AT5G62960

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007001
Physical Location & Seq
Forward (+)
25268950 .. 25271823
2874 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G62960.2

Sequence Viewer

Length: 1044 bp
ATGGTCTCATCGTCTTCATCATTGTTATCTCCATCTTCATATACAGAGTTAAAAGATGCTTGGCATCCATCAACAACAGCGAACACAACAGAATCAAGCTACTGGTTTAATTGGAGGGTTATGATCTGCTGCATATGGATGGCTATAGCGACTGTGATCACAGCTTTTCTTATATTCAAATACGAAGGTTTTCGTCGGAAACGAAGTGACGTTGGTGAAGTTGACGGAGGAGAGAAAGAGTGGTCGGGAAATGTATATGAAGACGAGACATGGAGGCCTTGTCTTCGTAATATTCATCCAGCTTGGCTTCTTGCTTTCCGAGTTGTTGCCTTCTTCGTTCTTCTTGTTATGTTGATTGTTATCGGCCTTGTTGATGGACCTACCATCTTCTTCTACTACACACAGTGGACTTTTGGTCTGATCACTCTCTATTTCGGATTAGGCTCGCTTCTTTCTTTGCACGGATGTTACCAATACAACAAAAGAGCAGCTGGCGATAGAGTAGATAGCATTGAAGCCATAGACTCAGAGAGAGCAAGATCCAAAGGAGCTGATAATACTATTCAACAGAGTCAATACTCTAGTAATCCAGCTGGTTTTTGGGGATATGTTTTCCAGATTATCTTTCAGATGAATGCAGGTGCAGTTTTGCTTACTGACTGTGTCTTCTGGTTCATCATTGTTCCTTTCCTTGAGATCCATGATTATAGCCTCAATGTTCTGGTGATCAACATGCACTCTCTTAATGCGATTTTCTTGCTTGGTGATGCTGCATTGAATTCTCTGAGCTTTCCATGCTTCAGAATTGCTTACTTCTTCTTCTGGACTATAGCTTATGTTATATTCCAATGGGCCCTCCACTCCTTAGTCCATATATGGTGGCCTTACCCATTCTTGGACTTATCATCACACTATGCTCCACTATGGTACTTCTCAGTGGCAGTGATGCATTTGCCATGCTATGGAGCCTTTGCCTTATTGGTGAAGCTAAAACATCGGCTTCTACAGAGATGGTTTCCTGAGTCGTACCAGAGTCCTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

347

Amino Acids

40.14

Weight (kDa)

6.54

Isoelectric Point (pI)

