MD06G1172600.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007001
Physical Location & Seq
Reverse (-)
31269406 .. 31271517
2112 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1172600.v1.1.491

Sequence Viewer

Length: 1062 bp
ATGGGTTTTATAACAGGAGTTCCAAAAGGTTCTTCTTGGCAGCCAATCATGACTGCGGACACAACAACTTCAAGTTACTGGATCAACTGGAGGGTCTTGCTCTGCACGATATGGATATCGATTGCGATGACTCTTTCGGTGATTCTGGTTTGCAAATACGAAGAAAGACCGCGCAGTTCAAAGCGCGGTAACAGCGGAGAAAACCAGCAACAAACGTCTGCAGGAACATTGTTTGAGGATGAGACTTGGAGACCATGCCTCAAGGGAATTCACCCGGCTTGGCTCCTGGCTTTTCGGGTTTTCGCTTTCATTGTGCTCTTGGTTCTGCTCATCATTACCGCTTCCGTGGATGGAGGCAGCATTTTTCTCTACTACACACAGTGGACTTTTACTTTGATCACAATTTATTTTGGGCTTGGATCGTTGCTCTCCATTCGGGGATGCTACCGCTACCATAAGAAAACTGCCGGCGATAGGGTTGATATTTTTGAGGTGGATACAGAGCAAGGCTCTGCTCATGTGACTCCTTCACATGGAGGTTCTTCCAAAGATTCAATCATAGAGAAACCCTCAGGCCCCCATGTCGAACAACCTCAAGTTCGCCAACTTGCTGGCTTTTCGGGTTATATCTTCCAAATAATTTTCCAGATGAATGCCGGCGCTGTCCTTCTCACGGATTGTGTCTTCTGGTTCGTACTTGTTCCTTTTCTTACAATCAAAGACTACAACTTGAATTTTCTGATCATAAATATGCACACGATCAACGCAGTTTTCTTGCTCGCTGATACCGCGTTGAATAGCTTGCCATTTCCCTGGTTCCGAATCGGATATTTCTTCCTGTGGACGATCGTTTATGTAGTTTTCCAGTGGCTCTTCCATGCTTGTGTCAAGCTGTGGTGGCCGTATCCATTTCTAGACTTGTCATCTCCATATGCTCCGCTGTGGTACTTGTCGGTCGGTCTACTGCACATCCCATGCTACAGCATTTTTGTTCTGGTAATGAAGCTCAAATACCATCTGTTTTCGACGCGATACCCTGAATCGTATCAATGCGCGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

354

Amino Acids

40.49

Weight (kDa)

8.54

Isoelectric Point (pI)

