Rroxscaffold_2G00124070

WD repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005405
Physical Location & Seq
Reverse (-)
58141076 .. 58145179
4104 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00124070.1

Sequence Viewer

Length: 900 bp
ATGAGTGTCTCAGATCTGCCAAAGAAGGAGGCAAATGTACTTAAAGGGCATGAGGGAGCAGTATTAGCAGCAAGGTTCAATAAAGACGGAAATTACTGCCTCAGCTGCGGGAAAGACCGAACCATCCGACTCTGGAACCCTCACCGTGGCATCCACATTAAGACATACAAGTCCCATGGCCGTGAAGTCCGTGATGTCCATGTCACCTCGGACAACTCCAAATTATGTTCAGGTGGTGGCGACAGACAAATCTTCTATTGGGATGTTGCATCCGGTCGAGTCATTCGAAAATTTCGGGGCCATGATGGAGGGGTGAATGCTGTGAAGTTCAATGAGTATGCTTCTGTTGTAGTCTCAGCAGGATATGATCAGTCATTGCGTGCTTGGGACTGCAGGTCTCACAGTACTGAGCCTATTCAGATAATTGACTCATTTACAGATAGTGTAATGTCTGTTTGTTTAACAAAGACTGAGATAATTGGTGGAAGTGTTGATGGAACTGTTCGAACATTTGACATTCGTATGGGAAGAGAACTATCAGATGACTTGGGACAACCTGTGAACTGTATCTCAATGTCAAATGATGGTAACTGCATATTAGCAAGCTGCTTAGATTCGACTATACGTCTCCTTGACAGGTCTTCTGCTGAATTACTTCAAGAATACAAAGGCCATACTTGTAAGTCTTACAAATTGGACTGTTGCCTTACCAACACGGATGCCCATGTAATCGGAGGATCTGAGGATGGTTCAATTTTCTTCTGGGATTTAGTAGATGCATCTGTTGTGTCAAGTATCCGAGCACATTCATCAGTGGTAACAAGTGTCAGTTATCATCCAAAGGACAACTGCATGATAAGTTCCTCTGTTGATGGCACCATTAAGGTGTGGAAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

299

Amino Acids

32.84

Weight (kDa)

6.82

Isoelectric Point (pI)

