATGATGGATCATGCCCTGGCAACTTACAGGTGATTGTGGAAGCAGACAAGGGTGACCTTGGGCAGCTTGTGCCAACCGGAACATGTGTGGTTGTGGATGGCGTGCTTAAGCTGCCCCCAGCTGGGGCCAAGCAGAAGGTGGAGCTTAGAGTTGAGAAGGTGGTCCATTTAGGTCCAGTTGACCCTGCTAAATATCCGTTGCCCAAGACCAAGCTCACCCTTGAGTTTTTGAGGGACGTTGTTCATCTCCGTTCCAGAACTAACACCATCTCTGCAGTTGCTCGCATCAGAAATGCCCTGGCATATGCAACTCATACATTTTTCCAAAAGCATGGCTTCCTTTATGTGCACACTCCAATTATCACTACCAGTGATTGTGAGGGTGCTGGTGAGATGTTCCAAGTCACAACATTGATTAGCGAAGGTGAAAGGTTGGAGAGGGAGCTGATTAAGAACCCTCCCCCATCTGAAGCAGACCTAGAAGCTGCCAAGCTAATCATCAAGGAAAAAGGAGATGCTGTTTCACAGCTGAAATCTGCTAAAGCAAGTAAGGAGGAGATTGGCGCTGCTGTCGCTGAACTTAAAAGGGCGAAGGAGAATGATTTGAAGCTGGAGGAGAGATCCAAGCTTCAGCCAGGAATCCCCAAAAAGGATGGGAAGATTGACTATACACAAGATTTCTTTGCCCGTCAAGCTTTTTTGACCGTTTCTGGCCAACTCCAAGTGGAATCTTATGCATGTGCTCTTAGTAGCGTGTATACATTTGGGCCTACTTTTCGTGCTGAGAATTCACACACTTCACGGCATTTGGCAGAATTCTGGATGGTGGAGCCTGAATTAGCATTTGCAGAGCTCAAGGATGACATGAACTGTGCAGAGGCGTATGTGAAATTCTTGTGTCAGTGGTTACTTGACAACTGCTCTGACGATATGGAGTTTATTTCTCGGCAATTTGATAAAACTTGCATAGACCGTCTAAAGATGGTTGCATCCACACCGTTTGAACGGATTACATATACAGAAGCTGTGGAGCTGCTAATTGATGCTGTGAAAAATGGCAAGAAGTTTGAGAATCATGTAGAGTGGGGGATTGACTTGGCATCTGAACATGAAAGGTACTCCTGCATCATTGTTCTGCTCGATCAGTCGTATTATGGCTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0007275 GO:0008150 GO:0009507 GO:0009536 GO:0009790 GO:0009791 GO:0009793 GO:0010035 GO:0010038 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

WHEP-TRS tRNA-synt_2 tRNA_anti-codon

Protein Analysis

461

Amino Acids

50.98

Weight (kDa)

5.71

Isoelectric Point (pI)

27.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA_anti-codon	PF01336	52 - 129	1.6e-08	OB-fold nucleic acid binding domain
tRNA-synt_2	PF00152	155 - 212	2.9e-07	tRNA synthetases class II (D, K and N)
WHEP-TRS	PF00458	241 - 277	1.6e-06	WHEP-TRS domain
tRNA-synt_2	PF00152	300 - 433	5.4e-18	tRNA synthetases class II (D, K and N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005074)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70980 AT5G56680
fragaria_vesca	FvH4_3g17430
malus_domestica	MD01G1115900.v1.1 MD03G1251800.v1.1 MD05G1158600.v1.1 MD11G1273000.v1.1