30.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 11
AccI GTMKAC 1 cut(s) 961
AccII CGCG 5 cut(s) 172, 186, 791, 1030, 1055
AciI CCGC 8 cut(s) 56, 170, 186, 195, 339, 448, 789, 938
AclWI GGATC 2 cut(s) 89, 427
AcoI YGGCCR 1 cut(s) 899
AcsI RAATTY 2 cut(s) 267, 733
AdeI CACNNNGTG 1 cut(s) 381
AfaI GTAC 2 cut(s) 696, 947
AfiI CCNNNNNNNGG 3 cut(s) 474, 533, 673
AgsI TTSAA 5 cut(s) 72, 180, 555, 733, 796
AjnI CCWGG 2 cut(s) 285, 812
AjuI GAANNNNNNNTTGG 2 cut(s) 16, 48
AluBI AGCT 3 cut(s) 801, 892, 1006
AluI AGCT 3 cut(s) 801, 892, 1006
Alw21I GWGCWC 1 cut(s) 318
Alw26I GTCTC 2 cut(s) 236, 244
AlwI GGATC 2 cut(s) 89, 427
AoxI GGCC 2 cut(s) 574, 899
ApeKI GCWGC 2 cut(s) 40, 357
ApoI RAATTY 2 cut(s) 267, 733
ArsI GACNNNNNNTTYG 2 cut(s) 376, 408
AspLEI GCGC 4 cut(s) 174, 186, 662, 1055
AspS9I GGNCC 1 cut(s) 575
AsuC2I CCSGG 1 cut(s) 275
AsuHPI GGTGA 2 cut(s) 151, 263
AxyI CCTNAGG 1 cut(s) 571
BarI GAAGNNNNNNTAC 2 cut(s) 995, 1027
BbsI GAAGAC 1 cut(s) 676
Bbv12I GWGCWC 1 cut(s) 318
BbvI GCAGC 2 cut(s) 52, 369
BccI CCATC 2 cut(s) 344, 1023
BceAI ACGGC 1 cut(s) 886
BciT130I CCWGG 2 cut(s) 287, 814
BciVI GTATCC 2 cut(s) 490, 915
BclI TGATCA 2 cut(s) 396, 741
BcnI CCSGG 1 cut(s) 275
BcoDI GTCTC 2 cut(s) 236, 244
BfaI CTAG 1 cut(s) 914
BfmI CTRYAG 2 cut(s) 219, 979
BfoI RGCGCY 1 cut(s) 663
BfuI GTATCC 2 cut(s) 490, 915
BisI GCNGC 2 cut(s) 41, 358
BlsI GCNGC 2 cut(s) 42, 359
Bme1390I CCNGG 3 cut(s) 275, 287, 814
BmgT120I GGNCC 1 cut(s) 575
BmiI GGNNCC 3 cut(s) 284, 577, 818
BmrFI CCNGG 3 cut(s) 275, 287, 814
BmsI GCATC 1 cut(s) 431
BpiI GAAGAC 1 cut(s) 676
BplI GAGNNNNNCTC 4 cut(s) 494, 526, 554, 586
BpmI CTGGAG 1 cut(s) 109
BpuEI CTTGAG 2 cut(s) 245, 579
BpuMI CCSGG 1 cut(s) 275
Bsa29I ATCGAT 1 cut(s) 119
BsaI GGTCTC 1 cut(s) 244
BsaJI CCNNGG 2 cut(s) 345, 812
Bsc4I CCNNNNNNNGG 3 cut(s) 474, 533, 673
Bse118I RCCGGY 2 cut(s) 467, 656
Bse1I ACTGG 3 cut(s) 83, 92, 865
Bse21I CCTNAGG 1 cut(s) 571
BseBI CCWGG 2 cut(s) 287, 814
BseCI ATCGAT 1 cut(s) 119
BseDI CCNNGG 2 cut(s) 345, 812
BseGI GGATG 4 cut(s) 244, 355, 446, 969
BseLI CCNNNNNNNGG 3 cut(s) 474, 533, 673
BseMII CTCAG 1 cut(s) 585
BseNI ACTGG 3 cut(s) 83, 92, 865
BseXI GCAGC 2 cut(s) 52, 369
BsgI GTGCAG 2 cut(s) 88, 950
Bsh1236I CGCG 5 cut(s) 172, 186, 791, 1030, 1055
Bsh1285I CGRYCG 2 cut(s) 849, 957
BshFI GGCC 2 cut(s) 576, 901
