Rw2G022500

WD repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005405
Physical Location & Seq
Forward (+)
32030931 .. 32037689
6759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G022500.1

Sequence Viewer

Length: 900 bp
ATGAGTGTCTCAGATCTGCCAAAGAAGGAGGTAAATGTACTTAAAGGGCATGAGGGAGCAGTATTAGCAGCGAGGTTCAATAAAGACGGAAATTACTGCCTCAGCTGCGGGAAGGACCGAACCATACGACTCTGGAACCCTCACCGTGGCATCCACATTAAGACCTACAAGTCCCATGGCCGTGAAGTCCGTGATGTCCATGTCACCTTGGACAACTCCAAATTATGTTCAGGTGGTGGCGACAGACAAATCTTCTATTGGGATGTGGCATCCGGTCGAGTCATTCGAAAATTTCGGGGCCATGATGGAGGGGTGAATGCTGTGAAGTTCAATGAGTATGCATCTGTTGTAGTCTCAGCAGGATATGATCAGTCATTGCGTGCTTGGGACTGCAGGTCTCAGAGTACTGAGCCTATTCAGATAATTGACTCGTTTACAGATAGTGTAATGTCTGTTTGTTTAACAAAGACTGAGATAATTGGTGGAAGTGTTGATGGAACTGTTCGAACATTTGACATTCGTATGGGAAGAGAGCTATCCGATGACTTGGGACAACCTGTGAACTGTATCTCAATGTCAAATGATGGTAACTGCATATTAGCAAGCTGCTTAGATTCGACTATACGACTCCTCGACAGGTCTTCCGCTGAATTACTTCAAGAATACAAAGGCCATACTTGTAAGTCTTACAAATTGGACTGCTGCCTTACCAACACTGATGCCCATGTAATCGGAGGATCTGAGGATGGTTCAATTTTCTTCTGGGATCTAGTAGATGCATCTGTTGTGTCAAGTATCCGAGCACATTCGTCAGTGGTTACAAGTGTCAGTTATCACCCAAAAGACAACTGCATGGTTAGTTCTTCTGTAGATGGCACTATTAAGGTGTGGAAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

299

Amino Acids

32.87

Weight (kDa)

