Rh6BG094400

WD repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0005405
Physical Location & Seq
Reverse (-)
14879711 .. 14886613
6903 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG094400.1

Sequence Viewer

Length: 786 bp
ATGAGTGTCTCAGATCTGCCAAAGAAGGAGGTAAATGTACTTAAAGGGCATGAGGGAGCAGTATTAGCAGCGAGGTTCAATAAAGACGGAAATTACTGCCTCAGCTGCGGGAAAGACCGAACCATACGACTCTGGAACCCTCACCGTGGCATCCACATTAAGACCTACAAGTCCCATGGCCGTGAAGTCCGTGATGTCCATGTCACCTTGGACAACTCCAAATTATGTTCAGGTGGTGGCGACAGACAAATCTTCTATTGGGATGTGGCATCTGGTCGAGTCATTCGAAAATTTCGGGGCCATGATGGAGGGGTGAATGCTGTGAAGTTCAATGAGTATGCATCTGTTGTAGTCTCAGCAGGATATGATCAGTCATTGCGTGCTTGGGACTGCAGGTCTCAGAGTACTGAGCCTATTCAGATAATTGACTCGTTTACAGATAGTGTAATGTCTGTTTGTTTAACAAAGACTGAGATAATTGGTGGAAGTGTTGATGGTACTGTTCGAACATTTGACATTCGTATGGGAAGAGAGCTATCCGATGACTTGGGACAACCTGTGAACTGTATCTCAATGTCAAATGATGGTAACTGCATATTAGCAAGCTGCTTAGATTCGACTATACGACTCCTCGACAGGTCTTCTGCTGAATTACTTCAAGAATATAAAGGCCATACTTGTAAGGTTAGTATCTTATCTGGCTTCTGCCACTCTCTCATGGTTTCTTTGCCATTATCTAACTCGGATGCTCATTCCTCTTTTGTTTCTTTTGATATGTTGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

261

Amino Acids

28.76

Weight (kDa)

7.09

Isoelectric Point (pI)

