MD14G1180400.v1.1

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011713
Physical Location & Seq
Forward (+)
27307288 .. 27308205
918 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1180400.v1.1.491

Sequence Viewer

Length: 918 bp
ATGCAAGCTGCTGCCACAGGTCCGTTGATCTCGCTCTCCCCCAGCTTCAACACTCACTCTTCCTCCGACAGACTCGCCCAGATCGCCGCCCGAGTCGTTGACGAGTTCTCCCACCAAGACCACCAACTCACCGACGAGTCCGACTGGGAAACTCTATACGATCACCAGGAAGAAGAAAAACTCCACCACCGCCAACAAACCAGAGACGACAACGTACAGAAAGACAAGGAGGAGGAAGAGGAGGAGTTTGAGTTTTCTTTTGTCAGCAGCGGAGGAGAGCCGCACACGTCGCACGTCTCAGCCGACGAGATTTTCTCCAACGGTCAAATCAAACCCACCGTCTACCCTCTCTTCAACCAAGACTTATTGTTACCTCACAACGACGTCGTATCGTCTAAAAATGCTAACGGCAATGTGGATGACGCCGTTAAGAAGCCGAAACAACGTCGTCCACCGCTCAGAATGCTCATGAACGAAGACGAGCGCGAAACGCCGTCCTGCTCGTCATCCGAGGCCGACGACCTCGAGAACCTGCCACCTGACACTTACTGCGTGTGGGCCCCCCCGAAACCGTCGCCTGGACAGCGCGGCTGCAATAAGAGCAGCTCCGCCGGCTCGTCCTCGAAGCGGTGGAAGTTTAGGAACCTTCTTTACCGGAGCAGCAGCGAAGGCAAGGACACTTTCGCCTTTTTGGCTCCCTCGTCGACCAAGACAGCCGAAAACAACAAGGGAGATAACGTCACGCGGAAAAGAGTCGACGGCGGCGGCGGCATGTCGGAGAATGGAGAGCGTTATGTCGTAAGGAGCAAGACTGCGGAGGAAGGAGATAAACGACGGTCGTTTATACCGTACAGGCAGGACTTTGTGGGGTTTTTCTCGAATGTGAATGGGCTTAGCAGGAATTTGCATCCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

306

Amino Acids

34.28

Weight (kDa)

5.45

Isoelectric Point (pI)

