AT5G64730

WD repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005405
Physical Location & Seq
Forward (+)
25873101 .. 25875293
2193 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G64730.1

Sequence Viewer

Length: 900 bp
ATGAGCGCGACGGAGCTGCCGACGAAGGAGGCACACATACTTAAAGGCCACGAGGGGGCGGTTTTAGCGGCGAGGTTTAACGGTGACGGAAACTACGCTCTCACCTGTGGCAAGGATCGAACTATCCGCCTCTGGAACCCGCACCGTGGAATCTTAATCAAGACCTACAAATCTCATGGCCGCGAAGTCCGCGACGTTCATGTTACTTCAGACAATGCCAAATTTTGCTCATGTGGTGGTGATCGGCAAGTTTACTACTGGGATGTTTCAACTGGACGTGTAATCCGTAAATTCCGTGGTCATGATGGAGAGGTCAATGCAGTGAAGTTCAATGATTCGTCGTCTGTGGTTGTATCAGCTGGGTTCGATCGTTCGCTACGTGTATGGGACTGCAGATCTCACAGTGTTGAGCCAGTACAGATCATTGATACATTTTTGGATACAGTCATGTCTGTTGTTTTAACAAAGACTGAGATTATTGGTGGTAGTGTCGATGGAACAGTTCGCACATTTGACATGCGTATCGGTAGGGAGATGTCAGATAACCTAGGCCAACCGGTTAACTGTATATCGATATCAAATGATGGAAACTGCGTTTTAGCTGGTTGCTTGGATTCTACTCTGCGTCTACTTGACAGGACCACAGGAGAGCTACTCCAAGTCTATAAAGGTCATATTTCCAAGTCATTTAAAACGGATTGTTGCCTCACGAATTCGGATGCACATGTGATTGGAGGATCAGAGGATGGATTGGTTTTCTTTTGGGATTTGGTAGATGCAAAAGTGCTATCGAAATTTAGAGCTCACGATTTAGTGGTGACAAGCGTGAGTTACCACCCGAAGGAAGACTGTATGTTGACTTCTTCGGTCGATGGTACAATTCGTGTCTGGAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

299

Amino Acids

33.07

Weight (kDa)

7.14

Isoelectric Point (pI)

