RLG00000018493

WD repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005405
Physical Location & Seq
Forward (+)
30751795 .. 30757229
5435 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018493

Sequence Viewer

Length: 1038 bp
ATGAGGATGATTAATTGGAGTGGTCGCCAGCTGGATTTGGTGTCGCCGAGGCTGGCTCAGGAGGCGAAAGCGAGCTTTACAGAAAATACCCAGTTGGCCCCCTCAACAAGTAGTGAGCAGAGAGCAAGTAGGAAGGAGATGAGTGTCTCAGATCTACCAAAGAAGGAGGTAAATGTACTTAAAGGGCATGAGGGAGCAGTATTAGCAGCAAGGTTCAATAAAGACGGAAATTACTGCCTCAGCTGCGGGAAAGACCGAACCATCCGACTCTGGAACCCTCACCGTGGCATCCACATTAAGACCTACAAGTCCCATGGCCGTGAAGTCCGTGATGTCCATGTCACCTTGGACAACTCCAAATTATGTTCAGGTGGTGGCGACAGACAAATCTTCTATTGGGATGTGGCATCCGGTCGAGTCATTCGAAAATTTCGGGGCCATGATGGAGGGGTGAATGCTGTGAAGTTCAATGAGTATGCATCTGTTGTAGTCTCAGCAGGATATGATCAGTCATTGCGTGCTTGGGACTGCAGGTCTCAGAGTACTGAGCCTATTCAGATAATTGACTCGTTTACAGATAGTGTAATGTCTGTTTGTTTAACAAAGACTGAGATAATTGGTGGAAGTGTTGATGGAACTGTTCGAACATTTGACATTCGTATGGGAAGAGAGCTATCCGATGACTTGGGACAACCTGTGAACTGTATCTCAATGTCAAATGATGGTAACTGCATATTAGCAAGCTGCTTAGATTCGACTATACGACTCCTCGACAGGTCTTCCGCTGAATTACTTCAAGAATACAAAGGCCATACGTGTAAGTCTTACAAATTGGACTGCTGCCTTACCAACACTGATGCCCATGTAATCGGAGGATCTGAGGATGGTTCAATTTTCTTCTGGGATCTAGTAGATGCATCTGTTGTGTCAAGTATCCGAGCACATTCGTCAGTGGTTACTAGTGTCAGTTATCACCCAAAGGACAACTGCATGGTTAGTTCATCAGTAGATGGCACTATTAAGGTGTGGAAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

346

Amino Acids

38.06

Weight (kDa)

7.55

Isoelectric Point (pI)