prunus_persica	Prupe.4G154500_v2.0.a1 Prupe.4G154500_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1 Prupe.7G068600_v2.0.a1
pyrus_communis	pycom03g19910 pycom05g15060 pycom11g24090
rosa_chinensis	RchiOBHm_Chr5g0028961
rosa_laevigata	RLG00000033131
rosa_multiflora	Rmu_co7984584.1_g000001 Rmu_sc0009042.1_g000005
rosa_roxburghii	Rroxscaffold_1G00050930
rosa_rugosa	Rorug05G0111900 Rorug05G0112000 Rorug05G0112000
rosa_samantha	Rh5AG204000 Rh5BG202300 Rh5CG223100 Rh5DG206100
rosa_wichuraiana	Rw5G018490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	980
AclWI	GGATC	4 cut(s)	64, 99, 238, 837
AcoI	YGGCCR	1 cut(s)	934
AcsI	RAATTY	4 cut(s)	80, 1009, 1037, 1112
AcuI	CTGAAG	3 cut(s)	60, 711, 836
AfaI	GTAC	1 cut(s)	1340
AfiI	CCNNNNNNNGG	5 cut(s)	129, 344, 345, 346, 871
AflII	CTTAAG	1 cut(s)	329
AflIII	ACRYGT	1 cut(s)	305
AgsI	TTSAA	2 cut(s)	829, 1226
AjnI	CCWGG	3 cut(s)	238, 519, 856
Alw21I	GWGCWC	3 cut(s)	573, 967, 1077
Alw26I	GTCTC	1 cut(s)	67
Alw44I	GTGCAC	1 cut(s)	569
AlwI	GGATC	4 cut(s)	64, 99, 238, 837
AlwNI	CAGNNNCTG	1 cut(s)	1247
AoxI	GGCC	3 cut(s)	348, 934, 989
ApaLI	GTGCAC	1 cut(s)	569
ApeKI	GCWGC	8 cut(s)	5, 148, 286, 334, 707, 788, 1255, 1380
ApoI	RAATTY	4 cut(s)	80, 1009, 1037, 1112
AspLEI	GCGC	1 cut(s)	788
AspS9I	GGNCC	4 cut(s)	348, 385, 395, 989
AsuC2I	CCSGG	2 cut(s)	145, 158
AsuHPI	GGTGA	6 cut(s)	184, 265, 287, 430, 623, 659
AvaII	GGWCC	2 cut(s)	385, 395
BaeGI	GKGCMC	1 cut(s)	573
BalI	TGGCCA	1 cut(s)	936
BanII	GRGCYC	1 cut(s)	1077
BbsI	GAAGAC	1 cut(s)	182
Bbv12I	GWGCWC	3 cut(s)	573, 967, 1077
BbvI	GCAGC	7 cut(s)	160, 298, 321, 694, 775, 1242, 1367
BccI	CCATC	9 cut(s)	107, 119, 221, 314, 497, 694, 869, 1039, 1198
BceAI	ACGGC	1 cut(s)	1040
BciT130I	CCWGG	3 cut(s)	240, 521, 858
BcnI	CCSGG	2 cut(s)	145, 158
BcoDI	GTCTC	1 cut(s)	67
BfaI	CTAG	1 cut(s)	701
BfmI	CTRYAG	1 cut(s)	495
BfoI	RGCGCY	1 cut(s)	789
BfrI	CTTAAG	1 cut(s)	329
BisI	GCNGC	8 cut(s)	6, 149, 287, 335, 708, 789, 1256, 1381
BlsI	GCNGC	8 cut(s)	7, 150, 288, 336, 709, 790, 1257, 1382
Bme1390I	CCNGG	5 cut(s)	145, 158, 240, 521, 858
Bme18I	GGWCC	2 cut(s)	385, 395
BmgT120I	GGNCC	4 cut(s)	348, 385, 395, 989
BmiI	GGNNCC	3 cut(s)	96, 349, 1053
BmrFI	CCNGG	5 cut(s)	145, 158, 240, 521, 858