BshVI ATCGAT 1 cut(s) 119
BsiEI CGRYCG 2 cut(s) 849, 957
BsiHKAI GWGCWC 1 cut(s) 318
BsiSI CCGG 3 cut(s) 275, 468, 657
BslI CCNNNNNNNGG 3 cut(s) 474, 533, 673
BsmAI GTCTC 2 cut(s) 236, 244
BsmI GAATGC 1 cut(s) 658
BsnI GGCC 2 cut(s) 576, 901
Bso31I GGTCTC 1 cut(s) 244
Bsp1286I GDGCHC 1 cut(s) 318
Bsp143I GATC 6 cut(s) 81, 396, 419, 741, 759, 846
BspACI CCGC 8 cut(s) 56, 170, 186, 195, 339, 448, 789, 938
BspANI GGCC 2 cut(s) 576, 901
BspCNI CTCAG 1 cut(s) 584
BspDI ATCGAT 1 cut(s) 119
BspFNI CGCG 5 cut(s) 172, 186, 791, 1030, 1055
BspHI TCATGA 1 cut(s) 48
BspLI GGNNCC 3 cut(s) 284, 577, 818
BspMAI CTGCAG 1 cut(s) 223
BspPI GGATC 2 cut(s) 89, 427
BspQI GCTCTTC 1 cut(s) 878
BspTNI GGTCTC 1 cut(s) 244
BsrFI RCCGGY 2 cut(s) 467, 656
BsrI ACTGG 3 cut(s) 83, 92, 865
BssAI RCCGGY 2 cut(s) 467, 656
BssECI CCNNGG 2 cut(s) 345, 812
BssMI GATC 6 cut(s) 81, 396, 419, 741, 759, 846
Bst2UI CCWGG 2 cut(s) 287, 814
Bst4CI ACNGT 1 cut(s) 381
Bst6I CTCTTC 1 cut(s) 878
BstC8I GCNNGC 5 cut(s) 469, 613, 658, 780, 803
BstDEI CTNAG 1 cut(s) 571
BstDSI CCRYGG 1 cut(s) 345
BstF5I GGATG 4 cut(s) 244, 355, 446, 969
BstFNI CGCG 5 cut(s) 172, 186, 791, 1030, 1055
BstH2I RGCGCY 1 cut(s) 663
BstHHI GCGC 4 cut(s) 174, 186, 662, 1055
BstKTI GATC 6 cut(s) 84, 399, 422, 744, 762, 849
BstMAI GTCTC 2 cut(s) 236, 244
BstMBI GATC 6 cut(s) 81, 396, 419, 741, 759, 846
BstMCI CGRYCG 2 cut(s) 849, 957
BstMWI GCNNNNNNNGC 3 cut(s) 192, 788, 898
BstNI CCWGG 2 cut(s) 287, 814
BstSCI CCNGG 3 cut(s) 273, 285, 812
BstSFI CTRYAG 2 cut(s) 219, 979
BstUI CGCG 5 cut(s) 172, 186, 791, 1030, 1055
BstV1I GCAGC 2 cut(s) 52, 369
BstV2I GAAGAC 1 cut(s) 676
BstXI CCANNNNNNTGG 2 cut(s) 611, 813
Bsu15I ATCGAT 1 cut(s) 119
Bsu36I CCTNAGG 1 cut(s) 571
BsuI GTATCC 2 cut(s) 490, 915
BsuRI GGCC 2 cut(s) 576, 901
BsuTUI ATCGAT 1 cut(s) 119
BtgI CCRYGG 1 cut(s) 345
BtgZI GCGATG 1 cut(s) 140
BtsCI GGATG 4 cut(s) 244, 355, 446, 969
BtsIMutI CAGTG 2 cut(s) 386, 872
Cac8I GCNNGC 5 cut(s) 469, 613, 658, 780, 803
CciI TCATGA 1 cut(s) 48
CfoI GCGC 4 cut(s) 174, 186, 662, 1055
Cfr10I RCCGGY 2 cut(s) 467, 656
Cfr13I GGNCC 1 cut(s) 575
ClaI ATCGAT 1 cut(s) 119
CseI GACGC 1 cut(s) 1036
Csp6I GTAC 2 cut(s) 695, 946
CviAII CATG 7 cut(s) 49, 255, 518, 533, 581, 878, 975
CviQI GTAC 2 cut(s) 695, 946
DdeI CTNAG 1 cut(s) 571