45.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WDR55 PF24796 8 - 181 1.3e-14 WDR55
WD40 PF00400 9 - 46 7.3e-10 WD domain, G-beta repeat
WD40_Prp19 PF24814 9 - 68 2.7e-07 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 10 - 71 2.6e-09 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 10 - 99 1e-13 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 10 - 133 1.1e-13 TEP-1 second beta-propeller
Beta-prop_EML PF23409 11 - 158 1.6e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 13 - 130 2e-15 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 13 - 150 1.7e-12 G protein beta WD-40 repeat protein
EIF3I PF24805 13 - 226 1.5e-24 EIF3I
Beta-prop_WDR5 PF25175 14 - 236 7.2e-46 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 16 - 147 1.3e-11 WDR36/Utp21 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 28 - 129 8e-08 MABP1/WDR62 second WD40 domain
Beta-prop_SCAP PF24017 38 - 133 7.2e-07 SCAP Beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 41 - 129 5.7e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 41 - 173 1.3e-21 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 51 - 135 2.8e-07 CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz PF24807 53 - 135 6.4e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR41 PF25178 54 - 155 8.1e-07 WDR41
Beta-prop_THOC3 PF25174 59 - 231 8.3e-21 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 62 - 137 3e-08 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 62 - 172 3.6e-10 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 71 - 233 5.2e-22 Prp19 WD40 domain
WD40 PF00400 93 - 130 1.3e-06 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 97 - 219 1e-08 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 105 - 233 4.2e-09 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 112 - 230 2.7e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 169
Acc36I ACCTGC 1 cut(s) 384
AciI CCGC 1 cut(s) 108
AcoI YGGCCR 1 cut(s) 178
AcsI RAATTY 1 cut(s) 290
AfaI GTAC 3 cut(s) 39, 406, 499
AfiI CCNNNNNNNGG 1 cut(s) 146
AgsI TTSAA 3 cut(s) 79, 331, 659
AhdI GACNNNNNGTC 1 cut(s) 394
AluBI AGCT 3 cut(s) 105, 535, 606
AluI AGCT 3 cut(s) 105, 535, 606
Alw26I GTCTC 3 cut(s) 13, 358, 402
AoxI GGCC 3 cut(s) 178, 298, 670
ApeKI GCWGC 3 cut(s) 68, 105, 606
ApoI RAATTY 1 cut(s) 290
Asp700I GAANNNNTTC 1 cut(s) 654
AspS9I GGNCC 1 cut(s) 298
AsuHPI GGTGA 3 cut(s) 134, 196, 325
AsuII TTCGAA 2 cut(s) 286, 505
BbsI GAAGAC 1 cut(s) 633
BbvCI CCTCAGC 1 cut(s) 101
BbvI GCAGC 3 cut(s) 80, 92, 593
BccI CCATC 3 cut(s) 299, 488, 578
BceAI ACGGC 1 cut(s) 165
BclI TGATCA 1 cut(s) 367
BcoDI GTCTC 3 cut(s) 13, 358, 402
BfmI CTRYAG 1 cut(s) 391
BfuAI ACCTGC 1 cut(s) 384
BglII AGATCT 1 cut(s) 13
BisI GCNGC 3 cut(s) 69, 106, 607
BlsI GCNGC 3 cut(s) 70, 107, 608
BmcAI AGTACT 1 cut(s) 406
BmeRI GACNNNNNGTC 1 cut(s) 394
BmgT120I GGNCC 1 cut(s) 298
BmiI GGNNCC 2 cut(s) 137, 299
BmsI GCATC 4 cut(s) 159, 278, 350, 736