41.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 45 - 140 2.3e-13 THOC3 beta-propeller domain
Beta-prop_EML PF23409 47 - 204 1.2e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st PF25173 49 - 117 1.7e-09 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 50 - 178 8.6e-14 TEP-1 second beta-propeller
WDR55 PF24796 54 - 227 2.4e-14 WDR55
WD40_Prp19 PF24814 54 - 114 3.4e-07 Prp19 WD40 domain
WD40 PF00400 55 - 92 1.1e-09 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 59 - 176 3.5e-15 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 59 - 198 2.6e-12 G protein beta WD-40 repeat protein
EIF3I PF24805 59 - 271 4.7e-24 EIF3I
Beta-prop_WDR5 PF25175 60 - 344 1.7e-65 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 62 - 193 2.2e-11 WDR36/Utp21 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 74 - 175 1.5e-07 MABP1/WDR62 second WD40 domain
Beta-prop_SCAP PF24017 83 - 179 8e-07 SCAP Beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 87 - 175 6.4e-06 WDR36/Utp21 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 97 - 181 4.5e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR41 PF25178 99 - 201 1.1e-06 WDR41
WD40_CDC20-Fz PF24807 99 - 181 1e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 101 - 219 1.9e-18 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 105 - 345 9.6e-32 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 108 - 183 5.4e-08 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 108 - 218 8.2e-10 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 117 - 272 1.8e-20 Prp19 WD40 domain
WD40 PF00400 139 - 176 1.9e-06 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 143 - 230 8.6e-07 TEP-1 second beta-propeller
WD40_MABP1-WDR62_2nd PF24782 144 - 303 4.1e-07 MABP1/WDR62 second WD40 domain
Beta-prop_EML_2 PF23414 159 - 274 1e-05 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 192 - 312 2.7e-08 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 201 - 338 6.7e-10 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 201 - 344 1.8e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 205 - 343 2e-12 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 230 - 344 8.7e-12 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 231 - 344 1.2e-09 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 231 - 344 2.7e-18 WDR3 first beta-propeller domain
WDR55 PF24796 233 - 344 2.2e-11 WDR55
WD40_Prp19 PF24814 233 - 343 1.2e-16 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 233 - 344 5.6e-12 WDR3 second beta-propeller domain
Beta-prop_ACSF4 PF13570 237 - 342 3.4e-07 Beta-alanine-activating enzyme, beta-propeller
WD40_MABP1-WDR62_2nd PF24782 238 - 344 4.2e-08 MABP1/WDR62 second WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 269 - 344 2.7e-06 CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st PF24817 281 - 344 2.3e-10 WDHD1 first WD40 domain
WD40 PF00400 308 - 343 4e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 307
