Rh6AG102200

WD repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0005405
Physical Location & Seq
Forward (+)
15180487 .. 15183710
3224 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG102200.1

Sequence Viewer

Length: 684 bp
ATGCTTCCACTTCTTCTTCATGCTCCAGAGAGAGCAGAGCCGTGGATTTCCGATCTTGGCACCGCTCTTCAGGCCTCGAAGCGACTTGTATTGGTGGCCCGTAGCTTCAATTCCAATTGTGACGCTCCTATGCTCGCCGTTATTGCTATTCTAGAACGGGAATTAAGAAAAATAATTGACTCGTTTACAGATAGTGTAATGTCTGTTTGTTTAACAAAGACTGAGATAATTGGTGGAAGTGTTGATGGTACTGTTCGAACATTTGACATTCGTATGGGAAGAGAGCTATCCGATGACTTGGGACAACCTGTGAACTGTATCTCAATGTCAAATGATGGTAACTGCATATTAGCAAGCTGCTTAGATTCGACTATACGACTCCTCGACAGGTCTTCCGCTGAATTACTTCAAGAATATAAAGGCCATACTTGTAAGTCTTACAAATTGGACTGCTGCCTTACCAACACTGATGCCCATGTAATCGGAGGATCTGAGGATGGTTCAATTTTCTTCTGGGATCTAGTAGATGCATCTGTTGTGTCAAGTATCCGAGCACATTCGTCAGTGGTAAAGCCTATTTTTCATTTACATGAATTGTGGTTTTCCTTTCGAATAGTTCTGACTGCTCGTGATATCCCTGCTACTTTGTGTTCCCAAGTATCCCCAAATAAAATACAGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

227

Amino Acids

24.99

Weight (kDa)

5.36

Isoelectric Point (pI)

