FvH4_5g05310

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011713
Physical Location & Seq
Reverse (-)
3103608 .. 3104950
1343 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g05310.t1

Sequence Viewer

Length: 921 bp
ATGCAGAGCGGCGGTGCTGCTATCGGAGGTTCGTTAATATCGTTATCTCCGAGCTTCAACGCGTATTCTTCCTCCGACAGCCTCGCTCAGATCGCCGCCAGAGTCGTCAGCGAGTTTCGTCAGGAGCTTGACGAGGCTGGTTTGGAGGATATTGATGACGTGTATGAGAACAACGGCGGGCAATTCTCACCGCCGCGGTTGGAGCAAGAAGCGCGAAACGACGACGACGTAAAGGTGGTGGAAGATGATGATGATGATGAGGAGGAGGAGGAGTTTGAGTTCGCTTTTGTATGCAGAGACGCGCCGACGTCGCCGATAGCGGCGGAGGAGGTGTTCTACAACGGCCAGATCCGGCCGGTTTACCCCGTCTTCGACCAGAGCTTGCTTCTACAAAACGACGACGACGTGGATGCCGTGAAGAAAGATTCCGAGAAGTCCGCGGCGGCGGAGGCGGAGGCGGTAACGAAGCCGCGTCGTCGGCCGCTAAGAATGCTCATGATCGAGGAGGAGCGCGAGACGGCGTCGTGCTCGTCCTCCGAGGCCGACGACCTCGAGAGCCTGCCACCTGGCACTTACTGCGTGTGGGCCCCGCCCAAGGCTGATTCGTCTTCCGAGAAGGGTGGGAAAATTAGCGGCGGCGGTTGCGGGAAGAGCGGCTCGACCGGGTCGTCGAAGAGGTGGAAGTTTCGGGACCTTCTTTCTCGGAGCAGCAGCGACGGCTCCAAGGACAATTTGGTCTTTTTCGCACCCAAGGCCGCGGAGAAGGAGGGCGGTAACGTCGTGGTCGTCCGGAAAGTGAGGCGCAGCGGAAAAGGCGCGGCTGATGGGGATAAACGACGGTCGTTTTTGCCGTACAAGCAGGACCTGGTGGGGTTTTTCTCTAACGTCAATGGGCTTAGCAGGAATTTGCATCCATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

307

Amino Acids

33.29

Weight (kDa)

4.6

Isoelectric Point (pI)