Acc36I ACCTGC 1 cut(s) 522
AciI CCGC 2 cut(s) 246, 783
AclWI GGATC 2 cut(s) 883, 912
AcoI YGGCCR 1 cut(s) 316
AcsI RAATTY 1 cut(s) 428
AfaI GTAC 2 cut(s) 177, 544
AfiI CCNNNNNNNGG 1 cut(s) 284
AflIII ACRYGT 1 cut(s) 815
AgsI TTSAA 4 cut(s) 217, 469, 797, 891
AhdI GACNNNNNGTC 1 cut(s) 532
AhlI ACTAGT 1 cut(s) 959
AluBI AGCT 5 cut(s) 31, 75, 243, 673, 744
AluI AGCT 5 cut(s) 31, 75, 243, 673, 744
Alw21I GWGCWC 1 cut(s) 943
Alw26I GTCTC 3 cut(s) 151, 496, 540
AlwI GGATC 2 cut(s) 883, 912
AoxI GGCC 4 cut(s) 96, 316, 436, 808
ApeKI GCWGC 4 cut(s) 206, 243, 744, 840
ApoI RAATTY 1 cut(s) 428
AseI ATTAAT 1 cut(s) 12
Asp700I GAANNNNTTC 1 cut(s) 792
AspS9I GGNCC 2 cut(s) 97, 436
AsuHPI GGTGA 4 cut(s) 272, 334, 463, 965
AsuII TTCGAA 2 cut(s) 424, 643
BbsI GAAGAC 1 cut(s) 771
Bbv12I GWGCWC 1 cut(s) 943
BbvCI CCTCAGC 1 cut(s) 239
BbvI GCAGC 4 cut(s) 218, 230, 731, 827
BccI CCATC 6 cut(s) 269, 437, 626, 716, 878, 1004
BceAI ACGGC 1 cut(s) 303
BciVI GTATCC 1 cut(s) 944
BclI TGATCA 1 cut(s) 505
BcoDI GTCTC 3 cut(s) 151, 496, 540
BcuI ACTAGT 1 cut(s) 959
BfaI CTAG 2 cut(s) 908, 960
BfmI CTRYAG 1 cut(s) 529
BfuAI ACCTGC 1 cut(s) 522
BfuI GTATCC 1 cut(s) 944
BglII AGATCT 1 cut(s) 151
BisI GCNGC 4 cut(s) 207, 244, 745, 841
BlsI GCNGC 4 cut(s) 208, 245, 746, 842
BmcAI AGTACT 1 cut(s) 544
BmeRI GACNNNNNGTC 1 cut(s) 532
BmgT120I GGNCC 2 cut(s) 97, 436
BmiI GGNNCC 3 cut(s) 99, 275, 437
BmrI ACTGGG 1 cut(s) 85
BmsI GCATC 6 cut(s) 297, 416, 488, 847, 904, 926
BmuI ACTGGG 1 cut(s) 85
BpiI GAAGAC 1 cut(s) 771
BplI GAGNNNNNCTC 2 cut(s) 40, 72
Bpu10I CCTNAGC 2 cut(s) 57, 239
Bpu14I TTCGAA 2 cut(s) 424, 643
BsaAI YACGTR 1 cut(s) 816
BsaI GGTCTC 1 cut(s) 540
BsaJI CCNNGG 4 cut(s) 47, 283, 313, 345
BsaWI WCCGGW 1 cut(s) 410
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse1I ACTGG 1 cut(s) 91
Bse3DI GCAATG 1 cut(s) 512
BseDI CCNNGG 4 cut(s) 47, 283, 313, 345
BseGI GGATG 6 cut(s) 12, 261, 288, 406, 407, 889
BseLI CCNNNNNNNGG 1 cut(s) 284
BseMI GCAATG 1 cut(s) 512
BseMII CTCAG 8 cut(s) 71, 162, 253, 507, 537, 551, 600, 870
BseNI ACTGG 1 cut(s) 91
BseRI GAGGAG 1 cut(s) 758
BseXI GCAGC 4 cut(s) 218, 230, 731, 827
Bsh1285I CGRYCG 1 cut(s) 415
BshFI GGCC 4 cut(s) 98, 318, 438, 810
BsiEI CGRYCG 1 cut(s) 415
BsiHKAI GWGCWC 1 cut(s) 943
BsiSI CCGG 1 cut(s) 411
BslFI GGGAC 3 cut(s) 295, 539, 702
BslI CCNNNNNNNGG 1 cut(s) 284
BsmAI GTCTC 3 cut(s) 151, 496, 540
BsmFI GGGAC 3 cut(s) 295, 539, 702
BsmI GAATGC 1 cut(s) 460
BsnI GGCC 4 cut(s) 98, 318, 438, 810
Bso31I GGTCTC 1 cut(s) 540
Bsp119I TTCGAA 2 cut(s) 424, 643
Bsp1286I GDGCHC 1 cut(s) 943
Bsp143I GATC 4 cut(s) 151, 505, 875, 904
Bsp19I CCATGG 1 cut(s) 313
BspACI CCGC 2 cut(s) 246, 783
BspANI GGCC 4 cut(s) 98, 318, 438, 810
BspCNI CTCAG 8 cut(s) 70, 161, 252, 506, 538, 550, 601, 871
BspLI GGNNCC 3 cut(s) 99, 275, 437
BspMAI CTGCAG 1 cut(s) 533
BspMI ACCTGC 1 cut(s) 522
BspPI GGATC 2 cut(s) 883, 912
BspT104I TTCGAA 2 cut(s) 424, 643
