Rw0G006000

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00235
OG0004026
Physical Location & Seq
Reverse (-)
23648 .. 24268
621 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G006000.1

Sequence Viewer

Length: 474 bp
ATGGGTTTTCTCAGTAAAGTCTCCGAGCTCCGTGCTAGGGCTCGGAGTCATAAAAAGCTCAAGAAGAACAAGCAGCTACAGACTGTGGAGATAAAAGTGAAGATGGACTGCGAAGGTTGCGAGAGAAGAGTGAAGAAATCAGTGGAAGGCATGAAAGGAGTCACTAGTGTTGAAGTGGATCCCAAGCAAAGCAAACTCACTGTTATTGGCTATGTGGACCCCAACAAGGTGTTGCATCGTGTCAGGCATCGAACGGGGAAGAAGGCGGATCTGTGGCCGTATGTACCATACGACGTCGTCGAGCATCCTTGGGCTCCCGGGGCTTATGATAGGAAGGCACCACCTGGGTACGTGAGGAACGTGCTGAATGACCCTGACCCTCAGGCTTCTGCTCTCGCACGTGCCAACTCCACCGAGGTGAAGTATACGACGGCGTTTAGTGACGAAAATCCAAATGCTTGTGTGGTTATGTAG

Protein Analysis

157

Amino Acids

17.68

Weight (kDa)

