Rh6DG085600

WD repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0005405
Physical Location & Seq
Forward (+)
10012953 .. 10013937
985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG085600.1

Sequence Viewer

Length: 486 bp
ATGTCTGTTTGTTTAACAAAGACTGAGATAATTGGTGGAAGTGTTGATGGTACTGTTCGAACATTTGACATTCGTATGGGAAGAGAGCTATCCGATGACTTGGGACAACCTGTGAACTGTATCTCAATGTCAAATGATGGTAACTGCATATTAGCAAGCTGCTTAGATTCGACTATACGACTCCTCGACAGGTCTTCCGCTGAATTACTTCAAGAATATAAAGGCCATACTTGTAAGTCTTACAAATTGGACTGCTGCCTTACCAACACTGATGCCCATGTAATCGGAGGATCTGAGGATGGTTCAATTTTCTTCTGGGATCTAGTAGATGCATCTGTTGTGTCAAGTATCCGAGCACATTCGTCAGTGGTAAAGCCTATTTTTCATTTACATGAATTGTGGTTTTCCTTTCGAATAGTTCTGACTGCTCGTGATATCCCTGCTACTTTGTGTTCCCAAGTATCCCCAAATAAAATACAGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

161

Amino Acids

17.69

Weight (kDa)

5.28

Isoelectric Point (pI)

