AT5G65180

RPR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010009
Physical Location & Seq
Forward (+)
26045327 .. 26048031
2705 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G65180.1

Sequence Viewer

Length: 1320 bp
ATGAGTAGTCCGTTCAGTGAAGAGATACTGATTGATAACCTCGCTAAGCTCAACAGCACTCAACAATCCATCCAAACTTTGTCACAGTGGTGTATAGTCCATCGTAGTGAAGCTGAGCTGGTTGTTACGACATGGGAGAAACAGTTTCACAGTACACAGATAGGTCAGAAAGTGCCTCTCTTGTACCTTGCTAATGACATTCTTCAGAACAGCAAGCGTCAGGGTAATGAGTTTGTGCAAGAGTTCTGGAAAGTTCTCCCTGGTGCTCTCAAAGATATTGTTTCCTTGGGAGATGACTATGGAAAAGGCGTGGTTTCGCGATTGGTTAATATATGGGAAGAGAGAAGAGTCTTTGGATCCCGTTCAAAGAGTCTTAAAGATGTAATGCTCTCAGAAGAAGCTCCACCACCACTTGATGTCAGTAAAAAGCGATTTCGGGGATCTAAATCTGCAAAACGAGATTCAAAATCTACCAAAACGAAATTATCAAGTGGAGGTGTGTCCGAGAAGATAGTATCTGCATTCAATTTGGTTCGTGCAGAGAACTCAAATGAAGAGACAGAGATGAACAAATGCAAGTCTGCCGTTAGACGTATCAGAAAGATGGAGAAAGATGTTGAAGATGCTTGCTCCACTGCAAAAGACCCGAGGAAAGAATCATTGGCAAAAGAATTGGAAGAGGAAGAAAACATTCTGAGACAATCTGTTGAGAAACTTAAATCTGTTGAAGAAAGTAGGACATCTCTCGTAAATCATCTTAGGGAAGCGCTACGGGAACAGGAATCTGAATTGGAGAATCTTCAATCTCAGATACAGGTAGCACAAGAACAAACCGAAGAAGCCCAAAACATGCAGAAACGGCTCAATAACGAAACACCGGTTAACAATAACAACGGAACATCTGGTCAATCAGCGAAAATCACTCCTGCCTCAATCGCTGCAATGGCGGAAATGCTAACCTCATCAACAAACTCATCAATGATTATGCATTCTGTCCTCTCTTCATTTGCAGCCGAAGCTACACAAACTTCTGGTCTAACCAAATCAAACACCTCAGACACAAATGCATTTGTTGTTCCTCCAAATCCTCAGCAGTATCACATAATACCAAATCCAGCCGCATCACAACAGTTTCCTTATGGATTTGGTAACATTCCATTAATGCCACCTGGAGCACTGCCGCCACCGCCGCCTGGAACGTTGCCGCCGCATATGATGAATAATAACAACCAACCAAATGCAGCTCAGCAACAAGCGCAACAAGGTCAGAGTTTTCATCCACCGGGTATGATGTATTTTGGACCTCCACACCATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CTD_bind

Protein Analysis

439

Amino Acids

48.71

Weight (kDa)

6.54

Isoelectric Point (pI)

