AT5G62770

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011713
Physical Location & Seq
Forward (+)
25210301 .. 25211665
1365 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G62770.1

Sequence Viewer

Length: 807 bp
ATGCAGACGAGTCGGTTACTCTCCTTCTCCTCTAACTCTCCGAGTTTTGGCAGCTTCTCCTCCGCCGTTGACCTCGCTGCAATCGCCGCTCGAGTCGTCGAAGAATTCAGAGATCACGACCAAACACAATCCGATTCTTCTCCCCACCGCGACGACGATAATGATTCCGACTTCGCTTTCGACTGTCCAAGCAACACGTGTTCTCAGCCTCTCGCTACCGCCGACGAGATTTTCTGTAACGGTCAGATCCGTCCGTTGAATCCGTACGGTGGAAATGCTCCGGTGGAATCTCAACCGACGAGTAAGATTACTACTCTTCCTCCTCGTCGTCGTAGACCGGCGTTGAGGAAACTGATGAGCGAGGATCGAGATCCGGCTTCGAATTCTTCGTCGGAAGCTGAAGAGGATCTTACTGGTGTTCCTCCGGAGACGTACTGTGTATGGAAACCTAAACAATCGAATTCCGGAGATGATGATCTTCAAAGACTTTCGTCTTCTCCGTCACACAGCAAAATCAAAAGCCATTCAGCTGGGTTTTCGAAACGTTGGAAGCTCCGGAATCTTCTGTACGTTAGAAGCAGTAGTGAAGGAAACGATAAGCTCGTCTTTCCGGCGCCGGTTAAGAAGAACGACGAGACGGTCTCCGATCAAAGAGAAGAAGAGGAACCGCCGTCAAAGGTGGACGGAGAGGAAGAAGGAAGGGAAAGGGAAGAGACAAAACGACAGACGTATGTGCCGTATAGAAAGGATATGATTGGAATATTGAAAAATGTGAATGGGCTAAGTCGTCATTTACGTCCTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

268

Amino Acids

29.99

Weight (kDa)

5.53

Isoelectric Point (pI)

