MD01G1022500.v1.1

Regulation of nuclear pre-mRNA domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0010009
Physical Location & Seq
Reverse (-)
8959054 .. 8964512
5459 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1022500.v1.1.491

Sequence Viewer

Length: 1557 bp
ATGAATAGTGTATTCAGTGAGCAGATACTTGCAGACAAGCTCTCCAAGCTCAACAGCACCCAGCAATGCATTGAAACTTTGTCGCATTGGTGCATATTTCACCGGAGCAAAGCAGAACTGGTTGTTGAAACATGGGATAAACAATTCCATAGTGCACAGATGGTTCAGAAAGTTCCTCTCTTGTATCTTGCAAATGACATCCTGCAGAACAGTAAGCGGAAAGGAAACGAATTTGTTGCTGAGTTTTGGAAGGTTCTTCCAGCCGCTCTCAAAGTTGTTCACGAGCAAGGCGATGATCATGGAAAGAAAGTTGTATCTAGATTAGTTGGTATTTGGGAAGAGAGACGAGTATTTGGGTCTCGTACCAGGAGCCTCAAAGAACTAATGCTTGGGGAAGAACTGCCTCCACCATTGGAACTCAGTGGCAAAAAGCGCTCACGTTCAGTCAGAATTGTAAAAAGAGACTCACGCTCTATCAGAACGAAACTTTCTATTGGAGGCCCGGCTGAGAAGATTGTATCCGCATTTCACTTGGTGATCAGTGAACATCCCACTGAAGATACTGAGATGAGTAAATGCAAGTCTGCAGCTCACCGTGTAAGGAAGTTGGAGAAAGATGTTGACATTGCCTGTACTAATGCAAAAGATCCAAAGTGGAAAACTTTAGCAAAAGAAGTAGAGGAGGAAGAAAATATTTTGAAGCAGTGTATCGATAAACTTAAATTAGTTGAAACAAGTAGAGTAGCACTTGTATCTCAATTACAAGAAGCTCTGCATGAACAGGAATCTGAACTGGAGAACGTTCGAACTCATATGCAGGTGGCACAGGCACAGGCAGAAGAAGCCACCAACATGCGGAAGCGGCTCAATGATGAAGATTATGTGTTTAAACCATCAAGTGCGACCAGTCCTTCAACTGATGGAAATTCTATAGGCGGACAAACACCTAGGAAATCAGCTGCAGCCATTGCAGCTTTGGTTGCAGACAAGCTTGCTGCATCAAGTTCTTCTCAATTGATTATGACTTCTGTTCTTTCAACATTTGCTGCTGAAGAAGCGAAGAATGCTGGTCTGACAAAGCCCTCCACGTCTATGCCGGGCACCAATTCAATATCCAAACCTGAGAGGTCAGTGCCAGTTTCAGATGCCAATGTTTTTATGTCATCACAAGCGCCTTCTGTGCAGCCAAACCATTCATACCAATCAGCGTTGGTTCCCCAACTGCAGAATCAAGCCCCAACATCTCAAGGTCAATTTCACATGCTTCCAAACCCGCCATCTCAACAATATTTACAGCCGTCAGGAGGGGTCATGAGCCCGTATGGTTATGGTAGCATTCCACATTTGCCTCTAGGGCCACCACCTCCCCCACCTCATATGGTGAGTCCAATGGTGCCTTTGGCTCAGCAGCCACTGCAAATGAATCAGCAGCCTGTACAAAATAGCCAGCAGCAACTGATACCCTTAACTCAGCCGCCTCCACCCGCTCCTAGTTTCCGGCCACTTCAGCAGCCACCCGGAATGGTGTATTATGCTCATCCTCATCACTCTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CTD_bind

Protein Analysis

519

Amino Acids

57.27

Weight (kDa)

8.86

Isoelectric Point (pI)