45.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 629
Acc36I ACCTGC 1 cut(s) 629
AccB7I CCANNNNNTGG 1 cut(s) 962
AclWI GGATC 2 cut(s) 534, 691
AcsI RAATTY 1 cut(s) 778
AcuI CTGAAG 1 cut(s) 784
AdeI CACNNNGTG 1 cut(s) 405
AfaI GTAC 2 cut(s) 929, 1028
AfiI CCNNNNNNNGG 2 cut(s) 895, 962
AgsI TTSAA 4 cut(s) 178, 515, 566, 778
AhdI GACNNNNNGTC 1 cut(s) 414
AluBI AGCT 9 cut(s) 99, 164, 302, 491, 551, 593, 789, 833, 988
AluI AGCT 9 cut(s) 99, 164, 302, 491, 551, 593, 789, 833, 988
Alw26I GTCTC 2 cut(s) 10, 260
AlwI GGATC 2 cut(s) 534, 691
AoxI GGCC 4 cut(s) 275, 364, 852, 881
ApaI GGGCCC 1 cut(s) 856
ApeKI GCWGC 3 cut(s) 129, 488, 770
ApoI RAATTY 1 cut(s) 778
Asp700I GAANNNNTTC 1 cut(s) 189
AspS9I GGNCC 3 cut(s) 377, 852, 853
AsuHPI GGTGA 4 cut(s) 227, 736, 776, 994
AvaII GGWCC 1 cut(s) 377
BaeGI GKGCMC 1 cut(s) 856
BanII GRGCYC 1 cut(s) 856
BbsI GAAGAC 4 cut(s) 6, 267, 275, 658
BbvI GCAGC 3 cut(s) 116, 500, 757
BccI CCATC 6 cut(s) 40, 76, 133, 368, 392, 1005
BcgI CGANNNNNNTGC 2 cut(s) 739, 773
BclI TGATCA 3 cut(s) 156, 420, 726
BcoDI GTCTC 2 cut(s) 10, 260
BfaI CTAG 1 cut(s) 582
BfmI CTRYAG 3 cut(s) 144, 828, 1004
BfuAI ACCTGC 1 cut(s) 629
BisI GCNGC 3 cut(s) 130, 489, 771
BlsI GCNGC 3 cut(s) 131, 490, 772
Bme18I GGWCC 1 cut(s) 377
BmeRI GACNNNNNGTC 1 cut(s) 414
BmgT120I GGNCC 3 cut(s) 377, 852, 853
BmiI GGNNCC 2 cut(s) 854, 967
BmsI GCATC 4 cut(s) 46, 73, 757, 936
BpiI GAAGAC 4 cut(s) 6, 267, 275, 658
BpuEI CTTGAG 1 cut(s) 713
BsaBI GATNNNNATC 1 cut(s) 359
BsaI GGTCTC 1 cut(s) 10
BsaJI CCNNGG 1 cut(s) 1037
BsaXI ACNNNNNCTCC 2 cut(s) 265, 295
Bsc4I CCNNNNNNNGG 2 cut(s) 895, 962
Bse1I ACTGG 1 cut(s) 107
Bse8I GATNNNNATC 1 cut(s) 359
BseDI CCNNGG 1 cut(s) 1037
BseGI GGATG 4 cut(s) 64, 144, 295, 470
BseJI GATNNNNATC 1 cut(s) 359
BseLI CCNNNNNNNGG 2 cut(s) 895, 962
BseMII CTCAG 4 cut(s) 540, 776, 948, 1011
BseNI ACTGG 1 cut(s) 107
BseRI GAGGAG 1 cut(s) 243
BseSI GKGCMC 1 cut(s) 856
BseXI GCAGC 3 cut(s) 116, 500, 757
BsgI GTGCAG 1 cut(s) 663
BshFI GGCC 4 cut(s) 277, 366, 854, 883
BslI CCNNNNNNNGG 2 cut(s) 895, 962
BsmAI GTCTC 2 cut(s) 10, 260
BsmI GAATGC 1 cut(s) 640
BsnI GGCC 4 cut(s) 277, 366, 854, 883
Bso31I GGTCTC 1 cut(s) 10
Bsp120I GGGCCC 1 cut(s) 852
Bsp1286I GDGCHC 1 cut(s) 856
Bsp143I GATC 6 cut(s) 123, 156, 420, 539, 696, 726
BspANI GGCC 4 cut(s) 277, 366, 854, 883
BspCNI CTCAG 4 cut(s) 539, 777, 947, 1012
BspLI GGNNCC 2 cut(s) 854, 967
BspMI ACCTGC 1 cut(s) 629
BspPI GGATC 2 cut(s) 534, 691
BspTNI GGTCTC 1 cut(s) 10
BsrI ACTGG 1 cut(s) 107
BssECI CCNNGG 1 cut(s) 1037
BssMI GATC 6 cut(s) 123, 156, 420, 539, 696, 726
Bst4CI ACNGT 3 cut(s) 154, 405, 662
BstC8I GCNNGC 2 cut(s) 446, 493