29.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WDR55 PF24796 7 - 183 8.9e-15 WDR55
Beta-prop_TEP1_2nd PF25047 9 - 133 9.3e-16 TEP-1 second beta-propeller
WD40 PF00400 10 - 46 6.8e-09 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 11 - 74 9.8e-09 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 11 - 136 9e-13 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 11 - 77 7.9e-07 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 13 - 130 8.4e-19 WDR3 second beta-propeller domain
EIF3I PF24805 13 - 224 6.4e-21 EIF3I
Beta-prop_WDR5 PF25175 14 - 298 8.5e-63 WDR5 beta-propeller domain
Beta-prop_EML PF23409 14 - 144 3.9e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 15 - 146 3.7e-13 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 17 - 96 2.7e-12 THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 29 - 129 1e-07 MABP1/WDR62 second WD40 domain
Beta-prop_SCAP PF24017 34 - 135 2.7e-07 SCAP Beta-propeller
Beta-prop_SPT8 PF23798 50 - 111 1.4e-07 SPT8 beta propeller
Beta-prop_CAF1B_HIR1 PF24105 52 - 136 6.1e-07 CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz PF24807 53 - 135 3.2e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR41 PF25178 54 - 158 6e-10 WDR41
Beta-prop_WDR3_1st PF25173 55 - 172 2.7e-16 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 59 - 298 7.1e-29 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 62 - 136 1.4e-08 WDHD1 first WD40 domain
WD40_Prp19 PF24814 70 - 225 3e-19 Prp19 WD40 domain
WD40 PF00400 93 - 130 6.9e-09 WD domain, G-beta repeat
WD40_MABP1-WDR62_2nd PF24782 99 - 257 2.3e-07 MABP1/WDR62 second WD40 domain
WD40_WDHD1_1st PF24817 147 - 266 4.8e-08 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 155 - 292 1.7e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 155 - 298 4.9e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_ACSF4 PF13570 155 - 296 1.9e-10 Beta-alanine-activating enzyme, beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 159 - 297 8.8e-11 WDR36/Utp21 first beta-propeller
WD40_Gbeta PF25391 169 - 296 4.2e-07 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 184 - 297 1.8e-16 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 184 - 298 3.9e-10 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 185 - 298 5.6e-10 TEP-1 second beta-propeller
WDR55 PF24796 185 - 297 2.3e-11 WDR55
WD40_Prp19 PF24814 187 - 297 5.8e-15 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 187 - 298 5e-09 WDR3 second beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 219 - 298 3.1e-07 CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st PF24817 233 - 298 7.6e-08 WDHD1 first WD40 domain
WD40 PF00400 261 - 297 1.9e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 628
AccII CGCG 3 cut(s) 8, 183, 192
AciI CCGC 6 cut(s) 59, 68, 127, 140, 181, 190
AclWI GGATC 2 cut(s) 123, 745
AcoI YGGCCR 1 cut(s) 178
AcsI RAATTY 4 cut(s) 221, 290, 712, 794