63.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CID PF04818 9 - 120 4.3e-35 CID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 319
AciI CCGC 7 cut(s) 947, 1119, 1181, 1187, 1190, 1205, 1208
AclI AACGTT 1 cut(s) 1199
AclWI GGATC 3 cut(s) 351, 364, 448
AcuI CTGAAG 1 cut(s) 188
AfaI GTAC 2 cut(s) 154, 185
AfeI AGCGCT 1 cut(s) 768
AfiI CCNNNNNNNGG 1 cut(s) 1193
AgeI ACCGGT 1 cut(s) 877
AgsI TTSAA 6 cut(s) 366, 465, 526, 620, 728, 803
AjnI CCWGG 3 cut(s) 259, 1168, 1192
AjuI GAANNNNNNNTTGG 2 cut(s) 644, 676
AleI CACNNNNGTG 1 cut(s) 88
AluBI AGCT 6 cut(s) 49, 113, 118, 401, 1019, 1244
AluI AGCT 6 cut(s) 49, 113, 118, 401, 1019, 1244
Alw21I GWGCWC 2 cut(s) 268, 1177
Alw26I GTCTC 2 cut(s) 551, 691
AlwI GGATC 3 cut(s) 351, 364, 448
Ama87I CYCGRG 1 cut(s) 646
Aor51HI AGCGCT 1 cut(s) 768
ApeKI GCWGC 3 cut(s) 938, 1010, 1241
ArsI GACNNNNNNTTYG 2 cut(s) 1018, 1050
AseI ATTAAT 1 cut(s) 1160
AsiGI ACCGGT 1 cut(s) 877
Asp700I GAANNNNTTC 1 cut(s) 690
AspLEI GCGC 2 cut(s) 769, 1258
AspS9I GGNCC 1 cut(s) 1301
AsuC2I CCSGG 1 cut(s) 1284
AvaI CYCGRG 1 cut(s) 646
AvaII GGWCC 1 cut(s) 1301
BamHI GGATCC 1 cut(s) 356
Bbv12I GWGCWC 2 cut(s) 268, 1177
BbvCI CCTCAGC 1 cut(s) 1089
BbvI GCAGC 3 cut(s) 925, 1022, 1253
BccI CCATC 3 cut(s) 77, 108, 598
BceAI ACGGC 2 cut(s) 569, 875
BciT130I CCWGG 3 cut(s) 261, 1170, 1194
BcnI CCSGG 1 cut(s) 1284
BcoDI GTCTC 2 cut(s) 551, 691
BfoI RGCGCY 1 cut(s) 770
BisI GCNGC 8 cut(s) 939, 1011, 1119, 1181, 1190, 1205, 1208, 1242
BlpI GCTNAGC 3 cut(s) 45, 114, 1245
BlsI GCNGC 8 cut(s) 940, 1012, 1120, 1182, 1191, 1206, 1209, 1243
Bme1390I CCNGG 4 cut(s) 261, 1170, 1194, 1284
Bme18I GGWCC 1 cut(s) 1301
BmeT110I CYCGRG 1 cut(s) 646
BmgT120I GGNCC 1 cut(s) 1301
BmiI GGNNCC 1 cut(s) 358
BmrFI CCNGG 4 cut(s) 261, 1170, 1194, 1284
BmsI GCATC 2 cut(s) 613, 1130
BpmI CTGGAG 1 cut(s) 1191
Bpu10I CCTNAGC 1 cut(s) 1089
Bpu1102I GCTNAGC 3 cut(s) 45, 114, 1245
BpuMI CCSGG 1 cut(s) 1284
BsaBI GATNNNNATC 1 cut(s) 445
BsaJI CCNNGG 3 cut(s) 259, 285, 647
BsaWI WCCGGW 1 cut(s) 877
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bsc4I CCNNNNNNNGG 1 cut(s) 1193
Bse118I RCCGGY 1 cut(s) 877
Bse3DI GCAATG 1 cut(s) 948
Bse8I GATNNNNATC 1 cut(s) 445
BseBI CCWGG 3 cut(s) 261, 1170, 1194
