MD15G1330000.v1.1

Regulation of nuclear pre-mRNA domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010009
Physical Location & Seq
Forward (+)
36331364 .. 36337051
5688 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1330000.v1.1.491

Sequence Viewer

Length: 1560 bp
ATGAATAGTGTATTCACAGAGCAGATACTTGCAGACAAGCTCTCCAAGCTCAACAGCACCCAGCAATGCATTGAAACTTTGTCACATTGGTGTATATTTCACCGGAGCAAAGCAGAACTGGTTGTTGCAACATGGGATAAACAATTCCGTAGTGCACAGATGGTTCAGAAAGTTCCTCTCTTGTATCTTGCAAATGACATCCTGCAGAACAGTAAGCGGAAAGGAAATGAATTTGTTACTGAGTTTTGGAAGGTTCTTCCAGCAGCTCTCAAAGTTGTTCACGACGAAGGCGATAATCATGGAAAGAATGTTGTATCTAGATTGGTTGGTATTTGGGAAGAGAGACGAGTATTTGGGTCTCATGCAAGGAGCCTAAAAGAAGTAATGCTTGGGGAAGAAGTGCCTCCACCATTGGAATTCAGTGGCAAAAAGCGCCCTCGTTCAGTCAGAATTGTTAAAAGAGATTCACGCTCTATCAGAACGAAACTTTCTATTGGAGGTCCAGCTGAGAAGATTGTATCCGCATTTCACTTGGTGATCAGTGAACATCCCACTGAAGATGCTGAGATGAGTAAATGCAAGTCTGCAGCTCACCGTGTAAGGAAGTTGGAGAAAGATGTTGACGTTGCCTGTACTAATGCTAAAGATCCAAAGCGGGAAACTTTAGCGAAAGAACTAGAGGAGGAGGAAAATATTTTGAAGCAGTGTATCGATAAACTTAAATCAGTTGAAACCAGTAGAGTAGCACTTTTATCTCAATTAAAAGAAGCTCTGCATGATCAGGAATCTGAACTGGAGAATATTCGAACTCATATGCAGGTAGCACAGGCACAGGTAGAAGAAGCCACCAACATGCGGAAGCGGCTCAATGATGATGATTATGTGTTTAAACCATCTAGTGCGACCAGTCCATCAACTGATGCAAATTCTAAAGGCGGACAAACACCTAGGAAATCAGCTGCAGCCATTGCAGCTTTGGTTGCAGACAAGCTTGCTGCTTCTAGTTCTTCTCAATCGATCATGACTTCAGTTCTTTCAACATTTGCTGCTGAAGAAGCGAAGAATGCTGGTCTGACAAAGCCCTCCACATCTATGCCCCAGAGTTCGGGCACTGATTCAATATCCAAACCTGAGAGGTCCATGCCTGTATCAGATGCCAATGTTTTTATGTCATCACAAGCGCCTTCTGTGCAGCCAAGCCATTCGTACCAATCAGCATTGGTTCCCCAACCGCAGAATCAAGCCCCAACCTCTCAAGGTCAATTTCACATGCTTCCAAACCCACCCTCCCAACAATATTTACAGCCATCAGGAGGGGCCATGAGCCCATATGTTTACGGTAGCATTCCACCTTTGCCTCTAGGGCCACCACCTCGCCCACCTCATATGGTGACTTCAATGGTGCCTTTGGCTCAGCAGCCATTGCAAATGAATCAGCAACCTGTACAAATTAGCCAGCAGCAACCAGTACCCTCAACTCAGCAGCCTCCTCCTGCACCTAGTTTCCGGCCACTTCAGCCACCCAGTATGGTGTATTATACTTATCATCATTCTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CTD_bind

Protein Analysis

520

Amino Acids

57.32

Weight (kDa)

8.95

Isoelectric Point (pI)