BpiI GAAGAC 1 cut(s) 633
Bpu10I CCTNAGC 1 cut(s) 101
Bpu14I TTCGAA 2 cut(s) 286, 505
BsaI GGTCTC 1 cut(s) 402
BsaJI CCNNGG 3 cut(s) 145, 175, 207
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse3DI GCAATG 1 cut(s) 374
BseDI CCNNGG 3 cut(s) 145, 175, 207
BseGI GGATG 3 cut(s) 150, 268, 751
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMI GCAATG 1 cut(s) 374
BseMII CTCAG 6 cut(s) 24, 115, 369, 399, 413, 462
BseRI GAGGAG 1 cut(s) 620
BseXI GCAGC 3 cut(s) 80, 92, 593
BshFI GGCC 3 cut(s) 180, 300, 672
BslFI GGGAC 3 cut(s) 157, 401, 564
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 3 cut(s) 13, 358, 402
BsmFI GGGAC 3 cut(s) 157, 401, 564
BsmI GAATGC 1 cut(s) 322
BsnI GGCC 3 cut(s) 180, 300, 672
Bso31I GGTCTC 1 cut(s) 402
Bsp119I TTCGAA 2 cut(s) 286, 505
Bsp143I GATC 2 cut(s) 13, 367
Bsp19I CCATGG 1 cut(s) 175
BspACI CCGC 1 cut(s) 108
BspANI GGCC 3 cut(s) 180, 300, 672
BspCNI CTCAG 6 cut(s) 23, 114, 368, 400, 412, 463
BspLI GGNNCC 2 cut(s) 137, 299
BspMAI CTGCAG 1 cut(s) 395
BspMI ACCTGC 1 cut(s) 384
BspT104I TTCGAA 2 cut(s) 286, 505
BspTNI GGTCTC 1 cut(s) 402
BsrDI GCAATG 1 cut(s) 374
BssECI CCNNGG 3 cut(s) 145, 175, 207
BssMI GATC 2 cut(s) 13, 367
BssT1I CCWWGG 2 cut(s) 175, 207
Bst4CI ACNGT 3 cut(s) 146, 502, 566
Bst6I CTCTTC 1 cut(s) 523
BstBI TTCGAA 2 cut(s) 286, 505
BstC8I GCNNGC 2 cut(s) 381, 604
BstDEI CTNAG 7 cut(s) 10, 101, 355, 399, 408, 471, 610
BstDSI CCRYGG 2 cut(s) 145, 175
BstF5I GGATG 3 cut(s) 150, 268, 751
BstKTI GATC 2 cut(s) 16, 370
BstMAI GTCTC 3 cut(s) 13, 358, 402
BstMBI GATC 2 cut(s) 13, 367
BstMWI GCNNNNNNNGC 2 cut(s) 65, 105
BstSFI CTRYAG 1 cut(s) 391
BstV1I GCAGC 3 cut(s) 80, 92, 593
BstV2I GAAGAC 1 cut(s) 633
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
BsuRI GGCC 3 cut(s) 180, 300, 672
BtgI CCRYGG 2 cut(s) 145, 175
BtsCI GGATG 3 cut(s) 150, 268, 751
BveI ACCTGC 1 cut(s) 384
Cac8I GCNNGC 2 cut(s) 381, 604
Cfr13I GGNCC 1 cut(s) 298
Csp6I GTAC 3 cut(s) 38, 405, 498
CviAII CATG 5 cut(s) 50, 176, 200, 302, 718
CviJI RGCY 8 cut(s) 105, 180, 300, 412, 535, 606, 672, 702
CviKI_1 RGCY 8 cut(s) 105, 180, 300, 412, 535, 606, 672, 702
CviQI GTAC 3 cut(s) 38, 405, 498
DdeI CTNAG 7 cut(s) 10, 101, 355, 399, 408, 471, 610
DpnI GATC 2 cut(s) 15, 369
DpnII GATC 2 cut(s) 13, 367
DrdI GACNNNNNNGTC 1 cut(s) 169
DriI GACNNNNNGTC 1 cut(s) 394
DseDI GACNNNNNNGTC 1 cut(s) 169
EaeI YGGCCR 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 523
Eam1105I GACNNNNNGTC 1 cut(s) 394
EarI CTCTTC 1 cut(s) 523
Eco130I CCWWGG 2 cut(s) 175, 207
Eco31I GGTCTC 1 cut(s) 402
EcoT14I CCWWGG 2 cut(s) 175, 207
EcoT22I ATGCAT 1 cut(s) 343
ErhI CCWWGG 2 cut(s) 175, 207
FaeI CATG 5 cut(s) 53, 179, 203, 305, 721
FaqI GGGAC 3 cut(s) 157, 401, 564
FatI CATG 5 cut(s) 49, 175, 199, 301, 717
FauI CCCGC 1 cut(s) 101
FbaI TGATCA 1 cut(s) 367
Fnu4HI GCNGC 3 cut(s) 69, 106, 607
FokI GGATG 3 cut(s) 137, 275, 758
Fsp4HI GCNGC 3 cut(s) 69, 106, 607
GluI GCNGC 3 cut(s) 69, 106, 607