74.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 74 - 237 2.6e-20 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011713)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62770
fragaria_vesca FvH4_5g05310
malus_domestica MD14G1180400.v1.1
prunus_persica Prupe.5G173800_v2.0.a1 Prupe.5G173800_v2.0.a1
pyrus_communis pycom06g15510 pycom14g15040
rosa_chinensis RchiOBHm_Chr7g0193551
rosa_laevigata RLG00000004263
rosa_multiflora Rmu_sc0000282.1_g000001 Rmu_sc0041282.1_g000001 Rmu_ssc0000035.1_g000002
rosa_roxburghii Rroxscaffold_3G00262010
rosa_rugosa Rorug07G0010200
rosa_samantha Rh7AG137000 Rh7BG137300 Rh7CG140600 Rh7DG138400
rosa_wichuraiana Rw7G011670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 638
AccB1I GGYRCC 1 cut(s) 613
AccBSI CCGCTC 1 cut(s) 89
AccI GTMKAC 1 cut(s) 334
AccII CGCG 1 cut(s) 150
AccIII TCCGGA 3 cut(s) 424, 464, 555
AciI CCGC 5 cut(s) 63, 87, 148, 219, 668
AclI AACGTT 1 cut(s) 544
AclWI GGATC 4 cut(s) 241, 365, 372, 414
AcsI RAATTY 3 cut(s) 104, 382, 460
AcuI CTGAAG 1 cut(s) 420
AcvI CACGTG 1 cut(s) 198
AcyI GRCGYC 1 cut(s) 614
AfaI GTAC 3 cut(s) 266, 434, 569
AfiI CCNNNNNNNGG 2 cut(s) 47, 269
AflIII ACRYGT 2 cut(s) 195, 197
AgsI TTSAA 3 cut(s) 259, 482, 766
AluBI AGCT 5 cut(s) 54, 398, 530, 553, 601
AluI AGCT 5 cut(s) 54, 398, 530, 553, 601
Alw26I GTCTC 4 cut(s) 422, 629, 646, 707
AlwI GGATC 4 cut(s) 241, 365, 372, 414
Ama87I CYCGRG 1 cut(s) 90
Aor13HI TCCGGA 3 cut(s) 424, 464, 555
ApeKI GCWGC 2 cut(s) 51, 77
ApoI RAATTY 3 cut(s) 104, 382, 460
ArsI GACNNNNNNTTYG 4 cut(s) 161, 193, 374, 406
AspLEI GCGC 1 cut(s) 616
AsuII TTCGAA 2 cut(s) 380, 539
AvaI CYCGRG 1 cut(s) 90
BanI GGYRCC 1 cut(s) 613
BbrPI CACGTG 1 cut(s) 198
BbsI GAAGAC 1 cut(s) 486
BbvI GCAGC 2 cut(s) 63, 64
BceAI ACGGC 3 cut(s) 50, 655, 721
BcgI CGANNNNNNTGC 1 cut(s) 27
BcoDI GTCTC 4 cut(s) 422, 629, 646, 707
BfoI RGCGCY 1 cut(s) 617
BisI GCNGC 3 cut(s) 52, 78, 87
BlsI GCNGC 3 cut(s) 53, 79, 88
BmeT110I CYCGRG 1 cut(s) 90
BmiI GGNNCC 2 cut(s) 615, 666
BoxI GACNNNNGTC 1 cut(s) 490
BpiI GAAGAC 1 cut(s) 486
BplI GAGNNNNNCTC 2 cut(s) 626, 658
Bpu14I TTCGAA 2 cut(s) 380, 539
BsaAI YACGTR 1 cut(s) 198
BsaBI GATNNNNATC 2 cut(s) 369, 474
BsaHI GRCGYC 1 cut(s) 614
BsaI GGTCTC 1 cut(s) 646
BsaWI WCCGGW 4 cut(s) 280, 424, 464, 555
BsaXI ACNNNNNCTCC 2 cut(s) 304, 334
Bsc4I CCNNNNNNNGG 2 cut(s) 47, 269
Bse118I RCCGGY 2 cut(s) 337, 616
Bse1I ACTGG 1 cut(s) 418
Bse8I GATNNNNATC 2 cut(s) 369, 474
BseAI TCCGGA 3 cut(s) 424, 464, 555
BseJI GATNNNNATC 2 cut(s) 369, 474
BseLI CCNNNNNNNGG 2 cut(s) 47, 269
BseMII CTCAG 1 cut(s) 218
BseNI ACTGG 1 cut(s) 418
BseRI GAGGAG 3 cut(s) 19, 49, 312
BseXI GCAGC 2 cut(s) 63, 64
BseYI CCCAGC 1 cut(s) 530
Bsh1236I CGCG 1 cut(s) 150
BshNI GGYRCC 1 cut(s) 613
BsiHKCI CYCGRG 1 cut(s) 90
BsiSI CCGG 8 cut(s) 281, 338, 374, 425, 465, 556, 611, 617
BsiWI CGTACG 1 cut(s) 264
BslI CCNNNNNNNGG 2 cut(s) 47, 269
BsmAI GTCTC 4 cut(s) 422, 629, 646, 707
BsmBI CGTCTC 2 cut(s) 422, 629