65.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 108 - 264 4.4e-23 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011713)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62770
fragaria_vesca FvH4_5g05310
malus_domestica MD14G1180400.v1.1
prunus_persica Prupe.5G173800_v2.0.a1 Prupe.5G173800_v2.0.a1
pyrus_communis pycom06g15510 pycom14g15040
rosa_chinensis RchiOBHm_Chr7g0193551
rosa_laevigata RLG00000004263
rosa_multiflora Rmu_sc0000282.1_g000001 Rmu_sc0041282.1_g000001 Rmu_ssc0000035.1_g000002
rosa_roxburghii Rroxscaffold_3G00262010
rosa_rugosa Rorug07G0010200
rosa_samantha Rh7AG137000 Rh7BG137300 Rh7CG140600 Rh7DG138400
rosa_wichuraiana Rw7G011670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 667, 734
AatII GACGTC 1 cut(s) 311
AccBSI CCGCTC 2 cut(s) 9, 654
AccII CGCG 9 cut(s) 62, 196, 214, 302, 440, 472, 513, 758, 818
AccIII TCCGGA 1 cut(s) 789
AclWI GGATC 1 cut(s) 343
AcoI YGGCCR 3 cut(s) 343, 353, 479
AcsI RAATTY 1 cut(s) 904
AcyI GRCGYC 2 cut(s) 308, 521
AfaI GTAC 1 cut(s) 854
AfiI CCNNNNNNNGG 1 cut(s) 595
AflIII ACRYGT 2 cut(s) 60, 159
AgsI TTSAA 1 cut(s) 58
AjiI CACGTC 2 cut(s) 160, 406
AjnI CCWGG 2 cut(s) 565, 864
AloI GAACNNNNNNTCC 4 cut(s) 263, 295, 317, 349
AluBI AGCT 3 cut(s) 54, 127, 381
AluI AGCT 3 cut(s) 54, 127, 381
Alw21I GWGCWC 1 cut(s) 530
Alw26I GTCTC 2 cut(s) 291, 509
AlwI GGATC 1 cut(s) 343
AlwNI CAGNNNCTG 1 cut(s) 865
Ama87I CYCGRG 1 cut(s) 551
Aor13HI TCCGGA 1 cut(s) 789
AoxI GGCC 6 cut(s) 343, 353, 479, 540, 585, 753
ApaI GGGCCC 1 cut(s) 589
ApeKI GCWGC 4 cut(s) 17, 708, 711, 804
ApoI RAATTY 1 cut(s) 904
AspLEI GCGC 5 cut(s) 214, 304, 513, 804, 818
AspS9I GGNCC 4 cut(s) 585, 586, 691, 862
AsuC2I CCSGG 1 cut(s) 664
AsuHPI GGTGA 1 cut(s) 180
AvaI CYCGRG 1 cut(s) 551
AvaII GGWCC 2 cut(s) 691, 862
BaeGI GKGCMC 1 cut(s) 589
BanII GRGCYC 1 cut(s) 589
BbsI GAAGAC 2 cut(s) 361, 600
Bbv12I GWGCWC 1 cut(s) 530
BbvI GCAGC 4 cut(s) 4, 720, 723, 816
BccI CCATC 1 cut(s) 818
BceAI ACGGC 6 cut(s) 190, 358, 398, 534, 733, 835
BcgI CGANNNNNNTGC 2 cut(s) 392, 426
BciT130I CCWGG 2 cut(s) 567, 866
BcnI CCSGG 1 cut(s) 664
BcoDI GTCTC 2 cut(s) 291, 509
BlpI GCTNAGC 1 cut(s) 896
Bme1390I CCNGG 3 cut(s) 567, 664, 866
Bme18I GGWCC 2 cut(s) 691, 862
BmeT110I CYCGRG 1 cut(s) 551
BmgBI CACGTC 2 cut(s) 160, 406
BmgT120I GGNCC 4 cut(s) 585, 586, 691, 862
BmiI GGNNCC 4 cut(s) 587, 588, 692, 721
BmrFI CCNGG 3 cut(s) 567, 664, 866
BmsI GCATC 1 cut(s) 400
BpiI GAAGAC 2 cut(s) 361, 600
Bpu1102I GCTNAGC 1 cut(s) 896
BpuMI CCSGG 1 cut(s) 664
BsaHI GRCGYC 2 cut(s) 308, 521
BsaJI CCNNGG 7 cut(s) 194, 438, 537, 594, 723, 750, 756