HaeIII GGCC 3 cut(s) 180, 300, 672
Hin1II CATG 5 cut(s) 53, 179, 203, 305, 721
HinfI GANTC 5 cut(s) 129, 279, 428, 614, 627
HphI GGTGA 3 cut(s) 134, 196, 325
Hpy166II GTNNAC 2 cut(s) 435, 562
Hpy188I TCNGA 5 cut(s) 13, 402, 420, 541, 745
Hpy188III TCNNGA 2 cut(s) 133, 659
Hpy8I GTNNAC 2 cut(s) 435, 562
HpyAV CCTTC 1 cut(s) 19
HpyCH4III ACNGT 3 cut(s) 146, 502, 566
HpyCH4V TGCA 3 cut(s) 341, 393, 594
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 105
HpyF3I CTNAG 7 cut(s) 10, 101, 355, 399, 408, 471, 610
Hsp92II CATG 5 cut(s) 53, 179, 203, 305, 721
Ksp22I TGATCA 1 cut(s) 367
Kzo9I GATC 2 cut(s) 13, 367
LmnI GCTCC 1 cut(s) 56
LpnPI CCDG 8 cut(s) 118, 216, 258, 345, 379, 570, 622, 684
Lsp1109I GCAGC 3 cut(s) 80, 92, 593
LweI GCATC 4 cut(s) 159, 278, 350, 736
MaeIII GTNAC 2 cut(s) 202, 587
MalI GATC 2 cut(s) 15, 369
MboI GATC 2 cut(s) 13, 367
MboII GAAGA 3 cut(s) 244, 540, 633
MflI RGATCY 1 cut(s) 13
MluCI AATT 6 cut(s) 91, 221, 290, 423, 477, 650
MlyI GAGTC 4 cut(s) 123, 288, 422, 621
MnlI CCTC 8 cut(s) 22, 46, 66, 110, 150, 302, 641, 766
Mph1103I ATGCAT 1 cut(s) 343
MroXI GAANNNNTTC 1 cut(s) 654
MseI TTAA 3 cut(s) 42, 159, 461
MslI CAYNNNNRTG 2 cut(s) 180, 521
MspA1I CMGCKG 1 cut(s) 105
Mva1269I GAATGC 1 cut(s) 322
MwoI GCNNNNNNNGC 2 cut(s) 65, 105
NcoI CCATGG 1 cut(s) 175
NdeII GATC 2 cut(s) 13, 367
NlaIII CATG 5 cut(s) 53, 179, 203, 305, 721
NlaIV GGNNCC 2 cut(s) 137, 299
NmuCI GTSAC 1 cut(s) 202
NsiI ATGCAT 1 cut(s) 343
NspV TTCGAA 2 cut(s) 286, 505
PcsI WCGNNNNNNNCGW 1 cut(s) 283
PctI GAATGC 1 cut(s) 322
PdmI GAANNNNTTC 1 cut(s) 654
PfeI GAWTC 1 cut(s) 614
PkrI GCNGC 3 cut(s) 70, 107, 608
PleI GAGTC 4 cut(s) 123, 287, 422, 621
PpsI GAGTC 4 cut(s) 123, 287, 422, 621
PspN4I GGNNCC 2 cut(s) 137, 299
PspPI GGNCC 1 cut(s) 298
PstI CTGCAG 1 cut(s) 395
PsuI RGATCY 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 105
RsaI GTAC 3 cut(s) 39, 406, 499
RsaNI GTAC 3 cut(s) 38, 405, 498
RseI CAYNNNNRTG 2 cut(s) 180, 521
SaqAI TTAA 3 cut(s) 42, 159, 461
SatI GCNGC 3 cut(s) 69, 106, 607
Sau3AI GATC 2 cut(s) 13, 367
Sau96I GGNCC 1 cut(s) 298
ScaI AGTACT 1 cut(s) 406
SchI GAGTC 4 cut(s) 123, 288, 422, 621
SfaNI GCATC 4 cut(s) 159, 278, 350, 736
SfcI CTRYAG 1 cut(s) 391
SfuI TTCGAA 2 cut(s) 286, 505
SmiMI CAYNNNNRTG 2 cut(s) 180, 521
Sse9I AATT 6 cut(s) 91, 221, 290, 423, 477, 650
SsiI CCGC 1 cut(s) 108
StyI CCWWGG 2 cut(s) 175, 207
TaaI ACNGT 3 cut(s) 146, 502, 566
TaqI TCGA 5 cut(s) 277, 286, 505, 617, 633
TaqII GACCGA 1 cut(s) 132
TasI AATT 6 cut(s) 91, 221, 290, 423, 477, 650
TatI WGTACW 2 cut(s) 37, 404
TfiI GAWTC 1 cut(s) 614
Tru1I TTAA 3 cut(s) 42, 159, 461
Tru9I TTAA 3 cut(s) 42, 159, 461
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 3 cut(s) 68, 105, 606
Tsp45I GTSAC 1 cut(s) 202
TspGWI ACGGA 2 cut(s) 102, 179
XapI RAATTY 1 cut(s) 290
XmnI GAANNNNTTC 1 cut(s) 654
ZrmI AGTACT 1 cut(s) 406
Zsp2I ATGCAT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.