BmsI	GCATC	7 cut(s)	190, 516, 727, 1220, 1255, 1331, 1356
BpiI	GAAGAC	1 cut(s)	182
BpmI	CTGGAG	1 cut(s)	854
BpuEI	CTTGAG	2 cut(s)	464, 1061
BpuMI	CCSGG	2 cut(s)	145, 158
BsaJI	CCNNGG	3 cut(s)	238, 280, 519
BsaWI	WCCGGW	1 cut(s)	299
BsaXI	ACNNNNNCTCC	2 cut(s)	726, 756
Bsc4I	CCNNNNNNNGG	5 cut(s)	129, 344, 345, 346, 871
Bse1I	ACTGG	3 cut(s)	39, 398, 591
BseBI	CCWGG	3 cut(s)	240, 521, 858
BseDI	CCNNGG	3 cut(s)	238, 280, 519
BseGI	GGATG	6 cut(s)	205, 325, 880, 1050, 1087, 1211
BseLI	CCNNNNNNNGG	5 cut(s)	129, 344, 345, 346, 871
BseMII	CTCAG	1 cut(s)	996
BseNI	ACTGG	3 cut(s)	39, 398, 591
BseRI	GAGGAG	2 cut(s)	791, 851
BseSI	GKGCMC	1 cut(s)	573
BseXI	GCAGC	7 cut(s)	160, 298, 321, 694, 775, 1242, 1367
BseYI	CCCAGC	2 cut(s)	340, 344
BsgI	GTGCAG	1 cut(s)	1116
BshFI	GGCC	3 cut(s)	350, 936, 991
BsiHKAI	GWGCWC	3 cut(s)	573, 967, 1077
BsiSI	CCGG	3 cut(s)	144, 157, 300
BslFI	GGGAC	1 cut(s)	470
BslI	CCNNNNNNNGG	5 cut(s)	129, 344, 345, 346, 871
BsmAI	GTCTC	1 cut(s)	67
BsmFI	GGGAC	1 cut(s)	470
BsnI	GGCC	3 cut(s)	350, 936, 991
Bsp1286I	GDGCHC	3 cut(s)	573, 967, 1077
Bsp143I	GATC	5 cut(s)	56, 104, 230, 842, 1363
BspANI	GGCC	3 cut(s)	350, 936, 991
BspCNI	CTCAG	1 cut(s)	997
BspLI	GGNNCC	3 cut(s)	96, 349, 1053
BspMAI	CTGCAG	1 cut(s)	499
BspPI	GGATC	4 cut(s)	64, 99, 238, 837
BspTI	CTTAAG	1 cut(s)	329
BsrI	ACTGG	3 cut(s)	39, 398, 591
BssECI	CCNNGG	3 cut(s)	238, 280, 519
BssMI	GATC	5 cut(s)	56, 104, 230, 842, 1363
BssNAI	GTATAC	1 cut(s)	981
BssT1I	CCWWGG	1 cut(s)	280
Bst1107I	GTATAC	1 cut(s)	981
Bst2UI	CCWGG	3 cut(s)	240, 521, 858
Bst4CI	ACNGT	5 cut(s)	21, 928, 1094, 1196, 1221
BstAFI	CTTAAG	1 cut(s)	329
BstAPI	GCANNNNNTGC	1 cut(s)	292
BstC8I	GCNNGC	2 cut(s)	326, 505
BstDEI	CTNAG	3 cut(s)	368, 968, 1005
BstEII	GGTNACC	1 cut(s)	275
BstF5I	GGATG	6 cut(s)	205, 325, 880, 1050, 1087, 1211
BstH2I	RGCGCY	1 cut(s)	789
BstHHI	GCGC	1 cut(s)	788
BstKTI	GATC	5 cut(s)	59, 107, 233, 845, 1366
BstMAI	GTCTC	1 cut(s)	67
BstMBI	GATC	5 cut(s)	56, 104, 230, 842, 1363
BstMWI	GCNNNNNNNGC	6 cut(s)	14, 74, 292, 334, 794, 914
BstNI	CCWGG	3 cut(s)	240, 521, 858
BstNSI	RCATGY	3 cut(s)	155, 309, 963
BstPI	GGTNACC	1 cut(s)	275
