AT5G66110

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004026
Physical Location & Seq
Forward (+)
26430071 .. 26431351
1281 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66110.2

Sequence Viewer

Length: 444 bp
ATGGGCTTCCGAGACATTTGTTACCGGAAACATCACAAAAAGCTTAAGCAGTTCCAGAAGGTGGAGATAAAAGTGAAGATGGACTGCGAAGGGTGCGAGAGACGGGTGAGGAAGTCGGTGGAAGGCATGAAAGGAGTCAGCAAAGTCACCGTCGATCCTAAACAGAGCAAACTCACGGTCGAGGGATTCGTCCAACCGAGCAAGGTGGTGCATCGGGTGATGCATCGGACAGGGAAGAAGGCGGAGCTGTGGCCTTACGTACCTTACGAGGTGGTGCCACACCCTTACGCACCTGGTGCCTACGACAAGAAGGCCCCTCCTGGTTACGTTCGTAATGCACTCGCCGACCCTCTAGTGGCCCCGCTTGCTCGTGCCAGCTCCTTCGAGGTCAAATACACTTCTGCTTTCAGCGATGACAATCCCAACGCTTGTACCATCATGTGA

Protein Analysis

147

Amino Acids

16.65

Weight (kDa)

9.65

Isoelectric Point (pI)

