pycom01g05100

Regulation of nuclear pre-mRNA domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0010009
Physical Location & Seq
Reverse (-)
4645429 .. 4650158
4730 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g05100.1

Sequence Viewer

Length: 1536 bp
ATGAATAGTGTATTCAGTGAGCAGATACTTGCAGACAAGCTCTCCAAGCTCAACAGCACCCAGCAATGCATTGAAACTTTGTCGCATTGGTGCATATTTCACCGGAGCAAAGCAGAACTGGTTGTTGCAACATGGGATAAACAATTCCATAGTGCACAGATGGTTCAGAAAGTTCCTCTCTTGTATCTTGCAAATGACATCCTGCAGAACAGTAAGCGGAAAGGAAATGAATTTGTTGCTGAGTTTTGGAAGGTTCTTCCAGCAGCTCTCAAAGTTGTTCATGAGCAAGGCGATGATCATGGAAAGAAAGTTGTATCTAGATTAGTTGGTATTTGGGAAGAGAGACGAGTATTTGGGTCTCGTACAAGGAGCCTCAAAGAACTAATGCTTGGGGAAGAACTGCCTCCACCATTGGAACTCAGTGGCAAAAAGCGCTTACGTTCAGTCAGAATTGTAAAAAGAGATTCACGCTCTATCAGAACGAAACTTTCTATTGGAGGCCCAGCTGAGAAGATTGTATCCGCATTTCACTTGGTGATCAGTGAACATCCCACTGAAGATGCTGAGATGAGTAAATGCAAGTCTGCAGCTCACCGTGTAAGGAAGTTGGAGAAAGATGTTGACATTGCCTGTACTAATGCAAAAGATCCAAAGCGGAAAACTTTAGCAAAAGAAGTAGAGGAGGAAGAAAATATTTTGAAGCAGTGTATCGATAAACTTAAATTAGTTGAAACAAGTAGAGTAGCACTTGTATCTCAATTAAAAGAAGCTCTGCATGAACAGGAATCTGAACTGGAGAACATTCGAACTCATATGCAGGTAGCACAGGCACAGGCAGAAGAAGCCACCAACATGCGGAAGCGGCTCAATGATGAAGATTATGTGTTTAAACCATCAAGTGCGACCAGTCCTTCCACTGATGGAAATTCTATAGGCGGACAAACACCTAGGAAATCAGCTGCAGCCATTGCAGCTTTGGTTGCAGACAAGCTTGCTGCTTCAAGTTCTTCTCAATTGATTATGACTTCTGTTCTTTCAACATTTGCTGCTGAAGAAGCGAAGAATGCTCGTCTGACAAAGCCCTCCACGTCTATGCCGGGCACCGATTCAATATCCAAACCTGAGAGGTCAGTGCCAGTTTCGGATGCCAATGTTTTTATGTCATCACAAGCGCCTTCTGTGCAGCCAAACCATTCATACCAATCAGCATTGGTTCCCCAACCGCAGAATCAAGCCCCAACCTCTCAAGGTCAATTTCACATGCTTCCAAACCCGCCATCTCAACAATATTTACAGCCGTCAGGAGGGGTCATGAGCCCATATGGTTATGGTAGCATTCCACATTTGCCTCTAGGGCCACCACCTCCCCCACCTCATATGGTGAGTCCACTGGTGCCTTTGGCTCAGCAGCCTGTACAAAATAGCCAGCAGCAACCAATACCCTTAACTCAGCCGCCTCCACCCGCTCCTAGTTTCCGGCCACTTCAGCAGCCACCCGGAATGGTGTATTATGCTCATCCTCATCACTCTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CTD_bind

Protein Analysis

512

Amino Acids

56.4

Weight (kDa)

9.12

Isoelectric Point (pI)

