FvH4_1g23870

WD repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0005405
Physical Location & Seq
Forward (+)
15721196 .. 15725366
4171 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g23870.t1

Sequence Viewer

Length: 900 bp
ATGAGTGTGTCAGATCTGCCAAAGAAGGAGGGAAATGTACTTAAAGGGCATGAGGGAGCAGTATTAGCAGCGAGGTTCAATAAAGATGGAAATTATTGCCTCAGCTGCGGGAAAGACCGAACCATTCGACTTTGGAACCCTCACCGTGGCATCCACATTAAAACCTACAAGTCCCATGGCCGTGAAGTCCGTGATGTCCATGTCACCTCGGACAATTCCAAATTATGTTCAGGTGGTGGTGACAGACAAATATTCTATTGGGATGTGGCATCCGGTCGAGTCATTCGAAAATTTCGGGGCCATGATGGAGGGGTGAATGCTGTGAAGTTTAATGAGTATGCATCTGTTGTAGTCTCAGCTGGATATGATCAGTCATTGCGTGCTTGGGATTGCAGATCTCACAGTACTGAGCCTATTCAGATAATTGACTCATTTACAGATAGTGTAATGTCGATTTGTTTAACAAAGACTGAGATTATTGGTGGAAGTGTTGATGGAACTGTTCGAACATTTGACATTCGTATGGGAAGAGAACTATCCGATGACTTGGGACAACCTGTGAACTGTATATCTATGTCAAATGATGGTAACTGCATATTAGCAAGCTGCTTAGATTCAACGATACGTCTCCTCGACAGGACTTCTGCTGAATTACTTCAAGAATATAAAGGCCATACTTGTAAGTCTTACAAATTGGACTGCTGCCTTACCAACTCTGATGCCCATGTAATTGGAGGATCTGAGGATGGTTCAATCTTCTTCTGGGATCTAGTAGATGCATCTGTTGTATCCAGTATCCGGGCACATTTGTCAGTGGTAACGAGTGTCAGTTATCACCCTAAGGACAACTGCATGATTAGTTCTTCCGTAGATGGCACCATTAAGGTGTGGAAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

300

Amino Acids

32.87

Weight (kDa)

6.82

Isoelectric Point (pI)

