Rh6DG085500

WD repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0005405
Physical Location & Seq
Reverse (-)
10008185 .. 10012169
3985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG085500.1

Sequence Viewer

Length: 975 bp
ATGAAAGCGAGCTTTACAGAAAATACCCAGTTGGCCCCCTCAACAAGGAGTGAGCAGAGAGCAAGTAGGAAGGAGATGAGTGTCTCAGATCTGCCAAAGAAGGAGGTAAATGTACTTAAAGGGCATGAGGGAGCAGTATTAGCAGCGAGGTTCAATAAAGACGGAAATTACTGCCTCAGCTGCGGGAAAGACCGAACCATACGACTCTGGAACCCTCACCGTGGCATCCACATTAAGACCTACAAGTCCCATGGCCGTGAAGTCCGTGATGTCCATGTCACCTTGGACAACTCCAAATTATGTTCAGGTGGTGGCGACAGACAAATCTTCTATTGGGATGTGGCATCTGGTCGAGTCATTCGAAAATTTCGGGGCCATGATGGAGGGGTGAATGCTGTGAAGTTCAATGAGTATGCATCTGTTGTAGTCTCAGCAGGATATGATCAGTCATTGCGTGCTTGGGACTGCAGGTCTCAGAGTACTGAGCCTATTCAGATAATTGACTCGTTTACAGATAGTGTAATGTCTGTTTGTTTAACAAAGACTGAGATAATTGGTGGAAGTGTTGATGGTACTGTTCGAACATTTGACATTCGTATGGGAAGAGAGCTAGCCGATGACTTGGGACAACCTGTGAACTGTATCTCAATGTCAAATGATGGTAACTGCATATTAGCAAGCTGCTTAGATTCGACTATACGACTCCTCGACAGGTCTTCCGCTGAATTACTTCAAGAATATAAAGGCCATACTTGTAAGTCTTACAAATTGGACTGCTGCCTTACCAACACTGATGCCCATGTAATCGGAGGATCTGAGGATGGTTCAATTTTCTTCTGGGATCTAGTAGATGCATCTGTTGTGTCAAGTATCCGAGCACATTCGTCAGTGGTTACAAGTGTCAGTTATCACCCAAAAGACAACTGCATGGTTAGTTCTTCTGTAGATGGCACTATTAAGGTGTGGAAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

324

Amino Acids

35.69

Weight (kDa)

7.55

Isoelectric Point (pI)