BstSCI	CCNGG	5 cut(s)	143, 156, 238, 519, 856
BstSFI	CTRYAG	1 cut(s)	495
BstSLI	GKGCMC	1 cut(s)	573
BstV1I	GCAGC	7 cut(s)	160, 298, 321, 694, 775, 1242, 1367
BstV2I	GAAGAC	1 cut(s)	182
BstX2I	RGATCY	3 cut(s)	56, 104, 842
BstXI	CCANNNNNNTGG	1 cut(s)	554
BstYI	RGATCY	3 cut(s)	56, 104, 842
BstZ17I	GTATAC	1 cut(s)	981
BsuRI	GGCC	3 cut(s)	350, 936, 991
BtsCI	GGATG	6 cut(s)	205, 325, 880, 1050, 1087, 1211
BtsIMutI	CAGTG	2 cut(s)	598, 1130
Cac8I	GCNNGC	2 cut(s)	326, 505
CaiI	CAGNNNCTG	1 cut(s)	1247
CfoI	GCGC	1 cut(s)	788
Cfr13I	GGNCC	4 cut(s)	348, 385, 395, 989
Csp6I	GTAC	1 cut(s)	1339
CspCI	CAANNNNNGTGG	2 cut(s)	1204, 1239
CviAII	CATG	8 cut(s)	152, 234, 306, 554, 960, 1087, 1298, 1331
CviQI	GTAC	1 cut(s)	1339
DdeI	CTNAG	3 cut(s)	368, 968, 1005
DpnI	GATC	5 cut(s)	58, 106, 232, 844, 1365
DpnII	GATC	5 cut(s)	56, 104, 230, 842, 1363
DraI	TTTAAA	1 cut(s)	51
EaeI	YGGCCR	1 cut(s)	934
Ecl136II	GAGCTC	1 cut(s)	1075
Eco130I	CCWWGG	1 cut(s)	280
Eco24I	GRGCYC	1 cut(s)	1077
Eco47I	GGWCC	2 cut(s)	385, 395
Eco53kI	GAGCTC	1 cut(s)	1075
Eco57I	CTGAAG	3 cut(s)	60, 711, 836
Eco91I	GGTNACC	1 cut(s)	275
EcoICRI	GAGCTC	1 cut(s)	1075
EcoO65I	GGTNACC	1 cut(s)	275
EcoRI	GAATTC	2 cut(s)	1009, 1037
EcoRII	CCWGG	3 cut(s)	238, 519, 856
EcoT14I	CCWWGG	1 cut(s)	280
EcoT22I	ATGCAT	2 cut(s)	205, 961
EcoT38I	GRGCYC	1 cut(s)	1077
ErhI	CCWWGG	1 cut(s)	280
FaeI	CATG	8 cut(s)	155, 237, 309, 557, 963, 1090, 1301, 1334
FalI	AAGNNNNNCTT	2 cut(s)	542, 574
FaqI	GGGAC	1 cut(s)	470
FatI	CATG	8 cut(s)	151, 233, 305, 553, 959, 1086, 1297, 1330
FauNDI	CATATG	1 cut(s)	526
FblI	GTMKAC	1 cut(s)	980
Fnu4HI	GCNGC	8 cut(s)	6, 149, 287, 335, 708, 789, 1256, 1381
FokI	GGATG	6 cut(s)	212, 332, 887, 1057, 1094, 1198
FriOI	GRGCYC	1 cut(s)	1077
Fsp4HI	GCNGC	8 cut(s)	6, 149, 287, 335, 708, 789, 1256, 1381
FspBI	CTAG	1 cut(s)	701
GlaI	GCGC	1 cut(s)	787
GluI	GCNGC	8 cut(s)	6, 149, 287, 335, 708, 789, 1256, 1381
GsaI	CCCAGC	2 cut(s)	344, 348
GsuI	CTGGAG	1 cut(s)	854
HaeII	RGCGCY	1 cut(s)	789
HaeIII	GGCC	3 cut(s)	350, 936, 991
HapII	CCGG	3 cut(s)	144, 157, 300
HhaI	GCGC	1 cut(s)	788
Hin1II	CATG	8 cut(s)	155, 237, 309, 557, 963, 1090, 1301, 1334