59.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CID PF04818 9 - 121 6.1e-36 CID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 808
AccB1I GGYRCC 2 cut(s) 1100, 1393
AccBSI CCGCTC 1 cut(s) 1466
AclWI GGATC 1 cut(s) 641
AcoI YGGCCR 1 cut(s) 1478
AcsI RAATTY 2 cut(s) 230, 925
AcuI CTGAAG 3 cut(s) 576, 1071, 1469
AdeI CACNNNGTG 1 cut(s) 535
AfaI GTAC 3 cut(s) 364, 634, 1416
AfeI AGCGCT 1 cut(s) 434
AfiI CCNNNNNNNGG 2 cut(s) 855, 1304
AgsI TTSAA 6 cut(s) 74, 700, 731, 1002, 1038, 1110
AjiI CACGTC 1 cut(s) 1089
AjuI GAANNNNNNNTTGG 2 cut(s) 372, 404
AluBI AGCT 9 cut(s) 40, 49, 266, 506, 590, 770, 959, 974, 991
AluI AGCT 9 cut(s) 40, 49, 266, 506, 590, 770, 959, 974, 991
Alw21I GWGCWC 1 cut(s) 157
Alw26I GTCTC 2 cut(s) 337, 363
Alw44I GTGCAC 1 cut(s) 153
AlwI GGATC 1 cut(s) 641
Aor51HI AGCGCT 1 cut(s) 434
AoxI GGCC 3 cut(s) 499, 1355, 1478
ApaLI GTGCAC 1 cut(s) 153
ApoI RAATTY 2 cut(s) 230, 925
AspA2I CCTAGG 1 cut(s) 947
AspLEI GCGC 2 cut(s) 435, 1174
AspS9I GGNCC 2 cut(s) 500, 1355
AsuC2I CCSGG 2 cut(s) 1098, 1497
AsuHPI GGTGA 4 cut(s) 92, 547, 584, 1393
AsuII TTCGAA 1 cut(s) 805
AvrII CCTAGG 1 cut(s) 947
BaeGI GKGCMC 2 cut(s) 157, 1103
BanI GGYRCC 2 cut(s) 1100, 1393
BanII GRGCYC 1 cut(s) 1319
Bbv12I GWGCWC 1 cut(s) 157
BccI CCATC 4 cut(s) 154, 901, 914, 1285
BceAI ACGGC 1 cut(s) 1282
BciVI GTATCC 1 cut(s) 529
BclI TGATCA 2 cut(s) 295, 537
BcnI CCSGG 2 cut(s) 1098, 1497
BcoDI GTCTC 2 cut(s) 337, 363
BfaI CTAG 4 cut(s) 318, 948, 1352, 1470
BfmI CTRYAG 4 cut(s) 203, 585, 930, 960
BfoI RGCGCY 2 cut(s) 436, 1175
BfuAI ACCTGC 1 cut(s) 808
BfuI GTATCC 1 cut(s) 529
BglI GCCNNNNNGGC 1 cut(s) 1354
BlnI CCTAGG 1 cut(s) 947
BlpI GCTNAGC 1 cut(s) 1404
Bme1390I CCNGG 2 cut(s) 1098, 1497
BmgBI CACGTC 1 cut(s) 1089
BmgT120I GGNCC 2 cut(s) 500, 1355
BmiI GGNNCC 4 cut(s) 371, 1102, 1215, 1395
BmrFI CCNGG 2 cut(s) 1098, 1497
BmsI GCATC 2 cut(s) 550, 1135
BpmI CTGGAG 1 cut(s) 815
Bpu1102I GCTNAGC 1 cut(s) 1404
Bpu14I TTCGAA 1 cut(s) 805
BpuEI CTTGAG 1 cut(s) 1230
BpuMI CCSGG 2 cut(s) 1098, 1497
Bsa29I ATCGAT 1 cut(s) 711
BsaI GGTCTC 1 cut(s) 363
BsaJI CCNNGG 1 cut(s) 947
BsaWI WCCGGW 1 cut(s) 102
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 2 cut(s) 855, 1304
Bse1I ACTGG 5 cut(s) 123, 798, 906, 1136, 1395
Bse3DI GCAATG 3 cut(s) 71, 624, 966
BseCI ATCGAT 1 cut(s) 711
BseDI CCNNGG 1 cut(s) 947
BseGI GGATG 4 cut(s) 198, 547, 1150, 1516
BseLI CCNNNNNNNGG 2 cut(s) 855, 1304
BseMI GCAATG 3 cut(s) 71, 624, 966