AcuI CTGAAG 1 cut(s) 192
AfaI GTAC 2 cut(s) 417, 877
AfiI CCNNNNNNNGG 3 cut(s) 55, 146, 841
AflIII ACRYGT 3 cut(s) 277, 379, 724
AgeI ACCGGT 1 cut(s) 556
AgsI TTSAA 2 cut(s) 270, 331
AjiI CACGTC 1 cut(s) 278
AluBI AGCT 5 cut(s) 16, 359, 602, 652, 803
AluI AGCT 5 cut(s) 16, 359, 602, 652, 803
Alw21I GWGCWC 1 cut(s) 805
AlwI GGATC 2 cut(s) 123, 745
AoxI GGCC 3 cut(s) 46, 178, 550
ApeKI GCWGC 1 cut(s) 16
ApoI RAATTY 4 cut(s) 221, 290, 712, 794
AsiGI ACCGGT 1 cut(s) 556
AspA2I CCTAGG 1 cut(s) 547
AspLEI GCGC 1 cut(s) 8
AspS9I GGNCC 1 cut(s) 639
AsuHPI GGTGA 4 cut(s) 94, 95, 251, 829
AvaII GGWCC 1 cut(s) 639
AvrII CCTAGG 1 cut(s) 547
BaeI ACNNNNGTAYC 2 cut(s) 505, 538
BanII GRGCYC 1 cut(s) 805
BarI GAAGNNNNNNTAC 2 cut(s) 844, 876
BauI CACGAG 1 cut(s) 50
BbsI GAAGAC 1 cut(s) 852
Bbv12I GWGCWC 1 cut(s) 805
BbvI GCAGC 1 cut(s) 3
BccI CCATC 5 cut(s) 299, 488, 578, 740, 866
BcgI CGANNNNNNTGC 1 cut(s) 32
BciVI GTATCC 1 cut(s) 433
BfaI CTAG 1 cut(s) 548
BfmI CTRYAG 1 cut(s) 391
BfuI GTATCC 1 cut(s) 433
BglII AGATCT 1 cut(s) 395
BisI GCNGC 3 cut(s) 17, 69, 181
BlnI CCTAGG 1 cut(s) 547
BlsI GCNGC 3 cut(s) 18, 70, 182
Bme18I GGWCC 1 cut(s) 639
BmgBI CACGTC 1 cut(s) 278
BmgT120I GGNCC 1 cut(s) 639
BmiI GGNNCC 1 cut(s) 137
BmrI ACTGGG 1 cut(s) 268
BmsI GCATC 2 cut(s) 709, 766
BmuI ACTGGG 1 cut(s) 268
BpiI GAAGAC 1 cut(s) 852
BplI GAGNNNNNCTC 2 cut(s) 639, 671
Bsa29I ATCGAT 1 cut(s) 572
BsaAI YACGTR 1 cut(s) 380
BsaJI CCNNGG 3 cut(s) 145, 295, 547
BsaWI WCCGGW 1 cut(s) 556
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 146, 841
Bse118I RCCGGY 1 cut(s) 556
Bse1I ACTGG 3 cut(s) 263, 277, 413
BseCI ATCGAT 1 cut(s) 572
BseDI CCNNGG 3 cut(s) 145, 295, 547
BseGI GGATG 3 cut(s) 268, 724, 751
BseLI CCNNNNNNNGG 3 cut(s) 55, 146, 841
BseMII CTCAG 1 cut(s) 462
BseNI ACTGG 3 cut(s) 263, 277, 413
BseXI GCAGC 1 cut(s) 3
BseYI CCCAGC 1 cut(s) 359
Bsh1236I CGCG 3 cut(s) 8, 183, 192
Bsh1285I CGRYCG 2 cut(s) 370, 870
BshFI GGCC 3 cut(s) 48, 180, 552
BshTI ACCGGT 1 cut(s) 556
BshVI ATCGAT 1 cut(s) 572
BsiEI CGRYCG 2 cut(s) 370, 870
BsiHKAI GWGCWC 1 cut(s) 805
BsiSI CCGG 1 cut(s) 557
BslFI GGGAC 1 cut(s) 401
BslI CCNNNNNNNGG 3 cut(s) 55, 146, 841
BsmFI GGGAC 1 cut(s) 401
BsnI GGCC 3 cut(s) 48, 180, 552
Bsp1286I GDGCHC 1 cut(s) 805
Bsp143I GATC 6 cut(s) 115, 241, 367, 395, 420, 737
BspACI CCGC 6 cut(s) 59, 68, 127, 140, 181, 190
BspANI GGCC 3 cut(s) 48, 180, 552
BspCNI CTCAG 1 cut(s) 463
BspDI ATCGAT 1 cut(s) 572
BspFNI CGCG 3 cut(s) 8, 183, 192
BspHI TCATGA 1 cut(s) 301
BspLI GGNNCC 1 cut(s) 137
BspMAI CTGCAG 1 cut(s) 395
BspPI GGATC 2 cut(s) 123, 745
BsrFI RCCGGY 1 cut(s) 556
BsrI ACTGG 3 cut(s) 263, 277, 413
BssAI RCCGGY 1 cut(s) 556
BssECI CCNNGG 3 cut(s) 145, 295, 547
BssMI GATC 6 cut(s) 115, 241, 367, 395, 420, 737
BssSI CACGAG 1 cut(s) 50
BssT1I CCWWGG 1 cut(s) 547
Bst2BI CACGAG 1 cut(s) 50
Bst4CI ACNGT 7 cut(s) 83, 146, 404, 445, 502, 566, 851
BstBAI YACGTR 1 cut(s) 380