42.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EML PF23409 26 - 183 9.8e-08 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3 PF25174 26 - 120 2.2e-13 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 28 - 96 1.8e-09 WDR3 first beta-propeller domain
WDR55 PF24796 33 - 198 3.5e-14 WDR55
WD40_Prp19 PF24814 33 - 93 3e-07 Prp19 WD40 domain
WD40 PF00400 34 - 71 1e-09 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 35 - 156 1.9e-13 TEP-1 second beta-propeller
Beta-prop_WDR3_2nd PF25172 38 - 155 3.1e-15 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 38 - 173 2.5e-12 G protein beta WD-40 repeat protein
EIF3I PF24805 38 - 250 4e-24 EIF3I
Beta-prop_WDR5 PF25175 39 - 323 8.9e-66 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 41 - 172 2.2e-11 WDR36/Utp21 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 53 - 154 1.3e-07 MABP1/WDR62 second WD40 domain
Beta-prop_SCAP PF24017 62 - 158 1e-06 SCAP Beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 65 - 154 5.6e-06 WDR36/Utp21 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 76 - 160 3.8e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR41 PF25178 78 - 180 9.1e-07 WDR41
WD40_CDC20-Fz PF24807 78 - 160 8.9e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 80 - 198 1.6e-18 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 84 - 324 7.1e-32 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 87 - 162 4.9e-08 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 87 - 197 6.5e-10 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 96 - 251 1.5e-20 Prp19 WD40 domain
WD40 PF00400 118 - 155 1.7e-06 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 122 - 209 7e-07 TEP-1 second beta-propeller
WD40_MABP1-WDR62_2nd PF24782 122 - 282 3.8e-07 MABP1/WDR62 second WD40 domain
Beta-prop_EML_2 PF23414 138 - 254 5.3e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 171 - 290 2.2e-08 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 180 - 317 6.5e-10 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 180 - 323 1.9e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 184 - 322 1.7e-12 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 209 - 323 7.9e-12 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 210 - 323 1.1e-09 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 210 - 323 2.3e-18 WDR3 first beta-propeller domain
WDR55 PF24796 211 - 323 1.8e-11 WDR55
Beta-prop_WDR3_2nd PF25172 212 - 323 5.2e-12 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 212 - 322 1.1e-16 Prp19 WD40 domain
Beta-prop_ACSF4 PF13570 216 - 321 3e-07 Beta-alanine-activating enzyme, beta-propeller
WD40_MABP1-WDR62_2nd PF24782 217 - 323 2.5e-08 MABP1/WDR62 second WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 248 - 323 2.8e-06 CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st PF24817 260 - 323 2.2e-10 WDHD1 first WD40 domain