BseMII CTCAG 7 cut(s) 231, 433, 498, 555, 1113, 1418, 1463
BseNI ACTGG 5 cut(s) 123, 798, 906, 1136, 1395
BseRI GAGGAG 1 cut(s) 695
BseSI GKGCMC 2 cut(s) 157, 1103
BseYI CCCAGC 2 cut(s) 60, 502
BsgI GTGCAG 1 cut(s) 1202
BshFI GGCC 3 cut(s) 501, 1357, 1480
BshNI GGYRCC 2 cut(s) 1100, 1393
BshVI ATCGAT 1 cut(s) 711
BsiHKAI GWGCWC 1 cut(s) 157
BsiSI CCGG 4 cut(s) 103, 1097, 1477, 1497
BslI CCNNNNNNNGG 2 cut(s) 855, 1304
BsmAI GTCTC 2 cut(s) 337, 363
BsmBI CGTCTC 1 cut(s) 337
BsmI GAATGC 2 cut(s) 1069, 1335
BsnI GGCC 3 cut(s) 501, 1357, 1480
Bso31I GGTCTC 1 cut(s) 363
Bsp119I TTCGAA 1 cut(s) 805
Bsp1286I GDGCHC 3 cut(s) 157, 1103, 1319
Bsp1407I TGTACA 1 cut(s) 1414
Bsp143I GATC 3 cut(s) 295, 537, 646
Bsp1720I GCTNAGC 1 cut(s) 1404
BspANI GGCC 3 cut(s) 501, 1357, 1480
BspCNI CTCAG 7 cut(s) 232, 432, 499, 556, 1114, 1417, 1462
BspDI ATCGAT 1 cut(s) 711
BspHI TCATGA 2 cut(s) 280, 1311
BspLI GGNNCC 4 cut(s) 371, 1102, 1215, 1395
BspMAI CTGCAG 3 cut(s) 207, 589, 964
BspMI ACCTGC 1 cut(s) 808
BspPI GGATC 1 cut(s) 641
BspT104I TTCGAA 1 cut(s) 805
BspT107I GGYRCC 2 cut(s) 1100, 1393
BspTNI GGTCTC 1 cut(s) 363
BsrBI CCGCTC 1 cut(s) 1466
BsrDI GCAATG 3 cut(s) 71, 624, 966
BsrGI TGTACA 1 cut(s) 1414
BsrI ACTGG 5 cut(s) 123, 798, 906, 1136, 1395
BssECI CCNNGG 1 cut(s) 947
BssMI GATC 3 cut(s) 295, 537, 646
BssT1I CCWWGG 1 cut(s) 947
Bst4CI ACNGT 2 cut(s) 212, 596
Bst6I CTCTTC 1 cut(s) 333
BstAPI GCANNNNNTGC 1 cut(s) 968
BstAUI TGTACA 1 cut(s) 1414
BstBI TTCGAA 1 cut(s) 805
BstC8I GCNNGC 2 cut(s) 993, 1427
BstDEI CTNAG 8 cut(s) 240, 419, 507, 564, 1122, 1404, 1449, 1530
BstF5I GGATG 4 cut(s) 198, 547, 1150, 1516
BstH2I RGCGCY 2 cut(s) 436, 1175
BstHHI GCGC 2 cut(s) 435, 1174
BstKTI GATC 3 cut(s) 298, 540, 649
BstMAI GTCTC 2 cut(s) 337, 363
BstMBI GATC 3 cut(s) 295, 537, 646
BstNSI RCATGY 2 cut(s) 856, 1264
BstSCI CCNGG 2 cut(s) 1096, 1495
BstSFI CTRYAG 4 cut(s) 203, 585, 930, 960
BstSLI GKGCMC 2 cut(s) 157, 1103
BstX2I RGATCY 1 cut(s) 646
BstYI RGATCY 1 cut(s) 646
Bsu15I ATCGAT 1 cut(s) 711
BsuI GTATCC 1 cut(s) 529
BsuRI GGCC 3 cut(s) 501, 1357, 1480
BsuTUI ATCGAT 1 cut(s) 711
BtgZI GCGATG 1 cut(s) 306
BtrI CACGTC 1 cut(s) 1089
BtsCI GGATG 4 cut(s) 198, 547, 1150, 1516
BtsI GCAGTG 1 cut(s) 710
BtsIMutI CAGTG 8 cut(s) 22, 427, 547, 552, 710, 915, 1137, 1388
BveI ACCTGC 1 cut(s) 808
Cac8I GCNNGC 2 cut(s) 993, 1427
CciI TCATGA 2 cut(s) 280, 1311
CfoI GCGC 2 cut(s) 435, 1174
Cfr13I GGNCC 2 cut(s) 500, 1355
ClaI ATCGAT 1 cut(s) 711
Csp6I GTAC 3 cut(s) 363, 633, 1415