38.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WDR55 PF24796 9 - 181 4e-14 WDR55
WD40_Prp19 PF24814 9 - 68 4e-07 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 10 - 132 1.3e-13 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 11 - 96 5.1e-09 CDC20/Fizzy WD40 domain
WD40 PF00400 11 - 46 2.6e-09 WD domain, G-beta repeat
WD40_Gbeta PF25391 12 - 144 1.2e-12 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 12 - 74 6.8e-10 WDR3 first beta-propeller domain
EIF3I PF24805 13 - 225 3.2e-24 EIF3I
Beta-prop_EML PF23409 14 - 158 4.3e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5 PF25175 14 - 298 7.6e-66 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 14 - 89 8.3e-09 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 15 - 147 6e-12 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 17 - 95 4.7e-13 THOC3 beta-propeller domain
Beta-prop_SCAP PF24017 37 - 132 5.8e-07 SCAP Beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 40 - 129 1.6e-06 WDR36/Utp21 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 51 - 135 2e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_SPT8 PF23798 53 - 112 6.5e-06 SPT8 beta propeller
WD40_CDC20-Fz PF24807 53 - 134 3.1e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR41 PF25178 53 - 155 2.8e-06 WDR41
Beta-prop_WDR3_1st PF25173 55 - 173 1.5e-18 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 59 - 299 2.2e-31 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 62 - 137 3.1e-08 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 63 - 172 1.3e-10 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 70 - 196 2.7e-16 Prp19 WD40 domain
WD40 PF00400 93 - 130 1.6e-06 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 97 - 184 8.8e-07 TEP-1 second beta-propeller
WD40_MABP1-WDR62_2nd PF24782 97 - 257 3.3e-07 MABP1/WDR62 second WD40 domain
Beta-prop_EML_2 PF23414 112 - 260 6e-08 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 147 - 265 4.6e-08 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 155 - 291 1.1e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 155 - 298 1.3e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_ACSF4 PF13570 158 - 296 2.6e-08 Beta-alanine-activating enzyme, beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 159 - 297 2e-12 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_2nd PF25172 165 - 298 2.6e-12 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 184 - 298 1.4e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 185 - 298 2.1e-18 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 185 - 298 6.8e-10 TEP-1 second beta-propeller
WDR55 PF24796 186 - 298 2.1e-11 WDR55