WD40 PF00400 287 - 322 3.7e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 244
Acc36I ACCTGC 1 cut(s) 459
AciI CCGC 2 cut(s) 183, 720
AclWI GGATC 2 cut(s) 820, 849
AcoI YGGCCR 1 cut(s) 253
AcsI RAATTY 1 cut(s) 365
AfaI GTAC 3 cut(s) 114, 481, 574
AfiI CCNNNNNNNGG 2 cut(s) 45, 221
AgsI TTSAA 4 cut(s) 154, 406, 734, 828
AhdI GACNNNNNGTC 1 cut(s) 469
AluBI AGCT 4 cut(s) 12, 180, 610, 681
AluI AGCT 4 cut(s) 12, 180, 610, 681
Alw21I GWGCWC 1 cut(s) 880
Alw26I GTCTC 3 cut(s) 88, 433, 477
AlwI GGATC 2 cut(s) 820, 849
AoxI GGCC 4 cut(s) 33, 253, 373, 745
ApeKI GCWGC 4 cut(s) 143, 180, 681, 777
ApoI RAATTY 1 cut(s) 365
Asp700I GAANNNNTTC 1 cut(s) 729
AspS9I GGNCC 2 cut(s) 34, 373
AsuHPI GGTGA 4 cut(s) 209, 271, 400, 902
AsuII TTCGAA 2 cut(s) 361, 580
AsuNHI GCTAGC 1 cut(s) 610
BbsI GAAGAC 1 cut(s) 708
Bbv12I GWGCWC 1 cut(s) 880
BbvCI CCTCAGC 1 cut(s) 176
BbvI GCAGC 4 cut(s) 155, 167, 668, 764
BccI CCATC 5 cut(s) 374, 563, 653, 815, 941
BceAI ACGGC 1 cut(s) 240
BciVI GTATCC 1 cut(s) 881
BclI TGATCA 1 cut(s) 442
BcoDI GTCTC 3 cut(s) 88, 433, 477
BfaI CTAG 2 cut(s) 611, 845
BfmI CTRYAG 2 cut(s) 466, 942
BfuAI ACCTGC 1 cut(s) 459
BfuI GTATCC 1 cut(s) 881
BglII AGATCT 1 cut(s) 88
BisI GCNGC 4 cut(s) 144, 181, 682, 778
BlsI GCNGC 4 cut(s) 145, 182, 683, 779
BmcAI AGTACT 1 cut(s) 481
BmeRI GACNNNNNGTC 1 cut(s) 469
BmgT120I GGNCC 2 cut(s) 34, 373
BmiI GGNNCC 3 cut(s) 36, 212, 374
BmrI ACTGGG 1 cut(s) 22
BmsI GCATC 6 cut(s) 234, 353, 425, 784, 841, 863
BmtI GCTAGC 1 cut(s) 614
BmuI ACTGGG 1 cut(s) 22
BpiI GAAGAC 1 cut(s) 708
Bpu10I CCTNAGC 1 cut(s) 176
Bpu14I TTCGAA 2 cut(s) 361, 580
BsaI GGTCTC 1 cut(s) 477
BsaJI CCNNGG 3 cut(s) 220, 250, 282
Bsc4I CCNNNNNNNGG 2 cut(s) 45, 221
Bse1I ACTGG 1 cut(s) 28
Bse3DI GCAATG 1 cut(s) 449
BseDI CCNNGG 3 cut(s) 220, 250, 282
BseGI GGATG 3 cut(s) 225, 343, 826
BseLI CCNNNNNNNGG 2 cut(s) 45, 221
BseMI GCAATG 1 cut(s) 449
BseMII CTCAG 7 cut(s) 99, 190, 444, 474, 488, 537, 807
BseNI ACTGG 1 cut(s) 28
BseRI GAGGAG 1 cut(s) 695
BseXI GCAGC 4 cut(s) 155, 167, 668, 764
BshFI GGCC 4 cut(s) 35, 255, 375, 747
BsiHKAI GWGCWC 1 cut(s) 880
BslFI GGGAC 3 cut(s) 232, 476, 639
BslI CCNNNNNNNGG 2 cut(s) 45, 221
BsmAI GTCTC 3 cut(s) 88, 433, 477
BsmFI GGGAC 3 cut(s) 232, 476, 639
BsmI GAATGC 1 cut(s) 397
BsnI GGCC 4 cut(s) 35, 255, 375, 747
Bso31I GGTCTC 1 cut(s) 477
Bsp119I TTCGAA 2 cut(s) 361, 580
Bsp1286I GDGCHC 1 cut(s) 880
Bsp143I GATC 4 cut(s) 88, 442, 812, 841
Bsp19I CCATGG 1 cut(s) 250
BspACI CCGC 2 cut(s) 183, 720
BspANI GGCC 4 cut(s) 35, 255, 375, 747
BspCNI CTCAG 7 cut(s) 98, 189, 443, 475, 487, 538, 808
BspLI GGNNCC 3 cut(s) 36, 212, 374
BspMAI CTGCAG 1 cut(s) 470
BspMI ACCTGC 1 cut(s) 459
BspOI GCTAGC 1 cut(s) 614