44.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 52 - 191 4.4e-25 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 56 - 195 5.7e-12 CDC20/Fizzy WD40 domain
Beta-prop_ACSF4 PF13570 69 - 193 1.2e-06 Beta-alanine-activating enzyme, beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 72 - 188 5.8e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 72 - 191 6.7e-06 Echinoderm microtubule-associated protein second beta-propeller
WDR55 PF24796 73 - 193 3.9e-07 WDR55
Beta-prop_THOC3 PF25174 76 - 191 2.2e-10 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 76 - 193 2.7e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 101 - 196 2.2e-09 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 102 - 190 1.5e-07 WDHD1 first WD40 domain
Beta-prop_TEP1_2nd PF25047 103 - 198 4.3e-06 TEP-1 second beta-propeller
WD40_Prp19 PF24814 104 - 192 4.7e-09 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 104 - 193 3.1e-06 WDR3 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 59
AccBSI CCGCTC 1 cut(s) 65
AciI CCGC 2 cut(s) 63, 396
AclWI GGATC 2 cut(s) 496, 525
AcuI CTGAAG 1 cut(s) 53
AfaI GTAC 1 cut(s) 250
AgsI TTSAA 3 cut(s) 109, 410, 504
AluBI AGCT 3 cut(s) 105, 286, 357
AluI AGCT 3 cut(s) 105, 286, 357
Alw21I GWGCWC 1 cut(s) 556
AlwI GGATC 2 cut(s) 496, 525
AoxI GGCC 3 cut(s) 72, 96, 421
ApeKI GCWGC 2 cut(s) 357, 453
Asp700I GAANNNNTTC 1 cut(s) 405
AspS9I GGNCC 1 cut(s) 97
AsuII TTCGAA 2 cut(s) 256, 610
BanI GGYRCC 1 cut(s) 59
BauI CACGAG 1 cut(s) 627
BbsI GAAGAC 1 cut(s) 384
Bbv12I GWGCWC 1 cut(s) 556
BbvI GCAGC 2 cut(s) 344, 440
BccI CCATC 3 cut(s) 239, 329, 491
BceAI ACGGC 2 cut(s) 25, 122
BciVI GTATCC 2 cut(s) 557, 670
BfaI CTAG 2 cut(s) 152, 521
BfuI GTATCC 2 cut(s) 557, 670
BisI GCNGC 2 cut(s) 358, 454
BlsI GCNGC 2 cut(s) 359, 455
BmgT120I GGNCC 1 cut(s) 97
BmiI GGNNCC 1 cut(s) 61
BmsI GCATC 3 cut(s) 460, 517, 539
BpiI GAAGAC 1 cut(s) 384
BpmI CTGGAG 1 cut(s) 9
Bpu14I TTCGAA 2 cut(s) 256, 610
BsaJI CCNNGG 1 cut(s) 41
BseDI CCNNGG 1 cut(s) 41
BseGI GGATG 1 cut(s) 502
BseMII CTCAG 2 cut(s) 213, 483
BseRI GAGGAG 1 cut(s) 371
BseXI GCAGC 2 cut(s) 344, 440
BshFI GGCC 3 cut(s) 74, 98, 423
BshNI GGYRCC 1 cut(s) 59
BsiHKAI GWGCWC 1 cut(s) 556
BslFI GGGAC 1 cut(s) 315
BsmFI GGGAC 1 cut(s) 315
BsnI GGCC 3 cut(s) 74, 98, 423
Bsp119I TTCGAA 2 cut(s) 256, 610
Bsp1286I GDGCHC 1 cut(s) 556
Bsp143I GATC 3 cut(s) 52, 488, 517
BspACI CCGC 2 cut(s) 63, 396
BspANI GGCC 3 cut(s) 74, 98, 423
BspCNI CTCAG 2 cut(s) 214, 484
BspLI GGNNCC 1 cut(s) 61
BspPI GGATC 2 cut(s) 496, 525
BspQI GCTCTTC 1 cut(s) 72
BspT104I TTCGAA 2 cut(s) 256, 610
BspT107I GGYRCC 1 cut(s) 59
BsrBI CCGCTC 1 cut(s) 65
BssECI CCNNGG 1 cut(s) 41
BssMI GATC 3 cut(s) 52, 488, 517
BssSI CACGAG 1 cut(s) 627
Bst2BI CACGAG 1 cut(s) 627
Bst4CI ACNGT 2 cut(s) 253, 317
Bst6I CTCTTC 2 cut(s) 72, 274
BstBI TTCGAA 2 cut(s) 256, 610
BstC8I GCNNGC 2 cut(s) 135, 355
BstDEI CTNAG 3 cut(s) 222, 361, 492
BstDSI CCRYGG 1 cut(s) 41
BstF5I GGATG 1 cut(s) 502
BstKTI GATC 3 cut(s) 55, 491, 520
BstMBI GATC 3 cut(s) 52, 488, 517
BstMWI GCNNNNNNNGC 2 cut(s) 71, 143
BstV1I GCAGC 2 cut(s) 344, 440
BstV2I GAAGAC 1 cut(s) 384
BstX2I RGATCY 2 cut(s) 488, 517
BstYI RGATCY 2 cut(s) 488, 517
BsuI GTATCC 2 cut(s) 557, 670
BsuRI GGCC 3 cut(s) 74, 98, 423
BtgI CCRYGG 1 cut(s) 41
BtsCI GGATG 1 cut(s) 502
BtsIMutI CAGTG 2 cut(s) 465, 570
Cac8I GCNNGC 2 cut(s) 135, 355
Cfr13I GGNCC 1 cut(s) 97
CseI GACGC 1 cut(s) 131
Csp6I GTAC 1 cut(s) 249
CviAII CATG 3 cut(s) 20, 476, 590
CviJI RGCY 8 cut(s) 40, 74, 98, 105, 286, 357, 423, 574
