FvH4_5g05450

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007001
Physical Location & Seq
Forward (+)
3198709 .. 3204083
5375 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g05450.t1

Sequence Viewer

Length: 1023 bp
ATGAAGAGCAATATAATGGGAACAAAAAGTTCTTGGCAGCCAATCATGACTGCAGATACAACAGCCTCGAGTTACTGGCTTAACTGGAGGGTTTTGCTTTGTTCTATATGGGTCATGATCTCCCTGGCTCTTTCAGTACTTCTCATATGGAGATTTGAAGGGCTTCGAAACTCGAGGCGTAGTAATAACAGCAGAGTAACTCAGCAAGAAGAAGAAGAAACAATTGGAACTTTGTTTGAGGATGAAACTTGGAGACCATGCCTCAAAGGAATCCACCCAGCTTGGCTTCTGGCTTTTAGACTTGTTGCTTTTGTTGTGCTTTTGGTTCTGCTCATCATCACAGCTTGTGTTGATGGAGGTACCATATTTTATTTCTATACACAGTGGACGTTCACATTGGTTACTGTTTACTTTGGGCTTGGATCATTGCTCTCCATTCGAGGATGCTACCGATACCATAAGAAAACTGCTGGTGAAAGGGTTGGTAATGTAGAGCTAGATACAGAACAGGGCACCGTAGAGAAATCCATAGTCTCTCATGTTGCACAACAGAATCAACCACGCCATCCTGCAGGCATTTTGGGTTATGTTTTCCAAATAATTTTCCAGATGAATGCAGGAGCTGTCATCCTCACAGACTGCGTCTTCTGGTTCATAATCGTTCCATTTCTTACAATCAAAGATTACAACTTGAACTTTCTGATTATAAACATGCACACAATCAATGCTGTTTTCTTGCTTGCTGAAACTGCTTTGAATAGCTTGCCATTTCCTTGGTTCAGAATAGGATATTTCTACCTGTGGACAATTACCTATGTAATATTTCAGTGGATATGTCATGCTTGTGTCAGAATTTGGTGGCCATATCCATTTCTCGACTTGTCATCTCCATATGCTCCATTGTGGTATTTGTCGGTCGGTCTACTGCATTTCCCATGCTACGGCATTTTTGCTCTGATAATGAAGCTGAAACACCACTTGTTTTCGACAAGGTGCCCTGACTCTTATCAATGCGGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

341

Amino Acids

39.41

Weight (kDa)

8.63

Isoelectric Point (pI)