DpnI GATC 6 cut(s) 83, 398, 421, 743, 761, 848
DpnII GATC 6 cut(s) 81, 396, 419, 741, 759, 846
DraIII CACNNNGTG 1 cut(s) 381
EaeI YGGCCR 1 cut(s) 899
Eam1104I CTCTTC 1 cut(s) 878
EarI CTCTTC 1 cut(s) 878
Eco31I GGTCTC 1 cut(s) 244
Eco32I GATATC 1 cut(s) 117
Eco81I CCTNAGG 1 cut(s) 571
EcoO109I RGGNCCY 1 cut(s) 575
EcoRI GAATTC 1 cut(s) 267
EcoRII CCWGG 2 cut(s) 285, 812
EcoRV GATATC 1 cut(s) 117
FaeI CATG 7 cut(s) 52, 258, 521, 536, 584, 881, 978
FatI CATG 7 cut(s) 48, 254, 517, 532, 580, 877, 974
FauNDI CATATG 1 cut(s) 931
FbaI TGATCA 2 cut(s) 396, 741
FblI GTMKAC 1 cut(s) 961
Fnu4HI GCNGC 2 cut(s) 41, 358
FokI GGATG 4 cut(s) 251, 362, 453, 956
Fsp4HI GCNGC 2 cut(s) 41, 358
FspBI CTAG 1 cut(s) 914
GlaI GCGC 4 cut(s) 173, 185, 661, 1054
GluI GCNGC 2 cut(s) 41, 358
GsuI CTGGAG 1 cut(s) 109
HaeII RGCGCY 1 cut(s) 663
HaeIII GGCC 2 cut(s) 576, 901
HapII CCGG 3 cut(s) 275, 468, 657
HgaI GACGC 1 cut(s) 1036
HhaI GCGC 4 cut(s) 174, 186, 662, 1055
Hin1II CATG 7 cut(s) 52, 258, 521, 536, 584, 881, 978
Hin6I GCGC 4 cut(s) 172, 184, 660, 1053
HinP1I GCGC 4 cut(s) 172, 184, 660, 1053
HinfI GANTC 6 cut(s) 130, 142, 523, 551, 822, 1040
HpaII CCGG 3 cut(s) 275, 468, 657
HphI GGTGA 2 cut(s) 151, 263
Hpy166II GTNNAC 3 cut(s) 384, 843, 962
Hpy188I TCNGA 3 cut(s) 741, 821, 827
Hpy188III TCNNGA 3 cut(s) 49, 646, 914
Hpy8I GTNNAC 3 cut(s) 384, 843, 962
Hpy99I CGWCG 1 cut(s) 1030
HpyAV CCTTC 2 cut(s) 537, 677
HpyCH4III ACNGT 1 cut(s) 381
HpyCH4IV ACGT 1 cut(s) 215
HpyCH4V TGCA 5 cut(s) 105, 153, 221, 754, 967
HpyF10VI GCNNNNNNNGC 3 cut(s) 192, 788, 898
HpyF3I CTNAG 1 cut(s) 571
HpySE526I ACGT 1 cut(s) 215
Hsp92II CATG 7 cut(s) 52, 258, 521, 536, 584, 881, 978
HspAI GCGC 4 cut(s) 172, 184, 660, 1053
KroI GCCGGC 2 cut(s) 467, 656
KroNI GCCGGC 2 cut(s) 469, 658
Ksp22I TGATCA 2 cut(s) 396, 741
Kzo9I GATC 6 cut(s) 81, 396, 419, 741, 759, 846
LguI GCTCTTC 1 cut(s) 878
LmnI GCTCC 2 cut(s) 288, 940
Lsp1109I GCAGC 2 cut(s) 52, 369
LweI GCATC 1 cut(s) 431
MaeI CTAG 1 cut(s) 914
MaeII ACGT 1 cut(s) 215
MaeIII GTNAC 3 cut(s) 74, 188, 520
MalI GATC 6 cut(s) 83, 398, 421, 743, 761, 848
MboI GATC 6 cut(s) 81, 396, 419, 741, 759, 846
MboII GAAGA 7 cut(s) 24, 173, 534, 622, 676, 826, 865
MhlI GDGCHC 1 cut(s) 318
MluCI AATT 4 cut(s) 267, 402, 639, 733
MlyI GAGTC 2 cut(s) 124, 517