BspTNI GGTCTC 1 cut(s) 540
BsrDI GCAATG 1 cut(s) 512
BsrI ACTGG 1 cut(s) 91
BssECI CCNNGG 4 cut(s) 47, 283, 313, 345
BssMI GATC 4 cut(s) 151, 505, 875, 904
BssT1I CCWWGG 2 cut(s) 313, 345
Bst4CI ACNGT 3 cut(s) 284, 640, 704
Bst6I CTCTTC 1 cut(s) 661
BstBAI YACGTR 1 cut(s) 816
BstBI TTCGAA 2 cut(s) 424, 643
BstC8I GCNNGC 5 cut(s) 29, 54, 73, 519, 742
BstDEI CTNAG 9 cut(s) 57, 148, 239, 493, 537, 546, 609, 748, 879
BstDSI CCRYGG 2 cut(s) 283, 313
BstF5I GGATG 6 cut(s) 12, 261, 288, 406, 407, 889
BstKTI GATC 4 cut(s) 154, 508, 878, 907
BstMAI GTCTC 3 cut(s) 151, 496, 540
BstMBI GATC 4 cut(s) 151, 505, 875, 904
BstMCI CGRYCG 1 cut(s) 415
BstMWI GCNNNNNNNGC 3 cut(s) 62, 203, 243
BstSFI CTRYAG 1 cut(s) 529
BstV1I GCAGC 4 cut(s) 218, 230, 731, 827
BstV2I GAAGAC 1 cut(s) 771
BstX2I RGATCY 3 cut(s) 151, 875, 904
BstYI RGATCY 3 cut(s) 151, 875, 904
BsuI GTATCC 1 cut(s) 944
BsuRI GGCC 4 cut(s) 98, 318, 438, 810
BtgI CCRYGG 2 cut(s) 283, 313
BtsCI GGATG 6 cut(s) 12, 261, 288, 406, 407, 889
BtsIMutI CAGTG 2 cut(s) 852, 957
BveI ACCTGC 1 cut(s) 522
Cac8I GCNNGC 5 cut(s) 29, 54, 73, 519, 742
Cfr13I GGNCC 2 cut(s) 97, 436
Csp6I GTAC 2 cut(s) 176, 543
CviAII CATG 6 cut(s) 188, 314, 338, 440, 863, 991
CviQI GTAC 2 cut(s) 176, 543
DdeI CTNAG 9 cut(s) 57, 148, 239, 493, 537, 546, 609, 748, 879
DpnI GATC 4 cut(s) 153, 507, 877, 906
DpnII GATC 4 cut(s) 151, 505, 875, 904
DrdI GACNNNNNNGTC 1 cut(s) 307
DriI GACNNNNNGTC 1 cut(s) 532
DseDI GACNNNNNNGTC 1 cut(s) 307
EaeI YGGCCR 1 cut(s) 316
Eam1104I CTCTTC 1 cut(s) 661
Eam1105I GACNNNNNGTC 1 cut(s) 532
EarI CTCTTC 1 cut(s) 661
Eco130I CCWWGG 2 cut(s) 313, 345
Eco31I GGTCTC 1 cut(s) 540
EcoT14I CCWWGG 2 cut(s) 313, 345
EcoT22I ATGCAT 2 cut(s) 481, 919
ErhI CCWWGG 2 cut(s) 313, 345
FaeI CATG 6 cut(s) 191, 317, 341, 443, 866, 994
FaqI GGGAC 3 cut(s) 295, 539, 702
FatI CATG 6 cut(s) 187, 313, 337, 439, 862, 990
FauI CCCGC 1 cut(s) 239
FbaI TGATCA 1 cut(s) 505
Fnu4HI GCNGC 4 cut(s) 207, 244, 745, 841
FokI GGATG 6 cut(s) 19, 248, 275, 394, 413, 896
Fsp4HI GCNGC 4 cut(s) 207, 244, 745, 841
FspBI CTAG 2 cut(s) 908, 960
GluI GCNGC 4 cut(s) 207, 244, 745, 841
HaeIII GGCC 4 cut(s) 98, 318, 438, 810
HapII CCGG 1 cut(s) 411
Hin1II CATG 6 cut(s) 191, 317, 341, 443, 866, 994
HinfI GANTC 5 cut(s) 267, 417, 566, 752, 765
HpaII CCGG 1 cut(s) 411
HphI GGTGA 4 cut(s) 272, 334, 463, 965
Hpy166II GTNNAC 2 cut(s) 573, 700
Hpy188I TCNGA 8 cut(s) 151, 266, 540, 558, 679, 872, 880, 938
Hpy188III TCNNGA 3 cut(s) 59, 271, 797
Hpy8I GTNNAC 2 cut(s) 573, 700
HpyAV CCTTC 2 cut(s) 127, 157
HpyCH4III ACNGT 3 cut(s) 284, 640, 704
HpyCH4IV ACGT 1 cut(s) 815
HpyCH4V TGCA 5 cut(s) 479, 531, 732, 917, 990
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 203, 243
HpyF3I CTNAG 9 cut(s) 57, 148, 239, 493, 537, 546, 609, 748, 879
HpySE526I ACGT 1 cut(s) 815
Hsp92II CATG 6 cut(s) 191, 317, 341, 443, 866, 994
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 4 cut(s) 151, 505, 875, 904