BseDI CCNNGG 3 cut(s) 259, 285, 647
BseGI GGATG 2 cut(s) 69, 1276
BseJI GATNNNNATC 1 cut(s) 445
BseLI CCNNNNNNNGG 1 cut(s) 1193
BseMI GCAATG 1 cut(s) 948
BseMII CTCAG 7 cut(s) 105, 405, 686, 821, 1068, 1103, 1259
BseXI GCAGC 3 cut(s) 925, 1022, 1253
BsgI GTGCAG 1 cut(s) 558
Bsh1236I CGCG 1 cut(s) 319
BshTI ACCGGT 1 cut(s) 877
BsiHKAI GWGCWC 2 cut(s) 268, 1177
BsiHKCI CYCGRG 1 cut(s) 646
BsiSI CCGG 2 cut(s) 878, 1283
BslI CCNNNNNNNGG 1 cut(s) 1193
BsmAI GTCTC 2 cut(s) 551, 691
BsmI GAATGC 2 cut(s) 521, 988
BsoBI CYCGRG 1 cut(s) 646
Bsp1286I GDGCHC 2 cut(s) 268, 1177
Bsp143I GATC 2 cut(s) 356, 440
Bsp1720I GCTNAGC 3 cut(s) 45, 114, 1245
Bsp68I TCGCGA 1 cut(s) 319
BspACI CCGC 7 cut(s) 947, 1119, 1181, 1187, 1190, 1205, 1208
BspCNI CTCAG 7 cut(s) 106, 404, 687, 820, 1067, 1102, 1258
BspFNI CGCG 1 cut(s) 319
BspLI GGNNCC 1 cut(s) 358
BspPI GGATC 3 cut(s) 351, 364, 448
BsrDI GCAATG 1 cut(s) 948
BsrFI RCCGGY 1 cut(s) 877
BssAI RCCGGY 1 cut(s) 877
BssECI CCNNGG 3 cut(s) 259, 285, 647
BssMI GATC 2 cut(s) 356, 440
BssT1I CCWWGG 1 cut(s) 285
Bst2UI CCWGG 3 cut(s) 261, 1170, 1194
Bst4CI ACNGT 4 cut(s) 87, 144, 152, 1131
Bst6I CTCTTC 6 cut(s) 15, 333, 340, 549, 672, 1006
BstC8I GCNNGC 2 cut(s) 215, 628
BstDEI CTNAG 9 cut(s) 45, 114, 391, 695, 758, 807, 1054, 1089, 1245
BstF5I GGATG 2 cut(s) 69, 1276
BstFNI CGCG 1 cut(s) 319
BstH2I RGCGCY 1 cut(s) 770
BstHHI GCGC 2 cut(s) 769, 1258
BstKTI GATC 2 cut(s) 359, 443
BstMAI GTCTC 2 cut(s) 551, 691
BstMBI GATC 2 cut(s) 356, 440
BstMWI GCNNNNNNNGC 7 cut(s) 859, 935, 944, 1016, 1186, 1189, 1255
BstNI CCWGG 3 cut(s) 261, 1170, 1194
BstNSI RCATGY 1 cut(s) 853
BstSCI CCNGG 4 cut(s) 259, 1168, 1192, 1282
BstUI CGCG 1 cut(s) 319
BstV1I GCAGC 3 cut(s) 925, 1022, 1253
BstX2I RGATCY 2 cut(s) 356, 440
BstYI RGATCY 2 cut(s) 356, 440
BtsCI GGATG 2 cut(s) 69, 1276
BtsI GCAGTG 2 cut(s) 633, 1175
BtsIMutI CAGTG 4 cut(s) 22, 92, 633, 1175
BtuMI TCGCGA 1 cut(s) 319
Cac8I GCNNGC 2 cut(s) 215, 628
CfoI GCGC 2 cut(s) 769, 1258
Cfr10I RCCGGY 1 cut(s) 877
Cfr13I GGNCC 1 cut(s) 1301
CseI GACGC 1 cut(s) 206
Csp6I GTAC 2 cut(s) 153, 184
CspAI ACCGGT 1 cut(s) 877
CviAII CATG 2 cut(s) 132, 850
CviQI GTAC 2 cut(s) 153, 184
DdeI CTNAG 9 cut(s) 45, 114, 391, 695, 758, 807, 1054, 1089, 1245
DpnI GATC 2 cut(s) 358, 442