57.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 101 - 283 1e-25 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011713)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62770
fragaria_vesca FvH4_5g05310
malus_domestica MD14G1180400.v1.1
prunus_persica Prupe.5G173800_v2.0.a1 Prupe.5G173800_v2.0.a1
pyrus_communis pycom06g15510 pycom14g15040
rosa_chinensis RchiOBHm_Chr7g0193551
rosa_laevigata RLG00000004263
rosa_multiflora Rmu_sc0000282.1_g000001 Rmu_sc0041282.1_g000001 Rmu_ssc0000035.1_g000002
rosa_roxburghii Rroxscaffold_3G00262010
rosa_rugosa Rorug07G0010200
rosa_samantha Rh7AG137000 Rh7BG137300 Rh7CG140600 Rh7DG138400
rosa_wichuraiana Rw7G011670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 387
Acc36I ACCTGC 1 cut(s) 540
AccBSI CCGCTC 1 cut(s) 457
AccI GTMKAC 3 cut(s) 342, 704, 756
AccII CGCG 3 cut(s) 486, 588, 745
AcsI RAATTY 1 cut(s) 901
AcyI GRCGYC 2 cut(s) 384, 423
AfaI GTAC 2 cut(s) 216, 851
AfiI CCNNNNNNNGG 2 cut(s) 578, 627
AflIII ACRYGT 1 cut(s) 285
AgsI TTSAA 2 cut(s) 49, 355
AjiI CACGTC 2 cut(s) 288, 295
AjnI CCWGG 2 cut(s) 165, 577
AluBI AGCT 3 cut(s) 8, 45, 606
AluI AGCT 3 cut(s) 8, 45, 606
Alw26I GTCTC 2 cut(s) 198, 301
Ama87I CYCGRG 2 cut(s) 90, 524
AoxI GGCC 2 cut(s) 513, 558
ApaI GGGCCC 1 cut(s) 562
ApeKI GCWGC 7 cut(s) 8, 11, 267, 591, 603, 660, 663
ApoI RAATTY 1 cut(s) 901
ArsI GACNNNNNNTTYG 2 cut(s) 324, 356
AspLEI GCGC 2 cut(s) 486, 588
AspS9I GGNCC 3 cut(s) 20, 558, 559
AsuHPI GGTGA 2 cut(s) 121, 155
AvaI CYCGRG 2 cut(s) 90, 524
AvaII GGWCC 1 cut(s) 20
BaeGI GKGCMC 1 cut(s) 562
BanII GRGCYC 1 cut(s) 562
BbsI GAAGAC 1 cut(s) 483
BbvI GCAGC 5 cut(s) 279, 578, 615, 672, 675
BceAI ACGGC 4 cut(s) 410, 424, 478, 775
BciT130I CCWGG 2 cut(s) 167, 579
BcoDI GTCTC 2 cut(s) 198, 301
BfuAI ACCTGC 1 cut(s) 540
BglI GCCNNNNNGGC 1 cut(s) 692
BlpI GCTNAGC 1 cut(s) 893
Bme1390I CCNGG 2 cut(s) 167, 579
Bme18I GGWCC 1 cut(s) 20
BmeT110I CYCGRG 2 cut(s) 90, 524
BmgBI CACGTC 2 cut(s) 288, 295
BmgT120I GGNCC 3 cut(s) 20, 558, 559
BmiI GGNNCC 4 cut(s) 560, 561, 644, 696
BmrFI CCNGG 2 cut(s) 167, 579
BmrI ACTGGG 1 cut(s) 154
BmuI ACTGGG 1 cut(s) 154
BpiI GAAGAC 1 cut(s) 483
BplI GAGNNNNNCTC 2 cut(s) 299, 331
Bpu1102I GCTNAGC 1 cut(s) 893
BsaHI GRCGYC 2 cut(s) 384, 423
BsaJI CCNNGG 1 cut(s) 510
BsaWI WCCGGW 1 cut(s) 654
BsaXI ACNNNNNCTCC 8 cut(s) 47, 77, 92, 122, 236, 266, 723, 753
Bsc4I CCNNNNNNNGG 2 cut(s) 578, 627
Bse118I RCCGGY 1 cut(s) 611
Bse1I ACTGG 1 cut(s) 149
Bse3DI GCAATG 1 cut(s) 418
BseBI CCWGG 2 cut(s) 167, 579
BseDI CCNNGG 1 cut(s) 510
BseGI GGATG 3 cut(s) 424, 506, 907
BseLI CCNNNNNNNGG 2 cut(s) 578, 627
BseMI GCAATG 1 cut(s) 418
BseMII CTCAG 2 cut(s) 312, 472
BseNI ACTGG 1 cut(s) 149
BseRI GAGGAG 4 cut(s) 245, 254, 257, 288
BseSI GKGCMC 1 cut(s) 562
BseXI GCAGC 5 cut(s) 279, 578, 615, 672, 675
BseYI CCCAGC 1 cut(s) 41
Bsh1236I CGCG 3 cut(s) 486, 588, 745
Bsh1285I CGRYCG 1 cut(s) 839
BshFI GGCC 2 cut(s) 515, 560
BsiEI CGRYCG 1 cut(s) 839