Bso31I GGTCTC 1 cut(s) 646
BsoBI CYCGRG 1 cut(s) 90
Bsp119I TTCGAA 2 cut(s) 380, 539
Bsp13I TCCGGA 3 cut(s) 424, 464, 555
Bsp143I GATC 7 cut(s) 112, 246, 364, 370, 406, 475, 646
BspACI CCGC 5 cut(s) 63, 87, 148, 219, 668
BspCNI CTCAG 1 cut(s) 217
BspEI TCCGGA 3 cut(s) 424, 464, 555
BspFNI CGCG 1 cut(s) 150
BspLI GGNNCC 2 cut(s) 615, 666
BspPI GGATC 4 cut(s) 241, 365, 372, 414
BspT104I TTCGAA 2 cut(s) 380, 539
BspT107I GGYRCC 1 cut(s) 613
BspTNI GGTCTC 1 cut(s) 646
BsrBI CCGCTC 1 cut(s) 89
BsrFI RCCGGY 2 cut(s) 337, 616
BsrI ACTGG 1 cut(s) 418
BssAI RCCGGY 2 cut(s) 337, 616
BssMI GATC 7 cut(s) 112, 246, 364, 370, 406, 475, 646
BssNI GRCGYC 1 cut(s) 614
Bst4CI ACNGT 5 cut(s) 185, 242, 269, 437, 640
Bst6I CTCTTC 4 cut(s) 321, 396, 654, 705
BstACI GRCGYC 1 cut(s) 614
BstBAI YACGTR 1 cut(s) 198
BstBI TTCGAA 2 cut(s) 380, 539
BstDEI CTNAG 2 cut(s) 204, 782
BstFNI CGCG 1 cut(s) 150
BstH2I RGCGCY 1 cut(s) 617
BstHHI GCGC 1 cut(s) 616
BstKTI GATC 7 cut(s) 115, 249, 367, 373, 409, 478, 649
BstMAI GTCTC 4 cut(s) 422, 629, 646, 707
BstMBI GATC 7 cut(s) 112, 246, 364, 370, 406, 475, 646
BstMWI GCNNNNNNNGC 2 cut(s) 83, 86
BstPAI GACNNNNGTC 1 cut(s) 490
BstUI CGCG 1 cut(s) 150
BstV1I GCAGC 2 cut(s) 63, 64
BstV2I GAAGAC 1 cut(s) 486
BstX2I RGATCY 3 cut(s) 246, 370, 406
BstXI CCANNNNNNTGG 1 cut(s) 530
BstYI RGATCY 3 cut(s) 246, 370, 406
CfoI GCGC 1 cut(s) 616
Cfr10I RCCGGY 2 cut(s) 337, 616
Csp6I GTAC 3 cut(s) 265, 433, 568
CviJI RGCY 9 cut(s) 54, 208, 377, 398, 522, 530, 553, 601, 781
CviKI_1 RGCY 9 cut(s) 54, 208, 377, 398, 522, 530, 553, 601, 781
CviQI GTAC 3 cut(s) 265, 433, 568
DdeI CTNAG 2 cut(s) 204, 782
DinI GGCGCC 1 cut(s) 615
DpnI GATC 7 cut(s) 114, 248, 366, 372, 408, 477, 648
DpnII GATC 7 cut(s) 112, 246, 364, 370, 406, 475, 646
DrdI GACNNNNNNGTC 1 cut(s) 638
DseDI GACNNNNNNGTC 1 cut(s) 638
Eam1104I CTCTTC 4 cut(s) 321, 396, 654, 705
EarI CTCTTC 4 cut(s) 321, 396, 654, 705
EciI GGCGGA 1 cut(s) 52
Eco31I GGTCTC 1 cut(s) 646
Eco57I CTGAAG 1 cut(s) 420
Eco72I CACGTG 1 cut(s) 198
Eco88I CYCGRG 1 cut(s) 90
EcoRI GAATTC 3 cut(s) 104, 382, 460
EgeI GGCGCC 1 cut(s) 615
EheI GGCGCC 1 cut(s) 615
Esp3I CGTCTC 2 cut(s) 422, 629
FaiI YATR 4 cut(s) 442, 732, 741, 752
FalI AAGNNNNNCTT 4 cut(s) 393, 425, 590, 622
FblI GTMKAC 1 cut(s) 334
Fnu4HI GCNGC 3 cut(s) 52, 78, 87
Fsp4HI GCNGC 3 cut(s) 52, 78, 87
GlaI GCGC 1 cut(s) 615
GluI GCNGC 3 cut(s) 52, 78, 87
GsaI CCCAGC 1 cut(s) 534
HaeII RGCGCY 1 cut(s) 617
HapII CCGG 8 cut(s) 281, 338, 374, 425, 465, 556, 611, 617
HhaI GCGC 1 cut(s) 616
Hin1I GRCGYC 1 cut(s) 614
Hin6I GCGC 1 cut(s) 614
HinP1I GCGC 1 cut(s) 614
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 7 cut(s) 10, 93, 134, 164, 259, 287, 559
HpaII CCGG 8 cut(s) 281, 338, 374, 425, 465, 556, 611, 617
Hpy166II GTNNAC 3 cut(s) 70, 335, 682
Hpy188I TCNGA 7 cut(s) 42, 110, 133, 169, 246, 394, 646
Hpy188III TCNNGA 5 cut(s) 116, 368, 425, 465, 556
Hpy8I GTNNAC 3 cut(s) 70, 335, 682
Hpy99I CGWCG 9 cut(s) 101, 155, 158, 227, 301, 330, 333, 394, 635
