RchiOBHm_Chr2g0119791

WD repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005405
Physical Location & Seq
Forward (+)
32381287 .. 32383938
2652 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49247

Sequence Viewer

Length: 876 bp
ATGAGTGTCTCAGATCTGCCAAAGAAGGAGGTAAATGTACTTAAAGGGCATGAGGGAGCAGTATTAGCAGCGAGGTTCAATAAAGACGGAAATTACTGCCTCAGCTGCGGGAAAGACCGAACCATACGACTCTGGAACCCTCACCGTGGCATCCACATTAAGACCTACAAGTCCCATGGCCGTGAAGGACAACTCCAAATTATGTTCAGGTGGTGGCGACAGACAAATCTTCTATTGGGATGTGGCATCGGTCGAGTCATTCGAAAATTTCGGGGCCATGATGGAGGGGTGAATGCTGTGAAGTTCAATGAGTATGCATCTGTTGTAGTCTCAGCAGGATATGATCAGTCATTGCGTGCTTGGGACTGCAGGTCTCAGAGTACTGAGCCCATTCAGATAATTGACTCATTTACAGATAGTGTAATGTCTGTTTGTTTAACAAAGACTGAGATAATTGGTGGAAGTGTTGATGGAACTGTTCGAACATTTGACATTCGTATGGGAAGAGAGCTATCCGATGACTTGGGACAACCTGTGAACTGTATCTCAATGTCAAATGATGGTAACTGCATATTAGCAAGCTGCTTAGATTCGACTATACGACTCCTCGACAGGTCTTCTGCTGAATTACTTCAAGAATACAAAGGCCATACTTGTAAGTCTTACAAATTGGACTGCTGCCTTACCAACACTGATGCCCATGTAATCGGAGGATCTGAGGATGGTTCAATTTTCTTCTGGGATCTAGTAGATGCATCTGTTGTGTCAAGTATCCGAGCACATTCGTCAGTGGTTACAAGTGTCAGTTATCACCCGAAAGACAACTGCATGGTTAGTTCTTCTGTAGATGGCACTATTAAGGTGTGGAAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

291

Amino Acids

32.17

Weight (kDa)