Hin6I	GCGC	1 cut(s)	786
HinP1I	GCGC	1 cut(s)	786
HincII	GTYRAC	1 cut(s)	403
HindII	GTYRAC	1 cut(s)	403
HindIII	AAGCTT	2 cut(s)	848, 915
HinfI	GANTC	4 cut(s)	11, 861, 950, 1294
HpaII	CCGG	3 cut(s)	144, 157, 300
HphI	GGTGA	6 cut(s)	184, 265, 287, 430, 623, 659
Hpy166II	GTNNAC	3 cut(s)	403, 571, 981
Hpy188I	TCNGA	7 cut(s)	72, 88, 104, 512, 691, 1147, 1327
Hpy188III	TCNNGA	4 cut(s)	117, 215, 477, 1042
Hpy8I	GTNNAC	3 cut(s)	403, 571, 981
HpyAV	CCTTC	5 cut(s)	181, 352, 373, 638, 808
HpyCH4III	ACNGT	5 cut(s)	21, 928, 1094, 1196, 1221
HpyCH4IV	ACGT	1 cut(s)	459
HpyF10VI	GCNNNNNNNGC	6 cut(s)	14, 74, 292, 334, 794, 914
HpyF3I	CTNAG	3 cut(s)	368, 968, 1005
HpySE526I	ACGT	1 cut(s)	459
Hsp92II	CATG	8 cut(s)	155, 237, 309, 557, 963, 1090, 1301, 1334
HspAI	GCGC	1 cut(s)	786
Kzo9I	GATC	5 cut(s)	56, 104, 230, 842, 1363
LmnI	GCTCC	4 cut(s)	364, 664, 1051, 1252
Lsp1109I	GCAGC	7 cut(s)	160, 298, 321, 694, 775, 1242, 1367
LweI	GCATC	7 cut(s)	190, 516, 727, 1220, 1255, 1331, 1356
MaeI	CTAG	1 cut(s)	701
MaeII	ACGT	1 cut(s)	459
MaeIII	GTNAC	3 cut(s)	275, 625, 1128
MalI	GATC	5 cut(s)	58, 106, 232, 844, 1365
MboI	GATC	5 cut(s)	56, 104, 230, 842, 1363
MboII	GAAGA	2 cut(s)	187, 892
MflI	RGATCY	3 cut(s)	56, 104, 842
MhlI	GDGCHC	3 cut(s)	573, 967, 1077
MlsI	TGGCCA	1 cut(s)	936
MluCI	AATT	8 cut(s)	80, 579, 1009, 1037, 1058, 1112, 1172, 1260
MluNI	TGGCCA	1 cut(s)	936
MmeI	TCCRAC	1 cut(s)	636
MnlI	CCTC	7 cut(s)	447, 595, 654, 690, 769, 829, 1093
Mox20I	TGGCCA	1 cut(s)	936
Mph1103I	ATGCAT	2 cut(s)	205, 961
MscI	TGGCCA	1 cut(s)	936
MseI	TTAA	6 cut(s)	23, 50, 222, 330, 672, 804
Msp20I	TGGCCA	1 cut(s)	936
MspA1I	CMGCKG	2 cut(s)	344, 751
MspCI	CTTAAG	1 cut(s)	329
MspI	CCGG	3 cut(s)	144, 157, 300
MspR9I	CCNGG	5 cut(s)	145, 158, 240, 521, 858
MvaI	CCWGG	3 cut(s)	240, 521, 858
MwoI	GCNNNNNNNGC	6 cut(s)	14, 74, 292, 334, 794, 914
NciI	CCSGG	2 cut(s)	145, 158
NdeI	CATATG	1 cut(s)	526
NdeII	GATC	5 cut(s)	56, 104, 230, 842, 1363
NlaIII	CATG	8 cut(s)	155, 237, 309, 557, 963, 1090, 1301, 1334
NlaIV	GGNNCC	3 cut(s)	96, 349, 1053
NmeAIII	GCCGAG	1 cut(s)	1147
NmuCI	GTSAC	2 cut(s)	275, 625
NsiI	ATGCAT	2 cut(s)	205, 961
