MD02G1126800.v1.1

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0004026
Physical Location & Seq
Reverse (-)
10252106 .. 10252471
366 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1126800.v1.1.491

Sequence Viewer

Length: 366 bp
ATGGACTGTGAAGGGTGCGAGAGAAGAGTGAAGAAATCCGTGCAAGGCATGAAAGGAGTCACGGAGGTTGAGGTGGACCCCAAGCAGAGCAAGCTCACTGTGATTGGCTATGTGGACCCCGATAAAGTGTTGCATCGTGTCAGGCATCGAACGGGGAAGAAGGCTGAGCTCTGGCCTTATGTGCCGTACGATGTCGTTCCGCATCCCTACGCCCCGGAGGCATATGACAGGAAGGCCCCACCTGGGTACGTGCGGAACGTGCTGGAGGATCCGGAGGCTTCGGCTCTCGCACGTGCAAGCTCCACTGAAGTGAAGTATACGACGGCGTTTAGTGACGAGAATCCAAATGCGTGTGTGGTCATGTAG

Protein Analysis

122

Amino Acids

13.56

Weight (kDa)

6.82

Isoelectric Point (pI)

39.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 1 - 53 5.6e-13 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 317
AccIII TCCGGA 1 cut(s) 271
AciI CCGC 2 cut(s) 200, 253
AclWI GGATC 2 cut(s) 263, 276
AcuI CTGAAG 1 cut(s) 327
AcvI CACGTG 1 cut(s) 293
AfaI GTAC 2 cut(s) 188, 248
AfiI CCNNNNNNNGG 1 cut(s) 243
AjnI CCWGG 1 cut(s) 241
AleI CACNNNNGTG 1 cut(s) 308
AluBI AGCT 3 cut(s) 94, 169, 300
AluI AGCT 3 cut(s) 94, 169, 300
Alw21I GWGCWC 1 cut(s) 171
AlwI GGATC 2 cut(s) 263, 276
Aor13HI TCCGGA 1 cut(s) 271
AoxI GGCC 2 cut(s) 173, 234
AspS9I GGNCC 3 cut(s) 76, 115, 235
AsuC2I CCSGG 1 cut(s) 215
AvaII GGWCC 2 cut(s) 76, 115
BamHI GGATCC 1 cut(s) 268
BanII GRGCYC 1 cut(s) 171
BbrPI CACGTG 1 cut(s) 293
Bbv12I GWGCWC 1 cut(s) 171
BceAI ACGGC 2 cut(s) 169, 339
BciT130I CCWGG 1 cut(s) 243
BcnI CCSGG 1 cut(s) 215
BglI GCCNNNNNGGC 1 cut(s) 218
BlpI GCTNAGC 1 cut(s) 165
Bme1390I CCNGG 2 cut(s) 215, 243
Bme18I GGWCC 2 cut(s) 76, 115
BmgT120I GGNCC 3 cut(s) 76, 115, 235
BmiI GGNNCC 4 cut(s) 78, 117, 237, 270
BmrFI CCNGG 2 cut(s) 215, 243
BmsI GCATC 3 cut(s) 142, 154, 211
BpmI CTGGAG 1 cut(s) 284
Bpu1102I GCTNAGC 1 cut(s) 165
BpuMI CCSGG 1 cut(s) 215
BsaAI YACGTR 2 cut(s) 250, 293
BsaJI CCNNGG 2 cut(s) 213, 242
BsaWI WCCGGW 1 cut(s) 271
Bsc4I CCNNNNNNNGG 1 cut(s) 243
BseAI TCCGGA 1 cut(s) 271
BseBI CCWGG 1 cut(s) 243
BseDI CCNNGG 2 cut(s) 213, 242
BseGI GGATG 1 cut(s) 202
BseLI CCNNNNNNNGG 1 cut(s) 243
BseMII CTCAG 1 cut(s) 156
BshFI GGCC 2 cut(s) 175, 236
BsiHKAI GWGCWC 1 cut(s) 171
BsiSI CCGG 2 cut(s) 215, 272
BsiWI CGTACG 1 cut(s) 186
BslI CCNNNNNNNGG 1 cut(s) 243