35.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 4 - 82 1.2e-12 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 4 - 135 6.8e-13 G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd PF25047 10 - 132 1.5e-13 TEP-1 second beta-propeller
WDR55 PF24796 10 - 180 9.8e-14 WDR55
WD40_CDC20-Fz PF24807 12 - 94 2e-08 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 12 - 68 9.3e-07 Prp19 WD40 domain
WD40 PF00400 12 - 46 3.2e-09 WD domain, G-beta repeat
EIF3I PF24805 13 - 225 3.8e-24 EIF3I
Beta-prop_WDR5 PF25175 14 - 298 3.5e-65 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 14 - 89 8.8e-09 WDR3 second beta-propeller domain
Beta-prop_EML PF23409 15 - 158 4.2e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 15 - 146 6.2e-12 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 17 - 96 3.6e-13 THOC3 beta-propeller domain
Beta-prop_SCAP PF24017 37 - 132 6.6e-07 SCAP Beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 40 - 129 1.6e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 41 - 173 2.3e-21 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 51 - 135 2.5e-07 CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz PF24807 53 - 134 3e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR41 PF25178 53 - 155 2.5e-06 WDR41
Beta-prop_THOC3 PF25174 59 - 299 7.5e-31 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 61 - 172 9.9e-11 WDR3 second beta-propeller domain
WD40_WDHD1_1st PF24817 62 - 137 3.1e-08 WDHD1 first WD40 domain
WD40_Prp19 PF24814 70 - 196 3.1e-16 Prp19 WD40 domain
WD40 PF00400 93 - 130 1.6e-06 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 97 - 184 1e-06 TEP-1 second beta-propeller
WD40_MABP1-WDR62_2nd PF24782 97 - 257 3e-07 MABP1/WDR62 second WD40 domain
Beta-prop_EML_2 PF23414 112 - 228 5.3e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 145 - 266 1.5e-08 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 155 - 291 1.6e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 156 - 298 9.7e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_ACSF4 PF13570 158 - 296 9.7e-08 Beta-alanine-activating enzyme, beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 159 - 297 9.6e-12 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 184 - 298 4.5e-11 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 185 - 298 1.6e-09 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 185 - 298 5.7e-18 WDR3 first beta-propeller domain
WDR55 PF24796 186 - 298 6.6e-11 WDR55
Beta-prop_WDR3_2nd PF25172 187 - 298 1.2e-11 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 187 - 297 1e-15 Prp19 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 192 - 298 4.5e-08 MABP1/WDR62 second WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 223 - 298 7.9e-06 CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st PF24817 235 - 298 7.3e-10 WDHD1 first WD40 domain