BstDEI CTNAG 1 cut(s) 471
BstDSI CCRYGG 2 cut(s) 145, 295
BstF5I GGATG 3 cut(s) 268, 724, 751
BstFNI CGCG 3 cut(s) 8, 183, 192
BstHHI GCGC 1 cut(s) 8
BstKTI GATC 6 cut(s) 118, 244, 370, 398, 423, 740
BstMBI GATC 6 cut(s) 115, 241, 367, 395, 420, 737
BstMCI CGRYCG 2 cut(s) 370, 870
BstMWI GCNNNNNNNGC 2 cut(s) 65, 189
BstNSI RCATGY 2 cut(s) 520, 728
BstSFI CTRYAG 1 cut(s) 391
BstUI CGCG 3 cut(s) 8, 183, 192
BstV1I GCAGC 1 cut(s) 3
BstV2I GAAGAC 1 cut(s) 852
BstX2I RGATCY 1 cut(s) 395
BstYI RGATCY 1 cut(s) 395
Bsu15I ATCGAT 1 cut(s) 572
BsuI GTATCC 1 cut(s) 433
BsuRI GGCC 3 cut(s) 48, 180, 552
BsuTUI ATCGAT 1 cut(s) 572
BtgI CCRYGG 2 cut(s) 145, 295
BtrI CACGTC 1 cut(s) 278
BtsCI GGATG 3 cut(s) 268, 724, 751
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 2 cut(s) 327, 409
CciI TCATGA 1 cut(s) 301
CfoI GCGC 1 cut(s) 8
Cfr10I RCCGGY 1 cut(s) 556
Cfr13I GGNCC 1 cut(s) 639
ClaI ATCGAT 1 cut(s) 572
CseI GACGC 1 cut(s) 614
Csp6I GTAC 2 cut(s) 416, 876
CspAI ACCGGT 1 cut(s) 556
CviAII CATG 7 cut(s) 176, 200, 231, 302, 448, 517, 725
CviJI RGCY 9 cut(s) 16, 48, 180, 359, 412, 552, 602, 652, 803
CviKI_1 RGCY 9 cut(s) 16, 48, 180, 359, 412, 552, 602, 652, 803
CviQI GTAC 2 cut(s) 416, 876
DdeI CTNAG 1 cut(s) 471
DpnI GATC 6 cut(s) 117, 243, 369, 397, 422, 739
DpnII GATC 6 cut(s) 115, 241, 367, 395, 420, 737
DraI TTTAAA 1 cut(s) 691
EaeI YGGCCR 1 cut(s) 178
EciI GGCGGA 1 cut(s) 116
Ecl136II GAGCTC 1 cut(s) 803
Eco130I CCWWGG 1 cut(s) 547
Eco24I GRGCYC 1 cut(s) 805
Eco32I GATATC 1 cut(s) 576
Eco47I GGWCC 1 cut(s) 639
Eco53kI GAGCTC 1 cut(s) 803
Eco57I CTGAAG 1 cut(s) 192
EcoICRI GAGCTC 1 cut(s) 803
EcoRI GAATTC 1 cut(s) 712
EcoRV GATATC 1 cut(s) 576
EcoT14I CCWWGG 1 cut(s) 547
EcoT38I GRGCYC 1 cut(s) 805
ErhI CCWWGG 1 cut(s) 547
FaeI CATG 7 cut(s) 179, 203, 234, 305, 451, 520, 728
FaqI GGGAC 1 cut(s) 401
FatI CATG 7 cut(s) 175, 199, 230, 301, 447, 516, 724
FauI CCCGC 1 cut(s) 147
FblI GTMKAC 1 cut(s) 628
Fnu4HI GCNGC 3 cut(s) 17, 69, 181
FokI GGATG 3 cut(s) 275, 731, 758
FriOI GRGCYC 1 cut(s) 805
Fsp4HI GCNGC 3 cut(s) 17, 69, 181
FspBI CTAG 1 cut(s) 548
GlaI GCGC 1 cut(s) 7
GluI GCNGC 3 cut(s) 17, 69, 181
GsaI CCCAGC 1 cut(s) 363
HaeIII GGCC 3 cut(s) 48, 180, 552
HapII CCGG 1 cut(s) 557
HgaI GACGC 1 cut(s) 614
HhaI GCGC 1 cut(s) 8
Hin1II CATG 7 cut(s) 179, 203, 234, 305, 451, 520, 728
Hin6I GCGC 1 cut(s) 6
HinP1I GCGC 1 cut(s) 6
HincII GTYRAC 2 cut(s) 562, 858
HindII GTYRAC 2 cut(s) 562, 858
HinfI GANTC 3 cut(s) 150, 335, 614
HpaI GTTAAC 1 cut(s) 562
HpaII CCGG 1 cut(s) 557
HphI GGTGA 4 cut(s) 94, 95, 251, 829
Hpy166II GTNNAC 4 cut(s) 253, 562, 629, 858
Hpy188I TCNGA 4 cut(s) 211, 541, 718, 742
Hpy188III TCNNGA 6 cut(s) 133, 160, 302, 709, 806, 889
Hpy8I GTNNAC 4 cut(s) 253, 562, 629, 858
Hpy99I CGWCG 4 cut(s) 13, 25, 197, 343
HpyAV CCTTC 2 cut(s) 19, 835
HpyCH4III ACNGT 7 cut(s) 83, 146, 404, 445, 502, 566, 851
HpyCH4IV ACGT 3 cut(s) 195, 277, 379