9.66

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 32 - 87 6.7e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 297
AccB1I GGYRCC 1 cut(s) 337
AccI GTMKAC 1 cut(s) 425
AciI CCGC 1 cut(s) 266
AclWI GGATC 3 cut(s) 173, 186, 276
AcoI YGGCCR 1 cut(s) 275
AcvI CACGTG 1 cut(s) 401
AcyI GRCGYC 1 cut(s) 294
AfaI GTAC 2 cut(s) 285, 350
AfiI CCNNNNNNNGG 2 cut(s) 37, 226
AgsI TTSAA 1 cut(s) 173
AhlI ACTAGT 1 cut(s) 164
AjnI CCWGG 1 cut(s) 343
AleI CACNNNNGTG 1 cut(s) 416
AluBI AGCT 3 cut(s) 28, 58, 76
AluI AGCT 3 cut(s) 28, 58, 76
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 1 cut(s) 25
AlwI GGATC 3 cut(s) 173, 186, 276
Ama87I CYCGRG 1 cut(s) 317
AoxI GGCC 1 cut(s) 275
ApeKI GCWGC 1 cut(s) 73
AspS9I GGNCC 1 cut(s) 217
AsuC2I CCSGG 2 cut(s) 318, 319
AsuHPI GGTGA 1 cut(s) 430
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 1 cut(s) 217
AxyI CCTNAGG 1 cut(s) 381
BamHI GGATCC 1 cut(s) 178
BanI GGYRCC 1 cut(s) 337
BanII GRGCYC 3 cut(s) 30, 43, 316
BbrPI CACGTG 1 cut(s) 401
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 1 cut(s) 85
BccI CCATC 1 cut(s) 97
BceAI ACGGC 2 cut(s) 262, 447
BcgI CGANNNNNNTGC 2 cut(s) 14, 48
BciT130I CCWGG 1 cut(s) 345
BcnI CCSGG 2 cut(s) 318, 319
BcoDI GTCTC 1 cut(s) 25
BcuI ACTAGT 1 cut(s) 164
BfaI CTAG 2 cut(s) 36, 165
BfmI CTRYAG 1 cut(s) 77
BisI GCNGC 1 cut(s) 74
BlsI GCNGC 1 cut(s) 75
Bme1390I CCNGG 3 cut(s) 318, 319, 345
Bme18I GGWCC 1 cut(s) 217
BmeT110I CYCGRG 1 cut(s) 317
BmgT120I GGNCC 1 cut(s) 217
BmiI GGNNCC 4 cut(s) 180, 219, 315, 339
BmrFI CCNGG 3 cut(s) 318, 319, 345
BmsI GCATC 3 cut(s) 244, 256, 313
BpuEI CTTGAG 1 cut(s) 44
BpuMI CCSGG 2 cut(s) 318, 319
BsaAI YACGTR 2 cut(s) 352, 401
BsaHI GRCGYC 1 cut(s) 294
BsaJI CCNNGG 5 cut(s) 308, 317, 318, 344, 414
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 226
Bse21I CCTNAGG 1 cut(s) 381
BseBI CCWGG 1 cut(s) 345
BseDI CCNNGG 5 cut(s) 308, 317, 318, 344, 414
BseGI GGATG 1 cut(s) 304
BseLI CCNNNNNNNGG 2 cut(s) 37, 226
BseMII CTCAG 2 cut(s) 25, 395
BseXI GCAGC 1 cut(s) 85
BshFI GGCC 1 cut(s) 277
BshNI GGYRCC 1 cut(s) 337
BsiHKAI GWGCWC 1 cut(s) 30
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 1 cut(s) 318
BslI CCNNNNNNNGG 2 cut(s) 37, 226
BsmAI GTCTC 1 cut(s) 25
BsnI GGCC 1 cut(s) 277
BsoBI CYCGRG 1 cut(s) 317
Bsp1286I GDGCHC 3 cut(s) 30, 43, 316
Bsp143I GATC 2 cut(s) 178, 268
BspACI CCGC 1 cut(s) 266
BspANI GGCC 1 cut(s) 277
BspCNI CTCAG 2 cut(s) 24, 394
BspLI GGNNCC 4 cut(s) 180, 219, 315, 339
BspPI GGATC 3 cut(s) 173, 186, 276
BspT107I GGYRCC 1 cut(s) 337
BssECI CCNNGG 5 cut(s) 308, 317, 318, 344, 414
BssMI GATC 2 cut(s) 178, 268
BssNAI GTATAC 1 cut(s) 426
BssNI GRCGYC 1 cut(s) 294
BssT1I CCWWGG 1 cut(s) 308
Bst1107I GTATAC 1 cut(s) 426
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 2 cut(s) 85, 202
Bst6I CTCTTC 1 cut(s) 121
BstACI GRCGYC 1 cut(s) 294
BstBAI YACGTR 2 cut(s) 352, 401
BstDEI CTNAG 2 cut(s) 11, 381
BstF5I GGATG 1 cut(s) 304
BstKTI GATC 2 cut(s) 181, 271
BstMAI GTCTC 1 cut(s) 25
BstMBI GATC 2 cut(s) 178, 268
BstMWI GCNNNNNNNGC 2 cut(s) 117, 320
BstNI CCWGG 1 cut(s) 345
BstSCI CCNGG 3 cut(s) 316, 317, 343
BstSFI CTRYAG 1 cut(s) 77
BstV1I GCAGC 1 cut(s) 85
BstX2I RGATCY 2 cut(s) 178, 268
BstYI RGATCY 2 cut(s) 178, 268
BstZ17I GTATAC 1 cut(s) 426
Bsu36I CCTNAGG 1 cut(s) 381
BsuRI GGCC 1 cut(s) 277
BtsCI GGATG 1 cut(s) 304
BtsIMutI CAGTG 2 cut(s) 147, 198
Cfr13I GGNCC 1 cut(s) 217
Cfr9I CCCGGG 1 cut(s) 317
Csp6I GTAC 2 cut(s) 284, 349
CviAII CATG 1 cut(s) 151
CviJI RGCY 9 cut(s) 28, 41, 58, 76, 210, 277, 314, 323, 386
CviKI_1 RGCY 9 cut(s) 28, 41, 58, 76, 210, 277, 314, 323, 386
CviQI GTAC 2 cut(s) 284, 349
DdeI CTNAG 2 cut(s) 11, 381
DpnI GATC 2 cut(s) 180, 270
DpnII GATC 2 cut(s) 178, 268
EaeI YGGCCR 1 cut(s) 275
Eam1104I CTCTTC 1 cut(s) 121
EarI CTCTTC 1 cut(s) 121
EciI GGCGGA 1 cut(s) 281