BsaWI WCCGGW 1 cut(s) 789
BsaXI ACNNNNNCTCC 6 cut(s) 257, 263, 287, 293, 317, 347
Bsc4I CCNNNNNNNGG 1 cut(s) 595
Bse118I RCCGGY 1 cut(s) 355
BseAI TCCGGA 1 cut(s) 789
BseBI CCWGG 2 cut(s) 567, 866
BseDI CCNNGG 7 cut(s) 194, 438, 537, 594, 723, 750, 756
BseGI GGATG 2 cut(s) 415, 910
BseLI CCNNNNNNNGG 1 cut(s) 595
BseMII CTCAG 1 cut(s) 101
BseRI GAGGAG 7 cut(s) 275, 278, 281, 284, 341, 518, 521
BseSI GKGCMC 1 cut(s) 589
BseX3I CGGCCG 2 cut(s) 353, 479
BseXI GCAGC 4 cut(s) 4, 720, 723, 816
Bsh1236I CGCG 9 cut(s) 62, 196, 214, 302, 440, 472, 513, 758, 818
Bsh1285I CGRYCG 4 cut(s) 356, 482, 663, 842
BshFI GGCC 6 cut(s) 345, 355, 481, 542, 587, 755
BsiEI CGRYCG 4 cut(s) 356, 482, 663, 842
BsiHKAI GWGCWC 1 cut(s) 530
BsiHKCI CYCGRG 1 cut(s) 551
BsiSI CCGG 4 cut(s) 352, 356, 663, 790
BslFI GGGAC 1 cut(s) 704
BslI CCNNNNNNNGG 1 cut(s) 595
BsmAI GTCTC 2 cut(s) 291, 509
BsmBI CGTCTC 2 cut(s) 291, 509
BsmFI GGGAC 1 cut(s) 704
BsmI GAATGC 1 cut(s) 495
BsnI GGCC 6 cut(s) 345, 355, 481, 542, 587, 755
BsoBI CYCGRG 1 cut(s) 551
Bsp120I GGGCCC 1 cut(s) 585
Bsp1286I GDGCHC 2 cut(s) 530, 589
Bsp13I TCCGGA 1 cut(s) 789
Bsp143I GATC 3 cut(s) 90, 348, 498
Bsp1720I GCTNAGC 1 cut(s) 896
BspANI GGCC 6 cut(s) 345, 355, 481, 542, 587, 755
BspCNI CTCAG 1 cut(s) 100
BspEI TCCGGA 1 cut(s) 789
BspFNI CGCG 9 cut(s) 62, 196, 214, 302, 440, 472, 513, 758, 818
BspHI TCATGA 1 cut(s) 495
BspLI GGNNCC 4 cut(s) 587, 588, 692, 721
BspPI GGATC 1 cut(s) 343
BspQI GCTCTTC 1 cut(s) 644
BsrBI CCGCTC 2 cut(s) 9, 654
BsrFI RCCGGY 1 cut(s) 355
BssAI RCCGGY 1 cut(s) 355
BssECI CCNNGG 7 cut(s) 194, 438, 537, 594, 723, 750, 756
BssMI GATC 3 cut(s) 90, 348, 498
BssNI GRCGYC 2 cut(s) 308, 521
BssT1I CCWWGG 3 cut(s) 594, 723, 750
Bst2UI CCWGG 2 cut(s) 567, 866
Bst4CI ACNGT 1 cut(s) 840
Bst6I CTCTTC 2 cut(s) 644, 668
BstACI GRCGYC 2 cut(s) 308, 521
BstAPI GCANNNNNTGC 1 cut(s) 576
BstC8I GCNNGC 3 cut(s) 179, 383, 560
BstDEI CTNAG 3 cut(s) 87, 485, 896
BstDSI CCRYGG 3 cut(s) 194, 438, 756
BstF5I GGATG 2 cut(s) 415, 910
BstFNI CGCG 9 cut(s) 62, 196, 214, 302, 440, 472, 513, 758, 818
BstHHI GCGC 5 cut(s) 214, 304, 513, 804, 818
BstKTI GATC 3 cut(s) 93, 351, 501
BstMAI GTCTC 2 cut(s) 291, 509
BstMBI GATC 3 cut(s) 90, 348, 498
BstMCI CGRYCG 4 cut(s) 356, 482, 663, 842
BstNI CCWGG 2 cut(s) 567, 866
BstSCI CCNGG 3 cut(s) 565, 662, 864
BstSLI GKGCMC 1 cut(s) 589
BstUI CGCG 9 cut(s) 62, 196, 214, 302, 440, 472, 513, 758, 818
BstV1I GCAGC 4 cut(s) 4, 720, 723, 816
BstV2I GAAGAC 2 cut(s) 361, 600
BstX2I RGATCY 1 cut(s) 348
BstYI RGATCY 1 cut(s) 348
BstZI CGGCCG 2 cut(s) 353, 479
BsuRI GGCC 6 cut(s) 345, 355, 481, 542, 587, 755