BspPI GGATC 2 cut(s) 820, 849
BspT104I TTCGAA 2 cut(s) 361, 580
BspTNI GGTCTC 1 cut(s) 477
BsrDI GCAATG 1 cut(s) 449
BsrI ACTGG 1 cut(s) 28
BssECI CCNNGG 3 cut(s) 220, 250, 282
BssMI GATC 4 cut(s) 88, 442, 812, 841
BssT1I CCWWGG 2 cut(s) 250, 282
Bst4CI ACNGT 3 cut(s) 221, 577, 641
Bst6I CTCTTC 1 cut(s) 598
BstBI TTCGAA 2 cut(s) 361, 580
BstC8I GCNNGC 4 cut(s) 10, 456, 612, 679
BstDEI CTNAG 8 cut(s) 85, 176, 430, 474, 483, 546, 685, 816
BstDSI CCRYGG 2 cut(s) 220, 250
BstENI CCTNNNNNAGG 1 cut(s) 43
BstF5I GGATG 3 cut(s) 225, 343, 826
BstKTI GATC 4 cut(s) 91, 445, 815, 844
BstMAI GTCTC 3 cut(s) 88, 433, 477
BstMBI GATC 4 cut(s) 88, 442, 812, 841
BstMWI GCNNNNNNNGC 2 cut(s) 140, 180
BstSFI CTRYAG 2 cut(s) 466, 942
BstV1I GCAGC 4 cut(s) 155, 167, 668, 764
BstV2I GAAGAC 1 cut(s) 708
BstX2I RGATCY 3 cut(s) 88, 812, 841
BstYI RGATCY 3 cut(s) 88, 812, 841
BsuI GTATCC 1 cut(s) 881
BsuRI GGCC 4 cut(s) 35, 255, 375, 747
BtgI CCRYGG 2 cut(s) 220, 250
BtsCI GGATG 3 cut(s) 225, 343, 826
BtsIMutI CAGTG 2 cut(s) 789, 894
BveI ACCTGC 1 cut(s) 459
Cac8I GCNNGC 4 cut(s) 10, 456, 612, 679
Cfr13I GGNCC 2 cut(s) 34, 373
Csp6I GTAC 3 cut(s) 113, 480, 573
CviAII CATG 6 cut(s) 125, 251, 275, 377, 800, 928
CviQI GTAC 3 cut(s) 113, 480, 573
DdeI CTNAG 8 cut(s) 85, 176, 430, 474, 483, 546, 685, 816
DpnI GATC 4 cut(s) 90, 444, 814, 843
DpnII GATC 4 cut(s) 88, 442, 812, 841
DrdI GACNNNNNNGTC 1 cut(s) 244
DriI GACNNNNNGTC 1 cut(s) 469
DseDI GACNNNNNNGTC 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 253
Eam1104I CTCTTC 1 cut(s) 598
Eam1105I GACNNNNNGTC 1 cut(s) 469
EarI CTCTTC 1 cut(s) 598
Eco130I CCWWGG 2 cut(s) 250, 282
Eco31I GGTCTC 1 cut(s) 477
EcoNI CCTNNNNNAGG 1 cut(s) 43
EcoT14I CCWWGG 2 cut(s) 250, 282
EcoT22I ATGCAT 2 cut(s) 418, 856
ErhI CCWWGG 2 cut(s) 250, 282
FaeI CATG 6 cut(s) 128, 254, 278, 380, 803, 931
FaqI GGGAC 3 cut(s) 232, 476, 639
FatI CATG 6 cut(s) 124, 250, 274, 376, 799, 927
FauI CCCGC 1 cut(s) 176
FbaI TGATCA 1 cut(s) 442
Fnu4HI GCNGC 4 cut(s) 144, 181, 682, 778
FokI GGATG 3 cut(s) 212, 350, 833
Fsp4HI GCNGC 4 cut(s) 144, 181, 682, 778
FspBI CTAG 2 cut(s) 611, 845
GluI GCNGC 4 cut(s) 144, 181, 682, 778
HaeIII GGCC 4 cut(s) 35, 255, 375, 747
Hin1II CATG 6 cut(s) 128, 254, 278, 380, 803, 931
HinfI GANTC 5 cut(s) 204, 354, 503, 689, 702
HphI GGTGA 4 cut(s) 209, 271, 400, 902
Hpy166II GTNNAC 2 cut(s) 510, 637
Hpy188I TCNGA 6 cut(s) 88, 477, 495, 809, 817, 875
Hpy188III TCNNGA 2 cut(s) 208, 734
Hpy8I GTNNAC 2 cut(s) 510, 637
HpyAV CCTTC 2 cut(s) 64, 94
HpyCH4III ACNGT 3 cut(s) 221, 577, 641
HpyCH4V TGCA 5 cut(s) 416, 468, 669, 854, 927
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 180
HpyF3I CTNAG 8 cut(s) 85, 176, 430, 474, 483, 546, 685, 816
Hsp92II CATG 6 cut(s) 128, 254, 278, 380, 803, 931
Ksp22I TGATCA 1 cut(s) 442