62.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CID PF04818 9 - 120 2.9e-35 CID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 808
AccB1I GGYRCC 1 cut(s) 1402
AciI CCGC 7 cut(s) 217, 522, 655, 856, 862, 936, 1232
AclWI GGATC 1 cut(s) 641
AcoI YGGCCR 1 cut(s) 1508
AcsI RAATTY 3 cut(s) 230, 416, 925
AcuI CTGAAG 4 cut(s) 576, 1011, 1071, 1499
AdeI CACNNNGTG 1 cut(s) 535
AfaI GTAC 4 cut(s) 634, 1208, 1446, 1470
AfiI CCNNNNNNNGG 3 cut(s) 855, 1105, 1313
AgsI TTSAA 6 cut(s) 74, 700, 731, 1038, 1119, 1398
AjuI GAANNNNNNNTTGG 2 cut(s) 372, 404
AleI CACNNNNGTG 1 cut(s) 88
AluBI AGCT 9 cut(s) 40, 49, 266, 506, 590, 770, 959, 974, 991
AluI AGCT 9 cut(s) 40, 49, 266, 506, 590, 770, 959, 974, 991
Alw21I GWGCWC 1 cut(s) 157
Alw26I GTCTC 2 cut(s) 337, 363
Alw44I GTGCAC 1 cut(s) 153
AlwI GGATC 1 cut(s) 641
AoxI GGCC 3 cut(s) 1317, 1364, 1508
ApaLI GTGCAC 1 cut(s) 153
ApoI RAATTY 3 cut(s) 230, 416, 925
AspA2I CCTAGG 1 cut(s) 947
AspLEI GCGC 2 cut(s) 435, 1183
AspS9I GGNCC 4 cut(s) 500, 1137, 1317, 1364
AsuHPI GGTGA 4 cut(s) 92, 547, 584, 1402
AsuII TTCGAA 1 cut(s) 805
AvaII GGWCC 2 cut(s) 500, 1137
AvrII CCTAGG 1 cut(s) 947
BaeGI GKGCMC 2 cut(s) 157, 1112
BanI GGYRCC 1 cut(s) 1402
BanII GRGCYC 1 cut(s) 1328
Bbv12I GWGCWC 1 cut(s) 157
BccI CCATC 4 cut(s) 154, 901, 919, 1315
BciVI GTATCC 1 cut(s) 529
BclI TGATCA 2 cut(s) 537, 778
BcoDI GTCTC 2 cut(s) 337, 363
BfaI CTAG 7 cut(s) 318, 677, 897, 948, 1002, 1361, 1500
BfmI CTRYAG 3 cut(s) 203, 585, 960
BfoI RGCGCY 2 cut(s) 436, 1184
BfuAI ACCTGC 1 cut(s) 808
BfuI GTATCC 1 cut(s) 529
BglI GCCNNNNNGGC 1 cut(s) 1363
BlnI CCTAGG 1 cut(s) 947
BlpI GCTNAGC 1 cut(s) 1413
Bme18I GGWCC 2 cut(s) 500, 1137
BmgT120I GGNCC 4 cut(s) 500, 1137, 1317, 1364
BmiI GGNNCC 4 cut(s) 371, 1224, 1318, 1404
BmrI ACTGGG 1 cut(s) 1518
BmsI GCATC 3 cut(s) 550, 910, 1144
BmuI ACTGGG 1 cut(s) 1518
BpmI CTGGAG 1 cut(s) 815
Bpu1102I GCTNAGC 1 cut(s) 1413
Bpu14I TTCGAA 1 cut(s) 805
BpuEI CTTGAG 1 cut(s) 1239
Bsa29I ATCGAT 2 cut(s) 711, 1016
BsaI GGTCTC 1 cut(s) 363
BsaJI CCNNGG 1 cut(s) 947
BsaWI WCCGGW 1 cut(s) 102
BsaXI ACNNNNNCTCC 4 cut(s) 26, 56, 1271, 1301
Bsc4I CCNNNNNNNGG 3 cut(s) 855, 1105, 1313
Bse1I ACTGG 6 cut(s) 123, 735, 798, 906, 1466, 1524
Bse3DI GCAATG 3 cut(s) 71, 966, 1421
BseCI ATCGAT 2 cut(s) 711, 1016
BseDI CCNNGG 1 cut(s) 947
BseGI GGATG 2 cut(s) 198, 547
BseLI CCNNNNNNNGG 3 cut(s) 855, 1105, 1313
BseMI GCAATG 3 cut(s) 71, 966, 1421
BseMII CTCAG 6 cut(s) 231, 498, 555, 1122, 1427, 1493
BseNI ACTGG 6 cut(s) 123, 735, 798, 906, 1466, 1524
BseRI GAGGAG 3 cut(s) 695, 698, 1479