6.78

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WDR55 PF24796 8 - 181 2.1e-14 WDR55
WD40 PF00400 9 - 46 8.9e-10 WD domain, G-beta repeat
WD40_Prp19 PF24814 9 - 68 3.7e-07 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 9 - 97 2.4e-13 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 10 - 71 2.8e-09 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 10 - 97 3.4e-09 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 10 - 131 4.3e-13 TEP-1 second beta-propeller
Beta-prop_EML PF23409 10 - 157 2.3e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40_Gbeta PF25391 13 - 154 1.9e-12 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 13 - 130 2.7e-15 WDR3 second beta-propeller domain
EIF3I PF24805 13 - 225 3.1e-24 EIF3I
Beta-prop_WDR5 PF25175 14 - 298 1e-65 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 16 - 147 1.8e-11 WDR36/Utp21 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 28 - 129 1.1e-07 MABP1/WDR62 second WD40 domain
Beta-prop_SCAP PF24017 37 - 133 8.5e-07 SCAP Beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 40 - 129 4.7e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 41 - 173 1.9e-21 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 52 - 135 9.2e-07 CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz PF24807 53 - 135 9.3e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR41 PF25178 53 - 155 7.6e-07 WDR41
Beta-prop_THOC3 PF25174 59 - 299 5.9e-32 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 62 - 137 3.9e-08 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 62 - 172 4.9e-10 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 71 - 196 2.5e-16 Prp19 WD40 domain
WD40 PF00400 93 - 130 1.6e-06 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 97 - 184 6.8e-07 TEP-1 second beta-propeller
WD40_MABP1-WDR62_2nd PF24782 99 - 257 3.6e-07 MABP1/WDR62 second WD40 domain
Beta-prop_EML_2 PF23414 112 - 229 6.9e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 147 - 265 4.6e-08 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 155 - 292 4.6e-10 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 155 - 298 1.2e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 159 - 297 1.4e-12 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 184 - 298 6.5e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 185 - 298 1.9e-18 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 185 - 298 9.4e-10 TEP-1 second beta-propeller
WD40_Prp19 PF24814 187 - 297 9.2e-17 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 187 - 298 4.4e-12 WDR3 second beta-propeller domain
WDR55 PF24796 187 - 298 1.6e-11 WDR55
Beta-prop_ACSF4 PF13570 191 - 296 2.9e-07 Beta-alanine-activating enzyme, beta-propeller