BtgI CCRYGG 3 cut(s) 194, 438, 756
BtrI CACGTC 2 cut(s) 160, 406
BtsCI GGATG 2 cut(s) 415, 910
Cac8I GCNNGC 3 cut(s) 179, 383, 560
CaiI CAGNNNCTG 1 cut(s) 865
CciI TCATGA 1 cut(s) 495
CfoI GCGC 5 cut(s) 214, 304, 513, 804, 818
Cfr10I RCCGGY 1 cut(s) 355
Cfr13I GGNCC 4 cut(s) 585, 586, 691, 862
Cfr42I CCGCGG 3 cut(s) 197, 441, 759
CseI GACGC 3 cut(s) 308, 461, 510
CsiI ACCWGGT 1 cut(s) 864
Csp6I GTAC 1 cut(s) 853
CviAII CATG 1 cut(s) 496
CviQI GTAC 1 cut(s) 853
DdeI CTNAG 3 cut(s) 87, 485, 896
DpnI GATC 3 cut(s) 92, 350, 500
DpnII GATC 3 cut(s) 90, 348, 498
DrdI GACNNNNNNGTC 2 cut(s) 667, 734
DseDI GACNNNNNNGTC 2 cut(s) 667, 734
EaeI YGGCCR 3 cut(s) 343, 353, 479
EagI CGGCCG 2 cut(s) 353, 479
Eam1104I CTCTTC 2 cut(s) 644, 668
EarI CTCTTC 2 cut(s) 644, 668
EciI GGCGGA 3 cut(s) 338, 461, 467
EclXI CGGCCG 2 cut(s) 353, 479
Eco130I CCWWGG 3 cut(s) 594, 723, 750
Eco24I GRGCYC 1 cut(s) 589
Eco47I GGWCC 2 cut(s) 691, 862
Eco52I CGGCCG 2 cut(s) 353, 479
Eco88I CYCGRG 1 cut(s) 551
EcoO109I RGGNCCY 3 cut(s) 586, 691, 862
EcoRII CCWGG 2 cut(s) 565, 864
EcoT14I CCWWGG 3 cut(s) 594, 723, 750
EcoT38I GRGCYC 1 cut(s) 589
ErhI CCWWGG 3 cut(s) 594, 723, 750
Esp3I CGTCTC 2 cut(s) 291, 509
FaeI CATG 1 cut(s) 499
FaiI YATR 3 cut(s) 165, 292, 497
FaqI GGGAC 1 cut(s) 704
FatI CATG 1 cut(s) 495
FauI CCCGC 3 cut(s) 170, 597, 638
FokI GGATG 2 cut(s) 422, 897
FriOI GRGCYC 1 cut(s) 589
GlaI GCGC 5 cut(s) 213, 303, 512, 803, 817
HaeIII GGCC 6 cut(s) 345, 355, 481, 542, 587, 755
HapII CCGG 4 cut(s) 352, 356, 663, 790
HgaI GACGC 3 cut(s) 308, 461, 510
HhaI GCGC 5 cut(s) 214, 304, 513, 804, 818
Hin1I GRCGYC 2 cut(s) 308, 521
Hin1II CATG 1 cut(s) 499
Hin6I GCGC 5 cut(s) 212, 302, 511, 802, 816
HinP1I GCGC 5 cut(s) 212, 302, 511, 802, 816
HinfI GANTC 3 cut(s) 102, 425, 602
HpaII CCGG 4 cut(s) 352, 356, 663, 790
HphI GGTGA 1 cut(s) 180
Hpy166II GTNNAC 1 cut(s) 361
Hpy188I TCNGA 9 cut(s) 26, 51, 76, 90, 430, 538, 613, 705, 920
Hpy188III TCNNGA 5 cut(s) 122, 496, 553, 689, 790
Hpy8I GTNNAC 1 cut(s) 361
HpyAV CCTTC 3 cut(s) 610, 704, 757
HpyCH4III ACNGT 1 cut(s) 840
HpyCH4IV ACGT 6 cut(s) 159, 228, 308, 405, 777, 885
HpyCH4V TGCA 3 cut(s) 4, 294, 910
HpyF3I CTNAG 3 cut(s) 87, 485, 896
HpySE526I ACGT 6 cut(s) 159, 228, 308, 405, 777, 885
Hsp92I GRCGYC 2 cut(s) 308, 521
Hsp92II CATG 1 cut(s) 499
HspAI GCGC 5 cut(s) 212, 302, 511, 802, 816
Kpn2I TCCGGA 1 cut(s) 789
KspI CCGCGG 3 cut(s) 197, 441, 759
Kzo9I GATC 3 cut(s) 90, 348, 498
LguI GCTCTTC 1 cut(s) 644
LmnI GCTCC 5 cut(s) 124, 202, 508, 705, 725
Lsp1109I GCAGC 4 cut(s) 4, 720, 723, 816
LweI GCATC 1 cut(s) 400
MabI ACCWGGT 1 cut(s) 864