34.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 707
Acc65I GGTACC 1 cut(s) 359
AccB1I GGYRCC 3 cut(s) 359, 512, 993
AccI GTMKAC 1 cut(s) 922
AciI CCGC 1 cut(s) 1014
AclWI GGATC 1 cut(s) 430
AcoI YGGCCR 1 cut(s) 860
AcsI RAATTY 1 cut(s) 852
AfaI GTAC 2 cut(s) 138, 361
AfiI CCNNNNNNNGG 1 cut(s) 941
AgsI TTSAA 3 cut(s) 158, 694, 757
AjnI CCWGG 1 cut(s) 123
AjuI GAANNNNNNNTTGG 2 cut(s) 207, 239
AluBI AGCT 6 cut(s) 281, 344, 496, 623, 762, 967
AluI AGCT 6 cut(s) 281, 344, 496, 623, 762, 967
Alw26I GTCTC 2 cut(s) 247, 538
AlwI GGATC 1 cut(s) 430
AlwNI CAGNNNCTG 1 cut(s) 623
Ama87I CYCGRG 2 cut(s) 67, 172
AoxI GGCC 1 cut(s) 860
ApeKI GCWGC 1 cut(s) 37
ApoI RAATTY 1 cut(s) 852
Asp700I GAANNNNTTC 1 cut(s) 162
Asp718I GGTACC 1 cut(s) 359
AsuHPI GGTGA 1 cut(s) 485
AsuII TTCGAA 1 cut(s) 166
AvaI CYCGRG 2 cut(s) 67, 172
BaeGI GKGCMC 2 cut(s) 515, 998
BalI TGGCCA 1 cut(s) 862
BanI GGYRCC 3 cut(s) 359, 512, 993
BbsI GAAGAC 1 cut(s) 637
BbvI GCAGC 1 cut(s) 49
BccI CCATC 2 cut(s) 347, 573
BceAI ACGGC 1 cut(s) 958
BciT130I CCWGG 1 cut(s) 125
BcoDI GTCTC 2 cut(s) 247, 538
BfaI CTAG 1 cut(s) 497
BfmI CTRYAG 2 cut(s) 51, 570
BisI GCNGC 1 cut(s) 38
BlsI GCNGC 1 cut(s) 39
BmcAI AGTACT 1 cut(s) 138
Bme1390I CCNGG 1 cut(s) 125
BmeT110I CYCGRG 2 cut(s) 67, 172
BmiI GGNNCC 3 cut(s) 361, 514, 995
BmrFI CCNGG 1 cut(s) 125
BmsI GCATC 1 cut(s) 434
BpiI GAAGAC 1 cut(s) 637
BpmI CTGGAG 1 cut(s) 106
Bpu14I TTCGAA 1 cut(s) 166
BsaI GGTCTC 1 cut(s) 247
BsaJI CCNNGG 2 cut(s) 123, 773
Bsc4I CCNNNNNNNGG 1 cut(s) 941
Bse1I ACTGG 2 cut(s) 80, 89
Bse3DI GCAATG 1 cut(s) 425
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 2 cut(s) 123, 773
BseGI GGATG 4 cut(s) 247, 449, 565, 627
BseLI CCNNNNNNNGG 1 cut(s) 941
BseMI GCAATG 1 cut(s) 425
BseMII CTCAG 1 cut(s) 215
BseNI ACTGG 2 cut(s) 80, 89
BseSI GKGCMC 2 cut(s) 515, 998
BseXI GCAGC 1 cut(s) 49
BseYI CCCAGC 1 cut(s) 277
Bsh1285I CGRYCG 1 cut(s) 918
BshFI GGCC 1 cut(s) 862
BshNI GGYRCC 3 cut(s) 359, 512, 993
BsiEI CGRYCG 1 cut(s) 918
BsiHKCI CYCGRG 2 cut(s) 67, 172
BslI CCNNNNNNNGG 1 cut(s) 941
BsmAI GTCTC 2 cut(s) 247, 538
BsmI GAATGC 1 cut(s) 619
BsnI GGCC 1 cut(s) 862
Bso31I GGTCTC 1 cut(s) 247
BsoBI CYCGRG 2 cut(s) 67, 172
Bsp119I TTCGAA 1 cut(s) 166
Bsp1286I GDGCHC 2 cut(s) 515, 998
Bsp143I GATC 2 cut(s) 117, 422
BspACI CCGC 1 cut(s) 1014
BspANI GGCC 1 cut(s) 862
BspCNI CTCAG 1 cut(s) 214
BspHI TCATGA 2 cut(s) 45, 114
BspLI GGNNCC 3 cut(s) 361, 514, 995
BspMAI CTGCAG 2 cut(s) 55, 574
BspPI GGATC 1 cut(s) 430
BspT104I TTCGAA 1 cut(s) 166
BspT107I GGYRCC 3 cut(s) 359, 512, 993
BspTNI GGTCTC 1 cut(s) 247
BsrDI GCAATG 1 cut(s) 425
BsrI ACTGG 2 cut(s) 80, 89