WD40 PF00400 262 - 297 2.1e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 875
AciI CCGC 1 cut(s) 108
AclWI GGATC 2 cut(s) 745, 774
AcoI YGGCCR 1 cut(s) 178
AcsI RAATTY 1 cut(s) 290
AfaI GTAC 2 cut(s) 39, 406
AfiI CCNNNNNNNGG 2 cut(s) 146, 798
AgsI TTSAA 4 cut(s) 79, 618, 659, 753
AluBI AGCT 3 cut(s) 105, 359, 606
AluI AGCT 3 cut(s) 105, 359, 606
Alw26I GTCTC 2 cut(s) 358, 632
AlwI GGATC 2 cut(s) 745, 774
AoxI GGCC 3 cut(s) 178, 298, 670
ApeKI GCWGC 4 cut(s) 68, 105, 606, 702
ApoI RAATTY 1 cut(s) 290
Asp700I GAANNNNTTC 1 cut(s) 654
AspS9I GGNCC 1 cut(s) 298
AsuC2I CCSGG 1 cut(s) 800
AsuHPI GGTGA 5 cut(s) 134, 196, 251, 325, 827
AsuII TTCGAA 2 cut(s) 286, 505
AxyI CCTNAGG 1 cut(s) 840
BaeGI GKGCMC 1 cut(s) 805
BanI GGYRCC 1 cut(s) 875
BbvCI CCTCAGC 1 cut(s) 101
BbvI GCAGC 4 cut(s) 80, 92, 593, 689
BccI CCATC 6 cut(s) 80, 299, 488, 578, 740, 866
BceAI ACGGC 1 cut(s) 165
BciVI GTATCC 2 cut(s) 799, 806
BclI TGATCA 1 cut(s) 367
BcnI CCSGG 1 cut(s) 800
BcoDI GTCTC 2 cut(s) 358, 632
BfaI CTAG 1 cut(s) 770
BfuI GTATCC 2 cut(s) 799, 806
BglII AGATCT 2 cut(s) 13, 395
BisI GCNGC 4 cut(s) 69, 106, 607, 703
BlsI GCNGC 4 cut(s) 70, 107, 608, 704
BmcAI AGTACT 1 cut(s) 406
Bme1390I CCNGG 1 cut(s) 800
BmgT120I GGNCC 1 cut(s) 298
BmiI GGNNCC 3 cut(s) 137, 299, 877
BmrFI CCNGG 1 cut(s) 800
BmsI GCATC 6 cut(s) 159, 278, 350, 709, 766, 788
Bpu10I CCTNAGC 1 cut(s) 101
Bpu14I TTCGAA 2 cut(s) 286, 505
BpuMI CCSGG 1 cut(s) 800
BsaJI CCNNGG 3 cut(s) 145, 175, 207
BsaWI WCCGGW 1 cut(s) 272
Bsc4I CCNNNNNNNGG 2 cut(s) 146, 798
Bse1I ACTGG 1 cut(s) 792
Bse21I CCTNAGG 1 cut(s) 840
Bse3DI GCAATG 1 cut(s) 374
BseDI CCNNGG 3 cut(s) 145, 175, 207
BseGI GGATG 4 cut(s) 150, 268, 269, 751
BseLI CCNNNNNNNGG 2 cut(s) 146, 798
BseMI GCAATG 1 cut(s) 374
BseMII CTCAG 5 cut(s) 115, 369, 399, 462, 732
BseNI ACTGG 1 cut(s) 792
BseRI GAGGAG 1 cut(s) 620
BseSI GKGCMC 1 cut(s) 805
BseXI GCAGC 4 cut(s) 80, 92, 593, 689
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 3 cut(s) 180, 300, 672
BshNI GGYRCC 1 cut(s) 875
BsiEI CGRYCG 1 cut(s) 277
BsiSI CCGG 2 cut(s) 273, 799
BslFI GGGAC 2 cut(s) 157, 564
BslI CCNNNNNNNGG 2 cut(s) 146, 798
BsmAI GTCTC 2 cut(s) 358, 632
BsmBI CGTCTC 1 cut(s) 632
BsmFI GGGAC 2 cut(s) 157, 564
BsmI GAATGC 1 cut(s) 322
BsnI GGCC 3 cut(s) 180, 300, 672
Bsp119I TTCGAA 2 cut(s) 286, 505
Bsp1286I GDGCHC 1 cut(s) 805
Bsp143I GATC 5 cut(s) 13, 367, 395, 737, 766
Bsp19I CCATGG 1 cut(s) 175
BspACI CCGC 1 cut(s) 108
BspANI GGCC 3 cut(s) 180, 300, 672
BspCNI CTCAG 5 cut(s) 114, 368, 400, 463, 733
BspLI GGNNCC 3 cut(s) 137, 299, 877
BspPI GGATC 2 cut(s) 745, 774
BspT104I TTCGAA 2 cut(s) 286, 505
BspT107I GGYRCC 1 cut(s) 875
BsrDI GCAATG 1 cut(s) 374
BsrI ACTGG 1 cut(s) 792