NspI	RCATGY	3 cut(s)	155, 309, 963
PciI	ACATGT	1 cut(s)	305
PfeI	GAWTC	4 cut(s)	11, 861, 950, 1294
PkrI	GCNGC	8 cut(s)	7, 150, 288, 336, 709, 790, 1257, 1382
PscI	ACATGT	1 cut(s)	305
Psp124BI	GAGCTC	1 cut(s)	1077
Psp6I	CCWGG	3 cut(s)	238, 519, 856
PspEI	GGTNACC	1 cut(s)	275
PspFI	CCCAGC	2 cut(s)	340, 344
PspGI	CCWGG	3 cut(s)	238, 519, 856
PspN4I	GGNNCC	3 cut(s)	96, 349, 1053
PspPI	GGNCC	4 cut(s)	348, 385, 395, 989
PstI	CTGCAG	1 cut(s)	499
PstNI	CAGNNNCTG	1 cut(s)	1247
PsuI	RGATCY	3 cut(s)	56, 104, 842
PvuII	CAGCTG	2 cut(s)	344, 751
RsaI	GTAC	1 cut(s)	1340
RsaNI	GTAC	1 cut(s)	1339
SacI	GAGCTC	1 cut(s)	1077
SaqAI	TTAA	6 cut(s)	23, 50, 222, 330, 672, 804
SatI	GCNGC	8 cut(s)	6, 149, 287, 335, 708, 789, 1256, 1381
Sau3AI	GATC	5 cut(s)	56, 104, 230, 842, 1363
Sau96I	GGNCC	4 cut(s)	348, 385, 395, 989
ScrFI	CCNGG	5 cut(s)	145, 158, 240, 521, 858
SduI	GDGCHC	3 cut(s)	573, 967, 1077
SfaNI	GCATC	7 cut(s)	190, 516, 727, 1220, 1255, 1331, 1356
SfcI	CTRYAG	1 cut(s)	495
SinI	GGWCC	2 cut(s)	385, 395
SmlI	CTYRAG	3 cut(s)	329, 443, 1076
SmoI	CTYRAG	3 cut(s)	329, 443, 1076
Sse9I	AATT	8 cut(s)	80, 579, 1009, 1037, 1058, 1112, 1172, 1260
SspMI	CTAG	1 cut(s)	701
SstI	GAGCTC	1 cut(s)	1077
StyD4I	CCNGG	5 cut(s)	143, 156, 238, 519, 856
StyI	CCWWGG	1 cut(s)	280
TaaI	ACNGT	5 cut(s)	21, 928, 1094, 1196, 1221
TaiI	ACGT	1 cut(s)	462
TaqI	TCGA	1 cut(s)	1362
TasI	AATT	8 cut(s)	80, 579, 1009, 1037, 1058, 1112, 1172, 1260
TfiI	GAWTC	4 cut(s)	11, 861, 950, 1294
Tru1I	TTAA	6 cut(s)	23, 50, 222, 330, 672, 804
Tru9I	TTAA	6 cut(s)	23, 50, 222, 330, 672, 804
TscAI	CASTG	2 cut(s)	598, 1130
TseFI	GTSAC	2 cut(s)	275, 625
TseI	GCWGC	8 cut(s)	5, 148, 286, 334, 707, 788, 1255, 1380
Tsp45I	GTSAC	2 cut(s)	275, 625
TspDTI	ATGAA	3 cut(s)	455, 1103, 1347
TspGWI	ACGGA	4 cut(s)	69, 408, 461, 1243
TspRI	CASTG	2 cut(s)	598, 1130
Vha464I	CTTAAG	1 cut(s)	329
VneI	GTGCAC	1 cut(s)	569
VpaK11BI	GGWCC	2 cut(s)	385, 395
XapI	RAATTY	4 cut(s)	80, 1009, 1037, 1112
XceI	RCATGY	3 cut(s)	155, 309, 963
XcmI	CCANNNNNNNNNTGG	1 cut(s)	358
XmiI	GTMKAC	1 cut(s)	980
XspI	CTAG	1 cut(s)	701
Zsp2I	ATGCAT	2 cut(s)	205, 961

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.