59.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CID PF04818 9 - 121 3.2e-36 CID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 808
Acc36I ACCTGC 1 cut(s) 808
AccB1I GGYRCC 2 cut(s) 1100, 1393
AccBSI CCGCTC 2 cut(s) 266, 1487
AciI CCGC 9 cut(s) 217, 264, 522, 856, 862, 936, 1274, 1475, 1485
AclI AACGTT 1 cut(s) 801
AclWI GGATC 1 cut(s) 641
AcoI YGGCCR 1 cut(s) 1499
AcsI RAATTY 2 cut(s) 230, 925
AcuI CTGAAG 3 cut(s) 576, 1071, 1490
AdeI CACNNNGTG 1 cut(s) 535
AfaI GTAC 3 cut(s) 364, 634, 1437
AfeI AGCGCT 1 cut(s) 434
AfiI CCNNNNNNNGG 2 cut(s) 855, 1304
AgsI TTSAA 7 cut(s) 74, 128, 700, 731, 915, 1038, 1110
AjiI CACGTC 1 cut(s) 1089
AjnI CCWGG 1 cut(s) 365
AjuI GAANNNNNNNTTGG 2 cut(s) 372, 404
AluBI AGCT 7 cut(s) 40, 49, 590, 770, 959, 974, 991
AluI AGCT 7 cut(s) 40, 49, 590, 770, 959, 974, 991
Alw21I GWGCWC 1 cut(s) 157
Alw26I GTCTC 3 cut(s) 337, 363, 456
Alw44I GTGCAC 1 cut(s) 153
AlwI GGATC 1 cut(s) 641
AlwNI CAGNNNCTG 2 cut(s) 1414, 1456
Aor51HI AGCGCT 1 cut(s) 434
AoxI GGCC 3 cut(s) 499, 1355, 1499
ApaLI GTGCAC 1 cut(s) 153
ApoI RAATTY 2 cut(s) 230, 925
AspA2I CCTAGG 1 cut(s) 947
AspLEI GCGC 2 cut(s) 435, 1174
AspS9I GGNCC 2 cut(s) 500, 1355
AsuC2I CCSGG 3 cut(s) 503, 1098, 1518
AsuHPI GGTGA 4 cut(s) 92, 547, 584, 1393
AsuII TTCGAA 1 cut(s) 805
AvrII CCTAGG 1 cut(s) 947
BaeGI GKGCMC 2 cut(s) 157, 1103
BanI GGYRCC 2 cut(s) 1100, 1393
BanII GRGCYC 1 cut(s) 1319
BauI CACGAG 1 cut(s) 281
Bbv12I GWGCWC 1 cut(s) 157
BccI CCATC 4 cut(s) 154, 901, 914, 1285
BceAI ACGGC 1 cut(s) 1282
BcgI CGANNNNNNTGC 2 cut(s) 218, 252
BciT130I CCWGG 1 cut(s) 367
BciVI GTATCC 1 cut(s) 529
BclI TGATCA 2 cut(s) 295, 537
BcnI CCSGG 3 cut(s) 503, 1098, 1518
BcoDI GTCTC 3 cut(s) 337, 363, 456
BfaI CTAG 4 cut(s) 318, 948, 1352, 1491
BfmI CTRYAG 5 cut(s) 203, 585, 930, 960, 1223
BfoI RGCGCY 2 cut(s) 436, 1175
BfuAI ACCTGC 1 cut(s) 808
BfuI GTATCC 1 cut(s) 529
BglI GCCNNNNNGGC 1 cut(s) 1354
BlnI CCTAGG 1 cut(s) 947
BlpI GCTNAGC 1 cut(s) 1404
Bme1390I CCNGG 4 cut(s) 367, 503, 1098, 1518
BmgBI CACGTC 1 cut(s) 1089
BmgT120I GGNCC 2 cut(s) 500, 1355
BmiI GGNNCC 4 cut(s) 371, 1102, 1215, 1395
BmrFI CCNGG 4 cut(s) 367, 503, 1098, 1518