BseSI GKGCMC 2 cut(s) 157, 1112
BseYI CCCAGC 1 cut(s) 60
BsgI GTGCAG 2 cut(s) 1211, 1479
BshFI GGCC 3 cut(s) 1319, 1366, 1510
BshNI GGYRCC 1 cut(s) 1402
BshVI ATCGAT 2 cut(s) 711, 1016
BsiHKAI GWGCWC 1 cut(s) 157
BsiSI CCGG 2 cut(s) 103, 1507
BslI CCNNNNNNNGG 3 cut(s) 855, 1105, 1313
BsmAI GTCTC 2 cut(s) 337, 363
BsmBI CGTCTC 1 cut(s) 337
BsmI GAATGC 2 cut(s) 1069, 1344
BsnI GGCC 3 cut(s) 1319, 1366, 1510
Bso31I GGTCTC 1 cut(s) 363
Bsp119I TTCGAA 1 cut(s) 805
Bsp1286I GDGCHC 3 cut(s) 157, 1112, 1328
Bsp1407I TGTACA 1 cut(s) 1444
Bsp143I GATC 4 cut(s) 537, 646, 778, 1017
Bsp1720I GCTNAGC 1 cut(s) 1413
BspACI CCGC 7 cut(s) 217, 522, 655, 856, 862, 936, 1232
BspANI GGCC 3 cut(s) 1319, 1366, 1510
BspCNI CTCAG 6 cut(s) 232, 499, 556, 1123, 1426, 1492
BspDI ATCGAT 2 cut(s) 711, 1016
BspHI TCATGA 1 cut(s) 1020
BspLI GGNNCC 4 cut(s) 371, 1224, 1318, 1404
BspMAI CTGCAG 3 cut(s) 207, 589, 964
BspMI ACCTGC 1 cut(s) 808
BspPI GGATC 1 cut(s) 641
BspT104I TTCGAA 1 cut(s) 805
BspT107I GGYRCC 1 cut(s) 1402
BspTNI GGTCTC 1 cut(s) 363
BsrDI GCAATG 3 cut(s) 71, 966, 1421
BsrGI TGTACA 1 cut(s) 1444
BsrI ACTGG 6 cut(s) 123, 735, 798, 906, 1466, 1524
BssECI CCNNGG 1 cut(s) 947
BssMI GATC 4 cut(s) 537, 646, 778, 1017
BssT1I CCWWGG 1 cut(s) 947
Bst4CI ACNGT 3 cut(s) 212, 596, 1340
Bst6I CTCTTC 1 cut(s) 333
BstAPI GCANNNNNTGC 2 cut(s) 968, 1423
BstAUI TGTACA 1 cut(s) 1444
BstBI TTCGAA 1 cut(s) 805
BstC8I GCNNGC 2 cut(s) 993, 1457
BstDEI CTNAG 7 cut(s) 240, 507, 564, 1131, 1413, 1479, 1554
BstF5I GGATG 2 cut(s) 198, 547
BstH2I RGCGCY 2 cut(s) 436, 1184
BstHHI GCGC 2 cut(s) 435, 1183
BstKTI GATC 4 cut(s) 540, 649, 781, 1020
BstMAI GTCTC 2 cut(s) 337, 363
BstMBI GATC 4 cut(s) 537, 646, 778, 1017
BstNSI RCATGY 2 cut(s) 856, 1273
BstSFI CTRYAG 3 cut(s) 203, 585, 960
BstSLI GKGCMC 2 cut(s) 157, 1112
BstX2I RGATCY 1 cut(s) 646
BstYI RGATCY 1 cut(s) 646
Bsu15I ATCGAT 2 cut(s) 711, 1016
BsuI GTATCC 1 cut(s) 529
BsuRI GGCC 3 cut(s) 1319, 1366, 1510
BsuTUI ATCGAT 2 cut(s) 711, 1016
BtsCI GGATG 2 cut(s) 198, 547
BtsI GCAGTG 1 cut(s) 710
BtsIMutI CAGTG 5 cut(s) 427, 547, 552, 710, 1110
BveI ACCTGC 1 cut(s) 808
Cac8I GCNNGC 2 cut(s) 993, 1457
CciI TCATGA 1 cut(s) 1020
CfoI GCGC 2 cut(s) 435, 1183
Cfr13I GGNCC 4 cut(s) 500, 1137, 1317, 1364
ClaI ATCGAT 2 cut(s) 711, 1016
Csp6I GTAC 4 cut(s) 633, 1207, 1445, 1469
CviAII CATG 9 cut(s) 132, 299, 362, 776, 853, 1021, 1141, 1270, 1321
CviQI GTAC 4 cut(s) 633, 1207, 1445, 1469
DdeI CTNAG 7 cut(s) 240, 507, 564, 1131, 1413, 1479, 1554
DpnI GATC 4 cut(s) 539, 648, 780, 1019