LmnI GCTCC 1 cut(s) 194
Lsp1109I GCAGC 4 cut(s) 218, 230, 731, 827
LweI GCATC 6 cut(s) 297, 416, 488, 847, 904, 926
MaeI CTAG 2 cut(s) 908, 960
MaeII ACGT 1 cut(s) 815
MaeIII GTNAC 3 cut(s) 340, 725, 955
MalI GATC 4 cut(s) 153, 507, 877, 906
MboI GATC 4 cut(s) 151, 505, 875, 904
MboII GAAGA 4 cut(s) 382, 678, 771, 889
MflI RGATCY 3 cut(s) 151, 875, 904
MhlI GDGCHC 1 cut(s) 943
MluCI AATT 9 cut(s) 13, 229, 359, 428, 561, 615, 788, 830, 891
MlyI GAGTC 4 cut(s) 261, 426, 560, 759
MmeI TCCRAC 1 cut(s) 289
Mph1103I ATGCAT 2 cut(s) 481, 919
MroXI GAANNNNTTC 1 cut(s) 792
MseI TTAA 5 cut(s) 12, 180, 297, 599, 1020
MslI CAYNNNNRTG 2 cut(s) 318, 659
MspA1I CMGCKG 3 cut(s) 31, 243, 785
MspI CCGG 1 cut(s) 411
Mva1269I GAATGC 1 cut(s) 460
MwoI GCNNNNNNNGC 3 cut(s) 62, 203, 243
NcoI CCATGG 1 cut(s) 313
NdeII GATC 4 cut(s) 151, 505, 875, 904
NlaIII CATG 6 cut(s) 191, 317, 341, 443, 866, 994
NlaIV GGNNCC 3 cut(s) 99, 275, 437
NmeAIII GCCGAG 1 cut(s) 72
NmuCI GTSAC 1 cut(s) 340
NsiI ATGCAT 2 cut(s) 481, 919
NspV TTCGAA 2 cut(s) 424, 643
PcsI WCGNNNNNNNCGW 1 cut(s) 421
PctI GAATGC 1 cut(s) 460
PdmI GAANNNNTTC 1 cut(s) 792
PfeI GAWTC 1 cut(s) 752
PkrI GCNGC 4 cut(s) 208, 245, 746, 842
PleI GAGTC 4 cut(s) 261, 425, 560, 759
PpsI GAGTC 4 cut(s) 261, 425, 560, 759
Ppu21I YACGTR 1 cut(s) 816
PshBI ATTAAT 1 cut(s) 12
PspN4I GGNNCC 3 cut(s) 99, 275, 437
PspPI GGNCC 2 cut(s) 97, 436
PstI CTGCAG 1 cut(s) 533
PsuI RGATCY 3 cut(s) 151, 875, 904
PvuII CAGCTG 2 cut(s) 31, 243
RsaI GTAC 2 cut(s) 177, 544
RsaNI GTAC 2 cut(s) 176, 543
RseI CAYNNNNRTG 2 cut(s) 318, 659
SaqAI TTAA 5 cut(s) 12, 180, 297, 599, 1020
SatI GCNGC 4 cut(s) 207, 244, 745, 841
Sau3AI GATC 4 cut(s) 151, 505, 875, 904
Sau96I GGNCC 2 cut(s) 97, 436
ScaI AGTACT 1 cut(s) 544
SchI GAGTC 4 cut(s) 261, 426, 560, 759
SduI GDGCHC 1 cut(s) 943
SfaNI GCATC 6 cut(s) 297, 416, 488, 847, 904, 926
SfcI CTRYAG 1 cut(s) 529
SfuI TTCGAA 2 cut(s) 424, 643
SmiMI CAYNNNNRTG 2 cut(s) 318, 659
SpeI ACTAGT 1 cut(s) 959
Sse9I AATT 9 cut(s) 13, 229, 359, 428, 561, 615, 788, 830, 891
SsiI CCGC 2 cut(s) 246, 783
SspMI CTAG 2 cut(s) 908, 960
StyI CCWWGG 2 cut(s) 313, 345
TaaI ACNGT 3 cut(s) 284, 640, 704
TaiI ACGT 1 cut(s) 818
TaqI TCGA 5 cut(s) 415, 424, 643, 755, 771
TaqII GACCGA 1 cut(s) 270
TasI AATT 9 cut(s) 13, 229, 359, 428, 561, 615, 788, 830, 891
TatI WGTACW 2 cut(s) 175, 542
TfiI GAWTC 1 cut(s) 752
Tru1I TTAA 5 cut(s) 12, 180, 297, 599, 1020
Tru9I TTAA 5 cut(s) 12, 180, 297, 599, 1020
TscAI CASTG 2 cut(s) 859, 957
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 4 cut(s) 206, 243, 744, 840
Tsp45I GTSAC 1 cut(s) 340
TspDTI ATGAA 1 cut(s) 990
TspGWI ACGGA 2 cut(s) 240, 317
TspRI CASTG 2 cut(s) 859, 957
VspI ATTAAT 1 cut(s) 12
XapI RAATTY 1 cut(s) 428
XmnI GAANNNNTTC 1 cut(s) 792
XspI CTAG 2 cut(s) 908, 960
ZrmI AGTACT 1 cut(s) 544
Zsp2I ATGCAT 2 cut(s) 481, 919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.