BmsI GCATC 2 cut(s) 1007, 1135
BpmI CTGGAG 1 cut(s) 815
Bpu1102I GCTNAGC 1 cut(s) 1404
Bpu14I TTCGAA 1 cut(s) 805
BpuEI CTTGAG 1 cut(s) 1230
BpuMI CCSGG 3 cut(s) 503, 1098, 1518
Bsa29I ATCGAT 1 cut(s) 711
BsaI GGTCTC 1 cut(s) 363
BsaJI CCNNGG 1 cut(s) 947
BsaWI WCCGGW 1 cut(s) 102
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 2 cut(s) 855, 1304
Bse1I ACTGG 4 cut(s) 123, 798, 906, 1136
Bse3DI GCAATG 3 cut(s) 71, 624, 966
BseBI CCWGG 1 cut(s) 367
BseCI ATCGAT 1 cut(s) 711
BseDI CCNNGG 1 cut(s) 947
BseGI GGATG 3 cut(s) 198, 547, 1537
BseLI CCNNNNNNNGG 2 cut(s) 855, 1304
BseMI GCAATG 3 cut(s) 71, 624, 966
BseMII CTCAG 7 cut(s) 231, 433, 498, 555, 1113, 1418, 1484
BseNI ACTGG 4 cut(s) 123, 798, 906, 1136
BseRI GAGGAG 1 cut(s) 695
BseSI GKGCMC 2 cut(s) 157, 1103
BseYI CCCAGC 1 cut(s) 60
BsgI GTGCAG 1 cut(s) 1202
BshFI GGCC 3 cut(s) 501, 1357, 1501
BshNI GGYRCC 2 cut(s) 1100, 1393
BshVI ATCGAT 1 cut(s) 711
BsiHKAI GWGCWC 1 cut(s) 157
BsiSI CCGG 5 cut(s) 103, 503, 1097, 1498, 1518
BslI CCNNNNNNNGG 2 cut(s) 855, 1304
BsmAI GTCTC 3 cut(s) 337, 363, 456
BsmBI CGTCTC 1 cut(s) 337
BsmI GAATGC 2 cut(s) 1069, 1335
BsnI GGCC 3 cut(s) 501, 1357, 1501
Bso31I GGTCTC 1 cut(s) 363
Bsp119I TTCGAA 1 cut(s) 805
Bsp1286I GDGCHC 3 cut(s) 157, 1103, 1319
Bsp1407I TGTACA 1 cut(s) 1435
Bsp143I GATC 3 cut(s) 295, 537, 646
Bsp1720I GCTNAGC 1 cut(s) 1404
BspACI CCGC 9 cut(s) 217, 264, 522, 856, 862, 936, 1274, 1475, 1485
BspANI GGCC 3 cut(s) 501, 1357, 1501
BspCNI CTCAG 7 cut(s) 232, 432, 499, 556, 1114, 1417, 1483
BspDI ATCGAT 1 cut(s) 711
BspHI TCATGA 1 cut(s) 1311
BspLI GGNNCC 4 cut(s) 371, 1102, 1215, 1395
BspMAI CTGCAG 4 cut(s) 207, 589, 964, 1227
BspMI ACCTGC 1 cut(s) 808
BspPI GGATC 1 cut(s) 641
BspT104I TTCGAA 1 cut(s) 805
BspT107I GGYRCC 2 cut(s) 1100, 1393
BspTNI GGTCTC 1 cut(s) 363
BsrBI CCGCTC 2 cut(s) 266, 1487
BsrDI GCAATG 3 cut(s) 71, 624, 966
BsrGI TGTACA 1 cut(s) 1435
BsrI ACTGG 4 cut(s) 123, 798, 906, 1136
BssECI CCNNGG 1 cut(s) 947
BssMI GATC 3 cut(s) 295, 537, 646
BssSI CACGAG 1 cut(s) 281
BssT1I CCWWGG 1 cut(s) 947
Bst2BI CACGAG 1 cut(s) 281
Bst2UI CCWGG 1 cut(s) 367
Bst4CI ACNGT 2 cut(s) 212, 596