CviAII CATG 7 cut(s) 132, 281, 299, 776, 853, 1261, 1312
CviQI GTAC 3 cut(s) 363, 633, 1415
DdeI CTNAG 8 cut(s) 240, 419, 507, 564, 1122, 1404, 1449, 1530
DpnI GATC 3 cut(s) 297, 539, 648
DpnII GATC 3 cut(s) 295, 537, 646
DraI TTTAAA 1 cut(s) 889
DraIII CACNNNGTG 1 cut(s) 535
EaeI YGGCCR 1 cut(s) 1478
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
EciI GGCGGA 1 cut(s) 951
Eco130I CCWWGG 1 cut(s) 947
Eco24I GRGCYC 1 cut(s) 1319
Eco31I GGTCTC 1 cut(s) 363
Eco47III AGCGCT 1 cut(s) 434
Eco57I CTGAAG 3 cut(s) 576, 1071, 1469
EcoT14I CCWWGG 1 cut(s) 947
EcoT22I ATGCAT 1 cut(s) 71
EcoT38I GRGCYC 1 cut(s) 1319
ErhI CCWWGG 1 cut(s) 947
Esp3I CGTCTC 1 cut(s) 337
FaeI CATG 7 cut(s) 135, 284, 302, 779, 856, 1264, 1315
FatI CATG 7 cut(s) 131, 280, 298, 775, 852, 1260, 1311
FauI CCCGC 2 cut(s) 1281, 1471
FauNDI CATATG 3 cut(s) 813, 1321, 1377
FbaI TGATCA 2 cut(s) 295, 537
FokI GGATG 4 cut(s) 185, 534, 1157, 1503
FriOI GRGCYC 1 cut(s) 1319
FspBI CTAG 4 cut(s) 318, 948, 1352, 1470
GlaI GCGC 2 cut(s) 434, 1173
GsaI CCCAGC 2 cut(s) 64, 506
GsuI CTGGAG 1 cut(s) 815
HaeII RGCGCY 2 cut(s) 436, 1175
HaeIII GGCC 3 cut(s) 501, 1357, 1480
HapII CCGG 4 cut(s) 103, 1097, 1477, 1497
HhaI GCGC 2 cut(s) 435, 1174
Hin1II CATG 7 cut(s) 135, 284, 302, 779, 856, 1264, 1315
Hin6I GCGC 2 cut(s) 433, 1172
HinP1I GCGC 2 cut(s) 433, 1172
HincII GTYRAC 1 cut(s) 622
HindII GTYRAC 1 cut(s) 622
HindIII AAGCTT 1 cut(s) 989
HinfI GANTC 5 cut(s) 464, 785, 1106, 1228, 1384
HpaII CCGG 4 cut(s) 103, 1097, 1477, 1497
HphI GGTGA 4 cut(s) 92, 547, 584, 1393
Hpy166II GTNNAC 4 cut(s) 155, 545, 622, 1388
Hpy188I TCNGA 6 cut(s) 168, 449, 479, 790, 1074, 1144
Hpy188III TCNNGA 4 cut(s) 281, 318, 1302, 1312
Hpy8I GTNNAC 4 cut(s) 155, 545, 622, 1388
HpyAV CCTTC 3 cut(s) 244, 921, 1185
HpyCH4III ACNGT 2 cut(s) 212, 596
HpyCH4IV ACGT 2 cut(s) 439, 1088
HpyF3I CTNAG 8 cut(s) 240, 419, 507, 564, 1122, 1404, 1449, 1530
HpySE526I ACGT 2 cut(s) 439, 1088
Hsp92II CATG 7 cut(s) 135, 284, 302, 779, 856, 1264, 1315
HspAI GCGC 2 cut(s) 433, 1172
Ksp22I TGATCA 2 cut(s) 295, 537
Kzo9I GATC 3 cut(s) 295, 537, 646
LmnI GCTCC 3 cut(s) 105, 369, 1471
LweI GCATC 2 cut(s) 550, 1135
MaeI CTAG 4 cut(s) 318, 948, 1352, 1470
MaeII ACGT 2 cut(s) 439, 1088
MalI GATC 3 cut(s) 297, 539, 648
MbiI CCGCTC 1 cut(s) 1466
MboI GATC 3 cut(s) 295, 537, 646
MfeI CAATTG 1 cut(s) 1013
MflI RGATCY 1 cut(s) 646
MhlI GDGCHC 3 cut(s) 157, 1103, 1319
MluCI AATT 8 cut(s) 143, 230, 450, 722, 758, 925, 1013, 1253
