pycom01g03350

WD repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0005405
Physical Location & Seq
Forward (+)
2597949 .. 2601053
3105 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g03350.4

Sequence Viewer

Length: 900 bp
ATGAGCGAATCGGAATTGCCAACGAAAGAGGCAATCGTGCTGAGAGGGCACGAGGGCGCTGTATTAGCCGCCAGGTTCAACAGCGACGGAAACTACTGCCTGAGCGCCGGCAAAGACCGAACCATCCGACTATGGAACCCTCACCGCGGCATCCACATCAAGACCTACAAATCCCATGGCCGTGAAGTCCGCGACGTTCATGTGACCTCGGACAATTCCAAATTGTGTTCCTGCGGAGGTGATAGACAAATATTTTACTGGGATGTGGCCACGGGTCGAGTGATTCGAAAGTTTCGGGGACACGAAGGGGAGGTGAATGCTGTCAAGTTCAATGAGTATTCGTCGGTTGTTGTCTCCGCTGGTTTCGATCAGTCGTTGCGTGCTTGGGACTGCAGATCTCACAGCATTGAACCAATTCAGATAATTGACACATTTACAGATAGTGTAATGTCTGTTTGTTTGACAAAGACCGAGATTATTGGTGCAAGTGTTGATGGAACTGTTCGAACATTTGACATGCGTATTGGGAGAGAACTATCCGATGACTTGGGGCAACCTGTAAACTGCATCTCAATGTCAAATGATGGTAACTGCATACTGGCTGGCTGCTTAGATTCAACTTTACGCCTTATTGACAGAACTTCTGGTGAATTGCTTCAAGAATACAAAGGACACACTTGTAAGTCTTACAAATTGGACTGCTGCCTTACCAATACCGATGCCCATGTAACTGGTGGGTCGGAGGATGGTTACATTTTCTTCTGGGATCTGGTAGATGCATCTGTGGCAACAAGTTTCAGAGCGCATTCATCAGTGGTCACAAGTGTCAGTTATCACCCAAAGGACAACTGCATGATAAGTTCGTCTGTTGATGGCACCATTAGGGTGTGGAAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

300

Amino Acids

33.08

Weight (kDa)

6.12

Isoelectric Point (pI)