BsnI GGCC 2 cut(s) 175, 236
Bsp1286I GDGCHC 1 cut(s) 171
Bsp13I TCCGGA 1 cut(s) 271
Bsp143I GATC 1 cut(s) 268
Bsp1720I GCTNAGC 1 cut(s) 165
BspACI CCGC 2 cut(s) 200, 253
BspANI GGCC 2 cut(s) 175, 236
BspCNI CTCAG 1 cut(s) 157
BspEI TCCGGA 1 cut(s) 271
BspLI GGNNCC 4 cut(s) 78, 117, 237, 270
BspPI GGATC 2 cut(s) 263, 276
BssECI CCNNGG 2 cut(s) 213, 242
BssMI GATC 1 cut(s) 268
BssNAI GTATAC 1 cut(s) 318
Bst1107I GTATAC 1 cut(s) 318
Bst2UI CCWGG 1 cut(s) 243
Bst4CI ACNGT 2 cut(s) 8, 100
Bst6I CTCTTC 1 cut(s) 19
BstBAI YACGTR 2 cut(s) 250, 293
BstC8I GCNNGC 2 cut(s) 92, 298
BstDEI CTNAG 1 cut(s) 165
BstF5I GGATG 1 cut(s) 202
BstKTI GATC 1 cut(s) 271
BstMBI GATC 1 cut(s) 268
BstMWI GCNNNNNNNGC 4 cut(s) 91, 181, 218, 259
BstNI CCWGG 1 cut(s) 243
BstSCI CCNGG 2 cut(s) 213, 241
BstX2I RGATCY 1 cut(s) 268
BstYI RGATCY 1 cut(s) 268
BstZ17I GTATAC 1 cut(s) 318
BsuRI GGCC 2 cut(s) 175, 236
BtsCI GGATG 1 cut(s) 202
BtsIMutI CAGTG 2 cut(s) 96, 303
Cac8I GCNNGC 2 cut(s) 92, 298
Cfr13I GGNCC 3 cut(s) 76, 115, 235
Csp6I GTAC 2 cut(s) 187, 247
CviAII CATG 2 cut(s) 49, 361
CviJI RGCY 9 cut(s) 94, 108, 164, 169, 175, 236, 278, 284, 300
CviKI_1 RGCY 9 cut(s) 94, 108, 164, 169, 175, 236, 278, 284, 300
CviQI GTAC 2 cut(s) 187, 247
DdeI CTNAG 1 cut(s) 165
DpnI GATC 1 cut(s) 270
DpnII GATC 1 cut(s) 268
Eam1104I CTCTTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 19
Ecl136II GAGCTC 1 cut(s) 169
Eco24I GRGCYC 1 cut(s) 171
Eco47I GGWCC 2 cut(s) 76, 115
Eco53kI GAGCTC 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 327
Eco72I CACGTG 1 cut(s) 293
EcoICRI GAGCTC 1 cut(s) 169
EcoO109I RGGNCCY 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 241
EcoT38I GRGCYC 1 cut(s) 171
FaeI CATG 2 cut(s) 52, 364
FaiI YATR 7 cut(s) 50, 111, 180, 223, 225, 318, 362
FatI CATG 2 cut(s) 48, 360
FauNDI CATATG 1 cut(s) 223
FblI GTMKAC 1 cut(s) 317
FokI GGATG 1 cut(s) 189
FriOI GRGCYC 1 cut(s) 171
GsuI CTGGAG 1 cut(s) 284
HaeIII GGCC 2 cut(s) 175, 236
HapII CCGG 2 cut(s) 215, 272
Hin1II CATG 2 cut(s) 52, 364
HinfI GANTC 2 cut(s) 57, 340
HpaII CCGG 2 cut(s) 215, 272
Hpy166II GTNNAC 3 cut(s) 76, 115, 318
Hpy188III TCNNGA 1 cut(s) 272
Hpy8I GTNNAC 3 cut(s) 76, 115, 318
Hpy99I CGWCG 1 cut(s) 325
HpyAV CCTTC 3 cut(s) 5, 154, 226
HpyCH4III ACNGT 2 cut(s) 8, 100
HpyCH4IV ACGT 3 cut(s) 249, 258, 292