Bst6I CTCTTC 1 cut(s) 333
BstAPI GCANNNNNTGC 2 cut(s) 968, 1414
BstAUI TGTACA 1 cut(s) 1435
BstBI TTCGAA 1 cut(s) 805
BstC8I GCNNGC 2 cut(s) 993, 1448
BstDEI CTNAG 8 cut(s) 240, 419, 507, 564, 1122, 1404, 1470, 1551
BstF5I GGATG 3 cut(s) 198, 547, 1537
BstH2I RGCGCY 2 cut(s) 436, 1175
BstHHI GCGC 2 cut(s) 435, 1174
BstKTI GATC 3 cut(s) 298, 540, 649
BstMAI GTCTC 3 cut(s) 337, 363, 456
BstMBI GATC 3 cut(s) 295, 537, 646
BstNI CCWGG 1 cut(s) 367
BstNSI RCATGY 2 cut(s) 856, 1264
BstSCI CCNGG 4 cut(s) 365, 501, 1096, 1516
BstSFI CTRYAG 5 cut(s) 203, 585, 930, 960, 1223
BstSLI GKGCMC 2 cut(s) 157, 1103
BstX2I RGATCY 1 cut(s) 646
BstYI RGATCY 1 cut(s) 646
Bsu15I ATCGAT 1 cut(s) 711
BsuI GTATCC 1 cut(s) 529
BsuRI GGCC 3 cut(s) 501, 1357, 1501
BsuTUI ATCGAT 1 cut(s) 711
BtgZI GCGATG 1 cut(s) 306
BtrI CACGTC 1 cut(s) 1089
BtsCI GGATG 3 cut(s) 198, 547, 1537
BtsI GCAGTG 2 cut(s) 710, 1412
BtsIMutI CAGTG 7 cut(s) 22, 427, 547, 552, 710, 1137, 1412
BveI ACCTGC 1 cut(s) 808
Cac8I GCNNGC 2 cut(s) 993, 1448
CaiI CAGNNNCTG 2 cut(s) 1414, 1456
CciI TCATGA 1 cut(s) 1311
CfoI GCGC 2 cut(s) 435, 1174
Cfr13I GGNCC 2 cut(s) 500, 1355
ClaI ATCGAT 1 cut(s) 711
Csp6I GTAC 3 cut(s) 363, 633, 1436
CviAII CATG 6 cut(s) 132, 299, 776, 853, 1261, 1312
CviQI GTAC 3 cut(s) 363, 633, 1436
DdeI CTNAG 8 cut(s) 240, 419, 507, 564, 1122, 1404, 1470, 1551
DpnI GATC 3 cut(s) 297, 539, 648
DpnII GATC 3 cut(s) 295, 537, 646
DraI TTTAAA 1 cut(s) 889
DraIII CACNNNGTG 1 cut(s) 535
EaeI YGGCCR 1 cut(s) 1499
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
EciI GGCGGA 1 cut(s) 951
Eco130I CCWWGG 1 cut(s) 947
Eco24I GRGCYC 1 cut(s) 1319
Eco31I GGTCTC 1 cut(s) 363
Eco47III AGCGCT 1 cut(s) 434
Eco57I CTGAAG 3 cut(s) 576, 1071, 1490
EcoRII CCWGG 1 cut(s) 365
EcoT14I CCWWGG 1 cut(s) 947
EcoT22I ATGCAT 1 cut(s) 71
EcoT38I GRGCYC 1 cut(s) 1319
ErhI CCWWGG 1 cut(s) 947
Esp3I CGTCTC 1 cut(s) 337
FaeI CATG 6 cut(s) 135, 302, 779, 856, 1264, 1315
FatI CATG 6 cut(s) 131, 298, 775, 852, 1260, 1311
FauI CCCGC 2 cut(s) 1281, 1492
FauNDI CATATG 2 cut(s) 813, 1377
FbaI TGATCA 2 cut(s) 295, 537
FokI GGATG 3 cut(s) 185, 534, 1524
FriOI GRGCYC 1 cut(s) 1319