BssECI CCNNGG 3 cut(s) 145, 175, 207
BssMI GATC 5 cut(s) 13, 367, 395, 737, 766
BssT1I CCWWGG 1 cut(s) 175
Bst4CI ACNGT 4 cut(s) 146, 404, 502, 566
Bst6I CTCTTC 1 cut(s) 523
BstBI TTCGAA 2 cut(s) 286, 505
BstC8I GCNNGC 2 cut(s) 381, 604
BstDEI CTNAG 7 cut(s) 101, 355, 408, 471, 610, 741, 840
BstDSI CCRYGG 2 cut(s) 145, 175
BstF5I GGATG 4 cut(s) 150, 268, 269, 751
BstKTI GATC 5 cut(s) 16, 370, 398, 740, 769
BstMAI GTCTC 2 cut(s) 358, 632
BstMBI GATC 5 cut(s) 13, 367, 395, 737, 766
BstMCI CGRYCG 1 cut(s) 277
BstMWI GCNNNNNNNGC 2 cut(s) 65, 105
BstSCI CCNGG 1 cut(s) 798
BstSLI GKGCMC 1 cut(s) 805
BstV1I GCAGC 4 cut(s) 80, 92, 593, 689
BstX2I RGATCY 4 cut(s) 13, 395, 737, 766
BstXI CCANNNNNNTGG 1 cut(s) 731
BstYI RGATCY 4 cut(s) 13, 395, 737, 766
Bsu36I CCTNAGG 1 cut(s) 840
BsuI GTATCC 2 cut(s) 799, 806
BsuRI GGCC 3 cut(s) 180, 300, 672
BtgI CCRYGG 2 cut(s) 145, 175
BtsCI GGATG 4 cut(s) 150, 268, 269, 751
BtsIMutI CAGTG 1 cut(s) 819
Cac8I GCNNGC 2 cut(s) 381, 604
Cfr13I GGNCC 1 cut(s) 298
Csp6I GTAC 2 cut(s) 38, 405
CviAII CATG 6 cut(s) 50, 176, 200, 302, 725, 853
CviJI RGCY 7 cut(s) 105, 180, 300, 359, 412, 606, 672
CviKI_1 RGCY 7 cut(s) 105, 180, 300, 359, 412, 606, 672
CviQI GTAC 2 cut(s) 38, 405
DdeI CTNAG 7 cut(s) 101, 355, 408, 471, 610, 741, 840
DpnI GATC 5 cut(s) 15, 369, 397, 739, 768
DpnII GATC 5 cut(s) 13, 367, 395, 737, 766
EaeI YGGCCR 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 523
EarI CTCTTC 1 cut(s) 523
Eco130I CCWWGG 1 cut(s) 175
Eco81I CCTNAGG 1 cut(s) 840
EcoT14I CCWWGG 1 cut(s) 175
EcoT22I ATGCAT 2 cut(s) 343, 781
ErhI CCWWGG 1 cut(s) 175
Esp3I CGTCTC 1 cut(s) 632
FaeI CATG 6 cut(s) 53, 179, 203, 305, 728, 856
FaqI GGGAC 2 cut(s) 157, 564
FatI CATG 6 cut(s) 49, 175, 199, 301, 724, 852
FauI CCCGC 1 cut(s) 101
FbaI TGATCA 1 cut(s) 367
Fnu4HI GCNGC 4 cut(s) 69, 106, 607, 703
FokI GGATG 4 cut(s) 137, 256, 275, 758
Fsp4HI GCNGC 4 cut(s) 69, 106, 607, 703
FspBI CTAG 1 cut(s) 770
GluI GCNGC 4 cut(s) 69, 106, 607, 703
HaeIII GGCC 3 cut(s) 180, 300, 672
HapII CCGG 2 cut(s) 273, 799
Hin1II CATG 6 cut(s) 53, 179, 203, 305, 728, 856
HinfI GANTC 3 cut(s) 279, 428, 614
HpaII CCGG 2 cut(s) 273, 799
HphI GGTGA 5 cut(s) 134, 196, 251, 325, 827
Hpy166II GTNNAC 1 cut(s) 562
Hpy188I TCNGA 6 cut(s) 13, 211, 420, 541, 718, 742
Hpy188III TCNNGA 1 cut(s) 659
Hpy8I GTNNAC 1 cut(s) 562
HpyAV CCTTC 1 cut(s) 19
HpyCH4III ACNGT 4 cut(s) 146, 404, 502, 566
HpyCH4IV ACGT 1 cut(s) 625
HpyCH4V TGCA 5 cut(s) 341, 393, 594, 779, 852
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 105
HpyF3I CTNAG 7 cut(s) 101, 355, 408, 471, 610, 741, 840
HpySE526I ACGT 1 cut(s) 625
Hsp92II CATG 6 cut(s) 53, 179, 203, 305, 728, 856
Ksp22I TGATCA 1 cut(s) 367
Kzo9I GATC 5 cut(s) 13, 367, 395, 737, 766
LmnI GCTCC 1 cut(s) 56