42.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 2 - 124 8.9e-09 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 6 - 129 1.1e-10 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 7 - 125 1.8e-23 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 7 - 123 2.5e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_ACSF4 PF13570 7 - 128 6.8e-07 Beta-alanine-activating enzyme, beta-propeller
Beta-prop_EML_2 PF23414 7 - 125 2.6e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3 PF25174 10 - 125 6.8e-11 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 10 - 127 1.7e-06 WDR36/Utp21 first beta-propeller
WDR55 PF24796 11 - 127 3.6e-07 WDR55
WD40_Gbeta PF25391 20 - 126 9.7e-06 G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd PF25047 24 - 132 7.8e-07 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 35 - 131 8.4e-10 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 38 - 126 2.1e-09 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 38 - 127 1.3e-06 WDR3 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 198
AclWI GGATC 2 cut(s) 298, 327
AfaI GTAC 1 cut(s) 52
AgsI TTSAA 2 cut(s) 212, 306
AluBI AGCT 2 cut(s) 88, 159
AluI AGCT 2 cut(s) 88, 159
Alw21I GWGCWC 1 cut(s) 358
AlwI GGATC 2 cut(s) 298, 327
AoxI GGCC 1 cut(s) 223
ApeKI GCWGC 2 cut(s) 159, 255
Asp700I GAANNNNTTC 1 cut(s) 207
AsuII TTCGAA 2 cut(s) 58, 412
BauI CACGAG 1 cut(s) 429
BbsI GAAGAC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 358
BbvI GCAGC 2 cut(s) 146, 242
BccI CCATC 3 cut(s) 41, 131, 293
BciVI GTATCC 2 cut(s) 359, 472
BfaI CTAG 1 cut(s) 323
BfuI GTATCC 2 cut(s) 359, 472
BisI GCNGC 2 cut(s) 160, 256
BlsI GCNGC 2 cut(s) 161, 257
BmsI GCATC 3 cut(s) 262, 319, 341
BpiI GAAGAC 1 cut(s) 186
Bpu14I TTCGAA 2 cut(s) 58, 412
BseGI GGATG 1 cut(s) 304
BseMII CTCAG 2 cut(s) 15, 285
BseRI GAGGAG 1 cut(s) 173
BseXI GCAGC 2 cut(s) 146, 242
BshFI GGCC 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 358
BslFI GGGAC 1 cut(s) 117
BsmFI GGGAC 1 cut(s) 117
BsnI GGCC 1 cut(s) 225
Bsp119I TTCGAA 2 cut(s) 58, 412
Bsp1286I GDGCHC 1 cut(s) 358
Bsp143I GATC 2 cut(s) 290, 319
BspACI CCGC 1 cut(s) 198
BspANI GGCC 1 cut(s) 225
BspCNI CTCAG 2 cut(s) 16, 286
BspPI GGATC 2 cut(s) 298, 327
BspT104I TTCGAA 2 cut(s) 58, 412
BssMI GATC 2 cut(s) 290, 319
BssSI CACGAG 1 cut(s) 429
Bst2BI CACGAG 1 cut(s) 429
Bst4CI ACNGT 2 cut(s) 55, 119
Bst6I CTCTTC 1 cut(s) 76
BstBI TTCGAA 2 cut(s) 58, 412
BstC8I GCNNGC 1 cut(s) 157
BstDEI CTNAG 3 cut(s) 24, 163, 294
BstF5I GGATG 1 cut(s) 304
BstKTI GATC 2 cut(s) 293, 322
BstMBI GATC 2 cut(s) 290, 319
BstV1I GCAGC 2 cut(s) 146, 242
BstV2I GAAGAC 1 cut(s) 186
BstX2I RGATCY 2 cut(s) 290, 319
BstYI RGATCY 2 cut(s) 290, 319
BsuI GTATCC 2 cut(s) 359, 472
BsuRI GGCC 1 cut(s) 225
BtsCI GGATG 1 cut(s) 304
BtsIMutI CAGTG 2 cut(s) 267, 372
Cac8I GCNNGC 1 cut(s) 157
Csp6I GTAC 1 cut(s) 51
CviAII CATG 2 cut(s) 278, 392
CviJI RGCY 4 cut(s) 88, 159, 225, 376
CviKI_1 RGCY 4 cut(s) 88, 159, 225, 376
CviQI GTAC 1 cut(s) 51
DdeI CTNAG 3 cut(s) 24, 163, 294
DpnI GATC 2 cut(s) 292, 321
DpnII GATC 2 cut(s) 290, 319
Eam1104I CTCTTC 1 cut(s) 76
EarI CTCTTC 1 cut(s) 76
Eco32I GATATC 1 cut(s) 436
EcoRV GATATC 1 cut(s) 436
EcoT22I ATGCAT 1 cut(s) 334
FaeI CATG 2 cut(s) 281, 395
FaiI YATR 7 cut(s) 77, 149, 176, 219, 228, 279, 393
FaqI GGGAC 1 cut(s) 117
FatI CATG 2 cut(s) 277, 391
Fnu4HI GCNGC 2 cut(s) 160, 256
FokI GGATG 1 cut(s) 311
Fsp4HI GCNGC 2 cut(s) 160, 256
FspBI CTAG 1 cut(s) 323
GluI GCNGC 2 cut(s) 160, 256
HaeIII GGCC 1 cut(s) 225
Hin1II CATG 2 cut(s) 281, 395
HinfI GANTC 2 cut(s) 167, 180
Hpy166II GTNNAC 1 cut(s) 115
Hpy188I TCNGA 5 cut(s) 94, 287, 295, 353, 423
Hpy188III TCNNGA 2 cut(s) 212, 431
Hpy8I GTNNAC 1 cut(s) 115
HpyCH4III ACNGT 2 cut(s) 55, 119
HpyCH4V TGCA 2 cut(s) 147, 332
HpyF3I CTNAG 3 cut(s) 24, 163, 294
Hsp92II CATG 2 cut(s) 281, 395
Kzo9I GATC 2 cut(s) 290, 319
LpnPI CCDG 5 cut(s) 123, 175, 301, 453, 464
Lsp1109I GCAGC 2 cut(s) 146, 242
LweI GCATC 3 cut(s) 262, 319, 341
MaeI CTAG 1 cut(s) 323
MaeIII GTNAC 1 cut(s) 140
MalI GATC 2 cut(s) 292, 321
MboI GATC 2 cut(s) 290, 319
MboII GAAGA 3 cut(s) 93, 186, 304
MflI RGATCY 2 cut(s) 290, 319
MhlI GDGCHC 1 cut(s) 358
MluCI AATT 5 cut(s) 30, 203, 245, 306, 395
MlyI GAGTC 1 cut(s) 174
MnlI CCTC 3 cut(s) 194, 281, 289
Mph1103I ATGCAT 1 cut(s) 334
MroXI GAANNNNTTC 1 cut(s) 207
MseI TTAA 1 cut(s) 14
MslI CAYNNNNRTG 2 cut(s) 74, 390
MspA1I CMGCKG 1 cut(s) 200
NdeII GATC 2 cut(s) 290, 319
NlaIII CATG 2 cut(s) 281, 395
NsiI ATGCAT 1 cut(s) 334
NspV TTCGAA 2 cut(s) 58, 412
PdmI GAANNNNTTC 1 cut(s) 207
PfeI GAWTC 1 cut(s) 167
PkrI GCNGC 2 cut(s) 161, 257
PleI GAGTC 1 cut(s) 174
PpsI GAGTC 1 cut(s) 174
PsuI RGATCY 2 cut(s) 290, 319
RsaI GTAC 1 cut(s) 52
RsaNI GTAC 1 cut(s) 51
RseI CAYNNNNRTG 2 cut(s) 74, 390
SaqAI TTAA 1 cut(s) 14
SatI GCNGC 2 cut(s) 160, 256
Sau3AI GATC 2 cut(s) 290, 319
SchI GAGTC 1 cut(s) 174
SduI GDGCHC 1 cut(s) 358
SetI ASST 4 cut(s) 90, 112, 161, 194
SfaNI GCATC 3 cut(s) 262, 319, 341
SfuI TTCGAA 2 cut(s) 58, 412
SmiMI CAYNNNNRTG 2 cut(s) 74, 390
Sse9I AATT 5 cut(s) 30, 203, 245, 306, 395
SsiI CCGC 1 cut(s) 198
SspMI CTAG 1 cut(s) 323
TaaI ACNGT 2 cut(s) 55, 119
TaqI TCGA 4 cut(s) 58, 170, 186, 412
TasI AATT 5 cut(s) 30, 203, 245, 306, 395
TfiI GAWTC 1 cut(s) 167
Tru1I TTAA 1 cut(s) 14
Tru9I TTAA 1 cut(s) 14
TscAI CASTG 2 cut(s) 274, 372
TseI GCWGC 2 cut(s) 159, 255
TspDTI ATGAA 2 cut(s) 374, 408
TspRI CASTG 2 cut(s) 274, 372
XmnI GAANNNNTTC 1 cut(s) 207
XspI CTAG 1 cut(s) 323
Zsp2I ATGCAT 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.