FspBI CTAG 4 cut(s) 318, 948, 1352, 1491
GlaI GCGC 2 cut(s) 434, 1173
GsaI CCCAGC 1 cut(s) 64
GsuI CTGGAG 1 cut(s) 815
HaeII RGCGCY 2 cut(s) 436, 1175
HaeIII GGCC 3 cut(s) 501, 1357, 1501
HapII CCGG 5 cut(s) 103, 503, 1097, 1498, 1518
HhaI GCGC 2 cut(s) 435, 1174
Hin1II CATG 6 cut(s) 135, 302, 779, 856, 1264, 1315
Hin6I GCGC 2 cut(s) 433, 1172
HinP1I GCGC 2 cut(s) 433, 1172
HincII GTYRAC 1 cut(s) 622
HindII GTYRAC 1 cut(s) 622
HindIII AAGCTT 1 cut(s) 989
HinfI GANTC 5 cut(s) 464, 785, 1228, 1384, 1423
HpaII CCGG 5 cut(s) 103, 503, 1097, 1498, 1518
HphI GGTGA 4 cut(s) 92, 547, 584, 1393
Hpy166II GTNNAC 4 cut(s) 155, 280, 545, 622
Hpy188I TCNGA 6 cut(s) 168, 449, 479, 790, 1074, 1144
Hpy188III TCNNGA 4 cut(s) 281, 318, 1302, 1312
Hpy8I GTNNAC 4 cut(s) 155, 280, 545, 622
HpyAV CCTTC 3 cut(s) 244, 921, 1185
HpyCH4III ACNGT 2 cut(s) 212, 596
HpyCH4IV ACGT 3 cut(s) 439, 801, 1088
HpyF3I CTNAG 8 cut(s) 240, 419, 507, 564, 1122, 1404, 1470, 1551
HpySE526I ACGT 3 cut(s) 439, 801, 1088
Hsp92II CATG 6 cut(s) 135, 302, 779, 856, 1264, 1315
HspAI GCGC 2 cut(s) 433, 1172
Ksp22I TGATCA 2 cut(s) 295, 537
Kzo9I GATC 3 cut(s) 295, 537, 646
LmnI GCTCC 3 cut(s) 105, 369, 1492
LweI GCATC 2 cut(s) 1007, 1135
MaeI CTAG 4 cut(s) 318, 948, 1352, 1491
MaeII ACGT 3 cut(s) 439, 801, 1088
MalI GATC 3 cut(s) 297, 539, 648
MbiI CCGCTC 2 cut(s) 266, 1487
MboI GATC 3 cut(s) 295, 537, 646
MfeI CAATTG 1 cut(s) 1013
MflI RGATCY 1 cut(s) 646
MhlI GDGCHC 3 cut(s) 157, 1103, 1319
MluCI AATT 9 cut(s) 143, 230, 450, 722, 758, 925, 1013, 1105, 1253
MlyI GAGTC 2 cut(s) 458, 1393
MmeI TCCRAC 1 cut(s) 588
Mph1103I ATGCAT 1 cut(s) 71
MseI TTAA 3 cut(s) 720, 888, 1466
MslI CAYNNNNRTG 2 cut(s) 851, 1091
MspA1I CMGCKG 1 cut(s) 959
MspI CCGG 5 cut(s) 103, 503, 1097, 1498, 1518
MspR9I CCNGG 4 cut(s) 367, 503, 1098, 1518
MssI GTTTAAAC 1 cut(s) 889
MunI CAATTG 1 cut(s) 1013
Mva1269I GAATGC 2 cut(s) 1069, 1335
MvaI CCWGG 1 cut(s) 367
NciI CCSGG 3 cut(s) 503, 1098, 1518
NdeI CATATG 2 cut(s) 813, 1377
NdeII GATC 3 cut(s) 295, 537, 646
NlaIII CATG 6 cut(s) 135, 302, 779, 856, 1264, 1315
NlaIV GGNNCC 4 cut(s) 371, 1102, 1215, 1395
NsiI ATGCAT 1 cut(s) 71
NspI RCATGY 2 cut(s) 856, 1264