MnlI CCTC 9 cut(s) 84, 229, 269, 347, 484, 530, 580, 603, 1050
MroNI GCCGGC 2 cut(s) 467, 656
MslI CAYNNNNRTG 2 cut(s) 749, 882
MspA1I CMGCKG 2 cut(s) 195, 940
MspI CCGG 3 cut(s) 275, 468, 657
MspR9I CCNGG 3 cut(s) 275, 287, 814
Mva1269I GAATGC 1 cut(s) 658
MvaI CCWGG 2 cut(s) 287, 814
MvnI CGCG 5 cut(s) 172, 186, 791, 1030, 1055
MwoI GCNNNNNNNGC 3 cut(s) 192, 788, 898
NaeI GCCGGC 2 cut(s) 469, 658
NciI CCSGG 1 cut(s) 275
NdeI CATATG 1 cut(s) 931
NdeII GATC 6 cut(s) 81, 396, 419, 741, 759, 846
NgoMIV GCCGGC 2 cut(s) 467, 656
NlaIII CATG 7 cut(s) 52, 258, 521, 536, 584, 881, 978
NlaIV GGNNCC 3 cut(s) 284, 577, 818
NmuCI GTSAC 1 cut(s) 520
PagI TCATGA 1 cut(s) 48
PciSI GCTCTTC 1 cut(s) 878
PctI GAATGC 1 cut(s) 658
PdiI GCCGGC 2 cut(s) 469, 658
PfeI GAWTC 4 cut(s) 142, 551, 822, 1040
PkrI GCNGC 2 cut(s) 42, 359
Ple19I CGATCG 1 cut(s) 849
PleI GAGTC 2 cut(s) 124, 517
PpsI GAGTC 2 cut(s) 124, 517
PsiI TTATAA 1 cut(s) 11
Psp6I CCWGG 2 cut(s) 285, 812
PspGI CCWGG 2 cut(s) 285, 812
PspN4I GGNNCC 3 cut(s) 284, 577, 818
PspPI GGNCC 1 cut(s) 575
PstI CTGCAG 1 cut(s) 223
PvuI CGATCG 1 cut(s) 849
RsaI GTAC 2 cut(s) 696, 947
RsaNI GTAC 2 cut(s) 695, 946
RseI CAYNNNNRTG 2 cut(s) 749, 882
SapI GCTCTTC 1 cut(s) 878
SatI GCNGC 2 cut(s) 41, 358
Sau3AI GATC 6 cut(s) 81, 396, 419, 741, 759, 846
Sau96I GGNCC 1 cut(s) 575
SchI GAGTC 2 cut(s) 124, 517
ScrFI CCNGG 3 cut(s) 275, 287, 814
SduI GDGCHC 1 cut(s) 318
SetI ASST 9 cut(s) 31, 218, 495, 541, 595, 803, 894, 1008, 1061
SfaNI GCATC 1 cut(s) 431
SfcI CTRYAG 2 cut(s) 219, 979
SmiMI CAYNNNNRTG 2 cut(s) 749, 882
SmlI CTYRAG 2 cut(s) 260, 594
SmoI CTYRAG 2 cut(s) 260, 594
Sse9I AATT 4 cut(s) 267, 402, 639, 733
SsiI CCGC 8 cut(s) 56, 170, 186, 195, 339, 448, 789, 938
SspMI CTAG 1 cut(s) 914
StyD4I CCNGG 3 cut(s) 273, 285, 812
TaaI ACNGT 1 cut(s) 381
TaiI ACGT 1 cut(s) 218
TaqI TCGA 3 cut(s) 119, 585, 1025
TaqII GACCGA 2 cut(s) 943, 947
TasI AATT 4 cut(s) 267, 402, 639, 733
TfiI GAWTC 4 cut(s) 142, 551, 822, 1040
TscAI CASTG 2 cut(s) 386, 872
TseFI GTSAC 1 cut(s) 520
TseI GCWGC 2 cut(s) 40, 357
Tsp45I GTSAC 1 cut(s) 520
TspDTI ATGAA 3 cut(s) 298, 665, 1016
TspGWI ACGGA 2 cut(s) 334, 689
TspRI CASTG 2 cut(s) 386, 872
XapI RAATTY 2 cut(s) 267, 733
XbaI TCTAGA 1 cut(s) 913
XmiI GTMKAC 1 cut(s) 961
XspI CTAG 1 cut(s) 914
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.