WD40_MABP1-WDR62_2nd PF24782 192 - 298 2.9e-08 MABP1/WDR62 second WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 223 - 298 2.1e-06 CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st PF24817 235 - 298 2e-10 WDHD1 first WD40 domain
WD40 PF00400 262 - 297 3.3e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 169
Acc36I ACCTGC 1 cut(s) 384
AciI CCGC 2 cut(s) 108, 645
AclWI GGATC 2 cut(s) 745, 774
AcoI YGGCCR 1 cut(s) 178
AcsI RAATTY 1 cut(s) 290
AfaI GTAC 2 cut(s) 39, 406
AfiI CCNNNNNNNGG 1 cut(s) 146
AgsI TTSAA 4 cut(s) 79, 331, 659, 753
AhdI GACNNNNNGTC 1 cut(s) 394
AluBI AGCT 3 cut(s) 105, 535, 606
AluI AGCT 3 cut(s) 105, 535, 606
Alw21I GWGCWC 1 cut(s) 805
Alw26I GTCTC 3 cut(s) 13, 358, 402
AlwI GGATC 2 cut(s) 745, 774
AoxI GGCC 3 cut(s) 178, 298, 670
ApeKI GCWGC 4 cut(s) 68, 105, 606, 702
ApoI RAATTY 1 cut(s) 290
Asp700I GAANNNNTTC 1 cut(s) 654
AspS9I GGNCC 2 cut(s) 115, 298
AsuHPI GGTGA 4 cut(s) 134, 196, 325, 827
AsuII TTCGAA 2 cut(s) 286, 505
AvaII GGWCC 1 cut(s) 115
BbsI GAAGAC 1 cut(s) 633
Bbv12I GWGCWC 1 cut(s) 805
BbvCI CCTCAGC 1 cut(s) 101
BbvI GCAGC 4 cut(s) 80, 92, 593, 689
BccI CCATC 5 cut(s) 299, 488, 578, 740, 866
BceAI ACGGC 1 cut(s) 165
BciVI GTATCC 1 cut(s) 806
BclI TGATCA 1 cut(s) 367
BcoDI GTCTC 3 cut(s) 13, 358, 402
BfaI CTAG 1 cut(s) 770
BfmI CTRYAG 2 cut(s) 391, 867
BfuAI ACCTGC 1 cut(s) 384
BfuI GTATCC 1 cut(s) 806
BglII AGATCT 1 cut(s) 13
BisI GCNGC 4 cut(s) 69, 106, 607, 703
BlsI GCNGC 4 cut(s) 70, 107, 608, 704
BmcAI AGTACT 1 cut(s) 406
Bme18I GGWCC 1 cut(s) 115
BmeRI GACNNNNNGTC 1 cut(s) 394
BmgT120I GGNCC 2 cut(s) 115, 298
BmiI GGNNCC 2 cut(s) 137, 299
BmsI GCATC 6 cut(s) 159, 278, 350, 709, 766, 788
BpiI GAAGAC 1 cut(s) 633
Bpu10I CCTNAGC 1 cut(s) 101
Bpu14I TTCGAA 2 cut(s) 286, 505
BsaI GGTCTC 1 cut(s) 402
BsaJI CCNNGG 3 cut(s) 145, 175, 207
BsaWI WCCGGW 1 cut(s) 272
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse3DI GCAATG 1 cut(s) 374
BseDI CCNNGG 3 cut(s) 145, 175, 207
BseGI GGATG 4 cut(s) 150, 268, 269, 751
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMI GCAATG 1 cut(s) 374
BseMII CTCAG 7 cut(s) 24, 115, 369, 399, 413, 462, 732
BseRI GAGGAG 1 cut(s) 620
BseXI GCAGC 4 cut(s) 80, 92, 593, 689
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 3 cut(s) 180, 300, 672
BsiEI CGRYCG 1 cut(s) 277
BsiHKAI GWGCWC 1 cut(s) 805
BsiSI CCGG 1 cut(s) 273
BslFI GGGAC 3 cut(s) 157, 401, 564
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 3 cut(s) 13, 358, 402
BsmFI GGGAC 3 cut(s) 157, 401, 564
BsmI GAATGC 1 cut(s) 322
BsnI GGCC 3 cut(s) 180, 300, 672
Bso31I GGTCTC 1 cut(s) 402
Bsp119I TTCGAA 2 cut(s) 286, 505
Bsp1286I GDGCHC 1 cut(s) 805
Bsp143I GATC 4 cut(s) 13, 367, 737, 766
Bsp19I CCATGG 1 cut(s) 175
BspACI CCGC 2 cut(s) 108, 645
BspANI GGCC 3 cut(s) 180, 300, 672
BspCNI CTCAG 7 cut(s) 23, 114, 368, 400, 412, 463, 733
BspLI GGNNCC 2 cut(s) 137, 299
BspMAI CTGCAG 1 cut(s) 395
BspMI ACCTGC 1 cut(s) 384
BspPI GGATC 2 cut(s) 745, 774