NspV TTCGAA 1 cut(s) 805
PagI TCATGA 1 cut(s) 1311
PaqCI CACCTGC 1 cut(s) 808
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PctI GAATGC 2 cut(s) 1069, 1335
PfeI GAWTC 3 cut(s) 785, 1228, 1423
PleI GAGTC 2 cut(s) 458, 1392
PmeI GTTTAAAC 1 cut(s) 889
PpsI GAGTC 2 cut(s) 458, 1392
Psp1406I AACGTT 1 cut(s) 801
Psp6I CCWGG 1 cut(s) 365
PspFI CCCAGC 1 cut(s) 60
PspGI CCWGG 1 cut(s) 365
PspN4I GGNNCC 4 cut(s) 371, 1102, 1215, 1395
PspPI GGNCC 2 cut(s) 500, 1355
PstI CTGCAG 4 cut(s) 207, 589, 964, 1227
PstNI CAGNNNCTG 2 cut(s) 1414, 1456
PsuI RGATCY 1 cut(s) 646
PvuII CAGCTG 1 cut(s) 959
RsaI GTAC 3 cut(s) 364, 634, 1437
RsaNI GTAC 3 cut(s) 363, 633, 1436
RseI CAYNNNNRTG 2 cut(s) 851, 1091
SaqAI TTAA 3 cut(s) 720, 888, 1466
Sau3AI GATC 3 cut(s) 295, 537, 646
Sau96I GGNCC 2 cut(s) 500, 1355
SchI GAGTC 2 cut(s) 458, 1393
ScrFI CCNGG 4 cut(s) 367, 503, 1098, 1518
SduI GDGCHC 3 cut(s) 157, 1103, 1319
SfaNI GCATC 2 cut(s) 1007, 1135
SfcI CTRYAG 5 cut(s) 203, 585, 930, 960, 1223
SfuI TTCGAA 1 cut(s) 805
SmiMI CAYNNNNRTG 2 cut(s) 851, 1091
SmlI CTYRAG 1 cut(s) 1245
SmoI CTYRAG 1 cut(s) 1245
Sse9I AATT 9 cut(s) 143, 230, 450, 722, 758, 925, 1013, 1105, 1253
SsiI CCGC 9 cut(s) 217, 264, 522, 856, 862, 936, 1274, 1475, 1485
SspI AATATT 2 cut(s) 694, 1289
SspMI CTAG 4 cut(s) 318, 948, 1352, 1491
StyD4I CCNGG 4 cut(s) 365, 501, 1096, 1516
StyI CCWWGG 1 cut(s) 947
TaaI ACNGT 2 cut(s) 212, 596
TaiI ACGT 3 cut(s) 442, 804, 1091
TaqI TCGA 2 cut(s) 711, 805
TasI AATT 9 cut(s) 143, 230, 450, 722, 758, 925, 1013, 1105, 1253
TatI WGTACW 2 cut(s) 632, 1435
TauI GCSGC 3 cut(s) 266, 865, 1477
TfiI GAWTC 3 cut(s) 785, 1228, 1423
Tru1I TTAA 3 cut(s) 720, 888, 1466
Tru9I TTAA 3 cut(s) 720, 888, 1466
TscAI CASTG 7 cut(s) 22, 427, 547, 559, 710, 1137, 1419
TspDTI ATGAA 5 cut(s) 17, 792, 888, 1185, 1436
TspRI CASTG 7 cut(s) 22, 427, 547, 559, 710, 1137, 1419
VneI GTGCAC 1 cut(s) 153
XapI RAATTY 2 cut(s) 230, 925
XbaI TCTAGA 1 cut(s) 317
XceI RCATGY 2 cut(s) 856, 1264
XcmI CCANNNNNNNNNTGG 1 cut(s) 973
XmaJI CCTAGG 1 cut(s) 947
XspI CTAG 4 cut(s) 318, 948, 1352, 1491
Zsp2I ATGCAT 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.