33.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 875
AccII CGCG 2 cut(s) 147, 192
AciI CCGC 6 cut(s) 69, 145, 147, 190, 234, 357
AclWI GGATC 1 cut(s) 774
AcoI YGGCCR 2 cut(s) 178, 267
AfiI CCNNNNNNNGG 1 cut(s) 146
AgsI TTSAA 5 cut(s) 79, 331, 410, 618, 659
AjnI CCWGG 1 cut(s) 71
Alw26I GTCTC 1 cut(s) 358
AlwI GGATC 1 cut(s) 774
AoxI GGCC 2 cut(s) 178, 267
ApeKI GCWGC 2 cut(s) 606, 702
Asp700I GAANNNNTTC 2 cut(s) 414, 654
AspLEI GCGC 3 cut(s) 59, 107, 805
AsuHPI GGTGA 5 cut(s) 134, 251, 325, 659, 827
AsuII TTCGAA 2 cut(s) 286, 505
BaeGI GKGCMC 1 cut(s) 51
BalI TGGCCA 1 cut(s) 269
BanI GGYRCC 1 cut(s) 875
BauI CACGAG 1 cut(s) 50
BbvI GCAGC 2 cut(s) 593, 689
BccI CCATC 5 cut(s) 131, 488, 578, 740, 866
BceAI ACGGC 1 cut(s) 165
BciT130I CCWGG 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 358
BfmI CTRYAG 1 cut(s) 391
BfoI RGCGCY 2 cut(s) 60, 108
BglII AGATCT 1 cut(s) 395
BisI GCNGC 4 cut(s) 69, 148, 607, 703
BlsI GCNGC 4 cut(s) 70, 149, 608, 704
Bme1390I CCNGG 1 cut(s) 73
BmiI GGNNCC 2 cut(s) 137, 877
BmrFI CCNGG 1 cut(s) 73
BmrI ACTGGG 1 cut(s) 268
BmsI GCATC 5 cut(s) 159, 576, 709, 766, 788
BmuI ACTGGG 1 cut(s) 268
Bpu10I CCTNAGC 1 cut(s) 101
Bpu14I TTCGAA 2 cut(s) 286, 505
BsaJI CCNNGG 4 cut(s) 145, 175, 207, 270
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse118I RCCGGY 1 cut(s) 107
Bse1I ACTGG 3 cut(s) 263, 603, 736
BseBI CCWGG 1 cut(s) 73
BseDI CCNNGG 4 cut(s) 145, 175, 207, 270
BseGI GGATG 4 cut(s) 123, 150, 268, 751
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMII CTCAG 2 cut(s) 32, 92
BseNI ACTGG 3 cut(s) 263, 603, 736
BseSI GKGCMC 1 cut(s) 51
BseXI GCAGC 2 cut(s) 593, 689
Bsh1236I CGCG 2 cut(s) 147, 192
BshFI GGCC 2 cut(s) 180, 269
BshNI GGYRCC 1 cut(s) 875
BsiSI CCGG 1 cut(s) 108
BslFI GGGAC 2 cut(s) 312, 401
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 1 cut(s) 358
BsmFI GGGAC 2 cut(s) 312, 401
BsmI GAATGC 2 cut(s) 322, 805
BsnI GGCC 2 cut(s) 180, 269
Bsp119I TTCGAA 2 cut(s) 286, 505
Bsp1286I GDGCHC 1 cut(s) 51
Bsp143I GATC 3 cut(s) 367, 395, 766
Bsp19I CCATGG 1 cut(s) 175
BspACI CCGC 6 cut(s) 69, 145, 147, 190, 234, 357
BspANI GGCC 2 cut(s) 180, 269
BspCNI CTCAG 2 cut(s) 33, 93
BspFNI CGCG 2 cut(s) 147, 192
BspLI GGNNCC 2 cut(s) 137, 877
BspMAI CTGCAG 1 cut(s) 395
BspPI GGATC 1 cut(s) 774
BspT104I TTCGAA 2 cut(s) 286, 505
BspT107I GGYRCC 1 cut(s) 875
BsrFI RCCGGY 1 cut(s) 107
BsrI ACTGG 3 cut(s) 263, 603, 736
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 4 cut(s) 145, 175, 207, 270
BssMI GATC 3 cut(s) 367, 395, 766
BssSI CACGAG 1 cut(s) 50
BssT1I CCWWGG 1 cut(s) 175
Bst2BI CACGAG 1 cut(s) 50
Bst2UI CCWGG 1 cut(s) 73
Bst4CI ACNGT 1 cut(s) 502
BstBI TTCGAA 2 cut(s) 286, 505