BssECI CCNNGG 2 cut(s) 123, 773
BssMI GATC 2 cut(s) 117, 422
BssT1I CCWWGG 1 cut(s) 773
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 3 cut(s) 384, 406, 517
BstBI TTCGAA 1 cut(s) 166
BstC8I GCNNGC 3 cut(s) 574, 741, 764
BstDEI CTNAG 1 cut(s) 201
BstF5I GGATG 4 cut(s) 247, 449, 565, 627
BstKTI GATC 2 cut(s) 120, 425
BstMAI GTCTC 2 cut(s) 247, 538
BstMBI GATC 2 cut(s) 117, 422
BstMCI CGRYCG 1 cut(s) 918
BstMWI GCNNNNNNNGC 1 cut(s) 749
BstNI CCWGG 1 cut(s) 125
BstNSI RCATGY 1 cut(s) 715
BstSCI CCNGG 1 cut(s) 123
BstSFI CTRYAG 2 cut(s) 51, 570
BstSLI GKGCMC 2 cut(s) 515, 998
BstV1I GCAGC 1 cut(s) 49
BstV2I GAAGAC 1 cut(s) 637
BstXI CCANNNNNNTGG 1 cut(s) 774
BsuRI GGCC 1 cut(s) 862
BtsCI GGATG 4 cut(s) 247, 449, 565, 627
BtsIMutI CAGTG 2 cut(s) 389, 833
Cac8I GCNNGC 3 cut(s) 574, 741, 764
CaiI CAGNNNCTG 1 cut(s) 623
CciI TCATGA 2 cut(s) 45, 114
CseI GACGC 1 cut(s) 631
Csp6I GTAC 2 cut(s) 137, 360
CspCI CAANNNNNGTGG 2 cut(s) 263, 298
CviAII CATG 7 cut(s) 46, 115, 258, 539, 712, 839, 936
CviQI GTAC 2 cut(s) 137, 360
DdeI CTNAG 1 cut(s) 201
DpnI GATC 2 cut(s) 119, 424
DpnII GATC 2 cut(s) 117, 422
EaeI YGGCCR 1 cut(s) 860
Eco130I CCWWGG 1 cut(s) 773
Eco31I GGTCTC 1 cut(s) 247
Eco88I CYCGRG 2 cut(s) 67, 172
EcoRII CCWGG 1 cut(s) 123
EcoT14I CCWWGG 1 cut(s) 773
ErhI CCWWGG 1 cut(s) 773
FaeI CATG 7 cut(s) 49, 118, 261, 542, 715, 842, 939
FatI CATG 7 cut(s) 45, 114, 257, 538, 711, 838, 935
FauI CCCGC 1 cut(s) 1007
FauNDI CATATG 2 cut(s) 146, 892
FblI GTMKAC 1 cut(s) 922
Fnu4HI GCNGC 1 cut(s) 38
FokI GGATG 4 cut(s) 254, 456, 552, 614
Fsp4HI GCNGC 1 cut(s) 38
FspBI CTAG 1 cut(s) 497
GluI GCNGC 1 cut(s) 38
GsaI CCCAGC 1 cut(s) 281
GsuI CTGGAG 1 cut(s) 106
HaeIII GGCC 1 cut(s) 862
HgaI GACGC 1 cut(s) 631
Hin1II CATG 7 cut(s) 49, 118, 261, 542, 715, 842, 939
HinfI GANTC 3 cut(s) 270, 553, 1001
HphI GGTGA 1 cut(s) 485
Hpy166II GTNNAC 5 cut(s) 387, 393, 409, 804, 923
Hpy188I TCNGA 4 cut(s) 702, 782, 851, 957
Hpy188III TCNNGA 4 cut(s) 46, 115, 607, 875
Hpy8I GTNNAC 5 cut(s) 387, 393, 409, 804, 923
HpyAV CCTTC 1 cut(s) 152
HpyCH4III ACNGT 3 cut(s) 384, 406, 517
HpyCH4IV ACGT 1 cut(s) 389
HpyCH4V TGCA 6 cut(s) 53, 545, 572, 617, 715, 928
HpyF10VI GCNNNNNNNGC 1 cut(s) 749
HpyF3I CTNAG 1 cut(s) 201
HpySE526I ACGT 1 cut(s) 389
Hsp92II CATG 7 cut(s) 49, 118, 261, 542, 715, 842, 939
KpnI GGTACC 1 cut(s) 363
Kzo9I GATC 2 cut(s) 117, 422
LmnI GCTCC 2 cut(s) 620, 901
Lsp1109I GCAGC 1 cut(s) 49
LweI GCATC 1 cut(s) 434
MaeI CTAG 1 cut(s) 497
MaeII ACGT 1 cut(s) 389
MaeIII GTNAC 3 cut(s) 71, 196, 400
MalI GATC 2 cut(s) 119, 424
MboI GATC 2 cut(s) 117, 422
MboII GAAGA 5 cut(s) 16, 221, 224, 227, 637