7.96

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 8 - 60 4.6e-08 WDR5 beta-propeller domain
WD40 PF00400 9 - 46 8.6e-10 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 9 - 60 1e-06 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 10 - 61 3.2e-07 WDR3 first beta-propeller domain
EIF3I PF24805 13 - 60 6.4e-06 EIF3I
Beta-prop_TEP1_2nd PF25047 71 - 176 2e-07 TEP-1 second beta-propeller
Beta-prop_WDR3_2nd PF25172 79 - 164 4.8e-06 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 84 - 290 1.2e-45 WDR5 beta-propeller domain
WD40_Prp19 PF24814 84 - 218 6e-14 Prp19 WD40 domain
WD40 PF00400 85 - 122 1.5e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 86 - 165 2.2e-08 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 89 - 291 1.3e-25 THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 90 - 249 2.1e-07 MABP1/WDR62 second WD40 domain
WD40_WDHD1_1st PF24817 93 - 258 2.1e-14 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 104 - 220 7.2e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_ACSF4 PF13570 146 - 288 1.4e-08 Beta-alanine-activating enzyme, beta-propeller
Beta-prop_EML_2 PF23414 147 - 290 1.2e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 147 - 284 4.3e-10 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 151 - 289 1.3e-12 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 176 - 290 6.8e-12 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 177 - 290 8.3e-10 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 177 - 290 1.7e-18 WDR3 first beta-propeller domain
WDR55 PF24796 179 - 290 1.5e-11 WDR55
Beta-prop_WDR3_2nd PF25172 179 - 290 4.2e-12 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 179 - 289 8.6e-17 Prp19 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 184 - 290 2e-08 MABP1/WDR62 second WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 215 - 290 2.3e-06 CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st PF24817 227 - 290 2e-10 WDHD1 first WD40 domain
WD40 PF00400 254 - 289 3.1e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 169
Acc36I ACCTGC 1 cut(s) 360
AciI CCGC 1 cut(s) 108
AclWI GGATC 2 cut(s) 721, 750
AcoI YGGCCR 1 cut(s) 178
AcsI RAATTY 1 cut(s) 266
AfaI GTAC 2 cut(s) 39, 382
AfiI CCNNNNNNNGG 1 cut(s) 146
AgsI TTSAA 4 cut(s) 79, 307, 635, 729
AhdI GACNNNNNGTC 1 cut(s) 370
AluBI AGCT 3 cut(s) 105, 511, 582
AluI AGCT 3 cut(s) 105, 511, 582
Alw21I GWGCWC 1 cut(s) 781
Alw26I GTCTC 3 cut(s) 13, 334, 378
AlwI GGATC 2 cut(s) 721, 750
AoxI GGCC 3 cut(s) 178, 274, 646
ApeKI GCWGC 4 cut(s) 68, 105, 582, 678
ApoI RAATTY 1 cut(s) 266
Asp700I GAANNNNTTC 1 cut(s) 630
AspS9I GGNCC 1 cut(s) 274
AsuHPI GGTGA 3 cut(s) 134, 301, 803
AsuII TTCGAA 2 cut(s) 262, 481
BanII GRGCYC 1 cut(s) 390
BbsI GAAGAC 1 cut(s) 609
Bbv12I GWGCWC 1 cut(s) 781
BbvCI CCTCAGC 1 cut(s) 101
BbvI GCAGC 4 cut(s) 80, 92, 569, 665
BccI CCATC 5 cut(s) 275, 464, 554, 716, 842
BceAI ACGGC 1 cut(s) 165
BciVI GTATCC 1 cut(s) 782
BclI TGATCA 1 cut(s) 343
BcoDI GTCTC 3 cut(s) 13, 334, 378
BfaI CTAG 1 cut(s) 746
BfmI CTRYAG 2 cut(s) 367, 843
BfuAI ACCTGC 1 cut(s) 360
BfuI GTATCC 1 cut(s) 782
BglII AGATCT 1 cut(s) 13
BisI GCNGC 4 cut(s) 69, 106, 583, 679
BlsI GCNGC 4 cut(s) 70, 107, 584, 680
BmcAI AGTACT 1 cut(s) 382
BmeRI GACNNNNNGTC 1 cut(s) 370
BmgT120I GGNCC 1 cut(s) 274
BmiI GGNNCC 2 cut(s) 137, 275