BstC8I GCNNGC 3 cut(s) 109, 381, 604
BstDEI CTNAG 3 cut(s) 41, 101, 610
BstDSI CCRYGG 3 cut(s) 145, 175, 270
BstF5I GGATG 4 cut(s) 123, 150, 268, 751
BstFNI CGCG 2 cut(s) 147, 192
BstH2I RGCGCY 2 cut(s) 60, 108
BstHHI GCGC 3 cut(s) 59, 107, 805
BstKTI GATC 3 cut(s) 370, 398, 769
BstMAI GTCTC 1 cut(s) 358
BstMBI GATC 3 cut(s) 367, 395, 766
BstMWI GCNNNNNNNGC 3 cut(s) 46, 65, 785
BstNI CCWGG 1 cut(s) 73
BstNSI RCATGY 1 cut(s) 520
BstSCI CCNGG 1 cut(s) 71
BstSFI CTRYAG 1 cut(s) 391
BstSLI GKGCMC 1 cut(s) 51
BstUI CGCG 2 cut(s) 147, 192
BstV1I GCAGC 2 cut(s) 593, 689
BstX2I RGATCY 2 cut(s) 395, 766
BstXI CCANNNNNNTGG 1 cut(s) 731
BstYI RGATCY 2 cut(s) 395, 766
BsuRI GGCC 2 cut(s) 180, 269
BtgI CCRYGG 3 cut(s) 145, 175, 270
BtsCI GGATG 4 cut(s) 123, 150, 268, 751
BtsIMutI CAGTG 1 cut(s) 819
Cac8I GCNNGC 3 cut(s) 109, 381, 604
CfoI GCGC 3 cut(s) 59, 107, 805
Cfr10I RCCGGY 1 cut(s) 107
Cfr42I CCGCGG 1 cut(s) 148
CviAII CATG 5 cut(s) 176, 200, 517, 725, 853
CviJI RGCY 5 cut(s) 68, 180, 269, 602, 606
CviKI_1 RGCY 5 cut(s) 68, 180, 269, 602, 606
DdeI CTNAG 3 cut(s) 41, 101, 610
DpnI GATC 3 cut(s) 369, 397, 768
DpnII GATC 3 cut(s) 367, 395, 766
EaeI YGGCCR 2 cut(s) 178, 267
Eco130I CCWWGG 1 cut(s) 175
EcoRII CCWGG 1 cut(s) 71
EcoT14I CCWWGG 1 cut(s) 175
EcoT22I ATGCAT 1 cut(s) 781
ErhI CCWWGG 1 cut(s) 175
FaeI CATG 5 cut(s) 179, 203, 520, 728, 856
FaiI YATR 7 cut(s) 133, 177, 201, 518, 596, 726, 854
FaqI GGGAC 2 cut(s) 312, 401
FatI CATG 5 cut(s) 175, 199, 516, 724, 852
Fnu4HI GCNGC 4 cut(s) 69, 148, 607, 703
FokI GGATG 4 cut(s) 110, 137, 275, 758
Fsp4HI GCNGC 4 cut(s) 69, 148, 607, 703
GlaI GCGC 3 cut(s) 58, 106, 804
GluI GCNGC 4 cut(s) 69, 148, 607, 703
HaeII RGCGCY 2 cut(s) 60, 108
HaeIII GGCC 2 cut(s) 180, 269
HapII CCGG 1 cut(s) 108
HhaI GCGC 3 cut(s) 59, 107, 805
Hin1II CATG 5 cut(s) 179, 203, 520, 728, 856
Hin6I GCGC 3 cut(s) 57, 105, 803
HinP1I GCGC 3 cut(s) 57, 105, 803
HinfI GANTC 3 cut(s) 8, 283, 614
HpaII CCGG 1 cut(s) 108
HphI GGTGA 5 cut(s) 134, 251, 325, 659, 827
Hpy166II GTNNAC 1 cut(s) 562
Hpy188I TCNGA 7 cut(s) 13, 128, 211, 420, 541, 742, 800
Hpy188III TCNNGA 2 cut(s) 160, 659
Hpy8I GTNNAC 1 cut(s) 562
Hpy99I CGWCG 3 cut(s) 89, 197, 346
HpyAV CCTTC 1 cut(s) 299
HpyCH4III ACNGT 1 cut(s) 502
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 6 cut(s) 393, 485, 567, 594, 779, 852
HpyF10VI GCNNNNNNNGC 3 cut(s) 46, 65, 785
HpyF3I CTNAG 3 cut(s) 41, 101, 610
HpySE526I ACGT 1 cut(s) 195
Hsp92II CATG 5 cut(s) 179, 203, 520, 728, 856
HspAI GCGC 3 cut(s) 57, 105, 803
KroI GCCGGC 1 cut(s) 107
KroNI GCCGGC 1 cut(s) 109
KspI CCGCGG 1 cut(s) 148
Kzo9I GATC 3 cut(s) 367, 395, 766
Lsp1109I GCAGC 2 cut(s) 593, 689
LweI GCATC 5 cut(s) 159, 576, 709, 766, 788