DpnII GATC 2 cut(s) 356, 440
Eam1104I CTCTTC 6 cut(s) 15, 333, 340, 549, 672, 1006
EarI CTCTTC 6 cut(s) 15, 333, 340, 549, 672, 1006
EciI GGCGGA 1 cut(s) 962
Eco130I CCWWGG 1 cut(s) 285
Eco47I GGWCC 1 cut(s) 1301
Eco47III AGCGCT 1 cut(s) 768
Eco57I CTGAAG 1 cut(s) 188
Eco88I CYCGRG 1 cut(s) 646
EcoRII CCWGG 3 cut(s) 259, 1168, 1192
EcoT14I CCWWGG 1 cut(s) 285
EcoT22I ATGCAT 2 cut(s) 990, 1069
ErhI CCWWGG 1 cut(s) 285
FaeI CATG 2 cut(s) 135, 853
FatI CATG 2 cut(s) 131, 849
FauNDI CATATG 1 cut(s) 1212
Fnu4HI GCNGC 8 cut(s) 939, 1011, 1119, 1181, 1190, 1205, 1208, 1242
FokI GGATG 2 cut(s) 56, 1263
Fsp4HI GCNGC 8 cut(s) 939, 1011, 1119, 1181, 1190, 1205, 1208, 1242
GlaI GCGC 2 cut(s) 768, 1257
GluI GCNGC 8 cut(s) 939, 1011, 1119, 1181, 1190, 1205, 1208, 1242
GsuI CTGGAG 1 cut(s) 1191
HaeII RGCGCY 1 cut(s) 770
HapII CCGG 2 cut(s) 878, 1283
HgaI GACGC 1 cut(s) 206
HhaI GCGC 2 cut(s) 769, 1258
Hin1II CATG 2 cut(s) 135, 853
Hin6I GCGC 2 cut(s) 767, 1256
HinP1I GCGC 2 cut(s) 767, 1256
HincII GTYRAC 1 cut(s) 883
HindII GTYRAC 1 cut(s) 883
HinfI GANTC 6 cut(s) 348, 370, 461, 656, 782, 796
HpaI GTTAAC 1 cut(s) 883
HpaII CCGG 2 cut(s) 878, 1283
Hpy166II GTNNAC 2 cut(s) 155, 883
Hpy188III TCNNGA 2 cut(s) 247, 318
Hpy8I GTNNAC 2 cut(s) 155, 883
HpyCH4III ACNGT 4 cut(s) 87, 144, 152, 1131
HpyCH4IV ACGT 2 cut(s) 592, 1199
HpyF10VI GCNNNNNNNGC 7 cut(s) 859, 935, 944, 1016, 1186, 1189, 1255
HpyF3I CTNAG 9 cut(s) 45, 114, 391, 695, 758, 807, 1054, 1089, 1245
HpySE526I ACGT 2 cut(s) 592, 1199
Hsp92II CATG 2 cut(s) 135, 853
HspAI GCGC 2 cut(s) 767, 1256
KspAI GTTAAC 1 cut(s) 883
Kzo9I GATC 2 cut(s) 356, 440
LmnI GCTCC 3 cut(s) 406, 635, 1172
Lsp1109I GCAGC 3 cut(s) 925, 1022, 1253
LweI GCATC 2 cut(s) 613, 1130
MaeII ACGT 2 cut(s) 592, 1199
MaeIII GTNAC 3 cut(s) 81, 124, 1148
MalI GATC 2 cut(s) 358, 442
MboI GATC 2 cut(s) 356, 440
MflI RGATCY 2 cut(s) 356, 440
MhlI GDGCHC 2 cut(s) 268, 1177
MluCI AATT 4 cut(s) 482, 526, 671, 788
MlyI GAGTC 2 cut(s) 357, 379
Mph1103I ATGCAT 2 cut(s) 990, 1069
MroXI GAANNNNTTC 1 cut(s) 690
MseI TTAA 6 cut(s) 327, 375, 717, 882, 1160, 1318
MslI CAYNNNNRTG 2 cut(s) 88, 105
MspI CCGG 2 cut(s) 878, 1283
MspR9I CCNGG 4 cut(s) 261, 1170, 1194, 1284
Mva1269I GAATGC 2 cut(s) 521, 988