BsiHKCI CYCGRG 2 cut(s) 90, 524
BsiSI CCGG 2 cut(s) 612, 655
BslI CCNNNNNNNGG 2 cut(s) 578, 627
BsmAI GTCTC 2 cut(s) 198, 301
BsmBI CGTCTC 2 cut(s) 198, 301
BsmI GAATGC 1 cut(s) 468
BsnI GGCC 2 cut(s) 515, 560
BsoBI CYCGRG 2 cut(s) 90, 524
Bsp120I GGGCCC 1 cut(s) 558
Bsp1286I GDGCHC 1 cut(s) 562
Bsp143I GATC 3 cut(s) 27, 81, 160
Bsp1720I GCTNAGC 1 cut(s) 893
BspANI GGCC 2 cut(s) 515, 560
BspCNI CTCAG 2 cut(s) 311, 471
BspFNI CGCG 3 cut(s) 486, 588, 745
BspHI TCATGA 1 cut(s) 468
BspLI GGNNCC 4 cut(s) 560, 561, 644, 696
BspMI ACCTGC 1 cut(s) 540
BsrBI CCGCTC 1 cut(s) 457
BsrDI GCAATG 1 cut(s) 418
BsrFI RCCGGY 1 cut(s) 611
BsrI ACTGG 1 cut(s) 149
BssAI RCCGGY 1 cut(s) 611
BssECI CCNNGG 1 cut(s) 510
BssMI GATC 3 cut(s) 27, 81, 160
BssNI GRCGYC 2 cut(s) 384, 423
Bst2UI CCWGG 2 cut(s) 167, 579
Bst4CI ACNGT 5 cut(s) 323, 340, 573, 837, 849
Bst6I CTCTTC 3 cut(s) 64, 231, 356
BstACI GRCGYC 2 cut(s) 384, 423
BstC8I GCNNGC 2 cut(s) 6, 613
BstDEI CTNAG 3 cut(s) 298, 458, 893
BstF5I GGATG 3 cut(s) 424, 506, 907
BstFNI CGCG 3 cut(s) 486, 588, 745
BstHHI GCGC 2 cut(s) 486, 588
BstKTI GATC 3 cut(s) 30, 84, 163
BstMAI GTCTC 2 cut(s) 198, 301
BstMBI GATC 3 cut(s) 27, 81, 160
BstMCI CGRYCG 1 cut(s) 839
BstNI CCWGG 2 cut(s) 167, 579
BstNSI RCATGY 1 cut(s) 775
BstSCI CCNGG 2 cut(s) 165, 577
BstSLI GKGCMC 1 cut(s) 562
BstUI CGCG 3 cut(s) 486, 588, 745
BstV1I GCAGC 5 cut(s) 279, 578, 615, 672, 675
BstV2I GAAGAC 1 cut(s) 483
BsuRI GGCC 2 cut(s) 515, 560
BtrI CACGTC 2 cut(s) 288, 295
BtsCI GGATG 3 cut(s) 424, 506, 907
BveI ACCTGC 1 cut(s) 540
Cac8I GCNNGC 2 cut(s) 6, 613
CciI TCATGA 1 cut(s) 468
CfoI GCGC 2 cut(s) 486, 588
Cfr10I RCCGGY 1 cut(s) 611
Cfr13I GGNCC 3 cut(s) 20, 558, 559
CseI GACGC 1 cut(s) 431
Csp6I GTAC 2 cut(s) 215, 850
CviAII CATG 2 cut(s) 469, 772
CviQI GTAC 2 cut(s) 215, 850
DdeI CTNAG 3 cut(s) 298, 458, 893
DpnI GATC 3 cut(s) 29, 83, 162
DpnII GATC 3 cut(s) 27, 81, 160
Eam1104I CTCTTC 3 cut(s) 64, 231, 356
EarI CTCTTC 3 cut(s) 64, 231, 356
EciI GGCGGA 1 cut(s) 598
Eco24I GRGCYC 1 cut(s) 562
Eco47I GGWCC 1 cut(s) 20
Eco88I CYCGRG 2 cut(s) 90, 524
EcoO109I RGGNCCY 1 cut(s) 559
EcoRII CCWGG 2 cut(s) 165, 577
EcoT38I GRGCYC 1 cut(s) 562
Esp3I CGTCTC 2 cut(s) 198, 301
FaeI CATG 2 cut(s) 472, 775
FaiI YATR 5 cut(s) 157, 470, 773, 795, 845
FatI CATG 2 cut(s) 468, 771
FblI GTMKAC 3 cut(s) 342, 704, 756
FokI GGATG 3 cut(s) 431, 493, 894
FriOI GRGCYC 1 cut(s) 562
GlaI GCGC 2 cut(s) 485, 587
GsaI CCCAGC 1 cut(s) 45
HaeIII GGCC 2 cut(s) 515, 560
HapII CCGG 2 cut(s) 612, 655
HgaI GACGC 1 cut(s) 431
HhaI GCGC 2 cut(s) 486, 588
Hin1I GRCGYC 2 cut(s) 384, 423
Hin1II CATG 2 cut(s) 472, 775
Hin6I GCGC 2 cut(s) 484, 586
HinP1I GCGC 2 cut(s) 484, 586
HincII GTYRAC 3 cut(s) 100, 705, 757
HindII GTYRAC 3 cut(s) 100, 705, 757
HinfI GANTC 4 cut(s) 72, 93, 137, 753
HpaII CCGG 2 cut(s) 612, 655
HphI GGTGA 2 cut(s) 121, 155
Hpy166II GTNNAC 5 cut(s) 100, 343, 452, 705, 757