BstDEI CTNAG 5 cut(s) 526, 785, 865, 934, 1020
BstF5I GGATG 4 cut(s) 64, 144, 295, 470
BstKTI GATC 6 cut(s) 126, 159, 423, 542, 699, 729
BstMAI GTCTC 2 cut(s) 10, 260
BstMBI GATC 6 cut(s) 123, 156, 420, 539, 696, 726
BstMWI GCNNNNNNNGC 1 cut(s) 795
BstNSI RCATGY 1 cut(s) 736
BstSFI CTRYAG 3 cut(s) 144, 828, 1004
BstSLI GKGCMC 1 cut(s) 856
BstV1I GCAGC 3 cut(s) 116, 500, 757
BstV2I GAAGAC 4 cut(s) 6, 267, 275, 658
BstX2I RGATCY 2 cut(s) 539, 696
BstYI RGATCY 2 cut(s) 539, 696
BsuRI GGCC 4 cut(s) 277, 366, 854, 883
BtsCI GGATG 4 cut(s) 64, 144, 295, 470
BtsI GCAGTG 1 cut(s) 948
BtsIMutI CAGTG 3 cut(s) 410, 942, 948
BveI ACCTGC 1 cut(s) 629
Cac8I GCNNGC 2 cut(s) 446, 493
Cfr13I GGNCC 3 cut(s) 377, 852, 853
Csp6I GTAC 2 cut(s) 928, 1027
CviAII CATG 5 cut(s) 270, 701, 733, 795, 957
CviQI GTAC 2 cut(s) 928, 1027
DdeI CTNAG 5 cut(s) 526, 785, 865, 934, 1020
DpnI GATC 6 cut(s) 125, 158, 422, 541, 698, 728
DpnII GATC 6 cut(s) 123, 156, 420, 539, 696, 726
DraIII CACNNNGTG 1 cut(s) 405
DriI GACNNNNNGTC 1 cut(s) 414
Eam1105I GACNNNNNGTC 1 cut(s) 414
Eco147I AGGCCT 1 cut(s) 277
Eco24I GRGCYC 1 cut(s) 856
Eco31I GGTCTC 1 cut(s) 10
Eco47I GGWCC 1 cut(s) 377
Eco57I CTGAAG 1 cut(s) 784
EcoO109I RGGNCCY 1 cut(s) 853
EcoRI GAATTC 1 cut(s) 778
EcoT22I ATGCAT 1 cut(s) 951
EcoT38I GRGCYC 1 cut(s) 856
FaeI CATG 5 cut(s) 273, 704, 736, 798, 960
FatI CATG 5 cut(s) 269, 700, 732, 794, 956
FauNDI CATATG 1 cut(s) 134
FbaI TGATCA 3 cut(s) 156, 420, 726
Fnu4HI GCNGC 3 cut(s) 130, 489, 771
FokI GGATG 4 cut(s) 51, 151, 282, 477
FriOI GRGCYC 1 cut(s) 856
Fsp4HI GCNGC 3 cut(s) 130, 489, 771
FspBI CTAG 1 cut(s) 582
GluI GCNGC 3 cut(s) 130, 489, 771
HaeIII GGCC 4 cut(s) 277, 366, 854, 883
Hin1II CATG 5 cut(s) 273, 704, 736, 798, 960
HincII GTYRAC 1 cut(s) 223
HindII GTYRAC 1 cut(s) 223
HinfI GANTC 5 cut(s) 92, 524, 571, 1022, 1033
HphI GGTGA 4 cut(s) 227, 736, 776, 994
Hpy166II GTNNAC 2 cut(s) 223, 408
Hpy188I TCNGA 8 cut(s) 198, 320, 420, 437, 529, 630, 786, 803
Hpy188III TCNNGA 4 cut(s) 246, 616, 823, 1019
Hpy8I GTNNAC 2 cut(s) 223, 408
Hpy99I CGWCG 1 cut(s) 198
HpyAV CCTTC 2 cut(s) 179, 340
HpyCH4III ACNGT 3 cut(s) 154, 405, 662
HpyCH4IV ACGT 1 cut(s) 210
HpyCH4V TGCA 7 cut(s) 132, 460, 638, 644, 736, 773, 949
HpyF10VI GCNNNNNNNGC 1 cut(s) 795
HpyF3I CTNAG 5 cut(s) 526, 785, 865, 934, 1020
HpySE526I ACGT 1 cut(s) 210
Hsp92II CATG 5 cut(s) 273, 704, 736, 798, 960
Ksp22I TGATCA 3 cut(s) 156, 420, 726
Kzo9I GATC 6 cut(s) 123, 156, 420, 539, 696, 726
LmnI GCTCC 3 cut(s) 548, 922, 965
Lsp1109I GCAGC 3 cut(s) 116, 500, 757
LweI GCATC 4 cut(s) 46, 73, 757, 936
MaeI CTAG 1 cut(s) 582
MaeII ACGT 1 cut(s) 210