HpyAV CCTTC 4 cut(s) 34, 581, 689, 693
HpyCH4III ACNGT 5 cut(s) 185, 242, 269, 437, 640
HpyCH4IV ACGT 6 cut(s) 197, 431, 544, 570, 728, 796
HpyCH4V TGCA 2 cut(s) 4, 80
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 86
HpyF3I CTNAG 2 cut(s) 204, 782
HpySE526I ACGT 6 cut(s) 197, 431, 544, 570, 728, 796
Hsp92I GRCGYC 1 cut(s) 614
HspAI GCGC 1 cut(s) 614
KasI GGCGCC 1 cut(s) 613
Kpn2I TCCGGA 3 cut(s) 424, 464, 555
Kzo9I GATC 7 cut(s) 112, 246, 364, 370, 406, 475, 646
LmnI GCTCC 2 cut(s) 283, 558
Lsp1109I GCAGC 2 cut(s) 63, 64
MaeII ACGT 6 cut(s) 197, 431, 544, 570, 728, 796
MaeIII GTNAC 3 cut(s) 15, 236, 501
MalI GATC 7 cut(s) 114, 248, 366, 372, 408, 477, 648
MbiI CCGCTC 1 cut(s) 89
MboI GATC 7 cut(s) 112, 246, 364, 370, 406, 475, 646
MflI RGATCY 3 cut(s) 246, 370, 406
MluCI AATT 3 cut(s) 104, 382, 460
Mly113I GGCGCC 1 cut(s) 614
MlyI GAGTC 2 cut(s) 19, 102
MmeI TCCRAC 3 cut(s) 192, 372, 527
MroI TCCGGA 3 cut(s) 424, 464, 555
MseI TTAA 1 cut(s) 621
MspA1I CMGCKG 1 cut(s) 530
MspI CCGG 8 cut(s) 281, 338, 374, 425, 465, 556, 611, 617
MvnI CGCG 1 cut(s) 150
MwoI GCNNNNNNNGC 2 cut(s) 83, 86
NarI GGCGCC 1 cut(s) 614
NdeII GATC 7 cut(s) 112, 246, 364, 370, 406, 475, 646
NlaIV GGNNCC 2 cut(s) 615, 666
NmuCI GTSAC 1 cut(s) 501
NspV TTCGAA 2 cut(s) 380, 539
PaeR7I CTCGAG 1 cut(s) 90
PcsI WCGNNNNNNNCGW 6 cut(s) 219, 386, 497, 600, 734, 793
PfeI GAWTC 5 cut(s) 134, 164, 259, 287, 559
Pfl23II CGTACG 1 cut(s) 264
PkrI GCNGC 3 cut(s) 53, 79, 88
PleI GAGTC 2 cut(s) 18, 101
PluTI GGCGCC 1 cut(s) 617
PmaCI CACGTG 1 cut(s) 198
PmlI CACGTG 1 cut(s) 198
PpsI GAGTC 2 cut(s) 18, 101
Ppu21I YACGTR 1 cut(s) 198
PshAI GACNNNNGTC 1 cut(s) 490
Psp1406I AACGTT 1 cut(s) 544
PspCI CACGTG 1 cut(s) 198
PspFI CCCAGC 1 cut(s) 530
PspLI CGTACG 1 cut(s) 264
PspN4I GGNNCC 2 cut(s) 615, 666
PspXI VCTCGAGB 1 cut(s) 90
PsuI RGATCY 3 cut(s) 246, 370, 406
PvuII CAGCTG 1 cut(s) 530
RsaI GTAC 3 cut(s) 266, 434, 569
RsaNI GTAC 3 cut(s) 265, 433, 568
SaqAI TTAA 1 cut(s) 621
SatI GCNGC 3 cut(s) 52, 78, 87
Sau3AI GATC 7 cut(s) 112, 246, 364, 370, 406, 475, 646
SchI GAGTC 2 cut(s) 19, 102
SfoI GGCGCC 1 cut(s) 615
Sfr274I CTCGAG 1 cut(s) 90
SfuI TTCGAA 2 cut(s) 380, 539
SlaI CTCGAG 1 cut(s) 90
SmlI CTYRAG 1 cut(s) 90
SmoI CTYRAG 1 cut(s) 90
Sse9I AATT 3 cut(s) 104, 382, 460
SsiI CCGC 5 cut(s) 63, 87, 148, 219, 668
SspDI GGCGCC 1 cut(s) 613
SspI AATATT 1 cut(s) 762
TaaI ACNGT 5 cut(s) 185, 242, 269, 437, 640
TaiI ACGT 6 cut(s) 200, 434, 547, 573, 731, 799
TaqI TCGA 7 cut(s) 91, 99, 180, 367, 380, 458, 539
TasI AATT 3 cut(s) 104, 382, 460
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 5 cut(s) 134, 164, 259, 287, 559
Tru1I TTAA 1 cut(s) 621
Tru9I TTAA 1 cut(s) 621
TseFI GTSAC 1 cut(s) 501
TseI GCWGC 2 cut(s) 51, 77
Tsp45I GTSAC 1 cut(s) 501
TspGWI ACGGA 5 cut(s) 239, 243, 252, 489, 699
XapI RAATTY 3 cut(s) 104, 382, 460
XhoI CTCGAG 1 cut(s) 90
XmiI GTMKAC 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.