MvaI CCWGG 3 cut(s) 261, 1170, 1194
MvnI CGCG 1 cut(s) 319
MwoI GCNNNNNNNGC 7 cut(s) 859, 935, 944, 1016, 1186, 1189, 1255
NciI CCSGG 1 cut(s) 1284
NdeI CATATG 1 cut(s) 1212
NdeII GATC 2 cut(s) 356, 440
NlaIII CATG 2 cut(s) 135, 853
NlaIV GGNNCC 1 cut(s) 358
NmuCI GTSAC 1 cut(s) 81
NruI TCGCGA 1 cut(s) 319
NsiI ATGCAT 2 cut(s) 990, 1069
NspI RCATGY 1 cut(s) 853
OliI CACNNNNGTG 1 cut(s) 88
PctI GAATGC 2 cut(s) 521, 988
PdmI GAANNNNTTC 1 cut(s) 690
PfeI GAWTC 4 cut(s) 461, 656, 782, 796
PinAI ACCGGT 1 cut(s) 877
PkrI GCNGC 8 cut(s) 940, 1012, 1120, 1182, 1191, 1206, 1209, 1243
PleI GAGTC 2 cut(s) 356, 378
PpsI GAGTC 2 cut(s) 356, 378
PshBI ATTAAT 1 cut(s) 1160
Psp1406I AACGTT 1 cut(s) 1199
Psp6I CCWGG 3 cut(s) 259, 1168, 1192
PspGI CCWGG 3 cut(s) 259, 1168, 1192
PspN4I GGNNCC 1 cut(s) 358
PspPI GGNCC 1 cut(s) 1301
PsuI RGATCY 2 cut(s) 356, 440
RruI TCGCGA 1 cut(s) 319
RsaI GTAC 2 cut(s) 154, 185
RsaNI GTAC 2 cut(s) 153, 184
RseI CAYNNNNRTG 2 cut(s) 88, 105
SaqAI TTAA 6 cut(s) 327, 375, 717, 882, 1160, 1318
SatI GCNGC 8 cut(s) 939, 1011, 1119, 1181, 1190, 1205, 1208, 1242
Sau3AI GATC 2 cut(s) 356, 440
Sau96I GGNCC 1 cut(s) 1301
SchI GAGTC 2 cut(s) 357, 379
ScrFI CCNGG 4 cut(s) 261, 1170, 1194, 1284
SduI GDGCHC 2 cut(s) 268, 1177
SfaNI GCATC 2 cut(s) 613, 1130
SinI GGWCC 1 cut(s) 1301
SmiMI CAYNNNNRTG 2 cut(s) 88, 105
Sse9I AATT 4 cut(s) 482, 526, 671, 788
SsiI CCGC 7 cut(s) 947, 1119, 1181, 1187, 1190, 1205, 1208
StyD4I CCNGG 4 cut(s) 259, 1168, 1192, 1282
StyI CCWWGG 1 cut(s) 285
TaaI ACNGT 4 cut(s) 87, 144, 152, 1131
TaiI ACGT 2 cut(s) 595, 1202
TasI AATT 4 cut(s) 482, 526, 671, 788
TatI WGTACW 1 cut(s) 152
TauI GCSGC 5 cut(s) 1121, 1183, 1192, 1207, 1210
TfiI GAWTC 4 cut(s) 461, 656, 782, 796
Tru1I TTAA 6 cut(s) 327, 375, 717, 882, 1160, 1318
Tru9I TTAA 6 cut(s) 327, 375, 717, 882, 1160, 1318
TscAI CASTG 4 cut(s) 22, 92, 640, 1182
TseFI GTSAC 1 cut(s) 81
TseI GCWGC 3 cut(s) 938, 1010, 1241
Tsp45I GTSAC 1 cut(s) 81
TspDTI ATGAA 5 cut(s) 567, 581, 993, 1232, 1265
TspGWI ACGGA 1 cut(s) 909
TspRI CASTG 4 cut(s) 22, 92, 640, 1182
VpaK11BI GGWCC 1 cut(s) 1301
VspI ATTAAT 1 cut(s) 1160
XceI RCATGY 1 cut(s) 853
XmnI GAANNNNTTC 1 cut(s) 690
Zsp2I ATGCAT 2 cut(s) 990, 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.