BspT104I TTCGAA 2 cut(s) 286, 505
BspTNI GGTCTC 1 cut(s) 402
BsrDI GCAATG 1 cut(s) 374
BssECI CCNNGG 3 cut(s) 145, 175, 207
BssMI GATC 4 cut(s) 13, 367, 737, 766
BssT1I CCWWGG 2 cut(s) 175, 207
Bst4CI ACNGT 3 cut(s) 146, 502, 566
Bst6I CTCTTC 1 cut(s) 523
BstBI TTCGAA 2 cut(s) 286, 505
BstC8I GCNNGC 2 cut(s) 381, 604
BstDEI CTNAG 8 cut(s) 10, 101, 355, 399, 408, 471, 610, 741
BstDSI CCRYGG 2 cut(s) 145, 175
BstF5I GGATG 4 cut(s) 150, 268, 269, 751
BstKTI GATC 4 cut(s) 16, 370, 740, 769
BstMAI GTCTC 3 cut(s) 13, 358, 402
BstMBI GATC 4 cut(s) 13, 367, 737, 766
BstMCI CGRYCG 1 cut(s) 277
BstMWI GCNNNNNNNGC 2 cut(s) 65, 105
BstSFI CTRYAG 2 cut(s) 391, 867
BstV1I GCAGC 4 cut(s) 80, 92, 593, 689
BstV2I GAAGAC 1 cut(s) 633
BstX2I RGATCY 3 cut(s) 13, 737, 766
BstYI RGATCY 3 cut(s) 13, 737, 766
BsuI GTATCC 1 cut(s) 806
BsuRI GGCC 3 cut(s) 180, 300, 672
BtgI CCRYGG 2 cut(s) 145, 175
BtsCI GGATG 4 cut(s) 150, 268, 269, 751
BtsIMutI CAGTG 2 cut(s) 714, 819
BveI ACCTGC 1 cut(s) 384
Cac8I GCNNGC 2 cut(s) 381, 604
Cfr13I GGNCC 2 cut(s) 115, 298
Csp6I GTAC 2 cut(s) 38, 405
CviAII CATG 6 cut(s) 50, 176, 200, 302, 725, 853
CviJI RGCY 7 cut(s) 105, 180, 300, 412, 535, 606, 672
CviKI_1 RGCY 7 cut(s) 105, 180, 300, 412, 535, 606, 672
CviQI GTAC 2 cut(s) 38, 405
DdeI CTNAG 8 cut(s) 10, 101, 355, 399, 408, 471, 610, 741
DpnI GATC 4 cut(s) 15, 369, 739, 768
DpnII GATC 4 cut(s) 13, 367, 737, 766
DrdI GACNNNNNNGTC 1 cut(s) 169
DriI GACNNNNNGTC 1 cut(s) 394
DseDI GACNNNNNNGTC 1 cut(s) 169
EaeI YGGCCR 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 523
Eam1105I GACNNNNNGTC 1 cut(s) 394
EarI CTCTTC 1 cut(s) 523
Eco130I CCWWGG 2 cut(s) 175, 207
Eco31I GGTCTC 1 cut(s) 402
Eco47I GGWCC 1 cut(s) 115
EcoT14I CCWWGG 2 cut(s) 175, 207
EcoT22I ATGCAT 2 cut(s) 343, 781
ErhI CCWWGG 2 cut(s) 175, 207
FaeI CATG 6 cut(s) 53, 179, 203, 305, 728, 856
FaqI GGGAC 3 cut(s) 157, 401, 564
FatI CATG 6 cut(s) 49, 175, 199, 301, 724, 852
FauI CCCGC 1 cut(s) 101
FbaI TGATCA 1 cut(s) 367
Fnu4HI GCNGC 4 cut(s) 69, 106, 607, 703
FokI GGATG 4 cut(s) 137, 256, 275, 758
Fsp4HI GCNGC 4 cut(s) 69, 106, 607, 703
FspBI CTAG 1 cut(s) 770
GluI GCNGC 4 cut(s) 69, 106, 607, 703
HaeIII GGCC 3 cut(s) 180, 300, 672
HapII CCGG 1 cut(s) 273
Hin1II CATG 6 cut(s) 53, 179, 203, 305, 728, 856
HinfI GANTC 5 cut(s) 129, 279, 428, 614, 627
HpaII CCGG 1 cut(s) 273
HphI GGTGA 4 cut(s) 134, 196, 325, 827
Hpy166II GTNNAC 2 cut(s) 435, 562
Hpy188I TCNGA 7 cut(s) 13, 402, 420, 541, 734, 742, 800
Hpy188III TCNNGA 2 cut(s) 133, 659
Hpy8I GTNNAC 2 cut(s) 435, 562
HpyAV CCTTC 2 cut(s) 19, 106
HpyCH4III ACNGT 3 cut(s) 146, 502, 566
HpyCH4V TGCA 5 cut(s) 341, 393, 594, 779, 852
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 105
HpyF3I CTNAG 8 cut(s) 10, 101, 355, 399, 408, 471, 610, 741
Hsp92II CATG 6 cut(s) 53, 179, 203, 305, 728, 856