WD40_Prp19 PF24814 187 - 297 1.5e-16 Prp19 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 192 - 298 9.7e-09 MABP1/WDR62 second WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 223 - 298 3.1e-06 CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st PF24817 235 - 298 2.2e-10 WDHD1 first WD40 domain
WD40 PF00400 262 - 297 4.6e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 169
Acc36I ACCTGC 1 cut(s) 384
AccB1I GGYRCC 1 cut(s) 875
AciI CCGC 1 cut(s) 108
AclWI GGATC 1 cut(s) 745
AcoI YGGCCR 1 cut(s) 178
AcsI RAATTY 1 cut(s) 290
AfaI GTAC 2 cut(s) 39, 406
AfiI CCNNNNNNNGG 1 cut(s) 146
AgsI TTSAA 4 cut(s) 79, 331, 659, 753
AhdI GACNNNNNGTC 2 cut(s) 394, 624
AluBI AGCT 2 cut(s) 105, 606
AluI AGCT 2 cut(s) 105, 606
Alw21I GWGCWC 1 cut(s) 805
Alw26I GTCTC 4 cut(s) 13, 358, 402, 632
AlwI GGATC 1 cut(s) 745
AoxI GGCC 3 cut(s) 178, 298, 670
ApeKI GCWGC 3 cut(s) 68, 105, 606
ApoI RAATTY 1 cut(s) 290
Asp700I GAANNNNTTC 1 cut(s) 654
AspS9I GGNCC 1 cut(s) 298
AsuHPI GGTGA 3 cut(s) 134, 196, 325
AsuII TTCGAA 2 cut(s) 286, 505
BanI GGYRCC 1 cut(s) 875
BbsI GAAGAC 1 cut(s) 633
Bbv12I GWGCWC 1 cut(s) 805
BbvCI CCTCAGC 1 cut(s) 101
BbvI GCAGC 3 cut(s) 80, 92, 593
BccI CCATC 6 cut(s) 131, 299, 488, 578, 740, 866
BceAI ACGGC 1 cut(s) 165
BciVI GTATCC 1 cut(s) 806
BclI TGATCA 1 cut(s) 367
BcoDI GTCTC 4 cut(s) 13, 358, 402, 632
BfmI CTRYAG 1 cut(s) 391
BfuAI ACCTGC 1 cut(s) 384
BfuI GTATCC 1 cut(s) 806
BglII AGATCT 1 cut(s) 13
BisI GCNGC 3 cut(s) 69, 106, 607
BlsI GCNGC 3 cut(s) 70, 107, 608
BmcAI AGTACT 1 cut(s) 406
BmeRI GACNNNNNGTC 2 cut(s) 394, 624
BmgT120I GGNCC 1 cut(s) 298
BmiI GGNNCC 3 cut(s) 137, 299, 877
BmsI GCATC 5 cut(s) 159, 278, 709, 766, 788
BpiI GAAGAC 1 cut(s) 633
Bpu10I CCTNAGC 1 cut(s) 101
Bpu14I TTCGAA 2 cut(s) 286, 505
BsaI GGTCTC 1 cut(s) 402
BsaJI CCNNGG 3 cut(s) 145, 175, 207
BsaWI WCCGGW 1 cut(s) 272
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse3DI GCAATG 1 cut(s) 374
BseDI CCNNGG 3 cut(s) 145, 175, 207
BseGI GGATG 7 cut(s) 123, 150, 268, 269, 724, 751, 835
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMI GCAATG 1 cut(s) 374
BseMII CTCAG 6 cut(s) 24, 115, 369, 399, 462, 732
BseXI GCAGC 3 cut(s) 80, 92, 593
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 3 cut(s) 180, 300, 672
BshNI GGYRCC 1 cut(s) 875
BsiEI CGRYCG 1 cut(s) 277
BsiHKAI GWGCWC 1 cut(s) 805
BsiSI CCGG 1 cut(s) 273
BslFI GGGAC 3 cut(s) 157, 401, 564
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 4 cut(s) 13, 358, 402, 632
BsmBI CGTCTC 1 cut(s) 632
BsmFI GGGAC 3 cut(s) 157, 401, 564
BsmI GAATGC 1 cut(s) 322
BsnI GGCC 3 cut(s) 180, 300, 672
Bso31I GGTCTC 1 cut(s) 402
Bsp119I TTCGAA 2 cut(s) 286, 505
Bsp1286I GDGCHC 1 cut(s) 805
Bsp143I GATC 3 cut(s) 13, 367, 737
Bsp19I CCATGG 1 cut(s) 175
BspACI CCGC 1 cut(s) 108
BspANI GGCC 3 cut(s) 180, 300, 672
BspCNI CTCAG 6 cut(s) 23, 114, 368, 400, 463, 733
BspLI GGNNCC 3 cut(s) 137, 299, 877
BspMAI CTGCAG 1 cut(s) 395