CviKI_1 RGCY 8 cut(s) 40, 74, 98, 105, 286, 357, 423, 574
CviQI GTAC 1 cut(s) 249
DdeI CTNAG 3 cut(s) 222, 361, 492
DpnI GATC 3 cut(s) 54, 490, 519
DpnII GATC 3 cut(s) 52, 488, 517
Eam1104I CTCTTC 2 cut(s) 72, 274
EarI CTCTTC 2 cut(s) 72, 274
Eco147I AGGCCT 1 cut(s) 74
Eco32I GATATC 1 cut(s) 634
Eco57I CTGAAG 1 cut(s) 53
EcoRV GATATC 1 cut(s) 634
EcoT22I ATGCAT 1 cut(s) 532
FaeI CATG 3 cut(s) 23, 479, 593
FaiI YATR 9 cut(s) 21, 131, 275, 347, 374, 417, 426, 477, 591
FaqI GGGAC 1 cut(s) 315
FatI CATG 3 cut(s) 19, 475, 589
Fnu4HI GCNGC 2 cut(s) 358, 454
FokI GGATG 1 cut(s) 509
Fsp4HI GCNGC 2 cut(s) 358, 454
FspBI CTAG 2 cut(s) 152, 521
GluI GCNGC 2 cut(s) 358, 454
GsuI CTGGAG 1 cut(s) 9
HaeIII GGCC 3 cut(s) 74, 98, 423
HgaI GACGC 1 cut(s) 131
Hin1II CATG 3 cut(s) 23, 479, 593
HinfI GANTC 3 cut(s) 179, 365, 378
Hpy166II GTNNAC 2 cut(s) 186, 313
Hpy188I TCNGA 6 cut(s) 52, 292, 485, 493, 551, 621
Hpy188III TCNNGA 4 cut(s) 26, 152, 410, 629
Hpy8I GTNNAC 2 cut(s) 186, 313
HpyCH4III ACNGT 2 cut(s) 253, 317
HpyCH4V TGCA 2 cut(s) 345, 530
HpyF10VI GCNNNNNNNGC 2 cut(s) 71, 143
HpyF3I CTNAG 3 cut(s) 222, 361, 492
Hsp92II CATG 3 cut(s) 23, 479, 593
Kzo9I GATC 3 cut(s) 52, 488, 517
LguI GCTCTTC 1 cut(s) 72
LmnI GCTCC 2 cut(s) 28, 130
LpnPI CCDG 7 cut(s) 39, 56, 321, 373, 499, 651, 662
Lsp1109I GCAGC 2 cut(s) 344, 440
LweI GCATC 3 cut(s) 460, 517, 539
MaeI CTAG 2 cut(s) 152, 521
MaeIII GTNAC 2 cut(s) 119, 338
MalI GATC 3 cut(s) 54, 490, 519
MbiI CCGCTC 1 cut(s) 65
MboI GATC 3 cut(s) 52, 488, 517
MboII GAAGA 6 cut(s) 5, 8, 59, 291, 384, 502
MfeI CAATTG 1 cut(s) 115
MflI RGATCY 2 cut(s) 488, 517
MhlI GDGCHC 1 cut(s) 556
MluCI AATT 9 cut(s) 109, 115, 161, 174, 228, 401, 443, 504, 593
MlyI GAGTC 2 cut(s) 173, 372
MnlI CCTC 4 cut(s) 85, 392, 479, 487
Mph1103I ATGCAT 1 cut(s) 532
MroXI GAANNNNTTC 1 cut(s) 405
MseI TTAA 2 cut(s) 164, 212
MslI CAYNNNNRTG 2 cut(s) 272, 588
MspA1I CMGCKG 1 cut(s) 398
MunI CAATTG 1 cut(s) 115
MwoI GCNNNNNNNGC 2 cut(s) 71, 143
NdeII GATC 3 cut(s) 52, 488, 517
NlaIII CATG 3 cut(s) 23, 479, 593
NlaIV GGNNCC 1 cut(s) 61
NmuCI GTSAC 1 cut(s) 119
NsiI ATGCAT 1 cut(s) 532
NspV TTCGAA 2 cut(s) 256, 610
PceI AGGCCT 1 cut(s) 74
PciSI GCTCTTC 1 cut(s) 72
PdmI GAANNNNTTC 1 cut(s) 405
PfeI GAWTC 1 cut(s) 365
PkrI GCNGC 2 cut(s) 359, 455
PleI GAGTC 2 cut(s) 173, 372
PpsI GAGTC 2 cut(s) 173, 372
PspN4I GGNNCC 1 cut(s) 61
PspPI GGNCC 1 cut(s) 97
PsuI RGATCY 2 cut(s) 488, 517
RsaI GTAC 1 cut(s) 250
RsaNI GTAC 1 cut(s) 249
RseI CAYNNNNRTG 2 cut(s) 272, 588
SapI GCTCTTC 1 cut(s) 72
SaqAI TTAA 2 cut(s) 164, 212
SatI GCNGC 2 cut(s) 358, 454
Sau3AI GATC 3 cut(s) 52, 488, 517
Sau96I GGNCC 1 cut(s) 97
SchI GAGTC 2 cut(s) 173, 372
SduI GDGCHC 1 cut(s) 556
SetI ASST 5 cut(s) 107, 288, 310, 359, 392
SfaNI GCATC 3 cut(s) 460, 517, 539
SfuI TTCGAA 2 cut(s) 256, 610
SmiMI CAYNNNNRTG 2 cut(s) 272, 588
Sse9I AATT 9 cut(s) 109, 115, 161, 174, 228, 401, 443, 504, 593
SseBI AGGCCT 1 cut(s) 74
SsiI CCGC 2 cut(s) 63, 396
SspMI CTAG 2 cut(s) 152, 521
StuI AGGCCT 1 cut(s) 74
TaaI ACNGT 2 cut(s) 253, 317
TaqI TCGA 5 cut(s) 77, 256, 368, 384, 610
TasI AATT 9 cut(s) 109, 115, 161, 174, 228, 401, 443, 504, 593
TfiI GAWTC 1 cut(s) 365
Tru1I TTAA 2 cut(s) 164, 212
Tru9I TTAA 2 cut(s) 164, 212
TscAI CASTG 2 cut(s) 472, 570
TseFI GTSAC 1 cut(s) 119
TseI GCWGC 2 cut(s) 357, 453
Tsp45I GTSAC 1 cut(s) 119
TspDTI ATGAA 3 cut(s) 8, 572, 606
TspRI CASTG 2 cut(s) 472, 570
XbaI TCTAGA 1 cut(s) 151
XmnI GAANNNNTTC 1 cut(s) 405
XspI CTAG 2 cut(s) 152, 521
Zsp2I ATGCAT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.