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 308
Eco24I GRGCYC 3 cut(s) 30, 43, 316
Eco47I GGWCC 1 cut(s) 217
Eco53kI GAGCTC 1 cut(s) 28
Eco72I CACGTG 1 cut(s) 401
Eco81I CCTNAGG 1 cut(s) 381
Eco88I CYCGRG 1 cut(s) 317
EcoICRI GAGCTC 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 343
EcoT14I CCWWGG 1 cut(s) 308
EcoT38I GRGCYC 3 cut(s) 30, 43, 316
ErhI CCWWGG 1 cut(s) 308
FaeI CATG 1 cut(s) 154
FaiI YATR 8 cut(s) 51, 152, 213, 282, 289, 327, 426, 470
FatI CATG 1 cut(s) 150
FblI GTMKAC 1 cut(s) 425
Fnu4HI GCNGC 1 cut(s) 74
FokI GGATG 1 cut(s) 291
FriOI GRGCYC 3 cut(s) 30, 43, 316
Fsp4HI GCNGC 1 cut(s) 74
FspBI CTAG 2 cut(s) 36, 165
GluI GCNGC 1 cut(s) 74
HaeIII GGCC 1 cut(s) 277
HapII CCGG 1 cut(s) 318
Hin1I GRCGYC 1 cut(s) 294
Hin1II CATG 1 cut(s) 154
HinfI GANTC 2 cut(s) 46, 159
HpaII CCGG 1 cut(s) 318
HphI GGTGA 1 cut(s) 430
Hpy166II GTNNAC 2 cut(s) 217, 426
Hpy188I TCNGA 2 cut(s) 25, 45
Hpy188III TCNNGA 1 cut(s) 61
Hpy8I GTNNAC 2 cut(s) 217, 426
Hpy99I CGWCG 4 cut(s) 296, 299, 302, 433
HpyAV CCTTC 4 cut(s) 107, 140, 256, 328
HpyCH4III ACNGT 2 cut(s) 85, 202
HpyCH4IV ACGT 4 cut(s) 294, 351, 360, 400
HpyCH4V TGCA 1 cut(s) 235
HpyF10VI GCNNNNNNNGC 2 cut(s) 117, 320
HpyF3I CTNAG 2 cut(s) 11, 381
HpySE526I ACGT 4 cut(s) 294, 351, 360, 400
Hsp92I GRCGYC 1 cut(s) 294
Hsp92II CATG 1 cut(s) 154
Kzo9I GATC 2 cut(s) 178, 268
LmnI GCTCC 2 cut(s) 33, 319
LpnPI CCDG 6 cut(s) 229, 330, 331, 357, 368, 387
Lsp1109I GCAGC 1 cut(s) 85
LweI GCATC 3 cut(s) 244, 256, 313
MaeI CTAG 2 cut(s) 36, 165
MaeII ACGT 4 cut(s) 294, 351, 360, 400
MaeIII GTNAC 2 cut(s) 160, 440
MalI GATC 2 cut(s) 180, 270
MboI GATC 2 cut(s) 178, 268
MboII GAAGA 5 cut(s) 76, 112, 138, 145, 271
MflI RGATCY 2 cut(s) 178, 268
MhlI GDGCHC 3 cut(s) 30, 43, 316
MlyI GAGTC 2 cut(s) 55, 168
MnlI CCTC 3 cut(s) 348, 390, 409
MslI CAYNNNNRTG 1 cut(s) 416
MspI CCGG 1 cut(s) 318
MspR9I CCNGG 3 cut(s) 318, 319, 345
MvaI CCWGG 1 cut(s) 345
MwoI GCNNNNNNNGC 2 cut(s) 117, 320
NciI CCSGG 2 cut(s) 318, 319
NdeII GATC 2 cut(s) 178, 268
NlaIII CATG 1 cut(s) 154
NlaIV GGNNCC 4 cut(s) 180, 219, 315, 339
NmuCI GTSAC 2 cut(s) 160, 440
OliI CACNNNNGTG 1 cut(s) 416
PcsI WCGNNNNNNNCGW 2 cut(s) 297, 357
PflFI GACNNNGTC 1 cut(s) 296
PkrI GCNGC 1 cut(s) 75
PleI GAGTC 2 cut(s) 54, 167
PmaCI CACGTG 1 cut(s) 401
PmlI CACGTG 1 cut(s) 401
PpsI GAGTC 2 cut(s) 54, 167
Ppu21I YACGTR 2 cut(s) 352, 401
Psp124BI GAGCTC 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 343
PspCI CACGTG 1 cut(s) 401
PspGI CCWGG 1 cut(s) 343
PspN4I GGNNCC 4 cut(s) 180, 219, 315, 339
PspPI GGNCC 1 cut(s) 217
PsuI RGATCY 2 cut(s) 178, 268
PsyI GACNNNGTC 1 cut(s) 296
RsaI GTAC 2 cut(s) 285, 350
RsaNI GTAC 2 cut(s) 284, 349
RseI CAYNNNNRTG 1 cut(s) 416
SacI GAGCTC 1 cut(s) 30
SatI GCNGC 1 cut(s) 74
Sau3AI GATC 2 cut(s) 178, 268
Sau96I GGNCC 1 cut(s) 217
SchI GAGTC 2 cut(s) 55, 168
ScrFI CCNGG 3 cut(s) 318, 319, 345
SduI GDGCHC 3 cut(s) 30, 43, 316
SfaNI GCATC 3 cut(s) 244, 256, 313
SfcI CTRYAG 1 cut(s) 77
SinI GGWCC 1 cut(s) 217
SmaI CCCGGG 1 cut(s) 319
SmiMI CAYNNNNRTG 1 cut(s) 416
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
SpeI ACTAGT 1 cut(s) 164
SsiI CCGC 1 cut(s) 266
SspMI CTAG 2 cut(s) 36, 165
SstI GAGCTC 1 cut(s) 30
StyD4I CCNGG 3 cut(s) 316, 317, 343
StyI CCWWGG 1 cut(s) 308
TaaI ACNGT 2 cut(s) 85, 202
TaiI ACGT 4 cut(s) 297, 354, 363, 403
TaqI TCGA 2 cut(s) 250, 300
TscAI CASTG 2 cut(s) 147, 205
TseFI GTSAC 2 cut(s) 160, 440
TseI GCWGC 1 cut(s) 73
Tsp45I GTSAC 2 cut(s) 160, 440
TspDTI ATGAA 1 cut(s) 167
TspGWI ACGGA 1 cut(s) 20
TspMI CCCGGG 1 cut(s) 317
TspRI CASTG 2 cut(s) 147, 205
Tth111I GACNNNGTC 1 cut(s) 296
VpaK11BI GGWCC 1 cut(s) 217
XmaI CCCGGG 1 cut(s) 317
XmiI GTMKAC 1 cut(s) 425
XspI CTAG 2 cut(s) 36, 165
ZraI GACGTC 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.