Ksp22I TGATCA 1 cut(s) 367
Kzo9I GATC 4 cut(s) 13, 367, 737, 766
LmnI GCTCC 1 cut(s) 56
LpnPI CCDG 8 cut(s) 118, 216, 286, 345, 379, 570, 622, 748
Lsp1109I GCAGC 4 cut(s) 80, 92, 593, 689
LweI GCATC 6 cut(s) 159, 278, 350, 709, 766, 788
MaeI CTAG 1 cut(s) 770
MaeIII GTNAC 3 cut(s) 202, 587, 817
MalI GATC 4 cut(s) 15, 369, 739, 768
MboI GATC 4 cut(s) 13, 367, 737, 766
MboII GAAGA 5 cut(s) 244, 540, 633, 751, 855
MflI RGATCY 3 cut(s) 13, 737, 766
MhlI GDGCHC 1 cut(s) 805
MluCI AATT 8 cut(s) 91, 221, 290, 423, 477, 650, 692, 753
MlyI GAGTC 4 cut(s) 123, 288, 422, 621
MnlI CCTC 9 cut(s) 22, 46, 66, 110, 150, 302, 641, 728, 736
Mph1103I ATGCAT 2 cut(s) 343, 781
MroXI GAANNNNTTC 1 cut(s) 654
MseI TTAA 4 cut(s) 42, 159, 461, 882
MslI CAYNNNNRTG 2 cut(s) 180, 521
MspA1I CMGCKG 2 cut(s) 105, 647
MspI CCGG 1 cut(s) 273
Mva1269I GAATGC 1 cut(s) 322
MwoI GCNNNNNNNGC 2 cut(s) 65, 105
NcoI CCATGG 1 cut(s) 175
NdeII GATC 4 cut(s) 13, 367, 737, 766
NlaIII CATG 6 cut(s) 53, 179, 203, 305, 728, 856
NlaIV GGNNCC 2 cut(s) 137, 299
NmuCI GTSAC 1 cut(s) 202
NsiI ATGCAT 2 cut(s) 343, 781
NspV TTCGAA 2 cut(s) 286, 505
PcsI WCGNNNNNNNCGW 1 cut(s) 283
PctI GAATGC 1 cut(s) 322
PdmI GAANNNNTTC 1 cut(s) 654
PfeI GAWTC 1 cut(s) 614
PkrI GCNGC 4 cut(s) 70, 107, 608, 704
PleI GAGTC 4 cut(s) 123, 287, 422, 621
PpsI GAGTC 4 cut(s) 123, 287, 422, 621
PspN4I GGNNCC 2 cut(s) 137, 299
PspPI GGNCC 2 cut(s) 115, 298
PstI CTGCAG 1 cut(s) 395
PsuI RGATCY 3 cut(s) 13, 737, 766
PvuII CAGCTG 1 cut(s) 105
RsaI GTAC 2 cut(s) 39, 406
RsaNI GTAC 2 cut(s) 38, 405
RseI CAYNNNNRTG 2 cut(s) 180, 521
SaqAI TTAA 4 cut(s) 42, 159, 461, 882
SatI GCNGC 4 cut(s) 69, 106, 607, 703
Sau3AI GATC 4 cut(s) 13, 367, 737, 766
Sau96I GGNCC 2 cut(s) 115, 298
ScaI AGTACT 1 cut(s) 406
SchI GAGTC 4 cut(s) 123, 288, 422, 621
SduI GDGCHC 1 cut(s) 805
SfaNI GCATC 6 cut(s) 159, 278, 350, 709, 766, 788
SfcI CTRYAG 2 cut(s) 391, 867
SfuI TTCGAA 2 cut(s) 286, 505
SinI GGWCC 1 cut(s) 115
SmiMI CAYNNNNRTG 2 cut(s) 180, 521
Sse9I AATT 8 cut(s) 91, 221, 290, 423, 477, 650, 692, 753
SsiI CCGC 2 cut(s) 108, 645
SspMI CTAG 1 cut(s) 770
StyI CCWWGG 2 cut(s) 175, 207
TaaI ACNGT 3 cut(s) 146, 502, 566
TaqI TCGA 5 cut(s) 277, 286, 505, 617, 633
TaqII GACCGA 1 cut(s) 132
TasI AATT 8 cut(s) 91, 221, 290, 423, 477, 650, 692, 753
TatI WGTACW 2 cut(s) 37, 404
TfiI GAWTC 1 cut(s) 614
Tru1I TTAA 4 cut(s) 42, 159, 461, 882
Tru9I TTAA 4 cut(s) 42, 159, 461, 882
TscAI CASTG 2 cut(s) 721, 819
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 4 cut(s) 68, 105, 606, 702
Tsp45I GTSAC 1 cut(s) 202
TspGWI ACGGA 2 cut(s) 102, 179
TspRI CASTG 2 cut(s) 721, 819
VpaK11BI GGWCC 1 cut(s) 115
XapI RAATTY 1 cut(s) 290
XmnI GAANNNNTTC 1 cut(s) 654
XspI CTAG 1 cut(s) 770
ZrmI AGTACT 1 cut(s) 406
Zsp2I ATGCAT 2 cut(s) 343, 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.