MaeII ACGT 6 cut(s) 159, 228, 308, 405, 777, 885
MaeIII GTNAC 2 cut(s) 460, 773
MalI GATC 3 cut(s) 92, 350, 500
MbiI CCGCTC 2 cut(s) 9, 654
MboI GATC 3 cut(s) 90, 348, 498
MboII GAAGA 7 cut(s) 60, 254, 361, 430, 600, 661, 685
MflI RGATCY 1 cut(s) 348
MhlI GDGCHC 2 cut(s) 530, 589
MluCI AATT 4 cut(s) 182, 627, 730, 904
MluI ACGCGT 1 cut(s) 60
MlyI GAGTC 1 cut(s) 111
MmeI TCCRAC 2 cut(s) 99, 180
MroI TCCGGA 1 cut(s) 789
MseI TTAA 1 cut(s) 35
MspA1I CMGCKG 4 cut(s) 196, 440, 758, 807
MspI CCGG 4 cut(s) 352, 356, 663, 790
MspR9I CCNGG 3 cut(s) 567, 664, 866
Mva1269I GAATGC 1 cut(s) 495
MvaI CCWGG 2 cut(s) 567, 866
MvnI CGCG 9 cut(s) 62, 196, 214, 302, 440, 472, 513, 758, 818
NciI CCSGG 1 cut(s) 664
NdeII GATC 3 cut(s) 90, 348, 498
NlaIII CATG 1 cut(s) 499
NlaIV GGNNCC 4 cut(s) 587, 588, 692, 721
PaeR7I CTCGAG 1 cut(s) 551
PagI TCATGA 1 cut(s) 495
PciSI GCTCTTC 1 cut(s) 644
PcsI WCGNNNNNNNCGW 8 cut(s) 38, 47, 225, 402, 411, 665, 783, 848
PctI GAATGC 1 cut(s) 495
PfeI GAWTC 2 cut(s) 425, 602
PflFI GACNNNGTC 2 cut(s) 520, 664
PleI GAGTC 1 cut(s) 110
PpsI GAGTC 1 cut(s) 110
PpuMI RGGWCCY 2 cut(s) 691, 862
Psp5II RGGWCCY 2 cut(s) 691, 862
Psp6I CCWGG 2 cut(s) 565, 864
PspGI CCWGG 2 cut(s) 565, 864
PspN4I GGNNCC 4 cut(s) 587, 588, 692, 721
PspOMI GGGCCC 1 cut(s) 585
PspPI GGNCC 4 cut(s) 585, 586, 691, 862
PspPPI RGGWCCY 2 cut(s) 691, 862
PstNI CAGNNNCTG 1 cut(s) 865
PsuI RGATCY 1 cut(s) 348
PsyI GACNNNGTC 2 cut(s) 520, 664
RsaI GTAC 1 cut(s) 854
RsaNI GTAC 1 cut(s) 853
SacII CCGCGG 3 cut(s) 197, 441, 759
SapI GCTCTTC 1 cut(s) 644
SaqAI TTAA 1 cut(s) 35
Sau3AI GATC 3 cut(s) 90, 348, 498
Sau96I GGNCC 4 cut(s) 585, 586, 691, 862
SchI GAGTC 1 cut(s) 111
ScrFI CCNGG 3 cut(s) 567, 664, 866
SduI GDGCHC 2 cut(s) 530, 589
SexAI ACCWGGT 1 cut(s) 864
SfaNI GCATC 1 cut(s) 400
Sfr274I CTCGAG 1 cut(s) 551
Sfr303I CCGCGG 3 cut(s) 197, 441, 759
SgrBI CCGCGG 3 cut(s) 197, 441, 759
SinI GGWCC 2 cut(s) 691, 862
SlaI CTCGAG 1 cut(s) 551
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
Sse9I AATT 4 cut(s) 182, 627, 730, 904
StyD4I CCNGG 3 cut(s) 565, 662, 864
StyI CCWWGG 3 cut(s) 594, 723, 750
TaaI ACNGT 1 cut(s) 840
TaiI ACGT 6 cut(s) 162, 231, 311, 408, 780, 888
TaqI TCGA 5 cut(s) 372, 501, 552, 659, 671
TasI AATT 4 cut(s) 182, 627, 730, 904
TfiI GAWTC 2 cut(s) 425, 602
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TseI GCWGC 4 cut(s) 17, 708, 711, 804
Tth111I GACNNNGTC 2 cut(s) 520, 664
VpaK11BI GGWCC 2 cut(s) 691, 862
XapI RAATTY 1 cut(s) 904
XcmI CCANNNNNNNNNTGG 1 cut(s) 730
XhoI CTCGAG 1 cut(s) 551
ZraI GACGTC 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.