BmsI GCATC 6 cut(s) 159, 255, 326, 685, 742, 764
BpiI GAAGAC 1 cut(s) 609
Bpu10I CCTNAGC 1 cut(s) 101
Bpu14I TTCGAA 2 cut(s) 262, 481
BsaI GGTCTC 1 cut(s) 378
BsaJI CCNNGG 2 cut(s) 145, 175
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse3DI GCAATG 1 cut(s) 350
BseDI CCNNGG 2 cut(s) 145, 175
BseGI GGATG 3 cut(s) 150, 245, 727
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMI GCAATG 1 cut(s) 350
BseMII CTCAG 7 cut(s) 24, 115, 345, 375, 389, 438, 708
BseRI GAGGAG 1 cut(s) 596
BseXI GCAGC 4 cut(s) 80, 92, 569, 665
Bsh1285I CGRYCG 1 cut(s) 253
BshFI GGCC 3 cut(s) 180, 276, 648
BsiEI CGRYCG 1 cut(s) 253
BsiHKAI GWGCWC 1 cut(s) 781
BslFI GGGAC 3 cut(s) 157, 377, 540
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 3 cut(s) 13, 334, 378
BsmFI GGGAC 3 cut(s) 157, 377, 540
BsmI GAATGC 1 cut(s) 298
BsnI GGCC 3 cut(s) 180, 276, 648
Bso31I GGTCTC 1 cut(s) 378
Bsp119I TTCGAA 2 cut(s) 262, 481
Bsp1286I GDGCHC 2 cut(s) 390, 781
Bsp143I GATC 4 cut(s) 13, 343, 713, 742
Bsp19I CCATGG 1 cut(s) 175
BspACI CCGC 1 cut(s) 108
BspANI GGCC 3 cut(s) 180, 276, 648
BspCNI CTCAG 7 cut(s) 23, 114, 344, 376, 388, 439, 709
BspLI GGNNCC 2 cut(s) 137, 275
BspMAI CTGCAG 1 cut(s) 371
BspMI ACCTGC 1 cut(s) 360
BspPI GGATC 2 cut(s) 721, 750
BspT104I TTCGAA 2 cut(s) 262, 481
BspTNI GGTCTC 1 cut(s) 378
BsrDI GCAATG 1 cut(s) 350
BssECI CCNNGG 2 cut(s) 145, 175
BssMI GATC 4 cut(s) 13, 343, 713, 742
BssT1I CCWWGG 1 cut(s) 175
Bst4CI ACNGT 3 cut(s) 146, 478, 542
Bst6I CTCTTC 1 cut(s) 499
BstBI TTCGAA 2 cut(s) 262, 481
BstC8I GCNNGC 2 cut(s) 357, 580
BstDEI CTNAG 8 cut(s) 10, 101, 331, 375, 384, 447, 586, 717
BstDSI CCRYGG 2 cut(s) 145, 175
BstF5I GGATG 3 cut(s) 150, 245, 727
BstKTI GATC 4 cut(s) 16, 346, 716, 745
BstMAI GTCTC 3 cut(s) 13, 334, 378
BstMBI GATC 4 cut(s) 13, 343, 713, 742
BstMCI CGRYCG 1 cut(s) 253
BstMWI GCNNNNNNNGC 2 cut(s) 65, 105
BstSFI CTRYAG 2 cut(s) 367, 843
BstV1I GCAGC 4 cut(s) 80, 92, 569, 665
BstV2I GAAGAC 1 cut(s) 609
BstX2I RGATCY 3 cut(s) 13, 713, 742
BstYI RGATCY 3 cut(s) 13, 713, 742
BsuI GTATCC 1 cut(s) 782
BsuRI GGCC 3 cut(s) 180, 276, 648
BtgI CCRYGG 2 cut(s) 145, 175
BtsCI GGATG 3 cut(s) 150, 245, 727
BtsIMutI CAGTG 2 cut(s) 690, 795
BveI ACCTGC 1 cut(s) 360
Cac8I GCNNGC 2 cut(s) 357, 580
Cfr13I GGNCC 1 cut(s) 274
Csp6I GTAC 2 cut(s) 38, 381
CviAII CATG 5 cut(s) 50, 176, 278, 701, 829
CviJI RGCY 7 cut(s) 105, 180, 276, 388, 511, 582, 648
CviKI_1 RGCY 7 cut(s) 105, 180, 276, 388, 511, 582, 648
CviQI GTAC 2 cut(s) 38, 381
DdeI CTNAG 8 cut(s) 10, 101, 331, 375, 384, 447, 586, 717
DpnI GATC 4 cut(s) 15, 345, 715, 744
DpnII GATC 4 cut(s) 13, 343, 713, 742
DrdI GACNNNNNNGTC 1 cut(s) 169
DriI GACNNNNNGTC 1 cut(s) 370
DseDI GACNNNNNNGTC 1 cut(s) 169
EaeI YGGCCR 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 499
Eam1105I GACNNNNNGTC 1 cut(s) 370
EarI CTCTTC 1 cut(s) 499
Eco130I CCWWGG 1 cut(s) 175
Eco24I GRGCYC 1 cut(s) 390
Eco31I GGTCTC 1 cut(s) 378
EcoT14I CCWWGG 1 cut(s) 175
EcoT22I ATGCAT 2 cut(s) 319, 757
EcoT38I GRGCYC 1 cut(s) 390
ErhI CCWWGG 1 cut(s) 175
FaeI CATG 5 cut(s) 53, 179, 281, 704, 832
FaqI GGGAC 3 cut(s) 157, 377, 540
FatI CATG 5 cut(s) 49, 175, 277, 700, 828
FauI CCCGC 1 cut(s) 101
FbaI TGATCA 1 cut(s) 343
Fnu4HI GCNGC 4 cut(s) 69, 106, 583, 679
FokI GGATG 3 cut(s) 137, 252, 734
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 4 cut(s) 69, 106, 583, 679