Kzo9I GATC 4 cut(s) 88, 442, 812, 841
LmnI GCTCC 1 cut(s) 131
LpnPI CCDG 9 cut(s) 41, 193, 291, 333, 420, 454, 645, 697, 823
Lsp1109I GCAGC 4 cut(s) 155, 167, 668, 764
LweI GCATC 6 cut(s) 234, 353, 425, 784, 841, 863
MaeI CTAG 2 cut(s) 611, 845
MaeIII GTNAC 3 cut(s) 277, 662, 892
MalI GATC 4 cut(s) 90, 444, 814, 843
MboI GATC 4 cut(s) 88, 442, 812, 841
MboII GAAGA 5 cut(s) 319, 615, 708, 826, 930
MflI RGATCY 3 cut(s) 88, 812, 841
MhlI GDGCHC 1 cut(s) 880
MluCI AATT 8 cut(s) 166, 296, 365, 498, 552, 725, 767, 828
MlyI GAGTC 4 cut(s) 198, 363, 497, 696
Mph1103I ATGCAT 2 cut(s) 418, 856
MroXI GAANNNNTTC 1 cut(s) 729
MseI TTAA 4 cut(s) 117, 234, 536, 957
MslI CAYNNNNRTG 2 cut(s) 255, 596
MspA1I CMGCKG 2 cut(s) 180, 722
Mva1269I GAATGC 1 cut(s) 397
MwoI GCNNNNNNNGC 2 cut(s) 140, 180
NcoI CCATGG 1 cut(s) 250
NdeII GATC 4 cut(s) 88, 442, 812, 841
NheI GCTAGC 1 cut(s) 610
NlaIII CATG 6 cut(s) 128, 254, 278, 380, 803, 931
NlaIV GGNNCC 3 cut(s) 36, 212, 374
NmuCI GTSAC 1 cut(s) 277
NsiI ATGCAT 2 cut(s) 418, 856
NspV TTCGAA 2 cut(s) 361, 580
PcsI WCGNNNNNNNCGW 1 cut(s) 358
PctI GAATGC 1 cut(s) 397
PdmI GAANNNNTTC 1 cut(s) 729
PfeI GAWTC 1 cut(s) 689
PkrI GCNGC 4 cut(s) 145, 182, 683, 779
PleI GAGTC 4 cut(s) 198, 362, 497, 696
PpsI GAGTC 4 cut(s) 198, 362, 497, 696
PspN4I GGNNCC 3 cut(s) 36, 212, 374
PspPI GGNCC 2 cut(s) 34, 373
PstI CTGCAG 1 cut(s) 470
PsuI RGATCY 3 cut(s) 88, 812, 841
PvuII CAGCTG 1 cut(s) 180
RsaI GTAC 3 cut(s) 114, 481, 574
RsaNI GTAC 3 cut(s) 113, 480, 573
RseI CAYNNNNRTG 2 cut(s) 255, 596
SaqAI TTAA 4 cut(s) 117, 234, 536, 957
SatI GCNGC 4 cut(s) 144, 181, 682, 778
Sau3AI GATC 4 cut(s) 88, 442, 812, 841
Sau96I GGNCC 2 cut(s) 34, 373
ScaI AGTACT 1 cut(s) 481
SchI GAGTC 4 cut(s) 198, 363, 497, 696
SduI GDGCHC 1 cut(s) 880
SfaNI GCATC 6 cut(s) 234, 353, 425, 784, 841, 863
SfcI CTRYAG 2 cut(s) 466, 942
SfuI TTCGAA 2 cut(s) 361, 580
SmiMI CAYNNNNRTG 2 cut(s) 255, 596
Sse9I AATT 8 cut(s) 166, 296, 365, 498, 552, 725, 767, 828
SsiI CCGC 2 cut(s) 183, 720
SspMI CTAG 2 cut(s) 611, 845
StyI CCWWGG 2 cut(s) 250, 282
TaaI ACNGT 3 cut(s) 221, 577, 641
TaqI TCGA 5 cut(s) 352, 361, 580, 692, 708
TaqII GACCGA 1 cut(s) 207
TasI AATT 8 cut(s) 166, 296, 365, 498, 552, 725, 767, 828
TatI WGTACW 2 cut(s) 112, 479
TfiI GAWTC 1 cut(s) 689
Tru1I TTAA 4 cut(s) 117, 234, 536, 957
Tru9I TTAA 4 cut(s) 117, 234, 536, 957
TscAI CASTG 2 cut(s) 796, 894
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 4 cut(s) 143, 180, 681, 777
Tsp45I GTSAC 1 cut(s) 277
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 177, 254
TspRI CASTG 2 cut(s) 796, 894
XagI CCTNNNNNAGG 1 cut(s) 43
XapI RAATTY 1 cut(s) 365
XmnI GAANNNNTTC 1 cut(s) 729
XspI CTAG 2 cut(s) 611, 845
ZrmI AGTACT 1 cut(s) 481
Zsp2I ATGCAT 2 cut(s) 418, 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.