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 5 cut(s) 202, 587, 727, 749, 817
MalI GATC 3 cut(s) 369, 397, 768
MboI GATC 3 cut(s) 367, 395, 766
MboII GAAGA 1 cut(s) 751
MflI RGATCY 2 cut(s) 395, 766
MhlI GDGCHC 1 cut(s) 51
MlsI TGGCCA 1 cut(s) 269
MluCI AATT 7 cut(s) 14, 214, 221, 414, 423, 650, 692
MluNI TGGCCA 1 cut(s) 269
MmeI TCCRAC 2 cut(s) 151, 720
MnlI CCTC 8 cut(s) 22, 38, 46, 150, 217, 230, 304, 736
Mox20I TGGCCA 1 cut(s) 269
Mph1103I ATGCAT 1 cut(s) 781
MroNI GCCGGC 1 cut(s) 107
MroXI GAANNNNTTC 2 cut(s) 414, 654
MscI TGGCCA 1 cut(s) 269
MslI CAYNNNNRTG 3 cut(s) 180, 572, 884
Msp20I TGGCCA 1 cut(s) 269
MspA1I CMGCKG 2 cut(s) 147, 359
MspI CCGG 1 cut(s) 108
MspR9I CCNGG 1 cut(s) 73
Mva1269I GAATGC 2 cut(s) 322, 805
MvaI CCWGG 1 cut(s) 73
MvnI CGCG 2 cut(s) 147, 192
MwoI GCNNNNNNNGC 3 cut(s) 46, 65, 785
NaeI GCCGGC 1 cut(s) 109
NcoI CCATGG 1 cut(s) 175
NdeII GATC 3 cut(s) 367, 395, 766
NgoMIV GCCGGC 1 cut(s) 107
NlaIII CATG 5 cut(s) 179, 203, 520, 728, 856
NlaIV GGNNCC 2 cut(s) 137, 877
NmuCI GTSAC 2 cut(s) 202, 817
NsiI ATGCAT 1 cut(s) 781
NspI RCATGY 1 cut(s) 520
NspV TTCGAA 2 cut(s) 286, 505
PcsI WCGNNNNNNNCGW 1 cut(s) 283
PctI GAATGC 2 cut(s) 322, 805
PdiI GCCGGC 1 cut(s) 109
PdmI GAANNNNTTC 2 cut(s) 414, 654
PfeI GAWTC 3 cut(s) 8, 283, 614
PkrI GCNGC 4 cut(s) 70, 149, 608, 704
Psp6I CCWGG 1 cut(s) 71
PspGI CCWGG 1 cut(s) 71
PspN4I GGNNCC 2 cut(s) 137, 877
PstI CTGCAG 1 cut(s) 395
PsuI RGATCY 2 cut(s) 395, 766
RseI CAYNNNNRTG 3 cut(s) 180, 572, 884
SacII CCGCGG 1 cut(s) 148
SatI GCNGC 4 cut(s) 69, 148, 607, 703
Sau3AI GATC 3 cut(s) 367, 395, 766
ScrFI CCNGG 1 cut(s) 73
SduI GDGCHC 1 cut(s) 51
SetI ASST 7 cut(s) 77, 167, 198, 209, 241, 315, 559
SfaNI GCATC 5 cut(s) 159, 576, 709, 766, 788
SfcI CTRYAG 1 cut(s) 391
Sfr303I CCGCGG 1 cut(s) 148
SfuI TTCGAA 2 cut(s) 286, 505
SgrBI CCGCGG 1 cut(s) 148
SmiMI CAYNNNNRTG 3 cut(s) 180, 572, 884
Sse9I AATT 7 cut(s) 14, 214, 221, 414, 423, 650, 692
SsiI CCGC 6 cut(s) 69, 145, 147, 190, 234, 357
SspI AATATT 1 cut(s) 252
StyD4I CCNGG 1 cut(s) 71
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 1 cut(s) 502
TaiI ACGT 1 cut(s) 198
TaqI TCGA 4 cut(s) 277, 286, 366, 505
TaqII GACCGA 2 cut(s) 132, 485
TasI AATT 7 cut(s) 14, 214, 221, 414, 423, 650, 692
TauI GCSGC 2 cut(s) 71, 150
TfiI GAWTC 3 cut(s) 8, 283, 614
TscAI CASTG 1 cut(s) 819
TseFI GTSAC 2 cut(s) 202, 817
TseI GCWGC 2 cut(s) 606, 702
Tsp45I GTSAC 2 cut(s) 202, 817
TspDTI ATGAA 2 cut(s) 188, 798
TspGWI ACGGA 1 cut(s) 102
TspRI CASTG 1 cut(s) 819
XceI RCATGY 1 cut(s) 520
XcmI CCANNNNNNNNNTGG 1 cut(s) 731
XmnI GAANNNNTTC 2 cut(s) 414, 654
Zsp2I ATGCAT 1 cut(s) 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.