BspMI ACCTGC 1 cut(s) 384
BspPI GGATC 1 cut(s) 745
BspT104I TTCGAA 2 cut(s) 286, 505
BspT107I GGYRCC 1 cut(s) 875
BspTNI GGTCTC 1 cut(s) 402
BsrDI GCAATG 1 cut(s) 374
BssECI CCNNGG 3 cut(s) 145, 175, 207
BssMI GATC 3 cut(s) 13, 367, 737
BssT1I CCWWGG 1 cut(s) 175
Bst4CI ACNGT 5 cut(s) 146, 404, 502, 566, 701
Bst6I CTCTTC 1 cut(s) 523
BstBI TTCGAA 2 cut(s) 286, 505
BstC8I GCNNGC 2 cut(s) 381, 604
BstDEI CTNAG 7 cut(s) 10, 101, 355, 408, 471, 610, 741
BstDSI CCRYGG 2 cut(s) 145, 175
BstF5I GGATG 7 cut(s) 123, 150, 268, 269, 724, 751, 835
BstKTI GATC 3 cut(s) 16, 370, 740
BstMAI GTCTC 4 cut(s) 13, 358, 402, 632
BstMBI GATC 3 cut(s) 13, 367, 737
BstMCI CGRYCG 1 cut(s) 277
BstMWI GCNNNNNNNGC 2 cut(s) 65, 105
BstSFI CTRYAG 1 cut(s) 391
BstV1I GCAGC 3 cut(s) 80, 92, 593
BstV2I GAAGAC 1 cut(s) 633
BstX2I RGATCY 2 cut(s) 13, 737
BstYI RGATCY 2 cut(s) 13, 737
BsuI GTATCC 1 cut(s) 806
BsuRI GGCC 3 cut(s) 180, 300, 672
BtgI CCRYGG 2 cut(s) 145, 175
BtsCI GGATG 7 cut(s) 123, 150, 268, 269, 724, 751, 835
BtsIMutI CAGTG 1 cut(s) 819
BveI ACCTGC 1 cut(s) 384
Cac8I GCNNGC 2 cut(s) 381, 604
Cfr13I GGNCC 1 cut(s) 298
Csp6I GTAC 2 cut(s) 38, 405
CviAII CATG 6 cut(s) 50, 176, 200, 302, 725, 853
CviJI RGCY 6 cut(s) 105, 180, 300, 412, 606, 672
CviKI_1 RGCY 6 cut(s) 105, 180, 300, 412, 606, 672
CviQI GTAC 2 cut(s) 38, 405
DdeI CTNAG 7 cut(s) 10, 101, 355, 408, 471, 610, 741
DpnI GATC 3 cut(s) 15, 369, 739
DpnII GATC 3 cut(s) 13, 367, 737
DrdI GACNNNNNNGTC 1 cut(s) 169
DriI GACNNNNNGTC 2 cut(s) 394, 624
DseDI GACNNNNNNGTC 1 cut(s) 169
EaeI YGGCCR 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 523
Eam1105I GACNNNNNGTC 2 cut(s) 394, 624
EarI CTCTTC 1 cut(s) 523
Eco130I CCWWGG 1 cut(s) 175
Eco31I GGTCTC 1 cut(s) 402
EcoT14I CCWWGG 1 cut(s) 175
EcoT22I ATGCAT 1 cut(s) 781
ErhI CCWWGG 1 cut(s) 175
Esp3I CGTCTC 1 cut(s) 632
FaeI CATG 6 cut(s) 53, 179, 203, 305, 728, 856
FaqI GGGAC 3 cut(s) 157, 401, 564
FatI CATG 6 cut(s) 49, 175, 199, 301, 724, 852
FauI CCCGC 1 cut(s) 101
FbaI TGATCA 1 cut(s) 367
Fnu4HI GCNGC 3 cut(s) 69, 106, 607
FokI GGATG 7 cut(s) 110, 137, 256, 275, 731, 758, 822
Fsp4HI GCNGC 3 cut(s) 69, 106, 607
GluI GCNGC 3 cut(s) 69, 106, 607
HaeIII GGCC 3 cut(s) 180, 300, 672
HapII CCGG 1 cut(s) 273
Hin1II CATG 6 cut(s) 53, 179, 203, 305, 728, 856
HinfI GANTC 4 cut(s) 129, 279, 428, 614
HpaII CCGG 1 cut(s) 273
HphI GGTGA 3 cut(s) 134, 196, 325
Hpy166II GTNNAC 1 cut(s) 562
Hpy188I TCNGA 8 cut(s) 13, 128, 211, 420, 541, 734, 742, 800
Hpy188III TCNNGA 2 cut(s) 133, 659
Hpy8I GTNNAC 1 cut(s) 562
HpyAV CCTTC 1 cut(s) 19
HpyCH4III ACNGT 5 cut(s) 146, 404, 502, 566, 701
HpyCH4IV ACGT 1 cut(s) 625
HpyCH4V TGCA 5 cut(s) 269, 393, 594, 779, 852
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 105
HpyF3I CTNAG 7 cut(s) 10, 101, 355, 408, 471, 610, 741