MaeIII GTNAC 2 cut(s) 206, 467
MalI GATC 6 cut(s) 125, 158, 422, 541, 698, 728
MboI GATC 6 cut(s) 123, 156, 420, 539, 696, 726
MflI RGATCY 2 cut(s) 539, 696
MhlI GDGCHC 1 cut(s) 856
MluCI AATT 3 cut(s) 109, 778, 804
MlyI GAGTC 4 cut(s) 518, 580, 1031, 1042
MmeI TCCRAC 1 cut(s) 176
MnlI CCTC 5 cut(s) 108, 221, 267, 722, 866
Mph1103I ATGCAT 1 cut(s) 951
MroXI GAANNNNTTC 1 cut(s) 189
MseI TTAA 3 cut(s) 50, 108, 744
MslI CAYNNNNRTG 1 cut(s) 137
MspA1I CMGCKG 2 cut(s) 491, 593
Mva1269I GAATGC 1 cut(s) 640
MwoI GCNNNNNNNGC 1 cut(s) 795
NdeI CATATG 1 cut(s) 134
NdeII GATC 6 cut(s) 123, 156, 420, 539, 696, 726
NlaIII CATG 5 cut(s) 273, 704, 736, 798, 960
NlaIV GGNNCC 2 cut(s) 854, 967
NmuCI GTSAC 1 cut(s) 206
NsiI ATGCAT 1 cut(s) 951
NspI RCATGY 1 cut(s) 736
PaqCI CACCTGC 1 cut(s) 629
PceI AGGCCT 1 cut(s) 277
PcsI WCGNNNNNNNCGW 1 cut(s) 199
PctI GAATGC 1 cut(s) 640
PdmI GAANNNNTTC 1 cut(s) 189
PfeI GAWTC 1 cut(s) 92
PflFI GACNNNGTC 1 cut(s) 662
PflMI CCANNNNNTGG 1 cut(s) 962
PkrI GCNGC 3 cut(s) 131, 490, 772
PleI GAGTC 4 cut(s) 518, 579, 1030, 1041
PpsI GAGTC 4 cut(s) 518, 579, 1030, 1041
PspN4I GGNNCC 2 cut(s) 854, 967
PspOMI GGGCCC 1 cut(s) 852
PspPI GGNCC 3 cut(s) 377, 852, 853
PsuI RGATCY 2 cut(s) 539, 696
PsyI GACNNNGTC 1 cut(s) 662
PvuII CAGCTG 2 cut(s) 491, 593
RsaI GTAC 2 cut(s) 929, 1028
RsaNI GTAC 2 cut(s) 928, 1027
RseI CAYNNNNRTG 1 cut(s) 137
SaqAI TTAA 3 cut(s) 50, 108, 744
SatI GCNGC 3 cut(s) 130, 489, 771
Sau3AI GATC 6 cut(s) 123, 156, 420, 539, 696, 726
Sau96I GGNCC 3 cut(s) 377, 852, 853
SchI GAGTC 4 cut(s) 518, 580, 1031, 1042
SduI GDGCHC 1 cut(s) 856
SfaNI GCATC 4 cut(s) 46, 73, 757, 936
SfcI CTRYAG 3 cut(s) 144, 828, 1004
SinI GGWCC 1 cut(s) 377
SmiMI CAYNNNNRTG 1 cut(s) 137
SmlI CTYRAG 1 cut(s) 692
SmoI CTYRAG 1 cut(s) 692
Sse9I AATT 3 cut(s) 109, 778, 804
SseBI AGGCCT 1 cut(s) 277
SspI AATATT 1 cut(s) 292
SspMI CTAG 1 cut(s) 582
StuI AGGCCT 1 cut(s) 277
TaaI ACNGT 3 cut(s) 154, 405, 662
TaiI ACGT 1 cut(s) 213
TasI AATT 3 cut(s) 109, 778, 804
TfiI GAWTC 1 cut(s) 92
Tru1I TTAA 3 cut(s) 50, 108, 744
Tru9I TTAA 3 cut(s) 50, 108, 744
TscAI CASTG 3 cut(s) 410, 942, 948
TseFI GTSAC 1 cut(s) 206
TseI GCWGC 3 cut(s) 129, 488, 770
Tsp45I GTSAC 1 cut(s) 206
TspDTI ATGAA 6 cut(s) 6, 27, 273, 284, 647, 664
TspGWI ACGGA 2 cut(s) 240, 477
TspRI CASTG 3 cut(s) 410, 942, 948
Tth111I GACNNNGTC 1 cut(s) 662
Van91I CCANNNNNTGG 1 cut(s) 962
VpaK11BI GGWCC 1 cut(s) 377
XapI RAATTY 1 cut(s) 778
XceI RCATGY 1 cut(s) 736
XcmI CCANNNNNNNNNTGG 1 cut(s) 597
XmnI GAANNNNTTC 1 cut(s) 189
XspI CTAG 1 cut(s) 582
Zsp2I ATGCAT 1 cut(s) 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.