HpyCH4V TGCA 3 cut(s) 43, 133, 296
HpyF10VI GCNNNNNNNGC 4 cut(s) 91, 181, 218, 259
HpyF3I CTNAG 1 cut(s) 165
HpySE526I ACGT 3 cut(s) 249, 258, 292
Hsp92II CATG 2 cut(s) 52, 364
Kpn2I TCCGGA 1 cut(s) 271
Kzo9I GATC 1 cut(s) 268
LmnI GCTCC 1 cut(s) 305
LpnPI CCDG 8 cut(s) 127, 157, 214, 228, 228, 248, 255, 285
LweI GCATC 3 cut(s) 142, 154, 211
MaeII ACGT 3 cut(s) 249, 258, 292
MaeIII GTNAC 2 cut(s) 58, 332
MalI GATC 1 cut(s) 270
MboI GATC 1 cut(s) 268
MboII GAAGA 3 cut(s) 36, 43, 169
MflI RGATCY 1 cut(s) 268
MhlI GDGCHC 1 cut(s) 171
MlyI GAGTC 1 cut(s) 66
MnlI CCTC 5 cut(s) 58, 64, 211, 259, 268
MroI TCCGGA 1 cut(s) 271
MslI CAYNNNNRTG 1 cut(s) 308
MspI CCGG 2 cut(s) 215, 272
MspR9I CCNGG 2 cut(s) 215, 243
MvaI CCWGG 1 cut(s) 243
MwoI GCNNNNNNNGC 4 cut(s) 91, 181, 218, 259
NciI CCSGG 1 cut(s) 215
NdeI CATATG 1 cut(s) 223
NdeII GATC 1 cut(s) 268
NlaIII CATG 2 cut(s) 52, 364
NlaIV GGNNCC 4 cut(s) 78, 117, 237, 270
NmuCI GTSAC 2 cut(s) 58, 332
OliI CACNNNNGTG 1 cut(s) 308
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PfeI GAWTC 1 cut(s) 340
Pfl23II CGTACG 1 cut(s) 186
PleI GAGTC 1 cut(s) 65
PmaCI CACGTG 1 cut(s) 293
PmlI CACGTG 1 cut(s) 293
PpsI GAGTC 1 cut(s) 65
Ppu21I YACGTR 2 cut(s) 250, 293
Psp124BI GAGCTC 1 cut(s) 171
Psp6I CCWGG 1 cut(s) 241
PspCI CACGTG 1 cut(s) 293
PspGI CCWGG 1 cut(s) 241
PspLI CGTACG 1 cut(s) 186
PspN4I GGNNCC 4 cut(s) 78, 117, 237, 270
PspPI GGNCC 3 cut(s) 76, 115, 235
PsuI RGATCY 1 cut(s) 268
RsaI GTAC 2 cut(s) 188, 248
RsaNI GTAC 2 cut(s) 187, 247
RseI CAYNNNNRTG 1 cut(s) 308
SacI GAGCTC 1 cut(s) 171
Sau3AI GATC 1 cut(s) 268
Sau96I GGNCC 3 cut(s) 76, 115, 235
SchI GAGTC 1 cut(s) 66
ScrFI CCNGG 2 cut(s) 215, 243
SduI GDGCHC 1 cut(s) 171
SetI ASST 9 cut(s) 69, 75, 96, 171, 244, 252, 261, 295, 302
SfaNI GCATC 3 cut(s) 142, 154, 211
SinI GGWCC 2 cut(s) 76, 115
SmiMI CAYNNNNRTG 1 cut(s) 308
SsiI CCGC 2 cut(s) 200, 253
SstI GAGCTC 1 cut(s) 171
StyD4I CCNGG 2 cut(s) 213, 241
TaaI ACNGT 2 cut(s) 8, 100
TaiI ACGT 3 cut(s) 252, 261, 295
TaqI TCGA 1 cut(s) 148
TfiI GAWTC 1 cut(s) 340
TscAI CASTG 2 cut(s) 103, 310
TseFI GTSAC 2 cut(s) 58, 332
Tsp45I GTSAC 2 cut(s) 58, 332
TspDTI ATGAA 1 cut(s) 65
TspGWI ACGGA 2 cut(s) 28, 77
TspRI CASTG 2 cut(s) 103, 310
VpaK11BI GGWCC 2 cut(s) 76, 115
XmiI GTMKAC 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.