HpyCH4V TGCA 4 cut(s) 320, 393, 722, 779
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 189
HpyF3I CTNAG 1 cut(s) 471
HpySE526I ACGT 3 cut(s) 195, 277, 379
Hsp92II CATG 7 cut(s) 179, 203, 234, 305, 451, 520, 728
HspAI GCGC 1 cut(s) 6
KspAI GTTAAC 1 cut(s) 562
Kzo9I GATC 6 cut(s) 115, 241, 367, 395, 420, 737
LmnI GCTCC 1 cut(s) 13
Lsp1109I GCAGC 1 cut(s) 3
LweI GCATC 2 cut(s) 709, 766
MaeI CTAG 1 cut(s) 548
MaeII ACGT 3 cut(s) 195, 277, 379
MaeIII GTNAC 4 cut(s) 83, 202, 817, 830
MalI GATC 6 cut(s) 117, 243, 369, 397, 422, 739
MboI GATC 6 cut(s) 115, 241, 367, 395, 420, 737
MboII GAAGA 2 cut(s) 855, 857
MflI RGATCY 1 cut(s) 395
MhlI GDGCHC 1 cut(s) 805
MluCI AATT 5 cut(s) 221, 290, 712, 794, 879
MnlI CCTC 8 cut(s) 22, 46, 66, 140, 304, 716, 728, 736
MseI TTAA 6 cut(s) 42, 78, 155, 461, 561, 690
MspA1I CMGCKG 1 cut(s) 359
MspI CCGG 1 cut(s) 557
MvnI CGCG 3 cut(s) 8, 183, 192
MwoI GCNNNNNNNGC 2 cut(s) 65, 189
NdeII GATC 6 cut(s) 115, 241, 367, 395, 420, 737
NlaIII CATG 7 cut(s) 179, 203, 234, 305, 451, 520, 728
NlaIV GGNNCC 1 cut(s) 137
NmuCI GTSAC 2 cut(s) 83, 817
NspI RCATGY 2 cut(s) 520, 728
PagI TCATGA 1 cut(s) 301
PciI ACATGT 1 cut(s) 724
PcsI WCGNNNNNNNCGW 3 cut(s) 17, 283, 376
PfeI GAWTC 3 cut(s) 150, 335, 614
PinAI ACCGGT 1 cut(s) 556
PkrI GCNGC 3 cut(s) 18, 70, 182
Ple19I CGATCG 1 cut(s) 370
Ppu21I YACGTR 1 cut(s) 380
PscI ACATGT 1 cut(s) 724
Psp124BI GAGCTC 1 cut(s) 805
PspFI CCCAGC 1 cut(s) 359
PspN4I GGNNCC 1 cut(s) 137
PspPI GGNCC 1 cut(s) 639
PstI CTGCAG 1 cut(s) 395
PsuI RGATCY 1 cut(s) 395
PvuI CGATCG 1 cut(s) 370
PvuII CAGCTG 1 cut(s) 359
RsaI GTAC 2 cut(s) 417, 877
RsaNI GTAC 2 cut(s) 416, 876
SacI GAGCTC 1 cut(s) 805
SaqAI TTAA 6 cut(s) 42, 78, 155, 461, 561, 690
SatI GCNGC 3 cut(s) 17, 69, 181
Sau3AI GATC 6 cut(s) 115, 241, 367, 395, 420, 737
Sau96I GGNCC 1 cut(s) 639
SduI GDGCHC 1 cut(s) 805
SfaNI GCATC 2 cut(s) 709, 766
SfcI CTRYAG 1 cut(s) 391
SinI GGWCC 1 cut(s) 639
Sse9I AATT 5 cut(s) 221, 290, 712, 794, 879
SsiI CCGC 6 cut(s) 59, 68, 127, 140, 181, 190
SspMI CTAG 1 cut(s) 548
SstI GAGCTC 1 cut(s) 805
StyI CCWWGG 1 cut(s) 547
TaaI ACNGT 7 cut(s) 83, 146, 404, 445, 502, 566, 851
TaiI ACGT 3 cut(s) 198, 280, 382
TaqI TCGA 6 cut(s) 118, 366, 492, 572, 791, 870
TaqII GACCGA 1 cut(s) 856
TasI AATT 5 cut(s) 221, 290, 712, 794, 879
TatI WGTACW 1 cut(s) 415
TauI GCSGC 2 cut(s) 71, 183
TfiI GAWTC 3 cut(s) 150, 335, 614
Tru1I TTAA 6 cut(s) 42, 78, 155, 461, 561, 690
Tru9I TTAA 6 cut(s) 42, 78, 155, 461, 561, 690
TscAI CASTG 2 cut(s) 327, 409
TseFI GTSAC 2 cut(s) 83, 817
TseI GCWGC 1 cut(s) 16
Tsp45I GTSAC 2 cut(s) 83, 817
TspDTI ATGAA 1 cut(s) 188
TspGWI ACGGA 5 cut(s) 26, 102, 275, 284, 710
TspRI CASTG 2 cut(s) 327, 409
VpaK11BI GGWCC 1 cut(s) 639
XapI RAATTY 4 cut(s) 221, 290, 712, 794
XceI RCATGY 2 cut(s) 520, 728
XmaJI CCTAGG 1 cut(s) 547
XmiI GTMKAC 1 cut(s) 628
XspI CTAG 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.