MfeI CAATTG 1 cut(s) 222
MhlI GDGCHC 2 cut(s) 515, 998
MlsI TGGCCA 1 cut(s) 862
MluCI AATT 4 cut(s) 222, 600, 807, 852
MluNI TGGCCA 1 cut(s) 862
MlyI GAGTC 1 cut(s) 995
MnlI CCTC 9 cut(s) 76, 81, 168, 232, 272, 350, 434, 641, 1011
Mox20I TGGCCA 1 cut(s) 862
MroXI GAANNNNTTC 1 cut(s) 162
MscI TGGCCA 1 cut(s) 862
MseI TTAA 1 cut(s) 81
MslI CAYNNNNRTG 1 cut(s) 843
Msp20I TGGCCA 1 cut(s) 862
MspR9I CCNGG 1 cut(s) 125
MunI CAATTG 1 cut(s) 222
Mva1269I GAATGC 1 cut(s) 619
MvaI CCWGG 1 cut(s) 125
MwoI GCNNNNNNNGC 1 cut(s) 749
NdeI CATATG 2 cut(s) 146, 892
NdeII GATC 2 cut(s) 117, 422
NlaIII CATG 7 cut(s) 49, 118, 261, 542, 715, 842, 939
NlaIV GGNNCC 3 cut(s) 361, 514, 995
NspI RCATGY 1 cut(s) 715
NspV TTCGAA 1 cut(s) 166
PaeR7I CTCGAG 2 cut(s) 67, 172
PagI TCATGA 2 cut(s) 45, 114
PctI GAATGC 1 cut(s) 619
PdmI GAANNNNTTC 1 cut(s) 162
PfeI GAWTC 2 cut(s) 270, 553
PflFI GACNNNGTC 1 cut(s) 641
PkrI GCNGC 1 cut(s) 39
PleI GAGTC 1 cut(s) 995
PpsI GAGTC 1 cut(s) 995
PsiI TTATAA 1 cut(s) 707
Psp6I CCWGG 1 cut(s) 123
PspFI CCCAGC 1 cut(s) 277
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 3 cut(s) 361, 514, 995
PspXI VCTCGAGB 2 cut(s) 67, 172
PstI CTGCAG 2 cut(s) 55, 574
PstNI CAGNNNCTG 1 cut(s) 623
PsyI GACNNNGTC 1 cut(s) 641
RsaI GTAC 2 cut(s) 138, 361
RsaNI GTAC 2 cut(s) 137, 360
RseI CAYNNNNRTG 1 cut(s) 843
SaqAI TTAA 1 cut(s) 81
SatI GCNGC 1 cut(s) 38
Sau3AI GATC 2 cut(s) 117, 422
SbfI CCTGCAGG 1 cut(s) 574
ScaI AGTACT 1 cut(s) 138
SchI GAGTC 1 cut(s) 995
ScrFI CCNGG 1 cut(s) 125
SdaI CCTGCAGG 1 cut(s) 574
SduI GDGCHC 2 cut(s) 515, 998
SfaNI GCATC 1 cut(s) 434
SfcI CTRYAG 2 cut(s) 51, 570
Sfr274I CTCGAG 2 cut(s) 67, 172
SfuI TTCGAA 1 cut(s) 166
SlaI CTCGAG 2 cut(s) 67, 172
SmiMI CAYNNNNRTG 1 cut(s) 843
SmlI CTYRAG 2 cut(s) 67, 172
SmoI CTYRAG 2 cut(s) 67, 172
Sse8387I CCTGCAGG 1 cut(s) 574
Sse9I AATT 4 cut(s) 222, 600, 807, 852
SsiI CCGC 1 cut(s) 1014
SspI AATATT 1 cut(s) 822
SspMI CTAG 1 cut(s) 497
StyD4I CCNGG 1 cut(s) 123
StyI CCWWGG 1 cut(s) 773
TaaI ACNGT 3 cut(s) 384, 406, 517
TaiI ACGT 1 cut(s) 392
TaqI TCGA 6 cut(s) 68, 166, 173, 439, 876, 986
TaqII GACCGA 2 cut(s) 904, 908
TasI AATT 4 cut(s) 222, 600, 807, 852
TatI WGTACW 1 cut(s) 136
TfiI GAWTC 2 cut(s) 270, 553
Tru1I TTAA 1 cut(s) 81
Tru9I TTAA 1 cut(s) 81
TscAI CASTG 2 cut(s) 389, 833
TseI GCWGC 1 cut(s) 37
TspDTI ATGAA 5 cut(s) 17, 258, 626, 643, 977
TspRI CASTG 2 cut(s) 389, 833
Tth111I GACNNNGTC 1 cut(s) 641
XapI RAATTY 1 cut(s) 852
XceI RCATGY 1 cut(s) 715
XhoI CTCGAG 2 cut(s) 67, 172
XmiI GTMKAC 1 cut(s) 922
XmnI GAANNNNTTC 1 cut(s) 162
XspI CTAG 1 cut(s) 497
ZrmI AGTACT 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.