Hpy188I TCNGA 5 cut(s) 67, 142, 461, 511, 778
Hpy188III TCNNGA 3 cut(s) 469, 526, 877
Hpy8I GTNNAC 5 cut(s) 100, 343, 452, 705, 757
HpyAV CCTTC 3 cut(s) 656, 662, 815
HpyCH4III ACNGT 5 cut(s) 323, 340, 573, 837, 849
HpyCH4IV ACGT 6 cut(s) 213, 287, 294, 384, 445, 738
HpyCH4V TGCA 3 cut(s) 4, 594, 907
HpyF3I CTNAG 3 cut(s) 298, 458, 893
HpySE526I ACGT 6 cut(s) 213, 287, 294, 384, 445, 738
Hsp92I GRCGYC 2 cut(s) 384, 423
Hsp92II CATG 2 cut(s) 472, 775
HspAI GCGC 2 cut(s) 484, 586
KroI GCCGGC 1 cut(s) 611
KroNI GCCGGC 1 cut(s) 613
Kzo9I GATC 3 cut(s) 27, 81, 160
LmnI GCTCC 4 cut(s) 611, 657, 700, 804
Lsp1109I GCAGC 5 cut(s) 279, 578, 615, 672, 675
MaeII ACGT 6 cut(s) 213, 287, 294, 384, 445, 738
MaeIII GTNAC 2 cut(s) 369, 739
MalI GATC 3 cut(s) 29, 83, 162
MbiI CCGCTC 1 cut(s) 457
MboI GATC 3 cut(s) 27, 81, 160
MboII GAAGA 6 cut(s) 51, 182, 185, 248, 343, 488
MhlI GDGCHC 1 cut(s) 562
MluCI AATT 1 cut(s) 901
MlyI GAGTC 4 cut(s) 66, 102, 146, 762
MmeI TCCRAC 4 cut(s) 90, 165, 342, 756
MroNI GCCGGC 1 cut(s) 611
MseI TTAA 1 cut(s) 429
MspA1I CMGCKG 1 cut(s) 270
MspI CCGG 2 cut(s) 612, 655
MspR9I CCNGG 2 cut(s) 167, 579
Mva1269I GAATGC 1 cut(s) 468
MvaI CCWGG 2 cut(s) 167, 579
MvnI CGCG 3 cut(s) 486, 588, 745
NaeI GCCGGC 1 cut(s) 613
NdeII GATC 3 cut(s) 27, 81, 160
NgoMIV GCCGGC 1 cut(s) 611
NlaIII CATG 2 cut(s) 472, 775
NlaIV GGNNCC 4 cut(s) 560, 561, 644, 696
NmuCI GTSAC 1 cut(s) 739
NspI RCATGY 1 cut(s) 775
PaeR7I CTCGAG 1 cut(s) 524
PagI TCATGA 1 cut(s) 468
PcsI WCGNNNNNNNCGW 2 cut(s) 300, 845
PctI GAATGC 1 cut(s) 468
PdiI GCCGGC 1 cut(s) 613
PleI GAGTC 4 cut(s) 66, 101, 145, 761
PpsI GAGTC 4 cut(s) 66, 101, 145, 761
Psp6I CCWGG 2 cut(s) 165, 577
PspFI CCCAGC 1 cut(s) 41
PspGI CCWGG 2 cut(s) 165, 577
PspN4I GGNNCC 4 cut(s) 560, 561, 644, 696
PspOMI GGGCCC 1 cut(s) 558
PspPI GGNCC 3 cut(s) 20, 558, 559
PsrI GAACNNNNNNTAC 2 cut(s) 635, 667
RsaI GTAC 2 cut(s) 216, 851
RsaNI GTAC 2 cut(s) 215, 850
SalI GTCGAC 2 cut(s) 703, 755
SaqAI TTAA 1 cut(s) 429
Sau3AI GATC 3 cut(s) 27, 81, 160
Sau96I GGNCC 3 cut(s) 20, 558, 559
SchI GAGTC 4 cut(s) 66, 102, 146, 762
ScrFI CCNGG 2 cut(s) 167, 579
SduI GDGCHC 1 cut(s) 562
Sfr274I CTCGAG 1 cut(s) 524
SinI GGWCC 1 cut(s) 20
SlaI CTCGAG 1 cut(s) 524
SmlI CTYRAG 1 cut(s) 524
SmoI CTYRAG 1 cut(s) 524
Sse9I AATT 1 cut(s) 901
StyD4I CCNGG 2 cut(s) 165, 577
TaaI ACNGT 5 cut(s) 323, 340, 573, 837, 849
TaiI ACGT 6 cut(s) 216, 290, 297, 387, 448, 741
TaqI TCGA 5 cut(s) 525, 623, 704, 756, 878
TasI AATT 1 cut(s) 901
TauI GCSGC 6 cut(s) 89, 283, 591, 765, 768, 771
Tru1I TTAA 1 cut(s) 429
Tru9I TTAA 1 cut(s) 429
TseFI GTSAC 1 cut(s) 739
TseI GCWGC 7 cut(s) 8, 11, 267, 591, 603, 660, 663
Tsp45I GTSAC 1 cut(s) 739
TspDTI ATGAA 1 cut(s) 485
TspGWI ACGGA 1 cut(s) 12
VpaK11BI GGWCC 1 cut(s) 20
XapI RAATTY 1 cut(s) 901
XceI RCATGY 1 cut(s) 775
XhoI CTCGAG 1 cut(s) 524
XmiI GTMKAC 3 cut(s) 342, 704, 756
ZraI GACGTC 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.