DpnII GATC 4 cut(s) 537, 646, 778, 1017
DraI TTTAAA 1 cut(s) 889
DraIII CACNNNGTG 1 cut(s) 535
EaeI YGGCCR 1 cut(s) 1508
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
EciI GGCGGA 1 cut(s) 951
Eco130I CCWWGG 1 cut(s) 947
Eco24I GRGCYC 1 cut(s) 1328
Eco31I GGTCTC 1 cut(s) 363
Eco47I GGWCC 2 cut(s) 500, 1137
Eco57I CTGAAG 4 cut(s) 576, 1011, 1071, 1499
EcoRI GAATTC 1 cut(s) 416
EcoT14I CCWWGG 1 cut(s) 947
EcoT22I ATGCAT 1 cut(s) 71
EcoT38I GRGCYC 1 cut(s) 1328
ErhI CCWWGG 1 cut(s) 947
Esp3I CGTCTC 1 cut(s) 337
FaeI CATG 9 cut(s) 135, 302, 365, 779, 856, 1024, 1144, 1273, 1324
FalI AAGNNNNNCTT 2 cut(s) 372, 404
FatI CATG 9 cut(s) 131, 298, 361, 775, 852, 1020, 1140, 1269, 1320
FauI CCCGC 1 cut(s) 648
FauNDI CATATG 3 cut(s) 813, 1330, 1386
FbaI TGATCA 2 cut(s) 537, 778
FokI GGATG 2 cut(s) 185, 534
FriOI GRGCYC 1 cut(s) 1328
FspBI CTAG 7 cut(s) 318, 677, 897, 948, 1002, 1361, 1500
GlaI GCGC 2 cut(s) 434, 1182
GsaI CCCAGC 1 cut(s) 64
GsuI CTGGAG 1 cut(s) 815
HaeII RGCGCY 2 cut(s) 436, 1184
HaeIII GGCC 3 cut(s) 1319, 1366, 1510
HapII CCGG 2 cut(s) 103, 1507
HhaI GCGC 2 cut(s) 435, 1183
Hin1II CATG 9 cut(s) 135, 302, 365, 779, 856, 1024, 1144, 1273, 1324
Hin6I GCGC 2 cut(s) 433, 1181
HinP1I GCGC 2 cut(s) 433, 1181
HincII GTYRAC 1 cut(s) 622
HindII GTYRAC 1 cut(s) 622
HindIII AAGCTT 1 cut(s) 989
HinfI GANTC 5 cut(s) 464, 785, 1115, 1237, 1432
HpaII CCGG 2 cut(s) 103, 1507
HphI GGTGA 4 cut(s) 92, 547, 584, 1402
Hpy166II GTNNAC 5 cut(s) 155, 280, 545, 622, 1336
Hpy188I TCNGA 6 cut(s) 168, 449, 479, 790, 1074, 1153
Hpy188III TCNNGA 5 cut(s) 281, 318, 782, 1021, 1311
Hpy8I GTNNAC 5 cut(s) 155, 280, 545, 622, 1336
Hpy99I CGWCG 1 cut(s) 287
HpyAV CCTTC 3 cut(s) 244, 281, 1194
HpyCH4III ACNGT 3 cut(s) 212, 596, 1340
HpyCH4IV ACGT 1 cut(s) 624
HpyF3I CTNAG 7 cut(s) 240, 507, 564, 1131, 1413, 1479, 1554
HpySE526I ACGT 1 cut(s) 624
Hsp92II CATG 9 cut(s) 135, 302, 365, 779, 856, 1024, 1144, 1273, 1324
HspAI GCGC 2 cut(s) 433, 1181
Ksp22I TGATCA 2 cut(s) 537, 778
Kzo9I GATC 4 cut(s) 537, 646, 778, 1017
LmnI GCTCC 2 cut(s) 105, 369
LweI GCATC 3 cut(s) 550, 910, 1144
MaeI CTAG 7 cut(s) 318, 677, 897, 948, 1002, 1361, 1500
MaeII ACGT 1 cut(s) 624
MaeIII GTNAC 3 cut(s) 81, 235, 1390
MalI GATC 4 cut(s) 539, 648, 780, 1019
MboI GATC 4 cut(s) 537, 646, 778, 1017
MboII GAAGA 9 cut(s) 248, 350, 407, 523, 569, 851, 999, 1064, 1072
MflI RGATCY 1 cut(s) 646
MhlI GDGCHC 3 cut(s) 157, 1112, 1328
MluCI AATT 8 cut(s) 143, 230, 416, 450, 758, 925, 1262, 1449
MmeI TCCRAC 1 cut(s) 588
Mph1103I ATGCAT 1 cut(s) 71