HpySE526I ACGT 1 cut(s) 625
Hsp92II CATG 6 cut(s) 53, 179, 203, 305, 728, 856
Ksp22I TGATCA 1 cut(s) 367
Kzo9I GATC 3 cut(s) 13, 367, 737
LmnI GCTCC 1 cut(s) 56
LpnPI CCDG 8 cut(s) 118, 216, 286, 345, 379, 570, 622, 748
Lsp1109I GCAGC 3 cut(s) 80, 92, 593
LweI GCATC 5 cut(s) 159, 278, 709, 766, 788
MaeII ACGT 1 cut(s) 625
MaeIII GTNAC 3 cut(s) 202, 587, 817
MalI GATC 3 cut(s) 15, 369, 739
MboI GATC 3 cut(s) 13, 367, 737
MboII GAAGA 4 cut(s) 244, 540, 633, 751
MflI RGATCY 2 cut(s) 13, 737
MhlI GDGCHC 1 cut(s) 805
MluCI AATT 8 cut(s) 91, 221, 290, 423, 477, 650, 692, 753
MlyI GAGTC 3 cut(s) 123, 288, 422
MmeI TCCRAC 1 cut(s) 151
MnlI CCTC 9 cut(s) 22, 46, 110, 150, 217, 302, 728, 736, 874
Mph1103I ATGCAT 1 cut(s) 781
MroXI GAANNNNTTC 1 cut(s) 654
MseI TTAA 4 cut(s) 42, 159, 461, 882
MslI CAYNNNNRTG 3 cut(s) 180, 521, 884
MspA1I CMGCKG 1 cut(s) 105
MspI CCGG 1 cut(s) 273
Mva1269I GAATGC 1 cut(s) 322
MwoI GCNNNNNNNGC 2 cut(s) 65, 105
NcoI CCATGG 1 cut(s) 175
NdeII GATC 3 cut(s) 13, 367, 737
NlaIII CATG 6 cut(s) 53, 179, 203, 305, 728, 856
NlaIV GGNNCC 3 cut(s) 137, 299, 877
NmuCI GTSAC 1 cut(s) 202
NsiI ATGCAT 1 cut(s) 781
NspV TTCGAA 2 cut(s) 286, 505
PcsI WCGNNNNNNNCGW 1 cut(s) 283
PctI GAATGC 1 cut(s) 322
PdmI GAANNNNTTC 1 cut(s) 654
PfeI GAWTC 1 cut(s) 614
PkrI GCNGC 3 cut(s) 70, 107, 608
PleI GAGTC 3 cut(s) 123, 287, 422
PpsI GAGTC 3 cut(s) 123, 287, 422
PspN4I GGNNCC 3 cut(s) 137, 299, 877
PspPI GGNCC 1 cut(s) 298
PstI CTGCAG 1 cut(s) 395
PsuI RGATCY 2 cut(s) 13, 737
PvuII CAGCTG 1 cut(s) 105
RsaI GTAC 2 cut(s) 39, 406
RsaNI GTAC 2 cut(s) 38, 405
RseI CAYNNNNRTG 3 cut(s) 180, 521, 884
SaqAI TTAA 4 cut(s) 42, 159, 461, 882
SatI GCNGC 3 cut(s) 69, 106, 607
Sau3AI GATC 3 cut(s) 13, 367, 737
Sau96I GGNCC 1 cut(s) 298
ScaI AGTACT 1 cut(s) 406
SchI GAGTC 3 cut(s) 123, 288, 422
SduI GDGCHC 1 cut(s) 805
SfaNI GCATC 5 cut(s) 159, 278, 709, 766, 788
SfcI CTRYAG 1 cut(s) 391
SfuI TTCGAA 2 cut(s) 286, 505
SmiMI CAYNNNNRTG 3 cut(s) 180, 521, 884
Sse9I AATT 8 cut(s) 91, 221, 290, 423, 477, 650, 692, 753
SsiI CCGC 1 cut(s) 108
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 5 cut(s) 146, 404, 502, 566, 701
TaiI ACGT 1 cut(s) 628
TaqI TCGA 4 cut(s) 277, 286, 505, 617
TaqII GACCGA 1 cut(s) 132
TasI AATT 8 cut(s) 91, 221, 290, 423, 477, 650, 692, 753
TatI WGTACW 2 cut(s) 37, 404
TfiI GAWTC 1 cut(s) 614
Tru1I TTAA 4 cut(s) 42, 159, 461, 882
Tru9I TTAA 4 cut(s) 42, 159, 461, 882
TscAI CASTG 1 cut(s) 819
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 3 cut(s) 68, 105, 606
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 1 cut(s) 798
TspGWI ACGGA 3 cut(s) 102, 179, 731
TspRI CASTG 1 cut(s) 819
XapI RAATTY 1 cut(s) 290
XmnI GAANNNNTTC 1 cut(s) 654
ZrmI AGTACT 1 cut(s) 406
Zsp2I ATGCAT 1 cut(s) 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.