LpnPI CCDG 8 cut(s) 216, 286, 345, 570, 622, 748, 805, 812
Lsp1109I GCAGC 4 cut(s) 80, 92, 593, 689
LweI GCATC 6 cut(s) 159, 278, 350, 709, 766, 788
MaeI CTAG 1 cut(s) 770
MaeII ACGT 1 cut(s) 625
MaeIII GTNAC 4 cut(s) 202, 239, 587, 817
MalI GATC 5 cut(s) 15, 369, 397, 739, 768
MboI GATC 5 cut(s) 13, 367, 395, 737, 766
MboII GAAGA 4 cut(s) 540, 748, 751, 855
MflI RGATCY 4 cut(s) 13, 395, 737, 766
MhlI GDGCHC 1 cut(s) 805
MluCI AATT 8 cut(s) 91, 214, 221, 290, 423, 650, 692, 729
MlyI GAGTC 2 cut(s) 288, 422
Mph1103I ATGCAT 2 cut(s) 343, 781
MroXI GAANNNNTTC 1 cut(s) 654
MseI TTAA 5 cut(s) 42, 159, 330, 461, 882
MslI CAYNNNNRTG 3 cut(s) 180, 521, 884
MspA1I CMGCKG 2 cut(s) 105, 359
MspI CCGG 2 cut(s) 273, 799
MspR9I CCNGG 1 cut(s) 800
Mva1269I GAATGC 1 cut(s) 322
MwoI GCNNNNNNNGC 2 cut(s) 65, 105
NciI CCSGG 1 cut(s) 800
NcoI CCATGG 1 cut(s) 175
NdeII GATC 5 cut(s) 13, 367, 395, 737, 766
NlaIII CATG 6 cut(s) 53, 179, 203, 305, 728, 856
NlaIV GGNNCC 3 cut(s) 137, 299, 877
NmuCI GTSAC 2 cut(s) 202, 239
NsiI ATGCAT 2 cut(s) 343, 781
NspV TTCGAA 2 cut(s) 286, 505
PcsI WCGNNNNNNNCGW 1 cut(s) 283
PctI GAATGC 1 cut(s) 322
PdmI GAANNNNTTC 1 cut(s) 654
PfeI GAWTC 1 cut(s) 614
PkrI GCNGC 4 cut(s) 70, 107, 608, 704
PleI GAGTC 2 cut(s) 287, 422
PpsI GAGTC 2 cut(s) 287, 422
PspN4I GGNNCC 3 cut(s) 137, 299, 877
PspPI GGNCC 1 cut(s) 298
PsuI RGATCY 4 cut(s) 13, 395, 737, 766
PvuII CAGCTG 2 cut(s) 105, 359
RsaI GTAC 2 cut(s) 39, 406
RsaNI GTAC 2 cut(s) 38, 405
RseI CAYNNNNRTG 3 cut(s) 180, 521, 884
SaqAI TTAA 5 cut(s) 42, 159, 330, 461, 882
SatI GCNGC 4 cut(s) 69, 106, 607, 703
Sau3AI GATC 5 cut(s) 13, 367, 395, 737, 766
Sau96I GGNCC 1 cut(s) 298
ScaI AGTACT 1 cut(s) 406
SchI GAGTC 2 cut(s) 288, 422
ScrFI CCNGG 1 cut(s) 800
SduI GDGCHC 1 cut(s) 805
SfaNI GCATC 6 cut(s) 159, 278, 350, 709, 766, 788
SfuI TTCGAA 2 cut(s) 286, 505
SmiMI CAYNNNNRTG 3 cut(s) 180, 521, 884
Sse9I AATT 8 cut(s) 91, 214, 221, 290, 423, 650, 692, 729
SsiI CCGC 1 cut(s) 108
SspI AATATT 1 cut(s) 252
SspMI CTAG 1 cut(s) 770
StyD4I CCNGG 1 cut(s) 798
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 4 cut(s) 146, 404, 502, 566
TaiI ACGT 1 cut(s) 628
TaqI TCGA 6 cut(s) 127, 277, 286, 452, 505, 633
TaqII GACCGA 1 cut(s) 132
TasI AATT 8 cut(s) 91, 214, 221, 290, 423, 650, 692, 729
TatI WGTACW 2 cut(s) 37, 404
TfiI GAWTC 1 cut(s) 614
Tru1I TTAA 5 cut(s) 42, 159, 330, 461, 882
Tru9I TTAA 5 cut(s) 42, 159, 330, 461, 882
TscAI CASTG 1 cut(s) 819
TseFI GTSAC 2 cut(s) 202, 239
TseI GCWGC 4 cut(s) 68, 105, 606, 702
Tsp45I GTSAC 2 cut(s) 202, 239
TspGWI ACGGA 2 cut(s) 179, 856
TspRI CASTG 1 cut(s) 819
XapI RAATTY 1 cut(s) 290
XmnI GAANNNNTTC 1 cut(s) 654
XspI CTAG 1 cut(s) 770
ZrmI AGTACT 1 cut(s) 406
Zsp2I ATGCAT 2 cut(s) 343, 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.