MseI TTAA 4 cut(s) 456, 720, 761, 888
MslI CAYNNNNRTG 3 cut(s) 88, 851, 1091
MspA1I CMGCKG 2 cut(s) 506, 959
MspI CCGG 2 cut(s) 103, 1507
MssI GTTTAAAC 1 cut(s) 889
Mva1269I GAATGC 2 cut(s) 1069, 1344
NdeI CATATG 3 cut(s) 813, 1330, 1386
NdeII GATC 4 cut(s) 537, 646, 778, 1017
NlaIII CATG 9 cut(s) 135, 302, 365, 779, 856, 1024, 1144, 1273, 1324
NlaIV GGNNCC 4 cut(s) 371, 1224, 1318, 1404
NmuCI GTSAC 2 cut(s) 81, 1390
NsiI ATGCAT 1 cut(s) 71
NspI RCATGY 2 cut(s) 856, 1273
NspV TTCGAA 1 cut(s) 805
OliI CACNNNNGTG 1 cut(s) 88
PagI TCATGA 1 cut(s) 1020
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PctI GAATGC 2 cut(s) 1069, 1344
PfeI GAWTC 5 cut(s) 464, 785, 1115, 1237, 1432
PmeI GTTTAAAC 1 cut(s) 889
PspFI CCCAGC 1 cut(s) 60
PspN4I GGNNCC 4 cut(s) 371, 1224, 1318, 1404
PspPI GGNCC 4 cut(s) 500, 1137, 1317, 1364
PstI CTGCAG 3 cut(s) 207, 589, 964
PsuI RGATCY 1 cut(s) 646
PvuII CAGCTG 2 cut(s) 506, 959
RsaI GTAC 4 cut(s) 634, 1208, 1446, 1470
RsaNI GTAC 4 cut(s) 633, 1207, 1445, 1469
RseI CAYNNNNRTG 3 cut(s) 88, 851, 1091
SaqAI TTAA 4 cut(s) 456, 720, 761, 888
Sau3AI GATC 4 cut(s) 537, 646, 778, 1017
Sau96I GGNCC 4 cut(s) 500, 1137, 1317, 1364
SduI GDGCHC 3 cut(s) 157, 1112, 1328
SfaNI GCATC 3 cut(s) 550, 910, 1144
SfcI CTRYAG 3 cut(s) 203, 585, 960
SfuI TTCGAA 1 cut(s) 805
SinI GGWCC 2 cut(s) 500, 1137
SmiMI CAYNNNNRTG 3 cut(s) 88, 851, 1091
SmlI CTYRAG 1 cut(s) 1254
SmoI CTYRAG 1 cut(s) 1254
Sse9I AATT 8 cut(s) 143, 230, 416, 450, 758, 925, 1262, 1449
SsiI CCGC 7 cut(s) 217, 522, 655, 856, 862, 936, 1232
SspI AATATT 3 cut(s) 694, 802, 1298
SspMI CTAG 7 cut(s) 318, 677, 897, 948, 1002, 1361, 1500
StyI CCWWGG 1 cut(s) 947
TaaI ACNGT 3 cut(s) 212, 596, 1340
TaiI ACGT 1 cut(s) 627
TaqI TCGA 3 cut(s) 711, 805, 1016
TasI AATT 8 cut(s) 143, 230, 416, 450, 758, 925, 1262, 1449
TatI WGTACW 2 cut(s) 632, 1444
TauI GCSGC 1 cut(s) 865
TfiI GAWTC 5 cut(s) 464, 785, 1115, 1237, 1432
Tru1I TTAA 4 cut(s) 456, 720, 761, 888
Tru9I TTAA 4 cut(s) 456, 720, 761, 888
TscAI CASTG 5 cut(s) 427, 547, 559, 710, 1117
TseFI GTSAC 2 cut(s) 81, 1390
Tsp45I GTSAC 2 cut(s) 81, 1390
TspDTI ATGAA 3 cut(s) 17, 243, 1445
TspGWI ACGGA 1 cut(s) 137
TspRI CASTG 5 cut(s) 427, 547, 559, 710, 1117
VneI GTGCAC 1 cut(s) 153
VpaK11BI GGWCC 2 cut(s) 500, 1137
XapI RAATTY 3 cut(s) 230, 416, 925
XbaI TCTAGA 1 cut(s) 317
XceI RCATGY 2 cut(s) 856, 1273
XcmI CCANNNNNNNNNTGG 1 cut(s) 973
XmaJI CCTAGG 1 cut(s) 947
XspI CTAG 7 cut(s) 318, 677, 897, 948, 1002, 1361, 1500
Zsp2I ATGCAT 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.