MlyI GAGTC 1 cut(s) 1393
MmeI TCCRAC 1 cut(s) 588
Mph1103I ATGCAT 1 cut(s) 71
MseI TTAA 4 cut(s) 720, 761, 888, 1445
MslI CAYNNNNRTG 2 cut(s) 851, 1091
MspA1I CMGCKG 2 cut(s) 506, 959
MspI CCGG 4 cut(s) 103, 1097, 1477, 1497
MspR9I CCNGG 2 cut(s) 1098, 1497
MssI GTTTAAAC 1 cut(s) 889
MunI CAATTG 1 cut(s) 1013
Mva1269I GAATGC 2 cut(s) 1069, 1335
NciI CCSGG 2 cut(s) 1098, 1497
NdeI CATATG 3 cut(s) 813, 1321, 1377
NdeII GATC 3 cut(s) 295, 537, 646
NlaIII CATG 7 cut(s) 135, 284, 302, 779, 856, 1264, 1315
NlaIV GGNNCC 4 cut(s) 371, 1102, 1215, 1395
NsiI ATGCAT 1 cut(s) 71
NspI RCATGY 2 cut(s) 856, 1264
NspV TTCGAA 1 cut(s) 805
PagI TCATGA 2 cut(s) 280, 1311
PctI GAATGC 2 cut(s) 1069, 1335
PfeI GAWTC 4 cut(s) 464, 785, 1106, 1228
PleI GAGTC 1 cut(s) 1392
PmeI GTTTAAAC 1 cut(s) 889
PpsI GAGTC 1 cut(s) 1392
PspFI CCCAGC 2 cut(s) 60, 502
PspN4I GGNNCC 4 cut(s) 371, 1102, 1215, 1395
PspPI GGNCC 2 cut(s) 500, 1355
PstI CTGCAG 3 cut(s) 207, 589, 964
PsuI RGATCY 1 cut(s) 646
PvuII CAGCTG 2 cut(s) 506, 959
RsaI GTAC 3 cut(s) 364, 634, 1416
RsaNI GTAC 3 cut(s) 363, 633, 1415
RseI CAYNNNNRTG 2 cut(s) 851, 1091
SaqAI TTAA 4 cut(s) 720, 761, 888, 1445
Sau3AI GATC 3 cut(s) 295, 537, 646
Sau96I GGNCC 2 cut(s) 500, 1355
SchI GAGTC 1 cut(s) 1393
ScrFI CCNGG 2 cut(s) 1098, 1497
SduI GDGCHC 3 cut(s) 157, 1103, 1319
SfaNI GCATC 2 cut(s) 550, 1135
SfcI CTRYAG 4 cut(s) 203, 585, 930, 960
SfuI TTCGAA 1 cut(s) 805
SmiMI CAYNNNNRTG 2 cut(s) 851, 1091
SmlI CTYRAG 1 cut(s) 1245
SmoI CTYRAG 1 cut(s) 1245
Sse9I AATT 8 cut(s) 143, 230, 450, 722, 758, 925, 1013, 1253
SspI AATATT 2 cut(s) 694, 1289
SspMI CTAG 4 cut(s) 318, 948, 1352, 1470
StyD4I CCNGG 2 cut(s) 1096, 1495
StyI CCWWGG 1 cut(s) 947
TaaI ACNGT 2 cut(s) 212, 596
TaiI ACGT 2 cut(s) 442, 1091
TaqI TCGA 2 cut(s) 711, 805
TasI AATT 8 cut(s) 143, 230, 450, 722, 758, 925, 1013, 1253
TatI WGTACW 2 cut(s) 632, 1414
TauI GCSGC 2 cut(s) 865, 1456
TfiI GAWTC 4 cut(s) 464, 785, 1106, 1228
Tru1I TTAA 4 cut(s) 720, 761, 888, 1445
Tru9I TTAA 4 cut(s) 720, 761, 888, 1445
TscAI CASTG 8 cut(s) 22, 427, 547, 559, 710, 922, 1137, 1395
TspDTI ATGAA 6 cut(s) 17, 243, 269, 792, 888, 1185
TspRI CASTG 8 cut(s) 22, 427, 547, 559, 710, 922, 1137, 1395
VneI GTGCAC 1 cut(s) 153
XapI RAATTY 2 cut(s) 230, 925
XbaI TCTAGA 1 cut(s) 317
XceI RCATGY 2 cut(s) 856, 1264
XcmI CCANNNNNNNNNTGG 1 cut(s) 973
XmaJI CCTAGG 1 cut(s) 947
XspI CTAG 4 cut(s) 318, 948, 1352, 1470
Zsp2I ATGCAT 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.