37.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 1 - 54 1.9e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 274, 296
AccB7I CCANNNNNTGG 1 cut(s) 61
AciI CCGC 2 cut(s) 242, 362
AclWI GGATC 1 cut(s) 149
AdeI CACNNNGTG 1 cut(s) 296
AfaI GTAC 2 cut(s) 261, 433
AfiI CCNNNNNNNGG 2 cut(s) 61, 355
AflII CTTAAG 1 cut(s) 44
AjnI CCWGG 2 cut(s) 292, 319
AluBI AGCT 3 cut(s) 43, 247, 378
AluI AGCT 3 cut(s) 43, 247, 378
Alw26I GTCTC 2 cut(s) 6, 94
AlwI GGATC 1 cut(s) 149
AoxI GGCC 3 cut(s) 251, 312, 357
ArsI GACNNNNNNTTYG 4 cut(s) 135, 162, 167, 194
AspS9I GGNCC 2 cut(s) 313, 358
AsuHPI GGTGA 3 cut(s) 118, 139, 229
BaeI ACNNNNGTAYC 1 cut(s) 415
BanI GGYRCC 2 cut(s) 274, 296
BauI CACGAG 1 cut(s) 369
BccI CCATC 2 cut(s) 73, 443
BciT130I CCWGG 2 cut(s) 294, 321
BcoDI GTCTC 2 cut(s) 6, 94
BfaI CTAG 1 cut(s) 353
BfrI CTTAAG 1 cut(s) 44
Bme1390I CCNGG 2 cut(s) 294, 321
BmgT120I GGNCC 2 cut(s) 313, 358
BmiI GGNNCC 4 cut(s) 276, 298, 315, 360
BmrFI CCNGG 2 cut(s) 294, 321
BmsI GCATC 3 cut(s) 210, 220, 232
BsaAI YACGTR 1 cut(s) 259
BsaBI GATNNNNATC 1 cut(s) 417
BsaWI WCCGGW 1 cut(s) 24
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 355
Bse8I GATNNNNATC 1 cut(s) 417
BseBI CCWGG 2 cut(s) 294, 321
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 2 cut(s) 61, 355
Bsh1285I CGRYCG 1 cut(s) 180
BshFI GGCC 3 cut(s) 253, 314, 359
BshNI GGYRCC 2 cut(s) 274, 296
BsiEI CGRYCG 1 cut(s) 180
BsiSI CCGG 1 cut(s) 25
BslI CCNNNNNNNGG 2 cut(s) 61, 355
BsmAI GTCTC 2 cut(s) 6, 94
BsmBI CGTCTC 1 cut(s) 94
BsnI GGCC 3 cut(s) 253, 314, 359
Bsp143I GATC 1 cut(s) 154
BspACI CCGC 2 cut(s) 242, 362
BspANI GGCC 3 cut(s) 253, 314, 359
BspLI GGNNCC 4 cut(s) 276, 298, 315, 360
BspPI GGATC 1 cut(s) 149
BspT107I GGYRCC 2 cut(s) 274, 296
BspTI CTTAAG 1 cut(s) 44
BssMI GATC 1 cut(s) 154
BssSI CACGAG 1 cut(s) 369
Bst2BI CACGAG 1 cut(s) 369
Bst2UI CCWGG 2 cut(s) 294, 321
Bst4CI ACNGT 2 cut(s) 151, 178
BstAFI CTTAAG 1 cut(s) 44
BstAPI GCANNNNNTGC 1 cut(s) 296
BstBAI YACGTR 1 cut(s) 259
BstC8I GCNNGC 2 cut(s) 366, 376
BstKTI GATC 1 cut(s) 157
BstMAI GTCTC 2 cut(s) 6, 94
BstMBI GATC 1 cut(s) 154
BstMCI CGRYCG 1 cut(s) 180
BstMWI GCNNNNNNNGC 3 cut(s) 93, 296, 365
BstNI CCWGG 2 cut(s) 294, 321
BstSCI CCNGG 2 cut(s) 292, 319
BstSNI TACGTA 1 cut(s) 259
BsuRI GGCC 3 cut(s) 253, 314, 359
BtgZI GCGATG 1 cut(s) 426
Cac8I GCNNGC 2 cut(s) 366, 376
Cfr13I GGNCC 2 cut(s) 313, 358
CsiI ACCWGGT 1 cut(s) 292
Csp6I GTAC 2 cut(s) 260, 432
CviAII CATG 2 cut(s) 127, 439
CviJI RGCY 7 cut(s) 6, 43, 247, 253, 314, 359, 378
CviKI_1 RGCY 7 cut(s) 6, 43, 247, 253, 314, 359, 378
CviQI GTAC 2 cut(s) 260, 432
DpnI GATC 1 cut(s) 156
DpnII GATC 1 cut(s) 154
DraIII CACNNNGTG 1 cut(s) 296
EciI GGCGGA 1 cut(s) 257
Eco105I TACGTA 1 cut(s) 259
EcoO109I RGGNCCY 1 cut(s) 313
EcoRII CCWGG 2 cut(s) 292, 319
EcoT22I ATGCAT 1 cut(s) 225
Esp3I CGTCTC 1 cut(s) 94
FaeI CATG 2 cut(s) 130, 442
FaiI YATR 2 cut(s) 128, 440
FatI CATG 2 cut(s) 126, 438
FauI CCCGC 1 cut(s) 369
FspBI CTAG 1 cut(s) 353
HaeIII GGCC 3 cut(s) 253, 314, 359
HapII CCGG 1 cut(s) 25
Hin1II CATG 2 cut(s) 130, 442
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 2 cut(s) 135, 186
HpaII CCGG 1 cut(s) 25
HphI GGTGA 3 cut(s) 118, 139, 229
Hpy188I TCNGA 2 cut(s) 11, 228
Hpy188III TCNNGA 1 cut(s) 55
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 6 cut(s) 52, 83, 116, 232, 304, 391
HpyCH4III ACNGT 2 cut(s) 151, 178
HpyCH4IV ACGT 2 cut(s) 258, 327
HpyCH4V TGCA 3 cut(s) 211, 223, 338
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 296, 365
HpySE526I ACGT 2 cut(s) 258, 327
Hsp92II CATG 2 cut(s) 130, 442
Kzo9I GATC 1 cut(s) 154
LmnI GCTCC 2 cut(s) 244, 383
LpnPI CCDG 8 cut(s) 38, 68, 216, 279, 306, 306, 333, 388
LweI GCATC 3 cut(s) 210, 220, 232
MabI ACCWGGT 1 cut(s) 292
MaeI CTAG 1 cut(s) 353
MaeII ACGT 2 cut(s) 258, 327
MaeIII GTNAC 3 cut(s) 20, 145, 323
MalI GATC 1 cut(s) 156
MboI GATC 1 cut(s) 154
MboII GAAGA 2 cut(s) 88, 247
MlyI GAGTC 1 cut(s) 144
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 6 cut(s) 102, 175, 262, 327, 360, 379
Mph1103I ATGCAT 1 cut(s) 225
MseI TTAA 1 cut(s) 45
MspCI CTTAAG 1 cut(s) 44
MspI CCGG 1 cut(s) 25
MspR9I CCNGG 2 cut(s) 294, 321
MvaI CCWGG 2 cut(s) 294, 321
MwoI GCNNNNNNNGC 3 cut(s) 93, 296, 365
NdeII GATC 1 cut(s) 154
NlaIII CATG 2 cut(s) 130, 442
NlaIV GGNNCC 4 cut(s) 276, 298, 315, 360
NmuCI GTSAC 1 cut(s) 145
NsiI ATGCAT 1 cut(s) 225
PcsI WCGNNNNNNNCGW 2 cut(s) 186, 264
PfeI GAWTC 1 cut(s) 186
PflMI CCANNNNNTGG 1 cut(s) 61
PleI GAGTC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 143
Ppu21I YACGTR 1 cut(s) 259
Psp6I CCWGG 2 cut(s) 292, 319
PspGI CCWGG 2 cut(s) 292, 319
PspN4I GGNNCC 4 cut(s) 276, 298, 315, 360
PspPI GGNCC 2 cut(s) 313, 358
RsaI GTAC 2 cut(s) 261, 433
RsaNI GTAC 2 cut(s) 260, 432
SaqAI TTAA 1 cut(s) 45
Sau3AI GATC 1 cut(s) 154
Sau96I GGNCC 2 cut(s) 313, 358
SchI GAGTC 1 cut(s) 144
ScrFI CCNGG 2 cut(s) 294, 321
SexAI ACCWGGT 1 cut(s) 292
SfaNI GCATC 3 cut(s) 210, 220, 232
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
SnaBI TACGTA 1 cut(s) 259
SsiI CCGC 2 cut(s) 242, 362
SspMI CTAG 1 cut(s) 353
StyD4I CCNGG 2 cut(s) 292, 319
TaaI ACNGT 2 cut(s) 151, 178
TaiI ACGT 2 cut(s) 261, 330
TaqI TCGA 3 cut(s) 153, 180, 384
TfiI GAWTC 1 cut(s) 186
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TseFI GTSAC 1 cut(s) 145
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 1 cut(s) 143
Van91I CCANNNNNTGG 1 cut(s) 61
Vha464I CTTAAG 1 cut(s) 44
XspI CTAG 1 cut(s) 353
Zsp2I ATGCAT 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.