FspBI CTAG 1 cut(s) 746
GluI GCNGC 4 cut(s) 69, 106, 583, 679
HaeIII GGCC 3 cut(s) 180, 276, 648
Hin1II CATG 5 cut(s) 53, 179, 281, 704, 832
HinfI GANTC 5 cut(s) 129, 255, 404, 590, 603
HphI GGTGA 3 cut(s) 134, 301, 803
Hpy166II GTNNAC 1 cut(s) 538
Hpy188I TCNGA 7 cut(s) 13, 378, 396, 517, 710, 718, 776
Hpy188III TCNNGA 2 cut(s) 133, 635
Hpy8I GTNNAC 1 cut(s) 538
HpyAV CCTTC 2 cut(s) 19, 179
HpyCH4III ACNGT 3 cut(s) 146, 478, 542
HpyCH4V TGCA 5 cut(s) 317, 369, 570, 755, 828
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 105
HpyF3I CTNAG 8 cut(s) 10, 101, 331, 375, 384, 447, 586, 717
Hsp92II CATG 5 cut(s) 53, 179, 281, 704, 832
Ksp22I TGATCA 1 cut(s) 343
Kzo9I GATC 4 cut(s) 13, 343, 713, 742
LmnI GCTCC 1 cut(s) 56
LpnPI CCDG 7 cut(s) 118, 193, 321, 355, 546, 598, 724
Lsp1109I GCAGC 4 cut(s) 80, 92, 569, 665
LweI GCATC 6 cut(s) 159, 255, 326, 685, 742, 764
MaeI CTAG 1 cut(s) 746
MaeIII GTNAC 2 cut(s) 563, 793
MalI GATC 4 cut(s) 15, 345, 715, 744
MboI GATC 4 cut(s) 13, 343, 713, 742
MboII GAAGA 5 cut(s) 221, 516, 609, 727, 831
MflI RGATCY 3 cut(s) 13, 713, 742
MhlI GDGCHC 2 cut(s) 390, 781
MluCI AATT 8 cut(s) 91, 198, 266, 399, 453, 626, 668, 729
MlyI GAGTC 4 cut(s) 123, 264, 398, 597
MnlI CCTC 9 cut(s) 22, 46, 66, 110, 150, 278, 617, 704, 712
Mph1103I ATGCAT 2 cut(s) 319, 757
MroXI GAANNNNTTC 1 cut(s) 630
MseI TTAA 4 cut(s) 42, 159, 437, 858
MslI CAYNNNNRTG 2 cut(s) 180, 497
MspA1I CMGCKG 1 cut(s) 105
Mva1269I GAATGC 1 cut(s) 298
MwoI GCNNNNNNNGC 2 cut(s) 65, 105
NcoI CCATGG 1 cut(s) 175
NdeII GATC 4 cut(s) 13, 343, 713, 742
NlaIII CATG 5 cut(s) 53, 179, 281, 704, 832
NlaIV GGNNCC 2 cut(s) 137, 275
NsiI ATGCAT 2 cut(s) 319, 757
NspV TTCGAA 2 cut(s) 262, 481
PcsI WCGNNNNNNNCGW 1 cut(s) 259
PctI GAATGC 1 cut(s) 298
PdmI GAANNNNTTC 1 cut(s) 630
PfeI GAWTC 1 cut(s) 590
PkrI GCNGC 4 cut(s) 70, 107, 584, 680
PleI GAGTC 4 cut(s) 123, 263, 398, 597
PpsI GAGTC 4 cut(s) 123, 263, 398, 597
PspN4I GGNNCC 2 cut(s) 137, 275
PspPI GGNCC 1 cut(s) 274
PstI CTGCAG 1 cut(s) 371
PsuI RGATCY 3 cut(s) 13, 713, 742
PvuII CAGCTG 1 cut(s) 105
RsaI GTAC 2 cut(s) 39, 382
RsaNI GTAC 2 cut(s) 38, 381
RseI CAYNNNNRTG 2 cut(s) 180, 497
SaqAI TTAA 4 cut(s) 42, 159, 437, 858
SatI GCNGC 4 cut(s) 69, 106, 583, 679
Sau3AI GATC 4 cut(s) 13, 343, 713, 742
Sau96I GGNCC 1 cut(s) 274
ScaI AGTACT 1 cut(s) 382
SchI GAGTC 4 cut(s) 123, 264, 398, 597
SduI GDGCHC 2 cut(s) 390, 781
SfaNI GCATC 6 cut(s) 159, 255, 326, 685, 742, 764
SfcI CTRYAG 2 cut(s) 367, 843
SfuI TTCGAA 2 cut(s) 262, 481
SmiMI CAYNNNNRTG 2 cut(s) 180, 497
Sse9I AATT 8 cut(s) 91, 198, 266, 399, 453, 626, 668, 729
SsiI CCGC 1 cut(s) 108
SspMI CTAG 1 cut(s) 746
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 3 cut(s) 146, 478, 542
TaqI TCGA 5 cut(s) 253, 262, 481, 593, 609
TaqII GACCGA 2 cut(s) 132, 239
TasI AATT 8 cut(s) 91, 198, 266, 399, 453, 626, 668, 729
TatI WGTACW 2 cut(s) 37, 380
TfiI GAWTC 1 cut(s) 590
Tru1I TTAA 4 cut(s) 42, 159, 437, 858
Tru9I TTAA 4 cut(s) 42, 159, 437, 858
TscAI CASTG 2 cut(s) 697, 795
TseI GCWGC 4 cut(s) 68, 105, 582, 678
TspGWI ACGGA 1 cut(s) 102
TspRI CASTG 2 cut(s) 697, 795
XapI RAATTY 1 cut(s) 266
XmnI GAANNNNTTC 1 cut(s) 630
XspI CTAG 1 cut(s) 746
ZrmI AGTACT 1 cut(s) 382
Zsp2I ATGCAT 2 cut(s) 319, 757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.